16-07-2014 дата публикации
Номер: CN103923205A
Принадлежит:
The invention provides a method for separating and purifying uncoupling protein-1 in brown adipose tissues. The method comprises the following steps: mixing and grinding the brown adipose tissues of an experimental animal by an extracting solution A, centrifuging to remove adipose on the upper layer and taking primary supernate; centrifuging the primary supernate to remove precipitate, and taking secondary supernate; centrifuging the secondary supernate, taking primary precipitate, uniformly mixing the primary precipitate with an extracting solution B, carrying out ultrasonic treatment at 20kHz, centrifuging the solution subjected to ultrasonic treatment and taking secondary precipitate; uniformly mixing the secondary precipitate with the extracting solution B, adding TritonX-100, uniformly mixing, carrying out ultrasonic treatment at 20kHz, centrifuging the solution subjected to ultrasonic treatment, and taking third supernate; and filling supernate obtained three times to a column, eluting ...
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