15-03-2012 дата публикации
Номер: US20120064047A1
Принадлежит:
The invention provides isolated stem cells of non-embryonic origin that can be maintained in culture in the undifferentiated state or differentiated to form cells of multiple tissue types. Also provided are methods of isolation and culture, as well as therapeutic uses for the isolated cells in cardiac indications. 111-. (canceled)12. A method of providing cardiac muscle to a subject in need thereof , said method comprising administering to the subject , in an amount effective to provide new cardiac muscle , isolated expanded human multipotent non-ES , non-germ , and non-embryonic germ cells that express telomerase , have undergone 10-40 cell doublings in culture , and have a normal karyotype , wherein the existing cardiac muscle is damaged by disease or injury.13. The method of wherein the non-embryonic claim 12 , non-germ cells further express one or more of oct4 claim 12 , rex-1 claim 12 , rox-1 claim 12 , or sox-2.14. A method of providing cardiac muscle to a subject in need thereof claim 12 , said method comprising administering to the subject claim 12 , in an amount effective to provide new cardiac muscle claim 12 , isolated expanded human multipotent non-ES claim 12 , non-germ claim 12 , and non-embryonic germ cells that express oct4 claim 12 , have undergone 10-40 cell doublings in culture claim 12 , and have a normal karyotype claim 12 , wherein the existing cardiac muscle is damaged by disease or injury.15. The method of wherein the non-embryonic claim 14 , non-germ cells further express one or more of telomerase claim 14 , rex-1 claim 14 , rox-1 claim 14 , or sox-2.16. A method of providing cardiac muscle to a subject in need thereof claim 14 , said method comprising administering to the subject claim 14 , in an amount effective to provide new cardiac muscle claim 14 , isolated expanded human multipotent non-ES claim 14 , non-germ claim 14 , and non-embryonic germ cells that have undergone 40 cell doublings in culture and have a normal karyotype claim 14 , ...
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