11-02-2016 дата публикации
Номер: US20160038586A1
The invention is directed to dimeric fusion proteins and virus-like particles comprising such dimeric fusion proteins. These dimeric fusion proteins comprise an antigen or antigenic fragment carried between two viral structural proteins or fragments thereof, with or without linkers, in a manner that, relative to traditional monomeric platforms, minimizes steric hindrance among the antigen or antigenic fragment and the viral structural proteins or fragments thereof. This novel design provides for multivalent vaccines and enhanced immunogenicity. The invention also relates to nucleic acids encoding such dimeric fusion proteins and host cells comprising such nucleic acids. The invention further relates to pharmaceutical compositions comprising the dimeric fusion proteins and/or virus-like particles of the invention, and methods of prevention or treatment using such compositions. 1. A fusion protein comprising: V1 is an N-terminal viral structural protein,', 'L1 is an N-terminal linker,', 'Ag is an antigen or antigenic fragment of a pathogen,', 'L2 is a C-terminal linker, and', 'V2 is a C-terminal viral structural protein, and, '(a) V1-L1-Ag-L2-V2, wherein'}wherein each of V1 and V2 is, independently or together, capable of forming a virus-like particle; V1 is an N-terminal viral structural protein,', 'L1 is an N-terminal linker,', 'Ag is an antigen or antigenic fragment of a pathogen,', 'L2 is a C-terminal linker, and', 'V2 is a C-terminal viral structural protein, and, '(b) V1-L1-Ag-V2 or V1-Ag-L2-V2, wherein'}wherein each of V1 and V2 is, independently or together, capable of forming a virus-like particle; V1 is an N-terminal viral structural protein,', 'Ag is an antigen or antigenic fragment of a pathogen,', 'V2 is a C-terminal viral structural protein, and, '(c) V1-Ag-V2, wherein'}wherein each of V1 and V2 is, independently or together, capable of forming a virus-like particle; V1 is a fragment of an N-terminal viral structural protein,', 'L1 is an N-terminal ...
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