Peptides capable of binding to serum proteins and compounds, constructs and polypeptides comprising the same

The present invention relates to amino acid sequences that are capable of binding to serum proteins; to compounds, proteins, polypeptides, fusion proteins or constructs comprising or essentially consisting of such amino acid sequences; to nucleic acids that encode such amino acid sequences, compounds, proteins, polypeptides, fusion proteins or constructs; to compositions, and in particular pharmaceutical compositions, that comprise such amino acid sequences, compounds, proteins, polypeptides, fusion proteins or constructs; and to uses of such amino acid sequences, compounds, proteins, polypeptides, fusion proteins or constructs.

SPECIFIC DELIVERY OF AGROCHEMICALS

Described is the specific delivery of agrochemicals to plants. More specifically, described is a composition having a targeting agent comprising at least one binding domain that specifically binds to a binding site on an intact living plant and an agrochemical or a combination of agrochemicals. Also described is a binding domain that specifically binds the binding site on an intact living plant. More specifically, described are binding domains comprising a peptide sequence that comprises four framework regions and three complementary-determining regions, or any suitable fragment thereof, so that the binding domains are able to bind or retain a carrier onto a plant. Described are binding domains that specifically bind trichomes, stomata, cuticle, lenticels, thorns, spines, root hairs, or wax layer. Described is a method for delivery of agrochemicals to a plant, for improving the deposition of agrochemicals on a plant, and for retaining the agrochemicals on a plant, using targeting agents comprising the binding domains, and to a method for protecting a plant against biotic or abiotic stress or controlling plant growth using the same. Also, described is a method for manufacturing a specifically targeting agrochemical carrier. 1. A process for producing specifically targeting microcapsules , the process comprising:emulsifying into a continuous aqueous phase an organic phase in which a to be encapsulated agrochemical or combination of agrochemicals is dissolved or dispersed to form an emulsion of droplets of the organic phase in the continuous aqueous phase;causing an aqueous suspension of microcapsules with polymer walls having anchor groups at their surface to be formed; andcovalently linking at least one targeting agent to the anchor groups at the microcapsule surface, at a ratio from about 0.01 μg to about 1 μg targeting agent per square centimeter microcapsular surface.2. The process of claim 1 , further comprising:causing polymerization of polyfunctional monomers ...

INSECT BINDING ANTIBODIES

Described is a binding domain, preferably an antigen binding domain, more preferably an antigen binding domain that specifically binds a binding site on an insect. More specifically, described are antigen binding domains comprising an amino acid sequence that comprises 4 framework regions and 3 complementary determining regions, whereby the antigen binding domains are capable to bind specifically to an insect as a whole, preferably to a binding site on the insect surface. Further described is the use of the binding domain to deliver a compound, preferably a biologically active agent to the insect, and to insecticidal compositions comprising the binding domain. 1. A binding domain able to bind a binding site on an insect.2. The binding domain of claim 1 , wherein the binding site is situated on the surface of the insect.3. The binding domain according to claim 2 , wherein said binding site is situated on the insect exoskeleton.4. The binding domain of claim 2 , wherein the binding site is comprised in an insect structure selected from the group consisting of head claim 2 , thorax claim 2 , abdomen claim 2 , trachea claim 2 , spiracles claim 2 , antennae claim 2 , legs claim 2 , claws claim 2 , wings claim 2 , wingshells claim 2 , mouthparts claim 2 , eyes of the insect or any combination thereof.5. The binding domain of claim 1 , wherein the binding domain comprises a peptide comprising four (4) framework regions and three (3) complementary determining regions claim 1 , or any suitable fragment thereof.6. The binding domain of claim 5 , wherein the binding domain is from a camelid antibody.7. The binding domain according to claim 6 , wherein said binding domain is comprised in a VHH sequence.8. The binding domain of claim 7 , wherein the VHH comprises one of SEQ ID NO:1 through SEQ ID NO:5.9. The binding domain of able to retain a carrier on an insect.10. The binding domain of claim 9 , wherein the binding domain comprises a peptide comprising four (4) framework ...

Compositions for seed treatment

Described is a composition for seed treatment comprising a plant seed-binding protein, preferably an antigen-binding plant seed-binding protein. In one embodiment, the seed-binding protein is binding a polysaccharide, preferably pectin. Further described is the use of a plant seed-binding protein to bind a plant-enhancing agent to a plant seed, and to a method for treating plant seeds.

Chitinous polysaccharide antigen-binding proteins

Described is an antigen-binding protein, preferably comprising an amino acid sequence that comprises four framework regions and three complementarity-determining regions, wherein the antigen-binding protein is capable of binding a chitinous polysaccharide, and uses thereof.

AGROCHEMICAL COMPOSITIONS COMPRISING ANTIBODIES BINDING TO SPHINGOLIPIDS

This disclosure relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one polypeptide, which specifically binds to a pest. The disclosure further provides methods for protecting or treating a plant or a part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of applying directly or indirectly to a plant or to a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or a part of a plant against a infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, to polypeptides with a specific pesticidal activity comprised within an agrochemical formulation, to nucleic acids encoding such polypeptide and to plants comprising chimeric genes comprising such nucleic acids. 115.-. (canceled)16. A method of protecting or treating a plant or plant part , a human or animal , or an object from a biological interaction with a fungal pest or of killing or inhibiting the growth of a fungal pest , the method comprising:applying to a plant or plant part, administering to a human or animal, or applying to an object an antifungal composition comprising at least one polypeptide that specifically binds to a glucosylceramide of a fungal pest, wherein the at least one polypeptide is a heavy chain variable domain of a heavy chain antibody (VHH) or a functional fragment thereof, or wherein the at least one polypeptide is an alphabody or a functional fragment thereof.17. The method according to claim 16 , wherein the composition is administered or applied under conditions effective to protect or treat the plant or a harvested part of the plant claim 16 , the human or animal claim 16 , or the object against the infection or biological interaction with the fungal pest.18. The method according to claim 16 , wherein the plant ...

SPECIFIC DELIVERY OF AGROCHEMICALS

Described is the specific delivery of agrochemicals to plants. More specifically, a targeting agent has at least one binding domain that specifically binds to a binding site on an intact living plant. Such binding domains include a peptide having 4 framework regions and 3 complementary determining regions, or fragment(s) thereof, wherein the binding domains bind or retain a carrier onto a plant. Described are binding domains that specifically bind trichomes, stomata, cuticle, lenticels, thorns, spines, root hairs, or wax layer. Further described are methods for delivering agrochemicals to a plant, for depositing agrochemicals on a plant, and for retaining the agrochemicals on a plant, using targeting agents comprising the binding domains, and to methods for protecting a plant against stress or controlling plant growth. Also, described are methods for manufacturing a specifically targeting agrochemical carrier. 1. A binding domain able to bind at least one binding site on an intact living plant.2. The binding domain of claim 1 , wherein the binding domain is derived from an innate or adaptive immune system.3. A binding domain able to bind at least one binding site on a plant claim 1 , wherein the binding domain is comprised in a peptide that comprises four framework regions and three complementary determining regions claim 1 , or any suitable fragment thereof.4. The binding domain of claim 1 , wherein the binding domain is able to bind a carrier onto a plant.5. The binding domain of claim 1 , wherein the binding domain is able to retain a carrier onto a plant.6. The binding domain of claim 1 , wherein the binding domain binds to a specific part of the plant.7. The binding domain of claim 6 , wherein the part of the plant is selected from the group consisting of leaves claim 6 , stem claim 6 , roots claim 6 , fruits claim 6 , cones claim 6 , flowers claim 6 , bulbs and tubers.8. The binding domain of claim 1 , wherein the binding domain binds to a specific structure ...

AGROCHEMICAL COMPOSITIONS COMPRISING ANTIBODIES BINDING TO SPHINGOLIPIDS

The present invention relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one polypeptide, which specifically binds to a pest. The invention further provides methods for protecting or treating a plant or a part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of applying directly or indirectly to a plant or to a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or a part of a plant against a infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, to polypeptides with a specific pesticidal activity comprised within an agrochemical formulation, to nucleic acids encoding such polypeptide and to plants comprising chimeric genes comprising such nucleic acids. 1. A composition comprising at least one polypeptide that specifically binds to a sphingolipid of a fungal pest.2. (canceled)3. The composition of claim 1 , wherein said at least one polypeptide is a heavy chain variable domain of a heavy chain antibody (V) or a functional fragment thereof claim 1 , or wherein the at least one polypeptide is an alphabody or a functional fragment thereof.45.-. (canceled)6. The composition of claim 1 , wherein the sphingolipid is glucosylceramide.7. The composition of claim 1 , which further comprises an agrochemically or pharmaceutically suitable carrier and/or one or more suitable adjuvants.8. The composition of claim 1 , wherein said pest is a pathogenic fungus.9Alternaria, Ascochyta, Botrytis, Cercospora, Colletotrichum, Diplodia, Erysiphe, Fusarium, Leptosphaeria, Gaeumanomyces, Helminthosporium, Macrophomina, Nectria, Penicillium, Peronospora, Phoma, Phymatotrichum, Phytophthora, Plasmopara, Podosphaera, Puccinia, Pyrenophora, Pyricularia, Pythium, Rhizoctonia, Scerotium, Sclerotinia, Septoria, ...

MANUFACTURING OF SPECIFICALLY TARGETING MICROCAPSULES

The present disclosure relates to the manufacturing of specifically targeting microcapsules comprising agrochemicals. More specifically, the disclosure relates to specifically targeting microcapsules, to which targeting agents are covalently linked at a ratio from about 0.01 μg-to about 1 μg targeting agents per square centimeter of the surface of the microcapsule, such that the microcapsules are capable of binding the agrochemicals contained in the microcapsules to a surface, and to agrochemical compositions comprising such microcapsules. 1. A process for manufacturing specifically targeting microcapsules , the process comprising at least the steps of:a. emulsifying into a continuous aqueous phase, an organic phase in which a to be encapsulated agrochemical or combination of agrochemicals is dissolved or dispersed to form an emulsion of droplets of the organic phase in the continuous aqueous phase;b. causing an aqueous suspension of microcapsules with polymer walls having anchor groups at their surface to be formed; andc. covalently linking at least one targeting agent to the anchor groups at the microcapsule surface, at a ratio from about 0.01 μg to about 1 μg targeting agent per square cm microcapsule surface.2. The process according to claim 1 , wherein the process comprises the steps of:emulsifying into a continuous aqueous phase comprising a surfactant, an organic phase in which a to be encapsulated agrochemical or combination of agrochemicals together with polyfunctional monomers or prepolymers are dissolved or dispersed to form an emulsion of droplets of the organic phase in the continuous aqueous phase;adding to the emulsion a monomer- or prepolymer-reactant component containing anchor groups;causing polymerization of the monomers or prepolymers to form an aqueous suspension of microcapsules having anchor groups at their surface; andcovalently linking at least one targeting agent to the anchor groups at the microcapsule surface, at a ratio from about 0.01 μ ...

Amino acid sequences that modulate the interaction between cells of the immune system

The present invention relates to amino acid sequences that block the interaction between (a target on) an antigen presenting cell (APC) and (a target on) a T-cell. More particularly, the present invention relates to amino acid sequences that are directed against (as defined herein) a target on an APC (also referred to herein as “APC target”) or a target on a T-cell (also referred to herein as “T-cell target”). The invention further relates to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences.

AGROCHEMICAL COMPOSITIONS COMPRISING ANTIBODIES BINDING TO SPHINGOLIPIDS

The present invention relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one heavy chain variable domain of an antibody, which specifically binds to a sphingolipid of a plant pathogen. The invention further provides methods for protecting or treating a plant or a part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of applying directly or indirectly to a plant or to a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or a part of a plant against a infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, to heavy chain variable domains with a specific pesticidal activity comprised within an agrochemical formulation, to nucleic acids encoding such heavy chain variable domains and to plants comprising chimeric genes comprising such nucleic acids. 1. An agrochemical composition comprising at least one V , which specifically binds to a glucosylceramide of a plant pathogenic fungus.24.-. (canceled)5Alternaria, Ascochyta, Botrytis, Cercospora, Colletotrichum, Diplodia, Erysiphe, Fusarium, Leptosphaeria, Gaeumanomyces, Helminthosporium, Macrophomina, Nectria, Penicillium, Peronospora, Phoma, Phymatotrichum, Phytophthora, Plasmopara, Podosphaera, Puccinia, Pyrenophora, Pyricularia, Pythium, Rhizoctonia, Scerotium, Sclerotinia, Septoria, Thielaviopsis, Uncinula, Venturia, Verticillium, Magnaporthe, Blumeria, Mycosphaerella, Ustilago, Melampsora, Phakospora, Monilinia, Mucor, RhizopusAspergillus.. The agrochemical composition according to claim 1 , wherein the genus of said plant pathogenic fungus is selected from the group consisting of claim 1 , and6. The agrochemical composition of claim 5 , wherein the at least one Vis present in the composition in an amount effective to protect or treat ...

TRANSGENIC PLANT COMPRISING A POLYNUCLEOTIDE ENCODING A VARIABLE DOMAIN OF HEAVY-CHAIN ANTIBODY

This disclosure relates to a transgenic plant or plant tissue. In particular this disclosure relates to a transgenic plant or plant tissue or plant cell comprising at least one polynucleotide comprising at least one sequence encoding a variable domain of a heavy-chain antibody (VHH) specifically binding to a sphingolipid of a fungus. Advantageously, the expression of the polynucleotide in at least part of the transgenic plant or plant tissue or plant cell (i) protects at least part of the transgenic plant or plant tissue or plant cell from an infection with a plant pathogenic fungus, (ii) inhibits the growth of a plant pathogenic fungus on at least part of the transgenic plant or plant tissue or plant cell, or (iii) increases the resistance of at least part of the transgenic plant or plant tissue or plant cell against a plant pathogenic fungus. This disclosure also relates to a method for protecting at least part of a plant or plant tissue or plant cell from an infection with a plant pathogen, for inhibiting the growth of a plant pathogen on at least part of a plant or plant tissue or plant cell, or for increasing pathogen resistance of at least part of a plant or plant tissue or plant cell, comprising expressing in at least part of the plant or plant tissue or plant cell at least one polynucleotide encoding a VHH specifically binding to a pathogen. 1. A transgenic plant or plant tissue or plant cell comprising:at least one polynucleotide comprising at least one sequence encoding a variable domain of a heavy-chain antibody (VHH) that specifically binds to a sphingolipid of a fungus.2. The transgenic plant or plant tissue or plant cell according to claim 1 , wherein the expression of the polynucleotide in at least part of the transgenic plant or plant tissue or plant cell (i) protects at least part of the transgenic plant or plant tissue or plant cell from an infection with a plant pathogenic fungus claim 1 , (ii) inhibits the growth of a plant pathogenic fungus on ...

METHODS FOR TREATING PEMPHIGUS DISORDERS

Provided are methods for treating pemphigus using an FcRn antagonist such as efgartigimod. The methods of the invention provide a rapid onset of action to enable early disease control and maintenance of clinical remission, with or without a minimal dose of corticosteroids. 1. A method of treating pemphigus , comprising (a) newly diagnosed pemphigus,', '(b) relapsing pemphigus, or', '(c) refractory pemphigus, and, '(i) selecting a subject that has'}(ii) administering to a subject in need thereof an effective amount of a human neonatal Fc receptor (FcRn) antagonist, wherein the FcRn antagonist consists of a variant Fc region, wherein said variant Fc region consists of two Fc domains which form a homodimer, and wherein the amino acid sequence of each of the Fc domains is selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 3,thereby treating pemphigus in the subject.2. The method of claim 1 , whereby disease control or complete remission is achieved.3. (canceled)4. The method of claim 1 , wherein disease control or complete remission is achieved and/or maintained at a corticosteroid dose of ≤0.5 mg prednisone/kg/day or equivalent.57-. (canceled)8. The method of claim 1 , wherein disease control or complete remission is achieved without co-administration of a corticosteroid.912-. (canceled)13. The method of claim 1 , wherein the pemphigus comprises pemphigus vulgaris (PV) claim 1 , pemphigus foliaceus (PF) claim 1 , or both PV and PF.14. The method of claim 1 , wherein the subject has mild claim 1 , moderate claim 1 , or severe pemphigus as classified by the Pemphigus Disease Area Index (PDAI).15. The method of claim 1 , wherein the FcRn antagonist is administered once weekly or biweekly until disease control or complete remission is achieved.16. The method of claim 1 , wherein the FcRn antagonist is administered intravenously at a dose of 10 mg/kg to 30 mg/kg once weekly or every two weeks.17. (canceled)18. (canceled)19. The method of claim ...

AMINO ACID SEQUENCES THAT MODULATE THE INTERACTION BETWEEN CELLS OF THE IMMUNE SYSTEM

The present invention relates to amino acid sequences that block the interaction between (a target on) an antigen presenting cell (APC) and (a target on) a T-cell. More particularly, the present invention relates to amino acid sequences that are directed against (as defined herein) a target on an APC (also referred to herein as “APC target”) or a target on a T-cell (also referred to herein as “T-cell target”). The invention further relates to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. 1441-. (canceled)442. A polypeptide that binds PD-L1 , that essentially consists of 4 framework regions (FR1 to FR4 respectively) and 3 complementarity determining regions (CDR1 to CDR3 respectively) in which: a) the amino acid sequences of SEQ ID NOs: 358-363,', 'b) amino acid sequences that have at least 80% amino acid identity with at least one of the amino acid sequences of SEQ ID NOs: 358-363 and', 'c) amino acid sequences that have 3, 2 or 1 amino acid difference with at least one of the amino acid sequences of SEQ ID NOs: 358-363;', 'and/or, 'CDR1 is chosen from the group consisting of d) the amino acid sequences of SEQ ID NOs: 370-375,', 'e) amino acid sequences that have at least 80% amino acid identity with at least one of the amino acid sequences of SEQ ID NOs: 370-375, and', 'f) amino acid sequences that have 3, 2 or 1 amino acid difference with at least one of the amino acid sequences of SEQ ID NOs: 370-375;', 'and/or, 'CDR2 is chosen from the group consisting of g) the amino acid sequences of SEQ ID NOs: 382-387,', 'h) amino acid sequences that have at least 80% amino acid identity with at least one of the amino acid sequences of SEQ ID NOs: 382-387, and', 'i) amino acid sequences that have 3, 2 or 1 amino acid difference with at least one of the amino acid sequences of SEQ ID NOs: 382-387., 'CDR3 is chosen from the group consisting of443. A polypeptide according to ...

TRANSGENIC PLANT COMPRISING A POLYNUCLEOTIDE ENCODING A VARIABLE DOMAIN OF HEAVY-CHAIN ANTIBODY

This disclosure relates to a transgenic plant or plant tissue. In particular, this disclosure relates to a transgenic plant or plant tissue or plant cell comprising at least one polynucleotide comprising at least one sequence encoding a variable domain of a heavy-chain antibody (VHH) specifically binding to a sphingolipid of a fungus. Advantageously, the expression of the polynucleotide in at least part of the transgenic plant or plant tissue or plant cell (i) protects at least part of the transgenic plant or plant tissue or plant cell from an infection with a plant pathogenic fungus, (ii) inhibits the growth of a plant pathogenic fungus on at least part of the transgenic plant or plant tissue or plant cell, or (iii) increases the resistance of at least part of the transgenic plant or plant tissue or plant cell against a plant pathogenic fungus. This disclosure also relates to a method for protecting at least part of a plant or plant tissue or plant cell from an infection with a plant pathogen, for inhibiting the growth of a plant pathogen on at least part of a plant or plant tissue or plant cell, or for increasing pathogen resistance of at least part of a plant or plant tissue or plant cell, comprising expressing in at least part of the plant or plant tissue or plant cell at least one polynucleotide encoding a VHH specifically binding to a pathogen. 1. A transgenic plant or plant tissue or plant cell comprising:at least one polynucleotide comprising at least one sequence encoding a variable domain of a heavy-chain antibody (VHH) that specifically binds to a sphingolipid of a fungus.2. The transgenic plant or plant tissue or plant cell according to claim 1 , wherein the expression of the polynucleotide in at least part of the transgenic plant or plant tissue or plant cell (i) protects at least part of the transgenic plant or plant tissue or plant cell from an infection with a plant pathogenic fungus claim 1 , (ii) inhibits the growth of a plant pathogenic fungus on ...

AGROCHEMICAL COMPOSITIONS COMPRISING ANTIBODIES BINDING TO SPHINGOLIPIDS

This disclosure relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one polypeptide, which specifically binds to a pest. The disclosure further provides methods for protecting or treating a plant or a part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of applying directly or indirectly to a plant or to a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or a part of a plant against a infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, to polypeptides with a specific pesticidal activity comprised within an agrochemical formulation, to nucleic acids encoding such polypeptide and to plants comprising chimeric genes comprising such nucleic acids. 115-. (canceled)16. A polypeptide which comprises: (a) one or more of the combinations chosen from the group consisting of:a CDR1 region having SEQ ID NO: 85, a CDR2 region having has SEQ ID NO: 169, and a CDR3 region having SEQ ID NO: 253, and/ora CDR1 region having SEQ ID NO: 86, a CDR2 region having has SEQ ID NO: 170, and a CDR3 region having SEQ ID NO: 254, and/ora CDR1 region having SEQ ID NO: 87, a CDR2 region having has SEQ ID NO: 171, and a CDR3 region having SEQ ID NO: 255, and/ora CDR1 region having SEQ ID NO: 88, a CDR2 region having has SEQ ID NO: 172, and a CDR3 region having SEQ ID NO: 256, and/ora CDR1 region having SEQ ID NO: 89, a CDR2 region having has SEQ ID NO: 173, and a CDR3 region having SEQ ID NO: 257, and/ora CDR1 region having SEQ ID NO: 90, a CDR2 region having has SEQ ID NO: 174, and a CDR3 region having SEQ ID NO: 258, and/ora CDR1 region having SEQ ID NO: 91, a CDR2 region having has SEQ ID NO: 175, and a CDR3 region having SEQ ID NO: 259, and/ora CDR1 region having SEQ ID NO: 92, a CDR2 region having has ...

AMINO ACID SEQUENCES THAT MODULATE THE INTERACTION BETWEEN CELLS OF THE IMMUNE SYSTEM

The present invention relates to amino acid sequences that block the interaction between (a target on) an antigen presenting cell (APC) and (a target on) a T-cell. More particularly, the present invention relates to amino acid sequences that are directed against (as defined herein) a target on an APC (also referred to herein as “APC target”) or a target on a T-cell (also referred to herein as “T-cell target”). The invention further relates to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. 1441.-. (canceled)442. Amino acid sequence that modulates , inhibits , prevents and/or blocks the interaction between a target on an antigen presenting cell (APC) and CD28. This application is a continuation of U.S. patent application Ser. No. 12/448,260, filed Jun. 15, 2009, which is a national stage filing under 35 U.S.C. §371 of international application PCT/EP2007/011057, filed Dec. 17, 2007, which was published under PCT Article 21(2) in English, and claims the benefit under 35 U.S.C. §119(e) of U.S. provisional application Ser. No. 60/875,246, filed Dec. 15, 2006 and of U.S. provisional application Ser. No. 60/993,053, filed Sep. 7, 2007, the disclosures of which are incorporated by reference herein in their entireties.The present invention relates to amino acid sequences that block the interaction between (a target on) an antigen presenting cell (APC) and (a target on) a T-cell. More particularly, the present invention relates to amino acid sequences that are directed against (as defined herein) a target on an APC (also referred to herein as “APC target”) or a target on a T-cell (also referred to herein as “T-cell target”), in particular, against targets that belong to the B7:CD28 superfamily (such as B7-1, B7-2, B7RP-1, PD-L1, PD-L2, B7H-3 and B7x [“APC targets”] and their receptors CD28, CTLA-4, ICOS, PD-1, BTLA and TIM-3 [“T-cell targets”]). The invention further relates ...

TRANSGENIC PLANT COMPRISING A POLYNUCLEOTIDE ENCODING A VARIABLE DOMAIN OF HEAVY-CHAIN ANTIBODY

This disclosure relates to a transgenic plant or plant tissue. In particular, this disclosure relates to a transgenic plant or plant tissue or plant cell comprising at least one polynucleotide comprising at least one sequence encoding a variable domain of a heavy-chain antibody (VHH) specifically binding to a sphingolipid of a fungus. Advantageously, the expression of the polynucleotide in at least part of the transgenic plant or plant tissue or plant cell (i) protects at least part of the transgenic plant or plant tissue or plant cell from an infection with a plant pathogenic fungus, (ii) inhibits the growth of a plant pathogenic fungus on at least part of the transgenic plant or plant tissue or plant cell, or (iii) increases the resistance of at least part of the transgenic plant or plant tissue or plant cell against a plant pathogenic fungus. This disclosure also relates to a method for protecting at least part of a plant or plant tissue or plant cell from an infection with a plant pathogen, for inhibiting the growth of a plant pathogen on at least part of a plant or plant tissue or plant cell, or for increasing pathogen resistance of at least part of a plant or plant tissue or plant cell, comprising expressing in at least part of the plant or plant tissue or plant cell at least one polynucleotide encoding a VHH specifically binding to a pathogen.

AMINO ACID SEQUENCES THAT MODULATE THE INTERACTION BETWEEN CELLS OF THE IMMUNE SYSTEM

The present invention relates to amino acid sequences that block the interaction between (a target on) an antigen presenting cell (APC) and (a target on) a T-cell. More particularly, the present invention relates to amino acid sequences that are directed against (as defined herein) a target on an APC (also referred to herein as “APC target”) or a target on a T-cell (also referred to herein as “T-cell target”). The invention further relates to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. 1441-. (canceled)442. A polypeptide that binds PD-1 , that essentially consists of 4 framework regions (FR1 to FR4 respectively) and 3 complementarity determining regions (CDR1 to CDR3 respectively) in which: a) the amino acid sequences of SEQ ID NOs: 317-321;', 'b) amino acid sequences that have at least 80% amino acid identity with at least one of the amino acid sequences of SEQ ID NOs: 317-321;', 'c) amino acid sequences that have 3, 2 or 1 amino acid difference with at least one of the amino acid sequences of SEQ ID NOs: 317-321;', 'CDR2 is chosen from the group consisting of:', 'and/or'}, 'd) the amino acid sequences of SEQ ID NOs: 327-331;', 'e) amino acid sequences that have at least 80% amino acid identity with at least one of the amino acid sequences of SEQ ID NOs: 327-331;', 'f) amino acid sequences that have 3, 2 or 1 amino acid difference with at least one of the amino acid sequences of SEQ ID NOs: 327-331;', 'CDR3 is chosen from the group consisting of:', 'and/or'}, 'g) the amino acid sequences of SEQ ID NOs: 337-341;', 'h) amino acid sequences that have at least 80% amino acid identity with at least one of the amino acid sequences of SEQ ID NOs: 337-341;', 'i) amino acid sequences that have 3, 2 or 1 amino acid difference with at least one of the amino acid sequences of SEQ ID NOs: 337-341., 'CDR1 is chosen from the group consisting of443. A polypeptide according to ...

AMINO ACID SEQUENCES THAT MODULATE THE INTERACTION BETWEEN CELLS OF THE IMMUNE SYSTEM

The present invention relates to amino acid sequences that block the interaction between (a target on) an antigen presenting cell (APC) and (a target on) a T-cell. More particularly, the present invention relates to amino acid sequences that are directed against (as defined herein) a target on an APC (also referred to herein as “APC target”) or a target on a T-cell (also referred to herein as “T-cell target”). The invention further relates to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. 1441.-. (canceled)442. A polypeptide that binds CTLA4 , that essentially consists of 4 framework regions (FR1 to FR4 respectively) and 3 complementarity determining regions (CDR1 to CDR3 respectively) in which:CDR1 is chosen from the group consisting of:a) the amino acid sequences of SEQ ID NOs: 664-767;b) amino acid sequences that have at least 80% amino acid identity with at least one of the amino acid sequences of SEQ ID NOs: 664-767;c) amino acid sequences that have 3, 2 or 1 amino acid difference with at least one of the amino acid sequences of SEQ ID NOs: 664-676;and/orCDR2 is chosen from the group consisting of:d) the amino acid sequences of SEQ ID NOs: 872-975;e) amino acid sequences that have at least 80% amino acid identity with at least one of the amino acid sequences of SEQ ID NOs: 872-975;f) amino acid sequences that have 3, 2 or 1 amino acid difference with at least one of the amino acid sequences of SEQ ID NOs: 872-975;and/orCDR3 is chosen from the group consisting of:g) the amino acid sequences of SEQ ID NOs: 1080-1183;h) amino acid sequences that have at least 80% amino acid identity with at least one of the amino acid sequences of SEQ ID NOs: 1080-1183;i) amino acid sequences that have 3, 2 or 1 amino acid difference with at least one of the amino acid sequences of SEQ ID NOs: 1080-1183.443. A polypeptide according to claim 442 , that can specifically bind CTLA4 ...

AMINO ACID SEQUENCES DIRECTED AGAINST CELLULAR RECEPTORS FOR VIRUSES AND BACTERIA

The present invention relates to amino acid sequences that are directed against (as defined herein) human cellular receptors for viruses and/or bacteria such as e.g. Nanobodies specifically recognizing hCD4, hCXCR4, hCCR5, hTLR4, human alphaV integrin, human beta3 integrin, human beta1 integrin, human alpha2 integrin, hCD81, hSR-BI, hClaudin-1, hClaudin-6 and hClaudin-9, as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. Said amino acid sequences may be used to prevent human cell entry of HIV, HCV, adenoviruses, hantavirus, herpesvirus, echo-virus 1 and others. 125.-. (canceled)26. An isolated polypeptide comprising one or more VHH , humanized VHH or camelized VH that specifically bind CXCR4 and that inhibit HIV entry into a cell.27. An isolated polypeptide comprising one or more VHH , humanized VHH or camelized VH that specifically bind CXCR4 and that inhibit HIV entry into a cell , wherein at least one VHH , humanized VHH or camelized VH consist essentially of 4 framework regions (FR1 to FR4 , respectively) and 3 complementarity determining regions (CDR1 to CDR3 , respectively) , 'CDR1 is chosen from the group consisting of:', 'in which'}a) the amino acid sequences of SEQ ID NOs: 142 to 143;b) amino acid sequences that have at least 80% amino acid identity with at least one of the amino acid sequences of SEQ ID NOs: 142 to 143; and 'CDR2 is chosen from the group consisting of:', 'c) amino acid sequences that have 3, 2, or 1 amino acid difference with at least one of the amino acid sequences of SEQ ID NOs: 142 to 143; and'}d) the amino acid sequences of SEQ ID NOs: 174 to 175;e) amino acid sequences that have at least 80% amino acid identity with at least one of the amino acid sequences of SEQ ID NOs: 174 to 175; and 'CDR3 is chosen from the group consisting of:', 'f) amino acid sequences that have 3, 2, or 1 amino acid difference with at least one of the ...

Specific delivery of agrochemicals

Described is the specific delivery of agrochemicals to plants. More specifically, a targeting agent has at least one binding domain that specifically binds to a binding site on an intact living plant. Such binding domains include a peptide having 4 framework regions and 3 complementary determining regions, or fragment(s) thereof, wherein the binding domains bind or retain a carrier onto a plant. Described are binding domains that specifically bind trichomes, stomata, cuticle, lenticels, thorns, spines, root hairs, or wax layer. Further described are methods for delivering agrochemicals to a plant, for depositing agrochemicals on a plant, and for retaining the agrochemicals on a plant, using targeting agents comprising the binding domains, and to methods for protecting a plant against stress or controlling plant growth. Also, described are methods for manufacturing a specifically targeting agrochemical carrier.

AGROCHEMICAL COMPOSITIONS COMPRISING ANTIBODIES BINDING TO SPHINGOLIPIDS

This disclosure relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one polypeptide, which specifically binds to a pest. The disclosure further provides methods for protecting or treating a plant or a part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of applying directly or indirectly to a plant or to a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or a part of a plant against a infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, to polypeptides with a specific pesticidal activity comprised within an agrochemical formulation, to nucleic acids encoding such polypeptide and to plants comprising chimeric genes comprising such nucleic acids. 115.-. (canceled)16. A polypeptide comprising: (a) one or more of the combinations selected from the group consisting of:a CDR1 region having SEQ ID NO: 85, a CDR2 region having has SEQ ID NO: 169, and a CDR3 region having SEQ ID NO: 253;a CDR1 region having SEQ ID NO: 86, a CDR2 region having has SEQ ID NO: 170, and a CDR3 region having SEQ ID NO: 254;a CDR1 region having SEQ ID NO: 87, a CDR2 region having has SEQ ID NO: 171, and a CDR3 region having SEQ ID NO: 255;a CDR1 region having SEQ ID NO: 88, a CDR2 region having has SEQ ID NO: 172, and a CDR3 region having SEQ ID NO: 256;a CDR1 region having SEQ ID NO: 89, a CDR2 region having has SEQ ID NO: 173, and a CDR3 region having SEQ ID NO: 257;a CDR1 region having SEQ ID NO: 90, a CDR2 region having has SEQ ID NO: 174, and a CDR3 region having SEQ ID NO: 258,a CDR1 region having SEQ ID NO: 91, a CDR2 region having has SEQ ID NO: 175, and a CDR3 region having SEQ ID NO: 259;a CDR1 region having SEQ ID NO: 92, a CDR2 region having has SEQ ID NO: 176, and a CDR3 region having SEQ ID NO: ...

Insect binding antibodies

The present invention relates to a binding domain, preferably an antigen binding domain, more preferably an antigen binding domain that specifically binds a binding site on an insect. More specifically, it relates to antigen binding domains comprising an amino acid sequence that comprises 4 framework regions and 3 complementary determining regions, whereby said antigen binding domains are capable to bind specifically to an insect as a whole, preferably to a binding site on the insect surface. The invention relates further to the use of said binding domain to deliver a compound, preferably a biologically active agent to the insect, and to insecticidal compositions comprising said binding domain.

Agrochemical compositions comprising antibodies binding to sphingolipids

The present invention relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one heavy chain variable domain of an antibody, which specifically binds to a sphingolipid of a plant pathogen. The invention further provides methods for protecting or treating a plant or a part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of applying directly or indirectly to a plant or to a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or a part of a plant against a infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, to heavy chain variable domains with a specific pesticidal activity comprised within an agrochemical formulation, to nucleic acids encoding such heavy chain variable domains and to plants comprising chimeric genes comprising such nucleic acids.

Transgenic plant comprising a polynucleotide encoding a variable domain of heavy-chain antibody

The present invention relates to a transgenic plant or plant tissue. In particular, the present invention relates to a transgenic plant or plant tissue or plant cell comprising at least one polynucleotide comprising at least one sequence encoding a variable domain of a heavy-chain antibody (VHH) specifically binding to a sphingolipid of a fungus. Advantageously, the expression of the polynucleotide in at least part of the transgenic plant or plant tissue or plant cell (i) protects at least part of the transgenic plant or plant tissue or plant cell from an infection with a plant pathogenic fungus, (ii) inhibits the growth of a plant pathogenic fungus on at least part of the transgenic plant or plant tissue or plant cell, or (iii) increases the resistance of at least part of the transgenic plant or plant tissue or plant cell against a plant pathogenic fungus. The present invention also relates to a method for protecting at least part of a plant or plant tissue or plant cell from an infection with a plant pathogen, for inhibiting the growth of a plant pathogen on at least part of a plant or plant tissue or plant cell, or for increasing pathogen resistance of at least part of a plant or plant tissue or plant cell, comprising expressing in at least part of the plant or plant tissue or plant cell at least one polynucleotide encoding a VHH specifically binding to a pathogen.

Agrochemical compositions comprising polypeptides

The present invention relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one polypeptide, which specifically binds to a pest. The invention further provides methods for protecting or treating a plant or a part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of applying directly or indirectly to a plant or to a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or a part of a plant against a infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, to polypeptides with a specific pesticidal activity comprised within an agrochemical formulation, to nucleic acids encoding such polypeptide and to plants comprising chimeric genes comprising such nucleic acids.

Agrochemical compositions comprising antibodies binding to sphingolipids

The present invention relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one polypeptide, which specifically binds to a pest. The invention further provides methods for protecting or treating a plant or a part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of applying directly or indirectly to a plant or to a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or a part of a plant against a infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, to polypeptides with a specific pesticidal activity comprised within an agrochemical formulation, to nucleic acids encoding such polypeptide and to plants comprising chimeric genes comprising such nucleic acids.

Agrochemical compositions comprising antibodies binding to sphingolipids

The present invention relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one polypeptide, which specifically binds to a pest. The invention further provides methods for protecting or treating a plant or a part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of applying directly or indirectly to a plant or to a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or a part of a plant against a infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, to polypeptides with a specific pesticidal activity comprised within an agrochemical formulation, to nucleic acids encoding such polypeptide and to plants comprising chimeric genes comprising such nucleic acids.

Agrochemical compositions comprising antibodies binding to sphingolipids

This disclosure relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one polypeptide, which specifically binds to a pest. The disclosure further provides methods for protecting or treating a plant or a part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of applying directly or indirectly to a plant or to a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or a part of a plant against a infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, to polypeptides with a specific pesticidal activity comprised within an agrochemical formulation, to nucleic acids encoding such polypeptide and to plants comprising chimeric genes comprising such nucleic acids.

Agrochemical compositions comprising antibodies binding to sphingolipids

This disclosure relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one polypeptide, which specifically binds to a pest. The disclosure further provides methods for protecting or treating a plant or a part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of applying directly or indirectly to a plant or to a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or a part of a plant against a infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, to polypeptides with a specific pesticidal activity comprised within an agrochemical formulation, to nucleic acids encoding such polypeptide and to plants comprising chimeric genes comprising such nucleic acids.

Transgenic plants expressing a variable domain of a heavy chain antibody (VHH) that binds to a sphingolipid of a fungus

This disclosure relates to a transgenic plant or plant tissue or plant cell comprising at least one polynucleotide encoding a polypeptide comprising a variable domain of a heavy-chain antibody (VHH) specifically binding to a sphingolipid of a fungus. This disclosure further relates to a method for protecting at least part of a plant, plant tissue or plant cell from an infection with a plant pathogen, for inhibiting the growth of a plant pathogen on at least part of a plant, plant tissue or plant cell, or for increasing pathogen resistance of at least part of a plant, plant tissue or plant cell.

Peptides capable of binding to serum proteins and compounds, constructs and polypeptides comprising the same

The present invention relates to amino acid sequences that are capable of binding to serum proteins; to compounds, proteins, polypeptides, fusion proteins or constructs comprising or essentially consisting of such amino acid sequences; to nucleic acids that encode such amino acid sequences, compounds, proteins, polypeptides, fusion proteins or constructs; to compositions, and in particular pharmaceutical compositions, that comprise such amino acid sequences, compounds, proteins, polypeptides, fusion proteins or constructs; and to uses of such amino acid sequences, compounds, proteins, polypeptides, fusion proteins or constructs.

Methods for treating pemphigus disorders

Provided are methods for treating pemphigus using an FcRn antagonist such as efgartigimod. The methods of the invention provide a rapid onset of action to enable early disease control and maintenance of clinical remission, with or without a minimal dose of corticosteroids.

Agrochemical compositions comprising antibodies binding to sphingolipids

AMINO ACID SEQUENCES TARGETING HUMAN CD4 and CXCR4, CCR5, TLR4, ALPHAV INTEGRIN, BETA3-INTEGRIN,BETA1-INTEGRIN, HUMAN ALPHA2-INTEGRIN, CD81, SR-BI, CLAUDIN-1, CLAUDIN-6 AND/OR CLAUDIN-9, RESPECTIVELY, AND NEUTRALIZING VIRAL ENTRY

TRANSGENIC PLANT THAT INCLUDES A POLINUCLEOTIDE THAT CODIFIES A VARIABLE DOMAIN OF HEAVY CHAIN ANTIBODY

Reivindicación 1: Una planta o tejido vegetal o célula vegetal transgénico que comprende al menos un polinucleótido que comprende al menos una secuencia que codifica para un dominio variable de un anticuerpo de cadena pesada (VHH) que se une específicamente a un esfingolípido de un hongo, preferiblemente a una ceramida de un hongo, más preferiblemente a un glicoesfingolípido de un hongo, incluso más preferiblemente a un cerebrósido de un hongo, aún más preferiblemente a un glucocerebrósido de un hongo. Reivindicación 8: Partes cosechables y materiales de propagación de una planta o tejido vegetal o célula vegetal transgénico de acuerdo con una cualquiera de las reivindicaciones 1 a 7, que comprende al menos un polinucleótido como se lo define en una cualquiera de las reivindicaciones 1 a 7. Reivindicación 11: Un método para proteger al menos parte de una planta o tejido vegetal o célula vegetal de una infección con un patógeno de planta, para inhibir el crecimiento de un patógeno de planta en al menos parte de una planta o tejido vegetal o célula vegetal, y/o para incrementar la resistencia a patógenos de al menos parte de una planta o tejido vegetal o célula vegetal, que comprende expresar en al menos parte de la planta o tejido vegetal o célula vegetal al menos un polinucleótido que codifica un dominio variable de un anticuerpo de cadena pesada (VHH) que se une específicamente a un patógeno. Reivindicación 13: Uso de al menos un polinucleótido que codifica un dominio variable de un anticuerpo de cadena pesada (VHH) que se une específicamente a un patógeno, para proteger al menos parte de una planta o tejido vegetal o célula vegetal de una infección con un patógeno de planta, para inhibir el crecimiento de un patógeno de planta en al menos parte de una planta o tejido vegetal o célula vegetal, y/o para incrementar la resistencia a patógenos de al menos parte de una planta o tejido vegetal o célula vegetal, en donde el polinucleótido es expresado en al menos parte ...

Amino acid sequences directed against vascular endothelial growth factor and polypeptides comprising the same for the treatment of conditions and diseases characterized by excessive and/or pathological angiogenesis or neovascularization

The present invention relates to amino acid sequences that are directed against vascular endothelial growth factor (VEGF), as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. The amino acid sequences, compounds and constructs can be used for prophylactic, therapeutic or diagnostic purposes, such as for the treatment of conditions and diseases characterized by excessive and/or pathological angiogenesis or neovascularization.

Monovalent phage display of single variable domains

The present invention relates to methods of generating single variable domains of high affinity, to libraries or repertoires comprising said single variable domains on phages in a monovalent format and to plasmids, phagemids or phages designed to express said single variable domains in said libraries.

Amino acid sequences that modulate the interaction between cells of the immune system

The present invention relates to amino acid sequences that block the interaction between (a target on) an antigen presenting cell (APC) and (a target on) a T-cell. More particularly, the present invention relates to amino acid sequences that are directed against (as defined herein) a target on an APC (also referred to herein as “APC target”) or a target on a T-cell (also referred to herein as “T-cell target”). The invention further relates to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences.

Manufacturing of specifically targeting microcapsules

The present invention relates to the manufacturing of specifically targeting microcapsules comprising agrochemicals. More specifically, the invention relates to specifically targeting microcapsules, to which targeting agents are covalently linked at a ratio from about 0,01µg to about 1µg targeting agents per square centimeter of the surface of the microcapsule, such that the microcapsules are capable of binding the agrochemicals contained in the microcapsules to a surface, and to agrochemical compositions comprising such microcapsules.

Agrochemical compositions comprising antibodies binding to sphingolipids

Compositions for seed treatment

The present invention relates to a composition for seed treatment comprising a plant seed binding protein, preferably an antigen binding plant seed binding protein. In a preferred embodiment, the seed binding protein is binding a polysaccharide, preferably pectin. The invention relates further to the use of a plant seed binding protein to bind a plant enhancing agent to a plant seed, and to a method for treating plant seeds.

COMPOSITION COMPRISING AT LEAST ONE POLYPEPTIDE THAT SPECIFICALLY BINDS TO A GLYCOSYLCERAMIDE FROM A FUNGUS PEST

COMPOSIÇÕES AGROQUÍMICAS COMPREENDENDO ANTICORPOS DE LIGAÇÃO A ESFINGOLIPÍDIO. A presente invenção refere-se a composições de controle agroquímicas e biológicas para comba ter pragas, mais especificamente pragas de plantas, compreendendo pelo menos um polipeptídeo, que se liga especificamente a uma praga . A invenção refere-se ainda a métodos para a proteção ou tratamento de uma planta ou parte de uma planta de uma infecção ou outra interação biológica com um patógeno de planta, pelo menos, compreendendo a etapa de aplicar direta ou indiretamente a uma planta ou a uma parte de uma planta, uma composição agroquímica, sob condições eficazes para proteger ou tratar uma planta ou parte de uma planta contra uma infecção ou interação biológica com um patógeno da planta. São ainda proporcionados métodos para a produção de tais composições e formulações agroquímicas, com polipeptídeos com uma atividade pesticida específica compreendida dentro de uma formulação agroquímica, a ácidos nucleicos que codificam polipeptídeo , tais como a as plantas que compreendem os genes quiméricos compreendendo tais ácidos nucleico. AGROCHEMICAL COMPOSITIONS COMPRISING SPHINGOLIPID BINDING ANTIBODIES. The present invention relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one polypeptide, which specifically binds to a pest. The invention further relates to methods for protecting or treating a plant or part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of directly or indirectly applying to a plant or a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or part of a plant against an infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, with polypeptides having a specific pesticidal activity comprised ...

Agrochemical compositions comprising antibodies that bind to sphingolipids

Amino acid sequences directed against vascular endothelial growth factor and polypeptides comprising the same for the treatment of conditions and diseases characterized by excessive and/or pathological angiogenesis or neovascularization

The present invention relates to amino acid sequences that are directed against vascular endothelial growth factor (VEGF), as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. The amino acid sequences, compounds and constructs can be used for prophylactic, therapeutic or diagnostic purposes, such as for the treatment of conditions and diseases characterized by excessive and/or pathological angiogenesis or neovascularization.

Amino acid sequences directed against cellular receptors for viruses and bacteria

The present invention relates to amino acid sequences that are directed against (as defined herein) human cellular receptors for viruses and/or bacteria such as e.g. NANOBODIES specifically recognizing hCD4, hCXCR4, hCCR5, hTLR4, human alphaV integrin, human beta3 integrin, human beta1 integrin, human alpha2 integrin, hCD81, hSR-BI, hClaudin-1, hClaudin-6 and hClaudin-9, as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. The amino acid sequences may be used to prevent human cell entry of HIV, HCV, adenoviruses, hantavirus, herpesvirus, echo-virus 1 and others.

Chitinous polysaccharide antigen binding proteins

The present invention relates to an antigen binding protein, preferably comprising an amino acid sequence that comprises 4 framework regions and 3 complementary determining regions, wherein said antigen binding protein is capable of binding a chitinous polysaccharide, and uses thereof.

Agrochemical compositions comprising antibodies binding to sphingolipids

Compositions for seed treatment

The present invention relates to a composition for seed treatment comprising a plant seed binding protein, preferably an antigen binding plant seed binding protein. In a preferred embodiment, the seed binding protein is binding a polysaccharide, preferably pectin. The invention relates further to the use of a plant seed binding protein to bind a plant enhancing agent to a plant seed, and to a method for treating plant seeds.

Peptides capable of binding to serum proteins

The present invention relates to amino acid sequences that are capable of binding to serum proteins; to compounds, proteins, polypeptides, fusion proteins or constructs comprising or essentially consisting of such amino acid sequences; to nucleic acids that encode such amino acid sequences, compounds, proteins, polypeptides, fusion proteins or constructs; to compositions, and in particular pharmaceutical compositions, that comprise such amino acid sequences, compounds, proteins, polypeptides, fusion proteins or constructs; and to uses of such amino acid sequences, compounds, proteins, polypeptides, fusion proteins or constructs.

Transgenic plant comprising a polynucleotide encoding a variable domain of heavy-chain antibody

The present invention relates to a transgenic plant or plant tissue. In particular, the present invention relates to a transgenic plant or plant tissue or plant cell comprising at least one polynucleotide comprising at least one sequence encoding a variable domain of a heavy-chain antibody (VHH) specifically binding to a sphingolipid of a fungus. Advantageously, the expression of the polynucleotide in at least part of the transgenic plant or plant tissue or plant cell (i) protects at least part of the transgenic plant or plant tissue or plant cell from an infection with a plant pathogenic fungus, (ii) inhibits the growth of a plant pathogenic fungus on at least part of the transgenic plant or plant tissue or plant cell, or (iii) increases the resistance of at least part of the transgenic plant or plant tissue or plant cell against a plant pathogenic fungus. The present invention also relates to a method for protecting at least part of a plant or plant tissue or plant cell from an infection with a plant pathogen, for inhibiting the growth of a plant pathogen on at least part of a plant or plant tissue or plant cell, or for increasing pathogen resistance of at least part of a plant or plant tissue or plant cell, comprising expressing in at least part of the plant or plant tissue or plant cell at least one polynucleotide encoding a VHH specifically binding to a pathogen.

Insect binding antibodies

The present invention relates to a binding domain, preferably an antigen binding domain, more preferably an antigen binding domain that specifically binds a binding site on an insect. More specifically, it relates to antigen binding domains comprising an amino acid sequence that comprises 4 framework regions and 3 complementary determining regions, whereby said antigen binding domains are capable to bind specifically to an insect as a whole, preferably to a binding site on the insect surface. The invention relates further to the use of said binding domain to deliver a compound, preferably a biologically active agent to the insect, and to insecticidal compositions comprising said binding domain.

Transgenic plant comprising a polynucleotide encoding a variable domain of heavy-chain antibody

The present invention relates to a transgenic plant or plant tissue. In particular, the present invention relates to a transgenic plant or plant tissue or plant cell comprising at least one polynucleotide comprising at least one sequence encoding a variable domain of a heavy-chain antibody (VHH) specifically binding to a sphingolipid of a fungus. Advantageously, the expression of the polynucleotide in at least part of the transgenic plant or plant tissue or plant cell (i) protects at least part of the transgenic plant or plant tissue or plant cell from an infection with a plant pathogenic fungus, (ii) inhibits the growth of a plant pathogenic fungus on at least part of the transgenic plant or plant tissue or plant cell, or (iii) increases the resistance of at least part of the transgenic plant or plant tissue or plant cell against a plant pathogenic fungus. The present invention also relates to a method for protecting at least part of a plant or plant tissue or plant cell from an infection with a plant pathogen, for inhibiting the growth of a plant pathogen on at least part of a plant or plant tissue or plant cell, or for increasing pathogen resistance of at least part of a plant or plant tissue or plant cell, comprising expressing in at least part of the plant or plant tissue or plant cell at least one polynucleotide encoding a VHH specifically binding to a pathogen.

Agrochemical compositions comprising antibodies binding to sphingolipids

H:\Interwoven\NRPortbl\DCC\FMT432572_1.docx-14/07/2020 ABSTRACT The present invention relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one polypeptide, which specifically binds to a pest. The invention further provides methods for protecting or treating a plant or a part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of applying directly or indirectly to a plant or to a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or a part of a plant against a infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, to polypeptides with a specific pesticidal activity comprised within an agrochemical formulation, to nucleic acids encoding such polypeptide and to plants comprising chimeric genes comprising such nucleic acids.

Chitinous polysaccharide antigen binding proteins

Amino acid sequences that modulate the interaction between cells of the immune system

Agrochemical compositions comprising antibodies binding to sphingolipids

The present invention relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one polypeptide, which specifically binds to a pest. The invention further provides methods for protecting or treating a plant or a part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of applying directly or indirectly to a plant or to a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or a part of a plant against a infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, to polypeptides with a specific pesticidal activity comprised within an agrochemical formulation, to nucleic acids encoding such polypeptide and to plants comprising chimeric genes comprising such nucleic acids.

Agrochemical compositions comprising antibodies binding to sphingolipids

The present invention relates to agrochemical and biological control compositions for combating pests, more specifically plant pests, comprising at least one heavy chain variable domain of an antibody, which specifically binds to a sphingolipid of a plant pathogen. The invention further provides methods for protecting or treating a plant or a part of a plant from an infection or other biological interaction with a plant pathogen, at least comprising the step of applying directly or indirectly to a plant or to a part of a plant, an agrochemical composition, under conditions effective to protect or treat a plant or a part of a plant against a infection or biological interaction with a plant pathogen. Further provided are methods for producing such agrochemical compositions and formulations, to heavy chain variable domains with a specific pesticidal activity comprised within an agrochemical formulation, to nucleic acids encoding such heavy chain variable domains and to plants comprising chimeric genes comprising such nucleic acids.

Specific delivery of agrochemicals

The present invention relates to specific delivery of agrochemicals to plants. More specifically, it relates to a composition, essentially consisting of a targeting agent comprising at least one binding domain that specifically binds to a binding site on an intact living plant and an agrochemical or a combination of agrochemicals. The present invention relates further to a binding domain that specifically binds said binding site on an intact living plant. More specifically, it relates to binding domains comprising an amino acid sequence that comprises 4 framework regions and 3 complementary determining regions, or any suitable fragment thereof, whereby said binding domains are capable to bind or retain a carrier onto a plant. In one preferred embodiment, the invention relates to binding domains which specifically bind trichomes, stomata, cuticle, lenticels, thorns, spines, root hairs, or wax layer. The invention relates further to a method for delivery of agrochemicals to a plant, for improving the deposition of agrochemicals on a plant,and for retaining said agrochemicals on a plant, using targeting agents comprising said binding domains, and to a method for protecting a plant against biotic or abiotic stress or controlling plant growth using the same. Also, the invention relates to a method for manufacturing a specifically targeting agrochemical carrier.

Methods for treating patients with an autoantibody-mediated disease

Provided herein are methods of treating an autoantibody-mediated disease in a subject, methods of monitoring treatment of an autoantibody-mediated disease in a subject, based on the frequency of B cells in the subject.

Amino acid sequences targeting human cd4, cxcr4, ccr5, tlr4, alphav integrin, beta3-integrin, beta1-integrin, human alpha2-integrin, cd81, sr-bi, claudin-1, claudin-6 and claudin-9, respectively, and neutralizing viral entry

The present invention relates to amino acid sequences that are directed against (as defined herein) human cellular receptors for viruses and/or bacteria such as e.g. Nanobodies specifically recognizing hCD4, hCXCR4, hCCR5. hTLR4, human alphaV integrin, human beta3 integrin, human beta1 integrin, human alpha2 integrin, hCD81, hSR-B1, hClaudin-1, hClaudin-6 and hClaudin-9, as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. Said amino acid sequences may be used to prevent human cell entry of HIV, HCV, adenoviruses, hantavirus, herpesvirus, echo-virus 1 and others.

Methods for treating bullous pemphigoid using fcrn antagonists

Provided herein are methods of treating bullous pemphigoid using an effective amount of a human neonatal Fc receptor (FcRn) antagonist. FcRn antagonists for use in the treatment of bullous pemphigoid and for use in the manufacture of a medicament for the treatment of bullous pemphigoid are also provided herein.

Subcutaneous unit dosage forms

Provided herein are unit dosage forms of a biologic that are determined based on a modeling approach, which matches a pharmacodynamic (PD) value of the SC dose with that of a known reference IV dose, while a pharmacokinetic (PK) value of the SC dose is less than that of the IV dose.

Métodos para tratar trastornos de pénfigo.

Se proporcionan métodos para tratar el pénfigo mediante el uso de un antagonista de FcRn tal como efgartigimod. Los métodos de la invención proporcionan un inicio rápido de la acción para permitir el control temprano de la enfermedad y el mantenimiento de la remisión clínica, con o sin una dosis mínima de corticoesteroides.

Subcutaneous unit dosage forms

Provided herein are unit dosage forms of a biologic that are determined based on a modeling approach, which matches a pharmacodynamic (PD) value of the SC dose with that of a known reference IV dose, while a pharmacokinetic (PK) value of the SC dose is less than that of the IV dose.

Subcutaneous unit dosage forms

Transgenic plant comprising a polynucleotide encoding a variable domain of heavy-chain antibody

Amino acid sequences that modulate the interaction between cells of the immune system

The present invention relates to amino acid sequences that block the interaction between (a target on) an antigen presenting cell (APC) and (a target on) a T-cell. More particularly, the present invention relates to amino acid sequences that are directed against (as defined herein) a target on an APC (also referred to herein as “APC target”) or a target on a T-cell (also referred to herein as “T-cell target”). The invention further relates to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences.

Methods for treating primary membranous nephropathy using fcrn antagonists

Provided herein are methods of treating primary membranous nephropathy using an effective amount of a human neonatal Fc receptor (FcRn) antagonist. FcRn antagonists for use in the treatment of primary membranous nephropathy and for use in the manufacture of a medicament for the treatment of primary membranous nephropathy are also provided herein.

Methods for treating lupus nephritis using fcrn antagonists

Provided herein are methods of treating lupus nephritis using an effective amount of a human neonatal Fc receptor (FcRn) antagonist. FcRn antagonists for use in the treatment of lupus nephritis and for use in the manufacture of a medicament for the treatment of lupus nephritis are also provided herein.

Transgenic plant comprising a polynucleotide encoding a variable domain of heavy-chain antibody

Amino acid sequences that modulate the interaction between cells of the immune system

The present invention relates to amino acid sequences that block the interaction between (a target on) an antigen presenting cell (APC) and (a target on) a T-cell. More particularly, the present invention relates to amino acid sequences that are directed against (as defined herein) a target on an APC (also referred to herein as “APC target”) or a target on a T-cell (also referred to herein as “T-cell target”). The invention further relates to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences.

Subcutaneous unit dosage forms

Provided herein are unit dosage forms of a biologic that are determined based on a modeling approach, which matches a pharmacodynamic (PD) value of the SC dose with that of a known reference IV dose, while a pharmacokinetic (PK) value of the SC dose is less than that of the IV dose.

Administración específica de productos agroquímicos

Un agente de direccionamiento, que comprende al menos un dominio de unión capaz de unirse a al menos un sitio de unión sobre una superficie vegetal, en donde dicho dominio de unión es un VHH, o cualquier fragmento adecuado del mismo, en donde el agente de direccionamiento está acoplado a al menos un producto agroquímico, o a un vehículo que comprende o está unido a al menos un producto agroquímico o una combinación de productos agroquímicos, en donde el agente de direccionamiento es capaz de retener dicho producto agroquímico o vehículo sobre dicha superficie vegetal.

Métodos para tratar a pacientes con una enfermedad mediada por autoanticuerpos

En la presente descripción se proporcionan métodos para tratar una enfermedad mediada por autoanticuerpos en un sujeto, métodos para monitorear el tratamiento de una enfermedad mediada por autoanticuerpos en un sujeto, en función de la frecuencia de linfocitos B en el sujeto. Reivindicación 1: Un método para monitorear la eficacia del tratamiento de una enfermedad mediada por autoanticuerpos en un sujeto después del tratamiento con un primer antagonista del FcRn, el método comprende: a) medir in vitro la frecuencia de linfocitos B en una muestra de sangre tomada del sujeto; y b) comparar la frecuencia de linfocitos B con un valor de referencia asociado con la enfermedad mediada por autoanticuerpos en el sujeto, en donde el tratamiento no es eficaz si la frecuencia de los linfocitos B en la muestra es mayor o igual al valor de referencia, y en donde el tratamiento es eficaz si la frecuencia de los linfocitos B es menor que el valor de referencia. Reivindicación 2: Un método para tratar una enfermedad mediada por autoanticuerpos en un sujeto que ha recibido un primer antagonista del FcRn y está recibiendo un régimen de dosis de corticoesteroides, el método comprende: a) administrar al sujeto una cantidad terapéuticamente eficaz de un segundo antagonista del FcRn; b) medir in vitro la frecuencia de linfocitos B en una muestra de sangre tomada del sujeto; y c) comparar la frecuencia de linfocitos B con un valor de referencia asociado con la enfermedad mediada por autoanticuerpos en el sujeto, en donde el régimen de dosis de corticoesteroides se mantiene si la frecuencia de linfocitos B en la muestra es mayor o igual que el valor de referencia, o en donde el régimen de dosis de corticoesteroides se reduce gradualmente si la frecuencia de linfocitos B es menor que el valor de referencia. Reivindicación 3: Un segundo antagonista del FcRn para usar en un método para tratar una enfermedad mediada por autoanticuerpos en un sujeto que ha recibido un primer antagonista del FcRn ...

Methods for treating patients with an autoantibody-mediated disease

Provided herein are methods of treating an autoantibody-mediated disease in a subject, methods of monitoring treatment of an autoantibody-mediated disease in a subject, based on the frequency of B cells in the subject.

Agrokimyasalların spesifik aktarımı.

AGROKİMYASALLARIN SPESİFİK AKTARIMI Buluş, agrokimyasalların bitkilere spesifik aktarımı ile ilgilidir. Daha özel olarak, intakt canlı bitki ve agrokimyasal veya agrokimyasalların kombinasyonu üzerinde bağlama yerini spesifik olarak bağlayabilen en az bir bağlama alanı içeren temelde bir hedefleme ajanından oluşan bir bileşim ile ilgilidir. Buluş, ayrıca intakt canlı bitki üzerinde söz konusu bağlama yerine spesifik olarak bağlanan bir bağlama alanı ile ilgildir. Daha özel olarak bu 4 çerçeve bölgesi ve 3 tamamlayıcılık belirleme bölgesi veya bunun herhangi uygun bir parçasını içeren bir aminoasit dizisi içeren bağlama alanları ile ilgilidir, böylece söz konusu bağlama alanları bitki üzerindeki bir taşıyıcıyı bağlayabilir veya sürdürebilir. Tercih edilen bir düzenlemede buluş, trikoma, stomaya, kovucuklara, dikenlere, kılçıklara, kök tüylerine veya mumsu tabakaya spesifik olarak bağlanabilen bağlama alanları ile ilgilidir. Buluş ayrıca, bu bağlama alanlarını içeren hedefleme ajanları kullanılarak bahsedilen agrokimyasalları bir bitki üzerinde tutmaya yönelik, bir bitki üzerinde agrokimyasalların biriktirilmesini artırmaya yönelik, bir bitkiye agrokimyasalların dağıtılmasına yönelik bir yönteme ve bunu kullanarak bitki büyümesini kontrol etmek veya abiyotik strese veya biyotik strese karşı bir bitkinin korunmasına yönelik bir yönteme ilişkindir. Ayrıca buluş, özel olarak bir hedefleyici agrokimyasal taşıyıcıyı üretmeye yönelik bir yönteme ilişkindir.

Specific delivery of agrochemicals

The present invention relates to specific delivery of agrochemicals to plants. More specifically, it relates to a composition, essentially consisting of a targeting agent comprising at least one binding domain that specifically binds to a binding site on an intact living plant and an agrochemical or a combination of agrochemicals. The present invention relates further to a binding domain that specifically binds said binding site on an intact living plant. More specifically, it relates to binding domains comprising an amino acid sequence that comprises 4 framework regions and 3 complementary determining regions, or any suitable fragment thereof, whereby said binding domains are capable to bind or retain a carrier onto a plant. In one preferred embodiment, the invention relates to binding domains which specifically bind trichomes, stomata, cuticle, lenticels, thorns, spines, root hairs, or wax layer. The invention relates further to a method for delivery of agrochemicals to a plant, for improving the deposition of agrochemicals on a plant,and for retaining said agrochemicals on a plant, using targeting agents comprising said binding domains, and to a method for protecting a plant against biotic or abiotic stress or controlling plant growth using the same. Also, the invention relates to a method for manufacturing a specifically targeting agrochemical carrier.

Formas de dosificación unitaria subcutánea.

En la presente descripción se proporcionan formas de dosificación unitaria de un producto biológico que se determinan con base en un enfoque de modelado, que coincide con un valor farmacodinámico (PD) de la dosis SC con el de una dosis IV de referencia conocida, mientras que un valor farmacocinético (PK) de la dosis SC es menor que el de la dosis IV.

Métodos para trata el penfigoide ampolloso mediante el uso de antagonistas del fcrn

Se proporcionan en la presente descripción métodos para tratar el penfigoide ampolloso mediante el uso de una cantidad efectiva de un antagonista del receptor de Fc neonatal (FcRn) humano. También se proporcionan en la presente descripción antagonistas del FcRn para su uso en el tratamiento del penfigoide ampolloso y para su uso en la fabricación de un medicamento para el tratamiento del penfigoide ampolloso. Reivindicación 1: Un método para tratar el penfigoide ampolloso (PA) en un sujeto que lo necesita, el método comprende administrar al sujeto una cantidad efectiva de un antagonista del receptor de Fc neonatal (FcRn) humano. Reivindicación 77: Un antagonista del FcRn para su uso en el tratamiento del PA, en donde el tratamiento se realiza de acuerdo con el método de una cualquiera de las reivindicaciones anteriores. Reivindicación 78: Un antagonista del FcRn para su uso en la fabricación de un medicamento para el tratamiento del PA, en donde el tratamiento se realiza de acuerdo con el método de una cualquiera de las reivindicaciones anteriores. Reivindicación 79: Un método para monitorear la eficacia del tratamiento del PA en un sujeto después del tratamiento con un primer antagonista del FcRn, el método comprende: a) medir in vitro un nivel en suero de un anticuerpo anti-BP180 y/o un anticuerpo anti-BP230 en una muestra de sangre tomada del sujeto; y b) comparar el nivel en suero del anticuerpo anti-BP180 y/o el anticuerpo anti-BP230 con un valor de referencia asociado con el PA en el sujeto, en donde el tratamiento no es efectivo si el nivel en suero del anticuerpo anti-BP180 y/o el anticuerpo anti-BP230 en la muestra es mayor o igual que el valor de referencia, y en donde el tratamiento es efectivo si el nivel en suero del anticuerpo anti-BP180 y/o el anticuerpo anti-BP230 es menor que el valor de referencia.

Methods for treating patients with an autoantibody-mediated disease

Provided herein are methods of treating an autoantibody-mediated disease in a subject, methods of monitoring treatment of an autoantibody-mediated disease in a subject, based on the frequency of B cells in the subject.

Methods for treating patients with an autoantibody-mediated disease

Antagonists of human neonatal fc receptor (fcrn) for treating pemphigus disorders

Žmogaus neonatalinių fc receptorių (fcrn) antagonistai, skirti pūslinių ligų gydymui

Vastasyntyneen ihmisen Fc-reseptorin (FcRn) antagonisteja pemfigus-häiriöiden hoitoon

Antagonister af human neonatal Fc-receptor (FcRn) til behandling af pemphigus-sygdomme

Methods for treating pemphigus disorders

Provided are methods for treating pemphigus using an FcRn antagonist such as efgartigimod. The methods of the invention provide a rapid onset of action to enable early disease control and maintenance of clinical remission, with or without a minimal dose of corticosteroids

Amino acid sequences directed against cellular receptors for viruses and bacteria

The present invention relates to amino acid sequences that are directed against (as defined herein) human cellular receptors for viruses and/or bacteria such as e.g. Nanobodies specifically recognizing hCD4, hCXCR4, hCCR5, hTLR4, human alphaV integrin, human beta3 integrin, human beta1 integrin, human alpha2 integrin, hCD81, hSR-BI, hClaudin-1, hClaudin-6 and hClaudin-9, as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. Amino acid sequences of the present invention may be used to prevent human cell entry of HIV, HCV, adenoviruses, hantavirus, herpesvirus, echo-virus 1 and others.

Chitinous polysaccharide antigen-binding proteins

Described is an antigen-binding protein, preferably comprising an amino acid sequence that comprises four framework regions and three complementarity-determining regions, wherein the antigen-binding protein is capable of binding a chitinous polysaccharide, and uses thereof.