Method capable of improving synchronization estrus rate and pregnancy rate of embryo transplantation receptor cattle
The invention belongs to the field of animal husbandry and veterinary medicine and embryo engineering. The method capable of improving a synchronization estrus rate and a pregnancy rate of an embryo transplantation receptor cattle includes conducting intramuscular injection of vitamin ADE of 10ml into a receptor kettle regardless of period any day, placing progestational hormone pessary for 9-12 days, conducting intramuscular injection of pregnant mare serum gonactotropin of 1,000 units one day before taking the pessary, and conducting intramuscular injection of cloprostenol of 0.5mg before taking the pessary. The receptor kettle ruts after taking the pessary for 2-3 days, and conducting chorionic gonadotropin of 2,000 units or luteinizing hormone releasing hormone A3 for injection of 25 mcg. The receptor kettle which is processed, ruts and suffers from light metritis is filled with anti-inflammation medicine on a uterus on the same day, purifying the uterus, simultaneously conducting intramuscular injection of vitamin ADE of 10ml, so that intrauterine environment of the receptor kettle is beneficial to pregnancy. Embryo transplantation is conducted on a seventh-seventh and half day of estrus. The receptor kettle without solid corpus luteum is injected with progesterone of 100 units or luteinizing hormone of 25 mcg. Estrus rate is larger than 90%, and pregnancy rate is more than 60%. 1. With the induction of the estrus and transplantation vitamin ADE 10 ml vitamin ADE in order to ensure that the needs of the cattle, to maintain reproductive system function oestrus and impregnates. 2. The selected receptor ox in any one-day vaginal suppository (CIDE) hormone the laying aside is pregnant , 9-12 days out. 3. The former for removing thrombus from 1 day intramascular is pregnant the horse blood serum pmsg (PMSG) 1000 units. 4. For removing thrombus from the forefront of the alcohol sodium intermuscular injection chlorine (PG-CL)0.5 mg, or clindamycin palace note. 5. after taking the hitch, 2-3 day in oestrus medium-term intramascular human chorionic gonadotropic hormone (HCG) 2000 units or acted 3 (LHRH-A3) of 25 micrograms, with process integrity of the oestrus performance, promote ovulation and mature follicular development, forming energetic solid corpus luteum. 6. Metaxalone to a slight heat of the oestrus cattle, the day of uterine perfusion antibiotic or antibiotic pharmaceutical anti-inflammatory, purifying the uterus, the receptor ox the intrauterine environment more conducive to the embryo survival, implantation, pregnancy. 7. In oestrus section 7-7.5 days embryo transplantation. 8. When not enough to transplant a solid elongated the intramascular progesterone 100 units or luteotropin myog 25 micrograms, play a role for tocolysis miscarriage.