07-01-2015 дата публикации
Номер: CN104262469A
Принадлежит:
The invention discloses a method for separating and purifying pertactin. The method comprises the following steps: S1, sample treatment, namely, collecting a bordetella pertussis thallus, re-suspending, extracting and centrifuging the thallus to remove precipitate, carrying ultrafiltration and concentration on the supernate, and regulating the pH value being 7.0; S2, primary elution, namely, loading the supernate on a CaptoAdhere chromatographic column, eluting by a buffer solution, and collecting the eluent; S3, dialysis, namely, dialyzing and desalting the eluent in 15-25mM of a NaAc solution of which the pH is 4.5-5.5 to obtain dialysate; S4, secondary elution, namely, loading the dialysate on a CaptoSPImRes chromatographic column, eluting and collecting the eluent which is the purified pertactin. According to the method, the thallus of a bordetella pertussis nutrient solution is separated and purified by adopting the CaptoAdhere chromatographic column and the CaptoSPImRes chromatographic ...
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