29-07-2021 дата публикации
Номер: US20210230563A1
Автор:
Chen Bo,
DING Dehui,
Li Huarong,
Li Zhenguo,
LIAN Lei,
LIU Guizhi,
MO Sudong,
Song Chao,
Wang Lei,
YUAN Guangdi,
Zhang Junjie
Принадлежит:
The present invention relates to a mutant p-hydroxyphenylpyruvate dioxygenase (HPPD) protein or a bioactive fragment thereof and an isolated polynucleotide comprising a nucleic acid sequence encoding the protein or fragment thereof, wherein the mutant p-hydroxyphenylpyruvate dioxygenase (HPPD) protein or a bioactive fragment thereof retains or enhances the property of catalyzing the conversion of p-hydroxyphenylpyruvate (HPP) to homogentisate and is significantly less sensitive to HPPD-inhibiting herbicides than a wild-type HPPD. The present invention also relates to a nucleic acid construct, an expression vector and a host cell comprising the polynucleotide, as well as to a method for producing a plant that has the property of catalyzing the conversion of p-hydroxyphenylpyruvate (HPP) to homogentisate and significantly reduced sensitivity to HPPD-inhibiting herbicides. 1. A mutant p-hydroxyphenylpyruvate dioxygenase (HPPD) protein or a bioactive fragment thereof , wherein , as compared to the amino acid sequence of a wild-type rice p-hydroxyphenylpyruvate dioxygenase protein , the amino acid sequence of the mutant p-hydroxyphenylpyruvate dioxygenase (HPPD) protein has one or more of mutations selected from the group consisting of 93S , 103S , 141R , 141K , 141T , 165V , 191I , 220K , 226H , 276W , 277N , 336D , 337A , 338D , 338S , 338Y , 342D , 346C , 346D , 346H , 346S , 346Y , 370N , 377C , 386T , 390I , 392L , 403G , 410I , 418P , 419F , 419L , 419V , 420S , 420T , 430G , and 431L at one or more positions corresponding to 93 , 103 , 141 , 165 , 191 , 220 , 226 , 276 , 277 , 336 , 337 , 338 , 342 , 346 , 370 , 377 , 386 , 390 , 392 , 403 , 410 , 418 , 419 , 420 , 430 and 431 of the amino acid sequence of wild-type rice p-hydroxyphenylpyruvate dioxygenase protein as set forth in SEQ ID NO: 2.2. The mutant p-hydroxyphenylpyruvate dioxygenase (HPPD) protein or a bioactive fragment thereof according to claim 1 , wherein the amino acid sequence of the mutant p- ...
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