26-04-2016 дата публикации
Номер: KR1020160044695A
Автор:
YUN, HYUN SHIK,
PARK, HYE MIN,
KIM, SE KYUNG,
DO, KYUNG HEE,
JEONG, SU YEON,
LEE, SUN HEE,
LEE, JIN WON
Принадлежит:
The present invention relates to recombinant E. coli producing, among unsaturated fatty acids, cis-vaccenic acid; and to a method for producing unsaturated fatty acids using the same. The recombinant E. coli producing unsaturated fatty acids according to the present invention constructs a system co-expressing 5 genes, i.e., accA, accB, accC, accD, fabD, and fabD located in one plasmid; 3 genes, i.e., fabB, fabF, and fabA, located in one plasmid; and tesA located in one plasmid. Also, the four recombinant plasmids are introduced into the recombinant E. coli (BL21(DE3) ΔfadD), which is a host strain having alleviated fatty acid reduction by removing fadD in a beta-oxidation step of fatty acid synthesis, and causes simultaneous transformation leading to a co-expression of 13 genes. In addition, when fatty acids are prepared by culturing the recombinant E. coli at a low temperature, the ratio and quantity of vaccenic acid, among unsaturated fatty acids, are greatly increased, so the unsaturated ...
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