28-03-2019 дата публикации
Номер: US20190090750A1
Described herein is a multiplex platform that uses ultrasmall nanoparticles (e.g., C dots and C′ dots) to graphically differentiate specific nerves (e.g., sensory nerves vs. motor nerves) for nerve transplants and other surgeries. Also described herein is a multiplex platform that uses ultrasmall nanoparticles (e.g., C dots and C′ dots) to graphically differentiate between different types of lymph nodes and/or lymphatic pathways, e.g., to safely and effectively perform vascularized lymph node transplantation in the treatment of lymphedema. Also described herein is a multiplex platform that uses ultrasmall nanoparticles (e.g., C dots and C′ dots) to graphically differentiate parathyroid tissue. 1. A method comprising:administering two or more different probe species each comprising a spectrally differentiable fluorescent reporter to a lymphatic system; anddirecting excitation light into the lymph nodes, thereby exciting the fluorescent reporters having spectrally distinguishable emission wavelengths.2. The method of claim 1 , wherein the administering comprises intravenously administering two or more different probe species.3. The method of claim 1 , wherein the two or more different probe species comprise nanoparticles.4. The method of claim 1 , wherein at least a first probe is administered to a tumor site and at least a second probe is administered to an extremity that would be potentially affected by lymphedema.5. The method of claim 4 , wherein the tumor site comprises a member selected from the group consisting of a breast claim 4 , a trunk claim 4 , an abdomen claim 4 , a pelvis claim 4 , and a thoracic cavity.6. The method of claim 4 , wherein the extremity comprises a member selected from the group consisting of an upper limb and a lower limb.7. The method of claim 1 , wherein the excitation light comprises two or more wavelengths claim 1 , thereby exciting the different fluorescent reporters.8. The method of claim 1 , comprising identifying an appropriate ...
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