02-05-2019 дата публикации
Номер: US20190127727A1
Принадлежит:
The present invention provides a method for extracting polyhydroxyalkanoates (PHAs), which comprises a pre-process step and an extraction step: removing water from waste sludge containing microorganisms in the pre-process step so that the waste sludge containing microorganisms has a water content of less than 40%; and applying a high-voltage pulsed electric field to the waste sludge during the extraction step to destroy the microorganisms and release the PHAs, wherein the high-voltage pulsed electric field is between 50 volts and 400 volts, an application time of the high-voltage pulsed electric field is between 5 seconds and 90 seconds, and an application frequency of the high-voltage pulsed electric field is between 500 Hz and 1000 Hz, thereby extracting the PHAs in the case of few chemicals. 1. A method for extracting polyhydroxyalkanoates (PHAs) , comprising the following steps:a pre-process step: removing water from waste sludge containing microorganisms so that the waste sludge containing microorganisms has a water content of less than 40%; andan extraction step: by utilizing a electroporation, applying a high-voltage pulsed electric field to the waste sludge to destroy the microorganisms and release polyhydroxyalkanoates (PHAs), wherein the high-voltage pulsed electric field is between 50 volts and 400 volts, an application time of the high-voltage pulsed electric field is between 5 seconds and 90 seconds, and an application frequency of the high-voltage pulsed electric field is between 500 Hz and 1000 Hz.2. The method of claim 1 , the high-voltage pulsed electric field is applied to the waste sludge for 15 seconds to 60 seconds at a frequency of 100 volts to 400 volts and at a frequency of 500 to 1000 Hz.3. The method of claim 1 , the extraction step further comprising a chemical extraction process claim 1 , wherein the waste sludge is subjected to a primary extraction by the electroporation claim 1 , and then subjected to the second extraction by the ...
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