30-09-2021 дата публикации
Номер: US20210301000A1
Принадлежит:
Provided is an isolated binding protein including an antigen binding domain binding to an NS1 protein. The binding protein can identify and bind to the NS1 protein so as to detect dengue virus. 1. An isolated binding protein comprising an antigen binding domain , wherein the antigen binding domain comprises at least one complementarity determining region selected from the following amino acid sequences ,{'sub': 'D', 'sup': '−8', 'or has at least 80% sequence identity with the complementarity determining region of the following amino acid sequences and has an affinity of K≤6.55×10mol/L to a NS1 protein;'}the complementarity determining region CDR-VH1 is G-Y-X1-F-T-X2-Y-W-1-G, wherein,X1 is S or T, and X2 is D or E;the complementarity determining region CDR-VH2 is D-M-X1-P-G-D-X2-Y-1-N-Y-X3-E-K-F-K-G, wherein,X1 is F or V, X2 is V, L or I, and X3 is Q or N;the complementarity determining region CDR-VH3 is T-N-F-X1-T-X2-G-G-X3-D-Y, wherein,X1 is I or L, X2 is L or V, and X31 is V, L or I;the complementarity determining region CDR-VL1 is K-S-S-X1-S-L-L-X2-S-D-G-X3-T-Y-L-N, wherein,X1 is Q or N, X2 is E or D, and X3 is R or K;the complementarity determining region CDR-VL2 is L-V-X1-K-X2-D-S, wherein,X1 is S or T, and X2 is V, I or L;the complementarity determining region CDR-VL3 is W-X1-G-T-H-F-X2-H-T, wherein,X1 is Q, Y or W, and X2 is A or P.3. The binding protein according to claim 1 , wherein the binding protein comprises at least 3 CDRs; or the binding protein comprises at least 6 CDRs.4. The binding protein according to claim 1 , wherein the binding protein is one of a nanobody claim 1 , F(ab′)2 claim 1 , Fab′ claim 1 , Fab claim 1 , Fv claim 1 , scFv claim 1 , a bispecific antibody claim 1 , and an antibody minimum recognition unit.5. The binding protein according to claim 1 , wherein the binding protein further comprises an antibody constant region sequence.6. An isolated nucleic acid encoding the binding protein according to .7. A vector comprising the nucleic ...
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