АВТОМАТИЧЕСКАЯ СИСТЕМА И СПОСОБ ОБНАРУЖЕНИЯ ТОЧЕЧНОГО ИСТОЧНИКА БИОЛОГИЧЕСКОГО АГЕНТА

Изобретение относится к системам обнаружения биологической опасности, в особенности к системе обнаружения таких биологически опасных агентов, как возбудитель сибирской язвы, в почтовых отправлениях. Полностью автоматизированная система обнаружения биологических агентов содержит устройство для идентификации биологического агента, собирающее устройство для отбора аэрозольной пробы частиц распыленного биологического агента на одном участке контроля и устройство для концентрирования аэрозолей. Устройство для концентрирования аэрозолей предназначено для получения жидкой пробы из аэрозольной пробы. Автоматизированная система имеет автоматическое струйное устройство для хранения и переноса порции жидкой пробы в контейнер типа картриджа, автоматическую механическую систему перемещения контейнера из зоны накопления в пункт заливки жидкости струйного устройства, а затем в устройство для идентификации биологического агента. Устройство для идентификации биологического агента включает идентификатор ...

ГИБКИЙ КОНТЕЙНЕР ДЛЯ ОБРАЗЦА

Группа изобретений относится к системе для удержания образца текучего вещества при проведении измерения и способу подачи образца текучего вещества в оптический сканирующий аппарат. Система содержит прозрачную гибкую трубку для удержания образца текучего вещества, держатель трубки для удержания трубки, первый и второй расплющивающие элементы. При этом первый и второй расплющивающие элементы можно перемещать относительно друг друга, тем самым изменяя первое состояние прозрачной гибкой трубки на второе состояние, где по меньшей мере первый размер в поперечном сечении трубки меньше во втором состоянии, чем в первом состоянии. Система дополнительно содержит оптический сканирующий аппарат, содержащий устройство регистрации изображений для получения изображений образца текучего вещества, содержащегося в гибкой трубке. Заявленная группа изобретений позволяет обеспечить более простой и качественный анализ образца текучего вещества. 2 н. и 13 з.п. ф-лы, 8 ил.

РЕАКЦИОННЫЙ СОСУД И РЕАКЦИОННОЕ УСТРОЙСТВО, ПРЕДНАЗНАЧЕННЫЕ ДЛЯ ПРОВЕДЕНИЯ ПОЛИМЕРАЗНОЙ ЦЕПНОЙ РЕАКЦИИ

Группа изобретений относится к области биотехнологии. Предложен реакционный сосуд и реакционное устройство для проведения полимеразной цепной реакции. Реакционный сосуд содержит подложку, канал для перемещения образца, отверстие для введения образца внутрь канала, пару воздушных коммуникационных отверстий на концах канала и область термического цикла между парой воздушных коммуникационных отверстий. Причем канал содержит множество каналов ответвления между областью термического цикла и воздушным коммуникационным отверстием, а область термического цикла содержит первую температурную область и вторую температурную область. Реакционное устройство содержит указанный реакционный сосуд, температурный регулятор и систему подачи жидкости. Изобретения обеспечивают надлежащее перемещение образца для проведения устойчивого ПЦР. 2 н. и 2 з.п. ф-лы, 7 ил.

КАРТРИДЖ И УСТРОЙСТВО ДЛЯ ПОДГОТОВКИ БИОЛОГИЧЕСКОГО ОБРАЗЦА

Изобретение относится к картриджу для обработки биологического образца. Картридж содержит корпус с реакционной камерой, системой подачи текучей среды и системой отвода текучей среды. В реакционной камере имеется отверстие, которое может закрываться подложкой, несущей образец. Система подачи текучей среды обеспечивает поступление по меньшей мере одного текучего реагента в реакционную камеру. Система подачи текучей среды, реакционная камера с подложкой, закрывающей ее отверстие, и система отвода текучей среды являются закрытыми для обмена жидкостями с окружающей средой. Подложка может прикрепляться к корпусу картриджа с помощью адгезии, склеивания, вакуумной камеры, прижатия или электромагнитных сил. Высота реакционной камеры над подложкой составляет менее 200 мкм, предпочтительно менее 50 мкм. Обеспечивается закрытость системы, включающей картридж и подложку, от окружающей среды, отсутствие опасности внесения загрязнения в образец из окружающей среды или загрязнения окружающей среды образцом ...

МИКРОСТРУЙНОЕ УСТРОЙСТВО И СПОСОБ ЕГО ИЗГОТОВЛЕНИЯ И СОДЕРЖАЩИЙ ЕГО СЕНСОР

... 1. Микроструйное устройство, содержащее: ! - первую подложку (120), имеющую первую поверхность (122); ! - вторую подложку (128), имеющую вторую поверхность (126); ! причем вторая поверхность обращена к первой поверхности, и между первой и второй подложкой образована граница раздела; ! - первые вырезы (124) и вторые вырезы (130), предусмотренные на границе раздела; ! при этом первые вырезы и вторые вырезы образуют канал (104), меандрирующий в плоскости под прямыми углами к границе раздела; и ! по меньшей мере часть вырезов содержит пористый материал (114), ! отличающееся тем, что вторая поверхность контактирует с первой поверхностью, образуя при этом указанную границу раздела между первой и второй подложкой. ! 2. Микроструйное устройство по п.1, в котором первые вырезы (124) расположены в первой поверхности (122), а вторые вырезы (130) расположены во второй поверхности (126). ! 3. Микроструйное устройство по п.1, в котором по меньшей мере часть вырезов (124, 130) заполнена другим пористым ...

УСТРОЙСТВО ДЛЯ АНАЛИЗОВ И СПОСОБ ВЫПОЛНЕНИЯ БИОЛОГИЧЕСКИХ АНАЛИЗОВ

... 1. Анализирующее устройство, содержащее реакционное пространство и, по меньшей мере, два набора индивидуально закодированных микроносителей (2), в которомреакционное пространство является микроканалом (1);при этом микроносители (2) имеют форму относительно сечения микроканала (1), которая позволяет иметь по всей длине микроканала (1), по меньшей мере, два каких-либо микроносителя (2), расположенных бок о бок без соприкосновения друг с другом и без соприкосновения с периметром микроканала (1);при этом устройство (1) содержит средство (4) для ограничения перемещения микроносителей (2) в продольном направлении микроканала (1), наряду с тем, что жидкости все еще могут протекать; акод микроносителей является указывающим на функцию.2. Анализирующее устройство по п.1, в котором микроканал (1) прозрачен, по меньшей мере, с одной стороны и/или, по меньшей мере, на одном участке есть возможность оптического наблюдения за микроносителями (2).3. Анализирующее устройство, по п.1, в котором сечение микроканала ...

АМПЛИФИКАЦИЯ БИБЛИОТЕК НУКЛЕИНОВЫХ КИСЛОТ С ПРИМЕНЕНИЕМ КИНЕТИЧЕСКОГО ИСКЛЮЧЕНИЯ

ВРАЩАЮЩЕЕСЯ ДИСКООБРАЗНОЕ УСТРОЙСТВО ДЛЯ СБОРА ОБРАЗЦОВ ТЕКУЧЕЙ СРЕДЫ

... 1. Устройство для сбора образцов текучей среды, содержащее:по существу дискообразный корпус, имеющий периферию;проходящий через корпус и ограниченный периферией капиллярный канал, имеющий первый конец и второй конец, причем первый конец выполнен с возможностью втягивания текучей среды в канал с помощью капиллярного эффекта;резервуар для сбора образцов, размещенный вблизи второго конца и сообщающийся по текучей среде с капиллярным каналом; иось вращения, проходящую через центр диска и по существу перпендикулярную основной поверхности дискообразного корпуса.2. Устройство для сбора образцов по п. 1, в котором устройство для сбора образцов выполнено с возможностью вращения вокруг оси вращения внутри кассеты, корпус которой содержит воздушное отверстие, сообщающееся по текучей среде с капиллярным каналом во время вращения диска в первое положение.3. Устройство для сбора образцов по п. 2, в котором воздушное отверстие размещено вблизи второго конца капиллярного канала и сообщается с ним по текучей ...

Mikrofluidische Glykananalyse

Es werden Mikrofluideinheiten und -verfahren zum Analysieren der Glykanprofile von Glykoproteinen bereitgestellt. Einige Ausführungsformen der Mikrofluideinheiten weisen eine Deglykosylierungs-Säule zum Abspalten der Glykane, wahlweise eine Reinigungssäule zum Entfernen der Proteine, eine Trap-Säule zum Anreichern der Glykane und eine Trennsäule zum Trennen der Glykane auf. Die Mikrofluideinheiten und -verfahren verbessern die Geschwindigkeit und das Auflösungsvermögen der Glykananalyse beträchtlich.

Vorrichtung zur Aufnahme von Tierzellenkolonien

Vorrichtung zum Aufnehmen von Tierzellkolonien, welche folgendes umfasst: - ein Vorrichtungsbett (10) zum Anordnen eines Probenbehälters (13), welcher eine Mehrzahl von in einem Medium (72) gehaltenen Tierzellkolonien (74) umfasst, - eine Kamera (40) zum Aufnehmen von Bildern der Tierzellkolonien (74), - einen Bildprozessor (90) zum Identifizieren von Stellen von Tierzellkolonien (74) anhand von aufgenommenen Bildern - und einen Aufnahmekopf (18), der unter Verwendung von Positionierungsmotoren (20, 22, 24) relativ zu dem Vorrichtungsbett zu Stellen von Tierzellkolonien (74), die durch den Bildprozessor (90) identifiziert wurden, bewegt werden kann, - wobei der Aufnahmekopf (18) eine Mehrzahl von hohlen Nadeln (26) beinhaltet, die durch Fluidleitungen (28) mit einer Drucksteuerung (84) verbunden sind, - die so betreibbar ist, dass sie Mengen des Mediums (72) aus dem Probenbehälter (13) in die hohlen Nadeln (26) einsaugt, das Medium (72) hält und es ausstößt, wenn dies erforderlich ist, ...

PNEUMATISCHE STEUERUNG DER BILDUNG UND DES TRANSPORTS VON FLÜSSIGPROBEN KLEINEN VOLUMENS

Blasen eliminierende Struktur, Blasen eliminierendes Verfahren und Umwälzverfahren, das selbige nutzt

Vorgesehen werden eine Blasen eliminierende Struktur und ein Blasen eliminierendes Verfahren, welche Blasen in einer Flüssigkeit durch Umwälzen der Flüssigkeit eliminieren, und ein Umwälzverfahren, das die selbigen nutzt. Eine erste Rille (114), welche eine stromaufwärtige, Blasen eliminierende Rille ist, und eine zweite Rille (131), welche eine stromabwärtige, Blasen eliminierende Rille ist, sind von einer Mischvertiefung (13) abgezweigt. Nach Starten einer Ansaugung der Flüssigkeit von der Mischvertiefung (13) in die erste Rille (114) wird eine Ansaugung der Flüssigkeit von der Mischvertiefung (13) in die zweite Rille (131) gestartet, und nach Beendigung einer Ableitung der Flüssigkeit von der ersten Rille (114) in die Mischvertiefung (13) wird eine Ableitung der Flüssigkeit von der zweiten Rille (131) in die Mischvertiefung (13) beendet. Diese Operation wird wiederholt, um Blasen zu eliminieren.

Vorrichtung zum Probeneintrag in ein mikrofluidisches System

Es wird eine Vorrichtung zum Probeneintrag in ein mikrofluidisches System bereitgestellt, umfassend eine Adapterkomponente mit einer oberen Fläche und senkrecht dazu angeordneten Seitenflächen, wobei die Adapterkomponente eine erste und eine zweite Hohlnadel aufweist, welche sich von der oberen Fläche der Adapterkomponente erstrecken, wobei die erste und zweite Hohlnadel jeweils mit einem innerhalb der Adapterkomponente zu einer ersten bzw. zweiten Seitenfläche der Adapterkomponente verlaufenden Kanal in fluider Verbindung stehen, wobei die sich von der oberen Fläche erstreckenden Hohlnadeln gemeinsam von einem Kragen umgeben sind, welcher sich höher als die Hohlnadeln von der oberen Fläche erstreckt, und wobei die Adapterkomponente über den ersten und zweiten Kanal an das mikrofluidische System koppelbar ist, und der Kragen zur Aufnahme einer Probenentnahmeeinrichtung ausgebildet ist, wobei die zu entnehmende Probe über die erste und/oder zweite Hohlnadel aus der Probenentnahmeeinrichtung ...

Mikrofluidische Vorrichtung und Verfahren zur Temperaturregelung eines Arbeitsfluids

Die Erfindung betrifft eine mikrofluidische Vorrichtung (100) umfassend einen ersten Fluidkanal (131) zum Befördern eines ersten Wärmeträgerfluids (121), wobei der erste Fluidkanal (131) mit einer ersten Schnittstelle (141) zum Wärmeaustausch zwischen dem ersten Wärmeträgerfluid (121) und einem Arbeitsfluid (151) verbunden ist, wobei die erste Schnittstelle (141) einen Hohlraum in der Vorrichtung (100) umfasst und mit einem ersten Arbeitsfluidkanal (161) zum Leiten von Arbeitsfluid (151) zur erste Schnittstelle (141) verbunden ist. Ferner betrifft die Erfindung ein Verfahren (500) zum Betrieb der mikrofluidischen Vorrichtung (100).

VORRICHTUNG ZUR UEBERTRAGUNG VON BIOLOGISCHEN FLUIDEN UND REAGENZMISCHUNGEN

Vorrichtung zum Einbringen einer flüssigen Probe in ein mikrofluidisches System

Die Erfindung betrifft eine Vorrichtung zum Einbringen einer flüssigen Probe in ein mikrofluidisches System, umfassend eine Adapterkomponente 10, welche über zumindest einen Kanal 20 an das mikrofluidische System fluidisch koppelbar ist, und welche einen Aufnahmeabschnitt 30 aufweist, wobei der Aufnahmeabschnitt 30 zur Führung und Aufnahme eines Probenbehälters 40 vorgesehen ist, und den Probenbehälter 40 mittels eines Begrenzungselements 50 nach dem Einführen des Probenbehälters 40 in dem Aufnahmeabschnitt 30 in einer ersten vorbestimmten Aufnahmeposition positioniert, und der Probenbehälter 40 ausgehend von der ersten vorbestimmten Aufnahmeposition bis zu einer zweiten vorbestimmten Aufnahmeposition innerhalb des Aufnahmeabschnitts 30 überführt wird, in welcher ein unterhalb des Aufnahmeabschnitts 30 in der Adapterkomponente 10 angeordnetes Öffnungselement 60 den Probenbehälter 40 derart durchdringt, dass die flüssige Probe des Probenbehälters 40 in den zumindest einen Kanal 20 überführbar ...

Mikrofluidik-Einrichtung

Zur Überwachung der Abnutzung einer Mikrofluidik-Einrichtung mit Fluidkanälen (7) sind diesen Sensoren (9) zur Erfassung durchflussrelevante Größen zugeordnet. Ein Einrichtung (13) speichert die Werte der erfassten Größen und eine Vergleichseinrichtung (14) vergleicht die Werte der erfassten Größen mit bereits abgespeicherten früheren Werten. Eine Auswerteeinrichtung (15) erzeugt eine Meldung, wenn die Abweichung zwischen den miteinander verglichenen Werten ein vorgegebenes Maß übersteigt.

Mikrofluidisches System

Methods for detecting target nucleic acids in sample lysate droplets

Low volume chemical and biochemical reaction system

An automated system utilizes an array of nanoscale capillary-dimension reaction chambers (12). The ends of the chambers are temporarily sealed with deformable membranes (264a, 264b) and the reactions effected by incubation or temperature cycling. The reaction containers may be filled by capillary action and dispensed by air displacement, centrifugal force or other means. Reaction mixtures may be assembled by using the reaction chambers (12) to meter reaction components that are combined on a substrate. Alternatively, a first reaction component may be immobilized on the interior surface of the reaction container and a second mixture component pumped of drawn into the container to form a final reaction mixture.

Pipetting appartus

Capillary polymerase chain reaction

A reactor for the polymerase chain reaction (pcr) comprises a capillary, preferably a plurality of capillaries having a common entrance and common exit. A reaction solution is passed therethrough and subject to the steps of melting, primer attachment and amplification (construction) at three different temperatures in either three separate zones or in one zone. The flow rate of the reactants in the construction zone is maintained below ten capillary diameters per second. Preferably, the silicon microfabricated capillaries are coated with conventional chromatographic coatings such a carbowaxes, lipoproteins or glycoproteins and contain high thermal conductivity metal powders such as silver. High frequency AC heating elements combined with cooling plates allow rapid temperature changes; each temperature stage may be passed through in 0.25 of a second. It is envisaged that 30 cycles are completed within 2.5 minutes.

Detectors for microfluidic systems

A method for detecting and measuring samples in the form of liquid droplets or slugs in a carrier liquid comprises providing a microfluidic device with conduits and at least one detector, moving a carrier liquid containing the liquid droplets or slugs through the microfluidic device until a first droplet or slug comes into contact with the at least one detector, measuring at least one property of the liquid droplet or slug with the detector, removing the sample, flushing the detector with a flushing liquid and then repeating the detection sequence. A device for carrying out the method comprises a first conduit, 12 a detector 10 and a second conduit 14 in liquid communication with the first conduit.

Microfluidic device and methods for construction and application

Devices and methods for enrichment and alteration of circulating tumour cells and other particles

The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.

Fluid Multiplexer For Capillary Column Gas Chromatography

A fluid multiplexer 200 includes a manifold 210 having a plurality of fluid conduits 219, 221, 222, 224, 226, 227 formed therein, a fluid input 211 and a plurality of fluid connection points 212, 214, 216, 217, 218 located on the manifold and fluidically coupled together via the fluid conduits, and a flow control module 238 coupled to the fluid connection points, the flow control module configured to provide a plurality of blocking flows 239, 241, 242, 244 to the manifold to control the flow of a primary fluid through 233 the fluid conduits. The multiplexer is for particular use with parallel gas chromatography capillary columns 228, 229, 231, 232, 234 and enables switching between the columns, where blocking flows are applied to all columns except that to be used. Vents, not shown, having flow restrictions may also be coupled to the connection points and the manifold may be of laminar construction.

Fluid flow control in a microfluidic device

The invention relates to controlling a flow of fluids in a microfluidic device (10), the microfluidic device (10) having a fluid channel (140), comprising applying a magnetic field to at least a part of the fluid channel (141, 142) to force at least a portion of a first fluid to retain in at least a portion of the fluid channel (140).

Fluidic cartridge for nucleic acid amplification and detection

Apparatus and method

A microfluidic channel assembly 1 comprising a polymer film 12 secured to the surface of a polymer body10 wherein an elongate groove 16 is formed on the surface of the body and the film and body are at least partially fused together along an elongate track 14 that follows the groove so that the film and track enclose the groove to form the channel. The film and body are formed of the same material with the film having a thickness of less than 1mm and the body having a thickness of at least 2mm. Ideally, the film and body have different optic properties. A detection apparatus comprising a detector, printed circuit board (PCB) and pneumatic interface is disclosed. A second detection apparatus comprising a detector and a pneumatic system comprising a microfluidic channel assembly with a first microfluidic channel for dispensing vapour to the detector wherein the channel comprises a groove in a surface of a polymer body and a film bonded to the surface of the body over the groove wherein the ...

Dipensers

Droplet-Based assay system

Device and apparatus

Droplet-based assay system

A microfluidic system

Bioreactor

The invention concerns a droplet-fluidic bioreactor system and method of use. The method includes introducing a first droplet 6 encapsulated by the carrier medium into the path through the first inlet and a second droplet 7 introduced through a second inlet. The method also includes inducing the first droplet 6 and the second droplet 7 to fuse into a single, third droplet 11. The method also includes inducing the third droplet to split into fourth 12 and fifth 13 droplets and extracting the fourth droplet 12 from the path. The method also includes extracting the fifth droplet 13 through the outlet 4 and re-introducing the fifth droplet 15 into the path 5 through the inlet 3. Preferably the droplets include suspensions of microorganisms and/or cells and suspensions of substances suitable for sustaining the microorganisms and/or cells. Also claimed is a method of preparing a droplet-fluidic bioreactor and a method of fabricating a feature segment for a droplet-fluidic device.

2-dimensional separation

Removing bubbles in a microfluidic device

Improvements in or relating to a fluid flow controller for microfluidic devices

Microfluidic probe head for providing a sequence of separate liquid volumes separated by spacers

Sampling device

Microfluidic device and apparatus

Fluidic device for quantifying the dynamic permeability and hydraulic conductivity of living tissue layers

Breastmilk sample collection

Fluid control delivery device

Microfluidic device with positive displacement pump

A microfluidic device 300 with means for moving fluid through a microfluidic channel of the device, the device comprising a microfluidic channel 301, a positive displacement pump 302 comprising a chamber connected to the channel such that when the pump is actuated fluid within the chamber is displaced into the channel, and a fluid reservoir 307 connected to the pump to provide a source of fluid to re-fill the chamber of the pump after the pump has been actuated wherein the fluid reservoir is arranged such that fluid within the reservoir is sealed within the device. Ideally, the device comprises a first valve 306 positioned between the chamber of the pump and the channel, and a second valve 305 positioned between the chamber of the pump and the fluid reservoir. The device may comprise a continuous fluid loop between the channel and the pump such that there is continuous fluid flow around the device and no negative pressure build up. Ideally, inlet and outlet ports 308, 309 of the fluid reservoir ...

NANO-PCR: Methods and devices for nucleic acid amplification and detection

Nano-pcr: methods and devices for nucleic acid amplification and detection

Honeycomb tube

MICROFLUIDIC METERING OF FLUIDS

Microfluidic flow analyzer for pathological detection and method thereof

Honeycomb tube

Microfluidic flow analyzer for pathological detection and method thereof

Microfluidic flow analyzer for pathological detection and method thereof

MICROFLUIDIC METERING OF FLUIDS

Honeycomb tube

Nano-pcr: methods and devices for nucleic acid amplification and detection

Nano-pcr: methods and devices for nucleic acid amplification and detection

MICROFLUIDIC METERING OF FLUIDS

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

CAPILLARY BARRIER

ANALYSIS CARTRIDGE AND LIQUID PROMOTION CONTROLLER

IMMUNOASSAY PRODUCT AND PROCEDURE

MICRO-FLUID DEVICE WITH AT LEAST TWO CHANNELS A CONNECTING CONNECTION CHANNEL AND PROCEDURE FOR LEADING AND EXAMINING FLUIDS

PIPETTE SAMPLING SYSTEM

PROCEDURE AND SYSTEM FOR THE TREATMENT OF ELEMENTS IN THE MICRO YARDSTICK FOR THE RE-USE

MICRO FLUID NET TO FORMING THE DROPLET QUEUE AND PROCEDURE

MEDICINE PRODUCT FOR THE ANALYTENÜBERWACHUNG AND DRUG DELIVERY

MICRO-FLUID SPEICHERUND/ODER DOSING CONSTRUCTION UNIT

MANIPULATION OF MICRO PARTICLES IN MICRO-FLUID SYSTEMS

USE OF A MICRO-FLUID DEVICE FOR IDENTIFICATION, QUANTIFICATION AND AUTHENTIFIZIERUNG LATENT MARKER

HANDLING SET FOR THE ANALYSIS OF A LIQUID SAMPLE BY NUCLEIC ACID AMPLIFICATION

SUCKING IN AND DELIVERY OF SMALL LIQUID VOLUMES

DEVICE FOR THE MONITORING OF THE RIVER RATE

MICROANALYSIS MEASURING DEVICE AND MICROANALYSIS MEASURING PROCEDURE USING OF IT

MULTI-LAYER MICRO-FLUID DISTRIBUTION DEVICE

MICRO-FLUID DEVICE CONTAINING A CASE OF BLISTER

MICRO-FLUID ANALYSIS SYSTEMS AND - PROCEDURES WITH EXTREME HIGH THROUGHPUT

SYSTEMS AND PROCEDURES FOR SNAPPING THE CHANGE OF THE SOLUTION ENVIRONMENT OF SENSORS

Photometrische Messeinrichtung und photometrisches Messverfahren für eine Probenflüssigkeit

The invention relates to a test set (1) for a photometric measuring device, comprising a mixing container (2) which has a filling opening (3) and comprising a metering container (8) which can be sealingly inserted into the filling opening (3) of the mixing container (2) and which contains a liquid reagent (13) in a closed cavity (9). The cavity (9) has a closure plunger (11), which can be moved axially in the cavity (9), at a first end of the metering container (8), said closure plunger generating a specifiable filling pressure in the reagent (13), and the metering container (8) has a closure membrane (10) at a second metering container and which can be inserted into the mixing container (2). According to the invention, the closure membrane (10) is equipped with a predetermined breaking point (20) which breaks open when the filling pressure is exceeded in a defined manner as a result of an axial movement of the closure plunger (11), said predetermined breaking point (20) of the closure ...

SEQUENZIERUNG BY INCORPORATION

EINFACHE FÜR MASSENPRODUKTION GEEIGNETE BAUWEISE FÜR KOMPLEXE HYDROPNEUMATISCHE SYSTEME

The invention relates to a simple design for hydropneumatic systems, which comprise three-dimensional connections of heat exchangers, pumps, and measuring and control elements, wherein the three-dimensional system is divided into a system of two-dimensional superimposed elements where connections inside the levels are established by channels and connections between the levels are established by openings in the levels, and wherein said levels have additional two-dimensional components, comprising separating plates (T) made of material with good thermal conductivity, insulating plates (I) made of material with poor thermal conductivity, molding plates (F), regulating plates having movable parts, and special plates (S), which are connected into a block by being jointed tightly and areally on top of each other, wherein each molding plate (F) is disposed between separating plates (T) or insulating plates (I), cut-out channels conducting media inside the plate level are provided in the molding ...

TEST CONTAINER, OBJEKTTRÄGERREAKTIONSUND RETAINING FILE AS WELL AS OPERATING PROCEDURE

LIQUID TREATMENT IN MICRO-PRODUCED ANALYTIC DEVICES

PROCEDURE AND DEVICE FOR THE ASSORTMENT OF MICROSCOPIC PARTICLES.

ANALYSIS ON MEASUREMENT OF THE FLOW RESISTANCE

DEVICE FOR CHEMICAL MIXING AND REACTION

DEVICE AND PROCEDURE FOR THE ANALYSIS OF LIQUID SAMPLES

PROCEDURE FOR THE SEPARATION FROM SUSPENSIONS AND DEVICE

NIKROMASSSTABGETREUE DEVICE FOR CHEMICAL ANALYSIS

CARTRIDGE TO WOULD DRIVE THROUGH A CHEMICAL REACTION

THERMAL MICRO VALVES

BIOMOLECULAR KINETICS MEASURING PROCEDURE BY A FLOW MICRO ARRAY

DEVICE FOR THE HANDLING OF BALLS

MICRO-PRODUCED DETECTORS

DEVICE FOR THE REGULATION OF ANALYTEN IN SOLUTIONS

POLYNUKLEOTIDE AMPLIFICATION ANALYSIS WITH A MICRO-PRODUCED DEVICE

MICRO-FLUID SYSTEM AND PROCEDURE FOR ITS ENTERPRISE

PROCEDURE FOR THE MASS SPECTROMETRY

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

Portable sample analyzer cartridge

A sample analyzer cartridge for use at a point of care of a patient such as in a doctor's office, in the home, or elsewhere in the field. By providing a removable and/or disposable cartridge with all of the needed reagents and/or fluids, the sample analyzer can be reliably used outside of the laboratory environment, with little or no specialized training.

Multilayer Hydrodynamic Sheath Flow Structure

A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.

System and method for automated generation and handling of liquid mixtures

The invention relates to a system ( 1 ) for supplying a microfluidic subsystem with liquids, comprising a first valve ( 14, 29, 46 ) and a first fluidic duct ( 10, 25, 28 ), for connecting said first valve ( 14, 29, 46 ) with said microfluidic subsystem and supplying a first liquid, and a second fluidic duct ( 11 ), for connecting with said microfluidic subsystem and supplying a second liquid characterized in that said first valve ( 14, 29, 46 ) is suitable for closing with time resolution not worse than 100 msec, and parameters of said first fluidic duct ( 10, 15, 28 ) are chosen such that the value of X 1 [Pa −1 ], defined as: X 1 [Pa −1 ]=(0.5×10 −9+1/ E 1 )(α R1 L 1 2 /A 1 ) is lower than 10 4 Pa −1 , where E 1 is the Young modulus of the material, of which said first fluidic duct ( 10, 25, 28 ) is made, L 1 is the length of the said first fluidic duct ( 10, 25, 28 ), A 1 is the surface area of the lumen of the said first fluidic duct ( 10, 25, 28 ) and α R1 is a constant characterizing the geometry of the said first fluidic duct ( 10, 25, 28 ) in an equation for the hydraulic resistance R 1 of the said first fluidic duct: R 1 =α R1 ( L 1 μ/A 1 2 ) with μ denoting the dynamic viscosity coefficient of the fluid filling the said first fluidic duct ( 10, 25, 28 ) in the measurement of R 1 . The invention relates also to a method for producing microdroplets on demand in such a system.

Thermal Microvalves

The movement and mixing of microdroplets through microchannels is described employing silicon-based microscale devices, comprising microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. The discrete droplets are differentially heated and propelled through etched channels. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry to be incorporated in the same device.

Integrated Microfluidic Device and Methods

A microfluidic device for analyzing a sample of interest is provided. The microfluidic device can comprise a microfluidic device body, wherein the microfluidic device body comprises a sample preparation area, a nucleic acid amplification area, a nucleic acid analysis area, and a network of fluid channels. Each of the sample preparation area, the nucleic acid amplification area and the nucleic acid analysis area are fluidly interconnected to at least one of the other two areas by at least one of the fluid channels. Using the microfluidic device, sample preparation can be combined with amplification of a biologically active molecule, and a suitable biological sample can be provided for analysis and/or detection of a molecule of interest. The small-scale apparatus and methods provided are easier, faster, less expensive, and equally efficacious compared to larger scale equipment for the preparation and analysis of a biological sample.

Test device, reaction apparatus and reactive test method

A test device having a micro flow channel including a reaction part where a reactant that is reactive to a tested chemical dispersed in a tested fluid is fixed, and at least one actuator for actuating the tested fluid to move in at least one of two opposite sides of the micro flow channel so as to homogenize a density distribution of the tested chemical in the tested fluid. The tested fluid is sent in the micro flow channel a plurality of times.

Devices and method for enrichment and alteration of cells and other particles

The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.

Three-dimensional (3d) hydrodynamic focusing using a microfluidic device

A microfluidic device comprises inlets for a sample flow and an out-of-plane focusing sheath flow, and a curved channel section configured to receive the sample flow and out-of-plane focusing sheath and to provide hydrodynamic focusing of the sample flow in an out-of-plane direction, the out-of-plane direction being normal to a plane including the curved channel. Examples of the invention also include improved flow cytometers.

Control of operation conditions within fluidic systems

The invention provides methods of controlling environmental conditions within a fluidic system, where such environmental conditions can affect the operation of the system in its desired function, and fluidic channels, devices, and systems that are used in practicing these methods. Such methods are generally directed to environmental control fluids, the movement of such fluids through these systems, and the interaction of these fluids with other components of the system, e.g., other fluids or solid components of the system.

Apparatus for detecting target molecules and related methods

An apparatus for analysis of a sample and in particular of a biological sample. The apparatus contains a microfluidic chip with dies, adapted to be selectively activated or deactivated by presence of target molecules in the biological sample. The apparatus further contains a light source to emit light for illumination of the microfluidic chip and an optical filter to allow passage of the light from the dies once activated or deactivated by the presence of the target molecules. A method for pressurizing a microfluidic chip is also disclosed, where a chamber is provided, the chamber is connected with the microfluidic chip and pressure is applied to the chamber.

Device and apparatus

A device ( 1 ) for carrying out a chemical or biochemical reaction and detecting the results, such as an assay to detect a target nucleic acid in a sample, said device comprising (i) a first well ( 8 ) in which a chemical or biochemical reaction such as a nucleic acid amplification reaction may be effected in a liquid phase or a receiving means for such a first well; (ii) a first channel ( 16 ) extending from the first well; (iii) a lateral flow assay device ( 32 ) arranged to receive liquid contents from said first channel, optionally by way of second well, on a bibulous membrane thereon, wherein said membrane contains elements that are able to detect the products of the chemical or biochemical reaction such as a target nucleic acid. Methods for using such devices and apparatus for carrying out assays using these are also described and claimed.

Microfluidic system and method for automated processing of particles from biological fluid

A microfluidic system for automatically depleting particles not of interest from a biological sample, comprising: a sampling module configured to receive the sample; and one or more microfluidic protein and nucleic acid depletion modules fluidically coupled to the sampling module and comprising binding agents configured to selectively bind to abundant plasma proteins or nucleic acids. A method for automatically depleting particles not of interest from a sample, comprising: receiving the sample; subjecting the sample to a force that separates at least a portion of the particles not of interest from the sample, thereby isolating at least a portion of the target component; passing the isolated target copmonent into a chamber; circulating the isolated target component in the chamber; and selectively capturing proteins or nucleic acids with binding agents within the chamber.

Sanitary swab collection system, microfluidic assay device, and methods for diagnostic assays

Biohazard specimen collection containers are provided with an external disposable skin, that is stripped away and discarded after the biohazardous specimen is collected, thus reducing or eliminating objectionable or dangerous residues on the outside surfaces of the container. Further, we teach that the sample collection container with external disposable skin may also serve as an integrated microfluidic biosample processing and analytical device, thereby providing a single entry, disposable assay unit, kit and system for “world-to-result” clinical diagnostic testing. These integrated assay devices are provided with synergic, multiple safe-handling features for protecting healthcare workers who handle them. The modified collection containers and analytical devices find application, for example, in PCR detection of infectious organisms or pathogenic markers collected on a swab.

Cartridge for conducting a chemical reaction

A cartridge for conducting a chemical reaction includes a body having at least one flow path formed therein. The cartridge also includes a reaction vessel extending from the body for holding a reaction mixture for chemical reaction and optical detection. The vessel comprises a rigid frame defining the side walls of a reaction chamber. The frame includes at least one channel connecting the flow path to the chamber. The vessel also includes flexible films or sheets attached to opposite sides of the rigid frame to form opposing major walls of the chamber. In addition, at least two of the side walls are optically transmissive and angularly offset from each to permit real-time optical detection of analyte in the reaction chamber.

Sheath flow devices and methods

The invention relates generally to fluid processing and, in particular aspects, processing fluids for detection, selection, trapping and/or sorting of particulate moieties. Sheath flow devices described allow isolation of target species from fluid samples while avoiding non-specific binding of unwanted species to the surfaces of the separation device. Biological fluid processing, detection, sorting or selection of cells, proteins, and nucleic acids is described. The invention finds particular use in diagnostic settings, analyzing a patient's medical condition, monitoring and/or adjusting a therapeutic regimen and producing cell based products.

Particle Analysis in an Acoustic Cytometer

The present invention is a method and apparatus for acoustically manipulating one or more particles.

System for Treating Liquids

A system for treating liquids comprising: a fluidics unit having at least one functional unit; at least one liquid storage unit of the first type; at least one liquid storage unit of second type; a first liquid line; and a second liquid line, wherein a flow of liquid through the first liquid line directed from the fluidics unit to the liquid storage unit of first type is blocked, at least at times, by means of a first valve and a flow of liquid through the second liquid line in the direction from the liquid storage unit of second type to the fluidics unit is blocked, at least at times, by means of a second valve.

Method and system for transferring and/or concentrating a sample

A method of controlling the concentration of a sample flowing through a microchannel is provided. The method includes flowing a sample carrier fluid from a sample carrier channel and into an upstream portion of a microchannel such that the sample carrier fluid flows from the upstream portion and toward a downstream portion of the microchannel, and flowing a sheathing fluid from a sheathing fluid channel and into the upstream portion of the microchannel such that the sheathing fluid flows from the upstream portion and toward the downstream portion of the microchannel, and generally parallel to the sample carrier fluid. The method further includes detecting a concentration of a sample in the sample carrier fluid in the downstream portion of the microchannel, and adjusting a flow rate of at least one of the sample carrier fluid and the sheathing fluid based upon the detected concentration of the sample.

Mechanically Integrated and Closely Coupled Print Head and Mist Source

A deposition apparatus comprising one or more atomizers structurally integrated with a deposition head. The entire head may be replaceable, and prefilled with material. The deposition head may comprise multiple nozzles. Also an apparatus for three dimensional materials deposition comprising a tiltable deposition head attached to a non-tiltable atomizer. Also methods and apparatuses for depositing different materials either simultaneously or sequentially.

Method for performing a high throughput assay

A device and method is provided for performing a high throughput assay. The device includes a plate structure having a plate and a plurality of microfluidic structures positioned thereon. Each microfluidic structure defines a channel having an input and an output. At least one of the input and the output of the channel of each of the plurality of mircofluidic structures includes a first plurality of ports. In operation, the channels are filled with fluid and pressure gradients are generated between the fluids at the inputs and the fluids at the outputs of the channels. As a result, fluid flows through the channels toward the outputs.

Isolation and cultivation of stem/progenitor cells from the amniotic membrane of umbilical cord and uses of cells differentiated therefrom

The present invention relates to the generation of a mucin-producing cell using stem/progenitor cells obtained from the amniotic membrane of umbilical cord and therapeutic uses of such mucin-producing cells.

Apparatus for and method of processing biological samples

The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.

Moving microdroplets in a microfluidic device

The present invention relates to a system and method for moving samples, such as fluid, within a microfluidic system using a plurality of gas actuators for applying pressure at different locations within the microfluidic. The system includes a substrate which forms a fluid network through which fluid flows, and a plurality of gas actuators integral with the substrate. One such gas actuator is coupled to the network at a first location for providing gas pressure to move a microfluidic sample within the network. Another gas actuator is coupled to the network at a second location for providing gas pressure to further move at least a portion of the microfluidic sample within the network. A valve is coupled to the microfluidic network so that, when the valve is closed, it substantially isolates the second gas actuator from the first gas actuator.

Flow Step Focusing

Flow step focusing isolates and concentrates a molecule of interest by flowing a liquid comprising a molecule of interest through a main channel having an inlet and an outlet with application of a first pressure at the inlet; applying a voltage along the channel during the flowing, wherein the voltage is configured to have a polarity such that it drives the molecule of interest in a direction opposite the flow of the liquid; controlling the first pressure and/or the voltage in a manner so as to trap and concentrate the molecule of interest in a region of the main channel; and removing the concentrated molecule of interest from the channel by recovering a portion of the liquid from a side channel diverging from the main channel, wherein the side channel is maintained at a pressure lower than the first pressure. Also disclosed is an apparatus for such.

Device for Forming Drops in a Microfluidic Circuit

The invention relates to a device ( 1 ) for forming droplets in a microfluidic circuit, the device comprising a chamber ( 3 ) containing a first fluid and defined by two opposite walls ( 10, 11 ) that diverge relative to each other in at least one given direction, and a microchannel ( 8 ) containing a second fluid and leading into a zone of said chamber ( 3 ) that is upstream relative to the given direction, the outlet of the microchannel ( 8 ) into the chamber ( 3 ) constituting an enlargement in the flow section for the second fluid, and the enlargement giving rise to droplets ( 14 ) of the second fluid forming within the first fluid.

Test instrument for measuring analyte in sample, and method for measuring analyte using same

Disclosed is a test instrument for measuring an analyte in a liquid sample by a noble metal colloid aggregation measurement method. The test instrument involves a reaction chamber in which at least the liquid sample is to be reacted with a reagent, wherein the reagent is adhered on at least a part of a surface constituting the reaction chamber in a dried state, and the reagent enables the measurement of the analyte by a noble metal colloid aggregation measurement method. The test instrument additionally involves a supply section for supplying the liquid sample and a flow path for delivering the liquid sample that has been supplied to the supply section to the reaction chamber, wherein the liquid sample that has been supplied to the supply section is delivered to the reaction chamber through the flow path to cause the liquid sample to be brought into contact with the reagent that has been adhered in a dried state, thereby producing a difference in pressure between the supply section and the reaction chamber for the purpose of dispersing the reagent in the liquid sample. When the test instrument is used, the measurement based on an absorbance at a visible region can be achieved, the analyte can be measured accurately within a short time, and the measurement suitable for a POCT field can be achieved.

Fluid controlling apparatus and method of controlling fluid by using the same

A fluid controlling apparatus including at least one sample chamber for holding a fluid containing target materials; a cleaning chamber for holding a cleaning solution; a first multi-port connected to the at least one sample chamber through a first channel and connected to the cleaning chamber through a second channel; a filter portion, connected to the first multi-port through a third channel, for filtering the target materials; and a first pump, connected to the filter portion, for applying a pressure; and a method of controlling a fluid using the fluid controlling apparatus, which comprises passing the fluid containing the target materials from the at least one sample chamber to the filter portion; and cleaning a path of the fluid by passing the cleaning solution through the path.

Microfluidic chip

A microfluidic chip includes a base layer, a fluid layer, and a gas regulating layer. The base layer includes a microarray detecting zone. The microarray detecting zone includes a substrate, a photoresist pattern layer, a blocking layer, a bonding layer, at least one linker molecule, and a probe molecule. The bonding layer is covalently attached to the photoresist pattern layer. The at least one linker molecule is covalently bonded to the binding layer. The probe molecule is covalently bonded to the at least one linker molecule for specifically reacting with an under-test molecule. The fluid layer is disposed over the base layer, and includes plural flow channels for introducing or collecting detecting reagents. The gas regulating layer is disposed over the fluid layer for controlling open/close statuses of the flow channels, thereby controlling a flowing condition of a fluid in the fluid layer.

Apparatus and method for filter cleaning by ultrasound backwashing and filter movement during the filtration of biological samples

The present application is directed to the separation of a solid fraction from a fluid sample particularly a therapeutic cellular fraction from a biological sample such as a bone marrow sample by a porous filter which separates a filtration unit into an upper pre-filtration chamber into which a fluid sample requiring cell separation is introduced and a lower post-filtration chamber into which a fluid capable of transmitting an acoustic standing wave is introduced. An acoustic element is coupled to a substrate which is located within and at the bottom of the lower chamber and which resonates in response to the acoustic generating element and generates a standing wave through the two fluid phases and the filter to agitate the sample. Simultaneously, a cyclic process of vacuum draw, causes movement of the sample downwards through the filter. Vacuum pressure, fluid flow rate and frequency of vibration are controlled from a remote unit housing appropriate pumps and valves.

Methods of and devices for capturing circulating tumor cells

A device and methods are provided for efficient and quick capture of target cells through a main microchannel having capture elements immobilized thereon and manipulating a velocity profile of a sample as it passes through the main microchannel. The cell capture device may have a main microchannel with a depth slightly larger than the diameter of the target cells and a plurality of side microchannels. The side microchannels may have a depth smaller than the diameter of the target cells. The device and methods may be used for early cancer detection.

Microfluidic device with deformable valve

A microfluidic device includes a first microchannel, a second microchannel, and a valve comprising at least an input port and an output port, the ports respectively connected to the first microchannel and the second microchannel, the valve designed to control a flow of a liquid along a flow direction (z) defined by the ports; wherein the valve further comprises one or more walls joining the ports and defining a hollow chamber that is wider than each of the microchannels in a direction perpendicular to the flow direction, the walls at least partly deformable along a deformation direction (−y) intersecting the flow direction, such that the walls can be given at least a first deformation state and a second deformation state, such that the liquid can be pulled along the flow direction substantially more in the second deformation state than in the first deformation state.

Method and device for isolating cells from heterogeneous solution using microfluidic trapping vortices

A method of isolating cells includes providing a microfluidic device having at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel comprises at least one expansion region disposed along the length thereof. The at least one expansion region is an abrupt increase in a cross-sectional dimension of the at least one microfluidic channel configured to generate a vortex within the at least one expansion region in response to fluid flow. A solution containing a population of cells at least some of which have diameters ≧10 μm flows into the inlet. A portion of cells is trapped within vortex created within the at least one expansion region. The trapped cells may then released from the expansion region.

Apparatus and methods for transferring materials between locations possessing different cross-sectional areas with minimal band spreading and dispersion due to unequal path-lengths

Non-limiting exemplary embodiment(s) of apparatus(es) and method(s) are described for the conveyance of fluid media and entrained materials between two or more locations, each possessing a different cross-sectional area. Equidistant pathways incorporated into uniquely designed conduits enable this transference to occur with minimal band spreading and separation resolution loss due to undesirable flow patterns that arise from end effects. The design enables the conduits to be employed with locations of almost any description including process channels, surfaces, or even open volume of any size and used for almost any purpose. The conduits and/or associated locations may be empty of any contents or filled with gelatinous, porous, granular, or particulated material. The design of non-limiting exemplary embodiment(s) of apparatus(es) and method(s) may be easily adapted or configured as necessary.

Microfluidic devices

The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

Capillary dispenser

A capillary dispenser includes at least one plunger-cylinder unit having a cylinder module and a plunger module disposed together on an axis. The cylinder module has an inner cylinder that is pneumatically connected to at least one capillary. The plunger module includes a hollow plunger that is closable on one side and is movable in the inner cylinder along the axis between an upper end position and a lower end position of the plunger module.

Microfluidic devices for the capture of biological sample components

Methods and systems for selectively capturing analytes, such as cells, e.g., circulating tumor cells (CTCs), from fluid samples are disclosed. The methods include contacting the sample with an analyte binding moiety that selectively binds to the analytes; optionally separating first components of the sample including a majority of the analytes bound to the binding moieties from second components of the sample using size-based separation, e.g., in a microfluidic channel; adding to the first components of the sample a plurality of binding agents under conditions that enable a plurality of the binding agents to be linked to the analyte binding moieties to form multivalent tagging agents bound to the analyte; passing the first components of the sample past a surface to which is attached a plurality of capture agents that selectively bind to the binding agents; and capturing the analytes by providing conditions that enable the multivalent tagging agents bound to the analytes to bind to one or more of the capture agents.

Microfluidic cartridge for processing and detecting nucleic acids

A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.

Acoustic waves in microfluidics

Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one set of embodiments, droplets may be sorted using surface acoustic waves. The droplets may contain cells or other species. In some cases, the surface acoustic waves may be created using a surface acoustic wave generator such as an interdigitated transducer, and/or a material such as a piezoelectric substrate. The piezoelectric substrate may be isolated from the microfluidic substrate except at or proximate the location where the droplets are sorted, e.g., into first or second microfluidic channels. At such locations, the microfluidic substrate may be coupled to the piezoelectric substrate (or other material) by one or more coupling regions. In some cases, relatively high sorting rates may be achieved, e.g., at rates of at least about 1,000 Hz, at least about 10,000 Hz, or at least about 100,000 Hz, and in some embodiments, with high cell viability after sorting.

Method and device for high-throughput solution exchange for cell and particle suspension

A method of exchanging fluids with suspended particles includes providing a microfluidic device with a first inlet channel operatively coupled to a source of particles and a second inlet channel operatively coupled to an exchange fluid. A transfer channel is connected at a proximal end to the first inlet channel and the second inlet channel. First and second outlet channels are connected to a distal end of the transfer channel. The source of particles is flowed at a first flow rate into the first inlet channel while the exchange fluid is flowed at a second flow rate into the second inlet channel wherein the ratio of the second flow rate to the first flow rate is at least 1.5. Particles are collected in one of the first and second outlet channels while fluid substantially free of particles is collected in the other of the first and second outlet channels.

Test chip and test chip unit incorporated with test chip

The present invention is characterized in being provided with a chip main body, which is provided with a flow channel having the end portion thereof opened in the surface, and a sheet-like sealing member, which brings the inside of the flow channel into a hermetically closed state by covering at least the opening in the surface of the chip main body. The present invention is also characterized in that in the sealing member, a plurality of sheets are laminated, said sheets including a first sheet, which has ductility and elasticity such that the first sheet can be penetrated by means of a nozzle member, and a second sheet having ductility lower than that of the first sheet, the sheets adjacent to each other are bonded with an adhesive agent or a cohesive agent, and that the second sheet is positioned further toward the chip main body side than the first sheet.

Fluid sensing and control in a fluidic analyzer

Instrument-cartridge interfaces for fluidic analyzers that have an instrument and a removable cartridge are disclosed. For example, and in one illustrative embodiment, the instrument may include a needle that is adapted to penetrate a septum on a removable cartridge. In another illustrative embodiment, the instrument may include a plunger that is adapted to deform a deformable membrane on a removable cartridge. In yet another illustrative embodiment, the instrument may include a nozzle that is adapted to mate and seal with a flow channel on a removable cartridge. Techniques for detecting the flow rate in a flow channel on a removable cartridge, as well as the position of fluid in a flow channel of a removable cartridge, are also disclosed.

Microfluidic device for high-throughput cellular gradient and dose response studies

The ability to form and maintain gradients is essential for the study of response of cells to various stimuli. The invention includes devices and methods for the high-throughput, reproducible formation of gradients for the study of living cells. The invention includes microfluidics device with a lest chamber having a depth flanked by flow-through channels having a deeper depth. Flow of two different fluids through the flow-through channels results in the creation of a gradient by diffusion across the test chamber having essentially no flow.

Cassette For Sample Preparation

Apparatuses for preparing a sample are disclosed herein. The apparatuses include a chamber, a first valve at least partially disposed in the first chamber, a second valve at least partially disposed in the first chamber, and a pump comprising an actuator and nozzle.

Device for filtering blood

A device is proposed having a separating device for filtering a liquid sample and having a conveying device. A positive pressure or negative pressure can be generated by means of the conveying device, for accelerating or assisting the filtration.

Integrated Cartridge Housings For Sample Analysis

The invention relates to a cartridge housing for forming a cartridge capable of measuring an analyte or property of a liquid sample. The housing including a top portion having a first substantially rigid zone and a substantially flexible zone, a bottom portion separate from the top portion including a second substantially rigid zone, and at least one sensor recess containing a sensor. The top portion and the bottom portion are bonded to form the cartridge having a conduit over at least a portion of the sensor. The invention also relates to methods for forming such cartridges and to various features of such cartridges.

Micro-devices for disease detection

Among others, the present invention provides micro-devices for detecting or treating a disease, each comprising a first micro sensor for detecting a property of the biological sample at the microscopic level, and an interior wall defining a channel, wherein the micro sensor is located in the interior wall of the micro-device and detects the property of the biological sample in the microscopic level, and the biological sample is transported within the channel.

Apparatus for detecting tumor cells

Among others, the present invention provides apparatus for detecting circulating tumor cells, comprising a system delivery biological subject and a probing and detecting device, wherein the probing and detecting device includes a first micro-device and a first substrate supporting the first micro-device, the first micro-device contacts a biologic material to be detected and is capable of measuring at the microscopic level an electrical, magnetic, electromagnetic, thermal, optical, acoustical, biological, chemical, electro-mechanical, electro-chemical, electro-optical, electro-thermal, electro-chemical-mechanical, bio-chemical, bio-mechanical, bio-optical, bio-thermal, bio-physical, bio-electro-mechanical, bio-electro-chemical, bio-electro-optical, bio-electro-thermal, bio-mechanical-optical, bio-mechanical thermal, bio-thermal-optical, bio-electro-chemical-optical, bio-electro-mechanical-optical, bio-electro-thermal-optical, bio-electro-chemical-mechanical, physical or mechanical property, or a combination thereof, of the biologic subject.

The Use of Microfluidic Systems in the Electrochemical Detection of Target Analytes

The invention relates generally to methods and apparatus for conducting analyses, particularly microfluidic devices for the detection of target analytes.

Valved, Microwell Cell-Culture Device And Method

A valved microfluidics device, microfluidics cell-culture device and system incorporating the devices are disclosed. The valved microfluidics device includes a substrate, a microchannel through which liquid can be moved from one station to another within the device, and a pneumatic microvalve adapted to be switched between open and closed states to control the flow of fluid through a microchannel. The microvalve is formed of three flexible membranes, one of which is responsive to pneumatic pressure applied to the valve and the other two of which deform to produce a more sealable channel cross-section. The cell culture device provides valving to allow controlled loading of cells into the individual well of the device, and exchange of cell-culture components in the wells.

Flow control method and apparatuses

Aspects of the present disclosure are directed to the flow of analytes, particles or other materials. As consistent with one or more embodiments described herein, an apparatus includes a membrane having one or more pores in a membrane. First and second electrodes facilitate electrophoretic flow of analytes through the pore, and a third electrode controls movement of the particles in the pore by modulating the shape of an electric double layer adjacent sidewalls of pore. This modulation controls the strength of an electroosmotic field that opposes the electrophoretic flow of the analytes via the pore

Method and Apparatus for Generating Thermal Melting Curves in a Microfluidic Device

The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

MICROFLUIDIC VALVE AND METHOD OF MAKING SAME

The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve. 118.-. (canceled)19. A method of making a microfluidic valve , the method comprising:directing a dispensing head over an inlet hole in a microfluidic substrate;propelling a quantity of thermally responsive substance from the dispensing head into the inlet hole;maintaining a temperature of the microfluidic substrate so that the thermally responsive substance flows by capillary action into a microfluidic channel in communication with the inlet hole; andcovering the inlet hole.20. The method of claim 19 , wherein covering the inlet hole comprises covering two or more inlet holes.21. The method of claim 19 , wherein covering the inlet hole comprises covering two or more inlet holes for valves that are in a sample lane of the microfluidic substrate.22. The method of claim 19 , wherein covering the inlet hole comprises covering two or more inlet holes for valves that are in different sample lanes of the microfluidic substrate.23. The method of claim 19 , wherein covering the inlet hole comprises covering the microfluidic substrate with a layer of plastic.24. The method of claim 19 , wherein covering the inlet hole comprises covering the microfluidic substrate with a layer comprising a pressure sensitive adhesive.25. The method of claim 19 , wherein propelling a quantity of thermally responsive substance comprises propelling a quantity of thermally responsive substance to the bottom of the inlet hole.26. The method of claim 19 , wherein propelling a quantity of thermally responsive substance comprises ...

Device for analysis of cellular motility

A mesoscale fluidic system comprises a substrate having a sample chamber and an analysis chamber. The sample chamber comprises a cell permeable filter defining a sample application compartment and a conditioning medium compartment. The sample chamber has a sample inlet port in the sample application compartment. The analysis chamber has an entry port and an exit port. The conditioning medium compartment is in fluid communication with the entry port of the analysis chamber via a channel. The sample application compartment is below the cell permeable filter and the conditioning medium compartment is above the cell permeable filter. The mesoscale fluidic system is suited for analysing cellular motility in a sample. Also disclosed is a method of estimating the quantity of motile cells in a sample and a method of extracting motile cells from non-motile cells.

Particle separation

An example system includes an input channel having a first end and a second end to receive particles through the first end, a separation chamber, at least two output channels, and an integrated pump to facilitate flow through the separation chamber. The separation chamber is in fluid communication with the second end of the input channel. The separation chamber has a passive separation structure, the passive separation structure including an array of columns spaced apart to facilitate separation of particles in a flow based on a size of the particles. Each output channel is in fluid communication with the separation chamber to receive separated particles. The integrated pump is positioned within at least one of the input channel or one of the at least two output channels.

MICROFLUIDIC SIPHONING ARRAY FOR NUCLEIC ACID QUANTIFICATION

In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein. 120.-. (canceled)21. A device for processing a sample , comprising a chamber configured to (i) retain said sample during processing and (ii) permit gas flow from a location within said chamber through a wall of said chamber to a location external to said chamber.22. The device of claim 21 , further comprising a fluid flow path in fluid communication with said chamber claim 21 , wherein said fluid flow path is configured to provide a reagent to said chamber.23. The device of claim 21 , further comprising a siphon aperture in fluid communication with said chamber claim 21 , wherein said siphon aperture is configured to provide a reagent to said chamber.24. The device of claim 21 , wherein said wall comprises a polymeric material.25. The device of claim 24 , wherein said polymeric material is inelastic.26. The device of claim 24 , wherein said polymeric material is a thermoplastic.27. The device of claim 21 , wherein said wall is impermeable to water.28. The device of claim 21 , wherein said chamber comprises a plurality of walls claim 21 , which plurality of walls comprises said wall.29. The device of claim 21 , further comprising a pressure unit configured to supply a pressure drop across said wall claim 21 , which pressure drop is sufficient to induce said gas flow.30. A method for processing a sample claim 21 , comprising providing a device comprising a chamber that (i) retains said sample and (ii) permits gas flow from a location within said chamber through a wall of said chamber to a location ...

NON-INVASIVE MONITORING CANCER USING INTEGRATED MICROFLUIDIC PROFILING OF CIRCULATING MICROVESICLES

A microfluidic exosome profiling platform integrating exosome isolation and targeted proteomic analysis is disclosed. This platform is capable of quantitative exosomal biomarker profiling directly from 30 μL plasma samples within approximately 100 minutes with markedly enhanced sensitivity and specificity. Identification of distinct subpopulation of patient-derived exosomes is demonstrated by probing surface proteins and multiparameter analyses of intravesicular biomarkers in the selected subpopulation. The expression of IGF-1R and its phosphorylation level in non-small cell lung cancer (NSCLC) patient plasma is assessed, as a non-invasive alternative to the conventional biopsy and immunohistochemistry. The microfluidic chip, which may be fabricated of a glass substrate and a layer of poly(dimethylsiloxane), can include a first capture chamber, a second capture chamber, a serpentine microchannel, a first microchannel, a second microchannel, a sample inlet, a buffer inlet, a bead inlet, at least a first connector channel, and a reagent inlet. 1. A microfluidic chip comprising:a first capture chamber configured to enable immunomagnetic isolation;a second capture chamber configured to enable protein analysis;a serpentine microchannel connecting the first capture chamber and the second capture chamber;a first microchannel connecting the first capture chamber and the serpentine microchannel;a second microchannel connecting the serpentine microchannel and the second capture chamber;a sample inlet connected to the first capture chamber;a buffer inlet connected to the first capture chamber;a bead inlet;at least a first connector channel connected to the bead inlet and the first microchannel, wherein the first connector channel is connected to the first microchannel upstream of the serpentine microchannel and downstream of the first capture chamber; anda reagent inlet connected to the second microchannel upstream of the second capture chamber and downstream of the serpentine ...

MAGNETIC SEPARATION DEVICE AND METHOD OF USE

The current invention relates to the method and apparatus to magnetically separate biological entities with magnetic labels from a fluid sample. The claimed magnetic separation device removes biological entities with magnetic labels from its fluidic solution by using a soft-magnetic center pole with two soft-magnetic side poles. The claimed device further includes processes to dissociate entities conglomerate after magnetic separation. 1. A device for separating biological entities comprising:a magnetic field source having a first surface and a second surface;a soft magnetic center pole having a first end contacting said first surface, and a tip end tapering away from said first end, thereby concentrating magnetic flux from said first surface towards said tip end;a first soft magnetic side pole having a first base end and a second end;a second soft magnetic side pole having a second base end and a third end;a channel contacting said tip end contains a fluidic sample comprising said biological entities, thereby causing separation of said biological entities bound with magnetic labels towards said tip end;wherein said first and second base ends contact said second surface;wherein said second and third ends are located on opposing sides of said tip end;wherein said magnetic flux from said tip end divides into each of said second and third ends, thereby forming a flux closure within said center pole and said first and second side poles; andwherein said biological entities bound with magnetic labels are removed from said channel after separating said channel from said tip end.2. The device according to claim 1 , wherein said magnetic field source comprises a permanent magnet having a first polarization surface contacting a top surface of a soft magnetic shield claim 1 , and a second polarization surface opposing said first polarization surface claim 1 , wherein said first surface is said second polarization surface and said second surface is said top surface.3. The ...

Devices And Method For Enrichment And Alteration Of Cells And Other Particles

The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample. 1150-. (canceled)151. A method of enriching a sample in fetal cells relative to maternal cells , the method comprising:(a) introducing a maternal blood sample into a microfluidic device capable of enriching fetal nucleated cells relative to maternal cells based on size, shape, deformability, or affinity to produce an enriched sample;(b) lysing fetal nucleated cells in the enriched sample to release fetal nuclei; and(c) detecting fetal nuclei.152. The method of claim 151 , wherein step (b) comprises lysing all cells in the enriched sample and collecting fetal nuclei.153. The method of claim 151 , wherein step (b) comprises selectively lysing fetal nucleated cells relative to maternal cells.154. The method of claim 151 , wherein the microfluidic device comprises a channel having a structure that deterministically directs fetal nucleated cells in a first direction and at least some maternal cells in a second direction based on deterministic lateral displacement.155. The method of claim 154 , wherein the microfluidic device is a duplex device comprising a channel comprising a first section comprising first and second outer regions claim 154 , each outer region comprising a structure that deterministically directs particles ...

SYSTEM AND METHOD FOR GENERATING OR ANALYZING A BIOLOGICAL SAMPLE

A system including a reaction valve having a mating side and a valve inlet. The reaction valve includes a sample chamber, and the valve inlet is in fluid communication with the sample chamber. The system also has a manifold having an engagement surface. The manifold may include first and second manifold ports at the engagement surface. The engagement surface and the mating side of the reaction valve may be positioned adjacent to each other along an interface. The system may also include a positioning assembly that is operatively coupled to at least one of the reaction valve or the manifold. The positioning assembly is configured to move at least one of the reaction valve or the manifold along the interface to fluidly disconnect the valve inlet from the first manifold port and to fluidly connect the valve inlet to the second manifold port. 133-. (canceled)34. A system comprising:a reaction valve including a sample chamber;a positioning assembly operatively coupled to the reaction valve and configured to rotate the reaction valve about an axis of rotation; andan imaging assembly positioned proximate to the sample chamber of the reaction valve and configured to obtain at least one image of the sample chamber, the positioning assembly configured to rotate the reaction valve to move the sample chamber relative to the imaging assembly, the positioning assembly configured to at least one of (a) rotate the reaction valve after the imaging assembly has imaged the sample chamber or (b) rotate the reaction valve as the imaging assembly images the sample chamber.35. The system of claim 34 , wherein the imaging assembly has an imaging plane claim 34 , the sample chamber moving along the imaging plane as the reaction valve is rotated.36. The system of claim 34 , wherein the at least one image includes first claim 34 , second claim 34 , and third images and the imaging assembly is configured to sequentially obtain the first claim 34 , second claim 34 , and third images claim 34 , ...

MANIPULATING DROPLET SIZE

The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid. 117-. (canceled)18. A system for forming droplets , the system comprising:a microfluidic substrate comprising a sample channel and a carrier fluid channel that connects to the sample channel at a junction;a manifold in fluid communication with the microfluidic substrate, the manifold configured to deliver an aqueous fluid to the sample channel and an immiscible carrier fluid to the carrier channel to thereby form droplets of aqueous fluid surrounded by carrier fluid at the junction; anda pump in fluid communication with the microfluidic substrate, the pump configured to regulate pressure of the aqueous fluid at the junction.19. The system of claim 18 , further comprising a fluid interface comprising a plurality of interconnects each aligned with and forming a seal with an inlet of one of the plurality of microfluidic channels.20. The system of claim 19 , wherein the manifold is in fluid communication with the microfluidic substrate via the fluid interface.21. The system of claim 18 , wherein the microfluidic substrate comprises a top plate and a bottom plate.22. The system of claim 18 , wherein the channels are etched into one or both of the top plate and the bottom plate.23. The system of claim 18 , further comprising an outlet channel downstream of the junction.24. The system of claim 18 , wherein the pump is coupled to an electronic pressure regulator.25. The system of claim 18 , wherein the pump is an external compressor comprising a reservoir of nitrogen claim 18 , argon claim 18 , or air.26. The system of claim 18 , wherein the pump is an internal air cylinder with a linear actuator. ...

DROPLET FLUID CONNECTIONS

Drop-to-drop connection schemes are described for putting a microfluidic device in fluidic communication with a fluid source or another microfluidic device, including but not limited to, putting a microfluidic device in fluidic communication with the perfusion manifold assembly. 1. A first fluidic device comprising a substrate having a first mating surface , said first mating surface comprising one or more fluidic ports , wherein said first mating surface is adapted to stably retain a first protruding liquid droplets comprising a first liquid at the one or more fluidic ports , wherein said port of said first fluidic device comprising said first protruding liquid droplet is aligned with a port of a second fluidic device comprising a second protruding droplet so as to permit fluidic communication.2. The fluidic device of claim 1 , wherein said first mating surface comprises one or more regions surrounding the one or more fluidic ports claim 1 , and wherein said regions are adapted to resist wetting by said first liquid.3. The fluidic device of claim 2 , wherein said regions are adapted to be hydrophobic.4. The fluidic device of claim 2 , wherein said one or more regions comprise a first material selected to resist wetting by said first liquid.5. The fluidic device of claim 4 , wherein the first material is selected from the group consisting of poly-tetrafluoroethylene (PTFE) claim 4 , a perfluoroalkoxy alkane (PFA) claim 4 , fluorinated ethylenepropylene (FEP) claim 4 , polydimethylsiloxane (PDMS) claim 4 , nylon claim 4 , polypropylene claim 4 , polystyrene and polyimide.6. The fluidic device of claim 4 , wherein the substrate comprises said first material.7. The fluidic device of claim 4 , wherein said first material is bonded claim 4 , adhered claim 4 , coated or sputtered onto said first surface.8. The fluidic device of claim 4 , wherein said first material comprises a hydrophobic gasket.9. The fluidic device of claim 2 , wherein the one or more regions are ...

SYSTEM AND METHOD FOR PROCESSING BIOLOGICAL SAMPLES

A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem including an actuation substrate, and a set of pins interacting with the actuation substrate, and a spring plate configured to bias at least one pin in a configurations, the valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; and a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols. 1. A system for processing a biological sample comprising:a cartridge platform receiving a cartridge including a fluidic pathway, wherein the cartridge platform comprises an access region configured to align with the fluidic pathway; a pin comprising a first end, a second end opposing the first end, and a pin slot defined along a length of the pin between the first and the second end, the pin actuatable along a displacement axis extending through the first end, the second end, and the access region; and', 'an actuation substrate translatable along an actuation axis between a first substrate position, wherein a first substrate feature of the actuation substrate passes through the pin slot to transmit the pin through the access region; and a second substrate position, wherein a second substrate feature of the actuation substrate passes through the pin slot to transmit the pin away from the access region., 'a valve actuation subsystem comprising2. The system of claim 1 , wherein the valve actuation system further comprises a spring coupled to a region of the pin claim 1 , ...

METHOD, SYSTEM, AND DEVICE FOR ANALYTE DETECTION AND MEASUREMENT USING LONGITUDINAL ASSAY

Embodiments of the invention provide methods, systems, and devices for detection and measurement of an analyte or analytes. In one embodiment, the invention provides an assay system comprising a cartridge device including: one or more reservoir portions for holding one or more liquids; and at least one assay portion for receiving the one or more liquids from the at least one reservoir portion, the at least one assay portion having a plurality of binding sites over which the one or more liquids from the one or more reservoirs can be flowed repeatedly (more than one time); and a measurement device for measuring binding of one or more analytes in the one or more liquids to the plurality of binding sites. 1. An assay system comprising: at least one reservoir portion for holding one or more liquids; and', 'at least one assay portion for receiving the one or more liquids from the at least one reservoir portion, the at least one assay portion having a plurality of binding sites over which the one or more liquids can be repeatedly flowed; and, 'a cartridge device includinga measurement device for measuring binding of one or more analytes in the one or more liquids to the plurality of binding sites.2. The assay system of claim 1 , further comprising:an interface into which the cartridge device is received and removed, wherein the interface includes an apparatus for controlling flow or movement of the one or more liquids from the at least one reservoir portion through the at least one assay portion,wherein the apparatus for controlling flow of the one or more liquids can independently control at least one of the following:the rate of flow of the at least one liquid across the at least one assay portion,the duration of flow of the at least one liquid across the at least one assay portion, orthe number of times a quantity of the at least one liquid is flowed over the at least one assay portion.3. The assay system of further comprising:a flow rate sensor,wherein the interface ...

HIGH/HYPERVELOCITY PARTICLE CAPTURE AND ANALYSIS METHOD AND APPARATUS

In various embodiments a capture surface for capturing high velocity and hypervelocity dust and ice particles is provided. In certain embodiments the capture surface is comprised of a soft metal that is chosen to optimize particle capture efficiency, to minimize thermal degradation of chemicals and biochemical in the particles, and to present the captured particles to an analyzer for chemical and biochemical analysis of the particles and their contents. In various embodiments capture chambers comprising one or more such capture surfaces are provided as well as methods of use thereof. 1. A particle capture surface configured for capture of high and/or hyper velocity dust , aerosol , and/or ice particles , wherein said capture surface is comprised of soft metal that maximizes particle capture efficiency , minimizes thermal degradation and shock degradation of chemical and biochemical components in the particles , and said surface is configured to present the captured particles , or components therein , on said surface for direct analysis or to deliver said particles , or component therein , to an analyzer for chemical and/or biochemical analysis of the particles and their component contents.2. The particle capture surface of claim 1 , wherein said surface is configured to deliver said particles to an analyzer for chemical and/or biochemical analysis of the particles and their contents.3. The particle capture surface according to any one of - claim 1 , wherein said surface is configured to capture extraterrestrial dust claim 1 , aerosol claim 1 , and/or ice particles.4. The particle capture surface according to any one of - claim 1 , wherein said surface is configured to capture extraterrestrial dust claim 1 , aerosol claim 1 , and/or ice particles in high earth orbit.5. The particle capture surface according to any one of - claim 1 , wherein said surface is configured to capture extraterrestrial dust claim 1 , aerosol claim 1 , and/or ice particles at high altitude.6. ...

FLUID HANDLING DEVICE AND METHOD OF USING THE SAME

A fluid handling device includes: a first channel through which liquid flows by capillarity; a liquid reservoir which communicates with an upstream end of the first channel and stores liquid; a liquid introduction part which communicates with the liquid reservoir and includes a taper part whose diameter decreases from an opening part toward the liquid reservoir; a stop valve disposed on a downstream end of the first channel and including a step part where a cross-sectional area of the channel in a direction orthogonal to a direction in which liquid flows discontinuously increases; and a second channel which communicates with a downstream end of the first channel, the second channel being a channel through which fluid flows. 1. A fluid handling device comprising:a first channel through which liquid flows by capillarity;a liquid reservoir which communicates with an upstream end of the first channel and stores liquid;a liquid introduction part which communicates with the liquid reservoir and includes a taper part whose diameter decreases from an opening part toward the liquid reservoir;a stop valve disposed on a downstream end of the first channel and including a step part where a cross-sectional area of the channel in a direction orthogonal to a direction in which liquid flows discontinuously increases; anda second channel which communicates with a downstream end of the first channel, the second channel being a channel through which fluid flows.2. The fluid handling device according to claim 1 , wherein the downstream end of the first channel opens at an internal wall of the second channel.3. The fluid handling device according to claim 1 , wherein claim 1 , in the direction orthogonal to the direction in which liquid flows through a connecting part between the liquid reservoir and the liquid introduction part claim 1 , an area of an opening part of the connecting part is smaller than a cross-sectional area of the liquid reservoir.4. A method of using a fluid handling ...

Methods and Systems for Enhanced Microfluidic Processing

Methods and systems are provided for a microfluidic cartridge including a high performance actuator useful for analyte detection, labeling and analysis. Microfluidic processing systems are to carry out chemical or biochemical reactions, or sequences of reactions, with small volumes (typically between 1 microliter and 10 milliliters) of reactants and products. A microfluidic processing system can comprise a network of tubes interfaced with discrete components such as valves and sensors, or an integrated device made of plastic, glass, metal, or other materials, or a combination of materials, with components such as valves and sensors built into the device and connected by flow passageways formed in the material.

MICROFLUIDIC SIPHONING ARRAY FOR NUCLEIC ACID QUANTIFICATION

In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein. 1. A device for processing a biological sample , comprising:a plurality of chambers configured to retain said biological sample during processing; andat least one gas permeable film or membrane covering said plurality of chambers, wherein said gas permeable film or membrane is configured to permit gas flow from a chamber of said plurality of chambers to a location external to said chamber.2. The device of claim 1 , wherein said at least one gas permeable film or membrane is a plurality of gas permeable films or membranes claim 1 , and wherein said chamber is covered by a gas permeable film or membrane of said plurality of gas permeable films or membranes.3. The device of claim 1 , wherein said at least one gas permeable film or membrane seals said plurality of chambers.4. The device of claim 1 , further comprising a fluid flow path in fluid communication with said plurality of chambers claim 1 , wherein said fluid flow path is configured to provide reagents to said plurality of chambers.5. The device of claim 4 , further comprising a plurality of siphon apertures in fluid communication with said fluid flow path and said plurality of chambers claim 4 , wherein said plurality of siphon apertures is configured to provide reagents from said fluid flow path to said plurality of chambers.6. The device of claim 1 , wherein said at least one permeable film or membrane comprises a polymer.7. The device of claim 1 , wherein said at least one gas permeable film or membrane has a thickness of at most about ...

METHOD FOR PROTECTING AND UNPROTECTING THE FLUID PATH IN A CONTROLLED ENVIRONMENT ENCLOSURE

A controlled environment enclosure comprises a robotic arm manipulation system used to protect and unprotect a fluid path and a swab within the controlled environment enclosure. The apparatus allows the fluid path to be protected against dangerous decontamination vapors and chemicals before the controlled environment enclosure is decontaminated. The apparatus allows the fluid path to be unprotected without the use of gloves or other means that degrade the integrity of the controlled environment enclosure when decontamination is completed. The apparatus and method allow for the protecting, unprotecting and decontaminating sequences to be automated. In some embodiments the fluid path comprises a fill needle that can removably and aseptically be sealed with a disposable monolithic injection moulded polymeric fill needle sheath. The apparatus and method further allow for the use of a swab disposed in a swab holder that is aseptically and removably sealable to a swab cap to protect the swab against decontamination vapors. 1. A pharmaceutical fill needle system for dispensing pharmaceutical fluids within an aseptic chamber , the fill needle system comprising:a first sterilizable encapsulation portion comprising at least one first mechanical engagement feature and a first sealable surface;a fill needle aseptically sealed to the first encapsulation portion and having a fluid receiving end and a fluid dispensing end, the fluid dispensing end protruding from the first encapsulation portion;a sterilizable fluid path aseptically sealed to the first encapsulation portion, a proximal end of the fluid path being in fluid communication with the fluid receiving end of the fill needle; anda second sterilizable encapsulation portion comprising at least one second mechanical engagement feature and a second sealable surface, the at least one second mechanical engagement feature releasably engaged with the at least one first mechanical engagement feature and the second sealable surface ...

Fluid handling device

The objective of the present invention is to provide a fluid handling device capable of easily isolating droplets. This objective is achieved by means of a fluid handling device which includes a first flow passage through the interior of which, when a fluid containing a droplet is delivered, the droplet can move, a first chamber which captures the droplet moving through the first flow passage, a second chamber into which the droplet captured by the first chamber can move, and a second flow passage providing communication between the first chamber and the second chamber, wherein the second flow passage is capable of selectively allowing the droplet to pass, or restricting passage of the droplet.

INSTRUMENT FOR PREFORMING A DIAGNOSTIC TEST ON A FLUIDIC CARTRIDGE

Instrument for performing a diagnostic test on a fluidic cartridge A cartridge reader is for carrying out a diagnostic test on a sample contained in a fluidic cartridge inserted into the reader. The fluidic cartridge comprises a fluidic layer comprising at least one sample processing region, at least one collapsible blister containing a liquid reagent, a pneumatic interface, an electrical interface and at least one mechanical valve. The reader comprises a housing; an upper clamp occupying a fixed position relative to the reader, and a lower clamp, movable relative to the first clamp, wherein the upper clamp and the lower clamp define a cartridge receiving region therebetween. The reader comprises a thermal module comprised in the lower clamp, wherein the thermal module comprises at least one thermal stack for heating the at least one sample processing region of the cartridge inserted into the reader. The reader comprises at least one mechanical actuator for actuating the mechanical valve comprised in the cartridge inserted into the reader. 123-. (canceled)24. A fluidic cartridge for carrying out a diagnostic test on a sample contained therein , the cartridge comprising: a housing;', 'an upper clamp occupying a fixed position relative to the reader and a lower clamp movable relative to the upper clamp, wherein the upper clamp and the lower clamp define a cartridge receiving region therebetween, wherein the cartridge receiving region comprises a tray configured to move between an open position and a closed position and to receive a cartridge;', 'a thermal module comprised in the lower clamp;', 'a pneumatics module comprising a plurality of pneumatic interface;', 'at least one mechanical actuator;', 'at least one blister actuator;', 'an electrical module comprising a plurality of pins; and', 'a barcode reader, wherein at least part of each of the pneumatics module, the at least one mechanical actuator, the at least one blister actuator, the electrical module and the ...

FLUIDIC CHANNEL FOR A CARTRIDGE

A cartridge for collecting sample material may include a cartridge body and a fluid reservoir. The cartridge body may define a capless sample well port configured to receive the sample material and a fluidic channel in fluid communication with the capless sample well port. The fluidic channel may include a sample fluidic channel portion and may be configured such that an effect of gravity on the sample material within the sample fluidic channel portion does not overcome a capillary action of the fluidic channel. The fluidic channel may extend between the capless sample well port and the fluid reservoir. The fluidic channel may be configured to direct the sample material towards the fluid reservoir when a pressure is applied within the fluidic channel. 1. A cartridge comprising:a cartridge body defining a capless sample well port configured to receive a sample material and a fluidic channel in fluid communication with the capless sample well port, wherein the fluidic channel comprises a first fluidic channel portion extending along a first axis and a second fluidic channel portion extending along a second axis, wherein the first axis is at an angle to the second axis; anda fluid reservoir, wherein the fluidic channel extends between the capless sample well port and the fluid reservoir, wherein the fluidic channel is configured to direct the sample material towards the fluid reservoir when a pressure is applied within the fluidic channel.2. The cartridge of claim 1 , further comprising a fluid drive port in fluid communication with the fluidic channel claim 1 , wherein the fluid drive port is configured to be operably connected to a pressure source such that a positive pressure is applied within the fluidic channel to direct the sample material towards the fluid reservoir.3. The cartridge of claim 1 , wherein the first and second fluidic channel portions define a channel depth of less than or equal to 3 mm and a channel width of less than or equal to 3 mm.4. The ...

CAPLESS SAMPLE WELL PORT FOR A CARTRIDGE

A cartridge for collecting sample material may include a cartridge body, a filter, a fluid reservoir, and a fluid drive port. The cartridge body may define a capless sample well port configured to receive a sample material and a fluidic channel in fluid communication with the capless sample well port. The filter may be positioned between the capless sample well port and the fluidic channel. The fluidic channel may extend between the capless sample well port and the fluid reservoir. The fluid drive port may be in fluid communication with the fluidic channel. The fluid drive port may be configured to be operably connected to a pressure source such that a pressure is applied within the fluidic channel to direct the sample material towards the fluid reservoir. 1. A cartridge comprising:a cartridge body defining a capless sample well port configured to receive a sample material and a fluidic channel in fluid communication with the capless sample well port;a filter positioned between the capless sample well port and the fluidic channel;a fluid reservoir, wherein the fluidic channel extends between the capless sample well port and the fluid reservoir; anda fluid drive port in fluid communication with the fluidic channel, wherein the fluid drive port is configured to be operably connected to a pressure source such that a pressure is applied within the fluidic channel to direct the sample material towards the fluid reservoir.2. The cartridge of claim 1 , wherein the filter defines a bubble point of less than or equal to 4 claim 1 ,000 Pa.3. The cartridge of claim 1 , wherein the fluidic channel is configured to provide a capillary action on the sample material passing through the fluidic channel.4. The cartridge of claim 1 , wherein the capless sample well port is located between the fluid drive port and the fluid reservoir.5. The cartridge of claim 1 , wherein the fluidic channel further comprises an overflow fluidic channel portion claim 1 , wherein the overflow fluidic ...

MICROFLUIDIC DEVICE FOR ACCOMMODATING, ISOLATING, TREATING, AND/OR PROCESSING CELLS HAVING AN INLET CHAMBER WITH A CHUTE STRUCTURE

The present invention relates to a microfluidic device suitable for accommodating, isolating, treating and/or processing cells. The device comprises an inlet chamber in fluid communication with at least one feeding channel being arranged in an essentially horizontal direction. The inlet chamber is suitable for receiving a volume of a liquid sample comprising at least one cell, and has an opening at its upward facing end which has a circular, ellipsoidal or polygonal cross-section. Further, the microfluidic device has a chute structure which defines a flow path for guiding the cells from the inlet chamber into the at least one feeding channel. 2. The microfluidic device according to claim 1 , wherein the sloped surface has a convex curvature suitable for focusing and/or directing the flow path towards the inlet of the at least one feeding channel.3. The microfluidic device according to claim 1 , wherein the chute structure is arranged in the transition section between the inlet chamber and at least one feeding channel claim 1 , and wherein the chute structure further comprises a gradual or stepwise slope in the feeding channel's lower surface.4. The microfluidic device according to claim 1 , wherein the horizontal feeding channel has a circular claim 1 , ellipsoidal or polygonal cross section.5. The microfluidic device according to claim 1 , wherein the chute structure is arranged in the transition section between the inlet chamber and at least one feeding channel claim 1 , and wherein claim 1 , in the transition section claim 1 , the horizontal feeding channel has an initial height of between ≥50 μm and ≤200 μm claim 1 , which height gradually or stepwise tapers to between ≥8 μm and ≤50 μm.6. The microfluidic device according to claim 5 , wherein at least stretches of the inlet chamber and/or the feeding channel comprise an anti-adhesive coating.7. The microfluidic device according to claim 6 , wherein the diameter of the inlet chamber is between ≤4 mm claim 6 , ...

ENHANCED CELL/BEAD ENCAPSULATION METHODS AND APPARATUSES

A method of encapsulating a solid sample in a droplet, the method including flowing a continuous phase through a first fluid channel at a first flow rate; flowing a dispersed phase through a second fluid channel at a second flow rate, the dispersed phase including a plurality of particles, cells or beads; trapping the plurality of particles, cells or beads in a mixing region that receives the dispersed phase and the continuous phase; and reducing the first flow rate to encapsulate the trapped particles, cells or beads in droplets of the dispersed phase generated when the dispersed phase and the continuous phase exit the mixing region through an orifice. 1. A method of encapsulating a solid sample in a droplet , the method comprising:flowing a continuous phase through a first fluid channel at a first flow rate;flowing a dispersed phase through a second fluid channel at a second flow rate, the dispersed phase comprising a plurality of particles, cells or beads;trapping the plurality of particles, cells or beads in a mixing region that receives the dispersed phase and the continuous phase; andreducing the first flow rate to encapsulate the trapped plurality of particles, cells or beads in droplets of the dispersed phase generated when the dispersed phase and the continuous phase exit the mixing region through an orifice.2. The method according to claim 1 , wherein a pump and/or a pressure regulator is used to:adjust a flow rate and/or a pressure of the continuous phase or the dispersed phase to trap the plurality of particles cells or beads of the dispersed phase in the mixing region in a first mode such that the plurality of particles, cells or beads of the dispersed phase are prevented from flowing through the orifice; andadjust a flow rate and/or a pressure of the continuous phase or the dispersed phase to allow the plurality of particles, cells or beads to flow through the orifice such that the plurality of particles, cells or beads are encapsulated in droplets of ...

MANIPULATION OF FLUIDS, FLUID COMPONENTS AND REACTIONS IN MICROFLUIDIC SYSTEMS

Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply. 120-. (canceled)21. A method for partitioning a fluid sample , the method comprising:providing a microfluidic device having a substrate comprising a plurality of microwells in fluid communication with an inlet;introducing a first fluid into the inlet of the microfluidic device, the first fluid comprising a biological sample and a plurality of beads, each bead comprising a reactive component for binding a target molecule from the biological sample to the bead; a subvolume of the first fluid,', 'a portion of the biological sample, and', 'more than one bead; and, 'introducing a second fluid immiscible with the first fluid into the microfluidic device and flowing the second fluid towards each of the plurality of microwells so as to form partitions of fluid in corresponding microwells, wherein at least some of the partitions include'}maintaining each partition of fluid in corresponding microwells for detection of contents in each partition of fluid.22. The method of claim 21 , wherein a subset of the partitions do not include any beads and a second subset of the partitions each include a single bead.23. The method of claim 21 , wherein each partition of fluid is separated from the others by the second fluid.24. The method of claim 21 , wherein the second fluid is an oil.25. The method of claim 21 , further ...

Flow cell and liquid feed system

A flow cell ( 2 ) includes a plate-shaped body 21 that is almost rectangular in a plan view, an introduction portion ( 22 ) that is made of a concave portion formed in the upper surface of the body ( 21 ), a channel ( 23 ) that is formed inside the body ( 21 ) and has one end connected to the lower end of the introduction portion ( 22 ), and a delivery portion ( 24 ) that is made of a concave portion formed in the body ( 21 ) and has the lower end connected to the other end of the channel ( 23 ). The introduction portion ( 22 ) is formed to cause the meniscus of a liquid introduced from an opening to apply, to the liquid, a positive pressure or a negative pressure whose absolute value is smaller than that of a negative pressure applied to the liquid introduced into the channel ( 23 ) by the meniscus of the liquid. The channel ( 23 ) is formed to cause the meniscus of the liquid introduced into the channel ( 23 ) to apply a negative pressure to the liquid. This invention provides a flow cell and a liquid feed system capable of more easily controlling feed of a trace amount of liquid.

ORGANISM IDENTIFICATION PANEL

Methods and containers are provided for identifying a species, illustratively a bacterial species. Illustrative methods comprise amplifying various genes in the nucleic acid from the bacterial species in a single reaction mixture using pairs of outer first-stage primers designed to hybridize to generally conserved regions of the respective genes to generate a plurality of first-stage amplicons, dividing the reaction mixture into a plurality of second-stage reactions, each using a unique pair of second-stage primers, each pair of second-stage primers specific for a target bacterial species or subset of bacterial species, detecting which of the second-stage reactions amplified, and identifying the bacterial species based on second-stage amplification. Methods for determining antibiotic resistance are also provided, such methods also using first-stage primers for amplifying genes known to affect antibiotic resistance a plurality of the second-stage reactions wherein each pair of second-stage primers specific for a specific gene for conferring antibiotic resistance. 131-. (canceled)32. A method for identifying a bacterial species comprising the steps ofobtaining a sample containing the bacterial species,amplifying, in a single reaction mixture containing nucleic acid from the sample, a plurality of bacterial genes using pairs of outer first-stage primers designed to hybridize to generally conserved regions of the bacterial genes to generate a plurality of first-stage amplicons,dividing the reaction mixture into a plurality of second-stage reactions,subjecting each of a plurality of the second-stage reactions to amplification conditions, wherein each of the plurality of second-stage reactions uses a pair of second-stage primers, each pair of second-stage primers specific for a different target bacterial species or group of bacterial species,generating an amplification curve for each of the second-stage reactions that amplified,determining a crossing point for each ...

MICROFLUIDIC SYSTEM FOR AMPLIFYING AND DETECTING POLYNUCLEOTIDES IN PARALLEL

The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously. 1. A method of carrying out amplification on a plurality of samples , the method comprising:introducing each of the plurality of samples into an amplification zone of a multi-lane microfluidic cartridge;isolating each of the plurality of samples within the multi-lane microfluidic cartridge;independently thermally cycling each amplification zone; andamplifying polynucleotides contained within each of the plurality of samples.2. The method of claim 1 , further comprising detecting the presence of a polynucleotide or a polynucleotide probe in the plurality of samples.3. The method of claim 1 , wherein independently thermally cycling each amplification zone comprises heating each amplification zone with a plurality of heaters.4. The method of claim 1 , wherein independently thermally cycling each amplification zone comprises heating each amplification zone with four heaters.5. The method of claim 1 , wherein independently thermally cycling each amplification zone comprises heating each amplification zone with two long heaters and two short heaters.6. The method of claim 1 , wherein isolating each of the plurality of samples comprises closing at least one valve.7. A method of carrying out amplification on a plurality of samples claim 1 , the method comprising:introducing a sample of a plurality of samples into an amplification zone in a lane of a multi-lane microfluidic cartridge;isolating the sample from the plurality of samples within the multi-lane ...

KINETIC EXCLUSION AMPLIFICATION OF NUCLEIC ACID LIBRARIES

An example method includes reacting a first solution and a different, second solution on a flow cell by flowing the first solution over amplification sites on the flow cell and subsequently flowing the second solution over the amplification sites. The first solution includes target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates and replication enzymes. The target nucleic acids in the first solution transport to and bind to the amplification sites at a transport rate. The first reagent mixture amplifies the target nucleic acids that are bound to the amplification sites to produce clonal populations of amplicons originating from corresponding target nucleic acids. The amplicons are produced at an amplification rate that exceeds the transport rate. The second solution includes a second reagent mixture and lacks the target nucleic acids. The second solution is to increase a number of the amplicons at the amplification sites. 1. A fluidic system , comprising:a reagent manifold including at least one valve in fluid communication with an inlet port of a flow cell that includes an array of amplification sites, the reagent manifold further including a plurality of channels fluidly connected between the at least one valve and corresponding reagent reservoirs; anda controller including one or more processors, the controller to control the at least one valve and a pump to flow a first solution through the inlet port over the array of amplification sites on the flow cell and to subsequently flow a different, second solution through the inlet port over the array of amplification sites on the flow cell;wherein the first solution includes a number of target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates (NTPs) and one or more replication enzymes, the number of target nucleic acids in the first solution exceeding a number of the amplification sites in the array, the first solution reacting on the flow cell to ...

HIGH EFFICIENCY, SMALL VOLUME NUCLEIC ACID SYNTHESIS

The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes). 1. A method for removing a bead from a fluid-filled well of a microchip for synthesizing nucleic acid molecules , wherein a nucleic acid molecule is attached to the bead , the method comprising:providing a voltage between a first electrode that is arranged at a bottom of the fluid-filled well and a second electrode, wherein the voltage is sufficient to cause fluid in the fluid-filled well to undergo electrolysis producing one or more bubbles in the fluid to rise to a top of the fluid-filled well along with the bead or to lift the bead to the top of the fluid-filled well.2. The method of claim 1 , further comprising collecting the bead that has risen to the top of the fluid-filled well with a bead-collection device.3. The method of claim 2 , further comprising transferring the bead that was collected to a well of a first multiwell collection plate.4. The method of claim 1 , wherein the fluid comprises an aqueous or a non-aqueous buffer solution.5. The method of claim 1 , wherein the fluid comprises water claim 1 , methanol claim 1 , acetonitrile claim 1 , and Net4pTsO.6. The method of claim 1 , wherein the first electrode is composed of platinum and the voltage is about 0.1 to about 100 volts.7. The method of claim 1 , wherein each well of the microchip is individually addressable by a controller.8. The method of claim 1 , wherein the bead is composed of: a synthetic polymer claim 1 , a modified naturally occurring polymer claim 1 , glass claim 1 , controlled pore glass claim 1 , magnetic controlled pore glass claim 1 , magnetic beads claim 1 ...

SAMPLE PROCESSING AND DETECTING KIT WITH A MATERIAL TRANSFER STRUCTURE

A sample processing and detecting kit with a material transfer structure comprises a kit body defined by an outer shell. A top outer shell of the kit body comprises an opening-and-closing sample port. An inner side of the kit body is disposed with a material circular plate holder and a middle of the material circular plate holder is disposed with a circular hole. A waste liquid storage chamber, a first lock groove, a second lock groove, and a sealing hole are arranged on the material circular plate holder at a periphery of the circular hole. A sample processing plastic tube member is hermitically disposed in the first lock groove. A detection reaction plastic tube member is hermetically disposed in the second lock groove. 1. A sample processing and detecting kit with a material transfer structure , comprising: a top outer shell of the kit body comprises an opening-and-closing sample port,', 'an inner side of the kit body is disposed with a material circular plate holder,', 'a middle of the material circular plate holder is disposed with a circular hole,', 'a waste liquid storage chamber, a first lock groove, a second lock groove, and a sealing hole are arranged on the material circular plate holder at a periphery of the circular hole,', 'a sample processing plastic tube member is hermitically disposed in the first lock groove,', 'a detection reaction plastic tube member is hermetically disposed in the second lock groove,', 'the material circular plate holder is rotatably connected to the material transfer structure through the circular hole,', 'the material transfer structure comprises a hollow rotor disposed with a pipette tip,', 'a bottom end of the hollow rotor is disposed with a hollow pointed structure for piercing a sealing film,', 'a rotatable connection structure surrounds a bottom end of the hollow rotor,', 'a silicone seal disposed below the bottom end of the hollow rotor is disposed in the rotatable connection structure,', 'a spring surrounds an outer ...

DEVICES AND METHODS FOR SAMPLE PREPARATION

Methods, apparatuses, and computer program products for preparation of samples for use with in vitro diagnostic tests or analytical assays for detecting one or more analytes. For example, certain embodiments may relate to diagnostic tests performed on human subjects for a variety of applications related to health, medical, and wellness testing. Other embodiments may relate to testing in various applications. Further embodiments may relate to processing environmental samples for detection of various analytes of biological or non-biological origin. Additional embodiments may relate to various applications that require mixing an arbitrary sample with a precise amount of one or more reagents. 1. A sample extraction device for extracting a biological analyte from a biological sampling device , comprising:a sample chamber configured to accept a biological sampling device;a reagent storage vessel, optionally mounted onto an exterior of the sample chamber;a lance mounted inside a lance cavity of the sample extraction device;a mechanism configured to apply compressive or piercing mechanical force on the reagent storage vessel, the lance or the lance cavity, and thereby push the reagent storage vessel against the lance;a housing component enclosing the sample chamber, the reagent storage vessel, the mechanism, and the lance; anda cap covering at least a portion of an opening of the sample chamber, the cap configured to provide controlled release of a liquid sample from the sample chamber.2. The sample extraction device according to claim 1 , wherein the cap comprises one or more partition walls configured to partition between an amount of the liquid sample that will be dispensed from the cap claim 1 , and an amount of the liquid sample that will remain inside the cap and sample extraction device.3. The sample extraction device according to claim 1 , wherein the cap is configured to be adjustable to alter a volume of liquid in the cap depending on a desired liquid sample ...

System and Method for a Pharmaceutical Product

The present disclosure relates to a biological fluid processing system and method. The system comprises a fluid processing device comprising at least one fluid path, a pump for providing a pressure in the at least one fluid path, a valve arranged along said fluid path and a first actuator arranged to control the valve to assume a desired opening state of said fluid path. The biological fluid processing system comprises further a processing interface comprising a pump drive for driving the pump of the fluid processing device, and a processing control element comprising a pump control system arranged to control at least the pump drive and a valve control system arranged to control the first actuator. The system is modular. The fluid processing device is comprised in a fluid processing device module having a predetermined fluid processing device configuration. The processing interfaces have a predetermined processing interface configuration. The processing control element is arranged to receive information relating to the predetermined processing interface configuration of the processing interface module and/or the predetermined fluid processing device configuration of the fluid processing device module and control the at least one pump drive and/or the valve based on the received information relating to the predetermined fluid processing device configuration and the predetermined processing interface configuration. 2. The system according to claim 1 , wherein the processing control element comprises or is connected to a user interface for input of information relating to the predetermined fluid processing device configuration and/or the predetermined processing interface configuration.3. The system according to of the claim 1 , wherein the processing interfaces are comprised in a processing interface module having the predetermined processing interface configuration claim 1 , wherein the processing control element is arranged to receive information relating to the ...

SAMPLE LOADING CARTRIDGE

A sample loading cartridge () for a microfluidic device comprises a cartridge body () with a sample reservoir () configured to house a volume of a liquid sample () and a sample port () in connection with the sample reservoir (). The cartridge () also comprises an output channel () extending from the sample reservoir () and a feedback channel () connected to the sample reservoir () and to the sample port (). The cartridge body () comprises a detection portion () aligned with the feedback channel () to enable detection of any sample () in the feedback channel (). The flow resistance of the feedback channel () is lower than the flow resistance of the output channel () to cause liquid sample () received in the sample port () to enter the feedback channel () with substantially no liquid sample () entering the output channel (). 118.-. (canceled)19. A sample loading cartridge for a microfluidic device , the sample loading cartridge comprising:a cartridge body comprising a sample reservoir configured to house a volume of a liquid sample;a sample port in connection with the sample reservoir and configured to receive the liquid sample;an output channel connected to and extending from the sample reservoir; anda feedback channel connected to the sample reservoir and to the sample port, whereinthe cartridge body comprises a detection portion aligned with at least a portion of the feedback channel to enable detection of presence of liquid sample in the feedback channel; anda flow resistance of the feedback channel is lower than a flow resistance of the output channel to cause liquid sample received in the sample port to enter the feedback channel with substantially no liquid sample entering the output channel.20. The sample loading cartridge according to claim 19 , wherein the detection portion is a window aligned with the at least a portion of the feedback channel to provide visual access to the feedback channel.21. The sample loading cartridge according to claim 19 , wherein ...

APPARATUS AND PLATFORM FOR DETECTION OF ALLERGEN

Provided is an allergen detecting apparatus that can combine with an allergen microarray chip, and the apparatus comprises a microfluidic chip and a clamping unit. The allergen microarray chip can be any commercial chip and a reaction region of the microfluidic chip is configured to fit tightly with a microarray region of the allergen microarray chip allowing a sample or a reagent to contact with a protein allergen disposed on the microarray region. The present invention also provides a platform comprising a control system configured to automatically perform the mixing/binding reactions of the antibody/antigen in the apparatus of the present invention to achieve a faster, more cost-effective, and accurate detection of allergens. 1. An allergen detecting apparatus for incorporating an allergen microarray chip having a microarray region , comprising:a microfluidic chip having an air control layer and a fluid channel layer, wherein the air control layer comprises at least an air control inlet, and the fluid channel layer comprises at least a liquid injection port and a reaction region; anda clamping unit having a housing space for accommodating the microfluidic chip, and at least a clamping member for fixing the microfluidic chip to allow the reaction region to align and tightly fit with the microarray region.2. The allergen detecting apparatus according to claim 1 , wherein an area of the reaction region is not less than that of the microarray region.3. The allergen detecting apparatus according to claim 1 , wherein the clamping unit comprises a cover and at least one clamping member claim 1 , and the housing space is formed by the cover.4. The allergen detecting apparatus according to claim 1 , wherein the microfluidic chip further comprises a leakage-proof wall disposed in the fluid channel layer to prevent leakage when the microfluidic chip is incorporated with the allergen micro array chip.5. The allergen detecting apparatus according to claim 1 , wherein the ...

Diagnostic device

A microfluidic diagnostic device may comprise a fluid inlet to receive a fluid from a fluidic slot, a main microfluidic channel fluidly coupled to the fluid inlet, and a main microfluidic pump interposed between the fluid inlet and the main microfluidic channel to continuously circulate a fluid through the fluidic slot, fluid inlet, and main microfluidic channel wherein the width of the fluid inlet is different from the width of the main microfluidic channel. A diagnostic device, comprising a fluidic slot, a fluid inlet fluidly coupled to the fluidic slot, a main channel fluidly coupled to the fluid inlet, and an inlet pump interposed between the fluid inlet and channel wherein the cross-sectional area of the fluid inlet is relatively larger at least one point than the cross-sectional area of the channel.

Removing bubbles in a microfluidic device

Methods of removing bubbles from a microfluidic device are described where the flow is not stopped. Methods are described that combine pressure and flow to remove bubbles from a microfluidic device. Bubbles can be removed even where the device is made of a polymer that is largely gas impermeable.

Microfluidic Aliquoting For Single-Cell Isolation

According to the invention, generally, a microfluidic aliquoting (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. Design improvements including an injection mold design for an insert and a base and a multiplex hole punch allow for rapid fabrication of the MA chip. 1. A microfluidic aliquot (MA) chip for performing single-cell isolation , comprising:a chip having a radius, a center, a top surface, a bottom surface, an outer edge and a thickness;a single center inlet well disposed substantially at the center of the chip, extending into and accessible from the top surface of the chip;a plurality of side outlet wells disposed in an annular outer portion of the chip, extending into and accessible from the top surface of the chip; anda plurality of channels having multiple segments that extend into the bottom surface of the chip and extending from the center inlet well to the side outlet wells in fluid communication with the center inlet well and the side outlet wells, configured to maintain uniform distribution of a liquid from the center inlet well to the side outlet wells.2. The MA chip of claim 1 , wherein the multiple segments comprise:a plurality of first segments that form an inner layer, a plurality of last segments that form an outer layer, and a plurality of segments between the first segments and the last segments form a middle layer;wherein the side outlet wells and the multiple segments are configured to uniformly distribute liquid and a plurality of cells when the liquid and cells are placed in the MA chip;wherein the first segment is connected to the center inlet well in a radial pattern; andwherein each segment after the first ...

INSTRUMENT FOR PERFORMING A DIAGNOSTIC TEST ON A FLUIDIC CARTRIDGE

A cartridge reader is configured to carry out a diagnostic test on a fluid sample contained within a fluidic cartridge. The cartridge comprises first, second and third collapsible blisters containing at least one reagent for use in the diagnostic test. The cartridge reader comprises an upper clamp, occupying a fixed position relative to the reader and a lower clamp, movable relative to the upper clamp, and wherein the upper clamp and the lower clamp are configured to receive and hold a fluidic cartridge therebetween. First, second and third blister actuators are mounted on the upper clamp, for aligning with first, second and third collapsible blisters of a fluidic cartridge inserted into the reader. The first, second and third blister actuators are movable relative to the upper clamp, between a first position in which the blister actuators are spaced apart from the collapsible blisters comprised on the fluidic cartridge received between the upper and lower clamps, and a second position in which the blister actuators depress the collapsible blisters, thereby collapsing the blisters and ejecting the reagents contained therein into a channel in the microfluidic cartridge. 1. A cartridge reader configured to carry out a diagnostic test on a fluid sample contained within a fluidic cartridge , wherein the cartridge comprises first , second and third collapsible blisters containing at least one reagent for use in the diagnostic test , the cartridge reader comprising:an upper clamp, occupying a fixed position relative to the reader and a lower clamp, movable relative to the upper clamp, and wherein the upper clamp and the lower clamp are configured to receive and hold a fluidic cartridge therebetween; andfirst, second and third blister actuators mounted on the upper clamp, and for aligning with first, second and third collapsible blisters of a fluidic cartridge inserted into the reader, wherein the first, second and third blister actuators are movable relative to the upper ...

INSTRUMENT FOR PERFORMING A DIAGNOSTIC TEST ON A FLUIDIC CARTRIDGE

A cartridge reader controlled by processing means for carrying out a diagnostic test on a sample contained in a fluidic cartridge comprises a mechanical valve for isolating the sample with the cartridge. A system for actuating the mechanical valve comprises an actuation member configured to move the mechanical valve from an open position to a closed position and an armature connected to the actuation member. The armature is configured to engage an electromagnet, wherein the electromagnet can be switched between an active state in which it electromagnetically holds the armature and an inactive state in which it does not electromagnetically hold the armature. First biasing means are disposed between the actuation member and a bearing surface, wherein the first biasing means is configured to bias the actuation member into a first position in which it actuates a mechanical valve in a fluidic cartridge inserted into the reader. 1. A cartridge reader for carrying out a diagnostic test on a sample contained in a fluidic cartridge comprising a mechanical valve for isolating the sample with the cartridge , the cartridge reader comprising a system for actuating the mechanical valve comprising:an actuation member configured to move the mechanical valve from an open position to a closed position;an armature connected to the actuation member, configured to engage an electromagnet, wherein the electromagnet can be switched between an active state in which it electromagnetically holds the armature and an inactive state in which it does not electromagnetically hold the armature;first biasing means, disposed between the actuation member and a bearing surface, wherein the first biasing means is configured to bias the actuation member into a first position in which it actuates a mechanical valve in a fluidic cartridge inserted into the reader;wherein the actuation member is held in a second position against the bias of the first biasing means by the electromagnet when the ...

MICROFLUIDIC DEVICES FOR THE RAPID AND AUTOMATED PROCESSING OF SAMPLE POPULATIONS

Microfluidic devices for the rapid and automated processing of sample populations are provided. Described are multiplexer tiplexer microfluidic devices configured to serially deliver a plurality of distinct sample populations to a sample processing element rapidly and automatically, without cross-contaminating the distinct sample populations. Also provided are microfluidic sample processing elements that can be used to rapidly and automatically manipulate and/or interrogate members of a sample population. The microfluidic devices can be used to improve the throughput and quality of experiments involving model organisms, such as

EXTENDED RANGE IMMUNOASSAY DEVICES WITH IMMUNOSENSOR AND MAGNETIC IMMUNOSENSOR

The present invention relates to systems and methods that utilize a combination of immunoassay and magnetic immunoassay techniques to detect an analyte within an extended range of specified concentrations. In particular, a device includes a housing, a heterogeneous surface capture immunosensor within the housing and configured to generate a first signal indicative of the concentration of the analyte in an upper concentration range, and a homogeneous magnetic bead capture immunosensor within the housing and configured to generate a second signal indicative of the concentration of the analyte in a lower concentration range. 1. A sensor chip comprising:a non-conductive substrate;a first sensor for an analyte; anda second sensor for the analyte,wherein the non-conductive substrate is planar, and comprises a top surface and a bottom surface, the top surface forming a base for the first sensor and the second sensor,wherein the first sensor is a heterogeneous surface capture immunosensor comprising a conductive layer deposited on the non-conductive substrate, and a capture biomolecule immobilized on or near at least a portion the conductive layer, andwherein the second sensor is a homogeneous magnetic bead capture immunosensor comprising a second microelectrode, and a magnetic element.2. The sensor chip of claim 1 , wherein the first sensor and the second sensor are spaced at least 0.3 mm apart on the non-conductive substrate.3. The sensor chip of claim 1 , wherein the capture biomolecule is coated onto microspheres deposited on the at least the portion of the conductive layer.4. The sensor chip of claim 1 , wherein the capture biomolecule is an antibody.5. The sensor chip of claim 4 , wherein the antibody is an anti-troponin I (TnI) antibody or an anti-cardiac troponin I (cTnI) antibody.6. The sensor chip of claim 1 , wherein the at least the portion of the conductive layer is surrounded by a containment structure.7. The sensor chip of claim 1 , wherein the magnetic ...

Removing bubbles in a microfluidic device

Methods of removing bubbles from a microfluidic device are described where the flow is not stopped. Methods are described that combine pressure and flow to remove bubbles from a microfluidic device. Bubbles can be removed even where the device is made of a polymer that is largely gas impermeable.

Removing bubbles in a microfluidic device

Methods of removing bubbles from a microfluidic device are described where the flow is not stopped. Methods are described that combine pressure and flow to remove bubbles from a microfluidic device. Bubbles can be removed even where the device is made of a polymer that is largely gas impermeable.

BIOMOLECULE ISOLATION

Methods, devices and systems for handling sample liquids, encapsulating liquids and magnetic particles are disclosed. 119-. (canceled)20. A liquid handling system comprising:a conduit having a predetermined trapping site,a pump configured to apply positive pressure, negative pressure, or no external pressure to a location in the conduit,a magnetic field source configured to apply a magnetic field at the trapping site when activated and substantially no magnetic field when not activated, anda controller operably attached to the pump and the magnetic field source so that the controller can activate the pump and/or the magnetic field source, the controller being programmed to:activate the pump so that an encapsulating liquid is flowed in the conduit;activate the pump so that a sample liquid is flowed in the conduit in such a way that the sample liquid is (a) surrounded by the encapsulating liquid and (b) located at the trapping site within the conduit, the sample liquid containing magnetic particles;activate the magnetic field source so that the magnetic particles are immobilized at the trapping site; andactivate the pump so that an elution liquid is flowed in the conduit in such a way that (a) the elution liquid is surrounded by the encapsulating liquid, (b) the sample liquid is flowed away from the trapping site, and (c) the elution liquid is flowed to the trapping site and surrounds the magnetic particles.21. The system of wherein the conduit is a capillary tube.22. The system of wherein the encapsulating liquid claim 20 , sample liquid and elution liquid are flowed by negative pressure applied by the pump to the conduit.23. The system of wherein the encapsulating liquid claim 20 , sample liquid and elution liquid are flowed by positive pressure applied by the pump to the conduit.24. A system for handling a first sample liquid containing magnetic particles claim 20 , a second sample liquid claim 20 , and an encapsulating liquid claim 20 , both sample liquids being ...

Systems and Methods for Multiple Analyte Detection

Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials. 1. A system for distribution of a biological sample , the system comprising:a substrate, wherein the substrate comprises a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber;a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate; anda sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber,wherein the substrate is constructed of detection-compatible and assay-compatible materials.2. The system of claim 1 , wherein the sealing instrument is configured to be placed in contact with an exterior portion of the substrate.3. (canceled)4. (canceled)5. The system of claim 1 , wherein the sealing instrument is configured to be placed in contact with the substrate at least one of before a reaction process that occurs in the sample chambers and during a reaction process that occurs in the sample chambers.6. The system of claim 5 , wherein the sealing plate is configured to be placed in contact ...

Sieve Valves, Microfluidic Circuits, Microfluidic Devices, Kits, and Methods for Isolating an Analyte

The invention generally provides a sieve valve including: a substrate defining a channel; a flexible membrane adapted and configured for deployment at an intersection with the channel; and one or more protrusions extending into the channel from the substrate or the flexible membrane. The one or more protrusions define a plurality of recesses extending beyond the intersection between the channel and the flexible membrane; 1. A method of isolating an analyte , the method comprising:loading a sample into a holding chamber of a microfluidic circuit, the microfluidic circuit including one or more sieve valves, wherein the holding chamber is in fluid communication with an input port of a mixing circuit,capturing an analyte on a capture substrate in the mixing circuit;washing the capture substrate to remove uncaptured components; and a substrate defining a channel;', 'a flexible membrane adapted and configured for deployment at an intersection with the channel; and', 'one or more protrusions extending into the channel from the substrate or the flexible membrane, the one or more protrusions defining a plurality of recesses extending beyond the intersection between the channel and the flexible membrane; and, 'releasing the analyte from the capture substrate, wherein the sieve valve comprises'}passing the sample into the mixing circuit.2. The method of claim 1 , wherein the capture substrate is loaded into the microfluidic circuit claim 1 , the holding chamber claim 1 , and/or the mixing circuit.3. The method of claim 1 , wherein the capture substrate comprises a bead claim 1 , microbead claim 1 , surface of the microfluidic circuit claim 1 , or a capture reagent.4. The method of claim 1 , wherein the mixing circuit comprises a plurality of chambers in fluid communication with one or more holding chambers claim 1 , wherein the one or more holding chambers and/or the mixing circuit comprises the capture substrate for isolating the analyte.5. The method of claim 4 , wherein the ...

PORTABLE ALLERGEN DETECTION SYSTEM

The present invention provides systems, devices and methods for detecting the presence and/or absence of one or more allergens in a sample particularly a food sample. The detection system includes a separate sample pickup, one or more disposables for receiving and processing a test sample and a detection device that can execute an allergen detection testing in minutes. The present detection system and device is compact and portable. 1. A portable allergen detection system comprising:a cup body;a cup lid assembly having a plurality of reaction chambers, at least one port opening into the cup body, and a flow tube with a distal end extending into the cup body;a sampler; anda housing, having a detection device.2. The portable allergen detection system in claim 1 , wherein the at least one port further comprises a food corer port and a rotor port; the food corer port for receiving the sample and the rotor port for receiving a rotor.3. The portable allergen detection system in claim 1 , wherein the cup body is in fluid communication with the detection device.4. The portable allergen detection system in claim 2 , wherein the flow tube is in fluid communication with and between the cup body and the detection device.5. The portable allergen detection system in claim 4 , wherein the flow tube is connected distally to a filter assembly claim 4 , which is in fluid communication with the detection device.6. The portable allergen detection system of claim 1 , wherein the at least one port includes three ports; the three ports comprising a rotor port claim 1 , a food corer port claim 1 , and a test cup port.7. The portable allergen detection system of further comprising a flow controlling component claim 6 , and wherein the test cup port connects the cup body to the flow controlling component.8. The portable allergen detection system of claim 7 , wherein the flow controlling component is within the detection device claim 7 , and wherein the flow controlling component is capable ...

MICROFLUIDIC DEVICE

A microfluidic device () for separating a liquid L into first and second liquid components L, Lthereof is described. The microfluidic device () comprises an inlet () for receiving the liquid therethrough. The microfluidic device () comprises a first outlet () for the first liquid component L, wherein the first outlet () is fluidically coupled to the inlet () via a first passageway (). The microfluidic device () comprises a second outlet () for the second liquid component L, wherein the second outlet () is fluidically coupled to the first passageway (A) via a first set of N conduits (A, B, C, D, E), wherein N is a positive integer greater than 1, wherein respective conduits A, B, C, D, E of the first set of N conduits divide from the first passageway A at respective divisions (A, B, C, D, E) from the inlet therealong The respective conduits A, B, C, D, E of the first set of N conduits are arranged to, at least in part, equalize flowrate ratios at the respective divisions (A, B, C, D, E). 120-. (canceled)21. A microfluidic device for separating a liquid into first and second liquid components thereof , the microfluidic device comprising:an inlet for receiving the liquid therethrough;a first outlet for the first liquid component, wherein the first outlet is fluidically coupled to the inlet via a first passageway; anda second outlet for the second liquid component, wherein the second outlet is fluidically coupled to the first passageway via a first set of N conduits, wherein N is a positive integer greater than 1, wherein respective conduits of the first set of N conduits divide from the first passageway at respective divisions from the inlet there-along, the respective conduits of the first set of N conduits are arranged to, at least in part, equalize flowrate ratios at the respective divisions;', 'the first passageway comprises a set of expansion members; and', 'respective expansion members of the set of expansion members correspond with the respective conduits of the ...

METHODS AND DEVICES FOR SEQUENCING NUCLEIC ACIDS IN SMALLER BATCHES

The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency. 121-. (canceled)22. An automated nucleic acid sequencing system , comprising:a) flow cells comprising nucleic acid to be sequenced, said flow cells mounted onb) a moveable support controlled by ac) software user interface, said support configured to move flow cells toc) reagent delivery stations, each reagent delivery station in fluidic communication withd) reagent reservoirs configured to supply extension reagents including fluorescent nucleotide analogues, ande) an imaging platform configured to detect said fluorescent nucleotide analogues.23. The system of claim 22 , wherein said imaging platform is associated with a camera claim 22 , said camera positioned for imaging one or more flow cells.24. The system of claim 23 , wherein said imaging platform is configured for x/y movement and said imaging platform is positioned under said camera.25. The system of claim 22 , wherein said system is contained within the housing of an instrument claim 22 , said software user interface mounted on said housing claim 22 , said software user interface in communication with a programmable processor.26. The system of claim 22 , wherein said moveable support is a carousel.27. The system of claim 22 , further comprising an actuator configured for raising said moveable support so as to disconnect said flow cells from said stations.28. The system of claim 22 , further comprising a transfer means configured for moving at least one of said flow cells from said moveable support to said imaging platform. The invention relates to methods, ...