Multi-fluorogenic quantitative PCR (polymerase chain reaction) method for detecting duck hepatitis A virus (DHAV) and identifying DHAV-A and DHAV-C and kit

The invention discloses a multi-fluorogenic quantitative PCR (polymerase chain reaction) method for detecting a duck hepatitis A virus (DHAV) and identifying DHAV-A and DHAV-C and a kit, and belongs to the technical field of biological detection. Aiming at a DHAV3D area, the invention designs a pair of conservative amplification primers (SEQIDNO:1 and SEQIDNO:2) and a conservative probe (SEQIDNO:5) to detect different gene types of DHAVs. A pair of specific primers (SEQIDNO:3 and SEQIDNO:4) and two specific probes (SEQIDNO:6 and SEQIDNO:7) designed by aiming at DHAV5'UTR are used to identify DHAV-A and DHAV-C. The method has excellent specificity, repeatability and sensitivity, is convenient, simple and quick in operations, is used for identifying and diagnosing the infection of DHAV as well as DHAV-A and DHAV-C in scientific research and clinical application, and is also used for the epidemiological investigation of DHAV.

Indirect ELISA method and kit for detecting serum 3-type duck hepatitis virus a antibody

The invention discloses an indirect ELISA method and kit for detecting serum 3-type duck hepatitis virus a antibody and belongs to the technical field of serum antibody detection. The indirect ELISA method includes that serum 3-type duck hepatitis virus VP1 protein is utilized as an envelope antigen with the peridium quantity as 0.1-1mug per hole, HRP-mice anti-duck IgY is utilized as the HRP with the use concentration as 0.2-2mug/mL, and the coloration time is 10 minutes. The kit comprises a VP1 protein antigen envelope board, the HRP-mice anti-duck IgY, sample diluent, a scrubbing solution, TMB solutions A and B and a stop solution. The method and the kit can be used for detecting the serum 3-type duck hepatitis virus a antibody in duck serum and duck egg yolk, and the serum does not have cross reaction with positive serum of other viruses. Compared with a traditional neutral reaction detection method, the method has the advantage of being convenient and accurate.

Fire water-supply equipment control cabinet with emergency starting fire water pump mechanical device

The invention discloses a control cabinet of fire control water supply equipment provided with an emergency starting fire control pump mechanism, which belongs to the technical field of control cabinet manufacturing. The control cabinet consists of a main loop consisting of a double power switching circuitry and low voltage appliances; wherein, the control cabinet is provided with an operation handle, a bayonet pin, a linked bar, an offsetting spring, a rotary switch, a cross bar, a short bar and a main contactor; wherein, the handle is located at one end of the linked bar; the bayonet pin is arranged at a side beam of a cabinet body of the control cabinet; the linked bar is intertwined with the offsetting spring; the rotary switch is arranged at the end of the linked bar far from the handle; the linked bar is connected with the cross bar perpendicularly and axially; the main contactor is arranged in the center of the control cabinet; the short bar is arranged in the middle place, where ...

Fire-fighting water supply equipment controlling cabinet with automatic moistureproof dehumidifier

The invention discloses a fire-fighting water supply device control cabinet provided with an automatic moistureproof dehumidifier, belonging to the control cabinet manufacturing technical field. The fire-fighting water supply device control cabinet comprises a main circuit which consists of a double power switching circuit and a low-voltage apparatus and also comprises a detector which is arranged on the top of the control cabinet and a dehumidifying heater which is arranged at the bottom of the control cabinet, wherein, the detector is connected with the dehumidifying heater by adoption of an antiflaming twisted pair. The environment of a fire service pump house tends to be moist and the relative humidity generally possibly exceeds 90 percent and reaches the degree of moisture condensation, thereby the normal operation of the water supply device control cabinet in the pump house is severely threatened. In order to overcome the influence of the condition on the fire water supply device ...

Method and special reagent kit for detecting five zoonosis virus

The invention discloses a method for diagnosing five varieties of disease virus infected by both human beings and animals and a special kit thereof. The kit comprises a primer set A used for diagnosing foot and mouth disease virus: the DNA indicated by the sequence 1 in the sequence table, and the DNA indicated by the sequence 2 in the sequence table. The method of using the kit for diagnosing viruses has the advantages of high specificity and reliable results. When using a plurality of varieties of primers for diagnosing simultaneously, the diagnosis of the plurality of varieties of viruses in an identical PCR reaction can be achieved, the diagnosis efficiency is high, time and reagent are saved greatly and cost is reduced; therefore, the method has convenient, rapid and sensitive advantages, has significance for enhancing the monitoring of disease virus infected by both human beings and animals and for preventing the propagation of the disease virus infected by both human beings andanimals ...

Colloidal gold immunochromatography test paper for antibodies of pseudorabies viruses of gE, gB and gD and preparation method

The invention discloses a colloidal gold immunochromatography test paper for antibodies of pseudorabies viruses of gE, gB and gD and a preparation method. The test paper comprises a sample absorption area, a gold mark probe area, an immobilization antigen and antibody area, a water absorption area and a supporting plate, and the sample absorption area, the gold mark probe area, the immobilization antigen and antibody area and the water absorption area are laid on the supporting plate and are partially overlapped in sequence. The gold mark probe area is covered with gold mark probes which are mouse anti-swine IgG antibodies marked by colloidal gold. The immobilization antigen and antibody area is provided with a detection line T1 covered with pseudorabies virus gE protein, a detection line T2 covered with pseudorabies virus gB protein, a detection line T3 covered with pseudorabies virus gD protein and a control line C covered with goat anti-mouse IgG antibodies. The test paper is fast in ...

Fire-fighting water supply equipment controlling cabinet with emergency key for opening cabinet door

The invention discloses a fire-fighting water supply device control cabinet provided with a cabinet door emergency opening key device, belonging to the control cabinet manufacturing technical field. The fire-fighting water supply device control cabinet has a structure of arranging a voltmeter, an ammeter, a plurality of indicator lamps and buttons on a front panel of a control cabinet body, and is also provided with the cabinet door emergency opening key device on an obvious position on the front panel of the control cabinet body. When a fire event occurs, if a cabinet door is required to be opened immediately due to failure in the cabinet and a cabinet door key can not be found immediately for a while, an authorized person can immediately break up transparent glass on the device, take out a key, open a cabinet door and repair the failure so as to guarantee that a fire pump can be started normally.

Model mixed precision reasoning method, device and equipment and storage medium

The invention discloses a model mixing inference method, device and equipment and a storage medium, and the method comprises the steps: inputting an input sample into a deep learning model in a chip, carrying out the calculation of the input sample through a calculation node in the chip, and obtaining a float32 type target result; obtaining a segment list of the model, and adjusting the precision selection parameter of each segment according to a precision mixing result and a target result of the model for each segment under a preset precision selection parameter; and inputting a target precision selection parameter of each calculation node in each segment as a control signal into a control node, selecting a matched precision calculation branch through the control node in the chip, and completing mixed precision reasoning through the calculation node according to the precision calculation branch. According to the technical scheme provided by the embodiment of the invention, the mixed-precision ...

Method and special reagent kit for detecting five zoonosis virus

The invention discloses a method for diagnosing five varieties of disease virus infected by both human beings and animals and a special kit thereof. The kit comprises a primer set A used for diagnosing foot and mouth disease virus: the DNA indicated by the sequence 1 in the sequence table, and the DNA indicated by the sequence 2 in the sequence table. The method of using the kit for diagnosing viruses has the advantages of high specificity and reliable results. When using a plurality of varieties of primers for diagnosing simultaneously, the diagnosis of the plurality of varieties of viruses in an identical PCR reaction can be achieved, the diagnosis efficiency is high, time and reagent are saved greatly and cost is reduced; therefore, the method has convenient, rapid and sensitive advantages, has significance for enhancing the monitoring of disease virus infected by both human beings and animals and for preventing the propagation of the disease virus infected by both human beings and animals ...

Fire water-supply equipment control cabinet with emergency starting fire water pump mechanical device

The invention discloses a control cabinet of fire control water supply equipment provided with an emergency starting fire control pump mechanism, which belongs to the technical field of control cabinet manufacturing. The control cabinet consists of a main loop consisting of a double power switching circuitry and low voltage appliances; wherein, the control cabinet is provided with an operation handle, a bayonet pin, a linked bar, an offsetting spring, a rotary switch, a cross bar, a short bar and a main contactor; wherein, the handle is located at one end of the linked bar; the bayonet pin isarranged at a side beam of a cabinet body of the control cabinet; the linked bar is intertwined with the offsetting spring; the rotary switch is arranged at the end of the linked bar far from the handle; the linked bar is connected with the cross bar perpendicularly and axially; the main contactor is arranged in the center of the control cabinet; the short bar is arranged in the middle place, wherethe ...

Digital multifunctional special fire water supply device controller

The invention relates to a digital multifunctional special fire water supply device controller, comprising a programmable logic controller, a pressure sensor and a PID adjustor; wherein, the programmable logic controller is provided with a steering instruction receive port, a fire pump control port and a frequency conversion control port, the steering instruction receive port is used to receive digital steering instructions, the fire pump control port is used to be connected to a fire pump main control loop to control startup and stop of the fire pump, and the frequency conversion control port is used to be connected to a frequency converter to control operation of the frequency converter; the pressure sensor is used to be connected to a fire water supply network to detect network pressure and generate simulated pressure signals; the PID adjustor is used to process the simulated pressure signals and then transmit the signals to the frequency converter to control output frequency of the ...

Digital multifunctional special fire water supply device controller

The invention relates to a digital multifunctional special fire water supply device controller, comprising a programmable logic controller, a pressure sensor and a PID adjustor; wherein, the programmable logic controller is provided with a steering instruction receive port, a fire pump control port and a frequency conversion control port, the steering instruction receive port is used to receive digital steering instructions, the fire pump control port is used to be connected to a fire pump main control loop to control startup and stop of the fire pump, and the frequency conversion control port is used to be connected to a frequency converter to control operation of the frequency converter; the pressure sensor is used to be connected to a fire water supply network to detect network pressure and generate simulated pressure signals; the PID adjustor is used to process the simulated pressure signals and then transmit the signals to the frequency converter to control output frequency of the ...

Fire-fighting water supply equipment controlling cabinet with pressure controlling device

The invention discloses a fire-fighting water supply device control cabinet provided with a pressure controller device, belonging to the control cabinet manufacturing technical field. The fire-fighting water supply device control cabinet comprises a main circuit which consists of a double power switching circuit and a low-voltage apparatus, wherein, the pressure controller is arranged inside the control cabinet, and a thin water pipe is connected to the pressure controller and passes through the control cabinet. The pressure controller for starting a fire pump is arranged in the structure of the double power switching circuit and the low-voltage main circuit of the cabinet, overcomes the defects that contacts of a general pressure controller are easy to go rusty and moldy when the general pressure controller is arranged under the moist and dusty unfavorable environment of the fire pump room, and guarantees that the pressure controller can operate normally and continuously.

Gene chip for detecting zoonosis virus and application thereof

The invention discloses a gene chip for detecting a zoonosis virus and application thereof. The gene chip comprises virus detection probes, and is characterized in that the virus detection probes comprises probes the nucleotide sequences of which are respectively sequences 1-150 in table 2, and probes the nucleotide sequences of which are respectively reverse complement sequences of the sequences 1-150. The optimum conditions that the gene chip is hybridized with nucleic acid of the virus are as follows: the chip is closed with 0.25g/100ml NaBH4 solution and is hybridized with nucleic acid in hybridization solution containing 50% (volume percent) formamide hydridization solution at the temperature of 51 DEG C for 2 hours; and under the conditions, the gene chip provided by the invention has the advantages of better sensitivity and better detection specificity.

Flavivirus-detecting gene chip probe and gene chip detection method

The invention provides a group of flavivirus-detecting gene chip probes and a general detection method. The probes include a Tick borne encephalitis probe, a Denguo virus probe and a Japanese encephalitis probe. A general primer sequence of flaviviruses capable of specific reverse transcription is designed, and flavivirus specific primers are utilized to perform reverse transcription for guiding the synthesis of viral genome so as to reduce interference in host cell gene components to the fullest extent and improve the specificity and accuracy of detection results. The invention can be used for the gene amplification of all flaviviruses and the gene chip detection of Tick borne encephalitis, Denguo virus and Japanese encephalitis.

Gene chip for detecting 6 viruses of zoonosis-borne diseases and application thereof

The invention discloses a gene chip for viruses of zoonosis-borne diseases and application thereof. The gene chip for the viruses of the zoonosis-borne diseases is a gene chip for detecting at least one virus in six viruses of hepatitis E virus, Nipah virus, rabies virus, Rift Valley fever virus, foot and mouth disease virus and Vesicular stomatitis virus, and is fixed with a hepatitis E virus detection probe, a Nipah virus detection probe, a rabies virus detection probe, a Rift Valley fever virus detection probe, a foot and mouth disease virus detection probe and a Vesicular stomatitis virus detection probe. The invention also discloses a method for detecting the Rift Valley fever virus, the Nipah virus, the foot and mouth disease virus, the Vesicular stomatitis virus, the hepatitis E virus and/or the rabies virus. The invention can stably and specifically implement generic detection of the six viruses of the zoonosis-borne diseases, has the advantages of simpleness, convenience, quickness ...

Flavivirus-detecting gene chip probe and gene chip detection method

Porcine pseudorabies virus gE IgM antibody colloidal gold immunochromatograohic assay test strip as well as preparation method and application thereof

The invention discloses a porcine pseudorabies virus gE IgM antibody colloidal gold immunochromatograohic assay test strip as well as a preparation method and application thereof. The test strip comprises a sample absorption region, a colloidal gold labeled probe region, a solidifying antibody region, a water absorption region, a bottom plate and a clamping shell, wherein the sample absorption region, the colloidal gold labeled probe region, the solidifying antibody region and the water absorption region are sequentially bonded on the bottom plate and are interlapped in the clamping shell; the sample absorption region is coated with purified porcine pseudorabies virus gE protein; the colloidal gold labeled probe region is coated with colloidal gold labeled porcine pseudorabies virus gE protein monoclonal antibody; the solidifying antibody region is sequentially provided with a detection line T coated with mouse anti-pig IgM monoclonal antibody and a control line C coated with goat anti-mouse ...