Method for detecting vanillylmandelic acid and homovanillic acid in urine and application of method

The invention provides a method for detecting vanillylmandelic acid and homovanillic acid in urine and application of the method, and relates to the technical field of metabolite detection. The detection method comprises the following steps: detecting a pretreated to-be-detected sample by using high performance liquid chromatography, substituting a detection value into a standard curve, and calculating to obtain the content of vanillylmandelic acid and homovanillic acid in the urine sample, the pretreatment comprises the step of extracting a to-be-detected sample by using an extraction liquid containing an organic phase; and detecting the extracted sample to be detected by adopting high performance liquid chromatography. According to the detection method, the technical problems that in the prior art, the detection cost of vanillylmandelic acid and homovanillic acid in urine is high, and the detection method is complex are solved.