22-05-2014 дата публикации
Номер: US20140143906A1
A method for improving plant insect resistance includes providing target genes derived from insects. Constructs based on the nucleotide sequences of these target genes are designed to form interfering molecules. After being eating by insects, the plant can significantly inhibit expression of corresponding genes in insects. This inhibition is not affected by barrier of the insect digestive systems. The method can improve the insect resistance of plants, reduce pesticide applications, lower the costs of agricultural production, and protect the environment. 1. A method for improving insect resistance in a plant , characterized in that the method comprises:expressing an interfering molecule that specifically interferes with the expression of an insect gene in the plant, wherein said insect gene is selected from trypsin precursor gene, dopa decarboxylase gene, NADH dehydrogenase ubiquinone flavoprotein 2 gene, ATP synthase beta subunit gene, or myosin heavy chain isoform B gene.2. (canceled)3. The method according to claim 1 , characterized in that said interfering molecule is selected from: a dsRNA claim 1 , an antisense nucleic acid claim 1 , a small interfering RNA claim 1 , or an miRNA targeting said insect gene or its fragment or a transcript thereof for inhibiting or silencing that target gene.5. The method according to claim 4 , characterized in that step (2) comprises:{'i': 'Agrobacterium tumefaciens', '(a) providing carrying an expression vector, wherein said expression vector contains said construct of (1);'}{'i': 'Agrobacterium tumefaciens', '(b) contacting a plant cell, a plant tissue, or a plant organ with of step (a), thereby transfecting said construct into the plant cell, the plant tissue, or the plant organ.'}6. The method according to claim 4 , characterized in that said Seqis selected from:SEQ ID NO: 1 or the sequence located at nucleotides 1-576 thereof;SEQ ID NO: 2 or the sequence located at nucleotides 311-621 thereof;SEQ ID NO: 3 or the sequence ...
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