14-04-2023 дата публикации
Номер: CN115961013A
Принадлежит:
The invention discloses screening of a schistosoma japonicum W chromosome specific gene and application of the schistosoma japonicum W chromosome specific gene in cercaria sex identification, particularly relates to a duplex PCR (polymerase chain reaction) method adopting a specific primer containing the schistosoma japonicum W chromosome specific gene and a schistosoma japonicum reference gene PSMD4 primer, and belongs to the technical field of biology. The sex of the cercaria of the schistosoma japonicum katsurada is rapidly identified by using a schistosoma japonicum katsurada W chromosome specific gene primer and a PSMD4 housekeeping gene primer and adopting a dual PCR method, the whole process only needs 2 hours, and compared with a traditional animal experiment identification method, the identification time is greatly shortened, and the standardization and homogenization of a schistosoma japonicum katsurada disease animal model are improved; meanwhile, cercaria DNA does not need to ...
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