24-03-2022 дата публикации
Номер: US20220088186A1
Принадлежит:
Disclosed is an SamRNA vaccine, including a recombinant viral vector which includes: i) a viral gene replication complex including nucleotide sequences encoding viral gene replication-related proteins nsP1, nsP2, nsP3, and nsP4; and ii) a nucleotide sequence encoding at least one antigen. According to the SamRNA vaccine of the present invention, in addition to that a promoter of a modified adenoviral vector itself can transcribe an antigen gene to form mRNA, the viral gene replication-related proteins nsP1-4 use RNA as a template to synthesize a large amount of mRNAs, and the immune effect of a target antigen is greatly improved. 1. An SamRNA vaccine , comprising a recombinant viral vector which comprises: i) a viral gene replication complex comprising nucleotide sequences encoding viral gene replication-related proteins nsP1 , nsP2 , nsP3 , and nsP4; and ii) a nucleotide sequence encoding at least one antigen.2. The SamRNA vaccine according to claim 1 , wherein the recombinant viral vector is a recombinant adenovirus claim 1 , a chimpanzee adenovirus claim 1 , a recombinant vesicular stomatitis virus claim 1 , a recombinant poxvirus claim 1 , a recombinant dengue virus claim 1 , a recombinant Kunjin virus claim 1 , a recombinant sendai virus claim 1 , or a recombinant canine distemper virus.3. The SamRNA vaccine according to claim 1 , wherein the antigen causes an immune response against bacteria claim 1 , viruses claim 1 , fungi or parasites.4. The SamRNA vaccine according to claim 3 , wherein the antigen is a human herpes zoster virus gE protein claim 3 , a rotavirus VP4 or VP7 claim 3 , an HPV-L1 protein claim 3 , or an Ebola virus gP protein.5. The SamRNA vaccine according to claim 1 , wherein the antigen is a tumor-specific antigen claim 1 , and is selected from NY-ESO-1 claim 1 , SSX2 claim 1 , SCP1 claim 1 , RAGE claim 1 , BAGE claim 1 , GAGE claim 1 , MAGE family polypeptides claim 1 , p53 claim 1 , p21/Ras claim 1 , CDK4 claim 1 , MUM1 claim 1 , caspase-8 ...
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