26-06-2014 дата публикации
Номер: US20140179553A1
Принадлежит:
Two genes required for this mercury methylation have been identified in bacteria and archaea. These genes are the hgcA gene and hgcB, a corrinoid protein that facilitates methyl group transfer to Hg, and a corrinoid protein-associated ferredoxin with two [4Fe-4S] binding motifs involved in generating cob(I)almin, respectively. The invention provides nucleic acid probes and primers for detecting methylmercury and or for assessing mercury methylation potential in environmental, clinical and other samples. The invention also provides antibodies against these proteins, antibodies against these proteins, methods of using the antibodies and methods of biocatalysis. 16-. (canceled)7. One or more sets of forward and reverse PCR primers capable of amplifying from at least a 25 bp fragment to all of a microbial hgcA gene , a microbial hgcB gene or both , wherein gene includes upstream and downstream regions associated with expression of the hgcA or hgcB coding sequence.8. The primers of claim 7 , wherein said microbial hgcA gene or said microbial hgcB gene is from a microorganism listed in Table 1.921-. (canceled)22. A method to detect microorganisms capable of mercury methylation in a sample which comprises(a) preparing nucleic acids from a sample; and(b) detecting the presence of an hgcA gene, an hgcB gene or both in said sample by hybridization with one or more nucleic acid probes or primers specific for hgcA, hgcB or both.23. The method of claim 22 , wherein said detection is by a microarray-based assay claim 22 , PCR claim 22 , in situ hybridization claim 22 , southern blot claim 22 , or northern blot.24. The method of claim 22 , wherein said one or more probes or primers are selected from the group consisting of(a) an isolated nucleic acid which comprises a length of contiguous hgcA or hgcB nucleotides sufficient to specifically detect nucleic acids encoding hgcA or hgcB;{'figref': [{'@idref': 'DRAWINGS', 'FIG. 5'}, {'@idref': 'DRAWINGS', 'FIG. 5'}], '(b) said ...
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