27-03-2014 дата публикации
Номер: US20140087446A1
The present invention relates to methods for culturing spirochetes, in particular . The present invention also provides methods of identifying spirochetes present in a biological sample. The present invention further provides methods of diagnosing diseases cause by a spirochete infection, such as Lyme disease, syphilis, and multiple sclerosis. The present invention further provides methods for identifying spirochete susceptibilities to antimicrobials and antimicrobial compositions and cocktails. The present invention also provides methods for treating subjects suspected of having a spirochete infection. 1. An in vitro method of culturing a spirochete comprising:(1) inoculating a spirochete culture medium with the spirochete to generate a first culture;{'sub': '2', '(2) incubating the first culture in a first culture vessel in about 3-5% COfor about 4-6 days;'}(3) optionally evaluating the first culture for the presence of spirochetes; and{'sub': 2', '2, '(4) after incubating the first culture in about 3-5% COfor about 4-6 days, contacting the COincubated first culture with a matrix-coated support,'}{'i': 'Borrelia.', 'wherein the spirochete is a species of'}2Borrelia afzelii, Borrelia anserine, Borrelia burgdorferi, Borrelia garinii, Borrelia hermsii, Borrelia recurrentis, Borrelia valaisianaBorrelia vincentii.. The method according to claim 1 , wherein the spirochete is claim 1 , or3. The method according to claim 1 , wherein the spirochete culture medium comprises a spirochete-supporting medium claim 1 , serum claim 1 , a reducing agent claim 1 , and an antibiotic.4. The method according to claim 3 , wherein the spirochete-supporting medium is selected from BSK-H medium and BSK-II medium.5. The method according to claim 3 , wherein the serum is rabbit serum at a concentration of about 6-12%.6. The method according to claim 5 , wherein the rabbit serum concentration is 12%.7. The method according to claim 3 , wherein the reducing agent is dithiothreitol (DTT) claim ...
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