04-02-2016 дата публикации
Номер: US20160030549A1
Принадлежит:
In certain embodiments, the disclosure relates to the polynucleotide sequences of respiratory syncytial virus (RSV). In certain embodiments, the disclosure relates to isolated or recombinant nucleic acids and polypeptides comprising desirable nucleic acid sequences and mutations disclosed herein. In certain embodiments, isolated or recombinant RSV comprising the nucleic acids and polypeptides disclosed herein (e.g., attenuated recombinant RSV) are also provided, as are immunogenic compositions including such nucleic acids, polypeptides, and RSV genomes that are suitable for use as vaccines. Attenuated or killed RSV containing these nucleic acids and mutation in the form of copied nucleic acids (e.g., cDNAs) are also contemplated. 1. An isolated recombinant nucleic acid encoding NS1 and/or NS2 of a wild-type human RSV or variant wherein the nucleotides are substituted such that a codon to produce Gly is GGT , a codon to produce Asp is GAT , a codon to produce Glu is GAA , a codon to produce His is CAT , a codon to produce Ile is ATA , a codon to produce Lys is AAA , a codon to produce Leu is CTA , a codon to produce Asn is AAT , a codon to produce Gln is CAA , a codon to produce Val is GTA , or a codon to produce Tyr is TAT , or combinations thereof.2. The isolated recombinant nucleic acid of further comprising a combination of at least two claim 1 , three claim 1 , four claim 1 , five claim 1 , six claim 1 , seven claim 1 , eight nine claim 1 , ten claim 1 , or all of the individual codons.3. The isolated recombinant nucleic acid of claim 2 , comprising at least 20 claim 2 , 30 claim 2 , 40 claim 2 , or 50 or more of the codons.4. The isolated recombinant nucleic acid of claim 1 , wherein the nucleotides are substituted such that a codon to produce Ala is GCG claim 1 , a codon to produce Cys is TGT claim 1 , a codon to produce Phe is TTT claim 1 , a codon to produce Pro is CCG claim 1 , a codon to produce Arg is CGT claim 1 , a codon to produce Ser is TCG claim 1 , ...
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