Preparation method and application of adenovirus-alphavirus hybrid virus

The invention relates to a preparation method and application of an adenovirus-alphavirus hybrid virus, belonging to the field of biotechnology and gene therapy. The preparation method comprises the steps of: constructing an alphavirus replicon vector carrying a target gene, constructing a hybrid shuttle plasmid vector, constructing a recombinant hybrid virus plasmid, rescuing a hybrid virus and checking functions. The hybrid virus prepared by using a system has particle features of Ad5/F11p and can effectively target and enter hematopoietic cells; after the virus enters the cells, an alphavirus replicon relevant element can effectively mediate efficient expression of the target gene, and a promoter CD11Ap can enhance the specificity of the hematopoietic cells. A hybrid virus preparation system can bring convenience for realizing the purpose of preparing the hybrid virus carrying different target genes through the replacement of the target gene in the alphavirus replicon vector. The hybrid ...

Method for preparing epothilones B and use thereof

The invention relates to a preparation method of epothilones B. In particularly, the invention provides a technique method to prepare epothilones B through adopting 100 to 500 liters fermentor to ferment and to separate epothilones B in large scale. The invention also relates to the preparation and application of epothilones B in preparation of gleevec resistance chronic myeloid leukemia treatment drug.

Method for preparing epothilones B

The invention relates to a preparation method of epothilones B. In particularly, the invention provides a technique method to prepare epothilones B through adopting 100 to 500 liters fermentor to ferment and to separate epothilones B in large scale. The invention also relates to the preparation and application of epothilones B in preparation of gleevec resistance chronic myeloid leukemia treatment drug.

Multifunctional agent and relevant processing technology for regeneration of wast accumulator plates

Application of epothilone B

The invention relates to application of epothilone B in preparing a medicament for treating Gleevec medicament resistant chronic myelogenous leukemia.

MULTIFUNCTIONAL AGENT AND RELEVANT PROCESSING TECHNOLOGY FOR REGENERATION OF WASTE ACCUMULATOR PLATES

The multifunctional agent contains by weight calcium hydroxide 0.5-5%, acidolytic barium hydroxide 2-4.5%, sodium hydroxide 3-4.5%, potassium hydroxide 3-7%, lithium hydroxid 3-5.5%, acidolytic magnesium oxide 1-3.5%, lithium carbonate 0.3-4%, and silicon deoxide 1.2-3.5%, and it is produced by compounding, mixing and stirring into pulp. The multifunctional agent may be used for plate regeneration in accumulator repairation and it has the functions of preventing plate from corrosion of suplhate and restraining the drop, solidification, volume increase and precipitation of positive-pole active substance.

Crank-connecting rod mechanism for engine of miniature cross-country motorcycle

The invention discloses a crank-connecting rod mechanism for an engine of a miniature cross-country motorcycle. The crank-connecting rod mechanism comprises a left crank, a right crank, a crank pin and a connecting rod, wherein the big head end of the connecting rod is positioned between the left crank and the right crank; the big head end of the connecting rod, the left crank and the right crank are connected with each other through the crank pin; the axial lines of the left crank and the right crank are in the same line and are parallel to the axial line of the crank pin; the distance between the axial lines of the left crank and the right crank and the axial line of the crank pin is 28.5 to 30.5 mm; the diameter of the shaft part of the right crank is 95 to 97 mm; the axial length of the right crank is 64 to 66 mm; and the distance between the centre of an installing hole at the big head end of the connecting rod and the centre of the installing hole at the small head end of the connecting ...

Application of epothilone B

The invention relates to application of epothilone B in preparing a medicament for treating Gleevec medicament resistant chronic myelogenous leukemia.

Needle bearing holder structure for engine

The invention discloses a motor used needle bearing cage structure which comprises two parallelly arranged outer rings (1) and beams (9) connecting the two outer rings (1); wherein the beams are uniformly distributed circumferentially; a through groove (3) is opened along the width direction on the top surface of the beam (2); two lateral walls of the through groove (3) are respectively close to inner end surfaces of the two outer rings (1). By slightly modifying the beam, the structure can effectively prevent the needle bearing cage from wearing and deforming and greatly improve the service performance of a crank-connecting rod and an entire motor. The structure is characterized by ingenious design, simple structure, easy implementation, etc.

Temperature sensing equipment of engine

The invention discloses a temperature sensor for an engine, which comprises an oil-groove-port end cover, and is characterized in that, the central opening at the cover top of the oil-groove-port end cover is connected with the outside of a temperature sensor; the outside of the temperature sensor is a cylindrical body with a countersunk hole, whose open end is opposite to the oil-groove-port end cover; in the countersunk hole, an insulation sealing block is shrink fit; in the insulation sealing block, a first junction strip and a second junction strip are inserted. Wherein, one end of the first junction strip passes through the insulation sealing block and stretches into the cavity at bottom of the countersunk hole; one end of the second junction strip is provided with a temp-sensing sheet metal, which stretches into the cavity at bottom of the countersunk hole, and is near to the first junction strip. The obvious techical benefits with the invention are: can provide control signals for ...

Motorcycle double-control digital ignition apparatus

The invention provides a double control digital igniter for a motorcycle, comprising a trigger signal processing circuit (3), a singlechip circuit (2), an ignition drive circuit (4) and a power supply circuit (5). The invention is characterized in that the singlechip circuit (2) is also provided with a load signal input end (GP1) which is connected with an output end of a load signal processing circuit (1), and an input end of the load signal processing circuit (1) is connected with a signal output end of a throttle opening sensor (6) of a carburetor of an engine of the motorcycle. The double control digital igniter can regulate an ignition advance angle according to rotating speed and loading of the engine, so that the engine can always maintain the best ignition time under various operating conditions, and the ignition advance angle can be inherently regulated to the best state according to changes of the loading and the rotating speed in the whole range of the rotating speed, thereby ...