СПОСОБ И УСТРОЙСТВО ДЛЯ ОПРЕДЕЛЕНИЯ ПАРАМЕТРОВ СИСТЕМЫ В ЦЕЛЯХ УМЕНЬШЕНИЯ КОРРОЗИИ В УСТАНОВКЕ ПЕРВИЧНОЙ ОБРАБОТКИ НЕФТИ

Изобретение относится к определению количества различных веществ в жидком образце. В устройстве используется по крайней мере один способ оптического анализа, не зависящий от объема и/или концентрации, для определения одного из следующих свойств: водородного показателя pH, количества хлорида и/или количества железа в образце. Оптическое свойство может быть колориметрическим, флуоресцентным или и тем, и другим, причем оно может являться результатом добавления в образец красителей, комплексообразующих агентов, соединений, вызывающих мутность, и других реагентов, вызывающих оптический эффект. Способ также включает использование электрода BDD для окисления веществ (таких как сульфоксидные соединения), которые в противном случае мешали бы проведению оптического анализа, и/или для промывания образца газом. Так как измерения не зависят от концентрации и объема, их можно проводить непрерывно, быстро, тем самым избегая неудобства начала и завершения технологического процесса, а устройство может многократно ...

ПОРТАТИВНОЕ УСТРОЙСТВО ДЛЯ ПРОВЕРКИ АЛКОГОЛЬНОГО НАПИТКА ЧЕРЕЗ КОНТЕЙНЕР, СИСТЕМА И СВЯЗАННЫЙ С НИМИ СПОСОБ

Изобретение относится к области аутентификации содержимого, такого как напитка, в частности алкогольного напитка. Заявленное портативное устройство для проверки алкогольного напитка в по меньшей мере частично прозрачном контейнере содержит: одиночный источник света, выполненный с возможностью испускания луча монохромного возбуждающего света, имеющего длину волны в диапазоне от 350 до 650 нм; разделитель луча, сориентированный под 45° относительно направления испускания источника света для отражения луча возбуждающего света; фокусную и собирающую линзу; позиционирующее устройство, обеспечивающее ориентацию луча света, исходящего от источника света, по направлению, по существу нормальному к внешней поверхности контейнера. Причем позиционирующее устройство также обеспечивает позиционирование внешней поверхности контейнера на заранее определенном расстоянии от фокусной линзы, при этом заранее определенное расстояние между фокусной линзой и контейнером выбрано таким образом, что луч света сфокусирован ...

ПОРТАТИВНЫЙ ДЕТЕКТОР ИНТЕНСИВНОСТИ ФЛУОРЕСЦЕНЦИИ, ВОЗБУЖДАЕМОЙ УЛЬТРАФИОЛЕТОВЫМ ИЗЛУЧЕНИЕМ

Изобретение относится к области измерительной техники и касается портативного детектора интенсивности флуоресценции. Детектор предназначен для выполнения количественного определения флуоресценции исходных материалов одноразового гигиенического изделия и содержит корпус с измерительным отверстием, источник возбуждающего флуоресценцию ультрафиолетового излучения, два датчика флуоресцентного излучения и два фильтрующих устройства. Детектор выполнен таким образом, что путь света ультрафиолетового излучения и пути света первого и второго принимаемых излучений флуоресценции являются копланарными и при размещении детектора на плоской подложке сходятся в точке на ее поверхности. Первый и второй датчики могут быть избирательно приведены в действие и предназначены для преобразования света с длинами волн соответственно от 420 до 480 нм и от 480 до 760 нм. Технический результат заключается в ускорении процесса измерений и обеспечении возможности количественного определения интенсивности флуоресценции ...

УСТРОЙСТВО ДЛЯ МОНИТОРИНГА МНОЖЕСТВА ДИСКРЕТНЫХ СИГНАЛОВ ФЛУОРЕСЦЕНЦИИ

Изобретение предназначено для мониторинга множества дискретных сигналов флуоресценции, в частности для секвенирования ДНК посредством использования нуклеотидов с флуоресцентной меткой. Детектор (118) содержит множество пикселей (130) для отдельного детектирования упомянутых сигналов флуоресценции из множества источников (104) флуоресцентных сигналов, при этом каждый пиксель (130) содержит предварительно определенное число из, по меньшей мере, двух элементов (D1, Dn) детектирования для детектирования принимаемого флуоресцентного сигнала и для формирования сигналов детектирования, а также схему (140) преобразования сигналов для приема упомянутых сигналов детектирования из упомянутых, по меньшей мере, двух элементов (D1, Dn) детектирования и для формирования пиксельного выходного сигнала, указывающего, какой из упомянутых, по меньшей мере, двух элементов (D1, Dn) детектирования сформировал самый сильный сигнал детектирования. 2 н. и 13 з.п. ф-лы, 7 ил.

СПОСОБ И УСТРОЙСТВО ДЛЯ ФЛУОРЕСЦЕНТНОЙ СПЕКТРОМЕТРИИ В СКВАЖИНЕ

Прибор для измерения флуоресценции пластового флюида в стволе скважины содержит камеру с окошком, сообщающимся своей одной стороной с пластовым флюидом, другой стороной с источником ультрафиолетового излучения. Источник ультрафиолетового излучения для освещения пробы флюида и приемник излучения для измерения спектров флуоресценции пробы флюида расположены со второй стороны окошка. Также предложен способ для измерения флуоресценции пластового флюида в стволе скважины. Изобретение обеспечивает упрощение устройства и способа, а также повышение их надежности. 2 н. и 19 з.п. ф-лы, 3 ил.

ПОРТАТИВНЫЙ ДЕТЕКТОР ИНТЕНСИВНОСТИ ФЛУОРЕСЦЕНЦИИ, ВОЗБУЖДАЕМОЙ УЛЬТРАФИОЛЕТОВЫМ ИЗЛУЧЕНИЕМ

СИСТЕМА И СПОСОБ ОНЛАЙНОВОГО АНАЛИЗА И СОРТИРОВКИ СВОЙСТВ СВЕРТЫВАНИЯ МОЛОКА

... 1. Способ онлайнового направления молока на основании прогнозируемых будущих свойств коагуляции, причем способ включает: ! отбор сырого молока для получения проб сырого молока во время протекания сырого молока в молочной линии между постом дойки для доения животного и множеством пунктов сбора для сбора молока, полученного от этого животного; ! выполнение анализа пробы сырого молока; ! прогнозирование по меньшей мере одного будущего параметра коагуляции в онлайновом режиме во время протекания по молочной линии на основании анализа; и направление сырого молока во время протекания по молочной линии в одно из множества пунктов сбора на основании по меньшей мере одного будущего параметра коагуляции. ! 2. Способ по п.1, включающий смыв пробы сырого молока вместе с сырым молоком в одно из множества пунктов сбора на основании по меньшей мере одного прогнозируемого будущего параметра коагуляции. ! 3. Способ по п.1 или 2, отличающийся тем, что анализ основан на по меньшей мере одном из спектрального ...

БИОМАРКЕРЫ ИНФЕКЦИЙ ДЫХАТЕЛЬНЫХ ПУТЕЙ

... 1. Способ определения уровня активности бактерий в легких пациента, где указанный способ включает:осуществление первого измерения в первый момент времени уровня, как минимум, одного маркера процесса захвата железа бактериями и, как минимум, одного секретируемого бактериального белка в образце мокроты из легких;осуществление второго измерения во второй момент времени уровней указанного маркера и указанного токсина в образце мокроты из легких; иопределение упомянутого уровня бактериальной активности по изменению с течением времени измеренных уровней указанного маркера процесса захвата железа бактериями и указанного секретируемого бактериального токсина.2. Способ по п. 1, включающий измерение уровней нескольких указанных маркеров процесса захвата железа бактериями и определения указанного уровня бактериальной активности, исходя из указанных нескольких измеренных уровней.3. Способ по п. 1 или 2, где указанный маркер (маркеры) включает сидерофор (сидерофоры), в частности один или несколько сидерофоров ...

СПОСОБ И УСТРОЙСТВО ДЛЯ ОПРЕДЕЛЕНИЯ ПАРАМЕТРОВ СИСТЕМЫ В ЦЕЛЯХ УМЕНЬШЕНИЯ КОРРОЗИИ В УСТАНОВКЕ ПЕРВИЧНОЙ ОБРАБОТКИ НЕФТИ

... 1. Способ измерения по крайней мере одного свойства преимущественно жидкого образца, включающий следующие этапы:добавление по крайней мере одного химического реагента к образцу, причем указанный химический реагент, когда его добавляют к образцу, способен вызывать измеряемый оптический эффект, непосредственно связанный с определяемым свойством,измерение указанного оптического эффекта, ивыведение значения свойства путем сравнения измеренного оптического эффекта с заранее определенными значениями, соответствующими определяемому свойству,удаление сульфоксидных соединений из образца путем добавления к нему промывной смеси, причем указанная промывная смесь содержит 2-10% перуксусную кислоту в равновесии с 10-35% перекисью водорода и воду,причем отношение между измеренным оптическим эффектом и определяемым свойством не зависит от объема жидкого образца и от объема реагента, добавленного к образцу, причем промывная смесь не оказывает воздействие на измеряемый оптический эффект за исключением предотвращения ...

СКАНИРУЮЩИЙ В РЕЖИМЕ РЕАЛЬНОГО ВРЕМЕНИ МИКРОЖИДКОСТНЫЙ ТЕРМОЦИКЛЕР И СПОСОБЫ СИНХРОНИЗИРОВАННЫХ ТЕРМОЦИКЛИРОВАНИЯ И СКАНИРУЮЩЕГО ОПТИЧЕСКОГО ДЕТЕКТИРОВАНИЯ

... 1. Устройство для амплификации и детектирования нуклеиновой кислоты в режиме реального времени, содержащееголовку детектора, содержащую множество пар источников света с фотодетекторами, причемголовка детектора размещена на направляющей, апары фотодетекторов с источниками света выровнены в виде первого ряда и второго ряда;гнездо для микрожидкостного картриджа, содержащего множество независимых реакционных камер, ориентированных в соседних столбцах первого ряда и второго ряда, иапертурную пластину, выполненную с возможностью размещения над микрожидкостным картриджем при наличии картриджа в гнезде, при этом головка детектора размещена над апертурной пластиной,причем апертурная пластина содержит множество апертурных отверстий, каждое из которых размещено с выравниванием над каждой камерой из множества реакционных камер при удержании микрожидкостного картриджа в гнезде, аголовка детектора выполнена с возможностью перемещения вдоль указанной направляющей, так что каждая пара из множества пар ...

Mehrfarbige-Fluoreszenz-Analysevorrichtung

Eine Mehrfarbige-Fluoreszenz-Analysevorrichtung 1 gemäß der vorliegenden Erfindung ist mit Folgendem versehen: einer Bestrahlungseinheit 41 zum Einstrahlen von Anregungslicht mit mehreren Anregungswellenlängenbändern auf eine Probe s, einer ersten faseroptischen Platte 424 zum Leiten von Licht einschließlich der von der Probe s durch die Bestrahlung mit dem Anregungslicht emittierten Fluoreszenz und zum Emittieren von diesem von einem ersten Emissionsteil 425b, einer zweiten faseroptischen Platte 431 zum Empfangen vom ersten Emissionsteil 425b emittierten Lichts an einem zweiten Einfallsteil 434a, zum Leiten von diesem und zum Emittieren von diesem von einem zweiten Emissionsteil 434b, einem einzelnen Dielektrischer-Mehrschichtfilm-Interferenzfilter 432, das an einer Endfläche des zweiten Emissionsteils 434b bereitgestellt ist, zumindest einen Teil der Fluoreszenz durchlässt und Licht aus mehreren Transmissionswellenlängenbändern, welche die Anregungswellenlängenbänder nicht einschließen ...

VORRICHTUNG UND VERFAHREN ZUM HOCHAUFLÖSENDEN BOHRLOCHSPEKTROMETER

Ein System umfassend einen Biochip und einen Biochip-Lesegerät

INDIREKTE KOLORIMETRISCHE DETEKTION EINES ANALYTEN IN EINER PROBE UNTER ZUHILFENAHME DES VERHAELTNISSES VON LICHTSIGNALEN.

Mikroskopische Einrichtung und Verfahren zur dreidimensionalen Lokalisierung von punktförmigen Objekten in einer Probe

Beschrieben ist eine mikroskopische Einrichtung (100) zur dreidimensionalen Lokalisierung von punktförmigen Objekten, umfassend eine Detektionsoptik (36, 108), die in punktförmige Objekte (30, 114) jeweils in Form einer dreidimensionalen Fokuslichtverteilung in einen Bildraum abbildet, eine Farbtrennvorrichtung (126), die das Licht in mindestens zwei separate Lichtbündel teilt, deren Lichtwellenlängen in unterschiedlichen Wellenlängenbereichen liegen, mindestens zwei in dem Bildraum angeordnete Detektoreinheiten (146, 148), von denen eine Detektoreinheit das eine der beiden Lichtbündel und die andere Detektoreinheit das andere der beiden Lichtbündel empfängt, wobei jede Detektoreinheit (146, 148) eine Detektionsfläche (38, 150, 152) zum Erfassen von Lichtflecken (42) aufweist, eine Auswerteeinheit (154), die durch Auswerten des jeweiligen auf der jeweiligen Detektionsfläche (38, 150, 152) erfassten Lichtflecks (42) eine laterale x-y-Position des zugehörigen punktförmigen Objektes (30, 114 ...

Verfahren und Vorrichtung zur Detektion von Fremdstoffen in Wasser

Die Erfindung betrifft ein Verfahren zur Detektion von Fremdstoffen, insbesondere von Schadstoffen, in Wasser, insbesondere in Trinkwasser, vorzugsweise geeignet zur Durchführung mit einer Vorrichtung gemäß einem der Ansprüche 1015, umfassend die Schritte a) Entnehmen einer Probe, b) Aufbereiten der entnommenen Probe, wobei das Aufbereiten der Probe eine zumindest teilweise Destillation der entnommenen Probe umfasst, c) Analysieren der aufbereiteten Probe mittels Absorptions- und/oder Emissions-Spektroskopie, wobei das Analysieren die folgenden Schritte umfasst: c1) Anregen der aufbereiteten Probe mit Licht, insbesondere eines Lasers oder einer Deuteriumlampe, mit zumindest einer Anregungswellenlänge, und c2) Aufnehmen zumindest eines Absorptions- und/oder Emissionsspektrums des durch die Probe transmittierten und/oder des von der Probe emittieren Lichts mittels eines Detektors, und d) Ermitteln zumindest eines der Fremdstoffe in der analysierten Probe anhand zumindest eines der aufgenommenen ...

Verfahren und Vorrichtung zum räumlichen Messen nanoskaliger Strukturen

Verfahren zum räumlichen Messen mehrerer nanoskaliger Strukturen in einer Probe (13) mit den Schritten:- Markieren (1) der einzelnen Strukturen an verschiedenen Stellen mit Fluoreszenzmarkern (8, 9),- Anregen der Fluoreszenzmarker (8, 9) mit Anregungslicht (25) zur Emission von Fluoreszenzlicht (42), wobei eine Intensitätsverteilung (24) des Anregungslichts (25) oder eine Intensitätsverteilung (48) von weiterem Licht, das sich auf die Emission von Fluoreszenzlicht durch die Fluoreszenzmarker (8, 9) auswirkt, ein lokales Minimum (26) aufweist, das an verschiedenen Positionen in der Probe (13) angeordnet wird,- Registrieren (4) des aus der Probe (13) emittierten Fluoreszenzlichts (42) getrennt für die einzelnen Fluoreszenzmarker (8, 9) und für die verschiedenen Positionen des Minimums (20, 21, 22, 23),- Bestimmen (5) von Positionen der einzelnen Fluoreszenzmarker (8, 9) in der Probe (13) aus den für den jeweiligen Fluoreszenzmarker (8, 9) für die verschiedenen Positionen (20, 21, 22, 23) ...

Anordnung und Verfahren zur mehrkanaligen Fluoreszenzmessung in PCR-Proben

Anordnung zur mehrkanaligen Fluoreszenzmessung in PCR-Proben umfassend • einen Heizblock (1) mit Aufnahme (2) für PCR-Proben aufnehmende Kavitäten (41) einer Mikrotiterplatte (4), • eine transparente Folie (9) zum Verschließen der Kavitäten (41), • einen Messkopf (3), gegen den der Heizblock (1) hebbar und absenkbar angeordnet ist, wobei sich im Messkopf (3) eine Anregungslichteinheit (A), eine Detektionseinheit (D) sowie eine mechanische Einheit (M) befinden, dadurch gekennzeichnet, • dass die Detektionseinheit (D) eine drehbare Scheibe (26) mit Strahlteilermodulen (21; 31), einen Umlenkspiegel sowie einen Detektor (30) enthält wobei die Strahlteilermodule (21; 31) Filter einen dichroitischen Strahlteiler (27) eine Linse umfassen so dass Licht von der Anregungslichteinheit (A), nachdem es einen ersten Filter passiert hat, nacheinander in eine Vielzahl auf einer Kreisbahn angeordneter Lichtleiter (7) einkoppelbar ist, über die das Licht über vor den probenseitigen Enden der Lichtleiter ...

A FLUORIMETER

FILTER ASSEMBLY FOR FLUORESCENCE SPECTROSCOPY

Nucleic-acid-sequence determination device and nucleic-acid-sequence determination method

FLUORESCENCE SPECTROSCOPY METHOD

PROCEDURE AND MECHANISM FOR DETERMINING THE FERTILIZATION NEED IN GARDENS

OPTICAL TWO-WAVELENGTH FLUORESCENCE ANALYZER

OPTICAL EQUIPMENT IN PARTICULAR FOR THE MONITORING OF DNS POLYMERASEKETTENREAKTIONEN

INDICATOR SUBSTANCE FOR A MEASURING DEVICE FOR THE OPTICAL DETERMINATION OF INTERESTING PARAMETERS OF A SAMPLE AND A MEASURING PROCEDURE FOR IT

DETECTOR AND FILTERING DEVICE FOR ION CHANNELS

MICRO-FLUID ANALYSIS SYSTEMS AND - PROCEDURES WITH EXTREME HIGH THROUGHPUT

IMAGING SYSTEM WITH USE OF FLUORESCENCE.

PROCEDURE FOR THE PROOF OF REACTIONS OF MEANS COINCIDENCE ANALYSIS

PROCEDURE FOR THE COLLECTION OF FLUORESCENT MOLECULES OR OTHER PARTICLES OF MEANS PRODUCING FUNCTIONS

DETECTOR AND FILTERING DEVICE FOR ION CHANNELS

DIFFERENCE GEL ELECTROPHORESIS WITH SEVERAL ÜBEREINSTMMUNGSFARBSTOFFEN

UP-CONVERTING REPORTERS MOLECULE FOR BIOLOGICAL ONES AND OTHER TEST PROCEDURES USING SUGGESTION FOR LASER

Time-resolved laser-induced fluorescence spectroscopy systems and uses thereof

The invention provides systems for characterizing a biological sample by analyzing emission of fluorescent light from the biological sample upon excitation and methods for using the same. The system includes a laser source, collection fibers, a demultiplexer and an optical delay device.

Elimination of crosstalk in confocal epifluorescent microarray imaging systems

Ultra high throughput microfluidic analytical systems and methods

BIOASSAY READING SYSTEM

Device and method for detection of fluorescence labelled biological components

Method and system for detecting, classifying and identifying particles

A method and apparatus is disclosed for detecting, classifying and identifying airborne and non-airborne particles on an individual basis in substantially real time by directing a particle stream to react with optical reporters and markers and then exposing the stream to an excitation source such that individual particles have their multiple identifying characteristics detected.

Modular imaging device, module for this device, and method of implementation by this device

Systems and methods to perform assays for detecting or quantifying analytes within samples

Fluorescence-based detection methods and apparatus

Systems and methods for imaging using absorption

System and method for detecting and classifying biological particles

Ultra high throughput microfluidic analytical systems and methods

Method and apparatus for portable product authentication

System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents

The present disclosure relates to a method for analyzing tissue specimens. In one implementation the method involves obtaining a tissue sample and exposing the sample to one or more fluorophores as contrast agents to enhance contrast of subcellular compartments of the tissue sample. The tissue sample is illuminated by an ultraviolet (UV) light having a wavelength between about 200 nm to about 400 nm, with the wavelength being selected to result in penetration to only a specified depth below a surface of the tissue sample. Inter-image operations between images acquired under different imaging parameters allow for improvement of the image quality via removal of unwanted image components. A microscope may be used to image the tissue sample and provide the image to an image acquisition system that makes use of a camera. The image acquisition system may create a corresponding image that is transmitted to a display system for processing and display.

Method and apparatus for reading reporter labeled beads

Method and apparatus for sensing the presence of microbes

METHOD FOR ASSESSING BIOFILMS

An automated method for measuring the development of a biofilm on a plurality of surfaces using a confocal imaging system including: a) a radiation source system for forming a beam of electromagnetic radiation comprising one or more wavelengths; b) an optical system for directing and focusing said beam onto one or more planes of the object; c) a detection system for detecting electromagnetic radiation emitted from the object and producing image data; and d) a scanning system for scanning the object in a plurality of planes with the electromagnetic radiation, the method comprising the steps of: i) growing said biofilm on said plurality of surfaces; ii) detecting the presence of one or more fluorescent moieties within the biofilm by scanning the biofilm with electromagnetic radiation in a plurality of planes to produce a plurality of images; and iii) analysing said images by means of a data processing system under the control of computer software to determine the structure of the biofilm.

SYSTEM AND METHOD FOR DETECTING AND CLASSIFYING BIOLOGICAL PARTICLES

A continuous wave laser excites a biological particle. Detection channels are created to detect light scattered by the biological particle, and to detect any auto~fluorescence emitted by the biological particle. Additional channels can also detect light emitted by auto-fluorescence of the biological particle when simultaneously excited by light at harmonics of the laser's fundamental wavelength. The biological particle is identified using Mie scattering and auto~fluorescence. Ratio-metric calculations generated by calculating ratios of detected peak heights or integrated pulse values in the channels provides additional information for identifying and classifying the biological particle. A warning or alert can be provided if the identified biological particle is a particle of interest.

BIOCHEMICAL ASSAY DETECTION IN A LIQUID RECEPTACLE USING A FIBER OPTIC EXCITER

Biochemical assays that are performed in multi-well plates (11) and that utilize a system that supplies excitation light through an optical fiber (21, 22) that transmits light from an excitation light source (14) to the well (12). Emission light produced by the excitation is then collected by a collimating lens (31) and converted to a signal that is compiled for analysis. The optical fiber (21, 22) and collimating lens (31) can either be on the same side of the receptacle or on opposite sides, i.e., one above the other below. When the optical fiber and the collimating lens are both on the open side of the receptacle, they are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the mouth of the receptacle.

DETECTION DEVICE AND METHODS OF USE

An imaging system for exciting and measuring fluorescence on or in samples comprising fluorescent materials (e.g. fluorescent labels, dyes or pigments). In one embodiment, a device is used to detect fluorescent labels on nucleic acid. In a preferred embodiment, the device is configured such that fluorescent labels in a plurality of different DNA templates are simultaneously detected.

SERIAL-LINE-SCAN-ENCODED MULTI-COLOR FLUORESCENCE MICROSCOPY AND IMAGING FLOW CYTOMETRY

A system for performing high-speed, high-resolution imaging cytometry utilizes a line-scan sensor. A cell to be characterized is transported past a scan region. An optical system focuses an image of a portion of the scan region onto at least one linear light sensor, and repeated readings of light falling on the sensor are taken while a cell is transported though the scan region. The system may image cells directly, or may excite fluorescence in the cells and image the resulting light emitted from the cell by fluorescence. The system may provide a narrow band of illumination at the scan region. The system may include various filters and imaging optics that enable simultaneous multicolor fluorescence imaging cytometry. Multiple linear sensors may be provided, and images gathered by the individual sensors may be combined to construct an image having improved signal-to-noise characteristics.

OPTICAL SENSOR ASSEMBLY

The present invention provides systems and methods for measuring an analyte in a medium without exposing the medium to contamination. The systems and methods employ a novel combination of a small sensor device embedded in a Luer cap and capable of wirelessly transmitting data to a reading device.

MULTI-FUNCTION ANALYTIC DEVICES

The present disclosure provides devices, systems, methods for processing and/or analyzing a biological sample. An analytic device for processing and/or analyzing one or more biological samples may be electronically and/or physically configured or programed to activate one or more features/operations of the analytic device. The analytic device can be configured or programed by one or more instructions received from a cooperating electronic device or a remote server. The analytic device may comprise a moving carriage. The analytic device may be portable. The analytic device may receive instructions for performing an assay from a mobile electronic device external to a housing of the analytic device.

SENSOR SYSTEM

PC 7147 A sensor system for determining the pH or carbon dioxide concentration of a liquid medium comprising, in combination, a pH-insensitive fluorescent indicator and a pH-sensitive fluorescent indicator which act in concert or a single fluorescence indicator which emits fluorescent signals of different wavelengths in different carriers, which system(s) produce diverging signals, the ratio of which provides an accurate and stable determination of the parameter being measured. A method for determing pH and CO2 concentration is also disclosed.

APPARATUS FOR FLUORESCENCE SPECTROSCOPY

ANALYSIS AND TREATMENT OF BODY WEIGHT AND EATING DISORDERS

Proteins that are differentially expressed in body weight and eating disorders, such as obesity, are identified. Agents to treat such conditions may be identified by a screening method comprising: a) establishing a paradigm in which at least one protein is differentially expressed in relevant tissue from, or representative of, subjects having different levels of body weight or eating dysfunction; b) obtaining a sample of relevant tissue taken from, or representative of, a subject having body weight or eating disorders, who or which has been treated with the agent being screened; c) determining the presence, absence or degree of expression of the differentially expressed protein(s); and d) selecting or rejecting the agent according to the extend to which it changes the expression of the differentially expressed protein(s).

METHOD AND SYSTEM FOR DETECTING, CLASSIFYING AND IDENTIFYING PARTICLES

A method and apparatus is disclosed for detecting, classifying and identifying airborne and non-airborne particles on an individual basis in substantially real time by directing a particle stream to react with optical reporters and markers and then exposing the stream to an excitation source such that individual particles have their multiple identifying characteristics detected.

OPTICAL MICROSCOPY WITH PHOTOTRANSFORMABLE OPTICAL LABELS

First activation radiation is provided to a sample that includes phototransformable optical labels ("PTOLs") to activate a first subset of the PTOLs in the sample. First excitation radiation is provided to the first subset of PTOLs in the sample to excite at least some of the activated PTOLs, and radiation emitted from activated and excited PTOLs within the first subset of PTOLs is detecting with imaging optics. The first activation radiation is controlled such that the mean volume per activated PTOLs in the first subset is greater than or approximately equal to a diffraction-limited resolution volume ("DLRV") of the imaging optics.

SYSTEM, METHOD, AND COMPUTER SOFTWARE PRODUCT FOR LINKED WINDOW INTERFACES

Systems, methods, and computer program products are described for providing a graphical user interface (GUI) that may include a first openable window of image features constituting, for example, a pseudo-image of a scanned probe array. The image features each have one or more characteristics representing one or more hybridization reactions associated with a probe of the probe array. The GUI also has a second openable window including data features, each relating to one or more quantifications of one or more hybridization reactions associated with a probe of the probe array. This second window may be, for example, a scatter plot of hybridization intensities of probes to two or more labeled samples. The GUI further includes a third openable window including descriptive features such as rows of a spreadsheet. Each row may include descriptive elements associated with a probe. When a user selects a feature from any of the two or more windows, a corresponding feature in at least one other of ...

SPECTROMETER, IN PARTICULAR A REFLECTION SPECTROMETER

The invention relates to a reflection spectrometer provided with a probe to which the radiation of at least one radiation source can be transmitted by means of at least one radiation emission conductor-transmitter in such a way that said radiation source can be directed to or in an investigated object and which makes it possible to transmit a radiation, in particular fluorescent, reflected and/or diffused to or in an investigated object and/or emitted by said object to a radiation receiver that can be connected to an evaluation unit by means of at least one radiation emission conductor. The inventive reflection spectrometer is characterised in that it comprises a plurality of radiation sources whose radiation intensities are respectively adjustable, have a large emission range for a radiation source or for all radiation sources and are directly connected to a respective radiation emission conductor. The radiation receiver receives the entire spectrum of an incident radiation in the radiation ...

METHOD AND APPARATUS FOR SPECTROSCOPIC ANALYSIS, IMPLEMENTNG INFRARED AND FLUORESCENCE MULTICHANNEL PROCESSING OF SPECTRAL DATA

Procédé d'analyse d'au moins un échantillon, mettant en oeuvre une méthode d'analyse de données spectroscopiques basée sur un modèle statistique multivoies, comportant : a) l'éclairage dudit ou de chaque échantillon à analyser par une première source de lumière et par une deuxième source de lumière, ladite au moins une deuxième source de lumière étant distincte de ladite première source de lumière; b) l'acquisition de spectres de fluorescence dudit ou de chaque échantillon, lesdits spectres de fluorescence résultant de l'éclairage dudit ou de chaque échantillon par un ou plusieurs rayonnements lumineux émis par ladite première source de lumière; c) l'acquisition de spectres de transmittance et/ou de réflectance dudit ou de chaque échantillon, lesdits spectres de transmittance et/ou de réflectance résultant de l'éclairage dudit ou de chaque échantillon par un ou plusieurs rayonnements lumineux émis par ladite deuxième source de lumière; d) l'organisation desdits spectres de fluorescence ...

INTEGRATED DEVICE FOR DETECTING AND ANALYZING MOLECULES

System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device may include multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes a surface having a trench region recessed from a portion of the surface and an array of sample wells, disposed in the trench region. The integrated device also includes a waveguide configured to couple excitation energy to at least one sample well in the array and positioned at a first distance from a surface of the trench region and at a second distance from the surface in a region separate from the trench region. The first distance is smaller than the second distance. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device ...

SINGLE LIGHT SOURCE, TWO-OPTICAL CHANNEL SEQUENCING

Disclosed is a system for determining the nucleotide sequence of polynucleotides. The system can comprise a light source, such as a laser or a LED, configured to generate light at a predetermined wavelength. A detector of the system can detect fluorescent emissions at a first wavelength and a second wavelength. A processor of the system identify the nucleotide as a first type if no fluorescent emission is detected by the at least one detector; identify the nucleotide as a second type if a fluorescent emission at the first wavelength of light is detected by the at least one detector; identify the nucleotide as a third type if a fluorescent emission at the second wavelength of light is detected by the at least one detector; and identify the nucleotide as a fourth type if fluorescent emissions at the first wavelength and the second wavelength of light are detected by the at least one detector.

METHOD AND APPARATUS FOR PORTABLE PRODUCT AUTHENTICATION

A method and apparatus for on-site verification of product authentication and quality includes a microplate having a substrate with a light-emissive compound thereon. The substrate provides immobilization of the light-emissive compounds and provides a three-dimensional environment similar to free solution for reactions with the product sample to occur. The microplate may include any material having desired light reflective properties and a surface to retain the light-emissive compounds therein. A metered amount of light- emissive compound is placed on the microplate by any desired metering method, such as hand-metering by skilled technicians, automatic metering using robotic equipment, or printing using for example, piezoelectric dispensing technology. In this respect, the light-emissive compound is placed on a microplate, with the microplate. Once the light-emissive compound is applied to the substrate, the microplate may be sent to the test site where product testing is to be performed ...

METHOD FOR ENCODING MATERIALS WITH A LUMINESCENT TAG AND APPARATUS FOR READING SAME

A method, apparatus and system for analysing and authenticating a luminescent tag are provided wherein a luminescence is triggered in the tag and relative light intensities emitted thereby measured within at least two luminescence bands. The measured intensities are compared with at least one reference tag representative of relative intensities emitted by an authentic tag to determine whether the luminescent tag corresponds with any of the at least one reference tag. If the measured intensities match the representative intensities, the luminescent tag is authenticated. A computer program to be implemented by the apparatus and system is also disclosed. Further, a method and system for verifying the authenticity of one or plural objects are disclosed, wherein each of the objects is to be associated with a respective tag. The respective tags are coded to emit light, when triggered, within at least two luminescence bands at respective predetermined relative light intensities. Having access ...

TUNABLE EXCITATION AND/OR TUNABLE DETECTION MICROPLATE READER

FLUORESCENCE FILTERING SYSTEM AND METHOD FOR MOLECULAR IMAGING

An optical system is disclosed that can be used for fluorescence filtering for molecular imaging. In one preferred embodiment, a source subsystem is disclosed comprising a light source and a first set of filters designed to pass wavelengths of light in an absorption band of a fluorescent material. A detector subsystem is also disclosed comprising a light detector, imaging optics, a second set of filters designed to pass wavelengths of light in an emission band of the fluorescent material, and an aperture located at a front focal plane of the imaging optics. A telecentric space is created between the light detector and the imaging optics, such that axial rays from a plurality of field points emerge from the imaging optics parallel to each other and perpendicular to the second set of filters.

SYSTEMS AND METHODS FOR IMAGING USING ABSORPTION

The attenuation and other optical properties of a medium are exploited to measure a thickness of the medium between a sensor and a target surface. Disclosed herein are various mediums, arrangements of hardware, and processing techniques that can be used to capture these thickness measurements and obtain three-dimensional images of the target surface in a variety of imaging contexts. This includes general techniques for imaging interior/concave surfaces as well as exterior/convex surfaces, as well as specific adaptations of these techniques to imaging ear canals, human dentition, and so forth.

SYSTEMS AND METHODS TO PERFORM ASSAYS FOR DETECTING OR QUANTIFYING ANALYTES WITHIN SAMPLES

An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations in which discrete aspects of the assay are performed on fluid samples contained in sample vessels. The analyzer includes stations for automatically preparing a sample, incubating the sample, preforming an analyte isolation procedure, ascertaining the presence of a target analyte, and analyzing the amount of a target analyte. An automated receptacle transporting system moves the sample vessels from one station to the next. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte, and, in one embodiment, a method for real-time monitoring of the amplification process.

BIOSENSOR FOR CONFORMATION AND SECONDARY STRUCTURE ANALYSIS

The invention provides a biosensor for conformation and secondary structure analysis, notably for the direct non-invasive qualitative secondary structure analysis of a single selected protein within a complex mixture, as e.g. a body fluid, by vibrational spectroscopic methods. For the analysis it is not required that the selected substance be isolated, concentrated, or pretreated by a special preparative procedure.

METHODS AND DEVICES FOR XEROGEL BASED SENSORS

Chemical sensors today are deployed in massive volumes across multiple industries and yet at the same time they are subject to substantial research and development effort to establish new, faster, lower cost, more accurate, more sensitive chemical sensors. Such sensors and sensor arrays are being exploited across chemistry, biology, clinical biology, environmental science in civilian and military markets. Amongst the many sensor methodologies are xerogel substrates with two moieties, a receptor for molecular recognition of the analyte and a luminophore for signaling the recognition event. In order to fulfill the requirements for low cost there is a requirement for electronic excitation / read circuits that can support architectures with optical source ¨ N sensors ¨ X filters ¨ M detectors, where M >= N and X = N¦M . According to embodiments of the invention electronic excitation / read circuits for phase based luminophore sensors are presented that are compatible with single CMOS chip implementation ...

METHOD AND SYSTEM FOR PORTABLE CELL DETECTION AND ANALYSIS USING MICROFLUIDIC TECHNOLOGY

The present invention is a method and a system of cell detection and analysis. The present invention may incorporate at least an optical source, a fluidic chip and a detection module. Cells may be caused to flow within the fluidic chip and specifically past a detection window section accessible by the optical source. The flowing cells may be identified and/or analyzed. The detection module may specifically count the cells of interest as they flow past the detection window section of the chip. The detection module may further be operable to generate or otherwise capture images of the cells as they flow past the window and to use these images collectively for the purpose of analyzing the cells. The present invention may be portable and operable in remote locations.

MAN-PORTABLE DEVICE FOR DETECTING HAZARDOUS MATERIAL

A man portable device for detecting the presence of hazardous material includes a pulsed or time-modulated light source and an objective scannable across the surface of a sample for projecting light from the light source onto a succession of spots on the surface of the sample. A spectrometer performs a spectral analysis of the induced fluorescence to create a first dataset defining a first vector as a function of wavelength. A time domain detector for measuring the time decay of the induced fluorescence collected simultaneously creates a second dataset defining a second vector as a function of time. A computer identifies hazardous material by performing independent multivariate analysis on the first and second vectors as the objective is scanned across the sample surface based on fluorescent signal models for hazardous materials in the spectral and time domains.

SYSTEM AND METHODS FOR DETECTING MULTIPLE OPTICAL SIGNALS

An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations in which discrete aspects of the assay are performed on fluid samples contained in sample vessels. The analyzer includes stations for automatically preparing a sample, incubating the sample, preforming an analyte isolation procedure, ascertaining the presence of a target analyte, and analyzing the amount of a target analyte. An automated receptacle transporting system moves the sample vessels from one station to the next. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte, and, in one embodiment, a method for real-time monitoring of the amplification process.

Verfahren und Vorrichtung zum Aufzeichnen von Informationen unter Verwendung eines photolumineszenten Materials

Method and device for identifying bodies containing or carrying a luminous material

The aim is to provide an improved device and a method for identifying objects or bodies by checking the distribution of intensity and wavelength in the emission spectrum. A source of exciting radiation serves to direct exciting radiation onto the body located in a test station. A plurality of light pipes (24, 25, 26) simultaneously receive the luminous radiation emitted by the luminous material and feed the luminous radiation to photodetectors (18, 19, 20). Assigned to each light pipe (24, 25, 26) is an optical filter (21, 22, 23) which passes radiation of a specific wavelength or of a specific wavelength region. A circuit receives the electric output signals derived from the photodetectors and changes the signal level of the photodetectors by attenuation or amplification. The changed signal levels are compared with the signal level derived from another photodetector. It is indicated whether the level ratio is in a specific desired range. ...

Apparatus for inspecting samples of cells by means of fluorescence.

Die Erfindung betrifft einen Zellanalyse-Apparat, bestehend aus a. einem Probenträger (10), der eine Zellprobe enthält, welche mit mindestens einem fluoreszierenden Agens behandelt wurde; b. einem Probenmess-Mittel, bestehend aus (i) mindestens einem Probenbeleuchtungs-Mittel (101, 102), wobei das mindestens eine Probenbeleuchtungs-Mittel (101, 102) aus einer Lichtquelle besteht, um die Probe zu beleuchten; (ii) einem Detektions-Mittel (103), bestehend aus einer ferngesteuerten Fokussierlinse (15), die darauf ausgelegt ist, die fluoreszente Strahlung, die von der Probe emittiert wird, zu empfangen; und d. einem Kontroll- und Datenanalyse-Mittel (1), das darauf ausgelegt ist, die funktionellen Operationen des Probenmess-Mittels zu kontrollieren und Daten zu empfangen, analysieren und Resultate zu präsentieren, wobei das Kontroll- und Datenanalyse-Mittel (1) getrennt von der Probe und vom Probenmess-Mittel vorliegt. Der Apparat ist leicht und transportierbar.

METHOD AND DEVICE FOR HIGH-RESOLUTION SPECTROMETRY IN WELL

LOGOMETRIChESKIE FLUORESCENCE VISUALIZATION METHODS

METHOD OF EXPRESS DETECTION OF NARCOTICS IN CONFISCATED SAMPLES SUBSTANCES

СПЕКТРОМЕТР СО СВЕТОДИОДНОЙ МАТРИЦЕЙ

Предлагается устройство (110) для определения по меньшей мере одного оптического свойства образца (112). Устройство (110) включает в себя настраиваемый источник возбуждающего света (114, 410) для подачи луча возбуждающего света (122) на образец (112). Устройство (110) включает в себя также детектор (128, 130, 312) для определения исходящего от образца детектируемого излучения (132, 136, 314). Источник возбуждающего света (114, 410) включает светодиодную матрицу, выполненную, по меньшей мере, частично в виде монолитной светодиодной матрицы (114). Монолитная светодиодная матрица (114) состоит по меньшей мере из трех светодиодов с различным спектром излучения.

СПОСОБ ОБРАБОТКИ НАБОРОВ ФЛУОРЕСЦЕНТНЫХ ИЗОБРАЖЕНИЙ И УСТРОЙСТВО ДЛЯ ЕГО ОСУЩЕСТВЛЕНИЯ

В заявке описаны способ и устройство, предназначенные для обработки флуоресцентных частичных изображений, представляющих одну и ту же область объекта, и работающие независимо от постороннего света. С помощью камеры (10) создают два частичных изображения объекта в красном и зеленом цветах. Из этих двух частичных изображений попиксельно формируют изображение-частное, и для этого изображения-частного определяют, с какой частотой встречаются точки изображения, имеющие заданное отношение красного и зеленого цветов. Для полученной таким образом кривой распределения определяют среднее значение и ширину. Эти две конечные величины кривой распределения используют для вычисления порогового значения. Затем с применением этого порогового значения модифицируют изображение-частное таким образом, чтобы повысить контраст в отношении интересующих деталей.

СПОСОБ И УСТРОЙСТВО ДЛЯ СПЕКТРОМЕТРИИ ВЫСОКОГО РАЗРЕШЕНИЯ В СКВАЖИНЕ

В изобретении предлагаются способ и устройство, предусматривающие использование в скважине интерференционных светофильтров (109), имеющих возможность вращения, качания и шагового поворота для изменения угла падения света (102), проходящего через эти светофильтры после прохождения через анализируемую пробу (105). При наклоне каждого светофильтра цвет или длина волны света, проходящего через этот светофильтр, изменяется. Для изоляции фотодиода каждого светофильтра между светофильтрами расположены черные пластины-экраны. Предлагаемый спектрометр пригоден для использования с кабельным опробователем пластов для проведения дополнительного анализа и контроля чистоты проб. Настоящее изобретение также применимо в составе оборудования для получения скважинной информации в процессе бурения. Предлагаемое устройство для спектрометрии высокого разрешения позволяет определять процентное содержание в нефти ароматических углеводородов, олефинов и предельных углеводородов с целью определения соотношения ...

МЕТОД ЭКСПРЕССНОГО ОБНАРУЖЕНИЯ НАРКОТИКОВ В КОНФИСКОВАННЫХ ПРОБАХ ВЕЩЕСТВ

Данное изобретение связано с обнаружением наркотиков в изъятом образце. Предлагаемый способ детектирования героина и морфия основан на технологии спектральных флуоресцентных образов (СФО). Способ включает приготовление из уличного образца жидкой пробы для анализа, разделение жидкой пробы на две аликвотные (кратные) пробы, первая из которых является контрольной пробой, а вторая является основной пробой, добавление соляной кислоты, а затем гидроксида натрия в контрольную пробу до измерения, выполнение измерения СФО контрольной пробы, обнаружение присутствия специфического спектрального образца морфия в измеряемом СФО контрольной пробы и фиксацию значения интенсивности СФО в особой спектральной точке. Величину интенсивности СФО контрольной пробы в особой спектральной точке рассматривают как контрольную величину. Далее добавляют гидроокись натрия в основную пробу до измерения. После истечения срока, предусмотренного для гидролиза основной пробы, в нее добавляют соляную кислоту, вводят основную ...

Instrument for monitoring polymerase chain reaction of DNA

Tumor site recognition device and method for identifying

Determining the quantity of nucleic acid, particularly DNA or RNA in a sample comprises adding a fluorophore to the sample, measuring fluorescence intensities in response to luminous stimulations and removing the nucleic acids

L'invention concerne un procédé de détermination de la quantité d'acide nucléique présente dans un échantillon, dans lequel : - on ajoute un fluorophore à l'échantillon, - on mesure des intensités de fluorescence émises par le fluorophore à au moins trois longueurs d'onde d'émission en réponse à des stimulations lumineuses à au moins trois longueurs d'onde d'excitation respectivement, et - on déduit des intensités de fluorescence mesurées la quantité d'acide nucléique présente dans l'échantillon.

PROCESS AND MOLECULAR SYSTEM Of ANALYSIS HAVE COMMUTATION OF MEASUREMENTS Of IMAGERY AND SPECTROSCOPY

L'invention se rapporte à un système d'analyse moléculaire (1), comprenant un dispositif de microscopie confocale (2) muni de moyens d'illumination (20) agencés pour exciter les molécules, et un dispositif d'imageries corrélées (7) de spectre et de temps de vie de la fluorescence émise par les molécules excitées. Ce système comprend en outre un dispositif de spectroscopie par corrélation (5;6) de la fluorescence émise par les molécules excitées, apte à commuter avec le dispositif d'imageries corrélées (7) de sorte à réaliser de manière simultanée des mesures d'imageries corrélées et de spectroscopie, ainsi qu'un dispositif de contrôle (8) agencé de sorte que les données issues de ce dispositif d'imageries corrélées (7) participent à l'ajustement des données issues du dispositif de spectroscopie par corrélation (5;6). L'invention se rapporte également à un procédé d'analyse moléculaire mettant en œuvre un tel système (1).

DEVICE AND PROCESS Of MICROSCOPIC ANALYSIS MULTIPARAMETRIQUE Of ELEMENTS

Process to record and locate information

APPARATUS Of ANALYSIS OF PLANTS ON the GROUND, PROCESS OF FOLLOW-UP OR CARTOGRAPHY OF the STATE OR the EVOLUTION Of a CULTURE AND PROCESS OF MANAGEMENT Of a TREATMENT OF PLANTS

PROCESS FOR DETERMINING THE TIME OF DECLINE OF A LUMINESCENCE SIGNAL

PROCESS OF CHARACTERIZATION Of an AGROALIMENTARY PRODUCT AND APPARATUS FOR the IMPLEMENTATION Of SUCH a PROCESS.

Procédé de caractérisation d'un échantillon d'un produit agroalimentaire, en particulier destiné à déterminer la naturalité, la fraîcheur, l'authenticité d'un tel produit, et/ou sa conformité à un produit cible. Le procédé de l'invention est caractérisé en ce qu'il comporte l'acquisition d'une pluralité de spectres de fluorescence naturelle de l'échantillon, l'application à ces spectres d'un procédé d'analyse multivariée ou multivoies fournissant un nombre limité N de variables représentatives dudit ou de chaque échantillon, de manière à permettre sa représentation par un point (PE) dans un espace à N dimensions ; le calcul d'une distance (D) entre ce point représentant ledit ou chaque échantillon et une cible (Cl) représentant un ou plusieurs échantillons de référence ; et la détermination d'une caractéristique dudit ou de chaque échantillon en fonction de ladite distance (D).

PORTABLE DEVICE FOR MONITORING AN ALCOHOLIC BEVERAGE THROUGH A CONTAINER, SYSTEM AND METHOD

Optical system for multi-channel fluorescence measurement of microfluidic chip and multi-channel fluorescence sample analyzer

METHOD AND APPARATUS FOR MONITORING OIL OXIDATION IN REAL TIME BY MEASURING FLUORESCENCE

Use of fccs for the analysis of interaction parameters in an in vivo-like environment

The present invention relates to the determination of interaction parameters of at least two analytes in cellular lysates, wherein at least one competitive agent is optionally further present.

Fluorescent Estimating Apparatus, Fluorescent Estimating Method, and Fluorescent Measuring Apparatus

This invention provides a technique for efficiently estimating spectral radiance factors of fluorescence of a sample. To this end, for a sample which contains a fluorescent substance, first spectral reflectance data and second spectral reflectance data are obtained by making two different variable-angle measurements using a general colorimeter under a white light source. Ratios n of the first spectral reflectance data to the second spectral reflectance data in a long-wavelength spectrum range outside a fluorescent spectrum range of the sample are calculated. Then, difference data between spectral reflectance data as results of multiplying the second spectral reflectance data by the ratios n, and the first spectral reflectance data are calculated. The difference data are divided by values obtained by subtracting 1 from the ratios n, thereby calculating spectral reflectances of fluorescence of the sample, that is, spectral radiance factors.

Excitation light source assembly

An excitation light source assembly includes a housing defining a central opening therein and a plurality of lamp receptacles surrounding the central opening. The housing is mountable to a support structure having a camera mounted thereto so that a field of view of the camera is substantially unobstructed by the housing. A light source is positioned within each of the plurality of lamp receptacles. A diffuser is positioned adjacent the light source in each of the plurality of lamp receptacles so that each of the diffusers diffuses light produced by each of the light sources. A control system operatively connected to each of the light sources operates selected ones of the light sources to provide a desired excitation illumination to an object within the field of view of the camera.

Fluorescence detecting optical system and multi-channel fluorescence detection apparatus including the same

A fluorescence detection optical system detects fluorescence beams with two or more different wavelengths and maintains a focal position through an automatic focusing function. A multi-channel fluorescence detection apparatus includes the fluorescence detection optical system. The fluorescence detection optical system includes an automatic focusing unit which receives light reflected off a microfluidic device and determines a focal point by using an astigmatic method or a knife edge method, and an actuator which adjusts a position of an objective lens according to control of the automatic focusing unit. In addition, the fluorescence detection optical system may include a plurality of dual band pass filters, dichroic devices, etc., which provide light beams emitted from at least two light sources and transfer fluorescence generated from the microfluidic device to a photodetector.

Explosives Detection Substrate and Methods of Using the Same

Provided herein are explosives detection substrates which include an electrospun (electro)sprayed and/or dry spun aromatic polymer, such as polystyrene, and a small molecule fluorophore. Methods for detecting an explosive material using such substrates are also provided.

Optical instrument comprising multi-notch beam splitter

An instrument is provided that can monitor nucleic acid sequence amplification reactions, for example, PCR amplification of DNA and DNA fragments. The instrument includes a multi-notch filter disposed along one or both of an excitation beam path and an emission beam path. Methods are also provided for monitoring nucleic acid sequence amplifications using an instrument that includes a multi-notch filter disposed along a beam path.

WIDE-FIELD IMAGING USING NITROGEN VACANCIES

Nitrogen vacancies in bulk diamonds and nanodiamonds can be used to sense temperature, pressure, electromagnetic fields, and pH. Unfortunately, conventional sensing techniques use gated detection and confocal imaging, limiting the measurement sensitivity and precluding wide-field imaging. Conversely, the present sensing techniques do not require gated detection or confocal imaging and can therefore be used to image temperature, pressure, electromagnetic fields, and pH over wide fields of view. In some cases, wide-field imaging supports spatial localization of the NVs to precisions at or below the diffraction limit. Moreover, the measurement range can extend over extremely wide dynamic range at very high sensitivity. 1. A method of imaging at least one of an electric field , magnetic field , temperature , pressure , or strain applied to a color center , the method comprising:(A) applying a magnetic field from a microwave source to the color center so as to manipulate an electron spin state of the color center;(B) irradiating the color center with an optical pulse from a light source so as to excite the color center from a first energy level to a second energy level and to induce emission of fluorescence from the color center, the fluorescence representative of the at least one of the electric field, magnetic field, temperature, pressure, or strain applied to the color center; and(C) imaging, with a wide-field imaging system, the fluorescence emitted by the color center onto a detector array.2. The method of claim 1 , further comprising claim 1 , before (A):disposing the color center on a surface of an inorganic material; andexposing the inorganic material to the at least one of the electric field, magnetic field, temperature, pressure, or strain.3. The method of claim 1 , wherein the color center is disposed within a nanodiamond claim 1 , and further comprising claim 1 , before (A):functionalizing a surface of the nanodiamond; anddisposing the nanodiamond within ...

TIME-RESOLVED LASER-INDUCED FLUORESCENCE SPECTROSCOPY SYSTEMS AND USES THEREOF

The invention provides systems for characterizing a biological sample by analyzing emission of fluorescent light from the biological sample upon excitation and methods for using the same. The system includes a laser source, collection fibers, a demultiplexer and an optical delay device. All references cited herein are incorporated by reference in their entirety as though fully set forth. Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. 1. A system for characterizing a biological sample by analyzing emission of fluorescent light from the biological sample upon excitation comprising:(i) a laser source connected to a biological sample via excitation fibers (ExF), wherein the laser is configured to radiate the biological sample with a laser pulse at a predetermined wavelength to cause the biological to produce a responsive fluorescence signal;(ii) collection fibers (CF), wherein the CF collect the fluorescence signal from the sample, and relays the signal to a demultiplexer;(iii) a demultiplexer comprising wavelength splitting filters so as to split the signal from the CF at pre-determined wavelengths to obtain spectral bands; and(iv) an optical delay device.2. The system of claim 1 , further comprising a photomultiplier tube comprising a preamplifier so as to amplify the signal after the signal has passed through the photomultiplier tube before the signal is digitized.3. The system of claim 2 , further comprising a digitizer so as to digitize the signal received from the photomultiplier tube and a computer system to process and display the signal.4. The system of claim 1 , wherein the optical delay device is adapted to couple the spectral bands from the demultiplexer into the delay device claim 1 , allow the spectral bands to travel through the delay device and introduce a controlled time delay as the spectral bands travel through delay ...

FLUORESCENCE OBSERVATION METHOD AND FLUORESCENCE OBSERVATION APPARATUS

A fluorescence observation method of the present invention for detecting plural types of fluorescence emitted from two or more kinds of fluorescent molecules includes: subjecting each of the two or more kinds of fluorescent molecules to multi-photon excitation by exciting light having an exciting wavelength equal to or shorter than 700 nm in a visible region, to generate fluorescence upon making use of an absorption wavelength band in a deep ultraviolet region of each of the two or more kinds of fluorescent molecules; and simultaneously detecting plural types of fluorescence generated on a shorter-wavelength side or on both of the shorter-wavelength side and a longer-wavelength side of the exciting wavelength of the exciting light. 1. A fluorescence detection method for detecting plural types of fluorescence emitted from two or more kinds of fluorescent molecules , comprisingsubjecting each of the two or more kinds of fluorescent molecules to multi-photon excitation by exciting light having an exciting wavelength equal to or shorter than 700 nm in a visible region, to generate fluorescence upon making use of an absorption wavelength band in a deep ultraviolet region of each of the two or more kinds of fluorescent molecules, andsimultaneously detecting plural types of fluorescence generated on a shorter-wavelength side or on both of the shorter-wavelength side and a longer-wavelength side of the exciting wavelength of the exciting light.2. The fluorescence detection method according to claim 1 , wherein each of the two or more kinds of fluorescent molecules used for a fluorescence detection target has absorption wavelength bands in the deep ultraviolet region and in the visible region.3. The fluorescence detection method according to claim 1 , wherein the exciting light is an ultrashort-pulsed laser beam.4. The fluorescence detection method according to claim 1 , wherein only fluorescence generated on the shorter-wavelength side of the exciting wavelength is detected ...

System and method for improving calibration transfer between multiple raman analyzer installations

A method for harmonizing the responses of a plurality of Raman analyzers includes steps of calibrating an intensity axis response of a spectrometer to a reference light source and measuring a laser wavelength of a laser using the spectrometer. The method also includes steps of measuring a fluorescence spectrum induced by the laser at the laser wavelength of a plurality of standard reference material samples using the spectrometer, measuring a temperature of each standard reference material sample while measuring the fluorescence spectrum, and correcting the fluorescence spectrum of each standard reference material sample based on the respective temperature. The method further includes steps of deploying each standard reference material sample in one of a plurality of field calibrator devices and calibrating the intensity axis of one of the Raman analyzers using one of the field calibrator devices and the corrected fluorescence spectrum of the respective standard reference material sample.

Biosensor for Conformation and Secondary Structure Analysis

Provided herein is a biosensor for conformation and secondary structure analysis, notably for the direct non-invasive qualitative secondary structure analysis of a single selected protein within a complex mixture, as e.g. a body fluid, by vibrational spectroscopic methods. For the analysis it is not required that the selected substance be isolated, concentrated, or pretreated by a special preparative procedure. 1. An infrared sensor element for the direct analysis of the quantity and secondary structure of a candidate biomarker protein undergoing conformational transitions associated with disease progression , comprising a germanium internal reflection element of a trapezoid or parallelogram shape and transparent in the infrared spectrum with sufficient signal to noise ratio to detect an amide I band , and at least one receptor for the biomarker protein comprising an antibody capable of specific and conformationally independent binding to the candidate biomarker protein , the receptor directly grafted to at least one surface of said internal germanium reflection element by silanization with short silane linkers or by thiolation with short thiol linkers , reacting freely accessible amine groups of said at least one receptor with amine-reactive groups on the short silane/thiol linkers , and blocking remaining amine-reactive groups on the short silane/thiol linkers with a blocking substance not cross-reacting with the candidate biomarker protein.2. The infrared sensor element of claim 1 , wherein the element is further suitable for the parallel analysis by another optical method including detection of fluorescence at different wavelengths.3. The infrared sensor element of claim 1 , wherein the internal reflection element(i) is a germanium monocrystal; monocrystal; and/or(ii) allows for more than one passages of the infrared light through the reflection element.4. The infrared sensor element of claim 1 , wherein the silane and thiol linkers include homogenous silane and ...

MULTICOLOR FLUORESCENCE ANALYSIS DEVICE

A multicolor fluorescence analysis device is for detecting fluorescence emitted, as a result of excitation light irradiation, from a plurality of types of fluorophores included in a sample s, and is provided with an irradiation optical unit for irradiating light emitted from a light source onto a sample s as excitation light, a fluorescence condensation unit having a fluorescence filter that transmits light emitted from the sample s and transmits light of transmission wavelength bands different from the excitation wavelength bands, and a two-dimensional detector that has a plurality of types of transmission filters for transmitting prescribed wavelengths of light and detects the intensity of the light of the prescribed wavelength for each transmission filter and the light emitted from at least two fluorophores from among the plurality of types of fluorophores is detected simultaneously and the fluorophore types are identified accordingly. 1. A multicolor fluorescence analysis device for detecting fluorescence emitted from a plurality of types of fluorescent dyes having different fluorescence wavelengths contained in a sample by irradiation with excitation light , the multicolor fluorescence analysis device comprising:a light source for excitation;an irradiation optical unit having an excitation filter that transmits light in a plurality of different excitation wavelength bands and irradiating the sample with light emitted from the light source as the excitation light through the excitation filter;a fluorescence condensation unit having a fluorescence filter that transmits at least a portion of the fluorescence emitted from the sample by the irradiation of the excitation light and transmits light in a plurality of transmission wavelength bands not including the excitation wavelength band; anda two-dimensional detector having a plurality of types of transmission filters that transmit light having a predetermined wavelength out of the light transmitted by the ...

OPTICAL MODULE FOR MULTI-WAVELENGTH FLUORESCENCE DETECTION

An optical module for multi-wavelength fluorescence detection includes an excitation light source component having at least one excitation light channel and including a first dichroic mirror and at least one light source for generating irradiation light; a color filter component for forming multiple fluorescence transmission paths; and a fluorescence receiving component having multiple fluorescence receiving channels. The first dichroic mirror is arranged at an irradiation light path of respective light sources. The color filter component is arranged at a transmission light path of the first dichroic mirror and includes at least one second dichroic mirror for receiving fluorescence signals. The fluorescence receiving component includes a plurality of optical elements, which are respectively arranged at a reflection and/or transmission light path of the second dichroic mirror. The application enables the simultaneous and instantaneous detection of multi-wavelength fluorescence. 1. An optical module for multi-wavelength fluorescence detection , comprising:an excitation light source component having at least one excitation light channel and comprising a first dichroic mirror and at least one light source for generating irradiation light, the first dichroic mirror being arranged at an irradiation light path of respective light sources;a color filter component for forming multiple fluorescence transmission paths, the color filter component being arranged at a transmission light path of the first dichroic mirror and comprises at least one second dichroic mirror for receiving a fluorescence signal; anda fluorescence receiving component having multiple fluorescence receiving channels and comprising a plurality of optical elements, each optical element being arranged at a reflection light path and/or a transmission light path of the second dichroic mirror.2. The optical module of claim 1 , wherein the excitation light source component further comprises a forth dichroic ...

Measuring apparatus and fluorescence measuring method

A low-noise fluorescence image and a low-noise luminescence image are acquired even if a luminescent substance having a high luminescence level is used. Provided is a measuring apparatus including an illumination optical system that radiates excitation light coming from an excitation light source onto a specimen A, an image acquisition portion that acquires an image by measuring light generated at the specimen, and an image processing portion that, based on a first image, which is acquired by the image acquisition portion without radiating the excitation light, and a second image, which is acquired by the image acquisition portion while radiating the excitation light, generates a fluorescence image by removing a luminescence component from the second image.

Method for analysing a sample comprising at least a first and a second scale inhibitor

The invention relates to a method for analysing a sample comprising at least a first and a second scale inhibitor, which scale inhibitors are synthetic organic compounds comprising at least one ionised group. The method comprises optionally diluting and/or purifying the sample, and allowing the sample interact with a reagent comprising lanthanide(III) ion. The sample is excited at a first excitation wavelength and a sample signal deriving from the lanthanide(III) ion is detected at a signal wavelength by using time-resolved luminescence measurement. The total concentration of the first and the second scale inhibitor is determined by using the detected sample signal, and the concentration of the first scale inhibitor in the sample is determined. The concentration of the second scale inhibitor is determined mathematically by using the obtained results for the total concentration and for the first scale inhibitor concentration. 1. Method for analysing a sample comprising at least a first and a second scale inhibitor , which scale inhibitors are synthetic organic compounds comprising at least one ionised group , the method comprisingoptionally diluting and/or purifying the sample,allowing the sample interact with a reagent comprising lanthanide(III) ion,exciting the sample at a first excitation wavelength and detecting a sample signal deriving from the lanthanide(III) ion at a signal wavelength by using time-resolved luminescence measurement,determining the total concentration of the first and the second scale inhibitor by using the detected sample signal,determining the concentration of the first scale inhibitor in the sample,determining the concentration of the second scale inhibitor mathematically by using the obtained results for the total concentration and for the first scale inhibitor concentration.2. Method according to claim 1 , characterised in determining the concentration of the first scale inhibitor by exciting the sample at a second excitation wavelength ...

Spectral imaging apparatus and methods

An imaging system images a sample across one or more wavelengths. A light source illuminates a sample with one or more wave-lengths of light, and an image sensor detects light from the illuminated sample. A linear variable long pass filter is positioned to filter light reflected from the sample to pass to the image sensor multiple different wave-length bands having different cut-off wavelengths. Wavelengths of light on one side of the cut-off wavelength are blocked and wavelengths of light on the other side of the cut-off wavelength are passed as multiple different long pass wavelength bands for detection by the image sensor. The image sensor detects light for each of the multiple different long pass wavelength bands from the sample. Data processing circuitry converts the detected light for the multiple different long pass wavelength bands for the sample into corresponding different long pass wavelength band data sets for the sample.

HIGH-THROUGHPUT HYPERSPECTRAL IMAGING SYSTEMS

High-throughput hyperspectral imaging systems are provided. According to an aspect of the invention, a system includes an excitation light source; an objective that is configured to image excitation light onto the sample, such that the excitation light causes the sample to emit fluorescence light; a channel separator that is configured to separate the fluorescence light into a plurality of spatially dispersed spectral channels; and a sensor. The excitation light source includes a light source and a plurality of lenslet arrays. Each of the lenslet arrays is configured to receive light from the light source and to generate a pattern of light, and the patterns of light generated by the lenslet arrays are combined to form the excitation light. The objective is configured to simultaneously image each of the patterns of light to form a plurality of parallel lines or an array of circular spots at different depths of the sample. 1. A system comprising:an excitation light source that is configured to emit excitation light;an objective that is configured to receive the excitation light from the excitation light source and image the excitation light onto a sample, such that the excitation light causes the sample to emit fluorescence light;a channel separator that is configured to receive the fluorescence light from the sample and separate the fluorescence light into a plurality of spatially dispersed spectral channels; anda sensor that is configured to receive the plurality of spatially dispersed spectral channels from the channel separator, a plurality of light sources, wherein each of the plurality of light sources emits light having a different wavelength;', 'a dichroic array that is configured to combine light from each of the plurality of light sources; and', 'a first lenslet array, wherein the first lenslet array is configured to receive light from the dichroic array and to generate a plurality of patterns of light corresponding to the plurality of light sources;, ' ...

Microfluidic system for high-throughput, droplet-based single molecule analysis with low reagent consumption

A microfluidic device for a confocal fluorescence detection system has an input channel defined by a body of the microfluidic device, a sample concentration section defined by the body of the microfluidic device and in fluid connection with the input channel, a mixing section defined by the body of the microfluidic device and in fluid connection with the concentration section, and a detection region that is at least partially transparent to illumination light of the confocal fluorescence detection system and at least partially transparent to fluorescent light when emitted from a sample under observation as the sample flows through the detection region.

METHOD AND SYSTEM FOR ITEM AUTHENTICATION AND CUSTOMIZATION

A method of illumating an item is disclosed. The method includes applying adhesive to the item, interspersing a taggant in the adhesive, illuminating the item with an excitation signal, sensing luminescence emitted by the taggant in response to illumination by the excitation signal, and determining the authenticity of the item based on the sensed emitted luminescence. The item can include any item benefited by authentication, and can include a postage stamp. A method of customizing an item is disclosed. This can include the steps of preparing a substrate, applying a security feature to the substrate, printing non-customized information on the substrate, receiving image information, and printing the image information on the substrate. 1. A method of making an authenticatable label comprising:applying a printing to a first side of a substrate;applying an adhesive to the second side of the substrate;applying a taggant to a first side of the backing, wherein the taggant emits luminescence in response to illumination by an excitation signal; andattaching the substrate to the backing, wherein following attaching the substrate to the backing, at least a portion of the taggant migrates into the adhesive.2. The method of wherein the taggant is applied to the first side of the backing in a pattern comprising a pre-determined computer readable image which is readable upon excitation of the taggant from an excitation signal.3. The method of wherein the pattern is a barcode.4. The method of claim 2 , wherein the taggant is applied by printing the taggant onto the first side of the backing.5. The method of wherein applying the taggant to the first side of the backing comprises applying the taggant with a substance that dissolves the taggant into the adhesive during contact with the adhesive.6. The method of further comprising receiving an image from a user claim 1 , and wherein applying the taggant to the first side of the backing comprises applying the taggant in a pattern ...

Multi-Channel Optical Measurement Instrument

A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle. 1. A detector for detecting optical emissions of two or more different wavelengths or ranges of wavelengths from a sample , wherein emissions of two or more different wavelengths are indicative of the presence , amount , or state of two or more analytes of interest in the sample , the detector comprising: a light emitting element adapted to emit excitation light; and', 'excitation optical elements defining an excitation optical path having an excitation optic axis, the excitation optical elements being constructed and arranged to transmit at least a portion of the light emitted by the light-emitting element having the prescribed excitation wavelength or range of excitation wavelengths toward the sample;, 'two or more excitation channels fixed with respect to the sample and each other, wherein each excitation channel is adapted to direct an excitation signal of a different prescribed excitation wavelength or range of excitation ...

PHOTONIC SUPERLATTICE-BASED DEVICES AND COMPOSITIONS FOR USE IN LUMINESCENT IMAGING, AND METHODS OF USING THE SAME

Under one aspect, a device is provided for use in luminescent imaging. The device can include a photonic superlattice including a first material, the first material having a first refractive index. The first material can include first and second major surfaces and first and second pluralities of features defined through at least one of the first and second major surfaces, the features of the first plurality differing in at least one characteristic from the features of the second plurality. The photonic superlattice can support propagation of a first wavelength and a second wavelength approximately at a first angle out of the photonic superlattice, the first and second wavelengths being separated from one another by a first non-propagating wavelength that does not selectively propagate at the first angle out of the photonic superlattice. 1. A device comprising:a photonic superlattice comprising a surface having wells of different sizes, the photonic superlattice supporting propagation of a first wavelength and a second wavelength, the photonic superlattice inhibiting propagation of a third wavelength between the first and second wavelengths; andan optical component facing the surface, the optical component receiving first luminescence at the first wavelength at an angle, the optical component receiving second luminescence at the second wavelength at approximately the angle.2. The device of claim 1 , wherein the angle is approximately normal to the surface.3. The device of claim 1 , wherein the photonic superlattice comprises a first material that comprises the wells claim 1 , the device further comprising a second material in contact with the first material.4. The device of claim 3 , wherein the second material has a higher refractive index than the first material.5. The device of claim 3 , further comprising a third material in contact with at least the second material claim 3 , the third material comprising first and second luminophores claim 3 , the first ...

Methods for fluorescence data correction

Method of processing real-time PCR data, comprising: c) receiving a plurality of fluorescence melting curve data of real time PCR-experiments performed by a real-time PCR device with at least two fluorescence channels, and configured to perform the following steps multiple times, while increasing a temperature: i) at first moments in time measuring a first temperature value and a first radiation value corresponding to a first fluorescence channel; ii) at second moments in time measuring a second temperature value and a second radiation value corresponding to a second fluorescence channel; d) storing the plurality of temperature values and radiation values; e) determining a plurality of time-shifted second radiation values by linearly interpolating between two measured second radiation values, using weighting factors defined by the measured temperature values; f) after performing step e), calculating color corrected first radiation values, and color corrected second radiation values.

FLUORESCENT IMAGE ANALYZER, ANALYZING METHOD, AND PRETREATMENT EVALUATION METHOD

A fluorescence image analyzer, analyzing method, and pretreatment evaluation method capable of determining with high accuracy whether a sample is positive or negative are provided. A pretreatment part performs pretreatment including a step of labeling a target site with a fluorescent dye to prepare a sample. A fluorescence image analyzer measures and analyzes the sample. The fluorescent image analyzer includes light sources to irradiate light on the sample, imaging part to capture the fluorescent light given off from the sample irradiated by light, and processing part for processing the fluorescence image captured by the imaging part. The processing part extracts the bright spot of fluorescence generated from the fluorescent dye that labels the target site from the fluorescence image for each of a plurality of cells included in the sample, and generates information used for determining whether the sample is positive or negative based on the bright spots extracted for each of the plurality of cells. 1. A method for analyzing cells in a sample treated with a fluorescent label specifically biding to a target site of cells , comprising:capturing fluorescence images of individual cells in the sample treated with the fluorescent label; anddigitally analyzing at least some of the fluorescence images to identify at least a positive cell having a particular pattern of bright spots based on a color and a number of bright spots, wherein a positive cell is distinguishable from a negative cell according to the pattern of bright spots.2. The method of claim 1 , further comprisinggenerating information of any one selected from a group consisting of: a number of positive cells, a number of negative cells, a ratio of the number of positive cells to the number of negative cells, and a ratio of the number of negative cells to the number of positive cells.3. The method of claim 1 , further comprisinggenerating information of a population of positive cells in the sample.4. The method of ...

Methods And Compositions For Incorporating Nucleotides

The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses. 1. A method for determining an identity of a nucleotide at a position , comprising: a) providing a device capable of measuring color in more than one detector channel , nucleic acid , polymerase , and a mixture of labeled and non-labeled reversible terminators , said labeled reversible terminators comprising a label linked to a nucleotide analogue , said label producing color capable of being detected in two channels; b) incorporating , with said polymerase , a first nucleotide analogue from said mixture into said nucleic acid at a position; c) detecting the label of said incorporated first nucleotide analogue in two channels with said device; and d) determining the identity of the incorporated nucleotide at said position.2. The method of claim 1 , wherein said mixture of labeled and non-labeled reversible terminators is reversibly terminated by an azidomethyl group at the 3′ position.3. The method of claim 1 , wherein said labeled reversible terminators are labeled with at least one type of fluorescent dye and said detecting of step c) comprising detecting color of said at least one type of fluorescent dye in two channels.4. The method of claim 3 , wherein said at least one type of fluorescent dye is visible in the yellow channel and the green channel.5. The method of claim 3 , wherein said at least one type of fluorescent dye is visible ...

METHODS AND DEVICES FOR XEROGEL BASED SENSORS

Chemical sensors today are deployed in massive volumes across multiple industries and yet at the same time they are subject to substantial research and development effort to establish new, faster, lower cost, more accurate, more sensitive chemical sensors. Such sensors and sensor arrays are being exploited across chemistry, biology, clinical biology, environmental science in civilian and military markets. Amongst the many sensor methodologies are xerogel substrates with two moieties, a receptor for molecular recognition of the analyte and a luminophore for signaling the recognition event. In order to fulfill the requirements for low cost there is a requirement for electronic excitation/read circuits that can support architectures with optical source—N sensors—X filters—M detectors, where M≧N and X=N|M. According to embodiments of the invention electronic excitation/read circuits for phase based luminophore sensors are presented that are compatible with single CMOS chip implementation and monolithic integration of the optical excitation/detection elements. 1. A system comprising:a plurality of optical sources disposed in a predetermined relationship with a fluidic channel formed within a silicon substrate, each optical source emitting a first optical signal over a first predetermined wavelength range and capable of analog modulation over a predetermined frequency range;a plurality of sensors each optically coupled to a predetermined subset of the optical sources and disposed in a predetermined relationship with the fluidic channel and its predetermined subset of the optical sources, wherein the plurality of sensors each formed upon a substrate and each incorporating a fluorescent material immobilized to a capture material selected in dependence upon an analyte to be measured and each fluorescent material emits an second optical signal over a second predetermined wavelength range;an optical detection circuit comprising a plurality of optical detectors, each configured ...

METHOD FOR MEASURING SOLID-LIQUID INTERFACIAL ELECTRIC FIELD

An interfacial electric field intensity of a surface is measured by covalently binding a monolayer of a voltage sensitive chromophore to the surface and irradiating it with actinic radiation while it is in contact with a liquid and measuring a first fluorescence emission spectrum. A solution of the voltage sensitive chromophore dissolved in a sample of the liquid is also irradiated with actinic radiation and a second fluorescence emission spectrum is measured. The first and second fluorescence emission spectra are compared to determine the interfacial electric field intensity. 1. A method for measuring an interfacial electric field intensity , comprising:providing a surface having a reactive carbocyclic aromatic linking group covalently attached thereon;providing a voltage sensitive chromophore precursor including a p-substituted dialkylamino aryl group that is conjugatively linked to a terminal N-containing heterocyclic aromatic group;reacting the voltage sensitive chromophore precursor with the reactive carbocyclic aromatic linking group that is covalently attached to the surface to form a monolayer of a voltage sensitive chromophore that is covalently bound to the surface;irradiating the monolayer of the covalently bound voltage sensitive chromophore with actinic radiation while it is in contact with a liquid and measuring a first fluorescence emission spectrum;providing a voltage sensitive chromophore solution of the voltage sensitive chromophore dissolved in a sample of the liquid;irradiating the voltage sensitive chromophore solution with actinic radiation and measuring a second fluorescence emission spectrum; andcomparing the first and second fluorescence emission spectra to determine an interfacial electric field intensity.2. The method of claim 1 , wherein the reactive carbocyclic aromatic linking group includes a benzyl halide group.3. The method of claim 1 , wherein the N-containing heterocyclic aromatic group is a pyridinyl group.4. The method of claim 1 ...

METHOD FOR CHARACTERIZING A LIQUID

A characteristic of a liquid is measured by providing a surface having a monolayer of a voltage sensitive chromophore that is covalently bound to the surface. The liquid is brought into contact with the surface and it is irradiated with actinic radiation to measure a first fluorescence emission spectrum. A solution of the voltage sensitive chromophore dissolved in a sample of the liquid is also irradiated with actinic radiation and a second fluorescence emission spectrum is measured. The first and second fluorescence emission spectra are compared to determine the characteristic of the liquid. 1. A method for determining a characteristic of a liquid , comprising:providing a surface having a reactive carbocyclic aromatic linking group covalently attached thereon;providing a voltage sensitive chromophore precursor including a p-substituted dialkylamino aryl group that is conjugatively linked to a terminal N-containing heterocyclic aromatic group;reacting the voltage sensitive chromophore precursor with the reactive carbocyclic aromatic linking group that is covalently attached to the surface to form a monolayer of a voltage sensitive chromophore that is covalently bound to the surface;bringing the liquid into contact with the monolayer of the covalently bound voltage sensitive chromophore;irradiating the monolayer of the covalently bound voltage sensitive chromophore with actinic radiation while it is in contact with the liquid and measuring a first fluorescence emission spectrum;providing a voltage sensitive chromophore solution of the voltage sensitive chromophore dissolved in a sample of the liquid;irradiating the voltage sensitive chromophore solution with actinic radiation and measuring a second fluorescence emission spectrum; andcomparing the first and second fluorescence emission spectra to determine a characteristic of the liquid.2. The method of claim 1 , wherein the reactive carbocyclic aromatic linking group includes a benzyl halide group.3. The method of ...

Multiplexed fluorescent detection of analytes

In a first aspect, a method includes: providing a sample, the sample including a first nucleotide and a second nucleotide; contacting the sample with a first fluorescent dye and a second fluorescent dye, the first fluorescent dye emitting first emitted light within a first wavelength band responsive to a first excitation illumination light, the second fluorescent dye emitting second emitted light within a second wavelength band responsive to a second excitation illumination light; simultaneously collecting, using one or more image detectors, multiplexed fluorescent light comprising the first emitted light and the second emitted light, the first emitted light being a first color channel corresponding to the first wavelength band and the second emitted light being a second color channel corresponding to the second wavelength band; and identifying the first nucleotide based on the first wavelength band of the first color channel and the second nucleotide based on the second wavelength band of the second color channel.

Spectral Unmixing

Processing of images acquired via fluorescence microscopy by identifying broadband and other undesired signals from the component signals of a scanned image, and processing selected regions of the image that are known to contain signals of interest, thereby extracting or identifying desired signals while subtracting undesired signals. One or more broadband signals are recognized by their unique signature and ubiquitous dispersion through the image. Regions of the scanned image may be tagged as consisting of predominantly broadband signals and are ignored during a spectral unmixing process. The remaining regions of the image, or selected regions of the image known to contain desired signals, may be unmixed, and the plurality of reference spectra subtracted from the components to extract or identify the target signals. The set of target signals may be refined by eliminating known or obvious sources of noise by, for instance, being compared to known or ideal sets of signals from similar materials. 1. A non-transitory computer-readable medium for storing computer-executable instructions that are executed by a processor to perform operations comprising:receiving an image comprising a mixture of signals, the mixture of signals further comprising a measured broadband signal and a target signal;detecting a location of a first region of the image, the first region predominantly comprising the measured broadband signal, the detecting being based on a comparison of the measured broadband signal with a known broadband signal;estimating a reference signal for the image based on the measured broadband signal; andutilizing the reference signal to spectrally unmix the mixture.2. The computer-readable medium of claim 1 , wherein the known broadband signal is retrieved from a calibration image.3. The computer-readable medium of claim 1 , wherein the comparison comprises comparing a signature of the measured broadband signal with a signature of the known broadband signal.4. The ...

In-situ evaluation of curing of ink compositions via fluorescence spectroscopy and related methods

A method for evaluating curing in an ink composition comprises depositing an ink composition on the surface of an object via a direct-to-object inkjet printing system to form a film thereon, the ink composition comprising a photoinitiator capable of initiating a free radical polymerization process in the ink composition upon the absorption of light to cure the deposited film and a fluorophore capable of emitting viscosity-dependent fluorescence upon the absorption of light; exposing, in-situ, the deposited film to light generated by a first source of light under conditions which initiate the free radical polymerization process to cure the deposited film; exposing the cured film to light generated by a second source of light under conditions which induce fluorescence emission by the fluorophore in the cured film; measuring the fluorescence emission; and determining a degree of cure in the cured film from the measured fluorescence emission and predetermined calibration data.

SPECTRAL MICROSCOPE

In one implementation, a spectral microscope may comprise a substrate with a planar lens, the planar lens including a phase profile including an axial focus and an oblique focus, a light source to excite a signal of a particle among a plurality of particles, and a detector to receive light generated from the light source from the axial focus of the planar lens and a spectral color component of the excited signal of the particle from the oblique focus of the planar lens. 1. A spectral microscope , comprising:a substrate with a planar lens patterned on the substrate, the planar lens including a phase profile including an axial focus and an oblique focus;a chip having a channel, wherein the chip is adjacent to the substrate; and light generated from a light source through the chip and the substrate from the axial focus of the planar lens; and', 'a spectral color component of an excited signal of a particle located in the channel through the chip and the substrate from the oblique focus of the planar lens., 'a detector to receive2. The spectral microscope of claim 1 , wherein the planar lens is a diffractive lens.3. The spectral microscope of claim 1 , wherein the detector is positioned above the substrate.4. The spectral microscope of claim 3 , wherein the light source is positioned below the chip to excite the signal of the particle such that the detector positioned above the substrate is to receive the light from the axial focus of the planar lens and the spectral color component of the particle from the oblique focus of the planar lens.5. The spectral microscope of claim 1 , wherein the planar lens is a partially reflective lens to reflect the spectral color component to the detector.6. The spectral microscope of claim 1 , wherein the detector is positioned in the chip and located adjacent to the channel.7. The spectral microscope of claim 6 , wherein the light source is positioned above the chip to excite the signal of the particle such that the detector positioned ...

SOLID-STATE INTEGRATED REAL-TIME OPTICAL MONITORING OF BIOCHEMICAL ASSAYS

The disclosed technology includes a planar device for performing multiple biochemical assays at the same time, or nearly the same time. Each assay may include a biosample including a biochemical, enzyme, DNA, and/or any other biochemical or biological sample. Each assay may include one or more tags including dyes and/or other chemicals/reagents whose optical characteristics change based on chemical characteristics of the biological sample being tested. Each assay may be optically pumped to cause one or more of luminescence, phosphorescence, or fluorescence of the assay that may be detected by one or more optical detectors. For example, an assay may include two tags and a biosample. Each tag may be pumped by different wavelengths of light and may produce different wavelengths of light that is filtered and detected by one or more detectors. The pump wavelengths may be different from one another and different from the produced wavelengths. 1. A planar biochemical device , the device comprising:a plurality of sample holders arranged in a planar configuration forming a one-dimensional or a two-dimensional array, each sample holder configured for an assay comprising one or more biosamples and one or more reagents;a planar heater coupled to the plurality of sample holders, wherein the planar heater is operable to heat the plurality of sample holders;an optical substrate layer coupled to the planar heater, wherein the optical substrate layer distributes light from one or more optical sources to each of the sample holders; andan optical detection layer positioned to receive light from the plurality of sample holders, the optical detection layer including one or more optical detectors in alignment with each sample holder to detect light corresponding to each assay.2. The planar biochemical device of claim 1 , further comprising:one or more optical filters positioned between the optical detection layer and the optical substrate, wherein each optical filter passes light of a ...

Method And Apparatus For Characterising Samples By Measuring Light Scattering and Fluorescence

A method for characterising at least one sample, the method including the steps of a) the lighting of each sample to be analysed by N>1 light rays (LE 1 -LE 3 ) at respective wavelengths of light (λε 1 -λE 3 ); b) the acquisition, for each of the light rays, of at least one fluorescent light intensity and at least one elastic scattering light intensity emitted by each sample; c) determining a vector indicator for each sample based on said fluorescent and elastic scattering light intensities; d) determining at least one parameter characterising each sample, or a method to which the sample was submitted, based on the corresponding vector indicator. Apparatus for implementing such a method is also provided.

Readout & signal transduction (rost) component for poc devices

A readout and signal transduction (ROST) component includes a universal fixture adapted to receive and align one or more optical elements and one or more opto-mechanical elements, wherein the optical elements and one or more opto-mechanical elements are stacked in the universal fixture, one or more optical elements configured for integration into the universal fixture; and one or more opto-mechanical elements configured for integration into the universal fixture.

Particle detector

There is provided a particle detector that can increase a detection sensitivity to fluorescence emitted from biogenic particles. A particle detector for detecting biogenic particles includes a substrate having a principal surface and configured to collect the biogenic particles on the principal surface, a light emitting element configured to irradiate particles collected on the principal surface with excitation light, and a light receiving element configured to receive fluorescence emitted from the particles when the particles are irradiated with the excitation light from the light emitting element. An optical axis of the Fresnel lens and a ray direction of the excitation light intersect with each other. The principal surface is a mirror surface.

Method and System for Imaging and Collection of Data for Diagnostic Purposes

A system for acquiring data regarding a wound in tissue comprises at least one light source configured to directly illuminate a target surface with a homogeneous field of excitation light. An optical sensor is configured to detect signals responsive to illumination of an illuminated portion of a wound and the area around the wound. A thermal sensor is configured to detect thermal information regarding the illuminated portion of the wound and the area around the wound. A processor receives the detected signals and the detected thermal information and outputs data regarding the illuminated portion of the wound and the area around the wound. The output data may include wound size of the illuminated portion of the wound, bacterial load of the illuminated portion of the wound, or at least one temperature associated with the illuminated portion of the wound and the area around the wound. The data output by the processor may be displayed on a display of the system.

SYSTEMS AND METHODS FOR HYPERSPECTRAL IMAGING

A method, and corresponding system, can include identifying a plurality of wavelength spectral band components in hyperspectral image data, the spectral band components corresponding to mutually distinct sources of image contrast. An intensity image corresponding to each respective spectral band component can be calculated, followed by combining the respective intensity images to form an inter-band image based on the respective, mutually distinct sources of image contrast for each spectral band component. Intensity images can be hyperspectral or hyperdiffuse images. Hyperdiffuse imaging can be performed for each spectral band component identified using hyperspectral measurements. Spectral position and spectral width images corresponding to each spectral band component can be calculated and used to determine depth of features inside a surface of the target. Diffuse width images can be calculated from hyperdiffuse image data and used to determine depth. 1. A method comprising:identifying a plurality of wavelength spectral band components in hyperspectral image data, the spectral band components corresponding to mutually distinct sources of image contrast;calculating respective intensity images corresponding to each respective spectral band component;combining the respective intensity images to form inter-band images based on the respective, mutually distinct sources of image contrast for each spectral band component; andproducing a 2D image of enhanced contrast using selection of one or more of the inter-band images that are of greater contrast than one or more others of the inter-band images.2. The method of claim 1 , wherein calculating the respective intensity images includes performing an intra-band pixel-wise analysis of one or more of the spectral band components.3. The method of claim 1 , wherein combining the respective intensity images to form an inter-band image includes performing an inter-band pixel-wise analysis by dividing individual pixel values of one ...

Methods and Compositions for Incorporating Nucleotides

The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

Microparticle measurement device

In a microparticle measurement device, a sample is passed through each channel in a multi-flow channel, and a predetermined linear area is illuminated with light. Measurement light originating from a microparticle in the sample, such as scattered or fluorescent light, is shaped into a parallel beam by an objective lens and passes through a first and second transmission portions. The beams transmitted through these two portions are converged as first and second measurement beams onto the same straight line by a cylindrical lens. The intensity of the interference light formed by these beams is detected with a detector. Meanwhile, the light emitted from the light source and passing through the multi-flow channel without hitting the microparticle falls through the objective lens onto a non-reflection portion and does not travel toward the cylindrical lens. Accordingly, only the interference light formed by the measurement beams is allowed to fall onto the detector.

Methods of mfish using alignment beads

This disclosure relates to methods for image registration in fluorescence assays for the detection and quantitation of analytes in a sample using functionalized alignment beads as fiducial markers to improve image registration.

TWO-COLOR CONFOCAL COLOCALIZATION MICROSCOPY

Disclosed herein are a method and a device for two-color confocal colocalization microscopy of a sample. The sample is labeled by a confocal imaging marker and a stimulated emission depletion (STED) imaging marker. The method comprises generating a first confocal excitation pulse with a first wavelength λand a second confocal excitation pulse with a second wavelength λdifferent from the first wavelength; focusing the first and second confocal excitation pulses onto a confocal focus point; generating a STED excitation pulse with the first wavelength and a STED depletion pulse with the second wavelength; focusing the STED excitation pulse and the STED depletion pulse onto a STED focus point; and detecting light emitted from the sample at an emission wavelength λof the STED imaging marker and at an emission wavelength λof the confocal imaging marker. An n-photon excitation at the first wavelength with n≥ is resonant with an excitation transition of a STED imaging marker; the second wavelength is resonant with a depletion transition of the STED imaging marker; and a two-photon excitation involving a photon having the first wavelength and a photon having the second wavelength is resonant with an excitation transition of a confocal imaging marker.

CONTINUOUS PROCESS FOR PERFORMING MULTIPLE NUCLEIC ACID AMPLIFICATION ASSAYS

A method of determining the presence or amount of a target nucleic acid in each of a plurality of reaction mixtures. In the method, a first plurality of reaction mixtures are provided to a heater and subjected to conditions for performing a first amplification reaction. The presence or amount of a first target nucleic acid in each of the first plurality of reaction mixtures is determined during the first amplification reaction. During the first amplification reaction, a second plurality of reaction mixtures are provided to the heater and subjected to conditions for performing a second amplification reaction. The presence or amount of a second target nucleic acid in each of the second plurality of reaction mixtures is determined during the second amplification reaction. At least a portion of the second plurality of reaction mixtures are removed from the heater during the second amplification reaction. 1. (canceled)2. A method of determining the presence or amount of a target nucleic acid in each of a plurality of reaction mixtures , the method comprising the automated steps of:a) providing a first plurality of reaction mixtures to a heater disposed on a generally horizontal work surface of an analyzer having a window situated above the work surface, wherein each reaction mixture of the first plurality of reaction mixtures is suspected of containing a first target nucleic acid, and wherein each reaction mixture of the first plurality of reaction mixtures contains reagents sufficient to perform a first nucleic acid amplification reaction with the first target nucleic acid;b) in the heater, subjecting each reaction mixture of the first plurality of reaction mixtures to conditions sufficient to perform the first nucleic acid amplification reaction;c) determining the presence or amount of the first target nucleic acid in each reaction mixture of the first plurality of reaction mixtures during the first nucleic acid amplification reaction;d) during steps b) and c), ...

METHODS AND SYSTEMS FOR FLUORESCENCE DETECTION USING INFRARED DYES

Methods and systems for time-domain and wavelength domain resolved multiplexed time-resolved fluorescence of a sample having at least one of a first fluorescent label bound to a first analyte and a second fluorescent label bound to a second analyte. The sample also includes a third fluorescent label bound to a third analyte and a fourth fluorescent label bound to a fourth analyte. The first and second fluorescent labels emit wavelengths having a first and second lifetime. The third fluorescent label comprises an upconverting phosphor (UCP) emitting at a wavelength having a third lifetime, and the fourth fluorescent label comprises an infrared dye emitting at wavelength having a fourth lifetime. The methods and systems measure as a function of time intensities of at least one of more of the first, second, third, and fourth fluorescent labels. 1. A method for multiplexed time-resolved fluorescence (TRF) detection includes: the first fluorescent label emits at a first emission wavelength having a first lifetime,', 'the second fluorescent label emits at a second emission wavelength having a second lifetime,', 'the third fluorescent label comprises an upconverting phosphor (UCP) emitting at a third emission wavelength having a third lifetime, and', 'the fourth fluorescent label comprises an infrared dye emitting infrared light at a fourth emission wavelength having a fourth lifetime;, 'providing one or more samples comprising a first fluorescent label bound to a first analyte, a second fluorescent label bound to a second analyte, a third fluorescent label bound to a third analyte, and a fourth fluorescent label bound to a fourth analyte, wherein'}irradiating the first fluorescent label with a first excitation light at a first excitation wavelength, wherein the first fluorescent label upon irradiation with the first excitation wavelength emits a first detection signal at the first emission wavelength;irradiating the second fluorescent label with a second excitation light ...

Method for detecting fluorescent particles

A method for detecting a fluorescent particle comprises the preparation of a sample solution containing fluorescent particles and a substance that promotes transition of the fluorescent particles from a triplet excited state to a singlet ground state, and calculation of the number of molecules of fluorescent particles present in the prepared sample solution. Calculation of the number of molecules of the fluorescent particles comprises moving the location of a photodetection region of an optical system in the sample solution using the optical system of a confocal microscope or multi-photon microscope, individually detecting fluorescent particles by detecting a light signal from the fluorescent particles present in the photodetection region while moving the location of the photodetection region in the sample solution, and counting the number of fluorescent particles detected during movement of the location of the photodetection region by counting the number of individually detected fluorescent particles.

METHODS FOR NUCLEIC ACID SEQUENCING

Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction. 120.-. (canceled)21. A method of determining the sequence of a template nucleic acid comprising:(i) exposing a complex in a target volume, the complex comprising the template nucleic acid, a primer, and a polymerizing enzyme, to a plurality of types of luminescently labeled nucleotides, wherein each type of luminescently labeled nucleotide has a different luminescent lifetime and/or luminescent intensity;(ii) directing a series of pulses of one or more excitation energies towards a vicinity of the target volume;(iii) detecting a plurality of emitted photons from luminescently labeled nucleotides during sequential incorporation into a nucleic acid comprising the primer; and(iv) identifying the sequence of incorporated nucleotides by determining the lifetime and intensity of the luminescently labeled nucleotides.22. The method of claim 21 , wherein detecting further comprises recording for each detected emitted photon a time duration between detection of the emitted photon and a prior pulse of excitation energy.23. The method of claim 21 , wherein each type of luminescently labeled nucleotide emits photons after being illuminated by a single excitation energy.24. The method of claim 23 , wherein the single excitation energy is in a spectral range of about 470 to about 510 nm claim 23 , about 510 to about 550 nm claim 23 , about 550 to about 580 nm claim 23 , about 580 nm to about 620 nm claim 23 , or about 620 nm to about 670 nm.25. The method of claim 21 , wherein one or more type of luminescently labeled nucleotide emits photons after being illuminated by a first excitation energy claim 21 , and one or more type of luminescently ...

Manufacturing a flowcell with a planar waveguide

Provided in one example is a method of manufacturing a flowcell that includes: forming a core layer, the core layer disposed between a substrate and a nanowell layer, the nanowell layer having nanowells to receive a sample, the core layer having a higher refractive index than the substrate and the nanowell layer; and forming a grating to couple light to the core layer.

AUTOMATIC RESPONSE/LIGHT MEASUREMENT DEVICE AND METHOD THEREFOR

The invention relates to an automatic response/light measurement device and a method therefor, and the purpose is to effectively and quickly perform an optical measurement relating to a reaction with high reliability without increasing a device size. The device is configured to have: a container group in which a plurality of reaction containers are arranged; a measurement mount provided with a plurality of coupling ends that are joinable with apertures of the reaction containers, and have light guide portions that optically connect with the interior of the joined reaction containers; a mount transfer mechanism; a measuring device having a measuring end having at least one light guide portion that is optically connectable to the light guide portions of the coupling ends, that is able to receive light based on an optical state within the reaction containers; an on-mount measuring end transfer mechanism; and a measurement control portion. 1. An automatic response/light measurement device comprising:a container group in which two or more reaction containers are arranged;a plurality of types of specific wavelength measuring devices provided with measuring ends having light guide portions that are optically connectable with the interior of said reaction containers, and that are able to receive light of specific wavelengths or specific wavelength bands based on an optical state within said reaction containers via the measuring ends;a measuring end bundling portion that bundles the plurality of measuring ends in parallel;a measuring end transfer mechanism that makes the bundled measuring ends relatively movable with respect to said container group; anda measurement control portion that, by moving said measuring ends along a movement path that successively passes apertures of said reaction containers, controls said measuring end transfer mechanism such that light guide portions of said measuring ends and the interior of said reaction containers are successively optically ...

Confocal imaging methods and apparatus

The invention provides imaging apparatus and methods useful for obtaining a high resolution image of a sample at rapid scan rates. A rectangular detector array having a horizontal dimension that is longer than the vertical dimension can be used along with imaging optics positioned to direct a rectangular image of a portion of a sample to the rectangular detector array. A scanning device can be configured to scan the sample in a scan-axis dimension, wherein the vertical dimension for the rectangular detector array and the shorter of the two rectangular dimensions for the image are in the scan-axis dimension, and wherein the vertical dimension for the rectangular detector array is short enough to achieve confocality in a single axis.

FLUORESCENCE QUENCHING IMMUNOASSAY

The present invention relates to a method and reagents for determining the presence of or the amount of an analyte in a sample. 2. The method of claim 1 , wherein the analyte is a macromolecule.3. The method of claim 2 , wherein the analyte is a protein.4. The method of claim 3 , wherein (i) the fluorescent tracer is a fluorescent moiety attached to an amino acid chain claim 3 , wherein the length of the amino acid chain is shorter than the amino acid length of the protein claim 3 , and wherein the amino acid sequence of the amino acid chain comprises the amino acid sequence of an epitope on the protein that is responsible for complexing with the binding partner claim 3 , and (ii) the binding partner is optionally conjugated to a quencher.5. The method of claim 4 , wherein the quencher is selected from the group consisting of CY3 claim 4 , CY5 claim 4 , IRDyeQC1 claim 4 , BHQ1 claim 4 , and BHQ10.6. The method of claim 4 , wherein the binding partner is an antibody.7. The method of claim 4 , wherein the protein is selected from the group consisting of canine CysB protein and canine NT-proBNP.8. The method of claim 7 , wherein the protein is canine CysB protein.9. The method of claim 8 , wherein the binding partner is an anti-cystatin-B antibody and the fluorescent tracer is fluorescein attached to an amino acid sequence selected from the group consisting of SEQ ID NO: 2 claim 8 , SEQ ID NO: 3 claim 8 , SEQ ID NO: 4 claim 8 , SEQ ID NO: 5 claim 8 , SEQ ID NO: 6 claim 8 , and SEQ ID NO: 7.10. The method of claim 9 , wherein the amino acid sequence is attached to the 5-position of the fluorescein.11. The method of claim 10 , wherein the amino acid sequence is attached to the 5-position of the fluorescein with a —NH—C(O)— linker.12. The method of claim 10 , wherein the anti-cystatin-B antibody is conjugated to a quencher.13. The method of claim 9 , wherein the amino acid sequence is attached to the 4′-position of the fluorescein.14. The method of claim 13 , wherein the ...

Detector Device for Detection of a Spectral Portion for a Microscope

A detection device for a microscope comprises a dispersive element in the beam path of light and a selection element. The selection element separates a beam path of a spectral portion of the light from the beam path of the light. The detector device furthermore comprises a focusing optical unit configured to focus the beam path of the spectral portion of the light onto a sensor. By way of example, the microscope may be a confocal microscope. 1. A detector device for a microscope , wherein the detector device comprises:a dispersive element in the beam path of the light;a selection element, wherein the selection element is arranged downstream of the dispersive clement in a beam path of the light in such a way that it separates a beam path of a spectral portion of the light from the beam path of the light;a focusing optical unit arranged downstream of the selection element in the beam path of the spectral portion of the light, wherein the focusing optical unit is configured to focus the beam path of the spectral portion of the light onto a sensor; andthe sensor arranged downstream of the focusing optical unit in the beam path of the spectral portion of the light.2. The detector device as claimed in claim 1 ,wherein the focusing optical unit comprises a transverse chromatic aberration correction element arranged in the beam path of the spectral portion of the light,wherein the selection element comprises at least one wedge-shaped prism,wherein the transverse chromatic aberration correction element comprises at least one wedge-shaped prism, andwherein the selection element and the transverse chromatic aberration correction element are arranged relative to one another in such a way that a transverse chromatic aberration of the at least one wedge-shaped prism of the selection element counteracts a further transverse chromatic aberration of the at least one wedge-shaped prism of the transverse chromatic aberration correction element.3. The detector device as claimed in ...

FINE PARTICLE ANALYZING APPARATUS, FINE PARTICLE ANALYZING METHOD, PROGRAM, AND FINE PARTICLE ANALYZING SYSTEM

Provided are a fine particle analyzing apparatus, a fine particle analyzing method, a program, and a fine particle analyzing system, which are capable of easily separating a plurality of types of spectral data on fluorescence emitted from a fine particle. 1. A fine particle analyzing apparatus including a data extracting unit that selectively extracts spectral data , which contain predetermined information , from spectral data on fluorescence emitted from a fine particle.2. The fine particle analyzing apparatus according to claim 1 , wherein the data extracting unit selectively extracts spectral data indicating the maximum intensity in a wavelength area set beforehand from one or a plurality of types of spectral data indicating intensity of fluorescence emitted from the fine particle for each of a plurality of wavelengths.3. The fine particle analyzing apparatus according to claim 2 , wherein the data extracting unit removes spectral data indicating the maximum intensity lower than a predetermined intensity from the one or the plurality of types of spectral data.4. The fine particle analyzing apparatus according to claim 2 , comprisinga display adjusting unit that allows display of the one or the plurality of types of spectral data, whereinwhen the spectral data has been extracted by the data extracting unit, the display adjusting unit allows selective display of the extracted spectral data.5. The fine particle analyzing apparatus according to claim 4 , comprisinga data obtaining unit that obtains the one or the plurality of types of spectral data, whereinthe display adjusting unit allows overlapped display of the spectral data obtained by the data obtaining unit.6. The fine particle analyzing apparatus according to claim 5 , wherein the display adjusting unit varies display colors of the spectral data in accordance with frequency of overlap between the spectral data.7. The fine particle analyzing apparatus according to claim 5 , wherein the display adjusting unit ...

Unknown Sample Determining Method, Unknown Sample Determining Instrument, and Unknown Sample Determining Program

In a standard process for determining an unknown sample, fluorescent substances are determined from respective fluorescence characteristics and model coefficients are calculated from spectrum ranges of the fluorescence characteristics of the determined fluorescent substances. An unknown sample is measured after reading of the model coefficients, whereby a target value of the unknown sample is obtained.

SYSTEM AND METHOD WITH REFLECTIVE FIDUCIALS

Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducials may have configurations that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducials may include features and materials that are provided on or in the support of the array and that return at least a portion of incident light by reflection. The fiducials may form gratings or other encoding configurations that assist in image processing, alignment, or other aspects of processing of the array. 1. An array , comprising:a support having locations that, in operation, receive biological samples differing from one another to respond differently in successive cycles of fluorescent imaging; anda fiducial feature that is formed in or on the support and that is optically reflective to, during imaging, return at least a portion of incident radiation for locating, adjusting the location of, or registering the support or the locations.2. The array of claim 1 , wherein the fiducial feature is disposed over a surface of the support.3. The array of claim 1 , wherein the fiducial feature is formed into the support.4. The array of claim 1 , wherein the fiducial feature lies outside of an area in which the locations are provided.5. The array of claim 1 , wherein the fiducial feature comprises a Bragg grating.6. The array of claim 1 , wherein the support comprises a glass material claim 1 , a semiconductor material claim 1 , or combinations thereof claim 1 , and wherein the fiducial feature comprises a metallic material.7. The array of claim 1 , wherein in performing the successive cycles of fluorescent imaging only some of the biological samples appear in successive images to the exclusion of other of the biological samples claim 1 , but in which the fiducial feature appears in all images.8. The array of claim 1 , wherein the ...

Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection

Systems and methods for performing simultaneous nucleic acid amplification and detection. The systems and methods comprise methods for managing a plurality of protocols in conjunction with directing a sensor array across each of a plurality of reaction chambers. In certain embodiments, the protocols comprise thermocycling profiles and the methods may introduce offsets and duration extensions into the thermocycling profiles to achieve more efficient detection behavior.

Method for detecting a target particle

This method for detecting a target particle has: (a) a step for preparing a sample solution containing target particles and one type or two or more types of a luminescent probe that binds to the target particles, and allowing two or more molecules of the luminescent probe to bind per one molecule of the target particles in the sample solution, and (b) a step for calculating the number of molecules of target particles bound to the luminescent probe present in the sample solution prepared in step (a) by a scanning molecule counting method by using as an indicator thereof the strength of light signals of the individually detected particles, and the luminescent probe is one type or two or more types of a luminescent probe to which the same type of luminescent substance is bound.

METHODS FOR QUANTIFYING LIGAND EFFICACY IN G-PROTEIN COUPLED RECEPTORS USING SINGLE-MOLECULE FLUORESCENCE ENERGY TRANSFER

Disclosed herein are methods for providing a molecular efficacy of a ligand, especially when utilizing single-molecule fluorescence resonance energy transfer (“smFRET”) imaging, as well as compounds useful in such methods. 1. A method for providing a molecular efficacy of a ligand , the method comprising normalizing an effective rate of generating G(GTP) from G(GDP) provided by the ligand to an effective rate of adrenaline in generating G(GTP) from G(GDP) to provide a normalized value η; and{'sub': 50', '50', '2, 'normalizing an apparent ECfor GDP binding of the ligand to an apparent ECfor GDP binding of adrenaline to provide a normalized value η;'}{'sub': 1', '2, 'wherein a value greater than 1.0 for the multiple of ηand ηindicates the ligand is more efficacious than adrenaline.'}2. The method of claim 1 , wherein the method further comprises{'sub': S', '2', 'S, 'single-molecule fluorescence resonance energy transfer (“smFRET”) imaging of a complex of Gwith Cy3B*/Cy7*-labelled βΔ6-N148C/L266C in the presence of a saturating concentration of the ligand, a concentration of G, and a concentration of apyrase.'}3. The method of claim 2 , wherein the method further comprises{'sub': 2', 'S, 'smFRET imaging of Cy3B*/Cy7*-labelled βΔ6-N148C/L266C in the presence of a saturating concentration of the ligand, a concentration of GDP, and two or more different concentrations of G;'}{'sub': S', 'S, 'wherein at least two of the two or more different concentrations of Gare each below a saturating concentration of G.'}4. (canceled)5. A method for providing a molecular efficacy of a ligand claim 2 , the method comprising detecting an effective rate of generating G(GTP) from G(GDP) provided by the ligand (“ligand effective rate”); and{'sub': 50', '50, 'detecting an apparent ECfor GDP binding of the ligand (“ligand EC”);'} [{'sub': 1', '2, 'a value greater than 1.0 for the product of ηand ηindicates the ligand is more efficacious than adrenaline;'}, {'sub': 1', 'S', 'S, 'ηis the ligand ...

MEMBRANE-COUPLED CONTINUOUS SENSING SYSTEMS

A device () for sensing a first analyte in a biofluid is provided. The device includes a microfluidic component () configured to transport a biofluid sample. The device further includes a sensing solution () containing one or more probes, wherein the one or more probes are configured to interact with a first analyte in the biofluid. The device further includes a sensor membrane () separating the sensing solution () from the microfluidic component (), the sensor membrane () configured to allow transport of the first analyte from the microfluidic component to the sensing solution and prevent transport of the one or more probes out of the sensing solution. The device further includes a sensor configured to sense a reaction between the one or more probes and the first analyte in the sensing solution (). 1. A device comprising:a microfluidic component configured to transport a biofluid sample;a sensing solution containing one or more probes, wherein the one or more probes are configured to interact with a first analyte in the biofluid;a sensor membrane separating the sensing solution from the microfluidic component, the sensor membrane configured to allow transport of the first analyte from the microfluidic component to the sensing solution and prevent transport of the one or more probes out of the sensing solution; anda sensor configured to sense a reaction between the one or more probes and the first analyte in the sensing solution.2. The device of claim 1 , further comprising:a buffer including one or more buffering solutes; anda buffer membrane separating the buffer from the microfluidic component, the buffer membrane configured to allow transport of the one or more buffering solutes into the microfluidic component and to prevent the first analyte from entering the buffer.3. The device of claim 1 , wherein the microfluidic component comprises at least one of a wicking material or a microfluidic channel.4. The device of claim 3 , wherein the microfluidic component ...

METHOD AND APPARATUS FOR PATHOGEN TESTING

A method of testing for pathogens can include applying a pathogen indicating substance to an object, the pathogen indicating substance having one characteristic when not in contact with a pathogen and another characteristic when in contact with a pathogen, and generating a signal indicative of the level of pathogen contamination on the object by quantifying the presence of the pathogen indicating substance with the pathogen indicating characteristic on the object. An apparatus for testing for pathogens can include a dispenser for dispensing a pathogen indicating substance, the pathogen indicating substance having one characteristic which is altered to another characteristic on contact with a pathogen, a main sensor for detecting a level of pathogen contamination by quantifying the pathogen indicating substance having the pathogen indicating characteristic, and a control unit for generating a signal indicative of the level of pathogen contamination detected by the sensor. 1. A method of testing for at least one pathogen , the method comprising:applying a pathogen indicating substance to an object, the pathogen indicating substance having a first characteristic when not in contact with the pathogen and a second characteristic when in contact with the pathogen; andgenerating a signal indicative of the level of pathogen contamination on the object by quantifying the presence of the pathogen indicating substance with the second characteristic on the object.2. The method of claim 1 , wherein the object comprises one or more human hands.3. The method of claim 1 , wherein the first and second characteristics comprise optical characteristics.4. The method of claim 3 , wherein the first characteristic comprises a first type of fluorescence claim 3 , and the second characteristic comprises a second type of fluorescence.5. The method of claim 3 , wherein the quantifying comprises using an optical sensor.6. The method of claim 1 , wherein the generating comprises generating a ...

Protein structure analysis based on red-edge excitation shift (rees) spectroscopy

A protein structure analysis method includes receiving a fluorescence emission spectrum generated by a protein sample at a first excitation wavelength, evaluating, from the fluorescence emission spectrum, an indication characteristic of a fluorescence response of the protein sample at the first excitation wavelength, and repeating the receiving and evaluating steps in relation to a plurality of fluorescence emission spectra generated by the protein sample at a different excitation wavelength to the first excitation wavelength. In addition, the method includes generating a non-linear relationship between excitation wavelength and indication characteristic of fluorescence response of the protein sample that includes an indication of at least one characteristic of the conformational state of the protein sample. The method can be used with any protein which includes one or more naturally occurring fluorescent amino acids (intrinsic fluorophores), and particularly those with Trp residues (most), or proteins having appropriately selected extrinsic fluorophores.

OPTICAL MICROSCOPY WITH PHOTOTRANSFORMABLE OPTICAL LABELS

Imaging a sample that includes phototransformable optical labels (“PTOLs”) with an optical system having a diffraction-limited resolution volume (DLRV), includes providing activation radiation to the PTOLs to activate a statistical subset of the PTOLs. A density of the PTOLs of the activated subset is less than an inverse of the DLRV. Excitation radiation is provided to the activated subset to excite activated PTOLs. Radiation emitted from the activated and excited PTOLs located at different focal planes of the optical system within the sample is detected with the optical system. The preceding steps are repeated one or more times, each time activating a different statistical subset of the plurality of PTOLs. Three-dimensional locations within the sample are determined, with a sub-diffraction-limited accuracy, of the activated and excited PTOLs based on the radiation emitted from the activated and excited PTOLs that is detected from the different focal planes of the optical system. 1providing activation radiation to a sample, wherein the sample includes phototransformable optical labels (“PTOLs”) distributed in the sample such that separation distances between PTOLs are less than a diffraction limited resolution of a detection objective of imaging optics used to image radiation from the PTOLs, to activate a subset of the PTOLs in the sample;providing excitation radiation to the sample to excite at least some of the activated subset of PTOLs;detecting, with the imaging optics, radiation emitted from the activated and excited PTOLs within the subset of PTOLs;controlling the activation radiation such that adjacent activated PTOLs in a focal plane of the detection objective of the imaging optics are separated by distances that are, on average, larger than the diffraction limited resolution of the detection objective;determining locations of the PTOLs based on the detected radiation; andcreating a superresolution image of the sample based on the determined locations of ...

System for Conducting the Identification of Bacteria in Biological Samples

The present invention relates to a system for conducting the identification and quantification of micro-organisms, e.g., bacteria in biological samples. More particularly, the invention relates to a system comprising a disposable cartridge and an optical cup or cuvette having a tapered surface; an optics system including an optical reader and a thermal controller; an optical analyzer; a cooling system; and an improved spectrometer. The system may utilize the disposable cartridge in the sample processor and the optical cup or cuvette in the optical analyzer. 128-. (canceled)29. An optical reader for analyzing bacteria in a biological specimen , comprising:an optics cup containing the biological specimen;an illumination arrangement including a xenon light source and a system of turning mirrors, filters and a filter wheel supported in a plurality of carriages for producing an illumination beam;said plurality of carriages being arranged at an angle so as to decrease the distance between the light source and the optics cup and to increase the signal-to-noise ratio of the illumination beam;an anchor shoe for supporting the optics cup and having a slit for producing a collimated beam from the illumination beam and directing the collimated beam into the optics cup containing the biological sample to produce fluorescent emissions; andan optical collection device for receiving the fluorescent emissions from the biological specimen and the optics cup and directing the fluorescent emissions to a detection device for the analysis of bacteria in the biological specimen.30. The optical reader of claim 29 , wherein the filter wheel includes a plurality of narrowband filters and wherein the filter wheel is selectively employed to change the narrowband filters so that the radiation from the light source is restricted to a narrow band of ultraviolet light ranging from 260 nm to about 300 nm.31. The optical reader of claim 29 , wherein the optics cup includes a rectangular-shaped ...

Methods and Compositions for Incorporating Nucleotides

The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses. 112-. (canceled)13. A method for determining an identity of a nucleotide at a position , comprising: a) providing nucleic acid , polymerase , and a mixture of labeled and non-labeled reversible terminators , said labeled reversible terminators comprising a label linked to a nucleotide analogue , said label producing color , wherein the label is Rhodamine 6G and is attached to the nucleotide analogue of dUTP though a cleavable linker and wherein a removable chemical moiety caps the 3′-O group , wherein said labeled reversible terminator is in a mixture in solution with an unlabeled reversible terminator comprising a nucleotide analogue of dUTP , wherein the concentration of the unlabeled nucleotide analogue of dUTP is present in said solution at a higher concentration than the labeled nucleotide analogue of dUTP; b) incorporating , with said polymerase , a first nucleotide analogue from said mixture into said nucleic acid at a position; and c) detecting the label of said incorporated first nucleotide analogue.14. The method of claim 13 , wherein said detecting is done with a device.15. The method of claim 14 , wherein said device measures color in more than one detector channel.16. The method of claim 15 , wherein said color detected in two channels.17. The method of claim 13 , wherein said cleavable linker comprises a disulfide bond.18. ...

METHOD AND SYSTEM FOR ITEM AUTHENTICATION AND CUSTOMIZATION

A method of illumating an item is disclosed. The method includes applying adhesive to the item, interspersing a taggant in the adhesive, illuminating the item with an excitation signal, sensing luminescence emitted by the taggant in response to illumination by the excitation signal, and determining the authenticity of the item based on the sensed emitted luminescence. The item can include any item benefited by authentication, and can include a postage stamp. A method of customizing an item is disclosed. This can include the steps of preparing a substrate, applying a security feature to the substrate, printing non-customized information on the substrate, receiving image information, and printing the image information on the substrate. 1. A label comprising:a substrate having a plurality of cells defined thereon;a taggant configured to emit luminescence upon excitation by an excitation signal;wherein the taggant is applied to form a first pattern in one or more of the plurality of cells; andwherein the one or more of the plurality of cells to which the taggant is applied forms a second pattern.2. The label of claim 1 , wherein the first pattern is a predetermined formed within a predetermined number of the one or more of the plurality of cells.3. The label of claim 2 , wherein the predetermined number of cells in which the first pattern is formed forms the second pattern.4. The label of claim 3 , wherein the first pattern and the second pattern are similar claim 3 , and are detectable and recognizable by a detector.5. The label of claim 3 , wherein the first pattern and the second pattern are different claim 3 , and are detectable and recognizable by a detector.6. The label of claim 1 , further comprising an adhesive in contact with the substrate.7. The label of claim 6 , further comprising an object removably attached to the substrate via the adhesive.8. The label of claim 1 , wherein the plurality of cells are divided by a plurality of cell walls.9. The label of ...

Method of measuring plant nutrient transport using near-infrared imaging

An imaging system illuminates a portion of a plant with a light source comprising a near-infrared (NIR) light source to cause fluorescent emission by a fluorescent imaging agent absorbed by the plant and distributed within the plant via transpiration. The imaging system receives the fluorescent emission from the illuminated portion of the plant. The imaging system generates a fluorescent image of the portion of the plant from the received fluorescent emission. The imaging system displays, on a display device of the imaging system, the fluorescent image. Based on the fluorescent image, a determination that the plant has a disease or injury can be made.

METHOD OF DETECTING NUCLEIC ACIDS, METHOD OF OPTICALLY OBSERVING SAMPLE AND FLUORESCENT SUBSTANCE

[Object] A method of detecting nucleic acids easily without requiring complicated operations such as mixing of liquids and cleaning within micro-scale flow channels. 1. A microchip , comprising:a detection region that includes copper; anda sample inlet configured to introduce nucleic acids into the detection region.2. The microchip according to claim 1 , wherein the copper is solid copper claim 1 , a solid that contains copper claim 1 , or an alloy that contains copper.3. The microchip according to claim 1 , further comprising at least a flow channel connected to the detection region.4. The microchip according to claim 1 , wherein the detection region further includes a salt.5. The microchip according to claim 4 , wherein the salt is selected from a group consisting of NaCl claim 4 , KCl or MgCl.6. The microchip according to claim 4 , wherein the salt is disposed as a solution that has a concentration of at least 10 mM.7. The microchip according to claim 1 , wherein a form of the copper is selected from a group consisting of a powder claim 1 , fine particles claim 1 , a rod claim 1 , a wire claim 1 , a plate or a foil.8. The microchip according to claim 1 , wherein an amount of the copper in a powder form to be contacted with a sample is greater than or equal to 37.5 mg per ml of the sample.9. The microchip according to claim 1 , further comprising a pre-treatment region configured to pre-treat a sample.10. A microchip claim 1 , comprising:a detection region that includes a solution that contains copper; anda sample inlet configured to introduce nucleic acids into the detection region. The present application is a continuation application of U.S. patent application Ser. No. 13/882,909, filed May 1, 2013, which is a National Stage of PCT/JP2011/006163, filed Nov. 4, 2011, and claims the priority from prior Japanese Priority Patent Application JP 2010-252517 filed in the Japan Patent Office on Nov. 11, 2010 and JP 2011-201542 filed in the Japan Patent Office on Sep. ...

MICROPARTICLE ANALYZING APPARATUS AND DATA DISPLAYING METHOD

Disclosed herein is a microparticle analyzing apparatus including a detecting portion configured to simultaneously detect a fluorescence generated from a microparticle in plural wavelength regions and a displaying portion configured to display thereon detection results in the plural wavelength regions in a form of a spectrum. 1. A signal processing method , comprising:displaying detected data which indicates a fluorescence generated from a microparticle in plural wavelength regions, wherein the detected data is in a form of a spectrum for each wavelength;detecting a gated area of the detected data; anddisplaying data of the gated area in a form of a spectrum.2. The method according to claim 1 , wherein a height claim 1 , a width or an area of a voltage pulse obtained by converting thereinto a detected light is used as the detected data.3. The method according to claim 1 , wherein the spectrum is displayed in a form of a density plot claim 1 , a dot plot or a contour line plot.4. The method according to claim 1 , wherein only data in a specific region selected from the spectrum is extracted and displayed.5. The method according to claim 4 , wherein the data extracted is two-dimensionally plotted.6. The method according to claim 1 , wherein plural microparticles are continuously detected claim 1 , and results obtained by arithmetically operating the detected data are displayed.7. The method according to claim 1 , wherein the detected data is temporarily preserved claim 1 , and the data thus preserved is read out and displayed.8. The method according to claim 1 , wherein the detected data in each one of the plural wavelength regions has a plurality of colors claim 1 , and a respective color at each fluorescence intensity in each wavelength region indicates an accumulated number of microparticles at said fluorescence intensity in said wavelength region.9. A non-transitory computer readable storage medium storing one or more computer programs adapted to cause a processor ...

INTEGRATED DEVICE WITH EXTERNAL LIGHT SOURCE FOR PROBING DETECTING AND ANALYZING MOLECULES

System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes at least one waveguide configured to propagate excitation energy to the sample wells from a region of the integrated device configured to couple with an excitation energy source. A pixel may also include at least one element for directing the emission energy towards a sensor within the pixel. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device. One of multiple markers distinguishable by temporal parameters of the emission energy may label the sample and configuration of the sensor within a pixel may allow for detection of a temporal parameter associated with the marker labeling the sample. 1. (canceled)2. An integrated device , comprising: a sample well configured to receive excitation energy from an excitation source external to the integrated device; and', 'at least one sensor positioned to receive luminescence from a sample positioned in the sample well and generate a signal that provides identification information of the sample based on the received luminescence., 'a plurality of pixels, wherein a pixel of the plurality of pixels comprises3. The integrated device of claim 2 , further comprising at least one waveguide configured to deliver the excitation energy to a vicinity of the sample well.4. The integrated device of claim 3 , further comprising an excitation source coupling region.5. The integrated device of claim 4 , wherein the excitation source coupling region comprises:a coupling component including a grating coupler, wherein the grating coupler is configured to receive the excitation ...

SINGLE LIGHT SOURCE, TWO-OPTICAL CHANNEL SEQUENCING

Disclosed is a system for determining the nucleotide sequence of polynucleotides. The system can comprise a light source, such as a laser or a LED, configured to generate light at a predetermined wavelength. A detector of the system can detect fluorescent emissions at a first wavelength and a second wavelength. A processor of the system identify the nucleotide as a first type if no fluorescent emission is detected by the at least one detector; identify the nucleotide as a second type if a fluorescent emission at the first wavelength of light is detected by the at least one detector; identify the nucleotide as a third type if a fluorescent emission at the second wavelength of light is detected by the at least one detector; and identify the nucleotide as a fourth type if fluorescent emissions at the first wavelength and the second wavelength of light are detected by the at least one detector. 1. A system for determining the nucleotide sequence of polynucleotides , comprising:a single light source configured to stimulate emission of fluorescent light;at least one detector configured to detect fluorescent emissions off a fluorophore attached to a nucleotide, the at least one detector being configured to detect the fluorescent emissions at a first wavelength and a second wavelength; generating light from the light source onto a nucleotide;', 'identifying the nucleotide as a first type when no fluorescent emission is detected by the at least one detector;', 'identifying the nucleotide as a second type when a fluorescent emission at the first wavelength of light is detected by the at least one detector;', 'identifying the nucleotide as a third type when a fluorescent emission at the second wavelength of light is detected by the at least one detector; and', 'identifying the nucleotide as a fourth type when fluorescent emissions from the nucleotide at the first wavelength and the second wavelength of light are detected by the at least one detector., 'a processor configured ...

Multi excitation-multi emission fluorometer for multiparameter water quality monitoring

A fluorometer is provided for monitoring the quality of water, featuring an array of excitation sources, an array of multiple emission detectors and a signal processor. In the array of excitation sources, each excitation source provides respective excitation source optical signaling at a respective illuminating wavelength. The array of multiple emission detectors detects multiple emission wavelengths emitted from water containing information about multiple coexisting fluorescent species present in the water that emit optical radiation at at least two different wavelengths when illuminated by the respective illuminating wavelength provided from the array of excitation sources, and provide multiple emission detector signaling containing information about the multiple coexisting fluorescent species. The signal processor receives the multiple emission detector signaling, and determines corresponding signaling containing information about an identification of the multiple coexisting fluorescent species present in the water using a near-simultaneous identification technique, based upon the multiple emission detector signaling received.

REACTION PROCESSING APPARATUS

A reaction processing apparatus includes: a reaction processing vessel; a first fluorescence detection device that irradiates a sample with first excitation light and detects first fluorescence produced from the sample; and a second fluorescence detection device that irradiates a sample with second excitation light and detects second fluorescence produced from the sample. The wavelength range of the first fluorescence and the wavelength range of the second excitation light overlap at least partially. The first excitation light and the second excitation light flash at a predetermined duty ratio d. The phase difference between the flashing of the first excitation light and the flashing of the second excitation light is set within a range of 2π(pm−Δpm) (rad) to 2π(pm+Δpm) (rad) or within a range of 2π[(1−pm)−Δpm] (rad) to 2π[(1−pm)+Δpm] (rad), where pm=d−d2 and Δpm=0.01*pm. 1. A reaction processing apparatus comprising:a reaction processing vessel in which a channel where a sample moves is formed;a first fluorescence detection device that irradiates a sample inside a first fluorescence detection region set in the channel with first excitation light and also detects first fluorescence produced from the sample by the irradiation with the first excitation light; anda second fluorescence detection device that irradiates a sample inside a second fluorescence detection region set in the channel with second excitation light and also detects second fluorescence produced from the sample by the irradiation with the second excitation light, whereinthe wavelength range of the first fluorescence and the wavelength range of the second excitation light overlap with each other at least partially,the first excitation light and the second excitation light flash at a predetermined duty ratio, and{'sub': m', 'm', 'm', 'm', 'm', 'm', 'm', 'm', 'm', 'm', 'm, 'sup': '2', 'given that the duty ratio of the flashing of the first excitation light and the flashing of the second excitation light ...

Flow Cell Modules and Liquid Sample Analyzers and Methods Including Same

A liquid sample analyzer includes a liquid sample source, a flow cell, an optical device and a plurality of optical fibers. The flow cell is configured to receive a flow of a liquid sample from the liquid sample source. The plurality of optical fibers optically connect the flow cell to the optical device to transmit light between the flow cell and the optical device.

Method and system for cell detection and analysis

The present invention is a method and a system of cell detection and analysis. The present invention may incorporate at least an optical source, a fluidic chip and a detection module. Cells may be caused to flow within the fluidic chip and specifically past a detection window section accessible by the optical source. The flowing cells may be identified and/or analyzed. The detection module may specifically count the cells of interest as they flow past the detection window section of the chip. The detection module may further be operable to generate or otherwise capture images of the cells as they flow past the window and to use these images collectively for the purpose of analyzing the cells. The present invention may be portable and operable in remote locations.

MICROFLUIDIC SYSTEM FOR AMPLIFYING AND DETECTING POLYNUCLEOTIDES IN PARALLEL

The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.

INTEGRATED DEVICE WITH EXTERNAL LIGHT SOURCE FOR PROBING DETECTING AND ANALYZING MOLECULES

System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes at least one waveguide configured to propagate excitation energy to the sample wells from a region of the integrated device configured to couple with an excitation energy source. A pixel may also include at least one element for directing the emission energy towards a sensor within the pixel. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device. One of multiple markers distinguishable by temporal parameters of the emission energy may label the sample and configuration of the sensor within a pixel may allow for detection of a temporal parameter associated with the marker labeling the sample. 1. An integrated device , comprising:a sample well arranged on a substrate and configured to receive a sample;a waveguide configured to propagate a plurality of pulses of optical excitation energy to the sample well; andat least one sensor, positioned to receive light from the sample well, the at least one sensor configured to obtain a lifetime measurement and a spectral measurement for received photons of luminescence emitted by the sample.2. The integrated device of claim 1 , wherein the at least one sensor comprises a first photosensitive region and a second photosensitive region claim 1 , the first and second photosensitive regions respectively configured to detect a first wavelength range and a second wavelength range.3. The integrated device of claim 2 , wherein the at least one sensor is configured to aggregate claim 2 , into a first plurality of time bins claim 2 , charge carriers generated by photons ...

MULTIPLEXED FLOW ASSAY BASED ON ABSORPTION-ENCODED MICRO BEADS

Analysis of a system and/or sample involves the use of absorption-encoded micro beads. Each type of micro bead is encoded with amounts of the k dyes in a proportional relationship that is different from proportional relationships of the k dyes of others of the n types of absorption-encoded micro beads. A system and/or a sample can be analyzed using information obtained from detecting the one or more types of absorption-encoded micro beads. 1. An analyzer comprising:k light sources configured to selectively stimulate light emission from k dyes, each k dye having an absorption spectrum that is distinguishable from absorption spectra of others of the k dyes, the selective stimulation the k dyes including stimulating each k dye without substantially stimulating others of the k dyes, the k dyes encoding n types of absorption-encoded micro beads, each type of absorption-encoded micro bead encoded with amounts of the k dyes in a proportional relationship that is different from proportional relationships of the k dyes of others of the n types of absorption-encoded micro beads;one or more photosensitive detectors configured to sense the light emission from the k dyes and to generate an output signal; anda processor configured analyze the output signal to detect the n types of absorption-encoded micro beads based on the light emission from the k dyes and to analyze one or more properties of a system using information obtained from one or more types of absorption-encoded micro beads present in a sample.2. The system of claim 1 , wherein excitation light emitted by the k light sources is modulated claim 1 , respectively claim 1 , at k frequencies claim 1 , v claim 1 , v claim 1 , . . . v;the processor is configured to calculate a frequency spectrum of the output signal of the photosensitive detector and to detect the n types of the absorption-encoded micro beads using the frequency spectrum of the output signal.3. A method comprising:using n types of absorption-encoded micro ...

APPARATUS AND METHOD FOR ANALYZING A BODILY SAMPLE

Apparatus and methods are described including successively acquiring a plurality of microscopic images of a portion of a blood sample, and tracking motion of pixels within the successively acquired microscopic images. Trypomastigote parasite candidates within the blood sample are identified, by identifying pixel motion that is typical of trypomastigote parasites. It is determined that the blood sample is infected with trypomastigote parasites, at least partially in response thereto. An output is generated indicating that that the blood sample is infected with trypomastigote parasites. Other applications are also described. 1. A method comprising:successively acquiring a plurality of microscopic images of a portion of a blood sample;tracking motion of pixels within the successively acquired microscopic images;identifying trypomastigote parasite candidates within the blood sample, by identifying pixel motion that is typical of trypomastigote parasites;determining that the blood sample is infected with trypomastigote parasites, at least partially in response thereto; andgenerating an output indicating that that the blood sample is infected with trypomastigote parasites.2. The method according to claim 1 , wherein identifying trypomastigote parasite candidates within the blood sample further comprises identifying entities having a worm-like shape.3. The method according to claim 1 , wherein identifying pixel motion that is typical of trypomastigote parasites comprises identifying entities that undergo a twirling motion.4. The method according to claim 1 , wherein identifying pixel motion that is typical of trypomastigote parasites comprises identifying entities that undergo a spinning motion.5. The method according to claim 1 , wherein successively acquiring the plurality of microscopic images of the portion of the blood sample comprises successively acquiring a plurality of fluorescent microscopic images of a portion of a blood sample claim 1 , and wherein identifying ...

Time-resolved laser-induced fluorescence spectroscopy systems and uses thereof

The invention provides systems for characterizing a biological sample by analyzing emission of fluorescent light from the biological sample upon excitation and methods for using the same. The system includes a laser source, collection fibers, a demultiplexer and an optical delay device. All references cited herein are incorporated by reference in their entirety as though fully set forth. Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

Observation apparatus and observation method

An observation apparatus and an observation method are provided. The observation apparatus includes a light source that emits illumination light used to observe a specimen to which a fluorescent substance that specifically binds to or is expressed in a stimulus target has been supplied, an illumination optical system that radiates the illumination light emitted from the light source 11 onto the specimen, a deflecting device that changes an area of the specimen to be irradiated with the illumination light, a wavelength selecting section that selects the wavelength of illumination light to be radiated onto the specimen, an observation optical system that collects light from the specimen, a detector that detects the light collected by the observation optical system, an image processing section that generates an image from the light detected by the detector, and a control section that controls these components.

SUB-DIFFRACTION LIMIT IMAGE RESOLUTION IN THREE DIMENSIONS

The present invention generally relates to sub-diffraction limit image resolution and other imaging techniques, including imaging in three dimensions. In one aspect, the invention is directed to determining and/or imaging light from two or more entities separated by a distance less than the diffraction limit of the incident light. For example, the entities may be separated by a distance of less than about 1000 nm, or less than about 300 nm for visible light. In some cases, the position of the entities can be determined in all three spatial dimensions (i.e., in the x, y, and z directions), and in certain cases, the positions in all three dimensions can be determined to an accuracy of less than about 1000 nm. In one set of embodiments, the entities may be selectively activatable, i.e., one entity can be activated to produce light, without activating other entities. A first entity may be activated and determined (e.g., by determining light emitted by the entity), then a second entity may be activated and determined. The emitted light may be used to determine the x and y positions of the first and second entities, for example, by determining the positions of the images of these entities, and in some cases, with sub-diffraction limit resolution. In some cases, the z positions may be determined using one of a variety of techniques that uses intensity information or focal information (e.g., a lack of focus) to determine the z position. Non-limiting examples of such techniques include astigmatism imaging, off-focus imaging, or multi-focal-plane imaging. Other aspects of the invention relate to systems for sub-diffraction limit image resolution, computer programs and techniques for sub-diffraction limit image resolution, methods for promoting sub-diffraction limit image resolution, and the like. 177-. (canceled)78. A microscope apparatus comprising:an illumination system that irradiates light to activate and excite a fraction of a plurality of entities in a sample;an imaging ...

Reduced Dimensionality Structured Illumination Microscopy With Patterned Arrays of Nanowells

Techniques are described for reducing the number of angles needed in structured illumination imaging of biological samples through the use of patterned flowcells, where nanowells of the patterned flowcells are arranged in, e.g., a square array, or an asymmetrical array. Accordingly, the number of images needed to resolve details of the biological samples is reduced. Techniques are also described for combining structured illumination imaging with line scanning using the patterned flowcells.

PORTABLE ULTRAVIOLET EXCITED FLUORESCENCE INTENSITY DETECTOR

The present application discloses a portable ultraviolet excited fluorescence intensity detector for carrying out quantitative fluorescence detection for a disposable hygiene product's raw materials and a method of using the same, comprising a housing, an ultraviolet emitting light path, a first receiving light path and a second receiving light path 1. A portable ultraviolet excited fluorescence intensity detector for carrying out quantitative fluorescence detection for a disposable hygiene product's raw materials , comprising a housing , wherein a detection opening is formed in a planar sidewall of the housing in which an ultraviolet emitting light path , a first receiving light path and a second receiving light path are defined such that these light paths are coplanar ,wherein after the portable ultraviolet excited fluorescence intensity detector is placed on a planar substrate to be tested, the ultraviolet emitting, first receiving and second receiving light paths converge at a point in a surface of the planar substrate,wherein an ultraviolet source is arranged in the ultraviolet emitting light path and is adapted to emit ultraviolet light towards the point and excite fluorescence there, a first sensor and a second sensor are arranged in the first receiving light path and the second receiving light path respectively such that the sensors can be selectively operated,wherein the first sensor is designed to convert light, whose wavelength is between 420 and 480 nm, into electric signals proportional to the light intensity, the second sensor is designed to convert light, whose wavelength is between 480 and 760 nm, into electric signals proportional to the light intensity, andwherein a first filter device and a second filter device are allocated to the first and second sensors respectively such that the excited fluorescence can be received by the first and second sensors through the first and second filter devices respectively.2. The portable ultraviolet excited ...

WAVELENGTH SCANNING APPARATUS AND METHOD OF USE THEREOF

A wavelength scanning apparatus that detects at least four different fluorescent emission wavelengths simultaneously or nearly simultaneously is described. The wavelength scanning apparatus includes a heating block having at least four sample wells, each sample well configured for receiving a sample, at least four excitation activation apertures, and at least four fluorescence emission discharge apertures. The wavelength scanning apparatus also includes an analysis scanner having at least four light sources, where the at least four light sources excite at least four fluorophores, at least four excitation light filters that filter out light except that of the desired excitation wavelength/s, at least four fluorescence emission light filters that filter out light except that of the desired fluorescent emission wavelengths, and at least four photodetectors to detect light of the desired fluorescent emission wavelengths. 1. A wavelength scanning apparatus for measuring fluorescence emission of a plurality of nucleic acid samples , comprising: 'the reset button is in mechanical communication with the reset trigger;', 'a reset button and a reset trigger, wherein'}, 'a structural support, the structural support comprising'} [ 'each of the plurality of sample wells forms an opening on a top side of the heating block,', 'a plurality of sample wells, wherein'}, 'a plurality of excitation activation apertures, and', 'a plurality of fluorescence discharge apertures, wherein', 'each of the plurality of sample wells transitions to a corresponding excitation activation aperture of the plurality of excitation activation apertures and a corresponding fluorescence discharge aperture of the plurality of excitation activation apertures, and wherein', 'each of the plurality of excitation activation apertures forms an opening on a bottom side of the heating block and each of the plurality of fluorescence discharge apertures forms an opening on a front side of the heating block;, 'a ...

Microfluidic system for amplifying and detecting polynucleotides in parallel

The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.

METHODS AND SYSTEMS FOR INCORPORATING BIO-SENSORS IN DRONES TO WIRELESSLY DETECT BIOLOGICAL MOLECULES AND HAZARDS

Exemplary methods and systems for incorporating bio-sensors in drones to wirelessly detect biological molecules and hazards without exposing an operator to harmful contaminants or conditions. Bio-sensors can incorporate simultaneous dual-detection methods to ensure accuracy of measurements. Methods of operation include registering blank and safe air profiles for comparison against unknown air profiles to accurately determine the presence of bio-contaminants in unknown air. 1. A drone-sensor system comprising: a system controller comprising a processor, an input/output (I/O) system, memory, and a first power supply', 'a wireless transmitter electronically coupled to the system controller, wherein the wireless transmitter is configured to receive a plurality of instructions from a remote control station and transfer the plurality of instruction to flight control systems, flight sensor systems, and a bio-sensor;', 'the flight control systems electronically coupled to the system controller, wherein the flight control systems maneuver the UAV based on instructions received from; and', 'flight sensor systems comprising a video-camera system and an orientation sensor, wherein the flight sensor systems are electronically coupled to the system controller;, 'an unmanned aerial vehicle (UAV) comprising a sampling chamber comprising a cell forming a first cavity section, a first and second aperture on a first and second end, respectively, a third and four aperture, a reflective filter, and a reflective coating;', 'a laser diode configured to generate and direct a laser beam at a first wavelength through the third aperture into the sampling chamber and through the fourth aperture out of the sampling chamber, wherein the reflective filter is reflective to the first wavelength, wherein as a first optical path of the laser beam passes through the sampling chamber, the first optical path reflects off of the reflective coating and reflective filter at least once each;', 'a first ...

Integrated device with external light source for probing detecting and analyzing molecules

System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes at least one waveguide configured to propagate excitation energy to the sample wells from a region of the integrated device configured to couple with an excitation energy source. A pixel may also include at least one element for directing the emission energy towards a sensor within the pixel. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device. One of multiple markers distinguishable by temporal parameters of the emission energy may label the sample and configuration of the sensor within a pixel may allow for detection of a temporal parameter associated with the marker labeling the sample.

Systems and methods for serial flow emulsion processes

Disclosed herein are systems and methods for serial flow emulsion processes. Systems and methods as described herein result in reduced cross-contamination.

MULTI-CHANNEL FLUORESCENCE DETECTION DEVICE

The multi-channel fluorescence detection device includes an illumination module, plural heating chambers, a detection module and a transmission module. The illumination module includes at least one light source, plural different types of excitation filters, and a first rotational drum, wherein the light source provides a broad band illumination, each of the excitation filters passes light at a particular band width for exciting a targeted fluorescent probe, and the first rotational drum drives the excitation filters. The plural heating chambers are adapted for accommodating PCR tubes having samples and the targeted fluorescent probes. The detection module includes plural different types of emission filters, a second rotational drum and at least one photo-detector, wherein each of the emission filters passes light at a particular band width, the second rotational drum drives the emission filters, and the photo-detector receives fluorescent signals and converts the fluorescent signals to electrical signals. The transmission module includes an actuator connecting with the first and the second rotational drums to drive rotations of the first and the second rotational drum simultaneously for switching and synchronizing the excitation filters and the emission filters to match specific wavelengths of the targeted fluorescent probes. 1. A multi-channel fluorescence detection device , comprising:an illumination module including at least one light source, plural different types of excitation filters, and a first rotational drum, wherein the light source provides a broad band illumination, each of the excitation filters passes light at a particular band width for exciting a targeted fluorescent probe, and the first rotational drum drives the excitation filters;plural heating chambers adapted for accommodating PCR tubes having samples and the targeted fluorescent probes;a detection module including plural different types of emission filters, a second rotational drum and at ...

SYSTEM AND METHOD OF USING MULTI-CHAMBERED RECEPTACLES

A receptacle comprises opposed members, a plurality of chambers having perimeter walls defined by seals formed between the opposed members and portals interconnecting the chambers, and a rigid frame supporting the opposed members at their peripheral edges. The frame comprises a front frame portion and a rear frame portion, and the peripheral edges of the opposed members are retained between the front and rear frame portions. An inlet port extends between the front and rear frame portions and is in fluid communication with one of the chambers. 1. A receptacle comprising:opposed members;a plurality of chambers having perimeter walls defined by seals formed between the opposed members and portals interconnecting the chambers;a rigid frame supporting the opposed members at their peripheral edges, wherein the frame comprises a front frame portion and a rear frame portion, and wherein the peripheral edges of the opposed members are retained between the front and rear frame portions; andan inlet port that extends between the front and rear frame portions and is in fluid communication with one of the chambers.2. The receptacle of claim 1 , further including a port cover configured to close off the inlet port.3. The receptacle of claim 1 , wherein the frame comprises an identifying label.4. The receptacle of claim 1 , wherein the identifying label comprises a bar code.5. The receptacle of claim 1 , wherein the frame comprises a tab projecting from the frame to provide an appendage for grasping the receptacle.6. The receptacle of claim 5 , wherein the inlet port extends through the tab.7. The receptacle of claim 1 , wherein the frame comprises a front frame component and a rear frame component.8. The receptacle of claim 7 , wherein the front frame component and the rear frame component are injection molded and are connected to one another by ultrasonic welding.9. The receptacle of claim 7 , wherein the front frame component and the rear frame component are rectangular claim 7 ...

SYSTEM AND METHOD OF USING MULTI-CHAMBERED RECEPTACLES

A method of processing a sample in a receptacle comprising a plurality of chambers. Each of the chambers is connected to at least one other chamber by a portal and at least a first one of the chambers is formed of a flexible material. The method includes the steps of causing gas bubbles contained in the first chamber to accumulate in a portion of the first chamber, applying a compressive external force to the first chamber to cause some or all of the liquid contents of the first chamber to flow into an interconnected second chamber through a portal connecting the first and second chambers; and preventing the gas bubbles accumulated in a portion of the first chamber from flowing through the portal into the second chamber 1. A method of processing a sample in a receptacle comprising a plurality of chambers , wherein each of the chambers is connected to at least one other chamber by a portal and at least a first one of the chambers is formed of a flexible material , the method comprising:A. causing gas bubbles contained in the first chamber to accumulate in a portion of the first chamber;B. applying a compressive external force to the first chamber to cause some or all of the liquid contents of the first chamber to flow into an interconnected second chamber through a portal connecting the first and second chambers; andC. preventing the gas bubbles accumulated in a portion of the first chamber from flowing through the portal into the second chamber during step B.2. The method of claim 1 , wherein the gas bubbles are prevented from flowing through the portal into the second chamber during step B by a wall extending from a peripheral boundary of the first chamber into an interior space of the first chamber.3. The method of claim 1 , wherein causing the gas bubbles to accumulate in a portion of the first chamber comprises orienting the receptacle so that the gas bubbles accumulate in an upper portion of the first chamber.4. The method of claim 1 , wherein the receptacle ...

METHOD AND SYSTEM FOR ITEM AUTHENTICATION AND CUSTOMIZATION

A method of illuminating an item is disclosed. The method includes applying adhesive to the item, interspersing a taggant in the adhesive, illuminating the item with an excitation signal, sensing luminescence emitted by the taggant in response to illumination by the excitation signal, and determining the authenticity of the item based on the sensed emitted luminescence. The item can include any item benefited by authentication, and can include a postage stamp. A method of customizing an item is disclosed. This can include the steps of preparing a substrate, applying a security feature to the substrate, printing non-customized information on the substrate, receiving image information, and printing the image information on the substrate. 1. A method of authenticating an item , the method comprising:applying an adhesive to a surface of the item, the adhesive comprising a first taggant and second taggant;applying a second adhesive in a second adhesive area, the second adhesive comprising the second taggant;illuminating the item with an excitation signal, wherein the first taggant and second taggant emit luminescence in response to the excitation signal, the emitted luminescence comprising a desired image;sensing the emitted luminescence from the taggant; anddetermining the authenticity of the item based on the sensed emitted luminescence having the desired image.2. The method of claim 1 , wherein the excitation signal comprises electromagnetic energy within a predetermined spectrum.3. The method of claim 1 , wherein the emitted luminescence comprises electromagnetic energy within a predetermined spectrum.4. The method of wherein the taggant comprises two or more taggants which emit different wavelengths of electromagnetic energy when excited by the excitation signal.5. A method of authenticating an item through a substrate claim 1 , the method comprising:applying a taggant to a substrate of the item, the taggant configured to emit luminescence in response to ...

System and method for detecting multiple-excitation-induced light in a flow channel

A system for detecting signal components of light induced by multiple excitation sources, which includes a flow channel having two spatially separated optical interrogation zones; a light illumination subsystem that directs each of two light beams of different wavelengths to a different zone of the optical interrogation zones; a detector subsystem that converts detected light into a total electrical signal having both modulated and unmodulated electrical signals; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

TWO-DIMENSIONAL FLUORESCENCE DIFFERENCE SPECTROSCOPY CHARACTERIZATION OF NANOPARTICLES AND THEIR INTERACTIONS

Methods of characterizing nanoparticles, nanoparticle complexes, and their biomolecular interactions are provided. Concurrent excitation and emission wavelength scans are performed and the optimal fluorescence intensity for the intersect of these wavelengths is determined. The intersect is dependent upon the physical characteristics of the particle and can be used to verify, for example, attachment of biomolecules, amount of biomolecules present, and structure of the attached biomolecule. 1. A method of detecting the attachment of an organic species onto the surface of one or more nanoparticles within a sample comprising the nanoparticles and the organic species , wherein the method comprises performing concurrent scans of the fluorescence excitation and emission wavelengths for the nanoparticles within the sample and determining an intersect between the excitation and emission wavelengths yielding the highest fluorescence intensity , the intersect indicating the presence or absence within the sample of a nanoparticle complex comprising the one or more nanoparticles having the organic species attached to the surface of the nanoparticle.2. The method of claim 1 , wherein the method further comprises comparing the intersect to one or more known standards for the nanoparticle and/or nanoparticle complex.3. The method of claim 2 , wherein the one or more known standards comprise the intersect between the excitation and emission wavelengths yielding the highest intensity for the nanoparticle without the organic species attached to the surface of the nanoparticle.4. The method of claim 1 , wherein the organic species comprises a member selected from the group consisting of proteins claim 1 , protein enzymes claim 1 , RNA claim 1 , DNA claim 1 , oligonucleotides claim 1 , aptamers claim 1 , pharmaceutical compounds claim 1 , and combinations thereof.5. The method of claim 4 , wherein the organic species comprises a member selected from the group consisting of B- ...

SCANNING REAL-TIME MICROFLUIDIC THERMOCYCLER AND METHODS FOR SYNCHRONIZED THERMOCYCLING AND SCANNING OPTICAL DETECTION

Systems and methods for performing simultaneous nucleic acid amplification and detection. The systems and methods comprise methods for managing a plurality of protocols in conjunction with directing a sensor array across each of a plurality of reaction chambers. In certain embodiments, the protocols comprise thermocycling profiles and the methods may introduce offsets and duration extensions into the thermocycling profiles to achieve more efficient detection behavior. 114-. (canceled)15. A method implemented on one or more computer processors for optimizing protocols for simultaneously performing a plurality of thermal cycling reactions , wherein each thermal cycling reaction comprises one or more detection steps , and wherein the thermal cycling reactions are performed in a plurality of reactors using a plurality of heating elements in thermal communication with the plurality of reactors and a detector head , the method comprising:determining or providing or accessing a detection cycle time for each of the plurality of reactors;receiving or accessing a protocol step of a protocol, the step associated with a step duration, the step comprising a time for detection;determining a first adjustment to the step such that the step duration is a multiple of the detection cycle time; andcontrolling the detector head and at least one of the plurality of heating elements to perform the protocol modified to include the first adjustment to the step.16. The method of claim 15 , further comprising determining a second adjustment to the step such that the time for detection occurs at a multiple of the detection cycle time when the step is adjusted by the first adjustment and by the second adjustment.17. The method of claim 15 , further comprising determining a starting offset adjustment based on a position of the reactor associated with the protocol.18. The method of claim 15 , wherein the detection cycle time comprises the amount of time required for the detector head to perform a ...

AUTOMATED METHOD FOR DETECTING CERVICAL CANCERS AND HIGH GRADE HYPERPLASIAS

Automated methods for detecting cancer and related hyperplasias in biological samples. 1. An automated method of screening for the presence and/or extent of a pathology in a subject , the pathology characterized by an abnormal chromosomal component in a cell of the subject , comprising the steps ofa) contacting a biological sample comprising cell nuclei from said subject with one or more distinguishable labeled probes directed to at least one chromosomal sequence that characterizes the abnormality under conditions that promote hybridization of the one or more probes to the at least one sequence;b) causing interrogatable information regarding said one or more distinguishable labeled probes to be associated with said biological sample; irradiating said biological sample with fluorescence exciting illumination based on said interrogatable information,', 'capturing one or more images of said biological sample,', 'digitizing each of said one or more images,', 'analyzing at least one of said one or more digitizations to cause adjustment of exposure parameters to form said digitized representation;, 'c) automatically obtaining a digitized representation of the one or more distinguishable labels hybridized to the chromosomal sequences by'}d) automatically analyzing the distribution and intensity of binding of the one or more distinguishable labels in the digitized representation to determine the presence and/or extent of an abnormal chromosomal component; ande) automatically reporting results of the analysis of step d); wherein steps b)-d) are carried out without intervention by a human.2. The method described in wherein an automated microscope system carries out steps b)-e).3. The method described in wherein a probe targets at least one of a single nucleotide polymorphism (SNP) claim 1 , a mutated sequence claim 1 , a duplicated or amplified gene or portion thereof claim 1 , a centromere of chromosome 3 claim 1 , a centromere of chromosome 7 claim 1 , and a sequence ...

CANCER CELL DETECTION AND IMAGING SYSTEM, PROCESS AND PRODUCT

A method of distinguishing cancerous cells and healthy cells of a subject from each other comprises the steps: (i) contacting a region of tissue of a subject suspected of including at least some cancer cells with a plurality of nanodiamonds, wherein the plurality of nanodiamonds comprise a first plurality of conjugates, wherein the conjugates of the first plurality of conjugates consist of first nanodiamonds and one or more cancer cell targeting agents, wherein the first nanodiamonds have a first type of colour center, and a second plurality of conjugates, wherein the conjugates of the second plurality of conjugates consist of second nanodiamonds and one or more healthy cell targeting agents, wherein the second nanodiamonds have a second type of colour center; and (ii) applying light of a first wavelength so as to excite the first type of colour center and applying light of a second wavelength so as to excite the second type of colour center, wherein upon contacting the region of tissue with the plurality of nanodiamonds, cancer cells are adhered to the first plurality of conjugates, and healthy cells are adhered to the second plurality of conjugates; wherein upon applying light to the region of tissue, the colour centers of the nanodiamonds of the first plurality of conjugates adhered to cancer cells emit fluorescence at a first wavelength, and the colour centers of the nanodiamonds of the second plurality of conjugates adhered to healthy cells emit fluorescence at a second wavelength; and wherein the colour contrast between the two wavelengths and the positions of respective conjugates delineate the area of cancer cells and the area of healthy cells from each other. 1. A method of distinguishing cancerous cells and healthy cells of a subject from each other , said method including the steps of:contacting a region of tissue of a subject suspected of including at least some cancer cells with a plurality of nanodiamonds, wherein said plurality of nanodiamonds ...

PORTABLE DEVICE FOR CONTROLLING AN ALCOHOLIC BEVERAGE THROUGH A CONTAINER, A SYSTEM AND A METHOD ASSOCIATED THERETO

The present disclosure provides a portable device for controlling an alcoholic beverage that includes a single light source emitting a monochromatic excitation light beam of a wavelength between 350 and 650 nanometers, a beam splitter to reflect the light beam, a focus and collection lens, and a positioning device to orient the light beam along a direction substantially normal to an outer surface of the container. The positioning device positions the outer surface of the container at a predetermined distance from the focus lens. The device includes a filtering device for filtering fluorescence radiation captured by the focus lens to eliminate wavelengths shorter than or equal to the wavelength of the light beam. A spectrometer module produces a signal corresponding to the measured spectrum of the fluorescence radiation and an analysis module compares the measured spectrum to a reference spectrum. 1. A portable device for controlling an alcoholic beverage in an at least partially transparent container , the portable device comprising:a single light source emitting a monochromatic excitation light beam having a wavelength between 350 and 650 nanometers;a beam splitter oriented at 45° with respect to the direction of emission of the light source to reflect the excitation light beam;a focus and collection lens;a positioning device that orients the excitation light beam emitted from the light source along a direction normal to an outer surface of the container, wherein the positioning device is operable to position the outer surface of the container at a predetermined distance from the focus and collection lens such that the excitation light beam is focused within the container and the distance from the wall of the container is less than 1 millimeter, wherein the positioning device includes a through opening for passage of the excitation light beam;at least one filtering device for filtering fluorescence radiation captured by the focus lens and transmitted by the beam ...

Biosensor for Conformation and Secondary Structure Analysis

The invention provides an infrared detection system for conformation and secondary structure analysis, notably for the direct non-invasive qualitative secondary structure analysis of a single selected protein within a complex mixture, as e.g. a body fluid, by vibrational spectroscopic methods utilizing a quantum-cascade laser. For the analysis it is not required that the selected substance be isolated, concentrated, or pretreated by a special preparative procedure. A difference-spectrum between the unbound and antibody-bound protein of interest is performed by which the much larger background absorbance is cancelled. The presented quantum-cascade laser set-up provides sufficient S/N and stability to subtract the several orders of magnitude larger background absorbance. 1. An infrared detection system comprising:(a) a tunable quantum-cascade laser as an infrared (IR) light source, and a germanium internal reflection element comprising a trapezoid or parallelogram shape, which shape is configured to provide for more than one passage of infrared light from the IR light source through the reflection element, and being transparent in infrared light with sufficient signal to noise ratio to detect an amide I band of a candidate biomarker protein, and', 'at least one antibody capable of specific and conformationally independent binding to the candidate biomarker protein, wherein the antibody is covalently attached to at least one surface of said internal germanium reflection element., '(b) an IR cell with an infrared sensor element that comprises'}2. The infrared detection system of claim 1 , wherein(i) the sensor element is further suitable for parallel analysis by another optical method; and/or(ii) the internal reflection element is a germanium monocrystal.34.-. (canceled)5. The infrared detection system of claim 1 , wherein the candidate biomarker protein is an amyloidogenic peptide.6. The infrared detection system of claim 1 , wherein said system further comprises an IR ...

Spectrum measuring device and spectrum measuring method

A spectrum measuring device according to the present disclosure includes an accommodation part having a space for accommodating a culture solution containing a medium and cells, a light source device for irradiating a first surface of the space with excitation light, a light receiving device for receiving fluorescence occurring from the first surface, and an arithmetic device for spectral analysis of the fluorescence received by the light receiving device. In the space, the depth from the first surface of a fluorescence region where fluorescence is produced by incident excitation light is a value giving an internal shield of a prescribed level of less in the fluorescence region. The arithmetic device calculates the fluorescence spectrum of the cells by subtracting the fluorescence spectrum of the medium from the spectrum of the fluorescence of the culture solution obtained by the light receiving device.

Particle detecting device and particle detecting method

A particle detecting device includes: a light source that illuminates a fluid with an excitation beam; a fluorescent intensity measuring instrument that measures an optical intensity of a fluorescent band, generated in a region that is illuminated by the excitation beam, at two or more wavelengths; an evaluating portion that compares a measured value for the optical intensity and a boundary range, which is a range of optical intensities at two or more wavelengths for discriminating between a fluorescent particle that is a subject to be detected and a particle that is not a subject to be detected, and evaluates whether or not a fluid includes a fluorescent particle that is a subject to be detected; and a correcting portion that corrects the boundary range in accordance with the status of at least one of the light source and the fluorescent intensity measuring instrument.

DETECTION METHOD AND DETECTION SYSTEM USING SUSPENSION LIQUID BIOCHIP

Disclosed are a detection method and detection system using suspension liquid biochip detection. The detection method using suspension liquid biochip comprises the following steps: an excitation step and a signal acquisition step. In the excitation step, a laser light emitted by a single laser device is used to excite a classification fluorescence of nanocrystalline fluorescent microspheres and a report fluorescence of an object to be detected; and in the signal acquisition step, fluorescence detection signals are obtained through multiple acquisition channels, respectively. In the present invention, the classification fluorescence in the nanocrystalline fluorescent microspheres and the report fluorescence in the object to be detected in a sample are excited by a single laser device, which is convenient for operation and reduces the production cost of the system, thereby reducing the detection cost. 112-. (canceled)13. A detection system using suspension liquid biochip , characterized in that a housing; the housing encloses a chamber; a clamp is installed on the housing , and the clamp is used to clamp a tube; the clamp is rotatably arranged on the housing , and the clamp is provided with a first clamping hole and a second clamping hole; the first clamping hole is used for a first tube inserting , and the second clamping hole is used for a second tube inserting; an axis of the first clamping hole intersects an axis of the second clamping hole; the clamp is rotatably arranged , and when the clamp rotates , an opening of the first tube or an opening of the second tube faces a direction suitable for use.14. The detection system using suspension liquid biochip according to claim 13 , characterized in that the axis of the first clamping hole is perpendicular to the axis of the second clamping hole.15. The detection system using suspension liquid biochip according to claim 13 , characterized in that the clamp includes a base and a plurality of clamping jaws claim 13 , and ...