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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 52596. Отображено 100.
30-05-2019 дата публикации

Газовый хроматограф

Номер: RU0000189684U1
Автор: Анатолий Константинович Давыденков, Леонид Владимирович Илясов
Принадлежит: Анатолий Константинович Давыденков, Леонид Владимирович Илясов

Полезная модель относится к аналитической технике, а именно к хроматографическим анализаторам состава газообразных и жидких сред. Газовый хроматограф содержит стабилизатор расхода газа-носителя, снабженный выходным штуцером, последовательное соединение дозатора, снабженного входным штуцером, испарителя жидких сред, хроматографической колонки и газового детектора, у которого выход испарителя и вход хроматографической колонки, а также выход этой колонки и вход газового детектора соединены капиллярными трубками, переменный дроссель с входным и выходным штуцерами и тройник, причем переменный дроссель через входной штуцер подключен к капиллярной трубке, соединяющей испаритель с входом хроматографической колонки, а выходной штуцер этого дросселя через тройник подключен к капиллярной трубке, соединяющей выход хроматографической колонки с входом газового детектора, хроматограф также содержит два дополнительных дросселя и дополнительный тройник, при этом вход дополнительного тройника подключен к выходному штуцеру стабилизатора расхода газа-носителя, а его два выхода соединены с входами дополнительных переменных дросселей, выход одного из которых подключен к тройнику, а выход другого - к входному штуцеру дозатора. Техническим результатом является увеличение точности газового хроматографического анализа состава газообразных и жидких многокомпонентных сред. 2 ил. РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 189 684 U1 (51) МПК G01N 30/02 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ПОЛЕЗНОЙ МОДЕЛИ К ПАТЕНТУ (52) СПК G01N 30/02 (2019.02) (21)(22) Заявка: 2019107953, 20.03.2019 (24) Дата начала отсчета срока действия патента: (73) Патентообладатель(и): Илясов Леонид Владимирович (RU), Давыденков Анатолий Константинович (RU) Дата регистрации: 30.05.2019 (56) Список документов, цитированных в отчете о поиске: RU 84123 U1, 27.06.2009. CN (45) Опубликовано: 30.05.2019 Бюл. № 16 1 8 9 6 8 4 R U (54) ГАЗОВЫЙ ХРОМАТОГРАФ (57) Реферат: Полезная модель относится ...

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Номер записи: 1
21-07-2020 дата публикации

Устройство для калибровки газоаналитического оборудования в полевых условиях

Номер: RU0000198626U1
Автор: Владислав Андреевич Кузнецов, Марина Владимировна Волкодаева
Принадлежит: федеральное государственное бюджетное образовательное учреждение высшего образования "Санкт-Петербургский горный университет"

Полезная модель относится к области технического и метрологического обеспечения мониторинга атмосферного воздуха и может быть использована для контроля качества получаемых первичных данных с газоаналитического оборудования.Устройство для калибровки газоаналитического оборудования в полевых условиях, содержащее калибровочный пакет прямоугольной формы. Корпус выполнен в форме прямоугольного параллелепипеда, внутри которого установлена перегородка, которая образует два отсека, внутри каждого из отсеков так же установлены перегородки, в отсеках установлены не менее чем по два калибровочных пакета в каждом, на передней грани корпуса выполнено сквозное отверстие, в которое установлены внешний кран, который расположен с внешней стороны корпуса, и внутренний кран - с внутренней стороны корпуса, на кранах закреплены с возможностью съема вентили регулировки порционной подачи/отбора калибровочной газовой смеси, к внутреннему крану с обеих сторон подключены магистрали, которые установлены над перегородкой внутри отсеков, на магистрали у задней грани корпуса установлен переходник, в нижней части корпуса каждого калибровочного пакета установлен фитинг, который соединен с магистралью, на противоположной стороне, по диагонали от него, установлен фитинг с заглушкой, по периметру корпуса калибровочного пакета выполнены внутренний и внешний шов путем спаивания. 6 ил. РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 198 626 U1 (51) МПК G01N 30/04 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ПОЛЕЗНОЙ МОДЕЛИ К ПАТЕНТУ (52) СПК G01N 30/04 (2020.02) (21)(22) Заявка: 2020109526, 03.03.2020 (24) Дата начала отсчета срока действия патента: 21.07.2020 Приоритет(ы): (22) Дата подачи заявки: 03.03.2020 (45) Опубликовано: 21.07.2020 Бюл. № 21 U 1 1 9 8 6 2 6 R U (54) УСТРОЙСТВО ДЛЯ КАЛИБРОВКИ ГАЗОАНАЛИТИЧЕСКОГО ОБОРУДОВАНИЯ В ПОЛЕВЫХ УСЛОВИЯХ (57) Реферат: Полезная модель относится к области установлены внешний кран, который расположен технического и метрологического ...

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Номер записи: 2
03-05-2012 дата публикации

Sample preparation for gas analysis using inductive heating

Номер: US20120103062A1
Автор: Carl Chang, Gregor Hsiao
Принадлежит: Picarro Inc

Improved gas analysis for non-gaseous samples is provided by placing the sample in direct contact with an inductive heating element, followed by inductively heating the heating element to provide gas for analysis. Disposable sample vials including such a heating element can be employed, or a sample tube including an inductive heating element can be configured to mate to the input gas line of a gas analysis system.

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Номер записи: 3
24-05-2012 дата публикации

Methods and materials for performing hydrophobic interaction chromatography

Номер: US20120125843A1
Автор: Edouard S. P. Bouvier, Hua Yang
Принадлежит: Waters Technologies Corp

A method for performing hydrophobic interaction chromatography includes providing at least one wall defining a chamber having an inlet and an exit, and a stationary phase disposed within the chamber. The stationary phase comprises particles or monolith having a hydrophobic surface and a hydrophilic ligand. The method also includes loading a sample onto the stationary phase in the chamber and flowing the sample over the stationary phase. The sample is separated into one or more compositions by hydrophobic interaction between the stationary phase and the one or more compositions.

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Номер записи: 4
14-06-2012 дата публикации

Sample Collection System And Method

Номер: US20120144897A1
Автор: Robert S. Johnson, Stephen Scheufele
Принадлежит: Horizon Technology Inc

An apparatus or method for removing water and concentrating an analyte in solution, wherein the concentrated analyte sample is delivered directly to a vial, such as an autosampler vial that is capable of use in a gas chromatography autosampler.

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Номер записи: 5
14-06-2012 дата публикации

Field flow fractionator with controllable cross flow along its length

Номер: US20120148460A1
Автор: Philip J. Wyatt
Принадлежит: Wyatt Technology LLC

A field flow fractionator to separate particles contained within an injected sample aliquot is described. As required, said fractionator may be used to capture, for subsequent removal, specific predefined classes of such particles. Based upon the cross flow or asymmetric flow field flow fractionators, the fractionator disclosed contains means to vary the applied transverse flows at a plurality of locations along the length of its separating channel. One embodiment utilizes a plurality of separated compartments, each lying below a distinct and corresponding membrane supporting permeable frit segment, are provided individual means to control the localized flow through the membrane section thereabove. A corresponding concentric compartment implementation achieves the same type of compartmentalized cross flow when integrated with a hollow fiber fractionator.

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Номер записи: 6
02-08-2012 дата публикации

Devices and method for enrichment and alteration of cells and other particles

Номер: US20120196273A1
Автор: Bruce L. Carvalho, Lotien Richard Huang, Mehmet Toner, Paul Vernucci, Ravi Kapur, Thomas A. Barber, Zihua Wang
Принадлежит: Individual

The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.

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Номер записи: 7
08-11-2012 дата публикации

Vaporisation injector

Номер: US20120280061A1
Автор: Eric Phillips, Paolo Magni, Stefano Pelagatti
Принадлежит: Thermo Fisher Scientific SpA

The invention relates to a vaporization injector for a gas chromatograph, said injector comprising a structure ( 11 ) mounted in a detachable manner on the gas chromatograph body and including the sample introduction means, the vaporization chamber and pneumatic connections for feeding the carrier gas to the vaporization chamber and to the septum purge means, as well as pneumatic connections for evacuating the splitted sample and carrier gases.

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Номер записи: 8
15-11-2012 дата публикации

Method for determining equivalent thermal conditions between liquid chromatography systems

Номер: US20120285223A1
Автор: Peyton C. Beals, Richard W. Andrews
Принадлежит: Waters Technologies Corp

Described is a method of transferring a chromatographic method between liquid chromatography (LC) systems. The method is based on a determination of an isoretention temperature at which two solutes co-elute. The method enables separations to be performed using different LC systems with reproducible and equivalent results. For example, the method allows for a chromatography method developed for HPLC to be more readily transferred to a UPLC system and for a chromatography method developed for a UPLC system to be more readily transferred to a HPLC system. The method addresses LC systems having column ovens of different design in which the internal column temperatures are not equal although the operating temperatures of the column ovens may be accurately controlled to equal values. The retention behavior and resolution of different LC systems is caused to be substantially the same so that equivalent separation results are obtained.

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Номер записи: 9
15-11-2012 дата публикации

Inhibiting binding of fgf23 to the binary fgfr-klotho complex for the treatment of hypophosphatemia

Номер: US20120288886A1
Автор: Anna V. ELISEENKOVA, Moosa Mohammadi, Regina GOETZ
Принадлежит: New York University NYU

The present invention is directed to a method of treating hypophosphatemia in a subject. The present invention is also directed to a method of screening for compounds suitable for treatment of hypophosphatemia associated with elevated or normal FGF23. This method involves providing FGF23, FGFR-Klotho complex, and one or more candidate compounds. The FGF23, the FGFR-Klotho complex, and the candidate compounds are combined under conditions effective for the FGF23 and the binary FGFR-Klotho complex to form a ternary complex if present by themselves. This method also involves identifying the candidate compounds, which prevent formation of the complex as being potentially suitable in treating hypophosphatemic conditions associated with elevated or normal FGF23. A method of screening the specificity of compounds which prevent formation of the FGF23-Klotho-FGFR complex is also disclosed.

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Номер записи: 10
29-11-2012 дата публикации

Microchemical nanofactories

Номер: US20120298037A1
Автор: Brian Kevin Paul, Chih-Hung Chang, Vincent Thomas Remcho
Принадлежит: Oregon State Board of Higher Education, State University

Embodiments of an apparatus, system, and method for chemical synthesis and/or analysis are disclosed. One embodiment of a disclosed apparatus comprises a laminated, microfluidic structure defining a reactor and a separator. Such apparatuses, or portions thereof, generally have dimensions ranging from about 1 micrometer to about 100 micrometers. To implement synthetic processes, disclosed embodiments of the apparatus generally include at least one unit operation, such as a mixer, a valve, a separator, a detector, and combinations thereof. Individual apparatuses may be coupled both in series and in parallel to form a system for making chemical compounds. An individual apparatus or a system also can be used in combination with known devices and processes.

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Номер записи: 11
03-01-2013 дата публикации

Self-sealing sample compartment for a liquid chromatography system

Номер: US20130004388A1
Автор: Daniel J. Gagne, James E. Usowicz, Joshua A. Shreve
Принадлежит: Waters Technologies Corp

Described is a self-sealing thermal enclosure. In various embodiments, the self-sealing thermal enclosure includes an enclosure having a wall with an opening. The enclosure is configured to surround a temperature-controlled environment. The self-sealing thermal enclosure also includes a porous seal disposed adjacent to the wall at the opening. The porous seal is compressible and is fabricated from an open cell foam material. When the porous seal has absorbed a fluid such as a condensate, the temperature-controlled environment is sealed from an ambient environment such that the flow of air into or out of the enclosure is substantially reduced or eliminated.

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Номер записи: 12
14-03-2013 дата публикации

METHOD FOR DETERMINING METHANOL CONTENT IN CRUDE OILS

Номер: US20130061658A1
Автор: DiSanzo Frank P., MORITA Hisao, Shi Eric S., SHIBASAKI Masato, TAKAYAMA Norio, YAMAMOTO Shigeharu
Принадлежит: EXXONMOBIL RESEARCH AND ENGINEERING COMPANY

A test method for the determination of methanol in crude oils to levels as low as 0.5 ppm is disclosed. The method includes extracting methanol into a water phase from a test sample of the crude oil forming a test sample extract. The method further includes extracting methanol into a water phase from a reference sample of the crude oil forming a reference sample extract, wherein the reference sample having a predetermined amount of methanol added thereto. The method further includes measuring the methanol content in the test sample extract and the methanol content in the reference sample extract. The method also includes determining the methanol content of the crude oil based upon the methanol content in the test sample extract and the methanol content in the reference sample extract. 1. A method of determining methanol content in a crude oil , comprising:extracting methanol into a water phase from a test sample of the crude oil forming a test sample extract;extracting methanol into a water phase from a reference sample of the crude oil forming a reference sample extract, wherein the reference sample having a predetermined amount of methanol added thereto;measuring the methanol content in the test sample extract;measuring the methanol content in the reference sample extract; anddetermining the methanol content of the crude oil based upon the methanol content in the test sample extract and the methanol content in the reference sample extract.2. The method according to claim 1 , wherein extracting methanol into a water phase from a test sample of the crude oil includes mixing a specified amount of the test sample with a mixture of water and a solvent.3. The method according to claim 2 , wherein extracting methanol into a water phase from a test sample of the crude oil further includes separating the crude oil from the water claim 2 , wherein the methanol is contained in the water.4. The method according to claim 3 , further comprising:performing an additional ...

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Номер записи: 13
28-03-2013 дата публикации

Sampling device and method of use thereof

Номер: US20130074581A1
Автор: II Howard William Ward, Joel Michael Hawkins, Peter Alfred Blacklin, Wayne Fowler, JR.
Принадлежит: Mettler Toledo AG

An sampling device for capturing a material sample and a method of using said device to process said material sample in situ within the device. Embodiments of the invention may be disposed as elongate probes having extendable sample capture elements. A sample capture element of such a device may include a sample capture pocket located near a distal end thereof for capturing and trapping a sample of material. The sample capture pocket may be provided with a port for receiving material therein and a port for expelling material therefrom. These ports may be placed in communication with corresponding material transfer channels extending through the sample capture element to allow for the in situ processing of a material sample, and the subsequent discharge of the sample to an analyzer or another downstream location.

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Номер записи: 14
28-03-2013 дата публикации

Liquid-transport and analytical test device

Номер: US20130078147A1
Автор: Darius Haeusler, Eric Jallerat, Karl Pflanz, Markus Hollas, Uwe Andag
Принадлежит: SARTORIUS STEDIM BIOTECH GMBH

A liquid-transport device has a liquid-tight support ( 12 ) on which there is applied a start zone ( 24; 24′ ) for applying transport liquid to be transported and a target zone ( 26, 28; 26 a - e ) into which the transport liquid is to be transported and also a conduction zone that extends between the start zone ( 24, 24′ ) and the target zone ( 26, 28; 26 a - e ) and that has a microporous transport layer ( 14 ) in which the transport liquid flows by capillary force from the start zone ( 24; 24′ ) to the target zone ( 26, 28; 26 a - e ). The conduction zone has a multiplicity of open flow channels separated from one another by microporous bridges having open-pored side walls.

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Номер записи: 15
11-04-2013 дата публикации

SEPARATION/DETECTION COLUMN AND KIT THEREOF

Номер: US20130089468A1
Автор: Ikeda Isamu, Minoda Toshiharu
Принадлежит:

Disclosed is a separation/detection column that can detect a component separated by a separating agent having optical responsivity which is the same as that of a component in a sample. The separation/detection column is configured by filling one end of a tube having ultraviolet transparency, with a first filler that has ultraviolet responsivity and a separation ability to separate the component in the sample, and by filling the other end of the tube with a second filler that has ultraviolet responsivity which is different from that of the first filler and does not have the separation ability via a spacer constituted by quartz wool, and moreover by inserting an end cap constituted by quartz wool into the other end of the tube 1. A separation/detection column that has columnar or tubular first and second stationary phases having liquid permeability , with ends of the first and second stationary phases being connected to each other so that liquid is permeable , and a moving phase being permeated in the axis direction of the first and second stationary phases , whereinthe first stationary phase has a separation ability to separate a component in a sample, and optical responsivity to an ultraviolet ray or a coloring reagent, which is the same as the optical responsivity of this component, andthe second stationary phase has an optical responsivity which is different from that of the component.2. The separation/detection column according to claim 1 , wherein the second stationary phase does not have the separation ability.3. The separation/detection column according to claim 1 , wherein each of the first and second stationary phases is formed of a packed layer formed by particulate filler in a column tube claim 1 , a columnar body having liquid permeability claim 1 , or a separating agent layer having liquid permeability claim 1 , which is formed on a peripheral surface of a columnar or tubular support body not having liquid permeability.4. The separation/detection column ...

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Номер записи: 16
18-04-2013 дата публикации

LIQUID MIXING DEVICE AND LIQUID CHROMATOGRAPH

Номер: US20130091933A1
Автор: Akieda Daisuke, Kaji Hironori, Nagaoka Yoshihiro, Tsukada Nobuhiro
Принадлежит:

A liquid mixing device that decreases a concentration non-uniformity in the flow direction of a mobile phase and a liquid chromatograph that uses the liquid mixing device are provided. The liquid mixing device is configured to include a flow channel unit that is made from an introduction channel, a branch portion that is positioned in a downstream of the introduction channel, multiple branched flow channels that branch from the branch portion, a junction portion in which the multiple branched flow channels join together, and a discharge channel of the downstream of the junction portion. The multiple branched flow channels are different in terms of any one of, or several of width, depth, and length that are associated with an external shape, and a structure filling the inside of the flow channel and thus the times for the liquid to pass through the branched flow channels are different from each other. 1. A liquid mixing device comprising:an introduction channel through which liquid is introduced;a branch portion that is positioned in a downstream of the introduction channel;a plurality of branched flow channels that branch from the branch portion;a junction portion in which the plurality of branched flow channels join together; anda discharge channel of the downstream of the junction portion,wherein in such a manner that the discharge channel of an upstream side flow channel unit of a plurality of flow channel units in which times for the liquid to pass through the plurality of branched flow channels are different from each other is the introduction channel of a downstream side flow channel unit, the discharge channel and the introduction channel are connected to each other, and in such a manner as to differentiate between differences between the times for the liquid to pass through the plurality of branchnt channels, the discharge channel and the introduction channel are connected to each other.2. The liquid mixing device according to claim 1 ,wherein one of the ...

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Номер записи: 17
18-04-2013 дата публикации

BLOOD COLLECTION MODULE FOR MEASURING ALCOHOL CONCENTRATION

Номер: US20130091934A1
Автор: PARK Ik Hyun, Park Kwang Hee
Принадлежит: ELECHEM CO., LTD.

A blood collection module includes a blood collection container that is coupled with a blood alcohol concentration detection device including an alcohol detection sensor having a detection probe that inhales an alcohol gas, and on one surface of which an inserting portion that is inserted by a detection probe of a blood alcohol concentration detection device is formed and on an outer surface of which blood inlet holes through which blood flows in are formed; and an absorption member that is provided in the blood collection container, to thus absorb examinee's blood that is introduced through the blood inlet holes, in which an alcohol gas generated from the blood absorbed by the absorption member is introduced into the alcohol detection sensor through the detection probe. 1. A blood collection module for measuring blood alcohol concentration , the blood collection module comprising:a blood collection container which includes an inserting portion against which a detection probe of a blood alcohol concentration detection device having an alcohol detection sensor and the detection probe is inserted, and blood inlet holes through which blood is introduced, andan absorption member that is provided in the blood collection container to thus absorb examinee's blood that is introduced through the blood inlet holes,wherein an alcohol gas generated from the blood absorbed by the absorption member is introduced into the alcohol detection sensor through the detection probe.2. The blood collection module according to claim 1 , wherein the blood collection container further comprises a container cover that covers an upper surface of the blood collection container claim 1 , wherein the inserting portion is formed on the container cover in the form of a plurality of slits or score lines.3. The blood collection module according to claim 1 , wherein a handle that is laterally extended from the container cover is provided for the container cover and a tear-off portion is formed in the ...

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Номер записи: 18
18-04-2013 дата публикации

LIQUID CHROMATOGRAPH AND PUMP UNIT FOR LIQUID CHROMATOGRAPH

Номер: US20130091935A1
Автор: Akieda Daisuke
Принадлежит: HITACHI HIGH-TECHNOLOGIES CORPORATION

In a liquid chromatograph and a pump unit for liquid chromatograph whereby a sample is analyzed while changing the mixing ratio of multiple kinds of solvents, mixing of the solvents can be accelerated without adding any novel device such as a mixer. The pump unit for liquid chromatograph has a configuration which comprises cylinders for introducing a multiple kinds of solvents thereinto and plungers that are reciprocated so as to introduce the solvents and discharge the same for sending, wherein a plurality of channels are formed in the cylinders for mixing the solvents. 1. A liquid chromatograph for introducing a sample while changing a mixing ratio of multiple kinds of solvents , and separating the sample by a separation column to detect components , comprising:a pump unit configured to send the multiple kinds of the solvents to the separation column, whereinsaid pump unit has cylinders into which said multiple kinds of the solvents are introduced, plungers that are reciprocated so as to introduce said multiple kinds of the solvents and discharge the same for sending, and a plurality of channels in which said multiple kinds of the solvents are mixed.2. The liquid chromatograph according to claim 1 , whereinsaid plurality of channels are provided on a side where said multiple kinds of the solvents are discharged from said cylinders.3. The liquid chromatograph according to claim 2 , whereina confluent portion is provided on rear stream sides of said plurality of channels.4. The liquid chromatograph according to claim 2 , whereina filter is provided on rear stream sides of said plurality of channels.5. The liquid chromatograph according to claim 1 , whereinsaid plurality of channels are provided on a side where said multiple kinds of the solvents are introduced into said cylinders.6. The liquid chromatograph according to claim 5 , whereina filter is provided on front sides of said plurality of channels.7. A pump unit for liquid chromatograph claim 5 , the pump unit ...

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Номер записи: 19
25-04-2013 дата публикации

BIOMARKER FOR RENAL FUNCTION IN PATIENTS WITH TYPE 2 DIABETES

Номер: US20130101578A1
Автор: Chia Kee Seng, Holthofer Harry, Ng Peng Keat Daniel, Tai Bee Choo
Принадлежит: NATIONAL UNIVERSITY OF SINGAPORE

The invention provides kits and methods for the diagnosis, prognosis, and treatment of reduced kidney function in patients, such as diabetic patients. The methods include a step of detecting one or more nephrin degradation products in a sample from the subject, such as a urine sample. 1. A method of detecting type 2 diabetes-related reduced renal function in a mammalian subject comprising detecting one or more nephrin degradation products in a urine sample from the subject , wherein the nephrin degradation product has an apparent molecular weight of about 25 kDa , 50 kDa , 60 kDa and/or 75 kDa , and detection of the nephrin degradation product(s) indicates the presence of type 2 diabetes-related reduced renal function in the subject.2. The method of claim 1 , wherein the subject is a human.3. The method of claim 1 , wherein the subject is normoalbuminuric.4. (canceled)5. The method of claim 1 , wherein the nephrin degradation products has an apparent molecular weight of about 25 kDa.6. The method of claim 1 , wherein the nephrin degradation products is detected using an antibody.7. The method of claim 6 , wherein the antibody is a monoclonal antibody.8. The method of claim 1 , wherein the mammal exhibits a decline in eGFR of at least 1.0 ml/min/1.73 m.9. The method of claim 1 , wherein the subject with type 2 diabetes has been diagnosed as diabetic for a year or less.10. The method of claim 1 , wherein the nephrin degradation products is detected by ELISA claim 1 , Western Blotting claim 1 , RIA claim 1 , HPLC claim 1 , SPR claim 1 , nucleic acid or protein aptamers claim 1 , SAT claim 1 , peptide sequencing claim 1 , and/or MS/MS.11. The method of claim 1 , wherein the nephrin degradation products is detected in a cell free fraction of the urine sample.12. The method of claim 1 , further comprising administering a suitable treatment for reduced renal function claim 1 , wherein the treatment is one or more antagonists of the renin-angiotensin system (RAS).13. A ...

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Номер записи: 20
25-04-2013 дата публикации

INTERNAL STANDARDS AND METHODS FOR USE IN QUANTITATIVELY MEASURING ANALYTES IN A SAMPLE

Номер: US20130102478A1
Автор: Amoura Mohamed
Принадлежит: WATERS TECHNOLOGIES CORPORATION

The invention provides methods for quantitatively analyzing a plurality of analytes in a sample. Also described are general and specific internal standards useful in such analysis. In particular embodiments, these standards are described as useful in liquid chromatography/mass spectroscopy systems, which are also described herein. Moreover, in certain embodiments, the quantification methods of the present invention are useful in increasing the precision and/or accuracy of multiple analyte quantification for analytes contained in a single sample mixture using known analyte derivatives simultaneously analyzed, and compared to the unknown analytes. 2. The method of claim 1 , wherein the analyte derivative standard has been formed by derivatizing an analyte standard with a second derivatizing agent claim 1 , wherein the second derivatizing agent is 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate claim 1 , phenylisothiocyanate claim 1 , or a functional derivative thereof claim 1 , which has been labeled with an isotope.3. The method of claim 1 , further comprising the step of derivatizing a known concentration of analyte standards with a second derivatizing agent to form the known concentration of a plurality of analyte derivative standards claim 1 , wherein the second derivatizing agent is 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate claim 1 , phenylisothiocyanate claim 1 , or a functional derivative thereof claim 1 , which has been labeled with an isotope.4. The method of claim 1 , wherein the detection of the analyte derivative and the corresponding analyte derivative standard is measured as a 1:1 response ratio.5. The method of claim 2 , wherein the isotope is a radioactive isotope or a stable isotope.6. The method of claim 5 , wherein the isotope is a stable isotope selected from the group consisting of C claim 5 , N claim 5 , and H.7. The method of claim 3 , wherein the isotope is a radioactive isotope or a stable isotope.8. The method of claim 7 , wherein the ...

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Номер записи: 21
02-05-2013 дата публикации

PUMP FOR LIQUID CHROMATOGRAPH, AND LIQUID CHROMATOGRAPH

Номер: US20130104631A1
Автор: Akieda Daisuke, Hiraku Kenji, Ishii Kimihiko, Kaji Hironori, Tokuo Kenichiro, Yasuhara Naoya
Принадлежит:

A pump controller causes a first plunger pump and a second plunger pump connected in series or in parallel to perform intake and compression actions alternately at substantially constant cycles, sets a pressurizing chamber of one of the plunger pumps to a state of a higher pressure than the pressurizing chamber of the other plunger pump, and performs flow rate control by adjusting lift amounts of the first plunger and the second plunger. Thus, it is possible to provide a pump for liquid chromatograph, and a liquid chromatograph, which are capable of reducing pulsations even when an ejection flow rate is changed. 1. A pump for liquid chromatograph characterized in that the pump comprises:a first plunger pump including, a first pressurizing chamber communicating with a first intake channel and a first ejection channel, and a first plunger configured to reciprocate inside the first pressurizing chamber;a second plunger pump including, a second pressurizing chamber communicating with a second intake channel and a second ejection channel, and a second plunger configured to reciprocate inside the second pressurizing chamber;a connection flow channel connecting the first plunger pump and the second plunger pump in series or in parallel;at least one electric motor configured to generate rotative power;a power transmission mechanism configured to convert the rotative power of the electric motor into linear reciprocating power and to transmit the power to the first plunger and the second plunger;a motor driver configured to control the electric motor;at least one pressure detecting means provided in the first pressurizing chamber, the second pressure chamber, or a channel on a downstream side of any of the pressuring chambers; anda pump controller configured to read a measurement value of the pressure detecting means, to provide the motor driver with an instruction value, to cause the first plunger pump and the second plunger pump to perform intake and compression actions ...

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Номер записи: 22
16-05-2013 дата публикации

Clocked Blowing Away of a Contaminated Gas Cloud

Номер: US20130118230A1
Автор: Aichinger Karl, Dunzinger Bernhard, Straubinger Hans-Jurgen
Принадлежит: KRONES AG

The disclosure relates generally to a method for testing containers for foreign substances, wherein a standard gas is blown into a container to be tested, at least a part of the test gas escaping from the container is tested by a measuring device, and the part of the test gas remaining outside the measuring device is removed from the measuring area in a clocked manner, e.g. by blowing it away or sucking it off. 1. A method for testing containers for foreign substances , the method comprising that a standard gas is blown into a container to be tested , that at least a part of the test gas escaping from the container is tested by a measuring device , and that the part of the test gas remaining outside the measuring device is removed from the measuring area in a clocked manner.2. A method according to claim 1 , the method comprising that the part of the test gas remaining outside the measuring device is blown away from the measuring area in a clocked manner under a high pressure if the tested container (B) is no longer located in the measuring area (R).3. A method according to claim 1 , wherein the part of the test gas remaining outside the measuring device is blown away and/or sucked off from the measuring area in a clocked manner only if a contamination of the tested container was detected.4. A method according to claim 1 , wherein at least a part of the test gas escaping from the container is tested by a measuring device chromatographically.5. A method according to claim 1 , wherein the blown-away part of the test gas remaining outside the measuring device is conducted to an outlet and/or is sucked off.6. A method according to claim 1 , wherein the standard gas comprises air claim 1 , an inert gas claim 1 , a noble gas claim 1 , a noble gas mixture claim 1 , or a combination of the aforementioned gases.7. A method according to claim 1 , wherein the part of the test gas remaining outside the measuring device is blown away with ambient air or technically purified air/ ...

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Номер записи: 23
23-05-2013 дата публикации

METHOD AND APPARATUS FOR INSPECTING A GAS SAMPLE

Номер: US20130125620A1
Автор: Linenberg Amos, Ovadia Yuval
Принадлежит: S.T.I. Security Technology Integration Ltd.

An apparatus for detecting a presence of at least one analyte in a gas sample. The apparatus comprises a pump for drawing a gas sample from an ambient air, a passage having first and second ends, a chamber connected to the first end and containing a concentrating element for collecting at least one analyte from the gas sample, a chromatographic separator connected to a second end of the passage, and a gas source for streaming a carrier gas via the chamber to transfer the at least one analyte toward at least one chemical detector, via the chromatographic separator, in a first direction. The pump draws the gas sample via the chamber in a second direction and the first and second directions are substantially opposing to one another. 1. A method for inspecting a gas sample , comprising:a) drawing a gas sample through a chamber having a concentration element so as to allow at least one analyte from said gas sample to be bonded thereto;b) extracting impurities from said chamber by streaming a carrier gas toward a first outlet therethrough;c) heating said concentration element to release said at least one bonded analyte;d) streaming said carrier gas via said chamber so as to carry said at least one released analyte toward a second outlet, via a chromatographic separator and a detection unit; ande) reading an output of said detection unit for detecting at least one of the presence, the absence and the concentration of said at least one analyte.2. The method of claim 1 , wherein said streaming is performed during a period of less than 10 seconds.3. The method of claim 1 , wherein said b)-e) are performed in less than 20 seconds.4. The method of claim 1 , wherein said b)-e) are performed in less than 10 seconds.5. The method of claim 1 , further comprising 0 cooling said concentration element in less than 10 seconds.6. The method of claim 5 , wherein said a)-f) are iteratively performed in detection cycles of less than 1 minute.7. The method of claim 1 , wherein said heating ...

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Номер записи: 24
30-05-2013 дата публикации

Method and Arrangement for Gas Chromatographic Analysis of a Gas Sample

Номер: US20130133403A1
Автор: Gellert Udo, Probst Frank
Принадлежит: SIEMENS AKTIENGESELLSCHAFT

The invention relates to a gas sample to be analyzed, wherein said sample is guided by means of a carrier gas through a separator unit having a downstream thermal conductivity detector providing a chromatogram having peaks for different analytes as a measurement signal. When using a thermal conductivity detector having a heated gold thread coated with a parylene F, hydrogen is used as a carrier gas, and a peak for the analyte hydrogen sulfide is generated by differentiating the chromatogram at the location of said analyte. The invention permits unlimited use of hydrogen as a carrier gas, even if the analyte is oxygen. 15.-. (canceled)6. A method for gas chromatographic analysis of a gas sample , comprising:conveying the gas sample by a carrier gas comprising hydrogen through a separating device having a downstream thermal conductivity detector including an electrically heated gold filament coated with parylene F;delivering, from the thermal conductivity detector, a chromatogram having peaks for different analytes as a measurement signal; anddifferentiating the chromatogram at a position of an analyte of the different analytes to generate a peak for a hydrogen sulfide analyte.7. The method as claimed in claim 6 , wherein the measurement signal is differentiated by an RC component formed by connecting the thermal conductivity detector by at least one capacitor to an input of an evaluation device.8. The method as claimed in claim 6 , wherein an oxygen analyte is detected in the carrier gas hydrogen.9. The method as claimed in claim 7 , wherein an oxygen analyte is detected in the carrier gas hydrogen.10. An arrangement for gas chromatographic analysis of a gas sample claim 7 , comprising:a separating device having a downstream thermal conductivity detector including an electrically heated gold filament coated with parylene F, the gas sample being conveyed by a carrier gas through the separating device, the thermal conductivity detector delivering a chromatogram having ...

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Номер записи: 25
30-05-2013 дата публикации

PRESSURE SENSING AND FLOW CONTROL IN DIFFUSION-BONDED PLANAR DEVICES FOR FLUID CHROMATOGRAPHY

Номер: US20130133760A1
Автор: Bunner Bernard, Dourdeville Theodore A., Gerhardt Geoff C.
Принадлежит: WATERS TECHNOLOGIES CORPORATION

Flow through pressure sensors for use in fluid chromatography systems include a planar device formed from diffusion bonding of a plurality of metallic sheets and at least one sensing element. The planar device has a top surface, a bottom surface and a flow through channel. A diaphragm formed from a portion of one of the top or bottom surfaces is located adjacent to a sensing region of the flow through channel and is attached to the sensing element. The diaphragm is sized to deflect a distance in response to fluid pressure in the sensing region, which has an internal volume of less than about 25 microliters. The diaphragm and attached sensing element form a pressure sensor that measures strain or deflection of the diaphragm to calculate a pressure within the sensing region. 1. A flow through pressure sensor for use in a system for chromatographic separation , the flow through pressure sensor able to withstand pressures of at least about 40 megapascals and comprising:a planar device formed from a plurality of metallic parts attached by diffusion bonding, the planar device having a top surface, a bottom surface, and at least one flow through channel disposed between the top and bottom surfaces; anda sensing element located on a diaphragm formed from a portion of at least one of the top surface or bottom surface of the planar device, the diaphragm bounding one face of a first sensing region of the at least one flow through channel and being sized to deflect a distance in response to fluid pressure in the first sensing region, the first sensing region having an internal volume of about 25 microliters or less.2. The flow through pressure sensor according to claim 1 , wherein the sensing element measures mechanical strain of the diaphragm for use in calculating fluid pressure in the first sensing region of the at least one flow through channel.3. The flow through pressure sensor according to claim 1 , wherein the sensing element measures deflection of the diaphragm for use ...

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Номер записи: 26
30-05-2013 дата публикации

DEVICE AND METHODS FOR PERFORMING SIZE EXCLUSION CHROMATOGRAPHY

Номер: US20130135610A1
Автор: Bouvier Edouard S.P., Neue Lynda, Walter Thomas H., Wyndham Kevin D.
Принадлежит: WATERS TECHNOLOGIES CORPORATION

The present invention is directed to a device and a method for performing size exclusion chromatography. Embodiments of the present invention feature devices and methods for size exclusion chromatography at normal high performance liquid chromatography or ultra performance liquid chromatography pressures and above using small particles. 2. The device for performing size exclusion chromatography according to claim 1 , wherein W and Q occupy free valences of the core composition claim 1 , X claim 1 , or on the surface of said core composition3. The device for performing size exclusion chromatography according to claim 1 , wherein W and Q are selected to form a surface composition on the surface of said core composition claim 1 , and X forms a block polymer or group of block polymers.4. The device for performing size exclusion chromatography according to claim 1 , wherein the stationary phase comprises particles.5. The device for performing size exclusion chromatography according to claim 4 , wherein the particles of the stationary phase material have diameters with a mean size distribution of 0.4-3.0 microns or of 1.0-3.0 microns.6. (canceled)7. The device for performing size exclusion chromatography according to claim 1 , wherein the stationary phase comprises a monolith.8. The device for performing size exclusion chromatography according to claim 7 , wherein the monolith of the stationary phase material exhibits the chromatographic efficiency and permeability of a particle bed packed with particles having a mean size distribution of 0.4-3.0 microns or of 1.0-3.0 microns.9. (canceled)10. The device for performing size exclusion chromatography according to claim 1 , wherein the particles or the monolith of the stationary phase material have a pore volume of 0.8 to 1.7 cm/g claim 1 , of 1.0 to 1.5 cm/g or of 1.1 to 1.5 cm/g.11. (canceled)12. (canceled)13. The device for performing size exclusion chromatography according to claim 1 , wherein the chamber is capable of ...

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Номер записи: 27
30-05-2013 дата публикации

AIRCRAFT SCREENING DEVICE AND METHOD

Номер: US20130137183A1
Автор: Nacson Sabatino
Принадлежит: Teknoscan Systems, Inc.

A method of screening aircraft passengers and/or cargo comprising loading an aircraft with passengers, cargo or both, circulating air within the aircraft so that the air is in contact with the passengers and/or cargo, expelling some of the circulated air from the aircraft, diverting a portion of the air being expelled from the aircraft through a chemical filter configured to retained evidence of a target substance, and analyzing the chemical filter to detect the presence of a target substance within the aircraft. 1. A method of screening aircraft passengers or cargo comprising:loading an aircraft with passengers, cargo or both;circulating air within the aircraft so that the air is in contact with the passengers or cargo;expelling some of the circulated air from the aircraft;diverting a portion of the air being expelled from the aircraft through a chemical filter configured to retain evidence of a target substance; andanalyzing the chemical filter to detect the presence of the target substance within the aircraft.2. The method of wherein the method comprises loading the aircraft with passengers and cargo claim 1 , the cargo comprising an air freight container claim 1 , and the method further comprises:drawing a sample from the container using suction before the container is loaded into the aircraft;passing the sample through a chemical filter configured to retain evidence of a target substance in the sample; andanalyzing the chemical filter to detect the presence of the target substance within the container.3. The method of claim 2 , further comprising:drawing a sample from a passenger's clothing or hand luggage using suction prior to the passenger boarding the aircraft;passing the sample through a chemical filter configured to retain evidence of a target substance, andanalyzing the chemical filter to detect the presence of the target substance on the passenger's clothing or hand luggage.4. The method of wherein air is circulated by activating an onboard air ...

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Номер записи: 28
06-06-2013 дата публикации

VOLATILE ORGANIC COMPOUNDS FOR DETECTING CELL DYSPLASIA AND GENETIC ALTERATIONS ASSOCIATED WITH LUNG CANCER

Номер: US20130143247A1
Автор: Haick Hossam, Peled Nir
Принадлежит: TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LTD.

The present invention provides methods of identifying a genetic abnormality such as mutation in EGFR or KRAS or ALK which is associated with the management of lung cancer or diagnosing, prognosing or monitoring the treatment of pre-cancerous conditions of the lung, such as bronchial dysplasia or atypical alveolar hyperplasia (AAH), through the detection of at least one volatile organic compound indicative of these states. 1. A method of identifying a genetic alteration selected from a mutation in EGFR , a mutation in KRAS , an ALK-ELM4 translocation and CMET amplification , wherein the genetic alteration is associated with lung cancer , the method comprising the steps of:a) obtaining a sample from a test subject;b) determining the level of at least one volatile organic compound in the test sample; andc) comparing the level of the at least one volatile organic compound from the test sample with the level of said at least one volatile organic compound in a negative control sample, whereby a significantly different level of said at least one volatile organic compound in the test sample as compared to the level of said compound in the negative control sample is indicative of the presence of said genetic alteration.2. The method according to claim 1 , wherein the at least one volatile organic compound is selected from the group consisting of 4-methyl-1-heptanol claim 1 , acetic acid octyl ester claim 1 , decane claim 1 , 3-methyl-decane claim 1 , octanal claim 1 , pentadecanenitrile claim 1 , and tetradecene; or selected from the group consisting of 4-methyl-1-heptanol claim 1 , 6-methyl-1-heptanol claim 1 , 2-ethyl-1-hexanol claim 1 , acetic acid octyl ester claim 1 , benzaldehyde claim 1 , decance claim 1 , 3-methyl-dodecance claim 1 , tetrahydrofuran claim 1 , isopropyl myristate claim 1 , octanal claim 1 , pentadecanenitrile claim 1 , 2 claim 1 ,2 claim 1 ,4-trimethyl-pentanenitrile claim 1 , 2 claim 1 ,2 claim 1 ,4-trimethyl-3-carboxyisopropyl-isobutyl ester ...

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Номер записи: 29
27-06-2013 дата публикации

Method of measuring the electroosmotic transport coefficient of a proton exchange membrane and device for implementing such a method

Номер: US20130166222A1
Автор: Arnaud Morin, Zhe Peng
Принадлежит: Commissariat a lEnergie Atomique et aux Energies Alternatives CEA

A method of determining the electroosmotic transport coefficient of a proton exchange membrane, the method including creating a stream of hydrated hydrogen on either side of the membrane which is permanently controlled so that the relative humidity is almost identical on each side of the membrane at any point, thereby making it possible to minimize any back diffusion into the membrane. Furthermore, the method includes estimating the back diffusion flux into the membrane from the rate of return to equilibrium of the relative humidity starting from the moment when the current is cut off.

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Номер записи: 30
04-07-2013 дата публикации

MORPHOLOGY ENGINEERING OF CONDUCTIVE METALLIC NANOPARTICLES CAPPED WITH AN ORGANIC COATING

Номер: US20130171733A1
Автор: GLIKSMAN Sadi, Haick Hossam, SEGEV-BAR Meital, SHUSTER Gregory
Принадлежит: TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LTD.

The present invention is directed to a sensor having continuous and discontinuous regions of conductive metallic nanoparticles capped with an organic coating which enables the detection of volatile organic compounds and/or water vapor. 1. A sensor for detecting an analyte selected from a volatile organic compound , water vapor and combinations thereof , the sensor comprising continuous and discontinuous regions of conductive metallic nanoparticles capped with an organic coating , wherein the continuous and discontinuous regions differentially detect water vapor and volatile organic compounds.2. The sensor according to claim 1 , wherein the continuous regions exhibit a positive response upon exposure to volatile organic compounds and to water vapor claim 1 , and the discontinuous regions exhibit a positive response upon exposure to volatile organic compounds and a negative response upon exposure to water vapor.3. The sensor according to claim 1 , wherein the discontinuous regions comprise voids ranging in size from about 10 nm to about 500 nm.4. The sensor according to configured in a form selected from the group consisting of a capacitive sensor claim 1 , a resistive sensor claim 1 , a chemiresistive sensor claim 1 , an impedance sensor claim 1 , and a field effect transistor sensor.5. The sensor according to which is a chemiresistor comprising a film comprising continuous and discontinuous regions of conductive metallic nanoparticles capped with an organic coating formed on a substrate.6. The sensor according to claim 5 , wherein the substrate is a rigid substrate or a flexible substrate.7. The sensor according to claim 5 , wherein the substrate is selected from the group consisting of metals claim 5 , insulators claim 5 , semiconductors claim 5 , semimetals claim 5 , polymers claim 5 , and combinations thereof.8. The sensor according to claim 7 , wherein the substrate a polymer selected from the group consisting of polyimide claim 7 , polyamide claim 7 , polyimine ...

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Номер записи: 31
04-07-2013 дата публикации

REAGENT COMPOSITION FOR NUCLEIC ACID CHROMATOGRAPHY OR IMMUNOCHROMATOGRAPHY, METHOD FOR MEASUREMENT BY NUCLEIC ACID CHROMATOGRAPHY OR IMMUNOCHROMATOGRAPHY, AND KIT FOR MEASUREMENT BY NUCLEIC ACID CHROMATOGRAPHY OR IMMUNOCHROMATOGRAPHY

Номер: US20130171740A1
Автор: Sakakibara Yuhiro
Принадлежит: TANAKA KIKINZOKU KOGYO K.K.

A reagent composition for nucleic acid chromatography or immunochromatography which includes a water-soluble polymer having a weight average molecular weight of 8,000 or more, a salt of a divalent or trivalent metal, a nonionic surfactant, and an aprotic water-soluble organic compound. The reagent composition is capable of determining an analyte accurately and rapidly even when it has a low concentration in a measurement by nucleic acid chromatography or immunochromatography, by reducing the binding of components other than the analyte through a non-specific reaction and enhancing the dispersion capability of the analyte to improve the developability on a chromatography carrier and to promote a specific reaction. 1. A reagent composition for nucleic acid chromatography or immunochromatography comprising a water-soluble polymer having a weight average molecular weight of 8 ,000 or more , a salt of a divalent or trivalent metal , a nonionic surfactant , and an aprotic water-soluble organic compound.2. The reagent composition according to claim 1 , wherein the water-soluble polymer having a weight average molecular weight of 8 claim 1 ,000 or more is one or more kinds selected from the group consisting of polyalkylene glycols claim 1 , celluloses claim 1 , vinyl-based polymers claim 1 , amide-based polymers claim 1 , and a polyanion.3. The reagent composition according to claim 2 , wherein the polyanion is a polysaccharide anion.4. The reagent composition according to claim 3 , wherein the polysaccharide anion is one or more kinds selected from the group consisting of dextran sulfate claim 3 , heparan sulfate claim 3 , chondroitin sulfate claim 3 , dermatan sulfate claim 3 , keratan sulfate claim 3 , hyaluronic acid claim 3 , heparin claim 3 , and salts thereof.5. The reagent composition according to claim 1 , wherein the aprotic water-soluble organic compound is one or more kinds selected from the group consisting of sulfoxides and N claim 1 ,N′-dialkylamides.6. The ...

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Номер записи: 32
04-07-2013 дата публикации

PROGNOSIS AND TREATMENT OF BREAST CANCER

Номер: US20130172430A1
Автор: Lisanti Michael P., Sotgia Federica
Принадлежит:

Methods of prognosis and monitoring of breast cancer include determining the level of one or more of the markers comprising asymmetric dimethyl arginine (ADMA), beta-hydroxybutyrate (BHB) and microRNA-31 (miR-31) in patient samples. An increased level is correlated with poor prognosis. Breast cancer is treated by administration of one or more inhibitors of ADMA and/or BHB. 1. A prognostic method for breast cancer in a subject comprising:(a) determining the level of at least one of the following in a test sample from a subject: (i) asymmetric dimethyl arginine; (ii) beta-hydroxybutyrate; and (iii) miR-31; andb) comparing the level of at least one of (i) asymmetric dimethyl arginine, (ii) beta-hydroxybutyrate, and (iii) miR-31 in said test sample to the level of in a control sample, wherein an elevated level of at least one of (i) asymmetric dimethyl arginine, (ii) beta-hydroxybutyrate, and (iii) miR-31 in said test sample relative to the level in said control sample is a prognostic indicator of the course of breast cancer disease in said subject.2. A method of monitoring the progression of breast cancer in a subject , said method comprising:(a) obtaining a first sample from a subject at a first time point and a second sample from said subject at a second time point;(b) determining the level of at least one of the following in said first and second samples: (i) asymmetric dimethyl arginine; (ii) beta-hydroxybutyrate; and (iii) miR-31; and(c) comparing the level of said at least one of (i) asymmetric dimethyl arginine, (ii) beta-hydroxybutyrate, and (iii) miR-31 in said first sample to the level in said second sample, wherein an elevated level of at least one of (i) asymmetric dimethyl arginine, (ii) beta-hydroxybutyrate, and (iii) miR-31 in said second sample relative to the level in said first sample is an indication that the cancer has progressed in said subject.3. The method of or wherein the level of asymmetric dimethyl arginine is determined.4. The method of or ...

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Номер записи: 33
11-07-2013 дата публикации

Evaluating heparin preparations for pharmaceutical use

Номер: US20130177992A1
Автор: David Schrier, Megan Sucato, Nur Sibel Gunay, Stephen Smith, Zachary Shriver
Принадлежит: Momenta Pharmaceuticals Inc

The disclosure features methods of analyzing preparations of heparin, and materials derived from heparin using strong anion exchange high performance liquid chromatography (SAX-HPLC).

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Номер записи: 34
25-07-2013 дата публикации

Micro-scale passive vapor preconcentrator/injector

Номер: US20130186174A1
Автор: Edward T. Zellers, Jung Hwan Seo, Katsuo Kurabayashi, Sun Kyu KIM
Принадлежит: University of Michigan

A passive and reusable preconcentrator/injector device for measuring gas-phase analytes and methods of use. The device includes an upper plate defining an array of micro-scale diffusion channels and a lower plate secured to the upper plate. The lower plate defines a cavity for a reusable collection material in fluid communication with the micro-scale diffusion channels. An integral heating unit is provided adjacent the lower plate and configured for heating the cavity. A loading port may be included for introducing the reusable collection material into the cavity. An inlet port and an outlet port are provided, both in fluid communication with the cavity. The device may include a fluidic manifold system comprising a plurality of conduits disposed between the adsorbent cavity and the outlet port.

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Номер записи: 35
25-07-2013 дата публикации

NANOFLOW DETECTOR CELL

Номер: US20130186187A1
Автор: Ahmed Ahmed-Yacine Badjah Hadj, ALOTHMAN Zeid Abdullah
Принадлежит: KING SAUD UNIVERSITY

A nanoflow detector cell comprises a nanoflow detection cell template defming a sample channel transverse template and a reference channel transverse template, generally parallel to the sample channel, and spaced apart from the sample channel. Clear capillary tubing extends through the sample channel, defming a sample chamber, a portion of the capillary tubing extends out of each end of the sample channel, and is shaped to the template. 1. A nanoflow detection cell comprising:a nanoflow detection cell template comprising:a sample channel transverse template; anda reference channel transverse template, generally parallel to the sample channel and spaced apart from the sample channel; and clear capillary sample tubing extending through the sample channel defining a sample chamber, a portion of the capillary sample tubing extending out of each end of the sample channel and shaped to the template.2. The nanoflow detection cell of wherein the capillary sample tubing has an outside diameter of one millimeter or less claim 1 , and an internal diameter in a range of 25 to 500 micrometers.3. The nanoflow detection cell of wherein the capillary sample tubing has a volume in the range between three nanoliters and four microliters.4. The nanoflow detection cell of wherein the template is generally parallelepiped and the portion of the capillary sample tubing extending out of each end of the sample channel is shaped along a side of the template.5. The nanoflow detection cell of wherein the template further comprises a flange portion and the portion of the capillary sample tubing extending out of each end of the sample channel and shaped to the template extends through orifices in the flange to provide an inlet and an outlet for the nanoflow detection cell.6. The nanoflow detection cell of wherein the sample channel corresponds to a light source of the detector at one end and a sample photo-detector of the detector at the other end claim 1 , whereby claim 1 , the capillary sample ...

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Номер записи: 36
01-08-2013 дата публикации

GAS CHROMATOGRAPHY - INVERSE GAS CHROMATOGRAPHY COMBINED ANALYSIS DEVICE

Номер: US20130192340A1
Автор: Liu Baizhan, WANG Wenjuan, Wu Da, Zheng Saijing
Принадлежит: SHANGHAI TOBACCO GROUP CO., LTD.

The present invention provides a gas chromatography—inverse gas chromatography combined analysis device, which includes a gas chromatography column and an inverse gas chromatography column, an input end of the gas chromatography column is connected to a sample feeder, an output end of the gas chromatography column is connected to an input end of the inverse gas chromatography column, the output end of the gas chromatography column is further connected to a first detector, the input end of the inverse gas chromatography column is further connected to a carrier gas tube, an output end of the inverse gas chromatography column is connected to a second detector, and the first detector and the second detector are both connected to a signal collector. The present invention not only can investigate adsorption performance of a tested solid adsorption material with respect to a single probe, but also can investigate adsorption performance of different solid adsorption materials with respect to different constituents in a combined probe at the same time, thereby improving the development efficiency of the inverse gas chromatography technologies. 1. A gas chromatography—inverse gas chromatography combined analysis device , comprising a gas chromatography column and an inverse gas chromatography column , wherein an input end of the gas chromatography column is connected to a sample feeder , an output end of the gas chromatography column is connected to an input end of the inverse gas chromatography column , the output end of the gas chromatography column is further connected to a first detector , the input end of the inverse gas chromatography column is further connected to a carrier gas tube , an output end of the inverse gas chromatography column is connected to a second detector , and the first detector and the second detector are both connected to a signal collector.2. The gas chromatography—inverse gas chromatography combined analysis device as in claim 1 , wherein the ...

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Номер записи: 37
01-08-2013 дата публикации

Similarity evaluating method, similarity evaluating program, and similarity evaluating device for collective data

Номер: US20130197813A1
Автор: Keiichi Noda, Yoshikazu Mori
Принадлежит: Tsumura and Co

Provided is a similarity evaluating device for collective data to evaluate similarity between collective data sets in which a plurality of pieces of data are collected. The device includes a patterning part patterning each data of collective data with a selected scale, a matching number extraction part comparing each patterned data in a round-robin to find numbers of matches, and a matching degree determination part finding a degree of matching on the basis of the found numbers of matches with the use of Tanimoto coefficient, thereby evaluating similarity of the collective data simply and quickly.

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Номер записи: 38
08-08-2013 дата публикации

ELEMENTAL ANALYZER

Номер: US20130199268A1
Автор: HIRANO Akihiro, Yamada Takahiro
Принадлежит: HORIBA, LTD.

In order to provide an elemental analyzer that, without providing a buffer tank, can cope with measurements of a low concentration sample to a high concentration sample on the basis of a simple configuration, the elemental analyzer is provided with: an extraction furnace adapted to heat a sample contained in a crucible R, and gasify an element contained in the sample into sample gas; an introduction flow path L adapted to introduce carrier gas into the extraction furnace a lead-out flow path L adapted to, from the extraction furnace, lead out mixed gas in which the sample gas and the carrier gas are mixed; an elemental analysis part that is provided in the lead-out flow path L and analyzes elements contained in the mixed gas; a bypass flow path L that branches from the introduction flow path L and joins the lead-out flow path L and a valve that is provided in the bypass flow path L and can adjust an opening level. 1. An elemental analyzer comprising:an extraction furnace adapted to heat a sample contained in a crucible, and gasify an element contained in the sample into sample gas;an introduction flow path adapted to introduce carrier gas into the extraction furnace;a lead-out flow path adapted to lead out mixed gas from the extraction furnace, the mixed gas is that the sample gas and the carrier gas are mixed in;an elemental analysis part that is provided in the lead-out flow path and analyzes elements contained in the mixed gas;a bypass flow path that branches from the introduction flow path and joins the lead-out flow path; anda valve that is provided in the bypass flow path and whose opening level is adjustable.2. The elemental analyzer according to claim 1 , wherein the opening level of the valve is adjusted such that a concentration of the sample gas in the mixed gas in the elemental analysis part becomes equal to a predetermined concentration.3. The elemental analyzer according to claim 1 , whereinthe opening level of the valve is adjusted such that a first ...

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Номер записи: 39
08-08-2013 дата публикации

ANALYSIS OF MOLECULAR CONTAMINATION IN VACUUM ENVIRONMENTS

Номер: US20130199269A1
Автор: De Bokx Pieter Klaas, Knobel Hugo Hubertus, Soers Ruud Johannes Theodorus
Принадлежит: KONINKLIJKE PHILIPS ELECTRONICS N.V.

A pre-concentration device is provided for a gas analysis system () for collecting molecular contamination in a vacuum environment (). The pre-concentration device () comprises a hollow element () having an entrance opening () for receiving molecules from the vacuum environment () in a collection phase, a gas outlet for transferring collected molecules to a vacuum compatible detector or second preconcentration device in a transfer phase. The device has an inner wall for adsorbing molecules in the collection phase and desorbing molecules in the transfer phase. The device has a filler element () that is movable from a first position outside the hollow element in the collection phase to a second position inside the hollow element in the transfer phase which second position leaves open a transfer channel to the gas outlet along the inner wall. Advantageously, the device enables transferring of the organic or inorganic contaminants collected in the device under vacuum conditions, and requires a minimal amount of ultra pure gas for the transport of the contaminants to a detector or further a concentration device, which lowers the lower limit of detection. 1. Pre-concentration device for a gas analysis system for detecting molecular contamination in a vacuum environment , the pre-concentration device comprising:a hollow element having a gas entrance opening for receiving gas from the vacuum environment in a collection phase, a gas outlet for transferring gas in a transfer phase, and an inner wall for adsorbing gas in the collection phase and desorbing gas in the transfer phase, anda filler element that is movable from a first position outside the hollow element in the collection phase to a second position inside the hollow element in the transfer phase which second position leaves open a transfer channel to the gas outlet along the inner wall wherein the hollow element has an inner space bounded b the inner wall and the ace has a conical shape, and the filler element has a ...

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Номер записи: 40
08-08-2013 дата публикации

HEMOGLOBIN S ANALYSIS METHOD, HEMOGLOBIN A2 ANALYSIS METHOD, AND HEMOGLOBIN A0 ANALYSIS METHOD

Номер: US20130199277A1
Автор: Taira Hiroaki, Takayuki Oka
Принадлежит:

An object of the present invention is to provide a hemoglobin S analysis method, a hemoglobin A2 analysis method, and a hemoglobin A0 analysis method which enable even highly retentive hemoglobin S, hemoglobin A2 and hemoglobin A0 to be separated in sharp, highly symmetrical peaks by means of cation-exchange high-performance liquid chromatography. 1. A method for analyzing hemoglobin S by cation-exchange high-performance liquid chromatography ,the method comprising utilizing an eluent that contains an azide or a cyanide at a concentration of 0.1 to 50 mmol/L and has a pH of 6.80 to 7.50.2. The method for analyzing hemoglobin S by cation—according to claim 1 ,wherein the eluent contains a salt at a concentration of 500 mmol/L or lower.3. The method for analyzing hemoglobin S by cation—according to claim 1 ,wherein the eluent contains a buffering agent at a concentration of 5 to 500 mmol/L.4. A method for analyzing hemoglobin A2 by cation-exchange high-performance liquid chromatography claim 1 , the method comprising utilizing an eluent that contains an azide or a cyanide at a concentration of 0.1 to 50 mmol/L and has a pH of 6.45 to 6.85.5. The method for analyzing hemoglobin A2 according to claim 4 ,wherein the eluent contains a salt at a concentration of 500 mmol/L or lower.6. The method for analyzing hemoglobin A2 according to claim 4 ,wherein the eluent contains a buffering agent at a concentration of 5 to 500 mmol/L.7. A method for analyzing hemoglobin A0 by cation-exchange high-performance liquid chromatography claim 4 , the method comprising utilizing an eluent that contains an azide or a cyanide at a concentration of 0.1 to 50 mmol/L and has a pH of 6.00 to 6.75.8. The method for analyzing hemoglobin A0 according to claim 7 ,wherein the eluent contains a salt at a concentration of 500 mmol/L or lower.9. The method for analyzing hemoglobin A0 according to claim 7 ,wherein the eluent contains a buffering agent at a concentration of 5 to 500 mmol/L.10. The ...

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Номер записи: 41
08-08-2013 дата публикации

Gel particle measurement reagent and measurement method using same

Номер: US20130203177A1
Автор: Masakazu Tsuchiya, Toru Obata
Принадлежит: Individual

Provided is a gel particle measurement reagent effective in quickly measuring a time point of initiation of production of gel particles. A gel particle measurement reagent R is a gel particle measurement reagent to be used to be agitated continuously with a sample S containing a target substance St as a measuring object to turn the target substance St into gel particles, including: a reagent base material 1 that undergoes a gelation reaction with the target substance St; and a biologically inactive particle formation accelerating factor 2 that is added to the reagent base material, has solubility in the sample S and dissolves therein at a concentration of 0.002 to 1%, and accelerates production of gel particles G whose particle sizes are centered in a predetermined range.

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Номер записи: 42
15-08-2013 дата публикации

GLATIRAMER ACETATE MOLECULAR WEIGHT MARKERS

Номер: US20130205877A1
Автор: Bathoju Ravindra Chary, Devi Basanthi, Ganji Bala Harsha Vardhan, Kallam Sudheer Reddy, Komaravolu Yagna Kiran Kumar, Nallam Chakravarthula Kalyan Narasimham, Ramasamy Karthik, Srinivasan Santhanakrishnan
Принадлежит:

Aspects of the present application relate to molecular weight markers of glatiramer acetate for accurate determination of the average molecular weight of glatiramer acetate. 1. A molecular weight marker having an amino acid composition corresponding to glatiramer acetate and an identified molecular weight which is between about 2 ,000 Daltons and about 40 ,000 Daltons.2. The molecular weight marker of claim 1 , wherein the identified molecular weight is between about 2 claim 1 ,000 Daltons and about 20 claim 1 ,000 Daltons.3. The molecular weight marker of claim 1 , wherein the identified molecular weight is between about 2 claim 1 ,000 Daltons and about 15 claim 1 ,000 Daltons.4. The molecular weight marker of claim 1 , wherein the identified molecular weight is between about 2 claim 1 ,000 Daltons and about 10 claim 1 ,000 Daltons.5. The molecular weight marker of claim 1 , consisting essentially of the amino acids alanine claim 1 , glutamic acid claim 1 , tyrosine claim 1 , and lysine in molar fractions of from about 0.38 to about 0.50 alanine claim 1 , from about 0.13 to about 0.15 glutamic acid claim 1 , from about 0.08 to about 0.10 tyrosine claim 1 , and from about 0.3 to about 0.4 lysine.6. The molecular weight marker of claim 1 , which is molecular weight marker 1 with a peak average molecular weight of 16833 Daltons claim 1 , molecular weight marker 2 with a peak average molecular weight of 8908 Daltons claim 1 , molecular weight marker 3 with a peak average molecular weight of 5006 Daltons claim 1 , or molecular weight marker 4 with a peak average molecular weight of 3709 Daltons.7. A processes for preparing a molecular weight marker of claim 1 , comprising:(a) fractionating the glatiramer acetate polypeptide in a gel permeation chromatography (GPC) column;(b) passing the fractions collected in step (a) through molecular weight cut-off membranes; and(c) isolating the molecular weight markers by lyophilizing the fractions collected from step (b).8. The ...

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Номер записи: 43
15-08-2013 дата публикации

SORAFENIB DIMETHYL SULPHOXIDE SOLVATE

Номер: US20130210865A1
Автор: Jaryal Jagdev Singh, Prasad Mohan, Sathyanarayana Swargam, Sinch Ajay Shankar, Thaper Rajesh Kumar, Zade Sachin Deorao
Принадлежит: RANBAXY LABORATORIES LIMITED

The present invention provides dimethyl sulphoxide solvate of 4-(4-{3-[4-chloro-3-(trifluoromethyl)phenyl]ureido}phenoxy)-N-methylpyridine-2-carboxamide, process for its preparation, pharmaceutical composition comprising it and its use for the treatment of cancer. The present invention also provides a novel HPLC method for the identification, quantification and isolation of related substances of sorafenib. 2. Sorafenib dimethyl sulphoxide solvate according to further characterized by X-ray diffraction peaks at d-spacing 5.36 claim 1 , 4.47 claim 1 , 4.44 claim 1 , 3.26 and 3.14 Å.3. Sorafenib dimethyl sulphoxide solvate of Formula III characterized by X-ray diffraction pattern as depicted in .4. Sorafenib dimethyl sulphoxide solvate of Formula III characterized by DSC thermogram having endotherms at about 123.69° C. and about 202.54° C.5. Sorafenib dimethyl sulphoxide solvate of Formula III characterized by DSC thermogram as depicted in .6. Sorafenib dimethyl sulphoxide solvate of Formula III characterized by X-ray diffraction pattern as depicted in and DSC thermogram as depicted in .7. Sorafenib dimethyl sulphoxide solvate of Formula III characterized by TGA as depicted in .8. Sorafenib dimethyl sulphoxide solvate of Formula III characterized by IR spectrum as depicted in .9. Sorafenib dimethyl sulphoxide solvate of Formula III having purity greater than 99% by HPLC.11. The process according to claim 10 , wherein Sorafenib free base of Formula I is contacted with dimethyl sulphoxide at a temperature of about 15° C. to the reflux temperature of dimethyl sulphoxide.13. The process according to claim 12 , wherein the solvent is selected from the group consisting of water claim 12 , chlorinated hydrocarbons claim 12 , alcohols claim 12 , ketones claim 12 , alkyl acetates claim 12 , ethers and mixtures thereof.14. The process according to claim 12 , wherein sorafenib dimethyl sulphoxide solvate of Formula III is contacted with a solvent at a temperature of about −5° C. ...

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Номер записи: 44
29-08-2013 дата публикации

GAS CHROMATOGRAPHY RECOMPOSITION-OLFACTOMETRY FOR CHARACTERIZATION OF AROMA MIXTURES

Номер: US20130219991A1
Автор: Ebeler Susan E., Hirson Greg, Johnson Arielle
Принадлежит: The Regents of the University of California

A method for the in-instrument recombination of volatiles using a gas chromatograph with mass spectrometry and olfactometry detection is described. Compounds that are introduced into the modified GC are separated conventionally on an analytical capillary GC column. The elution profile of volatiles can be segmented, analyzed and arbitrarily combined. In-line with the GC column, a pneumatic flow switch and splitter are connected to a detector and olfactometer. A cold trap allows the user to build a mixture of separated volatiles that is held until the cryotrap is rapidly heated, releasing the mixture for a subject to smell at the olfactory port and to evaluate. The instrument allows for characterization of the aroma quality of specific fractions of aroma volatiles obtained from foods, flowers or beverages without the need for pure chemical standards or the calculation of individual compound concentrations or sensory thresholds. 1. A method for characterization and analysis of aroma mixtures , comprising:(a) extracting volatiles from a sample to form a mixture of volatiles;(b) separating components of said mixture of volatiles with a gas chromatographic column;(c) detecting eluents from said gas chromatographic column with a detector;(d) dividing eluents from said gas chromatographic column into fractions;(e) combining fractions of eluents from said gas chromatographic column;(f) selecting fractions and combinations of fractions for characterization; and(g) characterizing selected fractions and combined fractions with olfactometry.2. A method as recited in claim 1 , further comprising:creating an elution profile of detected eluents;segmenting the profile to designate eluent fractions; andusing said profile as a map to designate combinations of eluent fractions.3. A method as recited in claim 1 , wherein said eluent from said gas chromatographic column is divided equally over time to designate the fractions.4. A method as recited in claim 1 , further comprising: ...

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Номер записи: 45
29-08-2013 дата публикации

GAS CHROMATOGRAPHY DEVICE

Номер: US20130219992A1
Автор: OKADA MASAYUKI, TERAI YASUNORI
Принадлежит: SHIMADZU CORPORATION

A gas chromatography device adapted for detecting leakage of hydrogen gas without using an air pump and long piping is provided. The gas chromatography device includes: a column oven having a housing, a column disposed in the housing, and a heater heating air in the housing; a sample vaporization chamber supplying a sample to an inlet end of the column; a carrier gas supply section supplying a carrier gas to the sample vaporization chamber; a detector connected to an outlet end of the column; a controller controlling the heater and the carrier gas supply section; and a hydrogen gas detecting sensor. The carrier gas is hydrogen gas. A through hole is formed in the housing and a material that allows hydrogen to pass through but does not transmit heat is disposed in the through hole. The hydrogen gas detecting sensor is disposed near the through hole outside the housing. 1. A gas chromatography device , comprising:a column oven, comprising a housing, a column disposed in the housing, and a heater heating air in the housing;a sample vaporization chamber supplying a sample to an inlet end of the column;a carrier gas supply section supplying a carrier gas to the sample vaporization chamber;a detector connected to an outlet end of the column;a controller controlling the heater and the carrier gas supply section; anda hydrogen gas detecting sensor detecting hydrogen gas,wherein the carrier gas is the hydrogen gas,wherein a through hole is formed in the housing and a material that allows hydrogen to pass through but does not transmit heat is disposed in the through hole, andwherein the hydrogen gas detecting sensor is disposed near the through hole outside the housing.2. The gas chromatography device according to claim 1 , wherein the material is disposed from a center of the through hole to an exterior of the through hole.3. The gas chromatography device according to claim 1 , wherein the hydrogen gas detecting sensor is disposed with a connection tube located between the ...

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Номер записи: 46
29-08-2013 дата публикации

Electronic control of ph and ionic strength

Номер: US20130220830A1
Автор: Annett HAHN-WINDGASSEN, Anton Posch, Aran Paulus, Camille Diges, Elad Brod, Roumen Bogoev, Sricharan BANDHAKAVI, Uri Sivan
Принадлежит: Bio Rad Laboratories Inc, Technion Research and Development Foundation Ltd

Apparatuses and methods for controlling ionic strength and/or pH of a solution are provided.

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Номер записи: 47
29-08-2013 дата публикации

CHROMATOGRAPHIC MEASUREMENT APPARATUS

Номер: US20130224074A1
Автор: NISHIO Tomonori
Принадлежит: FUJIFILM Corporation

Higher economic efficiency is ensured in a chromatographic measurement even when a chromatographic measurement apparatus is used at a place with risk of contamination of testing equipment. A chromatographic measurement apparatus includes an apparatus main body and a measurement unit, wherein the apparatus main body and the measurement unit are configured to be capable of wireless communication of a signal representing measurement information obtained by the measurement unit and capable of mutual pairing setting. The apparatus main body includes a storage section for storing the measurement information, an extracting section for extracting, from a signal obtained via wireless communication, a signal from the measurement unit paired with the apparatus main body based on the pairing setting, and a display section for displaying the measurement information. 1. A chromatographic measurement apparatus for measuring a test article contained in a sample solution , the apparatus comprising:an apparatus main body; andat least one measurement unit for measuring the test article by using an insoluble carrier including a detection area capable of specifically immobilizing the test article,wherein the apparatus main body and each of the at least one measurement unit are configured to be capable of wireless communication of a signal representing measurement information obtained by the measurement unit and capable of mutual pairing setting, andthe apparatus main body comprises a storage section for storing the measurement information, an extracting section for extracting, from a signal obtained via wireless communication, a signal from the measurement unit paired with the apparatus main body based on the pairing setting, and a display section for displaying the measurement information.2. The chromatographic measurement apparatus as claimed in claim 1 , wherein the pairing setting is achieved via non-contact proximity connection.3. The chromatographic measurement apparatus as ...

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Номер записи: 48
19-09-2013 дата публикации

METHOD AND APPARATUS FOR CONTROL OF MASS COMPOSITION OF MOBILE PHASE

Номер: US20130240044A1
Автор: Kirby Peter, Shreve Joshua A.
Принадлежит: WATERS TECHNOLOGY CORPORATION

Described are a method and an apparatus for delivering a fluid having a desired mass composition. According to the method, temperatures of the fluids to be mixed are sensed and the densities of the fluids at the sensed temperatures are determined. The volume of each fluid is determined so that a mixture of the fluids at the sensed temperatures has the desired mass composition. The determined volumes of the fluids are combined to create the mixture. In one option, combining the determined volumes includes metering flows of the fluids sequentially into a common fluid channel. Alternatively, combining the determined volumes includes controlling a flow rate of each of the fluids and directing the fluids into a common fluid channel. 1. A method for delivering a fluid having a desired mass composition , the method comprising: sensing a temperature of the fluid; and', 'determining a density of the fluid at the sensed temperature;, 'for each fluid in a plurality of fluids to be mixed to have a desired mass composition at a reference temperaturedetermining a volume of each of the fluids so that a mixture of the fluids at the sensed temperatures has the desired mass composition; andcombining the determined volumes of the fluids.2. The method of wherein a volumetric composition of the mixture of the fluids at the sensed temperatures is equal to a volumetric composition of a mixture of the fluids at the reference temperature.3. The method of wherein combining the determined volumes of the fluids comprises metering flows of the fluids sequentially into a common fluid channel.4. The method of wherein sensing the temperatures of the fluids comprises sensing a single temperature proximate to a location where the determined volumes are metered.5. The method of wherein combining the determined volumes of the fluids comprises controlling a flow rate of each of the fluids and directing the fluids into a common fluid channel.6. The method of wherein sensing the temperatures of the ...

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Номер записи: 49
26-09-2013 дата публикации

GAS CHROMATOGRAPH

Номер: US20130247650A1
Автор: OKADA MASAYUKI, TERAI YASUNORI
Принадлежит: SHIMADZU CORPORATION

A gas chromatograph for preventing leakage of carrier gas and secondary accident such as explosion is provided. A flow rate restricting valve having valve mechanism which mechanically restricts flow rate of carrier gas is disposed at the upstream side of sample inlet portion of the carrier gas flow path and apart from the flow rate control valve which controls the flow rate of the carrier gas, which is inlet into sample inlet portion and analytical column according to signal from control unit . The excessive flow rate that exceeds the constant flow rate resulted from the leakage of carrier gas is mechanically restricted to a pre-set flow rate by using the flow rate restricting valve. The leakage of carrier gas e.g., helium and the secondary accident due to leakage of carrier gas e.g., hydrogen, are prevented. 1. A gas chromatograph , wherein when a carrier gas filled a gas bomb is inlet into a sample inlet portion and an analytical column , the gas chromatograph is adapted to control a flow rate by using a flow rate control valve disposed in a carrier gas flow path between the gas bomb and the sample inlet portion , the gas chromatograph comprising:a flow rate restricting valve having a valve mechanism and adapted to mechanically restrict the flow rate which is an excessive flow rate that exceeds a constant flow rate of the carrier gas,wherein the flow rate restricting valve is apart from the flow rate control valve and disposed at an upstream side of the sample inlet portion of the carrier gas flow path.2. The gas chromatograph according to claim 1 , wherein the flow rate restricting valve is adapted to restrict the flow rate of the carrier gas to a pre-set flow rate which is less than the constant flow rate which is the excessive flow rate that exceeds the constant flow rate of the carrier gas.3. The gas chromatograph according to claim 1 , wherein the flow rate restricting valve is adapted to close the carrier gas flow path and cut off the carrier gas flow rate ...

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Номер записи: 50
26-09-2013 дата публикации

METHOD FOR EVALUATING SENSORY STIMULUS COMPONENT

Номер: US20130247654A1
Автор: SAIMA Toshinori, SUGIMOTO Daisuke, Yaguchi Yoshihiro, ZAIZEN Kyoko
Принадлежит: TAKASAGO INTERNATIONAL CORPORATION

A method for evaluating a sensory stimulus component in a test component with a taste-analyzing liquid chromatograph system. The method includes: measuring the test component with the taste-analyzing liquid chromatograph system to obtain taste evaluation data; and evaluating a persistence of a sensory stimulus intensity of the test component from a continuous intensity change of the test component using the taste evaluation data as an index. 1. A method for evaluating a sensory stimulus component in a test component with a taste-analyzing liquid chromatograph system ,the method comprising:measuring the test component with the taste-analyzing liquid chromatograph system to obtain taste evaluation data; andevaluating a persistence of a sensory stimulus intensity of the test component from a continuous intensity change of the test component using the taste evaluation data as an index.2. The method according to claim 1 , wherein a plurality of similar test components is measured with the taste-analyzing liquid chromatograph system to obtain taste evaluation data claim 1 , and the test components are distinguished into similar groups to evaluate the persistence of sensory stimulus intensities of the test components using the taste evaluation data as an index.3. The method according to claim 1 , wherein the taste-analyzing liquid chromatograph system includes:(a) a taste detecting part for detecting the sensory stimulus component in the test component over a time course, which includes a plurality of taste sensors each of which has a different response characteristic; and(b) a signal processing part that processes a detection signal obtained from the plurality of the taste sensors to determine taste evaluation data concerning each sensory stimulus component.4. The method according to claim 3 , wherein the taste evaluation data is measured by using an intensity of a sensor reaction of the taste sensor as an index.5. The method according to claim 4 , wherein the evaluation ...

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Номер записи: 51
26-09-2013 дата публикации

HPLC CAPILLARY COLUMN DEVICE

Номер: US20130248700A1
Автор: Benevides Christopher C., Collamati Robert, Compton Bruce J., Dourdeville Theodore
Принадлежит:

A high pressure liquid chromatography (HPLC) capillary column device, system and method for processing a HPLC sample with a cartridge housing a packed capillary column; at least one inlet connection to the capillary column for a sample fluid; and at least one outlet connection from the capillary column for the sample fluid. The outlet connection is able to accommodate either a spray tip for atomizing the sample fluid or a transport tube for transporting the sample fluid from a spray tip column to a spray tip. Inlet connections enable supply of electrical power to the capillary column through electrical connections disposed within the cartridge housing; and gas for evaporating the sample liquid is supplied to at least one outlet connection from the capillary column for the sample fluid through a gas supply line within the cartridge housing. The temperature of the sample liquid can be controlled through a heat connection. 1. A liquid chromatography column device for processing a sample , the device having a cartridge housing , the cartridge housing comprising:a column inside the cartridge housing; andat least three connections in communication with the column, the at least three connections comprising a first inlet connection that includes an electrical connection and a second inlet connection that receives a sample fluid; andat least one outlet connection in communication with the column.2. The device of claim 1 , wherein the column is a packed column.3. The device of claim 1 , further comprising a plurality of electrical connections positioned inside the cartridge housing claim 1 , and wherein the electrical connection provides electrical power to the column through the electrical connections positioned inside the cartridge housing.4. The device of claim 1 , wherein the at least three connections comprises a third inlet connection that receives a gas.5. The device of claim 4 , wherein the third inlet connection supplies the gas to the at least one outlet connection ...

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Номер записи: 52
03-10-2013 дата публикации

Continuous processing methods for biological products

Номер: US20130260419A1
Автор: Marc A. T. Bisschops, Thomas C. Ransohoff
Принадлежит: TARPON BIOSYSTEMS Inc

The present invention is directed to the development of continuous processing technology for the purification of biopharmaceuticals and biological products, such as monoclonal antibodies, protein therapeutics, and vaccines. Methods for continuous processing of a biological product in a feed stream toward formulation of a purified bulk product are described.

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Номер записи: 53
17-10-2013 дата публикации

Chromatographic Optical Detection System

Номер: US20130269424A1
Автор: Jarrell Joseph A.
Принадлежит: WATERS TECHNOLOGIES CORPORATION

A chromatographic optical detection system includes an optical detector disposed to receive light scattered from a stream of particles and configured to convert the received light to an electrical signal; a signal-processing unit in signal communication with the optical detector to receive the electrical signal, and configured to convert the electrical signal to digital pulses and count the digital pulses to output a first signal corresponding to a number of particles detected in a time interval, and configured to integrate and digitize the electrical signal to output a second signal corresponding to the number of particles detected in the time interval; and a data station in signal communication with the signal-processing unit, and configured to select the first signal, if the number of particles detected in the time interval is less than a threshold criterion, and to select the second signal if the number of particles detected in the time interval exceeds the threshold criterion. The threshold criterion is associated with a saturation condition. 1. A chromatographic optical detection system comprising:an optical detector disposed to receive light scattered from a stream of particles and configured to convert the received light to an electrical signal;a signal-processing unit in signal communication with the optical detector to receive the electrical signal, and configured to convert the electrical signal to digital pulses and count the digital pulses to output a first signal corresponding to a number of particles detected in a time interval, and to integrate and digitize the electrical signal to output a second signal corresponding to the number of particles detected in the time interval; anda data station in signal communication with the signal-processing unit, and configured to select the first signal, if the number of particles detected in the time interval is less than a threshold criterion, and to select the second signal, if the number of particles detected ...

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Номер записи: 54
17-10-2013 дата публикации

Stable Solid Formulation of GC-C Receptor Agonist Polypeptide Suitable for Oral Administration

Номер: US20130273169A1
Автор: Alfredo Grossi, Angelika Fretzen, Hong Zhao, Mahendra Dedhiya, Steven Witowski, Yun Mo
Принадлежит: Ironwood Pharmaceuticals Inc

Solid, stable formulations of linaclotide suitable for oral administration are described herein as are methods for preparing such formulations. The formulations described herein contain a polypeptide consisting of the amino acid sequence Cys Cys Glu Tyr Cys Cys Asn Pro Ala Cys Thr Gly Cys Tyr (“linaclotide”; SEQ ID NO:1) or a pharmaceutically acceptable salt thereof. The linaclotide formulations described herein are stable and have a sufficient shelf life for manufacturing, storing and distributing the drug.

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Номер записи: 55
24-10-2013 дата публикации

Portable Gas Analyzer

Номер: US20130276512A1
Автор: Bae Byunghoon, Kang Taekyu, KIM Jihyung, Shannon Mark A.
Принадлежит: The Board of Trustees of the University of Illinois

A portable gas analyzer comprising an integrated micro-flame ionization detector (micro-FID), a micro-gas chromatograph (micro-GC), an electrolyzer, and a flame-shaped electrode are provided. The components of the portable gas analyzer can be integrated into a single “lunchbox” sized housing with all the peripherals required to operate the micro-GC/FID without an external power and gas supply. 1. A portable gas analyzer system comprising:a micro gas chromatograph;a micro flame ionization detector;a flame-shaped electrode in the micro flame ionization detector; anda water electrolyzer.2. The system of claim 1 , wherein the electolyzer provides substantially all of the oxygen and hydrogen for the flame ionization detector.3. The system of claim 1 , wherein the electrolyzer contains a plurality of gas outlet ports each connected to a desiccant tube.4. The system of claim 3 , wherein the desiccant tube reduces the humidity of gas from the gas outlet ports to less than 5%.5. The system of claim 1 , wherein the micro gas chromatograph comprises a microcolumn having a silicon substrate.6. The system of claim 1 , wherein the micro gas chromatograph comprises a preconcentrator.7. The system of claim 5 , wherein the microcolumn comprises a fusion bonded silicon substrate.8. The system of claim 7 , wherein the fusion bonded silicon substrate comprises two fused silicon plates.9. The system of claim 8 , wherein each of the two silicon plates contain a microchannel.10. The system of claim 1 , wherein the micro flame ionization detector comprises a silicon layer.11. The system of claim 10 , wherein the silicon layer is from about 400 to 1000 μm thick.12. The system of claim 11 , wherein the silicon layer is about 750 μm thick.13. The system of claim 10 , wherein the silicon layer is situated between two quartz plates.14. The system of claim 13 , wherein at least one of the quartz plates contains an exhaust hole.15. The system of claim 10 , wherein the silicon layer comprises an ...

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Номер записи: 56
24-10-2013 дата публикации

METHODS FOR DIAGNOSING AND ASSESSING KIDNEY DISEASE

Номер: US20130276513A1
Автор: NAVIAUX Robert, SHARMA Kumar
Принадлежит:

The technology relates in part to methods for identifying the presence of kidney disease, determining the level of kidney disease, or the progression of kidney disease, in a subject that has or has not been diagnosed with diabetes. The technology further relates to methods for determining the targets for therapy for kidney disease, the efficacy of a treatment for kidney disease, and methods for determining the toxicity of a therapeutic in a subject with kidney disease. The technology relates in part to methods for identifying the presence of kidney disease, determining the level of kidney disease, or the progression of kidney disease, in a subject that has or has not been diagnosed with diabetes. The technology further relates to methods for determining the targets for therapy for kidney disease, the efficacy of a treatment for kidney disease, and methods for determining the toxicity of a therapeutic in a subject with kidney disease. 1. A method of identifying the presence or level of kidney disease in a subject , comprisinga. determining the level of at least one organic acid selected from the group consisting of glycolic acid, 3-OH isobutyric acid, 3-OH isovaleric acid, aconitic acid, homovanillic acid, citric acid, uracil, fumaric acid, oleic acid and azelaic acid in a sample obtained from the subject; i. the reference level has been determined from at least one sample collected from the same subject at a different time period; or', 'ii. the reference level has been determined from a sample or samples collected from one or more other subjects; and, 'b. comparing the level of the at least one organic acid with a reference level of the at least one organic acid, wherein'}c. identifying the presence or level of kidney disease in the subject where the at least one organic acid level in the subject is decreased when compared to the reference level of the at least one organic acid.2. The method of claim 1 , wherein the level of:(a) at least two organic acids selected ...

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Номер записи: 57
24-10-2013 дата публикации

Rotary Shear Injector Valve With Displaced Rotor Grooves

Номер: US20130276520A1
Автор: Moeller Mark W.
Принадлежит: WATERS TECHNOLOGIES CORPORATION

A valve includes a stator that has fluidic ports in a seal surface, and a rotor that has a seal surface contacting the stator's seal surface. The rotor has through-holes that extend from the seal surface to a back surface, providing fluidic communication with a conduit disposed adjacent to the back surface of the rotor. A chromatography apparatus includes a sample source, a solvent source having a higher operating pressure than the sample source, and an injector valve. A method of performing chromatography includes loading a sample with a valve in a load-state configuration that disposes a sample source in fluidic communication with a conduit disposed adjacent to a back surface of the rotor, and injecting the loaded sample with the valve in an inject-state configuration that disposes a solvent source in fluidic communication with a column via at grooves adjacent to a front seal surface of the rotor. 1. A rotary shear chromatography valve , comprising:a stator defining at least one fluidic port in a seal surface of the stator; anda rotor, having a seal surface contacting the seal surface of the stator, and defining at least one through-hole that extends from the seal surface of the rotor to a back surface of the rotor and provided fluidic communication with a conduit disposed adjacent to the back surface of the rotor.2. The valve of claim 1 , wherein the conduit comprises a groove defined in the back surface of the rotor.3. The valve of claim 1 , wherein the rotor defines at least six through-holes that provide fluidic communication with at least three conduits disposed adjacent to the back surface of the rotor.4. The valve of claim 1 , further comprising a back plate having a seal surface in contact with the back surface of the rotor.5. The valve of claim 4 , wherein the conduit comprises a groove in the seal surface of the back plate.6. The valve of claim 4 , wherein the back plate has a contact area with the rotor that is greater than a contact area between the ...

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Номер записи: 58
24-10-2013 дата публикации

ApoIII and the Treatment and Diagnosis of Diabetes

Номер: US20130280816A1
Автор: Berggren Per-Olof
Принадлежит:

The present invention provides methods of identifying candidate compounds for the treatment of type I diabetes comprising contacting pancreatic β cells with an amount of apolipoprotein CIII (“apoCIII”) effective to increase intracellular calcium concentration, in the presence of one or more test compounds, and identifying those test compounds that inhibit an apoCIII-induced increase in intracellular calcium concentration in the pancreatic β cells. The present invention also provides methods for treating patients with type I diabetes comprising administering to the patient an amount effective of an inhibitor of apoCIII to reduce apoCIII-induced increase in intracellular calcium concentration in pancreatic β cells. 15-. (canceled)6. (canceled)7. A method for diagnosing a propensity to develop Type 1 diabetes in a subject , comprising:(a) measuring an amount of sialylated apoCIII in a blood serum sample from the subject;(b) comparing the amount of sialylated apoCIII measured in step (a) with an amount of sialylated apoCIII in a control blood serum sample; and(c) diagnosing those subjects with an elevated amount of sialylated apoCIII measured in step (a) relative to the amount of sialylated apoCIII in the control blood serum sample as having Type 1 diabetes.8. The method of claim 7 , wherein the sialylated apoCIII is mono-sialylated apoCIII.9. The method of claim 7 , wherein the sialylated apoCIII is di-sialylated apoCIII.10. The method of claim 7 , wherein the sialylated apoCIII is both mono- and di-sialylated apoCIII. This application a Continuation of U.S. application Ser. No. 12/196,536, Filed: Aug. 22, 2008, which is a Divisional of U.S. application Ser. No. 10/834,525, Filed: Apr. 29, 2004, which claims priority to U.S. Provisional Patent Application 60/466,517 filed Apr. 29, 2003.Voltage-gated L-type Ca-channels have an important physiological role in pancreatic β-cell (“β-cell”) signal-transduction (1). These channels constitute an essential link between ...

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Номер записи: 59
14-11-2013 дата публикации

FITTING ELEMENT WITH BIO-COMPATIBLE SEALING

Номер: US20130298647A1
Автор: Falk-Jordan Stefan
Принадлежит: AGILENT TECHNOLOGIES, INC.

A fitting element is configured for coupling tubing to a fluidic device having a receiving cavity configured for receiving the fitting element, where the tubing has an inner contact surface of a biocompatible material, the inner contact surface being configured to contact a fluid to be conducted by the tubing, and the receiving cavity having a receiving contact surface of a bio-compatible material. The fitting element includes a first sealing element of a bio-compatible material configured for sealing to the bio-compatible material of the inner contact surface of the tubing, and a second sealing element configured for sealing against a pressure ambient to a pressure of the fluid in the tubing. Upon coupling of the tubing to the fluidic device, at least a portion of the receiving contact surface, the first sealing element, and the second sealing element enclose an interspace, each surface of the interspace being a bio-compatible material. 1. A fitting element for coupling a tubing to a fluidic device having a receiving cavity configured for receiving the fitting element , wherein the tubing has an inner contact surface comprising a biocompatible material , the inner contact surface being configured to contact a fluid to be conducted by the tubing , and the receiving cavity having a receiving contact surface comprising a bio-compatible material , the fitting element comprising:a first sealing element comprising a bio-compatible material and being configured for sealing to the bio-compatible material of the inner contact surface of the tubing, anda second sealing element configured for sealing against a pressure ambient to a pressure of the fluid in the tubing,wherein, upon coupling of the tubing to the fluidic device, at least a portion of the receiving contact surface, the first sealing element, and the second sealing element enclose an interspace, each surface of the interspace comprising a bio-compatible material.2. The fitting element of claim 1 , wherein the ...

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Номер записи: 60
14-11-2013 дата публикации

AUTOMATICALLY CONTROLLING A PLURALITY OF DEVICES OF A SEPARATION AND DETECTION PROCESS FOR QUANTITATIVE SAMPLE ANALYSIS

Номер: US20130303409A1
Автор: Kapps Martin
Принадлежит:

A control system () for automatically controlling a plurality of devices () of a separation and detection process for quantitative sample analysis and according computer program. Such systems and computer programs can be used to operate quantitative sample analysis devices such as for example high performance liquid chromatography (HPLC) devices or the like. 1121112131416. A control system () for automatically controlling a plurality of devices () of a separation and detection process for quantitative sample analysis , comprising a data storage () , a device modelling unit () , a sample modelling unit () , a sequence generating unit () and an interface unit () , wherein{'b': 11', '2', '12', '2', '2', '11, 'the data storage () is arranged to hold characteristic data of each of the devices () and the device modelling unit () is arranged to model the devices () using the characteristic data of the devices () held in the data storage ();'}{'b': 11', '3', '13', '3', '11, 'the data storage () is arranged to hold data of source samples () and the sample modelling unit () is arranged to generate a plurality of analytical samples to be analysed in the separation and detection process for quantitative sample analysis using the data of source samples () held in the data storage ();'}{'b': 14', '2, 'the sequence generating unit () is arranged to define an analytical sample sequence of the analytical samples within the separation and detection process for quantitative sample analysis taking into account utilization of the devices (); and'}{'b': 16', '2', '14, 'the interface unit () is arranged to operate the devices () of the separation and detection process for quantitative sample analysis in accordance with the analytical sample sequence defined by the sequence generating unit ().'}21151115141116215. The control system () according to claim 1 , comprising a method modelling unit () wherein the data storage () is arranged to hold data of methods for the separation and detection ...

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Номер записи: 61
21-11-2013 дата публикации

LIQUID COMPOSITIONS FOR MARKING LIQUID HYDROCARBON MOTOR FUELS AND OTHER FUELS, MOTOR FUELS AND OTHER FUELS CONTAINING THEM AND PROCESS FOR DETECTING THE MARKERS

Номер: US20130305596A1
Автор: AMBLARD Bénédicte, Fadel Denis, Mercier Jean-Paul, TORT Frédéric, Tremoliere Christian
Принадлежит: TOTAL MARKETING SERVICES

The present disclosure includes liquid compositions that can be used for marking liquid hydrocarbon-based fuels and combustibles; these compositions include at least one marker, one or more solvents and, optionally, one or more functional additives other than the markers. The disclosure also includes a process for qualitative and quantitative detection of these markers present in a liquid hydrocarbon-based composition. 1. A liquid composition comprising: tricyclodecenyl isobutyrate (3a,4,5,6,7,7a-hexahydro-4,7-methano-1h-inden-5 (or 6)-yl) (CAS 67634-20-2);', 'tricyclodecenyl propionate (CAS 17511-60-3);', 'cis-3-hexenyl acetate (CAS 3681-71-8);', 'ethyl linalool (CAS 10339-55-6);', 'prenyl acetate (CAS 1191-16-8);', 'ethyl myristate (CAS 124-06-1);', 'para-tert-butylcyclohexyl acetate (CAS 32210-23-4);', 'butyl acetate (CAS 123-86-4);', 'tricyclodecenyl acetate (4,7-methano-1h-inden-6-ol, 3a,4,5,6,7,7a-hexahydro-) (CAS 5413-60-5);', 'ethyl caprate(CAS 110-38-3); and, '(a) at least one marker, chosen from the following aliphatic or cycloaliphatic compounds;'}(b) [[b)]] a solvent or a mixture of solvents.2. The liquid composition according to for use to mark motor fuels and other liquid hydrocarbon fuels in which it is incorporated.3. Motor fuels or liquid hydrocarbon fuels comprising: tricyclodecenyl isobutyrate (3a,4,5,6,7,7a-hexahydro-4,7-methano-1h-inden-5 (or 6)-yl) (CAS 67634-20-2);', 'tricyclodecenyl propionate (CAS 17511-60-3);', 'cis-3-hexenyl acetate (CAS 3681-71-8);', 'ethyl linalool (CAS 10339-55-6);', 'prenyl acetate (CAS 1191-16-8);', 'ethyl myristate (CAS 124-06-1);', 'para-tent-butylcyclohexyl acetate (CAS 32210-23-4);', 'butyl acetate (CAS 123-86-4);', 'tricyclodecenyl acetate (4,7-methano-1h-inden-6-ol, 3a,4,5,6,7,7a-hexahydro-) (CAS 5413-60-5);', 'ethyl caprate(CAS 110-38-3); and, '(a) at least one marker, chosen from the following aliphatic or cycloaliphatic compounds(b) a solvent or a mixture of solvents.4. A process for the qualitative detection ...

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Номер записи: 62
21-11-2013 дата публикации

GELC-MS USING STAIN FREE TECHNOLOGY

Номер: US20130306854A1
Автор: Belisle Christopher
Принадлежит:

Disclosed herein is a method of preparing a protein sample for mass spectroscopy. The method includes separating proteins of the sample on an electrophoresis gel; contacting the proteins with a halo-substituted organic compound; exposing the gel to UV light; detecting fluorescence emitted from the electrophoresis gel; excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; and subjecting proteins from the at least one portion to mass spectroscopy. Using this method, more proteins can be identified by GeLC-MS than when the electrophoresis gel is treated with a protein stain or subjected to the gel handling steps accompanying such treatment. 1. A method of preparing a protein sample for mass spectroscopy , the method comprising:providing an electrophoresis gel comprising the protein sample, wherein proteins of the protein sample have been separated by electrophoresis;contacting the protein sample with a halo-substituted compound;exposing the electrophoresis gel to UV light;detecting fluorescence emitted from the electrophoresis gel;excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; andsubjecting proteins from the at least one portion to mass spectroscopy.2. The method of claim 1 , wherein the halo-substituted compound is a component of the electrophoresis gel and said contacting occurs upon separating proteins of the protein sample by electrophoresis.3. The method of claim 1 , wherein the halo-substituted compound is selected from the group consisting of chloroform claim 1 , trichloroethanol claim 1 , trichloroacetate claim 1 , and 3-bromo-1-propanol.4. The method of claim 1 , wherein the UV light has a wavelength in the range of about 200 nm to about 400 nm.5. The method of claim 1 , wherein the fluorescence emitted from the ...

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Номер записи: 63
28-11-2013 дата публикации

CO2-REMOVAL DEVICE AND METHOD

Номер: US20130312500A1
Автор: Riviello John M.
Принадлежит:

An electrolytic CO-removal device for anion analysis of a liquid sample. The device includes a basic chamber and CO-permeable tubing in the basic chamber. Anion exchange membranes are disposed on opposite sides of the basic chamber, and electrodes are disposed outside the membranes. The device can be integral with a suppressor in an ion chromatography system and/or an aqueous stream purifier. Also, methods performed by the device. 1. A method for removing COfrom an aqueous liquid sample stream containing CO , said method comprising{'sub': 2', '2', '2', '2', '2, '(a) flowing said aqueous liquid sample stream containing COthrough a liquid sample stream flow channel in an electrolytic CO-removal device on one side of a CO-permeable barrier from a basic chamber comprising basic medium comprising an aqueous cation hydroxide solution, said CO-permeable barrier permitting the passage of COgas but substantially blocking the passage of water; and'}(b) passing a current through a first anion exchange membrane on one side of said basic chamber from a cathode on the opposite side of said first anion exchange membrane from said basic chamber, said current passing through said basic chamber and a second anion exchange membrane on the opposite side of said basic chamber from said first anion exchange membrane to an anode on the opposite side of said basic chamber from said second anion exchange membrane, to regenerate said basic medium.2. The method of in which said CO-permeable barrier is substantially non-retentive electrostatically for charged ionic species.3. The method of in which at least 90% of the COin said liquid sample stream is removed while performing said method.4. The method of in which said cation hydroxide solution is non-flowing while performing said method.5. The method of in which said basic medium further comprises anion exchange packing.6. The method of in which said CO-permeable barrier comprises tubing claim 1 , said liquid sample stream flow channel ...

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Номер записи: 64
05-12-2013 дата публикации

BUBBLE REDUCTION DEVICE, CHROMATOGRAPHY DEVICE, BUBBLE REDUCTION METHOD, AND BUBBLE REDUCTION PROGRAM

Номер: US20130319087A1
Автор: Kasai Tokuo, MATSUBARA Takeshi, SATAKE Seiji, Sezaki Akira
Принадлежит: ARKRAY, INC.

A bubble reduction device, chromatography device, bubble reduction method and bubble reduction program capable of reducing bubbles in an eluent. Included are a liquid accommodation portion, a liquid supply apparatus, an air layer formation apparatus, a first channel and an evacuation portion. The liquid accommodation portion accommodates a liquid that is to elute an analysis component from a specimen adsorbed to an adsorption portion. The liquid supply apparatus, by operation of a rod pushing up and polling down, sucks and discharges the liquid through an aperture portion of a tube portion, the aperture portion being oriented upward. The air layer formation apparatus forms an air layer in the tube portion. The first channel connects the liquid supply apparatus with the liquid accommodation portion. The evacuation portion is connected to the first channel via a first switching valve and evacuates the air layer through the first channel. 1. A bubble reduction device comprising:a liquid accommodation portion that accommodates a liquid that is to elute an analysis component from a specimen adsorbed to an adsorption portion;a liquid supply apparatus that, by operation of a rod pushing up or pulling down, sucks or discharges the liquid through an aperture portion of a tube portion, the aperture portion being oriented upward;an air layer formation apparatus that forms an air layer in the tube portion;a first channel that connects the liquid supply apparatus with the liquid accommodation portion; andan evacuation portion that is connected to the first channel via a first switching valve and that evacuates the air layer through the first channel.2. The bubble reduction device according to claim 1 , wherein the air layer formation apparatus includes an atmosphere release valve provided at the first channel claim 1 , andthe air layer is introduced into the tube portion through the first channel by an operation of the rod pushing up or pulling down in a state in which the ...

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Номер записи: 65
05-12-2013 дата публикации

LIQUID CHROMATOGRAPHY APPARATUS, LIQUID CHROMATOGRAPHY ANALYSIS METHOD, AND LIQUID CHROMATOGRAPHY ANALYSIS PROGRAM

Номер: US20130319088A1
Автор: Kasai Tokuo, SATAKE Seiji
Принадлежит: ARKRAY, INC.

A liquid chromatography apparatus having: a column that adsorbs analysis components within a specimen; a plunger pump that feeds eluent A, that elutes the analysis components adsorbed at the column, in an amount greater than or equal to an amount needed for analysis of one specimen from a cylinder portion by a one-time pushing operation of a rod; a photometric unit that analyzes analysis components eluted by eluent A; an eluent loop that holds eluent B; a liquid feeding flow path that communicates the plunger pump and the column; and a first switching valve that switches the liquid feeding flow path to either of a first flow path, that causes eluent A to flow from the plunger pump to the column, and a second flow path, that causes eluent A to flow from the plunger pump through the first eluent holding loop to the column, is provided. 1. A liquid chromatography apparatus comprising:an adsorbing portion that adsorbs analysis components within a specimen;a liquid feeding device that feeds a first eluent, that elutes analysis components adsorbed at the adsorbing portion, in an amount greater than or equal to an amount needed for analysis of one specimen from a cylinder portion by a one-time pushing operation of a rod;a liquid feeding flow path that communicates the liquid feeding device and the adsorbing portion; andan analyzing unit for analyzing analysis components eluted by the first eluent.2. The liquid chromatography apparatus of claim 1 , further comprising:a first holding flow path that holds a second eluent that is different than the first eluent; andfirst switching unit for switching the liquid feeding flow path to either of a first flow path, that causes the first eluent to flow from the liquid feeding device to the adsorbing portion, and a second flow path, that causes the first eluent to flow from the liquid feeding device through the first holding flow path to the adsorbing portion.3. The liquid chromatography apparatus of claim 2 , further comprising:a ...

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Номер записи: 66
05-12-2013 дата публикации

Device and Method for Field Flow Fractionation

Номер: US20130319945A1
Автор: Johann Christoph, Rosch Ulrich
Принадлежит: WYATT TECHNOLOGY EUROPE GMBH

A device for field flux fractioning comprises a sensor to determine the presence of particles in a liquid and a channel impermeable for particles and permeable for liquid. A pump conveys the liquid to first and second paths to the channel, where the second path connects in a first position to the pump outlet and in a second position to the sensor. An injection device injects a sample comprising particles into the liquid flowing through the first path. A distribution device distributes the flow volume conveyed by the pump in a first position at a predetermined ratio to the first and second paths. A control device comprises a valve and a measuring device to measure the flow volume. The valve controls the flow volume in the first path in consideration of the measuring device measurement and the pump conveys the liquid in a flow volume, which can be precisely dosed. 1. A device for field flux fractioning , witha sensor for determining the presence of particles in a carrier liquid,at least one essentially closed oblong channel, which comprises a first end with a first connection and a second end with a second connection and its wall comprises at least one section extending in the longitudinal direction of the channel, impermeable for the particles but permeable for the carrier liquid,a pump, which comprises an inlet and an outlet and is provided to convey the carrier liquid,a first liquid path, which connects the outlet of the pump to a first connection of the channel,a second liquid path, which connects the second connection of the channel to a first switching means, through which the second liquid path can be connected in a first switch position to the outlet of the pump and in a second switch position to the sensor,an injection device, which is arranged in the first liquid path and provided to inject a sample comprising particles into the carrier liquid flowing through the first liquid path, anda flow volume—distribution device, which divides the flow volume conveyed ...

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Номер записи: 67
05-12-2013 дата публикации

Copolymer-1, process for preparation and analytical methods thereof

Номер: US20130323771A1
Автор: Anindya Sibnath Bhattacharyya, Avinash Venkatraman Naidu, Dhananjay Govind Sathe, Divya Lal Saksena, Rakesh Shekhawat, Ramanujam Sukumar, Sanjay Vyankatrao Patil, Sundaram Subramanian
Принадлежит: USV Pvt Ltd

The present invention relates to analytical methods such as molecular weight determination of polypeptide, in particular Glatiramer acetate. The present invention further relates to an improved process for preparation of polypeptides or pharmaceutically acceptable salts thereof, particularly Glatiramer acetate also known as Copolymer-1. The present invention further relates to characterization of Glatiramer acetate by peptide mapping.

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Номер записи: 68
12-12-2013 дата публикации

SPECTROSCOPIC SAMPLE INSPECTION FOR AUTOMATED CHROMATOGRAPHY

Номер: US20130327129A1
Автор: Jung Moon Chul
Принадлежит:

A method and a system for generating an extraction sample from a dried sample spot. The method includes acquiring a spectroscopic image of an analyte in the spot. The spectroscopic image includes information on a spatial distribution of the analyte. A region of the spot to be extracted is determined from the spectroscopic image and an extraction sample is generated from the determined region. In other embodiments, the method and system are based on acquiring a spectroscopic measurement of an analyte in the dried sample spot, determining a quantity of the analyte in the spot based on the spectroscopic measurement and determining a volume of an extraction solvent for creating an extraction sample from the spot. The determined volume is based on the determined quantity of the analyte and a detection characteristic of an analytical measurement system. In other embodiments, the method is applied to solid samples. 1. A method of generating an extraction sample from a dried sample spot , the method comprising:acquiring a spectroscopic image of an analyte in a dried sample spot, the spectroscopic image comprising information on a spatial distribution of the analyte in the dried sample spot;determining a region of the dried sample spot for extraction based upon the spectroscopic image; andgenerating an extraction sample from the determined region of the dried sample spot.2. The method of wherein the spectroscopic image is a Raman spectroscopy image.3. The method of wherein the dried sample spot is disposed on a sample carrier4. The method of wherein the sample carrier is a dried blood spot collection card.5. The method of wherein the determination of a region of the dried sample spot for extraction is based on a predetermined aperture.6. The method of wherein the predetermined aperture is based on a diameter of at least one of an outlet port of a supply conduit and an inlet port of an extraction conduit used to supply an extraction solvent to the dried sample spot and to ...

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Номер записи: 69
19-12-2013 дата публикации

MOISTURE SENSOR INCLUDING, AS A MOISTURE-ABSORBING LAYER, A POLYMER LAYER INCLUDING A MIXTURE OF POLYAMIDES

Номер: US20130336842A1
Автор: Grange Hubert
Принадлежит: Commissariat A L'Energie Atomique Et Aux Energies Alternatives

The invention relates to a humidity sensor including, as a humidity absorbent layer, a polymer layer including a blend including a first polyamide and a second polyamide, where the said second polyamide includes, in its repetitive units, a number of carbon atoms greater than that of the repetitive units of the first polyamide. 1. A humidity sensor including , as a humidity absorbent layer , a polymer layer including a blend including a first polyamide and a second polyamide , where the said second polyamide includes , in its repetitive units , a number of carbon atoms greater than that of the repetitive units of the first polyamide.2. A humidity sensor according to claim 1 , in which the polymer layer consists solely of polyamides.3. A humidity sensor according to claim 1 , wherein the first polyamide is chosen from among polyamide 6 claim 1 , polyamide 6-6 and polyamide 11 claim 1 , and the second polyamide is chosen from among polyamide 6-6 claim 1 , polyamide 6-10 and polyamide 12.4. A humidity sensor according to claim 1 , wherein the polymer layer includes a blend chosen from among the following blends:a blend of polyamide 6 and polyamide 6-6;a blend of polyamide 6 and polyamide 6-10;a blend of polyamide 6-6 and polyamide 6-10;a blend of polyamide 11 and polyamide 12.5. A humidity sensor according to claim 1 , wherein the polymer layer includes a blend of polyamide 6 and polyamide 6-6.6. A humidity sensor according to claim 1 , wherein claim 1 , in the blend claim 1 , the first polyamide is present relative to the second polyamide in a mass proportion of between 95/5 and 5/95.7. A humidity sensor according to claim 1 , including at least one said polymer layer claim 1 , positioned between a first electrode and a second electrode.8. A humidity sensor according to claim 7 , in which the first electrode and the second electrode are in contact with the same substrate.9. A humidity sensor according to claim 1 , including a beam or a membrane covered claim 1 , wholly ...

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Номер записи: 70
26-12-2013 дата публикации

Methods for determining anti-drug antibody isotypes

Номер: US20130344621A1
Автор: Linda Ohrmund, Scott Hauenstein, Sharat Singh, Shui Long Wang
Принадлежит: Nestec SA

The present invention provides assay methods for the determination of one or more anti-drug antibody (ADA) isotypes in a sample. As a non-limiting example, the assays of the present invention are particularly useful for determining different ADA isotypes in samples from ADA-positive patients receiving an anti-TNFα drug such as REMICADE™ (infliximab) or HUMIRA™ (adalimumab). The present invention also provides methods for optimizing therapy and/or reducing toxicity in subjects receiving TNFα inhibitors for the treatment of TNFα-mediated disease or disorders.

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Номер записи: 71
02-01-2014 дата публикации

PHARMACEUTICAL COMPOSITIONS AND METHODS FOR PRODUCING LOW IMPURITY CONCENTRATIONS OF THE SAME

Номер: US20140005233A1
Автор: Ding Min, Flood Keith, Krishna Gopal, Motheram Rajeshwar, Ramakrishna Kornepati
Принадлежит: The Medicines Company

A composition having clevidipine as an active ingredient is described. The composition includes clevidipine as an active ingredient and an amount of the impurity H168/79 that is no greater than about 1.5%, or where the ratio between clevidipine and H168/79 is equal or above 60 to 1. 2. The composition of claim 1 , wherein the effective temperature is approximately equal to or less than about 25° C.3. The composition of claim 1 , wherein the effective temperature is approximately equal to or less than about 5° C.5. The composition of claim 4 , wherein the effective temperature is approximately equal to or less than about 25° C.6. The composition of claim 4 , wherein the effective temperature is approximately equal to or less than about 5° C.8. The composition of claim 7 , wherein the effective temperature is approximately equal to or less than about 25° C.9. The composition of claim 7 , wherein the effective temperature is approximately equal to or less than about 5° C.10. A pharmaceutical composition comprising an effective amount of clevidipine and Substance 23 claim 7 , wherein the ratio of the HPLC peak areas between clevidipine and Substance 23 is equal or above 500 to 1.11. A pharmaceutical composition comprising an effective amount of clevidipine and Substance 24 claim 7 , wherein the ratio of the HPLC peak areas between clevidipine and Substance 24 is equal or above 500 to 1.12. A pharmaceutical composition comprising an effective amount of clevidipine and Substance 25 claim 7 , wherein the ratio of the HPLC peak areas between clevidipine and Substance 23 is equal or above 500 to 1.14. The composition of claim 13 , wherein the amount of degradant is approximately equal to or less than about 1.0%.15. The composition of claim 13 , wherein the amount of degradant is approximately equal to or less than about 0.5%.17. The composition of claim 16 , wherein the amount of degradant is approximately equal to or less than about 1.0%.18. The composition of claim 16 , ...

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Номер записи: 72
02-01-2014 дата публикации

AUTOMATED ANALYZER

Номер: US20140005952A1
Автор: HASHIMOTO Yoshiaki, Okada Noriyoshi
Принадлежит: Beckman Coulter, Inc.

An analyzer, and a method and an apparatus for detecting liquid overflowing from a container that the analyzer comprises, are provided. The method according to the present invention for detecting liquid overflowing from a container in the analyzer, includes a step of judging that liquid is overflowing from at least one container if the difference between a standard deviation of absorbance of the liquid measured at a plurality of points of a container at time T and a standard deviation of absorbance of the liquid measured at a plurality of points in said container at time T is greater than a predetermined threshold value. 18.-. (canceled)9. A method for detecting liquid overflowing from at least one of a plurality of containers comprised in an analyzer , each container containing liquid , the method comprising the steps of: measuring an absorbance of the liquid contained in the one container at a plurality of points of the one container at a first time corresponding to the one container;', 'calculating a standard deviation of the absorbance of the liquid measured at the plurality of points of the one container at the first time corresponding to the one container, as a first standard deviation;', 'measuring an absorbance of the liquid contained in the one container at a second time corresponding to the one container;', 'calculating a standard deviation of the absorbance of the liquid measured at the plurality of points of the one container at the second time corresponding to the one container, as a second standard deviation; and', 'comparing a difference between the first standard deviation and the second standard deviation, with a predetermined threshold value; and, 'for each one of N-number of containers among the plurality of containers,'}judging that the liquid is overflowing from at least one of the plurality of containers if the difference between the first standard deviation and the second standard deviation is greater than the predetermined threshold value in ...

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Номер записи: 73
16-01-2014 дата публикации

CHROMATOGRAPHIC INTERFACE

Номер: US20140014585A1
Автор: Dourdeville Theodore A., Grigat Rolf, Phoebe Charles
Принадлежит: WATERS TECHNOLOGIES CORPORATION

An apparatus for chromatographic interfacing includes an interfacing unit, a sample acquisition unit, and a sample trapping unit. The interfacing unit includes (i) a chromatographic inflow valve adapted to receive a chromatographic sample separation flow, (ii) a chromatographic outflow valve adapted to allow discharge of the sample separation flow, (iii) a loop expulsion inflow valve adapted to receive an expulsion fluid flow, and (iv) a loop expulsion outflow valve in fluid communication with the loop expulsion inflow valve and adapted to allow discharge of a fluid. The sample acquisition unit includes (i) an inflow selector in selectable fluid communication with the chromatographic inflow valve or the loop expulsion inlet valve, (ii) an outflow selector in fluid communication with the loop expulsion outflow valve, and (iii) one or more collection loops, each loop being independently selectable to establish fluid communication between the inflow selector valve and the outflow selector valve. The sample trapping unit includes (i) a trap in selectable fluid communication with the loop expulsion outflow valve and defining a flow path from a first port to a second port and through a stationary phase disposed therebetween, the stationary phase adapted to trap an analyte of interest from a fraction of the chromatographic sample separation flow, and (ii) a scavenging gas source in selectable fluid communication with the flow path and a vacuum source in selectable fluid communication with the flow path, the scavenging gas source and vacuum source adapted to substantially dry the stationary phase. 1. An apparatus for chromatographic interfacing comprising: an interfacing unit including(i) a chromatographic inflow valve adapted to receive a chromatographic sample separation flow,(ii) a chromatographic outflow valve adapted to allow discharge of the sample separation flow,(iii) a loop expulsion inflow valve adapted to receive an expulsion fluid flow, and 'a sample acquisition ...

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Номер записи: 74
23-01-2014 дата публикации

TRACE ANALYTE EXTRACTION USING ADSORPTIVE CARBIDE-DERIVED NANOPOROUS CARBON POWDERS

Номер: US20140020447A1
Автор: Badorrek Christopher S., Burton Robert A., Kippeny Tadd C., Sengupta Louise C., Swafford Laura A.
Принадлежит: BAE Systems Information and Electronic Systems Integration Inc.

In the method of latent print analysis, wherein the improvement comprises using an ultra-fine, nanoporous carbon material as an adsorptive powder to identify trace chemicals found within oils of a print. 1. A method of chemical analyte analysis comprising the steps of utilizing a hyperadsorbent carbon material having a plurality of nanopores; collecting latent fingerprints; extracting analytes with a supercritical carbon dioxide solvent from a latent fingerprint; and analyzing analytes with liquid chromatography.2. The method of wherein said hyperadsorbent carbon material further comprises: a particle size of about 5 um; and a surface area of about 2500 m/g having a mean anopore diameter of about 5 nm.3. The method of wherein extracting analytes with a supercritical carbon dioxide solvent from a latent fingerprint further comprises utilizing a front end supercritical carbon dioxide extraction system.4. The method of wherein extracting analytes with a supercritical carbon dioxide solvent from a latent fingerprint further comprises extracting the analytes without disturbing said latent fingerprint.5. A method of forensic analysis comprising the steps of: utilizing a hyperadsorbent carbon material claim 2 , said hyperadsorbent carbon material having a mean nanopore diameter within the range of about 1 nm to about 5 nm;collecting a latent fingerprint; extracting analytes from a latent fingerprint; and analyzing analytes.6. The method of wherein said hyperadsorbent carbon material comprises a carbide-derived carbon skeleton and a functional group.7. The method of wherein the functional group is selected from the group consisting of metal carbide claim 6 , molybdenum claim 6 , silicon claim 6 , and/or titanium.8. The method of wherein said hyperadsorbent carbon material further comprises a particle size within the range of about 5 um to about 10 um; and a surface area of about 1 claim 7 ,000 m/g to about 2500 m/g having a mean nanopore diameter of 5 nm.9. The method of ...

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Номер записи: 75
23-01-2014 дата публикации

Systems and Methods for Measuring Total Sulfur Content in a Fluid Stream

Номер: US20140024129A1
Автор: Meyer Randy James, Meyer Shirley Ann, Smith Stevie Horton
Принадлежит: Cameron International Corporation

A system for determining the content of sulfur in a first fluid mixture includes a gas chromatograph including a sample valve, a first column coupled to the sample valve, and a second column coupled to the sample valve. In addition, the system includes a pyrolizer configured to subject the first fluid mixture to pyrolysis to produce a second fluid mixture that includes hydrogen sulfide. The first column is configured to separate at least a first constituent of the second fluid mixture from the hydrogen sulfide in the second fluid mixture and output a third fluid mixture including the hydrogen sulfide. The second column is configured to separate at least a second constituent in the third fluid mixture from the hydrogen sulfide in the third fluid mixture and output a fourth fluid mixture including the hydrogen sulfide. Further, the system includes a detector in fluid communication with the second column. 1. A system for determining the content of sulfur in a first fluid mixture including one or more sulfur compounds , the system comprising:a gas chromatograph including a sample valve configured to receive the first fluid mixture, a first column coupled to the sample valve, and a second column coupled to the sample valve;a pyrolizer coupled to the sample valve, wherein the pyrolizer is configured to subject the first fluid mixture to pyrolysis to produce a second fluid mixture that includes hydrogen sulfide;wherein the first column is configured to receive the second fluid mixture from the pyrolizer and separate at least a first constituent of the second fluid mixture from the hydrogen sulfide in the second fluid mixture and output a third fluid mixture including the hydrogen sulfide;wherein the second column is configured to receive the third fluid mixture from the first column and separate at least a second constituent in the third fluid mixture from the hydrogen sulfide in the third fluid mixture and output a fourth fluid mixture including the hydrogen sulfide; anda ...

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Номер записи: 76
30-01-2014 дата публикации

Optically heated analyte desorber for gas chromatography analysis

Номер: US20140026638A1
Автор: II Michael J. Bowers, Tadd C. Kippeny
Принадлежит: BAE Systems Information and Electronic Systems Integration Inc

Analytes are rapidly desorbed from a carbonaceous sorbent powder with improved quantitation and reduced analyte re-adsorption, thermal degradation, and rearrangement. The sample is distributed in a thin layer onto a desorption surface within a chamber. The layer can be a monolayer. Heating light irradiates the sample through a window, directly and rapidly heating the sample while the desorbed analytes diffuse into a vacuum or are removed by a carrier gas. Finally, the sorbent is flushed from the chamber by a transport gas. The desorption surface can be an inner surface of the window, or a surface of a porous frit that divides the chamber into two sections. The frit can be stainless steel or glass. The carrier gas can be helium, argon, or carbon dioxide. The light source can be a tungsten halogen lamp. A heater can control the chamber temperature according to a heating profile.

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Номер записи: 77
20-02-2014 дата публикации

Ex-Vivo Multi-Dimensional System For The Separation And Isolation Of Cells, Vesicles, Nanoparticles, And Biomarkers

Номер: US20140048417A1
Автор: Benjamin Sullivan, Dennis Carson, Michael Heller, Rajaram Krishnan, Sadik C. Esener
Принадлежит: UNIVERSITY OF CALIFORNIA

Devices and techniques are described that involve a combination of multidimensional electrokinetic, dielectrophoretic, electrophoretic and fluidic forces and effects for separating cells, nanovesicles, nanoparticulates and biomarkers (DNA, RNA, antibodies, proteins) in high conductance (ionic) strength biological samples and buffers. In disclosed embodiments, a combination of continuous and/or pulsed dielectrophoretic (DEP) forces, continuous and/or pulsed field DC electrophoretic forces, microelectrophoresis and controlled fluidics are utilized with arrays of electrodes. In particular, the use of chambered DEP devices and of a properly scaled relatively larger electrode array devices that combines fluid, electrophoretic and DEP forces enables both larger and/or clinically relevant volumes of blood, serum, plasma or other samples to be more directly, rapidly and efficiently analyzed. The invention enables the creation of “seamless” sample-to-answer diagnostic systems and devices. The devices and techniques described can also carry out the assisted self-assembly of molecules, polymers, nanocomponents and mesoscale entities into three dimensional higher order structures.

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Номер записи: 78
20-02-2014 дата публикации

Sample Component Trapping, Release, and Separation with Membrane Assemblies Interfaced to Electrospray Mass Spectrometry

Номер: US20140048700A1
Автор: White Thomas P., Whitehouse Craig M.
Принадлежит:

A method and apparatus to trap, release and/or separate sample components in solution passing through a channel with or without packing material present by passing ion current through the channel driven by an electric field. A portion of the ion current includes cation and/or anion species generated from second solution flows separated from the sample solution flow path by semipermeable membranes. Cation and/or anion species generated in the second solution flow regions are transferred into the sample solution flow path through ion selective semipermeable membranes. Ion current moving along the sample solution flow path is controlled by varying the composition of the second solutions and/or changing the voltage between membrane sections for a given sample solution composition. The sample composition may also be varied separately or in parallel to enhance trapping, release and/or separation efficiency and range. 1. (canceled)2. An apparatus for analyzing chemical species , comprising:a. at least one membrane assembly each configured with a semipermeable membrane separating a first flow channel and a second flow channel, and an electrode positioned in the second flow channel,b. a first solution flow and a second solution flow in the first and second flow channels, respectively,c. an ion source interfaced to a first solution flow outlet of the at least one membrane assembly, and a mass spectrometer,d. means for introducing sample species into the first solution, ande. a chromatography column or an electrically insulated open channel connected downstream of the first solution flow outlet.3. The apparatus according to claim 2 , further comprising a detector to detect the sample species eluting from the at least one membrane assembly.4. The apparatus according to claim 2 , wherein the ion source comprises an electrospray ion source.5. The apparatus according to claim 1 , further comprising means for applying and controlling voltages applied to the electrode.6. The ...

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Номер записи: 79
20-02-2014 дата публикации

BIOSENSOR AND BIOMATERIAL DETECTION APPARATUS INCLUDING THE SAME

Номер: US20140050621A1
Автор: Jang Won Ick, JEONG Eun-Ju, Kim Yark Yeon, LIM Ji Eun, YOON Yong Sun, Yu Han Young
Принадлежит:

Provided are a biosensor and a biomaterial detection apparatus including the same. The biomaterial detection apparatus comprises a light source to provide quantized photons; a substrate spaced apart from the light source; a single photonic sensor layer disposed on the substrate to sense the photons; and an adsorption layer disposed to cover the single photonic sensor layer, allow the photons to pass therethrough, and adsorb a biomaterial between the light source and the substrate. 1. A biomaterial detection apparatus comprising:a light source providing quantized photons;a substrate spaced apart from the light source;a single photonic sensor layer disposed on the substrate to sense the photons; andan adsorption layer covering the single photonic sensor layer, allowing the photons to pass therethrough, and adsorbing a biomaterial between the light source and the substrate.2. The biomaterial detection apparatus as set forth in claim 1 , wherein the single photonic sensor layer comprises an avalanche photodiode or a silicon photomultiplier.3. The biomaterial detection apparatus as set forth in claim 1 , wherein the adsorption layer comprises silicon or silicon oxide.4. The biomaterial detection apparatus as set forth in claim 1 , wherein the adsorption layer comprises at least one of glass claim 1 , quartz claim 1 , silicon nitride (SiN) claim 1 , germanium nitride (GeN) claim 1 , aluminum oxide (AlO) claim 1 , aluminum sulfide (AlS) claim 1 , gallium sulfide (GaS) claim 1 , indium sulfide (InS) claim 1 , aluminum selenide (AlSe) claim 1 , gallium selenide (GaSe) claim 1 , indium selenide (InSe) claim 1 , aluminum telluride (AlTe) claim 1 , gallium telluride (GaTe) claim 1 , indium telluride (InTe) claim 1 , aluminum cobalt (AlCO) claim 1 , polycarbonate claim 1 , poly(methyl methacrylate) (PMMA) claim 1 , and cyclic olefin copolymer (COC).5. The biomaterial detection apparatus as set forth in claim 1 , wherein the adsorption layer comprises a DNA adsorption layer.6. ...

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Номер записи: 80
27-02-2014 дата публикации

Fitting Assemblies

Номер: US20140053639A1
Автор: DellaRovere Dennis, Murphy James P.
Принадлежит: WATERS TECHNOLOGIES CORPORATION

A fitting assembly includes a first fitting that is configured to receive a first fluid tube and a second fitting that is configured to receive a second fluid tube. The first fitting defines a first groove. The second fitting includes a spring that is configured to engage the first groove to connect the first and second fittings such that the first and second fluid tubes are placed in fluid communication and a fluid tight seal is established between the first and second sealing fittings. 1. A fitting assembly comprising:a first fitting configured to receive a first fluid tube, the first fitting defining a first groove; 'a spring configured to engage the first groove to connect the first and second fittings such that the first and second fluid tubes are placed in fluid communication and a fluid tight seal is established between the first and second fittings.', 'a second fitting configured to receive a second fluid tube, the second fitting comprising2. The fitting assembly of claim 1 , wherein the first fitting defines a recess which terminates at a first sealing face claim 1 , and the second fitting defines a protrusion which terminates at a second sealing face claim 1 , and wherein the protrusion is sized to fit within the recess such that the first sealing face abuts against the second sealing face to form the fluid tight seal when the first and second fittings are connected.3. The fitting assembly of claim 1 , wherein the first fitting defines a recess claim 1 , and the second fitting defines a protrusion claim 1 , and wherein the recess and the protrusion have an interference fit to form the fluid tight seal when the first and second fittings are connected.4. The fitting assembly of claim 1 , wherein the second fitting comprises a fitting body defining a cylindrical bore claim 1 , and wherein the first fitting comprises a tubular body configured to be at least partially inserted into the cylindrical bore to establish a connection between the first and second ...

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Номер записи: 81
06-03-2014 дата публикации

Gas chromatograph data processing device, recording medium recording data processing program, and data processing method

Номер: US20140067304A1
Автор: Tomohiro Sasaki
Принадлежит: Horiba Stec Co Ltd

Provided in order to permit accurate calculation of an adjusted retention time of alkane at a arbitrary temperature and thus permit more improvement in accuracy in calculation of a retention index at the arbitrary temperature based on a known retention index is a column inlet pressure setting part which receives at least a second sample temperature and outputs a second column inlet pressure such that a void time as a retention time of a non-retained substance as a substance not retained in a column at the second sample temperature becomes substantially equal to a void time at a first sample temperature.

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Номер записи: 82
27-03-2014 дата публикации

METHOD FOR DETECTING URINE ODOR FROM AIR CONDITIONER, REPRODUCING URINE ODOR AND PREPARING CORRESPONDING URINE ODOR COMPOSITION

Номер: US20140087471A1
Автор: KIM Ji Wan, Kim Seok Man, Lee Tae Hee
Принадлежит: HYUNDAI MOTOR COMPANY

Disclosed herein is a method for identifying the compounds contributing to urine odor emitting from an air conditioner, a method for artificially reproducing the detected urine odor, and preparing a corresponding urine odor composition. Through the analysis method of the present invention, the compounds contributing to the urine odor emitted from an air conditioner may be identified and quantified. The detected urine odor may be reproduced from a combination of the compounds identified by the analysis method of the present invention. The reproduced urine odor may provide meaningful data required for development of an apparatus and a method for removing specific odor. 1. A detected urine odor composition from an air conditioner comprising one or more compounds selected from a group consisting of: acetaldehyde , butyraldehyde , toluene , o-xylene , m-xylene , p-xylene , methyl ethyl ketone , methyl isobutyl ketone and butyl acetate.2. A detected urine odor composition from an air conditioner comprising: acetaldehyde , butyraldehyde , toluene , o-xylene , m-xylene , p-xylene , methyl ethyl ketone , methyl isobutyl ketone and butyl acetate.3. The detected urine odor composition from an air conditioner according to claim 2 , further comprising:0.0015-0.15 ppm of acetaldehyde;0.0006-0.050 ppm of butyraldehyde;0.03-25 ppm of toluene;0.002-2.9 ppm of o-xylene;0.003-2.7 ppm of m-xylene;0.003-2.7 ppm of p-xylene;0.004-35 ppm of methyl ethyl ketone;0.0005-28 ppm of methyl isobutyl ketone; and0.0005-38 ppm of butyl acetate.4. The detected urine odor composition from an air conditioner according to claim 2 , further comprising one or more compounds selected from a group consisting of: hexanal claim 2 , heptane claim 2 , methylcyclopentane claim 2 , nonane claim 2 , n-octane claim 2 , n-pentane and n-undecane.5. The detected urine odor composition from an air conditioner according to claim 4 , further comprising:0.00001-0.01 ppm of hexanal;0.00015-0.15 ppm of heptane;0.00006-0.06 ...

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Номер записи: 83
27-03-2014 дата публикации

METHOD FOR DETECTING SOUR ODOR FROM AIR CONDITIONER, REPRODUCING SOUR ODOR AND PREPARING CORRESPONDING SOUR ODOR COMPOSITION

Номер: US20140087475A1
Автор: KIM Ji Wan, Lee Tae Hee
Принадлежит: HYUNDAI MOTOR COMPANY

Disclosed herein is a method for identifying compounds contributing to a sour odor emitting from an air conditioner, a method for artificially reproducing the detected sour odor and preparing a corresponding sour odor composition. Through the analysis method of the present invention, the compounds contributing to the sour odor emitted from an air conditioner may be identified and quantified. The detected sour odor may be reproduced from a combination of the compounds identified by the analysis method of the present invention. The reproduced sour odor may provide meaningful data required for development of an apparatus and a method for removing specific odors. 1. A detected sour odor composition from an air conditioner comprising one or more compounds selected from a group consisting of ammonia , acetaldehyde , propionaldehyde , n-butyraldehyde , toluene , xylene and methyl ethyl ketone.2. A detected sour odor composition from an air conditioner comprising ammonia , acetaldehyde , propionaldehyde , n-butyraldehyde , toluene , xylene and methyl ethyl ketone.3. The detected sour odor composition from an air conditioner according to claim 1 , further comprising:0.1-2 ppm of ammonia;0.002-0.1 ppm of acetaldehyde;0.0001-0.1 ppm of propionaldehyde;0.0003-0.03 ppm of n-butyraldehyde;0.03-30 ppm of toluene;0.001-2 ppm of xylene; and0.003-7 ppm of methyl ethyl ketone.4. The detected sour odor composition from an air conditioner according to claim 2 , further comprising one or more compounds selected from a group consisting of formaldehyde claim 2 , hexane claim 2 , N-phenylbenzenamine claim 2 , phenol claim 2 , 2-phenyl-2propanol claim 2 , benzene and benzothiazole.5. The detected sour odor composition from an air conditioner according to claim 4 , further comprising:0.00002-0.009 ppm of formaldehyde;0.007-0.04 ppm of hexane;0.001-0.4 ppm of N-phenylbenzenamine;0.003-0.6 ppm of phenol;0.001-0.2 ppm of 2-phenyl-2propanol;0.001-0.2 ppm of benzene; and0.001-0.1 ppm of ...

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Номер записи: 84
03-04-2014 дата публикации

PART FOR A COMPONENT FOR HIGH-PRESSURE LIQUID CHROMATOGRAPHY (HPLC), IN PARTICULAR PUMP HEAD FOR AN HPLC PUMP, AND ALSO HPLC PUMP

Номер: US20140093418A1
Автор: SATZINGER Adolf, Schloderer Richard, Seitz Stefan Andreas
Принадлежит: DIONEX SOFTRON GMBH

The invention relates to a part for a component for high-pressure liquid chromatography (HPLC), in particular a pump head for an HPLC pump, in which the strength has been increased by autofrettage and which consists of a material which is essentially chemically inert to the fluids used in HPLC. The invention further relates to an HPLC pump having a pump head which is configured as such a part. 1. A part for a component for high-pressure liquid chromatography (HPLC) comprising: a material of the part for HPLC that is strengthened by autofrettage , and in that the material is essentially chemically inert to fluids used in HPLC.2. The part of claim 1 , in which the material comprises a titanium alloy.3. The part of claim 2 , in which the titanium alloy comprises at least 75% by weight titanium and a proportion of at least 7% by weight of one or more alloying substances selected from the group consisting of aluminum claim 2 , vanadium claim 2 , niobium claim 2 , zirconium claim 2 , chromium or molybdenum.4. The part of claim 3 , in which the proportion of the alloying substances is at least 10% by weight.5. The part of claim 3 , in which the proportion of titanium is at leasst 88% by weight.6. The part of claim 1 , in which the part includes a bore intersection.7. The part of claim 1 , in which the part comprises a pump head for an HPLC pump.8. A pump for HPLC comprising the pump of .9. The part of claim 1 , in which the part is selected from a group consisting of a capillary claim 1 , a switching valve claim 1 , a column claim 1 , and a valve.10. The part of claim 7 , in which the pump head includes a region of a plasticized inner wall from exposure to a pressure above a yield strength of the material. The present invention relates to a part for a component for high-pressure liquid chromatography (HPLC), in particular a pump head for an HPLC pump, and also to an HPLC pump having such a part.In HPLC, the use of fine-grained column material can achieve a desired ...

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Номер записи: 85
10-04-2014 дата публикации

Apparatuses and Methods for Wireless Monitoring and Control of Environmental Sampling and Chromatographic Apparatuses

Номер: US20140096597A1
Автор: Curran Jon L., Davison Dale A., Jameson Daniel G., Meyer Dale L., Pipes Ruth A., Silver Jack E.
Принадлежит: Teledyne Instruments, Inc.

A liquid chromatographic system includes columns, column mounting fixtures to which the columns are mounted, a detector, a collector, a controller and a plurality of RFIDs. A first RFID communicates with the controller and cooperating RFIDs mounted to other components provide information such as the history of components, parameters and the like. They also receive information from sensors relating to the operation of the liquid chromatograph, store the information and transmit it. Moreover, the RFIDs may substitute for hard wiring in many applications and may enable a central computer to control several liquid chromatographic and environmental sample collectors. 1. A portable instrument comprising:at least one chromatographic column; said at least one chromatographic column having a plurality of sensors spaced along its length;at least one wireless communication device on said portable instrument;at least some of said plurality of sensors being configured to communicate with the at least one wireless communication device;an input for the chromatographic column;a readout device coupled to said portable instrument, said readout device operably connected to said wireless communication device, wherein readings from the plurality of sensors may be displayed.2. A portable instrument in accordance with further including a positioning device configured to provide a location of an operation of said portable instrument; said positioning system being able to record a position of said at least one chromatographic column.3. A portable instrument in accordance with in which said positioning device is a global positioning system.4. A portable instrument comprising:a sample collector for collecting a plurality of liquid samples, said sample collector configured for receiving a plurality of sizes of containers;at least one container of said plurality of sizes of containers having a mounted nonvolatile memory, said mounted nonvolatile memory configured for receiving and storing ...

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Номер записи: 86
10-04-2014 дата публикации

Method and apparatus for determination of haloacetic acid ("HAA") presence in aqueous solution

Номер: US20140099727A1
Автор: Saini Harmesh K.
Принадлежит:

Techniques related to printing using a metal-surface charging element. A printing system includes a metal-surface charging element and a power supply. The charging element is disposed to deposit electric charge on an imaging surface. The power supply may provide electric power with an alternating current (AC) component and a direct current (DC) component to the charging element. 1. A method of determining concentration of haloacetic acid present in an aqueous solution , comprising:extracting haloacetic acid from the aqueous solution onto an adsorbent medium;displacing the haloacetic acid from the adsorbent medium to concentration medium;eluting the haloacetic acid from the concentration medium and transferring the eluted haloacetic acid to a HPLC column;isolating the haloacetic acid based upon a retention time in the HPLC column; anddetermining concentration of the haloacetic acid based on UV-absorbance measurement.2. The method of claim 1 , embodied as a method of determining concentration of plural haloacetic acid species claim 1 , where:isolating includes isolating each of the plural haloacetic acid species based upon respective retention times; anddetermining concentration includes determining concentration of each of the plural haloacetic acid species based on respective UV-absorbance measurement.3. The method of claim 1 , where:the aqueous solution includes water and the method further comprises extracting a sample volume of waterextracting haloacetic acid from the aqueous solution onto the absorbent medium includes passing the haloacetic acid through a column packed with ion-exchange adsorbent medium to extract the haloacetic acid; andextracting the haloacetic acid from the aqueous solution includes water47-. (canceled)8. The method of claim 1 , where eluting includes passing an elution solution through the concentration medium claim 1 , the elution solution selected to be an alkaline solution capable of deprotonising the haloacetic acid to disrupt it-it ...

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Номер записи: 87
01-01-2015 дата публикации

AUTOMATIC SAMPLE POURING DEVICE

Номер: US20150000386A1
Автор: Minato Hiroyuki
Принадлежит: SHIMADZU CORPORATION

An automatic sample pouring device comprising a rack, a sampling needle, a reference portion arranged at a predetermined position of the rack, a detection unit for electrically detecting contact between the reference portion and the needle, and a control unit for controlling an operation of relatively moving the needle on a horizontal plane and in a vertical direction with respect to the rack, and suctioning and pouring operations of a sample. The control unit is configured to control an operation of obtaining reference position information of the rack on the basis of detection information of the detection unit. 15.-. (canceled)6. An automatic sample pouring device comprising:a rack allowing mounting of a plurality of sample containers;a sampling needle for suctioning samples contained in the sample containers;a reference portion, arranged at a predetermined position of the rack, including a concave portion of a predetermined depth, a shape of an inner wall surface of the reference portion in horizontal cross section being point-symmetric;a detection unit for electrically detecting contact between the reference portion and the needle; anda control unit for controlling an operation of relatively moving the needle on a horizontal plane and in a vertical direction with respect to the rack, an operation of obtaining reference position information of the rack on the basis of detection information of the detection unit, and suctioning and pouring operations of a sample.7. The automatic sample pouring device according to claim 6 ,wherein, after moving a tip end of the needle into the reference portion, the control unit moves, until contact between the reference portion and the needle is detected on the basis of detection information of the detection unit, the needle in both positive and negative directions in an X direction on the horizontal plane, both positive and negative directions in a Y direction that is orthogonal to the X direction on the horizontal plane, and a Z ...

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Номер записи: 88
04-01-2018 дата публикации

DECARBOXYLATED CANNABIS RESINS, USES THEREOF AND METHODS OF MAKING SAME

Номер: US20180000857A1
Автор: Grover Har, Kotra Lakshmi Premakanth, Lewis Melissa Maureen, Wasilewski Ewa
Принадлежит:

The disclosure relates to decarboxylated resins and methods of making the decarboxylated resins by extraction and decarboxylation of cannabinoids from species using microwaves and solvents. The disclosure also relates to use of the decarboxylated resins for making pharmaceutical products comprising same. 1cannabisCannabis. A decarboxylated resin , wherein the resin comprises decarboxylated cannabinoids from a spp. plant , such that Δ9-tetrahydrocannabidiolic acid (Δ9-THCA) and cannabidiolic acid (CBDA) cannabinoids in the plant are each independently 90% to 100% decarboxylated to yield Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) respectively in the resin.2cannabis. The decarboxylated resin of claim 1 , wherein the resin further comprises cannabinol (CBN).3cannabis. The decarboxylated resin of claim 1 , where the resin is substantially free of solvent.4cannabis. A pharmaceutical product comprising (i) the decarboxylated resin of and (ii) a suitable pharmaceutically acceptable carrier or excipient.5cannabis. A natural health product comprising the decarboxylated resin of .6cannabis. A method of extracting and decarboxylating cannabinoids from plant material claim 1 , the method comprising:{'i': cannabis', 'cannabis', 'cannabis', 'cannabis, '(i) extracting cannabinoids from the plant material by contacting the plant material with a solvent to extract cannabinoids from the plant material, the extract of comprising the solvent and cannabinoids; and'}(ii) decarboxylating the cannabinoids in (a) the plant material and (b) the extract comprising cannabinoids by subjecting the plant material and extract to microwaves at temperature of about 100-200° C. in a sealed container or sealed vessel and for a time period sufficient to form the corresponding decarboxylated cannabinoids in the extract.7cannabis. The method of claim 6 , wherein before the step of extracting claim 6 , the plant material is broken down to form plant material of a size and form suitable for ...

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Номер записи: 89
04-01-2018 дата публикации

METAL OXIDE-BASED BIOCOMPATIBLE HYBRID SORBENT FOR THE EXTRACTION AND ENRICHMENT OF CATECHOLAMINE NEUROTRANSMITTERS AND RELATED COMPOUNDS, AND METHOD OF SYNTHESIS

Номер: US20180001298A1
Автор: ALHENDAL ABDULLAH AWADH, Malik Abdul
Принадлежит: UNIVERSITY OF SOUTH FLORIDA

The subject invention concerns metal or metalloid oxide-based sol-gel hybrid sorbent and methods of synthesis. In one embodiment, the sorbent is a ZrOpolypropylene oxide based sol-gel. The subject invention also concerns a hollow tube or capillary internally coated with a sorbent of the invention. Sorbent coated tubes and capillaries of the invention can be used in extraction and/or enrichment of samples to be analyzed for catecholamines and related compounds. 1. A metal or metalloid oxide-based sol-gel hybrid sorbent composition prepared from a biocompatible polymer or ligand comprising one or more sol-gel active end groups.2. The sorbent composition according to claim 1 , wherein the metal or metalloid of the sorbent composition is aluminum claim 1 , antimony claim 1 , arsenic claim 1 , barium claim 1 , beryllium claim 1 , bismuth claim 1 , boron claim 1 , cadmium claim 1 , cerium claim 1 , chromium claim 1 , cobalt claim 1 , copper claim 1 , dysprosium claim 1 , erbium claim 1 , europium claim 1 , gadolinium claim 1 , gallium claim 1 , gold claim 1 , hafnium claim 1 , holmium claim 1 , indium claim 1 , iridium claim 1 , iron claim 1 , lanthanum claim 1 , lithium claim 1 , magnesium claim 1 , manganese claim 1 , molybdenum claim 1 , neodymium claim 1 , nickel claim 1 , niobium claim 1 , osmium claim 1 , palladium claim 1 , platinum claim 1 , praseodymium claim 1 , rhodium claim 1 , ruthenium claim 1 , samarium claim 1 , scandium claim 1 , selenium claim 1 , silver claim 1 , strontium claim 1 , tellurium claim 1 , terbium claim 1 , thallium claim 1 , thulium titanium claim 1 , tantalum claim 1 , vanadium claim 1 , yttrium claim 1 , zirconium claim 1 , zinc claim 1 , tungsten claim 1 , or any combination thereof.3. The sorbent composition according to claim 1 , wherein the sorbent composition comprises ZrOpolypropylene oxide (ZrOPPO).5. The sorbent composition according to claim 1 , wherein the polymer or ligand used to prepare the sorbent composition comprises one ...

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Номер записи: 90
03-01-2019 дата публикации

Stationary phase for supercritical fluid chromatography

Номер: US20190001238A1
Автор: Satoshi Shinkura, Toru Shibata
Принадлежит: Daicel Corp

Provided is a stationary phase for supercritical fluid chromatography that includes a carrier on which is supported a polymer that includes a pyrrolidone backbone in the repeating units of the main chain.

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Номер записи: 91
02-01-2020 дата публикации

NOVEL COMPOUND, FLUORESCENCE DERIVATIZATION REAGENT INCLUDING SAID NOVEL COMPOUND, METHOD FOR OPTICALLY RESOLVING OPTICAL ISOMER OF AMINO ACID IN WHICH SAID NOVEL COMPOUND IS USED, AND FLUORESCENCE DERIVATIZED AMINO ACID

Номер: US20200002322A1
Автор: AKITA Takeyuki, HAMASE Kenji, MITA Masashi
Принадлежит: SHISEIDO COMPANY, LTD.

The object of the present invention is to develop a reagent for optical resolution for the analysis of chiral amino acids wherein quenching is not exhibited. This object is achieved by providing a novel compound for optical resolution wherein quenching is not exhibited. The present invention relates to a novel compound, a reagent for optical resolution comprising the novel compound, a method for optically resolution comprising a step of reacting the novel compound, and optical isomers obtained by reacting the novel compound with amino acids. 4. The method according to claim 3 , wherein the method of analysis is a method of optically resolving optical isomers and the chromatography is chiral chromatography.5. The method according to or wherein the amino group-containing compound is an amino acid. The present invention relates to a novel compound, a regent for fluorescence derivatization comprising the novel compound, a method for optically resolving fluorescently derivatized amino acids obtained by reacting the novel compound with amino acids, and the fluorescently derivatized amino acids obtained by reacting the novel compound with amino acids.Conventionally, amino acids present in vivo in higher animals were thought to be only L-amino acids. However, recently, due to advances in analytical techniques, it has become clear that D-amino acids are also present in vivo in higher animals, particularly in mammals including humans, and are involved in physiological functions such as memory and learning as well as hormone production and secretion. Furthermore, some of the D-amino acids present in various tissues and biological samples have been shown to fluctuate depending on the condition of the body, for example, due to disorders such as kidney failure. Thus, the use thereof as health and disease markers are anticipated (Patent Literature 1).For the purpose of elucidating the physiological roles of amino acids in vivo, advances are being made in developmental research ...

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Номер записи: 92
03-01-2019 дата публикации

METHODS OF PRODUCING AND CHARACTERIZING VIRUS VACCINE AND VIRUS VACCINE COMPOSITION

Номер: US20190002839A1
Автор: AI Wen, LIU Dianlian, SHEN Mingfeng
Принадлежит:

This application pertains to methods of isolating virus particles and producing virus vaccine composition comprising subject a biological sample to an anion exchange chromatography and a hydroxyapatite chromatography. The application also pertains to rabies virus vaccine compositions and methods of assessing suitability of a virus vaccine composition or releasing a commercial batch of virus vaccine composition for clinical use. 1. A method of purifying rabies virus from a biological sample , comprising subjecting the biological sample to an anion exchange (“AE”) chromatography followed by a hydroxyapatite (“HA”) chromatography.2. The method of claim 1 , wherein the AE chromatography comprises:a) an AE loading step, comprising loading the biological sample to an AE column; andb) an AE elution step, comprising eluting the AE column with an AE elution buffer.3. The method of claim 1 , wherein the HA chromatography comprises:a) an HA loading step, comprising loading a post-AE chromatography sample to an HA column; ande) an HA elution step, comprising eluting the HA column with an HA elution buffer.4. The method of claim 1 , wherein the anion exchange chromatography is Capto-DEAE chromatography.5. The method of claim 1 , wherein the hydroxyapatite chromatography is CHT chromatography.6. The method of claim 2 , wherein the AE chromatography further comprises a second AE elution step comprising eluting the AE column with a second AE elution buffer claim 2 , wherein a first AE eluate and a second AE eluate are collected from the first AE elution step and the second AE elution step claim 2 , respectively claim 2 , and wherein the first AE eluate and the second AE eluate comprise virus with different structure claim 2 , purity or virus protein composition.7. The method of claim 2 , wherein the AE chromatography further comprises:a) an AE pre-equilibration step comprising pre-equilibrating the anion exchange column with an AE pre-equilibrating buffer;b) an AE equilibrating ...

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Номер записи: 93
07-01-2016 дата публикации

SOLUTE EXTRACTING APPARATUS

Номер: US20160003784A1
Автор: FUJITA Hiroyuki, Nakamura Hirofumi
Принадлежит: MIURA CO., LTD.

A solute trapping column including two trapping agent layers has a first branched pathway extending from an upper part of a main body part thereof, and a second branched pathway extending from between two trapping agent layers, and with its upper part a purification column is connected. By injection of solution of dioxins and supply of solvent into the purification column, dioxins are trapped by the trapping agent layers. An extraction solvent supplied from the lower end side of the solute trapping column while keeping both the purification column and the first branched pathway blocked extracts the dioxins trapped by the second trapping agent layer and flows into the second branched pathway. An extraction solvent supplied in a similar manner while keeping both the purification column and the second branched pathway blocked extracts the dioxins trapped by the first trapping agent layer and flows into the first branched pathway. 1. An apparatus for extracting a solute from a solution , comprising:a solute trapping column having an injection part equipped with an injection port for the solution at one end and a discharge port at the other end, and including multiple trapping agent layers capable of trapping the solute packed with a space interposed therebetween;a first supplying device for supplying the solute trapping column with a solvent for developing the solute within the solute trapping column; anda second supplying device for supplying the solute trapping column with a solvent for extracting the solute,wherein, the solute trapping column has branched pathways, which are independent of each other and respectively extend from the injection part and the space, for the flow of the extraction solvent from the second supplying device, andthe second supplying device is settable during its operation so that the extraction solvent flows in one of the branched pathways sequentially selected from the extraction solvent supply side while flowing of the extraction solvent in ...

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Номер записи: 94
02-01-2020 дата публикации

Dexrazoxane analytical method

Номер: US20200003737A1
Автор: Changjun FAN, Hongyu Chen, Min Sun, Shuaihua TIAN, Xiangfeng CHEN
Принадлежит: Jiangsu Aosaikang Pharmaceutical Co Ltd

A high performance liquid chromatography method used for dexrazoxane-related substances is provided, and in the method, a low-density bonding reversed-phase C18 chromatographic column resistant to pure water is employed; a gradient elution is carried out with mobile phase A and mobile phase B as eluents, the mobile phase A being a buffer, and the mobile phase B being an organic solvent; the volume percent of mobile phase A in eluents in a first stage of the gradient elution is not lower than 90%, and the duration of the first stage of the gradient elution ranges from 15˜30 minutes. By means of the analytical method, dexrazoxane is effectively separated from main impurities, and the qualities of the active pharmaceutical ingredients of dexrazoxane and the preparations thereof could be better controlled.

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Номер записи: 95
07-01-2021 дата публикации

METHOD FOR ANALYSING PROCESS STREAMS

Номер: US20210003502A1
Автор: HAUBER Christoph, KIRCHMANN Marius
Принадлежит:

The invention relates to a method for investigating process streams comprising five or more different hydrocarbon-containing components. In the method at least one process flow line () is in operative connection with an online IR spectrometer () and an online gas chromatograph (). The process stream passed through the process stream conduit () is subjected to an online characterization which comprises measurements both with the online IR spectrometer and with an online gas chromatograph. The spectral data and the chromatography data are mathematically related to one another by suitable statistical models, thus allowing training of a model used for evaluating the analytical data and for characterizing the process streams. The method according to the invention is characterized by short measurement times in the range of seconds and milliseconds and a high accuracy. The method according to the invention for investigating process streams preferably relates to investigation of process streams deriving from processes proceeding in parallel, the process streams preferably deriving from reaction spaces arranged in parallel. 1. A method for investigating at least one process stream comprising at least five different hydrocarbon-containing components , wherein the method comprises at least the steps of:{'b': 35', '2', '1, 'a) providing at least one process stream conduit () which is in operative connection with at least one online IR spectrometer () and in operative connection with at least one online gas chromatograph ();'}{'b': 35', '35', '2', '1, 'b) passing at least one process stream through the at least one process stream conduit (), wherein during this passing of the process stream through the process stream conduit () an analytical characterization of the process stream using an online IR spectrometer () and an online gas chromatograph () is performed.'}2. The method according to claim 1 , further comprising the steps of:{'b': '2', 'c) evaluating the spectral data ...

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Номер записи: 96
07-01-2021 дата публикации

ANALYSIS METHOD OF 3-METHYL-1-PHENYL-2-PYRAZOLIN-5-ONE BULK DRUG, TREATMENT FOR AMYOTROPHIC LATERAL SCLEROSIS, INHIBITION OF PROGRESSION OF AMYOTROPHIC LATERAL SCLEROSIS, AND METHOD OF PRODUCING DRUG CONTAINING 3-METHYL-1-PHENYL-2-PYRAZOLIN-5-ONE BULK DRUG

Номер: US20210003544A1
Автор: SANO Aya, WAKASUGI Takeshi
Принадлежит: MITSUBISHI TANABE PHARMA CORPORATION

This method of analyzing the phenylhydrazine content in a 3-methyl-1-phenyl-2-pyrazolin-5-one bulk drug involves: obtaining a first measured value by measuring the phenylhydrazine content of a standard solution that contains phenylhydrazine or a salt thereof, a first acidic water and a first water-soluble organic solvent, and that exhibits a phenylhydrazine concentration of 0.01 μg/mL to 10 μg/mL; obtaining a second measured value by measuring the phenylhydrazine content in a sample solution that contains a 3-methyl-1-phenyl-2-pyrazolin-5-one bulk drug, a second acidic water and a second water-soluble organic solvent; and detecting the phenylhydrazine content in the 3-methyl-1-phenyl-2-pyrazolin-5-one bulk drug on the basis of the first measured value and the second measured value. The first acidic water is at least one type selected from hydrochloric acid, an aqueous acetic acid solution, and the like, the first water-soluble organic solvent is at least one type selected from acetonitrile and methanol, the second acidic water is at least one type selected from hydrochloric acid, an aqueous acetic acid solution, and the like, and the second water-soluble organic solvent is at least one type selected from acetonitrile, and methanol. 1. A method of analyzing the phenylhydrazine content in a 3-methyl-1-phenyl-2-pyrazolin-5-one bulk drug , which comprises:obtaining a first measured value by measuring the phenylhydrazine content in a phenylhydrazine standard solution that contains phenylhydrazine or a salt thereof, a first acidic water and a first water-soluble organic solvent, and that exhibits a phenylhydrazine concentration of 0.01 μg/mL to 10 μg/mL,obtaining a second measured value by measuring the phenylhydrazine content in a 3-methyl-1-phenyl-2-pyrazolin-5-one sample solution that contains a 3-methyl-1-phenyl-2-pyrazolin-5-one bulk drug, a second acidic water and a second water-soluble organic solvent, anddetecting the phenylhydrazine content in the 3-methyl-1- ...

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Номер записи: 97
07-01-2021 дата публикации

METHODS FOR EXTRACTING PROTEINS FROM BLOOD PLASMA

Номер: US20210003552A1
Автор: Curtin Dennis, Zurlo Eugene
Принадлежит:

A method of producing protein products including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins from plasma includes steps of: (1) adding a salt to the blood product to produce a first intermediate, wherein the salt comprises between 11-13 wt % of the first intermediate; (2) separating the first intermediate to produce a first supernatant and a first paste; (3) adding a salt to the first intermediate to produce a second intermediate, wherein the salt comprises between 21-23 wt % of the second intermediate; (4) separating the second intermediate to produce a second supernatant and a second paste; (5) separating a third intermediate from the second supernatant by affinity chromatography; and (6) separating the third intermediate by ion exchange chromatography to produce an eluate containing the protein product. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields. 1. A method of separating A1P1 from a blood plasma containing product , the method comprising:thawing a frozen blood plasma product followed by stirring at a temperature suitable for dissolving a cryoprecipitate to generate the blood plasma containing product;adding a salt to the blood plasma containing product to produce a first intermediate, wherein the salt comprises between 11-20 wt % of the first intermediate;separating the first intermediate to produce a first supernatant and a first paste;adding a salt to the first supernatant to produce a second intermediate, wherein the salt comprises between 15-30 wt % of the second intermediate;separating the second intermediate to produce a second supernatant and a second paste;separating the second supernatant by affinity chromatography using an A1P1-specific affinity media to generate a flow-through fraction and a first eluate, wherein the first eluate comprises A1P1.2. The method of claim 1 , wherein the step of adding the salt to the ...

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Номер записи: 98
07-01-2021 дата публикации

METHOD

Номер: US20210003585A1
Автор: JENEY Csaba
Принадлежит:

The present invention relates generally methods and kits for detecting binding interactions, in particular protein-protein interactions, and particularly to high throughput methods for labelling, analysing, detecting and measuring protein-protein interactions. 1. A method of determining a binding interaction between a binding agent and a target comprisinga) contacting a binding agent library with a target to allow formation of binding agent/target complex, wherein each member of said binding agent library is associated with a unique nucleotide sequence and wherein said target is associated with a unique nucleotide sequence;b) isolating said binding agent/target complexes into compartments so that there is a single binding/agent complex in one compartment;c) linking the unique nucleotide sequence(s) associated with the binding agent and target in the binding agent/target complex to form a linked nucleotide sequence, wherein isolation of the isolated binding agent/target complexes is maintained during the linking step;d) identifying the binding agent(s) present in the complex from the linked nucleotide sequence; ande) using the linked nucleotide sequence correlating the unique nucleotide sequence of each member of said binding agent library with the binding characteristics of said member.2. The method as claimed in wherein said binding agent library comprises an antibody library.3. The method of wherein said antibody library comprises an antibody display library or a library of antibodies wherein each antibody is labelled with said unique nucleotide sequence.4. (canceled)5. The method of wherein said target comprises a protein.6. The method of wherein said target comprises a protein display library claim 1 , herein each member of said library is associated with a unique nucleotide sequence.7. (canceled)8. The method of claim 5 , wherein said protein is within a protein mixture or an enriched protein mixture.9. (canceled)10. The method of claim 8 , wherein said protein ...

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Номер записи: 99
07-01-2021 дата публикации

Method for improving monoclonal antibody detection results

Номер: US20210003587A1
Автор: Noriko Iwamoto, Takashi Shimada
Принадлежит: Shimadzu Corp

The present invention provides a detection method for a monoclonal antibody in a sample, comprising: (a) a step of capturing the monoclonal antibody in the sample and immobilizing the monoclonal antibody in pores of a porous body; (b) a step of bringing the porous body in which the monoclonal antibody is immobilized with nanoparticles on which protease is immobilized to conduct selective protease digestion of the monoclonal antibody; (c) a step of detecting, by a liquid chromatography mass spectrometry (LC-MS), peptide fragments obtained by the selective protease digestion; and (a′) a step of conducting a reduction reaction under an acidic condition after the step (a). By the present invention, further applicability of the detection method for the monoclonal antibodies using mass spectrometry is expected.

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Номер записи: 100