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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 28. Отображено 28.
04-04-2023 дата публикации

Connecting frame, clothes processing device and clothes processing equipment

Номер: CN115897169A
Автор: SUN MINGCHAO, WU YUE, CHEN TAO, XIE HONGGANG, LUO ZHAOFENG
Принадлежит:

The invention discloses a connecting frame, a clothes processing device and clothes processing equipment, and the connecting frame is suitable for being arranged at a top opening of a first clothes processing unit so as to connect the first clothes processing unit and a second clothes processing unit together. According to the connecting frame provided by the embodiment of the invention, a top cover of a clothes washing unit or a clothes drying unit in the related technology is replaced, and a support does not need to be additionally arranged for connection, so that the number of parts is reduced, the overall height of a product is reduced, the number of assembly gaps is reduced, and the overall appearance quality of the product is improved. In addition, during assembly, screw mounting holes do not need to be punched in the clothes processing unit located below, the appearance of the product is guaranteed, the number of machining procedures is reduced, and therefore the assembly efficiency ...

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Номер записи: 1
23-06-2023 дата публикации

Aptamer sensor for detecting SARS-CoV-2S1 based on CRISPR/Cas12a

Номер: CN116297755A
Автор: ZHANG LIYUN, XING WENPING, LI QIAN, HAN CONG, ZHANG ZHENG, FANG XIAONA, LUO ZHAOFENG
Принадлежит:

The invention provides an electrochemical aptamer sensor for detecting SARS-CoV-2 S1 protein based on CRISPR (clustered regularly interspaced short palindromic repeats)/Cas12a (clustered regularly interspaced short palindromic repeats). The electrochemical aptamer sensor comprises an SARS-CoV-2 S protein aptamer, CRISPR/Cas12a protein, crRNA (complementary Ribonucleic Acid) and a gold electrode, wherein the surface of the gold electrode is modified by polyadenylic acid-methylene blue. According to the sensor disclosed by the invention, the nucleic acid aptamer is directly used as an activation chain of CRISPR/Cas12a, so that the addition of complementary chains is reduced; the biosensor integrates the advantages of electrochemistry and CRISPR/Cas12a, the shear capacity of CRISPR/Cas12a is amplified in the form of an electric signal, and the sensitivity of a detection system is improved. In addition, the aptamer provided by the invention has the potential of being combined with an S1 protein ...

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Номер записи: 2
27-08-2008 дата публикации

Non-mark phage exhibit protein chip as well as preparation and testing method thereof

Номер: CN0101251541A
Автор: LIU FANGFANG, LUO ZHAOFENG, DING XIANG, YU XINGLONG, FANGFANG LIU, ZHAOFENG LUO, XIANG DING, XINGLONG YU
Принадлежит:

The invention provides an unmarked bacteriophage display protein chip and a preparation and detection method thereof, wherein the unmarked bacteriophage display protein chip comprises a glass substrate, a chromium film, a gold film, a coupling layer and bacteriophage; the coupling layer is a layer of HS-(CH2)10-COOH self-assembly molecular film; the polypeptide segment or protein is displayed on the coat protein pIII or pVIII of the filobactivirus. The preparation method is based on the bacteriophage display pIII system or pVIII system, and the chip takes an optical glass flake coated with the gold film as the substrate, is fixed on the surface of a sensing chip by adopting the carboxyl covalent union and is combined with the surface plasma by the resonance and sensing; the invention also provides a detection method for the unmarked bacteriophage display protein chip. The invention can not only conveniently acquire various special identifying molecules to be used as probes, but also realize ...

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Номер записи: 3
10-12-2014 дата публикации

LED-based Multi-window and multi-path detection method of digital polymerase chain reaction PCR

Номер: CN104198392A
Автор: HE LIQUN, LUO ZHAOFENG, ZHANG HAIFENG
Принадлежит:

The invention provides an LED-based multi-window and multi-path detection method of digital polymerase chain reaction PCR. The method comprises the following steps: the LEDs are adopted as detection light sources, transparent windows are arranged at the tail end of a capillary pipeline in a segmentation manner after the digital PCR finishes polymerase chain reaction; each LED with one type of wavelength is responsible for detecting one window, a type of signal possibly sent by sample drops is detected. Since the drops of the digital PCR are ordered, multiple signals in each sample drop can be detected and accurately counted. Compared with the method of detecting the same drop by adopting multiple bundles of laser, the method is simpler and more feasible, has the characteristics of simple structure and low cost, and also can be used as an inserting piece to be combined with the existing detection equipment so as to increase the working speed of daily blood detection in a hospital.

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Номер записи: 4
24-11-2010 дата публикации

Phase measurement method of variable exposure time imaging phase shift

Номер: CN0101893563A
Автор: WEI ZHANG, XINGLONG YU, YAN DENG, ZHAOFENG LUO, ZHANG WEI, YU XINGLONG, DENG YAN, LUO ZHAOFENG
Принадлежит:

The invention relates to a phase measurement method of a variable exposure time imaging phase shift, belonging to the technical field of optical phase measurement. the method is characterized in that image-collecting software based on a variable exposure time imaging phase shift is established in a computer in an SPR (Surface Plasmon Resonance) imaging detection system which is modulated by a time phase, exposure time parameters including various different parameters, such as exposure time, exposure start time and exposure end time are set, and a phase shift measurement period, a frame synchronization period, an electro-optical crystal synchronization period and an exposure signal period including the exposure start time and the exposure end time are controlled by an FPGA (Field programmable Gate Array) preset in a CCD (Charge Coupled Device) camera to drive a CCD driving circuit so that images are shot one by one by a CCD chip, and the standard deviation of the phase error of p lightoutput ...

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Номер записи: 5
22-07-2009 дата публикации

Preparation of nitrilotriacetic acid affinity molecular layer

Номер: CN0101486662A
Автор: LUO ZHAOFENG, OU HUICHAO, ZHOU HONGMIN, JIANG HAO, JIANG HAIFENG, ZHAOFENG LUO, HUICHAO OU, HONGMIN ZHOU, HAO JIANG, HAIFENG JIANG
Принадлежит:

The invention discloses a preparation method of a nitrilotriacetic acid compatible molecular film, which is characterized in that the molecular film with carboxyl groups is placed in a mixed aqueous solution containing 0.01M to10M of N-hydroxy succinimide and 0.01M to10M of ethyl-dimethyl aminopropyl-carbodiimide for soaking for not less than one minute; the activated molecular film is placed in an aqueous solution containing 0.01mM to10M of nitrilotriacetic acid for soaking for not less than one minute; the above steps are repeated for more than one time; and the molecular film is placed in an aqueous solution containing 0.01M to10M of diethanolamine hydrochloride for soaking for not less than one minute. The preparation method can effectively strengthen the binding capability of compatible molecular films, has low preparation cost and simple and convenient operation, is applicable to various carried media, overcomes the defect that the binding capability of the nitrilotriacetic acid compatible ...

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Номер записи: 6
30-05-2023 дата публикации

Nucleic acid aptamer combined with IGFBP-2 protein and application thereof

Номер: CN116179556A
Автор: TAN WEIHONG, LI CHANYUAN, LUO ZHAOFENG, ZHANG ZHENG, LIAO GUOMIAO, LI LONGJIE, LEE JUN-CHAE
Принадлежит:

The invention discloses a nucleic acid aptamer combined with IGFBP-2 protein and application of the nucleic acid aptamer, and belongs to the technical field of biology. The nucleic acid aptamer is obtained through living body screening, and the sequence of the nucleic acid aptamer is as shown in SEQ ID NO. 1. The nucleic acid aptamer disclosed by the invention has the characteristics of high specificity, stable chemical property, easiness in preparation and storage, small molecular weight, high affinity, strong stability and the like; moreover, compared with in-vitro screening, the nucleic acid aptamer obtained by in-vivo screening has better stability and is more suitable for a specific target in a binding body.

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Номер записи: 7
23-06-2023 дата публикации

Nucleic acid aptamer SELEX (systematic evolution of ligands by exponential enrichment) technology combining cell screening and living body screening

Номер: CN116286832A
Автор: TAN WEIHONG, RAN CHUNYAN, LI LONGJIE, LI CHANYUAN, LIAO GUOMIAO, LUO ZHAOFENG
Принадлежит:

The invention discloses an aptamer SELEX (systematic evolution of ligands by exponential enrichment) technology combining cell screening and living body screening, which belongs to the technical field of biology and comprises the following steps: (1) constructing an unmodified screening library; (2) performing cell screening on the screening enrichment to obtain an enriched library; and (3) carrying out living body screening on the enriched library to obtain the aptamer. According to the aptamer SELEX technology, a cell screening and living body screening combined method is adopted, an unmodified original DNA library is adopted for screening, primary tumor cells of a tumor patient are firstly utilized for cell screening before living body screening, and living body screening is performed after an enriched library is preliminarily obtained, so that the success rate is greatly increased, and the screening efficiency is improved. And the nucleic acid aptamer with high stability and strong ...

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Номер записи: 8
16-05-2023 дата публикации

Nucleic acid aptamer combined with HGFR protein and application of nucleic acid aptamer

Номер: CN116121255A
Автор: TAN WEIHONG, LUO ZHAOFENG, ZHANG ZHENG, LIU XIANGSHENG, LYU SHAOKANG, DIAO HAN
Принадлежит:

According to the nucleic acid aptamer combined with the HGFR protein and the application of the nucleic acid aptamer, by improving screening conditions, the nucleic acid aptamer which is small in molecular weight, stable in chemical property, easy to store and mark and capable of being combined with the HGFR protein with high affinity is obtained through screening, and the nucleic acid aptamer can be combined with a cell line (verified by two cell lines) highly expressing the HGFR protein. After being truncated to 21-28 nucleotide lengths, the HGFR protein can still keep high affinity and high specificity binding with the HGFR protein, can be used for detection, diagnosis, imaging, treatment and the like, and has a wide application prospect.

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Номер записи: 9
06-04-2005 дата публикации

Surface plasma resonance rapid detection method for paralytic shellfish poisoning

Номер: CN0001603828A
Автор: WANG JUYING, LUO ZHAOFENG, BAO JUNBO, JUYING WANG, ZHAOFENG LUO, JUNBO BAO
Принадлежит:

This invention discloses a paralysis shellfish poison surface plasm resonance rapid measurement, which belongs to environment chemistry and biology molecule detecting field. The method is the following: to add quantitative PSP anti-body to the PSP sample solvent; then to flow on the gold sputtered plate with PSP solidified; to measure the over PSP anti-body in the solvent by use of plasm resonance sensor SPR detector to indirectly measure the concentration of the PSP solvent. This method is especially applied in quantitative study and regular or emergency monitor of PSP in freshwater algae or sea red tide. íí ...

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Номер записи: 10
09-06-2023 дата публикации

Sugar coating capable of prolonging drug release and preparation method thereof

Номер: CN116236453A
Автор: NI SHENG, LIU JIACONG, ZHANG QING, ZHANG LIANDI, CHEN PENG, LUO ZHAOFENG
Принадлежит:

The invention discloses a sugar coating capable of prolonging drug release and a preparation method of the sugar coating. The sugar coating comprises the following components: 1%-20% of API, 1%-10% of an adhesive, 40%-99% of cane sugar and 10%-50% of purified water. API is various water and ethanol insoluble raw materials. The adhesive is one or a composition of microcrystalline cellulose, hydroxypropyl methylcellulose, povidone, starch, gelatin and Arabic gum. According to the invention, microcrystalline cellulose with a special particle size is added into cane sugar, so that disintegration is prolonged, and optimal medication safety is achieved; by adding a trace amount of microcrystalline cellulose, the disintegration state is changed, absorption is delayed, and the effect of providing bioavailability is achieved. The sugar-coated tablet prepared by the method is relatively slow in drug release, and is suitable for most of drugs with narrow therapeutic windows and indissolvable; according ...

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Номер записи: 11
04-05-2011 дата публикации

Interference imaging method and system thereof for real-time detection of interaction of biological molecules

Номер: CN0102042972A
Автор: YAN DENG, DAQIAN WANG, WEI ZHANG, XINGLONG YU, ZHAOFENG LUO, DENG YAN, WANG DAQIAN, ZHANG WEI, YU XINGLONG, LUO ZHAOFENG
Принадлежит:

The invention relates to an interference imaging method and a system thereof for real-time detection of interaction of biological molecules, belonging to the field of biotechnologies; and the method comprises the following steps: light emitted by a light source is subject to collimation, polarization and beam expansion, and then is radiated into a sensing unit; the light reflected by the sensing unit passes through a half-wave plate, an imaging lens and a polarizing beam splitter prism, and then is divided into two paths of light, which are the transmitted light and the reflected light in theorthometric propagation directions, and further imaged on two CCDs (charge coupled devices); a computer is used for collecting two frames of interference images in real time, and an information diagram of refractive index distribution is obtained through resolution; and the sensing unit is measured continuously so as to obtain a real-time information diagram of the distribution of a series of refractive ...

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Номер записи: 12
18-01-2012 дата публикации

Biomolecule interaction detection method and system based on SPR interference imaging

Номер: CN0101915750B
Автор: DAQIAN WANG, YAN DENG, ZHAOFENG LUO, XINGLONG YU, WANG DAQIAN, DENG YAN, LUO ZHAOFENG, YU XINGLONG
Принадлежит:

The invention discloses a biomolecule interaction detection method comprising the following steps: 1) obtaining collimated light; 2) alternately modulating the phase difference between the P polarized component and the S polarized component of the collimated light to 90 degrees or -90 degrees; 3) allowing phase modulation light to enter into a biological sensing unit so as to stimulate surface plasma resonance, and reflecting light; 4) interfering the P polarized component with the S polarized component in the reflected light to generate an interference image; 5) imaging the interference image, and collecting the imaging interference image; 6) alternately collecting the imaging interference image generated after adding phase differences of 90 degrees and -90 degrees; and 7) utilizing the collected adjacent two frames of imaging interference images to calculate a distribution function reflecting the distribution of refractive index. The invention can continuously measure to obtain the refractive ...

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Номер записи: 13
20-03-2018 дата публикации

Lock structure of panel

Номер: CN0207122075U
Автор: LUO ZHAOFENG
Принадлежит: LUO ZHAOFENG

The utility model relates to a lock structure of panel, it includes fastener and the panel of swing joint in the fastener both sides, the one end of panel is equipped with coak, the both sides of fastener all be equipped with with panel coak assorted joggle spare, the end of joggle spare has buckling part no. 1, panel the position that corresponds buckling part no. 1 be equipped with with the buckling part no. 2 of a buckling part lock joint, when the panel swing joint of fastener both sides was on the fastener, the top surface of fastener both sides panel was located the coplanar, and betweenthe terminal surface of fastener both sides panel near. The utility model discloses a lock structure of panel, simple structure not only, the lock is simple and easy firm, does not need with the helpof the instrument when carrying out the lock moreover, does not carry out inseparable lock with the help of the instrument with panel even also can be on soft base (wall).

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Номер записи: 14
09-01-2008 дата публикации

Surface plasma resonance chip regeneration method

Номер: CN0101101289A
Автор: ZHAOFENG LUO, HUICHAO OU, LEIKE LI, YUCHUN CHEN, WEIWEI ZHANG, YUNFENG YAN, LUO ZHAOFENG, OU HUICHAO, LI LEIKE, CHEN YUCHUN, ZHANG WEIWEI, YAN YUNFENG
Принадлежит:

The character of the invention of the method of regeneration plasma resonant chip is that it deal with chip with mixed liquor of brimstone acid hyphen oxydol, after removing entirely the host of surface of chip, reforming the host of different types on the surface of chip by the technology of self-assembly of the faces of metal, finally making the chip to hook onto the chip set, and achieving rebirth of the old chip; the invention has the merit which is the types of rebirth chip unlimited and put in practice repeatedly, the chip after rebirthing can get the same performance as commercialization chip, resolving the problem of regeneration plasma resonant chip cannot recycling.

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Номер записи: 15
08-07-2009 дата публикации

Production method of surface plasma resonance chip

Номер: CN0101477114A
Автор: CUNXIAN SONG, HUICHAO OU, ZHAOFENG LUO, HONGMIN ZHOU, HAO JIANG, HAIFENG JIANG, SONG CUNXIAN, OU HUICHAO, LUO ZHAOFENG, ZHOU HONGMIN, JIANG HAO, JIANG HAIFENG
Принадлежит:

The invention discloses a preparation method of surface plasma resonance chips. The preparation method is characterized in that a disulfide bond reducing agent is adopted to modify biological protein molecules containing hydrosulfide group and/or disulfide bond; then, protein substrate is established on the surface of a chip by means of metal surface molecule self-assembly technology; and further modification is carried out to realize the preparation of a novel sensing chip used in SPR biosensor. The preparation method has the advantages of low preparation cost, simple operation, less time consumption and safety and harmlessness; moreover, the prepared chip has the functions of commercialized CM5 chips, thereby providing new possibilities for solving the prior problems of fewer types of surface plasma resonance chips and high cost.

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Номер записи: 16
11-03-2009 дата публикации

Use of Yunkang oral liquid in preparing medicine for treating kidney-deficiency type sterility

Номер: CN0101380440A
Автор: DACHONG WANG, ZHONGJIAN GUO, TIEFENG XUAN, ZHAOFENG LUO, XIAOSHENG YANG, WANG DACHONG, GUO ZHONGJIAN, XUAN TIEFENG, LUO ZHAOFENG, YANG XIAOSHENG
Принадлежит:

The invention relates to the application of Yunkang oral liquid in preparing kidney-deficiency type infertility medicine. The invention needs to solve the technical problem of the Yunkang oral liquid in preparing kidney-deficiency type infertility medicine to lead that the total effective rate is 60.46%. The traditional Chinese medicine compound formulation of the Yunkang oral liquid is prepared by the following steps: twenty two traditional Chinese medicines such as radix astragali, eucommia bark, radix codonopsitis, and the like, are soaked in warm water; four filtrate are combined; after colla corii asini is added, the mixture is concentrated to 1:1; ethanol is added to lead the amount of ethanol of the liquid medicine to be 70%; the mixture is rest; supernatant fluid is absorbed; the ethanol is recycled and filtration is carried out; filtrate is added with flavouring agent and sodium benzoate; water is added to adjust the pH value to be 5-6; water is added to adjust the total amount ...

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Номер записи: 17
17-02-2010 дата публикации

Health-care ginseng-aweto capsule

Номер: CN0101647569A
Автор: DACHONG WANG, ZHONGJIAN GUO, TIEFENG XUAN, ZHAOFENG LUO, WANG DACHONG, GUO ZHONGJIAN, XUAN TIEFENG, LUO ZHAOFENG
Принадлежит:

The invention relates to a health-care ginseng-aweto capsule for relieving physical fatigue. The technical problem of the invention is to provide a novel composition of ginseng and aweto powder, whichis a product having obvious effect on relieving physical fatigue. The ginseng-aweto capsule is characterized in that the ginseng-aweto capsule is prepared by pulverizing, screening and filling the raw materials in the following weight ratio: ginseng to aweto powder to panax ginseng leaves extract is equal to 50 to 40-50 to 4-6.

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Номер записи: 18
06-01-2010 дата публикации

Method for inhibiting protein intron activity and application thereof in medicine preparation

Номер: CN0101618046A
Автор: LIU YANGZHONG, ZHANG LIYUN, LUO ZHAOFENG, YANGZHONG LIU, LIYUN ZHANG, ZHAOFENG LUO
Принадлежит:

The invention provides a method for inhibiting protein intron activity, which is characterized by comprising the steps: acting a platinum composition on a microorganism with protein intron; inhibiting an internal protein cutting function of the microorganism by directly inhibiting the protein intron activity; thereby inhibiting the maturation and the function of the corresponding protein. The method for inhibiting protein intron activity has low use concentration of an inhibitor and wide application and is not easy to generate drug resistance. In masses of diseases which are related to microorganisms, the method for inhibiting protein intron activity has good application prospect on a tubercle bacillus resisting aspect particularly. The invention also provides the application of a platinum composition for preparing medicines which are used for treating diseases related to the protein intron activity, in particular to the application for preparing tuberculosis treating medicines.

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Номер записи: 19
04-03-2015 дата публикации

Microcapsule droplet generator and preparation method thereof

Номер: CN104383860A
Автор: JIAO DONGSHENG, HE LIQUN, LUO ZHAOFENG
Принадлежит:

The invention discloses a microcapsule droplet generator which is mainly formed by connecting three capillary glass tubes (1), (2) and (3) and two high-pressure T-shaped stainless steel tee joints (4) and (5), wherein the connection refers to that the three capillary glass tubes (1), (2) and (3) are coaxially fixed by virtue of the two high-pressure T-shaped stainless steel tee joints (4) and (5); and the capillary glass tubes are sealed by using silicone tubes (11) and (12). According to the micro-fluidic structure for producing microcapsules constructed based on the capillary glass tubes and the T-shaped high-pressure tee joints, a focused flow is constructed to produce microcapsules by virtue of the three capillary glass tubes of different diameters, the T-shaped joints have the fixing and sealing effects, and a micro-fluidic inlet channel is formed. According to the fixing effects of the T-shaped joints, the capillary tubes of small diameters are coaxially fixed in the capillary tubes ...

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Номер записи: 20
09-05-2023 дата публикации

Nucleic acid aptamer capable of specifically recognizing vancomycin and application of nucleic acid aptamer

Номер: CN116083433A
Автор: FANG XIAONA, HE LEI, QIU ZHIXIN, LUO ZHAOFENG
Принадлежит:

The invention discloses a nucleic acid aptamer for specifically recognizing vancomycin. The nucleotide sequence of the nucleic acid aptamer comprises at least one of the following three sequences: (1) a DNA sequence as shown in any one of SEQ ID NO: 1-3; (2) a DNA sequence which has more than 60% of homology with the DNA sequence as shown in any one of SEQ ID NO: 1-3 and is specifically combined with vancomycin; and (3) an RNA sequence which is transcribed by any DNA sequence as shown in SEQ ID NO: 1-3 and is specifically combined with vancomycin. The invention also discloses an application of the nucleic acid aptamer in detection of vancomycin. The nucleic acid aptamer disclosed by the invention has the characteristics of high specificity, stable chemical property and easiness in storage and marking, has the capability of being specifically combined with vancomycin, and can be applied to detection of vancomycin.

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Номер записи: 21
08-09-2010 дата публикации

Analysis method of solid phase micro-extraction surface plasma resonance on-line joint use

Номер: CN0101275885B
Автор: OU HUICHAO, JIANG HAIFENG, ZHOU HONGMIN, LUO ZHAOFENG, JIANG HAO, HUICHAO OU, HAIFENG JIANG, HONGMIN ZHOU, ZHAOFENG LUO, HAO JIANG
Принадлежит:

An analysis method for online combination of a solid phase microextraction and surface plasma resonance of the invention, characterized in that the method connects the covering capillary for the extraction selected by the target analyzing object to the between the sample inlet joint of the surface plasma resonance sensor and the micro-fluid ejecting plate; then the method orderly connects the quantitative water sample and the single clone antibody solution to the online combined analysis device via the joint which can achieve the online analysis of the organic matter of the water special track quantity; then the method orderly connects the single clone antibody solution of the target analyzing object and the rebirth liquor to the online combined analysis device, namely removes the residual track quantity organic mater and achieve the rebirth of the chip. The invention can be used for the analysis and detection of the pesticide residue, phenol, polychlorinated biphenyls, polycyclic aromatic ...

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Номер записи: 22
02-06-2023 дата публикации

Method for detecting residual solvent in acetylcysteine bulk drug

Номер: CN116203151A
Автор: NI SHENG, ZHAO DONG, ZHANG QING, ZHANG LIANDI, CHEN PENG, LUO ZHAOFENG
Принадлежит:

The invention discloses a method for detecting a residual solvent in an acetylcysteine bulk drug. The specific detection method comprises the following steps: step 1, preparing a reference substance solution of the residual solvent to be detected; 2, preparing a test solution; and 3, detecting the blank solution, the reference solution and the test solution by adopting liquid chromatography, recording a chromatogram, and calculating the residual quantity of acetic acid according to an external standard method. HPLC (High Performance Liquid Chromatography) and an ultraviolet detector are utilized, gradient elution is performed, and finally calculation is performed according to an external standard method. The high performance liquid chromatography is utilized to quickly and accurately detect acetic acid in acetylcysteine, the operation is simple, the practicability is strong, the sensitivity is good, the accuracy is high, and the residual quantity of acetic acid in the raw material medicine ...

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Номер записи: 23
07-01-2015 дата публикации

Flow polymerase chain reaction (PCR) circulating heating apparatus based on capillaries and heating method

Номер: CN104263634A
Автор: HE LIQUN, JIAO DONGSHENG, LUO ZHAOFENG
Принадлежит:

The invention discloses a flow polymerase chain reaction (PCR) circulating heating apparatus based on capillaries and a heating method. According to the method, PCR of samples in the capillaries is achieved by adopting a method that the capillaries penetrate through three different constant-temperature areas circularly, and a new process that amplification is achieved in a three-dimensional flow process of the samples under the condition of static temperature control can be formed. In a temperature cycling process, a fluid in each capillary carries sample droplets to flow into one end of a heating device, then penetrates among the three temperature areas along the capillary, and flows out from the heating device after completing a predetermined number of heating cycles to enter a detection device. Compared with a planar micro-chip method, the heating method disclosed by the invention is easily combined with existing biological detection equipment, and meanwhile, because the capillaries ...

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Номер записи: 24
10-02-2010 дата публикации

Reproducible molecule layer of biotin combined with protein and preparation method thereof

Номер: CN0101644711A
Автор: LUO ZHAOFENG, OU HUICHAO, ZHOU HONGMIN, JIANG HAO, JIANG HAIFENG, ZHAOFENG LUO, HUICHAO OU, HONGMIN ZHOU, HAO JIANG, HAIFENG JIANG
Принадлежит:

The invention discloses a reproducible molecule layer of biotin combined with protein and a preparation method thereof. The invention is characterized in that firstly a molecule layer with a triglycine structure on a carrier medium surface is put into an aqueous solution containing 0.00001-4M metal ions for soaking for not less than 10 seconds to obtain a molecule layer chelated with metal ions; then the molecule layer chelated with metal ions is put into an aqueous solution of biotin combined with protein which is of 0.00001-5mM and with not less than 6 groups of amino acid tags for soaking for not less than 10 seconds; the obtained reproducible molecule layer of biotin combined with protein has good stability and repetitiveness, can satisfy requirement of experiments with easy operationand mild reaction conditions, and is suitable for various carrier mediums; the invention overcomes the defect of un-reproducibility of the existing molecule layer of biotin combined with protein, andprovides ...

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Номер записи: 25
15-12-2010 дата публикации

Biomolecule interaction detection method and system based on SPR interference imaging

Номер: CN0101915750A
Автор: DAQIAN WANG, YAN DENG, ZHAOFENG LUO, XINGLONG YU, WANG DAQIAN, DENG YAN, LUO ZHAOFENG, YU XINGLONG
Принадлежит:

The invention discloses a biomolecule interaction detection method comprising the following steps: 1) obtaining collimated light; 2) alternately modulating the phase difference between the P polarized component and the S polarized component of the collimated light to 90 degrees or -90 degrees; 3) allowing phase modulation light to enter into a biological sensing unit so as to stimulate surface plasma resonance, and reflecting light; 4) interfering the P polarized component with the S polarized component in the reflected light to generate an interference image; 5) imaging the interference image, and collecting the imaging interference image; 6) alternately collecting the imaging interference image generated after adding phase differences of 90 degrees and -90 degrees; and 7) utilizing the collected adjacent two frames of imaging interference images to calculate a distribution function reflecting the distribution of refractive index. The invention can continuously measure to obtain the refractive ...

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Номер записи: 26
01-10-2008 дата публикации

Analysis method of solid phase micro-extraction surface plasma resonance on-line joint use

Номер: CN0101275885A
Автор: LUO ZHAOFENG, OU HUICHAO, ZHOU HONGMIN, JIANG HAO, JIANG HAIFENG, ZHAOFENG LUO, HUICHAO OU, HONGMIN ZHOU, HAO JIANG, HAIFENG JIANG
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An analysis method for online combination of a solid phase microextraction and surface plasma resonance of the invention, characterized in that the method connects the covering capillary for the extraction selected by the target analyzing object to the between the sample inlet joint of the surface plasma resonance sensor and the micro-fluid ejecting plate; then the method orderly connects the quantitative water sample and the single clone antibody solution to the online combined analysis device via the joint which can achieve the online analysis of the organic matter of the water special track quantity; then the method orderly connects the single clone antibody solution of the target analyzing object and the rebirth liquor to the online combined analysis device, namely removes the residual track quantity organic mater and achieve the rebirth of the chip. The invention can be used for the analysis and detection of the pesticide residue, phenol, polychlorinated biphenyls, polycyclic aromatic ...

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Номер записи: 27
19-11-2008 дата публикации

D-type and / or L-type phenyl-lactic acid rapid checking method

Номер: CN0101308124A
Автор: MU WANMENG, JIANG BO, LUO ZHAOFENG, ZHOU HONGMIN, WANMENG MU, BO JIANG, ZHAOFENG LUO, HONGMIN ZHOU
Принадлежит:

Disclosed is a fast detection method for D-type and/or L-type phenyl lactic acid, which belongs to the biological preservative detection method technique filed. The detection method separates D-type or L-type phenyl lactic acid from impurities of a sample to be detected by utilizing a chiral column by means of high efficiency liquid chromatography, and implements the qualitative and quantitative detection to D-type and/or L-type phenyl lactic acid respectively through the retention time and peak area of the high efficiency liquid chromatography by means of ultraviolet light absorption on-line detection. The detection method has advantages of fast detection, accurate quantification, good reproducibility; and broad detecting range of samples; and the method can be used for the qualitative and quantitative detection of phenyl lactic acid in fermentation production and research as well as dosage detection of phenyl lactic acid as biological preservative in the food industry.

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Номер записи: 28