柴油机发电机组控制器（8）

Low-temperature preservation solution for stichopus japonicus sperms, application and preservation method of stichopus japonicus sperms

The invention belongs to the technical field of sperm preservation, and particularly relates to stichopus japonicus sperm low-temperature preservation liquid, application and a stichopus japonicus sperm preservation method. The low-temperature preservation solution is prepared by taking an isotonic buffer solution as a basic solution and then adding a mixed solution of fetal calf serum and mycillin. According to the method for combining the low-temperature short-term preservation technology and the ultralow-temperature freezing long-term preservation technology, the stichopus japonicus sperms can keep more than 70% of vitality within 4 days and keep more than 50% of vitality within 10 days under the preservation condition of 4 DEG C, and the frozen stichopus japonicus sperms with the vitality of more than 35% can be obtained by performing ultralow-temperature freezing preservation within 4 days of short-term preservation; the sperm short-term preservation operation in the method is simple ...

Apostichopus japonicus whole genome 30K liquid phase breeding chip and application thereof

The invention relates to the technical field of genomics, molecular biology, bioinformatics and whole genome selective breeding, in particular to an apostichopus japonicus whole genome 30K liquid phase breeding chip based on a target area genome sequence liquid phase capture accurate positioning sequencing typing technology (cGPS) and application of the apostichopus japonicus whole genome 30K liquid phase breeding chip. The SNP molecular marker combination is any combination of 1 to 30781 SNP sites. The chip can be applied to stichopus japonicus genetic diversity evaluation, germplasm resource and genetic relationship identification, important economic character whole genome association analysis and whole genome selective breeding; the method has the advantages of high throughput, high interval coverage rate, high site detection rate, high flexibility and the like, and provides powerful tool support for apostichopus japonicus molecular breeding.

INTERNAL BATTERY HOLDER

The present invention relates to the technical field of electric bike accessories. Provided is an internal battery holder capable of solving a problem of inconvenient installation of existing battery holders. The internal battery holder is provided at a bottom bracket shell of an electric bike and comprises a main body (1). A first accommodation recess (3) is arranged inside the main body (1) for receiving a unit cell module. A central shaft hole (4) is arranged at the main body (1). The central shaft hole (4) extends through the main body (1) and has a shape and a size which match a shape and a size of a central shaft provided at the bottom bracket shell. A battery and a controller (17) are integrated into the internal battery holder. The central shaft hole (4) arranged at the main body (1) matches a central shaft provided at a bottom bracket shell of an electric bike, such that an electric bike adopting the internal battery holder has a more compact and pleasant appearance. The internal ...

Gene molecular marker related to growth traits of stichopus japonicus and application of gene molecular marker

The invention belongs to the field of molecular marker-assisted breeding, and particularly relates to a molecular marker combination related to growth traits of stichopus japonicus and application of the molecular marker combination. The stichopus japonicus sif2 gene molecular marker is screened out, a stichopus japonicus molecular marker-assisted breeding method is established, and a gene marker and a technical method are provided for genetic improvement of growth traits of stichopus japonicus. The molecular marker is located in an exon region of the stichopus japonicus sif2 gene. The molecular marker obviously related to the growth traits of the stichopus japonicus is obtained by screening through a Sanger sequencing method and comprises the following sites: Tgt at the 1538th site from a transcription start site; c mutation is carried out, and Tgt at the 1574th site; c mutation, and Tgt at the 29283rd site; c mutation. During application, one or more molecular markers are used for breeding ...