23-08-2012 дата публикации
Номер: US20120214155A1
Принадлежит:
This disclosure relates to compositions and methods of their use in detection and identification of a chordopoxvirus in a sample, such as diagnosis of an infection in a subject. The compositions and methods allow for detection and identification of all non-avian low-GC content chordopoxviruses, identification of most known high-GC content chordopoxvirus, and species-specific detection of Canarypox virus, Fowlpox virus, and Sealpox virus. 1. An isolated nucleic acid molecule:a) comprising a nucleic acid sequence at least 80% identical to SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 28, or SEQ ID NO: 29;b) comprising at least 15 consecutive nucleotides of SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 28, or SEQ ID NO: 29; orc) selected from the group consisting of SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 28, and SEQ ID NO: 29.2. The isolated nucleic acid molecule of claim 1 , wherein the nucleic acid is an oligonucleotide.3. The isolated nucleic acid molecule of claim 1 , further comprising a detectable label.4. A method of identifying a chordopoxvirus in a sample that comprises nucleic acids claim 1 , the method comprising:generating an amplicon from the sample using an oligonucleotide primer pair comprising SEQ ID NOs: 13 and 14, or degenerate variants thereof; SEQ ID NOs: 20 and 21, or degenerate variants thereof; SEQ ID NOs: 22 and 23, or degenerate variants thereof; SEQ ID NOs: 25 and 26, or degenerate variants thereof; or SEQ ID NOs: 28 and 29, or degenerate variants thereof; anddetermining the identity of the amplicon;thereby identifying the chordopoxvirus.5. The method of claim 4 , wherein at least one of the oligonucleotide primers in the primer pair comprises a detectable label.6. The method of claim 4 ...
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