SELF-CONTAINED BIOLOGICAL ASSAY APPARATUS, METHODS, AND APPLICATIONS

A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.

Self-contained biological assay apparatus, methods, and applications

A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.

Self-contained biological assay apparatus, methods, and applications

A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.

Self-contained biological assay apparatus, methods, and applications

A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.

SELF-CONTAINED BIOLOGICAL ASSAY APPARATUS, METHODS, AND APPLICATIONS

A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing. 1. A biological assay system , the system comprising:an optical unit comprising a light source and a detector; and a housing;', 'a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing;', 'a reagent supply component disposed in the housing;', 'a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform;', 'a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and', 'a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing,', 'wherein the apparatus is characterized as a self-contained, fully automated biological assay-performing apparatus;, 'a biological assay apparatus comprisingwherein the light source is configured to ...

Self-contained Biological Assay Apparatus, Methods, and Applications

A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.

SELF-CONTAINED BIOLOGICAL ASSAY APPARATUS, METHODS, AND APPLICATIONS

A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing. 1. An automated process for isolating , amplifying , and analyzing a target nucleic acid sequence that may be present in a fluid test sample , comprising:providing a pneumatic manifold that operates a microfluidic system having a fluidic transport layer and a fluidic channel disposed therein, and reservoirs attached thereto;introducing the fluid test sample into the fluidic channel;providing at least one reagent to the channel from at least one respective reservoir that is in fluid connection with the fluidic transport layer;combining the fluid test sample and the at least one reagent in a region of the fluidic transport layer, reservoir or amplification reactor;transporting the fluid test sample to a temperature-controlled amplification/reaction reactor that is in operative communication with the fluidic transport layer;incubating the fluid test sample in the amplification/reaction reactor under conditions sufficient to permit the target nucleic acid sequence to be amplified;transporting the fluid test sample to an analysis reservoir; andanalyzing the amplified target nucleic acid sequence from the test sample ...

Self-contained biological assay apparatus, methods, and applications

A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.

SELF-CONTAINED BIOLOGICAL ASSAY APPARATUS, METHODS, AND APPLICATIONS

A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.

Integrated microfluidic device and methods

A microfluidic device for analyzing a sample of interest is provided. The microfluidic device can comprise a microfluidic device body, wherein the microfluidic device body comprises a sample preparation area, a nucleic acid amplification area, a nucleic acid analysis area, and a network of fluid channels. Each of the sample preparation area, the nucleic acid amplification area and the nucleic acid analysis area are fluidly interconnected to at least one of the other two areas by at least one of the fluid channels. Using the microfluidic device, sample preparation can be combined with amplification of a biologically active molecule, and a suitable biological sample can be provided for analysis and/or detection of a molecule of interest. The small-scale apparatus and methods provided are easier, faster, less expensive, and equally efficacious compared to larger scale equipment for the preparation and analysis of a biological sample.

Mechanically actuated diagnostic device

Disclosed are mechanically-actuated devices for transporting fluids within a microfluidic circuit and performing diagnostic operations on a sample. Also disclosed are related methods for performing sample analysis effected by the motion of an actuator proximate to a microfluidic system.

Self-contained biological assay apparatus, methods, and applications

A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.

Self-contained biological assay apparatus, methods, and applications

A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.

MECHANICALLY ACTUATED DIAGNOSTIC DEVICE

Disclosed are mechanically-actuated devices for transporting fluids within a microfluidic circuit and performing diagnostic operations on a sample. Also disclosed are related methods for performing sample analysis effected by the motion of an actuator proximate to a microfluidic system.

SELF-CONTAINED BIOLOGICAL ASSAY APPARATUS, METHODS, AND APPLICATIONS

A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.

Integrated Microfluidic Device and Methods

A microfluidic device for analyzing a sample of interest is provided. The microfluidic device can comprise a microfluidic device body, wherein the microfluidic device body comprises a sample preparation area, a nucleic acid amplification area, a nucleic acid analysis area, and a network of fluid channels. Each of the sample preparation area, the nucleic acid amplification area and the nucleic acid analysis area are fluidly interconnected to at least one of the other two areas by at least one of the fluid channels. Using the microfluidic device, sample preparation can be combined with amplification of a biologically active molecule, and a suitable biological sample can be provided for analysis and/or detection of a molecule of interest. The small-scale apparatus and methods provided are easier, faster, less expensive, and equally efficacious compared to larger scale equipment for the preparation and analysis of a biological sample.

Systems and Methods for Capturing Full Resolution Ion Mobility Data and Performing Multi-Analyte Targeted Data Acquisition

A system for capturing full resolution ion mobility data includes an ion mobility separation device that receives ions, guides a portion thereof, and separates the guided ions based on mobility. An ion detector receives the ions, detects ions having a predefined mass-to-charge ratio during a time period, and generates a responsive signal(s). A data acquisition system receives the signal(s) from the detector and generates a time dependent ion signal representing intensity of the signal(s) at different arrival time periods during the time period. A system for performing multi-analyte targeted data acquisition includes an ion mobility separation device that receives a stream of ions, guides a portion of the ions, and separates the ions based on mobility. An ion detector receives the ions and switches from detecting a first mass-to-charge ratio during a first arrival time to detecting a second mass-to-charge ratio during a second arrival time of a first scan.

Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease

Biomarkers for the detection of pre-cancer and cancer cells and a method for their detection at the point of care are disclosed. The system is comprised of an inexpensive, disposable microfluidic cassette for sample preparation; continuous-flow, magnetic-bead assisted cell sorting and cytometry; cell lysis; nucleic acid isolation and amplification, hybridization, quantification, and analysis. Typically, the disposable cassette will be used with a benchtop instrument platform that provides power, control and detection capabilities to the cassette. In one particular embodiment and application of the invention, the cell sorting is based on the selective binding of magnetic beads coated with an MCAP antibody to exfoliated precancerous and cancerous cells contained in saliva. The bead-labeled precancerous and cancerous cells are then separated from other saliva components using a magnetic field. Both labeled and unlabeled cells can be counted using integrated detectors of several types, including cell counters based on impedance measurements, and cytometers based on Coulter particle counter principles. The sorted sub-population of precancerous and cancerous cells are lysed, and the nucleic acids are isolated for gene assays and/or transcription profiling of the sorted sample subpopulation. In one embodiment of the invention, the cassette includes re verse- transcription polymerase chain reaction for nucleic acid detection and a hybridization array for gene identification. The devices, systems, modes of operation, and methods disclosed here are also applicable for cancer and other disease detection in other body fluids.

Methods of testing using a microfluidic cassette

The present invention relates to sample processing using a microfluidic chip. A method for concurrent testing for at least two of RNA, DNA, antibody, and antigen in a sample is described, as are methods for testing for pre-selected pathogens and microfluidic methods.

Methods of testing using a microfluidic cassette

The present invention relates to sample processing using a microfluidic chip. A method for concurrent testing for at least two of RNA, DNA, antibody, and antigen in a sample is described, as are methods for testing for pre-selected pathogens and microfluidic methods.

System for testing

The present invention relates to sample processing using a microfluidic chip. Systems are described comprising a cassette having at least one port and a sample inlet in fluid communication with a detection zone for interacting with pre-selected RNA sequences, DNA sequences, antibodies, or antigens, or mixtures thereof, if present, in a sample; and a developer for engaging the port of the cassette, wherein the developer propels the sample from said inlet to said detection zone.

Microfluidic chip

The present invention relates to sample processing using a microfluidic chip. The chip contains at least two detection zones for interacting with pre-selected RNA sequences, DNA sequences, antibodies, or antigens to determine their presence in the sample. Chips with certain microfluidic features are also described.

Systems and methods for capturing full resolution ion mobility data and performing multi-analyte targeted data acquisition

A system for capturing full resolution ion mobility data includes an ion mobility separation device that receives ions, guides a portion thereof, and separates the guided ions based on mobility. An ion detector receives the ions, detects ions having a predefined mass-to-charge ratio during a time period, and generates a responsive signal(s). A data acquisition system receives the signal(s) from the detector and generates a time dependent ion signal representing intensity of the signal(s) at different arrival time periods during the time period. A system for performing multi- analyte targeted data acquisition includes an ion mobility separation device that receives a stream of ions, guides a portion of the ions, and separates the ions based on mobility. An ion detector receives the ions and switches from detecting a first mass-to-charge ratio during a first arrival time to detecting a second mass-to-charge ratio during a second arrival time of a first scan.

Systems and methods for capturing full resolution ion mobility data and performing multi-analyte targeted data acquisition