17-10-2019 дата публикации
Номер: US20190317050A1
Автор:
BAO Dongqin,
Hong Bin,
HUANG Fushen,
HUANG Jingyuan,
PAN Weisong,
SHEN Haibo,
WU Kebin,
XIONG Lun,
Zhao Jia,
ZHOU Tiean,
Zhou Zhen
Принадлежит:
A real time and quantitative method of measuring traction force of living cells include the following procedures. Place AT-cut and BT-cut quartz crystals of the same frequency, surface morphology and/or modified with the same cell adhesion molecules in petri dishes or detection cells; add the cells to the petri dishes or detection cells, the cell traction force at arbitrary time t during adhesion of the cells or under different internal/external environmental stimulations is estimated by the following equation: ΔS=(K−K)[tΔf/fr−tqΔf/fr]. The method can be used to track the dynamic changes of cells generated force during the adhesion of cells and under different internal/external environmental stimulations, such as the effects of drugs. The drugs can be added before or after the adhesion of the cells. This method is suitable for all adherent cells, including primary cells and passage cells. 1. A real-time and quantitative measurement method for cell traction force , comprising the following steps:(1) placing an AT-cut quartz crystal and a BT-cut quartz crystal in culture dishes or detection cells, wherein the AT-cut quartz crystal having the same frequency, surface morphology and/or modified surface adhesion molecules as those of the BT-cut quartz crystal; and{'sub': 't', 'claim-text': [{'br': None, 'i': S', 'K', '−K', 't', 'Δf', '/fr', '−tq', 'Δf', '/fr, 'sub': t', 'AT', 'BT', 'q', 't', 't, 'sup': −1', 'AT', 'AT', 'AT', 'BT', 'BT', 'BT, 'Δ=()[]\u2003\u2003(1)'}, {'sub': AT', 'BT', 't', 't, 'sup': −12', '2', '−1', '−12', '2', '−1', 'AT', 'BT', 'AT', 'BT', 'BT', 'BT, 'wherein K=2.75×10cmdynand K=−2.65×10cmdynare stress coefficients of the AT-cut quartz crystal and the BT-cut quartz crystal respectively; fris the resonant frequency of the AT-cut quartz crystal, fris the resonant frequency of the BT-cut quartz crystal, tqis the thickness of the AT-cut quartz crystal, tqis the thickness of the BT-cut quartz crystal, and all of which are constants; Δfand Δfare the ...
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