MEDICAL COMPOSITION FOR TREATMENT OR PROPHYLAXIS OF GLAUCOMA

The present invention is to provide a medical composition for the treatment or prophylaxis of glaucoma which comprises a pyridylaminoacetic acid compound represented by the formula (1): 2. The medical composition according to claim 1 , wherein Rand Reach independently represent a hydrogen atom or a C-Calkyl group.3. The medical composition according to or claim 1 , wherein Y represents a bicyclic heteroaromatic ring group or a group -Q-Qeach of which may be substituted by a group(s) selected from the group consisting of a halogen atom claim 1 , C-Calkyl group claim 1 , halogeno C-Calkyl group claim 1 , C-Calkoxyl group claim 1 , halogeno C-Calkoxyl group and C-Calkylthio group claim 1 ,{'sup': 1', '2, 'sub': 1', '4', '1', '4', '1', '4', '1', '4, 'wherein Qrepresents an arylene group or a 5- to 6-membered heteroarylene group, Qrepresents an aromatic group or a 5- to 6-membered ring heterocyclic group each of which may be substituted by a group(s) selected from the group consisting of a halogen atom, hydroxyl group, C-Calkyl group, halogen C-Calkyl group, C-Calkoxyl group and halogeno C-Calkoxyl group.'}4. The medical composition according to any one of to claim 1 , wherein Z represents an aromatic group or a completely unsaturated 5- to 6-membered heteroaromatic ring group each of which may be substituted by a group(s) selected from the group consisting of a halogen atom claim 1 , C-Calkyl group claim 1 , halogeno C-Calkyl group claim 1 , C-Calkoxyl group and halogeno C-Calkoxyl group.5. The medical composition according to any one of to claim 1 , wherein Y represents a benzofuryl group claim 1 , benzothienyl group claim 1 , benzoxazolyl group or benzothiazolyl group claim 1 , or group -Q-Qeach of which may be substituted by a group(s) selected from the group consisting of a halogen atom claim 1 , C-Calkyl group claim 1 , halogeno C-Calkyl group claim 1 , C-Calkoxyl group claim 1 , halogeno C-Calkoxyl group and C-Calkylthio group claim 1 ,{'sup': 1', '2, 'sub': 1', ' ...

SUBSTITUTED CARBONYL COMPOUND

This is to provide a novel substituted carbonyl compound represented by the following formula (I) having an excellent bronchodilatory action based on potent EP2 agonistic action and useful for treatment of respiratory diseases. 2. The substituted carbonyl compound or a pharmaceutically acceptable salt thereof according to claim 1 , wherein Rand Reach independently represent a hydrogen atom or a C-Calkyl group.3. The substituted carbonyl compound or a pharmaceutically acceptable salt thereof according to claim 1 , wherein Rrepresents a group —OR(wherein Rrepresents a C-Calkyl group; a C-Caralkyl group; a halogeno-C-Calkyl group; a C-Ccycloalkyl group which may be substituted by a group(s) selected from the group consisting of a halogeno group claim 1 , a C-Calkyl group claim 1 , a halogeno-C-Calkyl group and a C-Calkylene group; or a C-Calkyl group which is substituted by a group(s) selected from the group consisting of a C-Ccycloalkyl group claim 1 , a C-Calkanoyloxy group claim 1 , an N claim 1 ,N-di(C-Calkyl)aminocarbonyl group claim 1 , a (C-Calkoxy)carbonyloxy group and a 5- to 6-membered heterocyclic group) claim 1 , a group —O(CHCHO)R(wherein Rrepresents a hydrogen atom or a benzyl group claim 1 , and m is an integer of 3 to 4) claim 1 , or claim 1 , a group —NRR(wherein Rand Rmay be the same or different from each other claim 1 , and each represents a hydrogen atom claim 1 , a C-Calkyl group claim 1 , a halogeno-C-Calkyl group or a C-Ccycloalkyl group claim 1 , or claim 1 , Rand Rmay form a 4- to 6-membered ring in combination which may be substituted by a group(s) selected from the group consisting of a halogeno group claim 1 , a C-Calkyl group claim 1 , a halogeno-C-Calkyl group and a C-Calkylene group).5. The substituted carbonyl compound or a pharmaceutically acceptable salt thereof according to claim 1 , wherein Z represents a phenyl group claim 1 , a thienyl group claim 1 , an imidazolyl group claim 1 , a thiazolyl group claim 1 , a pyridyl group or a ...

NOVEL INDAZOLE DERIVATIVE OR SALT THEREOF AND PRODUCTION INTERMEDIATE THEREOF, AND ANTIOXIDANT USING SAME, AND USE OF INDAZOLE DERIVATIVE OR SALT THEREOF

A compound represented by formula (1) or salt thereof and a production intermediate thereof are created. The compound exhibited an excellent antioxidation action in a microsome lipid peroxidation measuring system using a rat liver microsome. Therefore, the compound or salt thereof is useful as an antioxidant. The present invention also provides use of a compound represented by the formula (1) or a pharmaceutically acceptable salt thereof for production of an antioxidant. The present invention relates to a novel indazole derivative or salt thereof, and a production intermediate thereof. The present invention also relates to an antioxidant containing at least one of the indazole derivative or salt thereof as an active ingredient. Further, the present invention relates to use of such an indazole derivative or salt thereof, for production of an antioxidant.Recently, it has been revealed that generation of lipid peroxide in a biological body and radical reaction accompanying the same cause various adverse affects on the biological body through a membrane disorder, cell disorder or the like. In association with this, various attempts of application of an antioxidant or a lipid peroxide generation suppressing agent to a medicine have been made, and many studies for antioxidants are made.For example, as representative antioxidants, vitamin C, vitamin E, polyphenol and the like are used in foods and cosmetics. Also SOD (superoxide dismutase) or the like being an enzyme that brings active oxygen into an oxygen molecule and a hydrogen peroxide molecule is well known as an antioxidant. Further, edaravone is used as a therapeutic agent for preventing enlargement of an infarction site after cerebral infarction by its antioxidation action, and probucol or the like which is a therapeutic agent for hyperlipidemia is known to suppress oxidation of LDL (low density lipoprotein) and have an arteriosclerosis suppressing action. However, not many of these are practically satisfactory ...

Substituted Pyridine Compound

The present invention provides a substituted pyridine compound or a pharmacologically acceptable salt thereof which has excellent CETP inhibition activity and is useful as a medicament. The present invention provides a compound represented by a general formula (I), 3. The compound of or a pharmacologically acceptable salt thereof claim 2 , wherein Ris a hydrogen atom claim 2 , a C-Calkyl group claim 2 , a hydroxy(C-Calkyl) group claim 2 , a (C-Calkoxy)-(C-Calkyl) group claim 2 , a (C-Calkyl)amino-(C-Calkyl) group claim 2 , a [N-(C-Calkyl)-N-hydroxy(C-Calkyl)amino]-(C-Calkyl) group claim 2 , a [N-(C-Calkyl)-N-(C-Calkyl)sulfonylamino]-(C-Calkyl) group claim 2 , a carboxy(C-Calkyl) group claim 2 , a halogeno(C-Calkyl) group claim 2 , a C-Calkenyl group claim 2 , a C-Ccycloalkyl group claim 2 , a C-Ccycloalkenyl group claim 2 , a hydroxy group claim 2 , a C-Calkoxy group claim 2 , a hydroxy(C-Calkoxy) group claim 2 , a (C-Calkyl)sulfonyl-(C-Calkoxy) group claim 2 , a carboxy(C-Calkoxy) group claim 2 , a halogeno(C-Calkoxy) group claim 2 , a C-Calkylthio group claim 2 , a C-Calkylsulfonyl group claim 2 , a N-(C-Calkyl)-N-hydroxy(C-Calkyl)amino group claim 2 , a (C-Calkylamino)carbonyl group claim 2 , a di(C-Calkyl)aminocarbonyl group claim 2 , a cyano group or a halogeno group.4. The compound of or a pharmacologically acceptable salt thereof claim 2 , wherein Ris a hydrogen atom claim 2 , a C-Calkyl group claim 2 , a hydroxy(C-Calkyl) group claim 2 , a (C-Calkoxy)-(C-Calkyl) group claim 2 , a halogeno(C-Calkyl) group claim 2 , a C-Calkoxy group claim 2 , a hydroxy(C-Calkoxy) group or a (C-Calkyl)sulfonyl-(C-Calkoxy) group.5. The compound of or a pharmacologically acceptable salt thereof claim 2 , wherein Ris a C-Calkyl group claim 2 , a halogeno(C-Calkyl) group claim 2 , a C-Calkoxy group or a hydroxy(C-Calkoxy) group.6. The compound of or a pharmacologically acceptable salt thereof claim 2 , wherein Ris a C-Calkyl group.7. The compound of or a pharmacologically ...

Substituted PyridineCompound

The present invention provides a substituted pyridine compound or a pharmacologically acceptable salt thereof which has excellent CETP inhibition activity and is useful as a medicament. The present invention provides a compound represented by a general formula (I), 3. The compound of or a pharmacologically acceptable salt thereof claim 2 , wherein Ris a hydrogen atom claim 2 , a C-Calkyl group claim 2 , a hydroxy(C-Calkyl) group claim 2 , a (C-Calkoxy)-(C-Calkyl) group claim 2 , a (C-Calkyl)amino-(C-Calkyl) group claim 2 , a [N—(C-Calkyl)-N-hydroxy(C-Calkyl)amino]-(C-Calkyl) group claim 2 , a [N—(C-Calkyl)-N—(C-Calkyl)sulfonylamino]-(C-Calkyl) group claim 2 , a carboxy(C-Calkyl) group claim 2 , a halogeno(C-Calkyl) group claim 2 , a C-Calkenyl group claim 2 , a C-Ccycloalkyl group claim 2 , a C-Ccycloalkenyl group claim 2 , a hydroxy group claim 2 , a C-Calkoxy group claim 2 , a hydroxy(C-Calkoxy) group claim 2 , a (C-Calkyl)sulfonyl-(C-Calkoxy) group claim 2 , a carboxy(C-Calkoxy) group claim 2 , a halogeno(C-Calkoxy) group claim 2 , a C-Calkylthio group claim 2 , a C-Calkylsulfonyl group claim 2 , a N—(C-Calkyl)-N-hydroxy(C-Calkyl)amino group claim 2 , a (C-Calkylamino)carbonyl group claim 2 , a di(C-Calkyl)aminocarbonyl group claim 2 , a cyano group or a halogeno group.4. The compound of or a pharmacologically acceptable salt thereof claim 2 , wherein Ris a hydrogen atom claim 2 , a C-Calkyl group claim 2 , a hydroxy(C-Calkyl) group claim 2 , a (C-Calkoxy)-(C-Calkyl) group claim 2 , a halogeno(C-Calkyl) group claim 2 , a C-Calkoxy group claim 2 , a hydroxy(C-Calkoxy) group or a (C-Calkyl)sulfonyl-(C-Calkoxy) group.5. The compound of or a pharmacologically acceptable salt thereof claim 2 , wherein Ris a C-Calkyl group claim 2 , a halogeno(C-Calkyl) group claim 2 , a C-Calkoxy group or a hydroxy(C-Calkoxy) group.6. The compound of or a pharmacologically acceptable salt thereof claim 2 , wherein Ris a C-Calkyl group.7. The compound of or a pharmacologically ...

CELL CULTURING METHOD AND KIT

The present invention relates to a cell culturing method and kit. More specifically, it relates to a cell culturing method and kit using a support that is exposed to the air. It further relates to a method of culturing cells by allowing them to migrate onto a porous polyimide film. 1. A cell culturing method including:(1) a step of supporting cells on one or more sheet-like porous supports,(2) a step of applying a medium to the sheet-like porous support supporting the cells and wetting the sheet-like porous support with the medium, with the medium included in some or all of the pores of the sheet-like porous support,(3) a step of disposing the sheet-like porous support in a culturing vessel that houses medium, in such a manner that all or a portion of the surface of the sheet-like porous support wetted with the medium is exposed to a gas phase, and(4) a step of setting the culturing vessel in an incubator and culturing the cells,wherein the wetted state of the sheet-like porous support surface and interior is maintained throughout the culturing.2. A culturing method according to claim 1 , wherein in step (4) claim 1 , the medium is supplied into the culturing vessel in a continuous or intermittent manner claim 1 , and all or a portion of the sheet-like porous support wetted with the medium is exposed to a gas phase throughout the culturing.3. A method according to or claim 1 , wherein in step (4) claim 1 , the step of culturing the cells is a step of culturing while supplying oxygen by oxygen-supplying means.4. A method according to any one of to claim 1 , wherein in step (3) claim 1 , the sheet-like porous support is mounted on a rigid body.5. A method according to claim 4 , wherein the rigid body is a metal mesh.6. A method according to any one of to claim 4 , wherein in step (3) claim 4 , a porous sheet with a larger mean pore size than the sheet-like porous supports is mounted in such a manner as to cover all or a portion of the top surface of the one or more ...

METHOD FOR ISOLATING, REMOVING AND ANALYZING CELLS

The purpose of the present invention is to provide a method for isolating, removing and analyzing cells. Provided is a method for isolating, removing and analyzing cells by filtering a liquid specimen containing cells through a porous polyimide film, and subjecting the cells captured by the film which did not pass through the porous polyimide film, or the cells in the liquid specimen which did pass through the porous polyimide film, to an examination of one or more cell properties selected from a group consisting of cell number or type, internal or external cell structure, type or amount of cell surface antigen, type or amount of material secreted from cells, cell adhesion, and cell survival rate. 1. A method for isolating , removing and/or analyzing cells , including: filtering a liquid sample containing cells with a polyimide porous film , and examining one or more cell properties selected from the group consisting of the number or type of cells , external or internal structure of the cells , type or amount of cell surface antigens , type or amount of substances secreted from the cells , cell adhesion and cell survival rate for cells that were captured in the polyimide porous film without being filtered by the film as well as cells in the liquid sample that passed through the polyimide porous film.2. The method according to claim 1 , wherein the target cells are activated cells.3. The method according to claim 2 , wherein the activated cells are activated leukocytes.4. The method according to any one of to claim 2 , which includes a step for pretreating all or a portion of the surface of the polyimide porous film prior to the filtration step.5. The method according to claim 4 , wherein surface treatment of the polyimide porous film is carried out using one or more agents or treatment methods selected from the group consisting of an anticoagulant claim 4 , collagen claim 4 , poly-L-lysine claim 4 , UV light claim 4 , plasma irradiation and PDS bound to a ...

METHOD, DEVICE AND KIT FOR MASS CULTIVATION OF CELLS USING POLYIMIDE POROUS MEMBRANE

The present invention pertains to a method for the mass cultivation of cells, and a cell cultivation device and kit. The present invention further pertains to a continuous cell cultivation method and a continuous cell cultivation device in which a carrier is used. 1. A mass cell culturing method including:(1) applying cells to a porous polyimide film, and(2) applying the porous polyimide film to which the cells have been applied, to a cell culture medium and performing culturing.2. The method according to claim 1 , wherein two or more porous polyimide films layered either above and below or left and right are used in the cell culture medium.3. The method according to or claim 1 , wherein the porous polyimide films are:i) folded,ii) wound into a roll,iii) connected as sheets or fragments by a filamentous structure, oriv) bound into a rope,to be suspended or fixed in the cell culture medium in the cell culturing vessel.4. The method according to any one of to claim 1 , wherein in the culturing of step (2) claim 1 , all or some of the porous polyimide films are not in contact with the liquid phase of the cell culture medium.5. The method according to any one of to claim 1 , wherein in the culturing of step (2) claim 1 , the total volume of the cell culture medium in the cell culturing vessel is 10 claim 1 ,000 times or less of the total sum of the porous polyimide film volume including the cell survival zone.6. The method according to any one of to claim 1 , wherein in the culturing of step (2) claim 1 , the total volume of the cell culture medium in the cell culturing vessel is 100 times or less of the total sum of the porous polyimide film volume including the cell survival zone.7. The method according to any one of to claim 1 , wherein the culturing in step (2) is carried out in a system in which a cell culture medium is continuously or intermittently supplied to a cell culturing vessel from cell culture medium supply means installed outside of the cell culturing ...

METHOD OF PRODUCING SUBSTANCE

The present invention relates to a method of using cells to produce a substance, and involves applying the cells to a polyimide porous film, culturing the cells and producing the substance by means of the cells. 1. A method of producing a substance using cells , the method including applying the cells to a porous polyimide film and culturing the cells to produce the substance by the cells.2. The method according to claim 1 , wherein the substance is selected from the group consisting of proteins claim 1 , glycoproteins and viruses.3. The method according to or claim 1 , wherein the cells are cultured under stationary culture conditions.4. The method according to or claim 1 , wherein the cells are cultured under rotating culture or stirred conditions.5. The method according to or claim 1 , wherein the cells are cultured in a continuous manner.6. The method according to claim 5 , including:setting a cell culturing apparatus in an incubator and culturing the cells, whereinthe cell culturing apparatus includes:a culturing unit that houses one or more porous polyimide films to support cells, and that comprises a culture medium supply port and a culture medium discharge port, anda culture medium-supply unit comprising a culture medium housing vessel, a culture medium supply line, and a liquid conveyance pump that conveys the medium through the culture medium supply line, the first end of the culture medium supply line contacting the medium in the culture medium housing vessel, and the second end of the culture medium supply line being in communication with the culturing unit interior via the culture medium supply port of the culturing unit.7. The method according to claim 6 , wherein the cell culturing apparatus does not have an air supply port claim 6 , an air discharge port and an oxygen permeation film.8. The method according to any one of to claim 6 , wherein the porous polyimide film is a porous polyimide film comprising a polyimide obtained from a tetracarboxylic ...

Cell culturing method, cell culturing apparatus and kit

The present invention relates to a cell culturing method as well as a cell culturing apparatus and kit for use in said culturing method. This cell culturing method comprises applying cells on a porous polyimide film and culturing.

CELL CULTURING METHOD, CELL CULTURING APPARATUS AND KIT

The present invention relates to a cell culturing method as well as a cell culturing apparatus and kit for use in said culturing method. This cell culturing method comprises applying cells on a porous polyimide film and culturing. 1. A cell culturing method that includes applying cells to a porous polyimide film and culturing them.220.-. (canceled)21. A cell culturing apparatus for use in a cell culturing method according to claim 1 , including a porous polyimide film.22. A cell culturing apparatus according to claim 21 , wherein two or more porous polyimide films are layered either above and below or left and right.23. (canceled) The present application claims priority based on Japanese Patent Application No. 2013-155550 submitted on Jul. 26, 2013, the entire contents of which are incorporated herein by reference.The present invention relates to a cell culturing method, and to a cell culturing apparatus and kit.Cells generally exist as three-dimensional aggregates in the body, but in classical plate culturing, cells are cultured in a monolayer fashion with the cells attached to a vessel. Numerous reports have indicated significant differences in cell properties with different culturing environments. An alternative is suspension culturing in which cells are cultured in a liquid culturing medium, but some cells are suited for suspension culture while others are not.The NanoCulture® Plate (NCP) developed by SCIVAX Corporation is an adhesive-type three-dimensional culturing multiplate patterned with an extracellular matrix (microspheres or microhoneycomb) on the bottom surface by nanoimprint technology. A regular repeating structure is adopted, which is quadrilateral for microspheres and hexagonal for microhoneycombs. The cells utilize this micropattern as a scaffold to actively form spheroids (numerous cells aggregated into a three-dimensional structure).Also, there have been reports of using cell culture sheets with regular arrangements of numerous uniform superfine ...

CELL CULTURE DEVICE, AND CELL CULTURE METHOD USING SAME

The present invention provides a cell culture device characterized by comprising: a culture container; a rotating polymeric porous film housing part which is housed in the culture container, and has one or more culture medium inlet/outlet openings; and a polymeric porous film which is housed in the rotating polymeric porous film housing part, wherein the polymeric porous film is a modular polymeric porous film, and the rotating polymeric porous film housing part is rotated independently of the culture container. 1. A cell culture device comprising:a culture vessel;a rotary porous polymer film container that is contained in the culture vessel and has one or more medium flow inlets; anda porous polymer film contained in the rotary porous polymer film container;wherein the porous polymer film is a porous polymer film with a three-layer structure, comprising a surface layer A and a surface layer B having a plurality of pores, as well as a macrovoid layer sandwiched between the surface layer A and the surface layer B; an average pore diameter of the pores present in the surface layer A is smaller than an average pore diameter of the pores present in the surface layer B; the macrovoid layer comprises a partition wall bonded to surface layers A and B, and a plurality of macrovoids surrounded by the partition wall and the surface layers A and B; and the pores in the surface layers A and B communicate with the macrovoids;wherein the porous polymer film is a modularized porous polymer film; andwherein the rotary porous polymer film container is rotated independently of the culture vessel.2. The cell culture device according to claim 1 , further comprising rotational driving means for rotating the rotary porous polymer film container.3. The cell culture device according to claim 2 , wherein the rotary porous polymer film container comprises rotative power reception means.4. The cell culture device according to claim 3 , wherein the rotative power reception means is a magnet.5. ...

METHOD FOR INHIBITING DIFFERENTIATION OF NEURAL STEM CELL, METHOD FOR PREPARING NEURAL STEM CELL, AND METHOD FOR DIFFERENTIATING AND INDUCING NEURAL STEM CELL

The present invention relates to: a method for inhibiting the differentiation of a neural stem cell by culturing the neural stem cell on a polymer porous membrane; a method for preparing a neural stem cell; and a method for differentiating and inducing a neural stem cell. 1. A method for inhibiting differentiation of neural stem cells comprising the steps:(1) applying neural stem cells to a porous polymer film; and(2) culturing and proliferating the neural stem cells,wherein the porous polymer films are a three-layer structure porous polymer film having a surface layer A and a surface layer B, the surface layers having a plurality of pores, and a macrovoid layer sandwiched between the surface layers A and B, wherein an average pore diameter of the pores present in the surface layer A is smaller than an average pore diameter of the pores present in the surface layer B; wherein the macrovoid layer has a partition wall bonded to the surface layers A and B, and a plurality of macrovoids surrounded by such a partition wall and the surface layers A and B; and wherein the pores in the surface layers A and B communicate with the macrovoid.2. The method according to claim 1 , wherein the neural stem cells are wild-type cells.3. The method according to or claim 1 , wherein the step (2) is implemented for at least 70 days.4. The method according to any one of to claim 1 , wherein the step (2) is implemented until the neural stem cells have proliferated to at least 1.0×10per square centimeter of the porous polymer film.5. The method according to any one of to claim 1 , using two or more porous polymer films layered either above and below or left and right in the cell culture medium.6. The method according to any one of to claim 1 , wherein the porous polymer film is:i) folded;ii) wound into a roll;iii) connected as sheets or fragments by a filamentous structure, oriv) bound into a rope,and suspended or anchored in a cell culture medium in a cell culturing vessel.7. The method ...

METHOD FOR PRODUCING HETEROAROMATIC SULFONAMIDE COMPOUND

The present invention provides: a method for producing a mixed sulfonic acid anhydride of the general formula (1) by reacting a sulfonic acid compound of the general formula (2) with a perfluoroalkyl sulfonic acid anhydride or a perfluoroalkyl sulfonic acid halide; and a method for producing a sulfonamide compound of the general formula (4) obtained by reacting an amine compound of the general formula (3) with a mixed sulfonic acid anhydride of the general formula (1). 2. The method for producing a mixed sulfonic acid anhydride according to claim 1 , wherein the sulfonylating agent is a perfluoroalkyl sulfonic acid anhydride having 1 to 4 carbon atoms or a perfluoroalkyl sulfonic acid halide having 1 to 4 carbon atoms.3. The method for producing a mixed sulfonic acid anhydride according to claim 1 , wherein the sulfonylating agent is trifluoromethanesulfonic acid anhydride claim 1 , pentafluoroethanesulfonic acid anhydride claim 1 , heptafluoropropanesulfonic acid anhydride claim 1 , nonafluorobutanesulfonic acid anhydride claim 1 , trifluoromethanesulfonic acid chloride claim 1 , pentafluoroethanesulfonic acid chloride claim 1 , heptafluoropropanesulfonic acid chloride or nonafluorobutanesulfonic acid chloride.4. The method for producing a mixed sulfonic acid anhydride according to claim 1 , wherein Ris a pyridyl group which may have a substituent.7. The method for producing a sulfonamide compound according to claim 5 , wherein Ris a pyridyl group which may have a substituent.8. The method for producing a sulfonamide compound according to claim 5 , wherein Ris an aralkyl group which may have a substituent.10. The method for producing a sulfonamide compound according to claim 5 , characterized in that the method is conducted at the reaction temperature of from 10 to 80° C.11. The method for producing a sulfonamide compound according to claim 5 , wherein a nitrile-based organic solvent claim 5 , a halogen-based organic solvent claim 5 , an aromatic amine-based ...

Substituted Pyridine Compound

The present invention provides a substituted pyridine compound or a pharmacologically acceptable salt thereof which has excellent CETP inhibition activity and is useful as a medicament. The present invention provides a compound represented by a general formula (I), 3. The compound of or a pharmacologically acceptable salt thereof claim 2 , wherein Ris a hydrogen atom claim 2 , a C-Calkyl group claim 2 , a hydroxy(C-Calkyl) group claim 2 , a (C-Calkoxy)-(C-Calkyl) group claim 2 , a (C-Calkyl)amino-(C-Calkyl) group claim 2 , a [N—(C-Calkyl)-N-hydroxy(C-Calkyl)amino]-(C-Calkyl) group claim 2 , a [N—(C-Calkyl)-N—(C-Calkyl)sulfonylamino]-(C-Calkyl) group claim 2 , a carboxy(C-Calkyl) group claim 2 , a halogeno(C-Calkyl) group claim 2 , a C-Calkenyl group claim 2 , a C-Ccycloalkyl group claim 2 , a C-Ccycloalkenyl group claim 2 , a hydroxy group claim 2 , a C-Calkoxy group claim 2 , a hydroxy(C-Calkoxy) group claim 2 , a (C-Calkyl)sulfonyl-(C-Calkoxy) group claim 2 , a carboxy(C-Calkoxy) group claim 2 , a halogeno(C-Calkoxy) group claim 2 , a C-Calkylthio group claim 2 , a C-Calkylsulfonyl group claim 2 , a N—(C-Calkyl)-N-hydroxy(C-Calkyl)amino group claim 2 , a (C-Calkylamino)carbonyl group claim 2 , a di(C-Calkyl)aminocarbonyl group claim 2 , a cyano group or a halogeno group.4. The compound of or a pharmacologically acceptable salt thereof claim 2 , wherein Ris a hydrogen atom claim 2 , a C-Calkyl group claim 2 , a hydroxy(C-Calkyl) group claim 2 , a (C-Calkoxy)-(C-Calkyl) group claim 2 , a halogeno(C-Calkyl) group claim 2 , a C-Calkoxy group claim 2 , a hydroxy(C-Calkoxy) group or a (C-Calkyl)sulfonyl-(C-Calkoxy) group.5. The compound of or a pharmacologically acceptable salt thereof claim 2 , wherein Ris a C-Calkyl group claim 2 , a halogeno(C-Calkyl) group claim 2 , a C-Calkoxy group or a hydroxy(C-Calkoxy) group.6. The compound of or a pharmacologically acceptable salt thereof claim 2 , wherein Ris a C-Calkyl group.7. The compound of or a pharmacologically ...

METHOD FOR PRODUCING EXOSOME

The present invention provides a method for producing an exosome, comprising a step of applying a cell to a cell culture module to culture the cell and a step of producing an exosome by the cell, wherein the cell culture module is provided with a polymer porous film and a casing having at least two culture medium in-flow/out-flow ports and having the polymer porous film placed therein. 1. A method for producing an exosome using a cell , the method comprising the steps of:applying the cell to a cell culture module to culture the cell; andallowing the cell to produce the exosome, a porous polymer membrane; and', 'a casing having two or more medium flow inlets/outlets and containing the porous polymer membrane., 'wherein the cell culture module comprises2. The method according to claim 1 ,wherein the porous polymer membrane is a three-layer structure porous polymer membrane having a surface layer A and a surface layer B, the surface layers having a plurality of pores, and a macrovoid layer sandwiched between the surface layers A and B,wherein an average pore diameter of the pores present in the surface layer A is smaller than an average pore diameter of the pores present in the surface layer B,wherein the macrovoid layer has a partition wall bonded to the surface layers A and B, and a plurality of macrovoids surrounded by the partition wall and the surface layers A and B,wherein the pores in the surface layers A and B communicate with the macrovoid, andwherein the porous polymer membrane is contained within the casing with:(i) the two or more independent porous polymer membranes being aggregated;(ii) the porous polymer membranes being folded up;(iii) the porous polymer membranes being wound into a roll-like shape; and/or(iv) the porous polymer membranes being tied together into a rope-like shape.3. The method according to or claim 1 , wherein the diameter of the medium flow inlet/outlet is larger than the diameter of the cell claim 1 , and smaller than the diameter at ...

CELL CULTURING METHOD USING SMALL-PIECE POROUS MEMBRANE

The purpose of the present invention is to provide a cell culturing method using a small-piece polymer porous membrane. The present invention provides a cell culturing method which does not require stirring and comprises applying cells to a small-piece polymer porous membrane having an area of a surface layer A or B of at most 4 mm2, and culturing the cells. The small-piece polymer porous membrane has a characteristic three-dimensional structure, and a medium can be more easily supplied and recovered by utilizing the property that small-piece polymer porous membranes are dispersed in a cell culture solution and/or small-piece polymer porous membranes are accumulated in multiple layers, and thus the small-piece polymer porous membranes are dispersed and/or laminated on a bottom part of a cell culture vessel. Consequently, long-term culture and mass culture are achieved, and thus the stable long-term production of exosomes is possible. 1. A cell culturing method which comprises applying cells to small-piece polymer porous membranes and culturing them , and which does not require stirring , wherein:the small-piece polymer porous membranes are small-piece polymer porous membranes with a three-layer structure comprising a surface layer A and a surface layer B having numerous pores, and a macro-void layer sandwiched between the surface layer A and surface layer B, the mean pore size of the pores in the surface layer A being smaller than the mean pore size of the pores in the surface layer B, the macro-void layer having partitions bonded to the surface layers A and B and numerous macro-voids surrounded by the partitions and the surface layers A and B, and the pores of the surface layers A and B communicating with the macro-voids,{'sup': '2', 'the area of the surface layer A or surface layer B is 4 mmor smaller, and'}the small-piece polymer porous membranes are dispersed and/or the small-piece polymer porous membranes are aggregated together in layers in the culture ...

Cell culture method using bone marrow-like structure, and polyimide porous membrane for treating bone damage site

The present invention relates to a method for culturing bone marrow cells, in which bone marrow cells are applied to a porous polyimide film and cultured. Moreover, the present invention relates to a porous polyimide film for healing a bone injury site.

CELL CULTURING METHOD AND KIT

The present invention relates to a cell culturing method and kit. More specifically, it relates to a cell culturing method and kit using a support that is exposed to the air. It further relates to a method of culturing cells by allowing them to migrate onto a porous polyimide film. 1. A method of culturing cells , the method including:contacting a first porous polyimide film with a cell-containing sample, to allow the cells to migrate from the cell-containing sample onto the first porous polyimide film; andculturing the cells that have been allowed to migrate onto the first porous polyimide film, wherein the first porous polyimide film has a multilayer structure having at least two surface layers (an A-surface and a B-surface), and a macro-void layer sandwiched between the two surface layers, a mean pore size of the holes in the A-surface is smaller than a mean pore size of the holes in the B-surface, and the macro-void layer has a partition bonded to the surface layers (the A-surface and the B-surface), and a plurality of macro-voids surrounded by the partition and the surface layers (the A-surface and the B-surface).2. The method according to claim 1 , wherein the cell-containing sample is a cell culture substrate in which cells are being cultured claim 1 , and wherein the cell culture substrate is selected from the group consisting of plates claim 1 , dishes claim 1 , culture plates claim 1 , culture flasks claim 1 , microwell plates and glass bottom dishes claim 1 , and the first porous polyimide film is contacted with the top surface of the cell culture substrate.3. The method according to claim 1 , wherein the cell-containing sample is a cell culture substrate in which cells are being cultured claim 1 , and wherein the cell culture substrate is selected from the group consisting of microcarriers claim 1 , silica porous bodies claim 1 , cellulose sponges claim 1 , nonwoven fabrics and hollow fibers claim 1 , and one or more first porous polyimide films are ...

CELL CULTURING METHOD, CELL CULTURING APPARATUS AND KIT

The present invention relates to a cell culturing method as well as a cell culturing apparatus and kit for use in said culturing method. This cell culturing method comprises applying cells on a porous polyimide film and culturing. One embodiment of the method according to the present invention comprises a process for sowing cells on the surface of the porous polyimide film. An embodiment comprises a process for placing a cell suspension on the dried surface of the porous polyimide film and leaving the porous polyimide film undisturbed or moving said porous polyimide film to promote liquid efflux or stimulating a portion of the surface to cause the cell suspension to be entangled into the film and then retaining the cells in the cell suspension inside the film while allowing the moisture to flow out. Another embodiment comprises a process for moistening one or both surfaces of the porous polyimide film with a cell culture solution or sterilized liquid, loading a cell suspension on the moistened porous polyimide film, then retaining the cells in the cell suspension inside the film, and allowing the moisture to flow out. 1. A cell culturing method that includes applying cells to a porous polyimide film and culturing them.2. The method according to claim 1 , including a step of seeding cells on the surface of a porous polyimide film.3. The method according to claim 1 , including a step of:placing a cell suspension on the dried surface of the porous polyimide film,allowing the porous polyimide film to stand, or moving the porous polyimide film to promote efflux of the liquid, or stimulating part of the surface to cause absorption of the cell suspension into the film, andretaining the cells in the cell suspension inside the film and allowing the water to flow out.4. The method according to claim 1 , including a step of:wetting one or both sides of the porous polyimide film with a cell culture medium or a sterilized liquid,loading a cell suspension into the wetted porous ...

METHOD, DEVICE AND KIT FOR MASS CULTIVATION OF CELLS USING POLYIMIDE POROUS MEMBRANE

The present invention pertains to a method for the mass cultivation of cells, and a cell cultivation device and kit. The present invention further pertains to a continuous cell cultivation method and a continuous cell cultivation device in which a carrier is used. 2. The cell culturing apparatus according to claim 1 , wherein the culturing unit further comprises an air supply port and an air discharge port claim 1 , or an oxygen exchange membrane.3. The cell culturing apparatus according to claim 2 , wherein the air supply port and the air discharge port are claim 2 , respectively claim 2 , a 5% COgas-containing air supply port and a 5% COgas-containing air discharge port.4. The cell culturing apparatus according to claim 1 , wherein the culturing unit further houses means for agitating the porous polyimide film.5. The cell culturing apparatus according to claim 1 , wherein the culturing unit further comprises a culture medium discharge line claim 1 , the first end of the culture medium discharge line being connected to the culture medium housing vessel claim 1 , the second end of the culture medium discharge line being in communication with the culturing unit interior via the culture medium discharge port of the culturing unit claim 1 , and the medium being able to circulate through the culture medium-supply unit and the culturing unit.6. The cell culturing apparatus according to claim 1 , wherein the culturing unit further comprises a culture medium discharge line claim 1 , the first end of the culture medium discharge line being connected to a culture medium collecting unit and the second end of the culture medium discharge line being in communication with the culturing unit interior via the culture medium discharge port of the culturing unit claim 1 , and the discharged medium can be collected in the culture medium collecting unit.7. The cell culturing apparatus according to claim 1 , further including means for shaking the culturing unit.8. The cell culturing ...

LONG-TERM CELL-CULTIVATION USING POLYIMIDE POROUS MEMBRANE AND CELL-CRYOPRESERVATION METHOD USING POLYIMIDE POROUS MEMBRANE

The invention relates to a long term cell culturing method and a cell culturing apparatus and kit that employ a porous polyimide film. The invention further relates to a cell cryopreservation method and kit employing the porous polyimide film. 2. The method according to claim 1 , wherein in step (1) claim 1 , the cells are seeded on the porous polyimide film to support the cells on the porous polyimide film.3. The method according to claim 1 , wherein in step (1) claim 1 , the cells are seeded and cultured on the porous polyimide film to support the cells on the porous polyimide film.4. The method according to claim 1 , which further includes claim 1 , following step (3) claim 1 ,(4) a step of placing the cell-supporting porous polyimide film under conditions in which the cells thaw, to thaw the cells supported on the porous polyimide film.5. The method according to claim 4 , which further includes claim 4 , following step (4) claim 4 ,(5) a step of applying the cell-thawed porous polyimide film to a cell culture medium and culturing the cells.6. The method according to claim 5 , wherein in step (5) claim 5 , the cells are cultured until the cultured cells proliferate even outside of the porous polyimide film.7. The method according to claim 5 , wherein in step (5) claim 5 , the one or more separate non-cell-supporting porous polyimide films are applied onto the cell culture medium together with the cell-thawed porous polyimide film claim 5 , and culturing is carried out to support the cells on the one or more separate porous polyimide films.8. The method according to claim 5 , which further includes claim 5 , following step (5) claim 5 ,(6) a step of placing all or a portion of the cell-supporting porous polyimide film under conditions in which the cells freeze, to freeze the cells supported on the porous polyimide film, and(7) a step of storing the cell-supporting porous polyimide film under conditions in which the frozen state is maintained.9. The method ...

CELL CULTURE METHOD USING BONE MARROW-LIKE STRUCTURE, AND POROUS POLYIMIDE FILM FOR HEALING BONE INJURY SITE

The present invention relates to a method for culturing bone marrow cells, in which bone marrow cells are applied to a porous polyimide film and cultured. Moreover, the present invention relates to a porous polyimide film for healing a bone injury site. 1. A method of culturing bone marrow cells that includes applying bone marrow cells to a porous polyimide film and culturing them ,wherein the porous polyimide film has a three-layer structure consisting of an A-surface layer having a plurality of pores, a B-surface layer having a plurality of pores, and a macro-void layer sandwiched between the two surface layers,a mean pore size in the A-surface layer is smaller than a mean pore size in the B-surface layer, andthe macro-void layer has a partition bonded to the A-surface layer and the B-surface layer, and a plurality of macro-voids surrounded by the partition, the A-surface layer, and the B-surface layer.2. The method according to claim 1 , wherein the bone marrow cells are marrow stromal cells or bone marrow-derived blood cells.3. The method according to claim 1 , wherein the bone marrow cells are cells harvested from mammalian bone marrow or primary cultured cells from cells harvested from mammalian bone marrow.4. The method according to claim 1 , further including differentiation of bone marrow cells to hematocytes by culturing.5. The method according to claim 1 , further including applying bone marrow cells to a porous polyimide film and then adding a differentiation-inducing accelerating substance and culturing claim 1 , to accelerate differentiation from the bone marrow cells to hematocytes in a manner specific to the spatial structure of the porous polyimide film.6. The method according to claim 4 , further including recovering the hematocytes.7. The method according to claim 4 , wherein the hematocytes are erythroid progenitor cells or erythrocytes.9. The method according to claim 8 , wherein the first cell group is selected from the group consisting of ...

Cell cultivation device and cell cultivation method using same

The present invention provides a cell cultivation device comprising: a polymer porous film; a cell cultivation part that accommodates the polymer porous film; a culture medium supply means that is positioned at an upper section of the cell cultivation part; and a culture medium recovery means that is positioned at a lower section of the cell cultivation part, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, the holes in the surface layers A and B are in communication with the macrovoids, and the cell cultivation part comprises a side part and a bottom part that has one or more culture medium discharge ports.

CELL PREPARATION METHOD, CELL CULTIVATION DEVICE, AND KIT

The present invention relates to a cell preparation method that includes a step in which cells are applied to a polyimide porous film and cultivated, wherein the polyimide porous film is a polyimide porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, and the polyimide porous film is produced by a method including the following steps: (1) a step in which a poly(amic acid) solution comprising poly(amic acid) and an organic polar solvent is flow cast in a film shape and the result is immersed in or brought into contact with a coagulation medium to create a porous film of poly(amic acid); and (2) a step in which the porous film of poly(amic acid) obtained in step (1) is heat-treated and imidized. 1. A method for preparing cells , the method comprising the step of: applying cells to a porous polyimide film and culturing the cells;wherein the porous polyimide film is a three-layer structure porous polyimide film having a surface layer A and a surface layer B, the surface layers having a plurality of pores, and a macrovoid layer sandwiched between the surface layers A and B;wherein an average pore diameter of the pores present in the surface layer A is smaller than an average pore diameter of the pores present in the surface layer B;wherein the macrovoid layer has a partition wall bonded to the surface layers A and B, and a plurality of macrovoids surrounded by the partition wall and the surface layers A and B;wherein the pores in the surface layers A and B communicate with the macrovoids; andwherein the polyimide porous is produced by a method comprising the steps of:(1) casting a poly(amic acid) solution consisting of a poly(amic acid) and an organic polar solvent into a film-like shape, and dipping in or bringing it into contact with a coagulating solvent to prepare a porous film of poly(amic acid); and(2) ...

CELL CULTURE SUPERNATANT FLUID DERIVED FROM LUNG TISSUE

The purpose of the present invention is to provide a highly safe and highly effective pharmaceutical composition and the like for the prevention and treatment of respiratory diseases. The present invention provides a pharmaceutical composition with which it is possible to effectively repair damaged lung tissue by including a cell culture supernatant fluid derived from lung tissue. The present invention also provides a method and a kit for using said culture supernatant fluid to induce differentiation into lung surfactant protein positive cells. 1. A culture supernatant fluid of cells derived from lung tissue.2. The culture supernatant fluid according to claim 1 , wherein the cells derived from lung tissue are spherical cells expressing one or more proteins claim 1 , or mRNA encoding the same claim 1 , selected from the group consisting of pulmonary surfactant protein or a precursor thereof claim 1 , CD44 and CD45.3. The culture supernatant fluid according to claim 2 , wherein the pulmonary surfactant protein is pulmonary surfactant protein C (SP-C).4. The pharmaceutical composition according to claim 2 , wherein the expression level of one or more of the proteins claim 2 , or mRNA encoding the same claim 2 , selected from the group consisting of CD34 and CD90 is 5% or less of the expression level of all proteins or all mRNA.5. A method for preparing a culture supernatant fluid of cells derived from lung tissue claim 2 , comprising:(1) a step for forming a cell suspension by suspending cells isolated from lung tissue in a medium,(2) a step for culturing the cell suspension followed by removing non-adherent cells,(3) a step for further culturing the remaining cells in a medium to form spherical cells, and(4) a step for recovering the cell culture supernatant fluid.6. A pharmaceutical composition for the treatment and/or prevention of respiratory diseases claim 1 , containing the culture supernatant fluid according to .7. The pharmaceutical composition according to ...

CELL CULTIVATION METHOD, SUSPENDED CELL ELIMINATION METHOD, AND METHOD TO KILL SUSPENDED CELLS

The present invention provides a cell cultivation method that includes: (1) a step in which a first culture medium including suspended cells is applied to a cell cultivation module; (2) a step in which a temperature at which cell cultivation is possible is maintained, and the cells are made to adsorb to the cell cultivation module; and (3) a step in which the cell cultivation module to which the cells are adsorbed is cultivated within a culture vessel with a second culture medium, wherein a polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, and the holes in the surface layers A and B are in communication with the macrovoids. 1. A method for culturing a cell , the method comprising the steps of:(1) applying a cell culture module to a first medium containing a cell;(2) maintaining a temperature at which the cell can be cultured, and adsorbing the cell to the cell culture module, and(3) culturing the cell culture module having the cell adsorbed, in a second medium in a culture vessel; a porous polymer film; and', 'a casing having two or more medium flow inlets, the casing containing the porous polymer film,, 'wherein the cell culture module comprisingwherein the porous polymer film is a three-layer structure porous polymer film having a surface layer A and a surface layer B, the surface layers having a plurality of pores, and a macrovoid layer sandwiched between the surface layers A and B;wherein an average pore diameter of the ...

SIPHON TYPE CULTIVATION METHOD

The present invention provides a cell cultivation device comprising a polymer porous film, and a cell cultivation method using said cell cultivation device. Provided is a cell cultivation device that has a siphon structure or a shishi-odoshi structure, said cell cultivation device comprising: a polymer porous film; a cell cultivation part that accommodates the polymer porous film; a liquid reservoir part that has the cell cultivation part stored in an interior thereof; a culture medium supply means that is positioned at an upper section of the liquid reservoir part; a culture medium discharge means that is positioned at a lower section of the liquid reservoir part; and a culture medium recovery means that recovers the culture medium that is collected in the liquid reservoir part and is intermittently expelled. 1. A cell culture device characterized by comprising:a porous polymer film;a cell culture part that accommodates the porous polymer film;a liquid storage part that stores the cell culture part therein;a medium supply means disposed on an upper part of the liquid storage part;an inverted U-shaped tube communicating with a bottom part of the liquid storage part;a medium recovery means provided below the other end of the inverted U-shaped tube; anda medium discharge means disposed on the medium recovery means;wherein the porous polymer films are a three-layer structure porous polymer film having a surface layer A and a surface layer B, the surface layers having a plurality of pores, and a macrovoid layer sandwiched between the surface layers A and B;wherein an average pore diameter of the pores present in the surface layer A is smaller than an average pore diameter of the pores present in the surface layer B;wherein the macrovoid layer has a partition wall bonded to the surface layers A and B, and a plurality of macrovoids surrounded by such a partition wall and the surface layers A and B; andwherein when the liquid surface of the medium supplied to the liquid ...

IMPLANT AND KIT FOR TREATMENT OF BONE LESION SITE, AS WELL AS METHOD FOR TREATING BONE LESION SITE

The present invention pertains to an implant for treatment of a bone lesion site, the implant including a polymer porous film and a layer containing a biocompatible material, wherein the polymer porous film is a polymer porous membrane of a three-layer structure having a porous surface layer A, a porous surface layer B, and a macrovoid layer interposed between the surface layer A and the surface layer B; the average pore diameter of the pores in surface layer A is smaller than the average pore diameter of the pores in surface layer B; the macrovoid layer has a partition joined to the surface layers A and B and a plurality of macrovoids enclosed by the partition and the surface layers A and B; and the pores in the surface layers A and B communicate with the macrovoids. 2. The implant according to claim 1 , wherein the layer containing the biocompatible material is arranged on the surface layer A of the porous polymer film.3. The implant according to or claim 1 , wherein the biocompatible material is a compound selected from the group consisting of collagen claim 1 , silicone claim 1 , fibronectin claim 1 , laminin claim 1 , polylysine claim 1 , polylactide claim 1 , polyglycolide claim 1 , polycaprolactone claim 1 , polyhydroxybutyrate claim 1 , polylactide-co-caprolactone claim 1 , polycarbonate claim 1 , biodegradable polyurethane claim 1 , polyetherester claim 1 , polyesteramide claim 1 , hydroxyapatite claim 1 , a complex of collagen and β-TCP claim 1 , and a combination thereof.4. The implant according to any one of to claim 1 , wherein the biocompatible material is collagen.5. The implant according to any one of to claim 1 , wherein an average pore diameter of the surface layer A is 0.01 to 50 μm.6. The implant according to any one of to claim 1 , wherein an average pore diameter of the surface layer B is 20 to 100 μm.7. The implant according to any one of to claim 1 , wherein a film thickness of the porous polymer film is 5 to 500 μm.8. The implant according ...

MULTIPLE FLOW PATH CELL CULTIVATION METHOD

Provided are a cell cultivation device comprising a polymer porous film, and a cell cultivation method using said cell cultivation device. The cell cultivation device comprises: two or more levels of parallel or antiparallel flat flow paths; a polymer porous film that is positioned in the flat flow paths; a culture medium supply means that is positioned at one end of the flat flow paths; a culture medium discharge means that is positioned at the other end of the flat flow paths; a head tank that is positioned at an upper section of the flat flow path of the uppermost level; a culture medium distribution means that distributes a culture broth from the head tank to the flat flow paths; and a culture medium recovery means that recovers the culture broth that is discharged from the culture medium discharge means. 1. A cell culture device characterized by comprising:two or more-step parallel planar flow paths;a porous polymer film disposed in the planar flow paths;a medium supplying means disposed at one end of each of the planar flow paths;a medium discharging means disposed at the other end of each of the planar flow paths;a head tank disposed at the top of the uppermost planar flow path;a medium distributing means for distributing the culture liquid from the head tank to each planar flow path; anda medium collecting means for collecting the culture liquid discharged from the medium discharging means;wherein the porous polymer films are a three-layer structure porous polymer film having a surface layer A and a surface layer B, the surface layers having a plurality of pores, and a macrovoid layer sandwiched between the surface layers A and B;wherein an average pore diameter of the pores present in the surface layer A is smaller than an average pore diameter of the pores present in the surface layer B;wherein the macrovoid layer has a partition wall bonded to the surface layers A and B, and a plurality of macrovoids surrounded by such a partition wall and the surface ...

Method to suppress stem cell differentiation, method to prepare stem cells, and method to induce differentiation of stem cells

The present invention relates to a method to suppress stem cell differentiation, said method including: (1) a step in which the stem cells are applied to a polymer porous film; and (2) a step in which the stem cells are cultivated and allowed to multiply, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, and the holes in the surface layers A and B are in communication with the macrovoids.

Method to suppress dedifferentiation of cells that readily dedifferentiate, method for preparing said cells, and method for producing substance

The present invention relates to a method to suppress the dedifferentiation of cells that readily dedifferentiate, said method including: (1) a step in which the cells are applied to a polymer porous film; and (2) a step in which the cells are cultivated and allowed to multiply, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, and the holes in the surface layers A and B are in communication with the macrovoids.

CELL CULTIVATION DEVICE AND CELL CULTIVATION METHOD USING SAME

The present invention provides a cell cultivation device that is characterized by comprising: a polymer porous film; a cell cultivation part that has the polymer porous film; a shaft that passes through the cell cultivation part; and a cultivation tank in which at least one portion of the cell cultivation part is immersed, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, the holes in the surface layers A and B are in communication with the macrovoids, the cell cultivation part rotates with the shaft as the center, and the cells are alternately cultivated in a gas phase and a liquid phase. 1. A cell culture apparatus , the apparatus characterized by comprising:a porous polymer film;a cell culture section having the porous polymer film;a shaft penetrating the cell culture section; anda medium tank immersing at least a part of the cell culture section;wherein the porous polymer films are a three-layer structure porous polymer film having a surface layer A and a surface layer B, the surface layers having a plurality of pores, and a macrovoid layer sandwiched between the surface layers A and B;wherein an average pore diameter of the pores present in the surface layer A is smaller than an average pore diameter of the pores present in the surface layer B;wherein the macrovoid layer has a partition wall bonded to the surface layers A and B, and a plurality of macrovoids surrounded by such a partition wall and the surface ...

Acid addition salt of substituted pyridine compound

The present invention provides a salt of a substituted pyridine compound which has excellent CETP inhibition activity and is useful as a medicament. The present invention provides a salt of a compound represented by general formula (I): wherein R 1 is optionally substituted alkoxy or the like.

CELL PREPARATION METHOD, CELL CULTIVATION DEVICE, AND KIT

The present invention relates to a cell preparation method that includes applying cells to a polyether sulfone porous film and cultivating the cells. 1. A method for preparing cells , the method comprising:applying cells to a porous polyethersulfone film and culturing them.2. The method according to claim 1 , wherein the porous polyethersulfone film is a three-layer structure porous polyethersulfone film having a surface layer A and a surface layer B claim 1 , the surface layers having a plurality of pores claim 1 , and a macrovoid layer sandwiched between the surface layers A and B;wherein an average pore diameter of the pores present in the surface layer A is smaller than an average pore diameter of the pores present in the surface layer B;wherein the macrovoid layer has a partition wall bonded to the surface layers A and B, and a plurality of macrovoids surrounded by the partition wall and the surface layers A and B; andwherein the pores in the surface layers A and B communicate with the macrovoids.3. The method according to or claim 1 , the method comprising the step of:seeding cells on the surface of the porous polyethersulfone film.4. The method according to or claim 1 , the method comprising the steps of:placing a cell suspension on the dried surface of the porous polyethersulfone film;allowing the porous polyethersulfone film to stand, or moving the porous polyethersulfone film to promote efflux of liquid, or stimulating a part of the surface to cause absorption of the cell suspension into the film; andretaining cells in the cell suspension inside the porous polyethersulfone film, and allowing water to flow out.5. The method according to or claim 1 , the method comprising the steps of:wetting one or both sides of the porous polyethersulfone film with a cell culture medium or a sterilized liquid;loading a cell suspension into the wetted porous polyethersulfone film; andretaining cells in the cell suspension inside the film, and allowing water to flow out.6. ...

CELL CULTIVATION MODULE

The present invention provides a cell cultivation module comprising a polymer porous film and a casing that has two or more culture medium inflow/outflow ports and accommodates the polymer porous film, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, the holes in the surface layers A and B are in communication with the macrovoids, and the polymer porous film is accommodated within the casing.

LONG-TERM CELL-CULTIVATION USING POLYIMIDE POROUS MEMBRANE AND CELL-CRYOPRESERVATION METHOD USING POLYIMIDE POROUS MEMBRANE

The invention relates to a long term cell culturing method and a cell culturing apparatus and kit that employ a porous polyimide film. The invention further relates to a cell cryopreservation method and kit employing the porous polyimide film. 1. A long term cell culturing method including:(1) applying cells to a porous polyimide film, and(2) applying the porous polyimide film to which the cells have been applied, to a cell culture medium and culturing the cells for 30 days or longer.2. The method according to claim 1 , wherein the cells are cultured for 60 days or longer in step (2).3. The method according to claim 1 , wherein the cells are cultured for 120 days or longer in step (2).4. The method according to any one of to claim 1 , using two or more porous polyimide films layered either above and below or left and right in the cell culture medium.6. The method according to any one of to claim 1 , wherein in the culturing of step (2) claim 1 , all or some of the porous polyimide films are not in contact with the liquid phase of the cell culture medium.7. The method according to any one of to claim 1 , wherein in the culturing of step (2) claim 1 , the total volume of the cell culture medium in the cell culturing vessel is 10 claim 1 ,000 times or less of the total sum of the porous polyimide film volume including the cell survival zone.8. The method according to any one of to claim 1 , wherein in the culturing of step (2) claim 1 , the total volume of the cell culture medium in the cell culturing vessel is 100 times or less of the total sum of the porous polyimide film volume including the cell survival zone.9. The method according to any one of to claim 1 , wherein the culturing in step (2) is carried out in a system in which a cell culture medium is continuously or intermittently supplied to a cell culturing vessel from cell culture medium supply means installed outside of the cell culturing vessel.10. The method according to claim 9 , wherein the cell culture ...

Medicinal composition for inhibiting increase in blood gastrin concentration

Cell cultivation device and cell cultivation method using same

Substituted carbonyl compound

Novel substituted carbonyl compounds represented by general formula (I) or salts thereof, which exhibit excellent bronchodilating effect that relies on potent EP2 agonist activity and which are useful in the treatment of respiratory diseases. In general formula (I), R1 is -OR5, -O(CH2CH2O)mR6 or -NR7R8 (wherein R5 is C7-22 alkyl or the like; R6 is H or benzyl; m is 1 to 4; and R7 and R8 are each H, C1-12 alkyl, or the like); R2 and R3 are each H or C1-6 alkyl; Y is a (substituted) bicyclic heteroaromatic ring, -Q1-Q2, or the like (wherein Q1 is arylene or the like; and Q2 is a (substituted) 5- or 6-memebred heterocycle, or the like); and Z is (substituted) aryl or a (substituted) 5- or 6-memeberd heteroaromatic ring.

Optically active pyrrolopyridazine compounds

An optically active pyrrolopyridazine derivative of the formula (I) or a pharmaceutically acceptable salt thereof: <CHEM> (wherein R<1> is alkyl; R<2> and R<3> are each independently alkyl; R<4> is optionally substituted aryl; and A is imino, oxygen or sulfur). ÄUtilityÜ The pyrrolopyridazine derivatives or pharmaceutically acceptable salts thereof of the present invention exhibit excellent gastric acid secretory inhibition activity and gastric mucous membrane protection activity, as well as excellent antibacterial activity against Helicobacter pylori. They are useful medicines and particularly useful for treating or preventing ulcerative diseases.

Process for Producing High-Purity Prasugrel and Acid Addition Salt Thereof

The present invention is directed to providing prasugrel hydrochloride or the like with a reduced content of OXTP. A method for producing prasugrel hydrochloride with a reduced content of OXTP, comprising dissolving free prasugrel containing OXTP in an inert solvent and adding hydrochloric acid optionally dropwise to the solution for reaction is also provided.

Pyrrolopyridazinone compound

The present invention discloses a pyrrolopyridazinone compound represented by the formula (1): wherein R 1 represents C 1 -C 2 alkyl group or halogeno C 1 -C 2 alkyl group, R 2 repersents C 3 -C 5 cycloalkyl group, (C 3 -C 5 cycloalkyl)C 1 -C 2 alkyl group or C 1 -C 3 alkyl group, R 3 represents hydrogen atom, or methylene group or cis-vinylene group for forming substituted oxygen-containing hetero ring in combination with group -O-R 2 , R 4 represents hydrogen atom, halogen atom, C 1 -C 8 alkyl group, C 2 -C 6 alkenyl group, C 2 -C 6 alkynyl group, hydroxy C 3 -C 6 alkenyl group, hydroxy C 3 -C 6 alkynyl group, C 1 -C 6 alkyl group substituted by substituent(s) selected from Substituent group (a), C 3 -C 6 cycloalkyl group which may be substituted by substituent(s) selected from Substituent group (b), "C 1 -C 3 alkyl group which is substituted by C 3 -C 6 cycloalkyl group which may be substituted by substituent(s) selected from Substituent group (b), and which may be substituted by a hydroxy group", an aromatic ring group or heteroaromatic ring group each of which may be substituted by a substituent(s) selected from Substituent group (c) or "C 1 -C 2 alkyl group which is substituted by aromatic ring group or heteroaromatic ring group each of which may be substituted by group(s) selected from Substituent group (c), and which may be substituted by a hydroxy group", Substituent group (a) represents a halogen atom, hydroxy group, cyano group, carboxy group, C 1 -C 5 alkoxy group, halogeno C 1 -C 4 alkoxy group, C 3 -C 6 cycloalkoxy group, (C 3 -C 6 cycloalkyl) C 1 -C 2 alkoxy group, C 1 -C 4 alkoxycarbonyl group, C 2 -C 4 alkanoyl group, C 2 -C 4 alkanoyloxy group or C 1 -C 4 alkyl-substituted amino group, Substituent group (b) represents a hydroxy group or a halogen atom, Substituent group (c) represents a halogen atom, a hydroxy group, a cyano group, a nitro group, a carboxy group, C 1 -C 5 alkoxy group, C 1 -C 4 alkoxycarbonyl group, C 2 -C 4 ...

Cell culture method using bone marrow-like structure, and porous polyimide film for healing bone injury site

The present invention relates to a method for culturing bone marrow cells, in which bone marrow cells are applied to a porous polyimide film and cultured. Moreover, the present invention relates to a porous polyimide film for healing a bone injury site.

Novel indazole derivative

An object of the present invention is to create a novel indazole derivative useful as a drug and to find a novel pharmacological action of the derivative. The compound of the present invention is represented by the formula [I] and has an excellent Rho kinase inhibiting action. In the formula, a ring X is a benzene ring or a pyridine ring; R 1 and R 2 are H or alkyl; R 3 and R 4 are halogen, H, OH, alkoxy, alkenyloxy, alkynyloxy, cycloalkyloxy, cycloalkenyloxy, aryloxy, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, aryl, carboxy, hydrocarbonyl, alkylcarbonyl, etc.; and R 5 is halogen atom, H, OH, alkoxy, aryloxy, alkyl or aryl. Each group can be substituted.

Siphon type cultivation method

cell culture kit and method

a presente invenção refere-se a um método e kit de cultura de célula. mais especificamente, refere-se a um método e kit de cultura de célula usando um suporte que é exposto ao ar. refere-se ainda a um método de cultura de células, permitindo-lhes migrar para uma película de poli-imida porosa.

Cell culturing method and kit

The present invention relates to a cell culturing method and kit. More specifically, it relates to a cell culturing method and kit using a support that is exposed to the air. It further relates to a method of culturing cells by allowing them to migrate onto a porous polyimide film.

Cell culture method using bone marrow-like structure, and porous polyimide film for healing bone injury site

The present invention relates to a method for culturing bone marrow cells, in which bone marrow cells are applied to a porous polyimide film and cultured. Moreover, the present invention relates to a porous polyimide film for healing a bone injury site.

Novel indazole derivative or salt thereof and production intermediate thereof, and antioxidant using same, and use of indazole derivative or salt thereof

Disclosed are: the compound represented by formula (1) or a salt thereof; and a manufacturing intermediate therefor. Said compound exhibits excellent antioxidant activity in a microsomal lipid peroxidation measurement system using rat-liver microsomes. Said compound, or a salt thereof, is useful as an antioxidant. Also disclosed is a use for the compound represented by formula (1), or a pharmacologically permitted salt thereof, for manufacturing an antioxidant.

Method of culturing cells using a porous polyimide film

The present invention relates to a method of using cells to produce a substance, and involves applying the cells to a polyimide porous film, culturing the cells and producing the substance by means of the cells.

Long-term cell-cultivation using polyimide porous membrane and cell-cryopreservation method using polyimide porous membrane

The invention relates to a long term cell culturing method and a cell culturing apparatus and kit that employ a porous polyimide film. The invention further relates to a cell cryopreservation method and kit employing the porous polyimide film.

Novel indazole derivative or salt thereof and production intermediate thereof, and antioxidant using same

Optically active pyrrolopyridazine compounds

Optically active pyrrolopyridazine compounds having the general formula (I) or pharmacologically acceptable salts thereof: (wherein R1 is alkyl; R2 and R3 are each independently alkyl; R4 is optionally substituted aryl; and A is imino, oxygen, or sulfur). The compounds and the salts exhibit an excellent inhibitory activity against the secretion of gastric hydrochloric acid, a protective activity for the gastric mucus, and an excellent antimicrobial activity against <i>Helicobacter pylori</i>, and are useful as drugs, particularly preventive or therapeutic agents for ulcerative diseases.

Benzazepinone compound

The present invention relates to a benzazepinone compound represented by the following formula (I): (see formula I) wherein R1 represents a C1-C6 alkyl group or halogeno C1-C6 alkyl group, R2 represents a carboxyl group which may be protected, and Y represents a group represented by the formula (II): (see formula II) wherein Z represents CH or a nitrogen atom, or a pharmaceutically acceptable salt thereof.

Acid addition salt of optically active dihydropyridine derivative

Acid addition salt of optically active dihydropyridine derivative

There is provided an excellent medicine for treating or preventing hypertension or the like. A specific acid addition salt of (R)-2-amino-1,4-dihydro-6-methyl-4-(3-nitrophenyl)-3,5-pyridinedicarboxylic acid 3-(1-diphenyl-methylazetidin-3-yl) ester 5-isopropyl ester is useful as a medicine for treating or preventing hypertension or the like.

New indazole derivatives that have spiro ring structure in side chain

Et formål med foreliggende oppfinnelse er å tilveiebringe et nytt indazolderivat som har en spiroringstruktur i en sidekjede, hvilket derivat er nyttig som et farmasøytisk preparat, samt å finne en ny farmakologisk aktivitet for derivatet. Foreliggende oppfinnelsesforbindelse er representert ved den generelle formelen (I) og har en utmerket Rho-kinaseinhiberende aktivitet. I formelen representerer ringen X en benzenring eller en pyridinring; R1 og R2 representerer et halogen, H, OH, alkoksy, cykoalkyloksy, aryloksy, alkyl, cykloalkyl eller lignende; R3 representerer halogen, H eller lignende; R4 og R5 representerer halogen, H eller lignende; R6 og R7 representerer H, alkyl eller lignende; og m, n, p og q representerer et heltall på 0 til 3. I denne sammenheng kan hver gruppe ha en substituent. (I)

Cell culture module

Provided is a means that applies uniform culture conditions to a plurality of porous polymer membranes and makes it possible to stably culture a large quantity of cells. A cell culture module that comprises: a top part; a bottom part; a side part that has a culture solution in/outflow port; a plurality of partition parts that partition a space that is formed by the top part, the bottom part, and the side part and have a culture solution in/outflow port; and porous polymer membranes that are respectively fixed in at least two interstitial spaces selected from the interstitial space between the top part and the adjacent partition part, the interstitial space between the bottom part and the adjacent partition part, and the plurality of interstitial spaces between adjacent partition parts. The porous polymer membranes have a three-layer structure that includes: a surface layer A and a surface layer B that have a plurality of pores; and a macrovoid layer that is sandwiched between surface layer A and surface layer B. The average pore diameter of the pores in surface layer A is smaller than the average pore diameter of the pores in surface layer B. The macrovoid layer has: a diaphragm that is bonded to surface layers A and B; and a plurality of macrovoids that are surrounded by the diaphragm and surface layers A and B. The pores in surface layers A and B communicate with the macrovoids.

Cell culture method using bone marrow-like structure, and porous polyimide film for healing bone injury site

The present invention relates to a method for culturing bone marrow cells, in which bone marrow cells are applied to a porous polyimide film and cultured. Moreover, the present invention relates to a porous polyimide film for healing a bone injury site.

Substituted pyridine compound

Disclosed is a substituted pyridine compound that has excellent CETP inhibiting activity and is useful as a pharmaceutical, or a pharmacologically acceptable salt thereof. Specifically disclosed is a compound, etc., that fulfills a general formula (I) (wherein, R1: H, substitutable alkyl, OH, optionally substituted alkoxy, alkylsulfonyl, optionally substituted amino, carboxy, optionally substituted carbonyl, CN, halogen, optionally substituted phenyl, optionally substituted aromatic heterocyclyl, optionally substituted saturated heterocyclyl, optionally substituted saturated heterocyclyloxy, optionally substituted saturated heterocyclylcarbonyl, etc.)

Pyrazole compounds

The present invention relates to pyrazole compounds represented by the formula (I): wherein R 1 represents phenyl which may be substituted, R 2 represents H, halogen, alkyl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl or substituted amino, Q represents CH or N, R 3 represents H, alkyl or amino, R 4 represents the formula (II) to (V): wherein R 7 represents H or alkyl, R 8 represents H, alkyl or substituted amino, R 9 represents H or alkyl, R 12 represents H, alkyl, halogeno alkyl or substituted amino, or pharmaceutically acceptable salts thereof, and a medical composition containing the same as effective ingredient.

Methods for treating a disease in which Rho kinase is involved

A method for treating a disease in which Rho kinase is involved. The method is carried out by administering to a patient in need thereof a pharmaceutically effective amount of a compound of the following formula or a pharmaceutically acceptable salt thereof: , wherein the ring X is a benzene ring or a pyridine ring; R 1 and R 2 are hydrogen or alkyl or together form a cycloalkene ring; R 3 is hydrogen, substituted alkyl, unsubstituted alkenyl, carboxyl or an ester or an amide thereof, amino or a cyano; R 4 is hydrogen, hydroxyl, substituted or unsubstituted alkoxy, unsubstituted alkenyloxy, unsubstituted cycloalkyloxy, substituted or unsubstituted alkyl, unsubstituted alkenyl, unsubstituted cycloalkyl, amino, substituted or unsubstituted alkylamino, nitro, cyano or a monocyclic heterocycle; and R 5 is a halogen atom or a hydrogen atom.

Pharmaceutical composition for treatment or prevention of ophthalmic diseases

A pharmaceutical composition for treating or preventing diseases associated with vascularization in eyes, which comprises a benzazepinone compound represented by structural formula (I) [wherein R represents a C-C alkyl group, or a halogeno-C-C alkyl group; R represents a carboxy group which may be protected; and Y represents a group represented by general formula (II) (wherein Z represents CH or a nitrogen atom)] or a pharmacologically acceptable salt thereof as an active ingredient.

Pyrrolopyridazine CONNECTIONS

Novel indazole derivative having spiro ring structure in side chain

A pharmaceutical composition for the treatment or prevention of glaucoma

Pyrrolopyridazine compound

Substituted pyridine compound

Un compuesto representado por la fórmula general (I) o una sal farmacéuticamente aceptable del mismo:**Fórmula** en la que R1 representa un átomo de hidrógeno, un grupo alquilo C1-C6, un grupo hidoxi (alquilo C1-C6), un grupo (alcoxi C1-C6)-(alquilo C1-C6), un grupo hidroxi(alcoxi C1-C6)-(alquilo C1-C6), un grupo (alquilo C1-C6)amino- (alquilo C1-C6), un grupo hidroxi(alquilo C1-C6)amino-(alquilo C1-C6), un grupo [N-(alquilo C1-C6)-N-hidroxi(alquilo C1-C6)amino]-(alquilo C1-C6), un grupo (alquilo C1-C6)sulfonilamino-(alquilo C1-C6), un grupo [N-(alquilo C1-C6)-N- (alquilo C1-C6)sulfonilamino]-(alquilo C1-C6), un grupo carboxi(alquilo C1-C6), un grupo halógeno(alquilo C1-C6), un grupo (cicloalquilo C3-C8)-(alquilo C1-C6), un grupo alquenilo C2-C6, un grupo alquinilo C2-C6, un grupo cicloalquilo C3-C8, un grupo cicloalquenilo C3-C8, un grupo hidroxi, un grupo alcoxi C1-C6, un grupo hidroxi(alcoxi C1-C6), un grupo (alcoxi C1-C6)-(alcoxi C1-C6), un grupo (alquilo C1-C6)sulfonil-(alcoxi C1-C6), un grupo carboxi(alcoxi C1-C6), un grupo halógeno(alcoxi C1-C6), un grupo alquiltio C1-C6, un grupo alquilsulfinilo C1-C6, un grupo alquilsulfonilo C1-C6, un grupo amino, un grupo alquilamino C1-C6, un grupo di(alquilo C1-C6)amino, un grupo hidroxi(alquilo C1-C6)amino, un grupo N-(alquilo C1-C6)-N-hidroxi(alquilo C1-C6)amino, un grupo formilamino, un grupo (alquilo C1-C6)carbonilamino, un grupo carboxi, un grupo (alcoxi C1-C6)carbonilo, un grupo carbamoílo, un grupo (alquilamino C1-C6)carbonilo, un grupo di(alquilo C1-C6)aminocarbonilo, un grupo ciano, un grupo halógeno, un grupo fenilo, un grupo fenilo sustituido, en el que el sustituyente o sustituyentes representan de 1 a 4 grupos seleccionados independientemente entre el grupo sustituyente α, un grupo heterociclilo aromático de 5 o 6 miembros, un grupo heterociclilo aromático de 5 o 6 miembros sustituidos, en el que el sustituyente o sustituyentes representan de 1 a 4 grupos seleccionados independientemente entre el grupo ...

Benzazepinone compound

The present invention relates to a benzazepinone compound represented by the following formula (I): wherein R 1 represents a C 1 -C 6 alkyl group or halogeno C 1 -C 6 alkyl group, R 2 represents a carboxyl group which may be protected, and Y represents a group represented by the formula (II): wherein Z represents CH or a nitrogen atom, or a pharmaceutically acceptable salt thereof.

Pyrrolopyridazine compound

A pyrrolopyridazine compound represented by the general formula (I) or a pharmacologically acceptable salt thereof. (In the formula, R1 is C2-6 alkenyl, C2-6 halogenoalkenyl, optionally substituted C3-7 cycloalkyl, or optionally substituted C1-6 alkyl substituted by C3-7 cycloalkyl; R2 is C1-6 alkyl; R3 is hydroxymethyl, C2-6 aliphatic acyloxymethyl, optionally substituted (C6-10 aryl)carbonyloxymethyl, (C1-6 alkoxy)carbonyloxymethyl, formyl, carboxy, (C1-6 alkoxy)carbonyl, or optionally substituted (C6-10 aryl)oxycarbonyl; R4 is optionally substituted C6-10 aryl; and A is imino, oxygen, or sulfur.) The pyrrolopyridazine compound is highly effective in inhibiting the secretion of gastric hydrochloric acid, protecting the gastri c mucosa, and the like. It is useful as a medicine, especially a preventive/remedy for ulcerative diseases.

Acid addition salt of optically active dihydropyridine derivative

An excellent medicine for the treatment or prevention of hypertension or the like is provided. A specific acid addition salt of (R)-2-amino-1,4-dihydro-6-methyl-4-(3-nitrophenyl)-3,5- pyridinedicarboxylic acid 3-(1-diphenylmethylazetidin-3- yl)ester 5-isopropyl ester is useful as a medicine for the treatment or prevention of hypertension or the like.

Acid Addition Salt of Dihydropyridine Derivative

There is provided an excellent medicine for treating or preventing hypertension or the like. A specific acid addition salt of 2-amino-1,4-dihydro-6-methyl-4-(3-nitrophenyl)-3,5-pyridinedicarboxylic acid 3-(1-diphenylmethylazetidin-3-yl)ester 5-isopropyl ester is useful as a medicine for treating or preventing hypertension or the like.

Substituted pyridine compound

Disclosed is a substituted pyridine compound that has excellent CETP inhibiting activity and is useful as a pharmaceutical, or a pharmacologically acceptable salt thereof. Specifically disclosed is a compound, etc., that fulfills a general formula (I) (wherein, R1: H, substitutable alkyl, OH, optionally substituted alkoxy, alkylsulfonyl, optionally substituted amino, carboxy, optionally substituted carbonyl, CN, halogen, optionally substituted phenyl, optionally substituted aromatic heterocyclyl, optionally substituted saturated heterocyclyl, optionally substituted saturated heterocyclyloxy, optionally substituted saturated heterocyclylcarbonyl, etc.)

Method for culturing cell, and method for removing and killing cell from cell suspension

The present invention provides a cell cultivation method that includes: (1) a step in which a first culture medium including suspended cells is applied to a cell cultivation module; (2) a step in which a temperature at which cell cultivation is possible is maintained, and the cells are made to adsorb to the cell cultivation module; and (3) a step in which the cell cultivation module to which the cells are adsorbed is cultivated within a culture vessel with a second culture medium, wherein a polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, and the holes in the surface layers A and B are in communication with the macrovoids.

Dibenzocycloheptene compound

A dibenzocycloheptene compound represented by the general formula (I): (I) (wherein R1 represents hydrogen, halogeno, etc.; R2 represents hydrogen, halogeno, etc.; A represents, e.g., a 5- or 6-membered heterocyclic aromatic group containing one to three heteroatoms selected from the group consisting of nitrogen, oxygen, and sulfur, the heterocyclic aromatic group optionally having halogeno, nitro, etc. as a substituent; B represents -CH=CH-, -CH2O-, etc.; Y represents C1-10 alkylene optionally having halogeno, etc. as a substituent; Z represents optionally protected carboxyl, etc.; m is an integ er of 1 to 4; n is an integer of 1 to 3; and ~~ indicates a single bond or doub le bond) or a pharmacologically acceptable salt of the compound; and a medicina l composition which contains the compound or salt as the active ingredient. Th e composition has not only strong leucotriene D4 antagonism but leucotriene C4 antagonism and leucotriene E4 antagonism. It is useful as an antasthmatic, antiallergic, or antiphlogistic.

Process for the preparation of highly pure prasugrel hydrochloride

Prasugrelhydroklorid redusert i OXTP-innhold. Det er tilveiebrakt en fremgangsmåte for fremstilling av prasugrelhydroklorid redusert i OXTP-innhold, som omfatter å oppløse OXTPholdig prasugrel i en fri form i et inert oppløsningsmiddel og dråpevis eller på annen måte tilsette saltsyre for å omsette dem. Prasugrel hydrochloride reduced in OXTP content. There is provided a process for preparing prasugrel hydrochloride reduced in OXTP content, which comprises dissolving OXTP-containing prasugrel in a free form in an inert solvent and dropwise or otherwise adding hydrochloric acid to react them.

Optically active pyrrolopyridazine compounds.

Method of producing substance

The present invention relates to a method of using cells to produce a substance, and involves applying the cells to a polyimide porous film, culturing the cells and producing the substance by means of the cells.

Pyrazole compounds

Process for producing high-purity prasugrel hydrochloride

Process for producing high-purity prasugrel and acid addition salt thereof

The present invention is directed to providing prasugrel hydrochloride or the like with a reduced content of OXTP. A method for producing prasugrel hydrochloride with a reduced content of OXTP, comprising dissolving free prasugrel containing OXTP in an inert solvent and adding hydrochloric acid optionally dropwise to the solution for reaction is also provided.

Cell cultivation module

The present invention provides a cell cultivation module comprising a polymer porous film and a casing that has two or more culture medium inflow/outflow ports and accommodates the polymer porous film, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, the holes in the surface layers A and B are in communication with the macrovoids, and the polymer porous film is accommodated within the casing.

Pyrrolopyridazinonverbindung als pde4 inhibitor

Method for producing exosome

Cell culture module

The present invention provides a cell cultivation module comprising a polymer porous film and a casing that has two or more culture medium inflow/outflow ports and accommodates 5 the polymer porous film, wherein the polymer porous film is a polymer porous film with a three layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that 10 are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, the holes in the surface layers A and B are in communication with the macrovoids, and the polymer porous film is accommodated within the casing.

5-alkoxy-2(3h)-oxazolonderivate und verfahren zu ihrer herstellung

ピロロピリダジン化合物酸付加塩

［構成］　７−（４−フルオロベンジルオキシ）−２,３−ジメチル−１−［（１Ｓ,２Ｓ）−２−メチルシクロプロピルメチル］ピロロ［２,３−ｄ］ピリダジンの塩酸塩、臭化水素酸塩、硫酸塩、硝酸塩、リン酸塩、ベンゼンスルホン酸塩、ｐ−トルエンスルホン酸塩、２,４−ジメチルベンゼンスルホン酸塩、２,４,６−トリメチルベンゼンスルホン酸塩、４−エチルベンゼンスルホン酸塩、２−ナフタレンスルホン酸塩、１／２フマル酸塩またはフタル酸塩。［効果］　本発明の７−（４−フルオロベンジルオキシ）−２,３−ジメチル−１−［（１Ｓ,２Ｓ）−２−メチルシクロプロピルメチル］ピロロ［２,３−ｄ］ピリダジンの酸付加塩は、強力な胃酸分泌抑制作用および胃粘膜保護作用ならびにヘリコバクター　ピロリー（Helicobacterpylori）に対する優れた抗菌作用を有し、医薬（特に、潰瘍性疾患の治療剤または予防剤）として有用である。

Cell culturing method using small-piece porous membrane

經取代之吡啶化合物的酸加成鹽

本發明之課題係提供一種具有優異CETP抑制活性而有用於作為醫藥之經取代之吡啶化合物之鹽。本發明提供一種以通式(I)表示之化合物之鹽：□[式中，R1：可經取代之烷氧基等]。

Method for producing exosome

The present invention provides a method for producing an exosome, comprising a step of applying a cell to a cell culture module to culture the cell and a step of producing an exosome by the cell, wherein the cell culture module is provided with a polymer porous film and a casing having at least two culture medium in-flow/out-flow ports and having the polymer porous film placed therein.

Composes de la 5-alcoxy-2-(3h)-oxazolone et leur procede de preparation

Acid addition salts of pyrrolopyridazine compound

Pyrrolopyridazine derivatives acid addition salts

Dibenzocycloheptene compound

5-alkoxy-2(3H)-oxazolone compounds and process for preparing the same

5-Alkoxy-2(3H)-oxazolone compounds represented by general formula (1); and a R 1 process for the preparation thereof, wherein R 1 is hydrogen, optionally substituted C 1 -C 10 alkyl, optionally substituted C 3 -C 10 cycloalkyl, optionally substituted C 2 -C 10 alkenyl or optionally substituted phenyl; R 2 is hydrogen, optionally substituted C 1 -C 10 alkyl, optionally substituted phenyl or unsubstituted C 2 -C 10 alkenyl; R 3 is optionally substituted C 1 -C 10 alkyl, optionally substituted C 3 -C 10 cycloalkyl, optionally substituted C 2 -C 10 alkenyl excluding 2-alkenyl, or optionally substituted phenyl. ##STR1##

Medicinal composition for treatment for or prevention of visceral pain