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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 3. Отображено 3.
16-09-2009 дата публикации

Preparation method of buffalo embryonic stem cell

Номер: CN0101531994A
Автор: SHI DESHUN, HUANG BEN, LI TONG, CUI KUIQING, DESHUN SHI, BEN HUANG, TONG LI, KUIQING CUI
Принадлежит:

The invention provides a preparation method of buffalo embryonic stem cells, comprising the following steps: obtaining buffalo blastulas and incubated blastulas by external fertilization, removing the zona pellucida and trophoblastic cells to obtain inner cell mass cells; using buffalo fetal fibroblasts processed by mitomycin as feeder layer cells; using DMEM added with fetal calf serum, non-essential amino acid, beta-mercaptoethanol, sodium pyruvic acid, recombinant mouse leukemia inhibitory factor, recombinant human basic fibrobast growth factor and recombinant mouse stem cell factor as culture solution to perform primary and sub-culture. The clone of the buffalo embryonic stem cells can be obtained using the preparation method. The preparation method proves that the buffalo embryonic stem cells have typical biological characteristic and differentiation potency of the embryonic stem cells.

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Номер записи: 1
05-05-2023 дата публикации

Method for extracting creamy globule RNA (Ribonucleic Acid) in animal milk

Номер: CN116064502A
Автор: LI ZHIPENG, JIANG HANSI, ZHENG YALIN, LIU QINGYOU, CUI KUIQING, REN HONGHE, WANG SHUWAN, LI LING, GONG HUICHAO
Принадлежит:

The invention relates to a method for extracting creamy fat ball RNA (Ribonucleic Acid) in animal milk. The steps are simplified into pretreatment, splitting decomposition, layering, alcohol precipitation, washing, drying and dissolving. By adding the step of pretreating the milk sample, mixing the DEPC stock solution with the milk sample and quickly freezing the milk sample with the liquid nitrogen, the DEPC can react with a plurality of nuclease groups, so that the activity of RNA enzyme is destroyed, the temperature of the liquid nitrogen is about-196 DEG C, the milk sample can instantly reach a low-temperature state, degradation of endogenous RNA enzyme in the milk to milk fat balls is inhibited, and the milk fat balls can be quickly frozen. Therefore, the effect of protecting the cream ball RNA is achieved. According to the method, the defects of an existing method are overcome, the extraction time and the extraction steps are optimized, RNA degradation is inhibited to the maximum ...

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Номер записи: 2
27-05-2015 дата публикации

Method for realizing gene knockout in porcine embryonic cells by using CRISPR/Cas system

Номер: CN104651399A
Автор: LIU QINGYOU, SHI DESHUN, SU XIAOPING, LIU SHUAI, ZHU PENG, FENG WANYOU, CUI KUIQING
Принадлежит:

The invention discloses a method for realizing gene knockout in porcine embryonic cells by using a CRISPR/Cas system. The method comprises the following steps: constructing a Cas9 eukaryotic expression vector; constructing a gRNA expression vector; inserting a target sequence of a desired gene in the gRNA expression vector; linearizing the Cas9 eukaryotic expression vector and the gRNA expression vector in which the desired gene is inserted by using a restriction endonuclease, carrying out in vitro transcription by taking linearized plasmids as templates so as to respectively obtain mRNA of hSpCas9 and sgRNA of gRNA2 #, and finally, injecting the mRNA of hSpCas9 and the sgRNA of gRNA2 # into cytoplasms of porcine IVF embryos by a microscope, thereby realizing gene knockout. The method disclosed by the invention has the advantages of more sensibility and effectiveness, low cost, wider applicable scope.

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Номер записи: 3