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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 9834. Отображено 100.
05-01-2012 дата публикации

Minute particle analyzing device and method

Номер: US20120001090A1
Принадлежит: Icyt Mission Technology Inc, Sony Corp

A minute particle analyzing device includes: a light source; a first condenser lens for condensing light from the light source to a first end of a multimode optical fiber; a second condenser lens for condensing the light emerging from a second end of the multimode optical fiber to a minute particle; and a detector for detecting light generated from the minute particle by the application of the light from the light source.

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05-01-2012 дата публикации

Apparatus, System, and Method For Increasing Measurement Accuracy in a Particle Imaging Device Using Light Distribution

Номер: US20120002040A1
Принадлежит: Luminex Corp

An apparatus, system, and method for increasing measurement accuracy in imaging cytometry. The system may include a light detector configured to measure light emitted by a particle in response to a first light source, and processor coupled to the light detector. The processor may be configured create a first image by taking a first measurement of light and create a second image by interpolating the first image, where the second image has higher resolution than the first image. The processor may be configured to determine a difference between pixels of the second image and an expected distribution and discard the first measurement of light if the difference is above a predetermined threshold.

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02-02-2012 дата публикации

Fluorescence detection device and fluorescence detection method

Номер: US20120025098A1

When receiving fluorescence emitted by a measurement object irradiated with laser light emitted from a laser light source unit, a fluorescence detection device generates a modulation signal for modulating the intensity of the laser light and modulates the laser light using the modulation signal. The fluorescence detection device obtains a fluorescent signal of the fluorescence emitted by the measurement object irradiated with the laser light, and calculates, from the fluorescent signal, a fluorescence intensity and the phase delay of the fluorescence with respect to the modulation signal. At the time, the fluorescence detection device controls the operation amounts of the signal level of a DC component of the modulation signal and the gain of amplification just after the output of the fluorescent signal so that the value of a fluorescence intensity signal falls within a preset range. After the operation amounts are settled, the fluorescence detection device calculates the fluorescence intensity and then calculates the fluorescence relaxation time of the fluorescence emitted by the measurement object using the phase delay.

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09-02-2012 дата публикации

Method and apparatus for multi-parameter data analysis

Номер: US20120035859A1
Автор: Nicholas Thomas
Принадлежит: GE Healthcare UK Ltd

In one aspect, the present invention relates to a method 200 for identifying one or more phenotypes from a multi-parameter data set. The method 200 comprises measuring 202 correlation between pairs of parameters within the multi-parameter data set, modifying 204 correlated parameter values within a predetermined multi-parameter data analysis set to form an analysis parameter set, and analysing 206 the multi-parameter data set using the analysis parameter set to identify one or more phenotypes from the multi-parameter data set. Various embodiments of the present invention may, for example, be used in an automated high-content screening (HCS) apparatus 100 for biological cellular analysis.

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15-03-2012 дата публикации

Single-cell microchamber array

Номер: US20120065082A1

An apparatus for analyzing individual cell composition in a heterogeneous cell population may include, in one embodiment, a deposition plate having an array of microwells disposed therein, and a cover plate substantially overlying the deposition plate. A pair of electrodes may be associated with one or more of the microwells, and may be configured to generate an electric field within the associated microwell.

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10-05-2012 дата публикации

High fidelity colour imaging of microbial colonies

Номер: US20120114218A1
Принадлежит: SYNOPTICS Ltd

A system and an associated method of imaging microbial colonies in high fidelity and in colour. Differently coloured light sources are turned on and off in sequence and for each illumination colour an image is captured by a monochrome camera. The resultant respective monochrome images are combined into a composite colour image of the microbial colonies.

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31-05-2012 дата публикации

Multi-Shell Microspheres With Integrated Chromatographic And Detection Layers For Use In Array Sensors

Номер: US20120135396A1
Принадлежит: University of Texas System

The development of miniaturized chromatographic systems localized within individual polymer microspheres and their incorporation into a bead-based cross-reactive sensor array platform is described herein. The integrated chromatographic and detection concept is based on the creation of distinct functional layers within the microspheres. In this first example of the new methodology, complexing ligands have been selectively immobilized to create “separation” layers harboring an affinity for various analytes. Information concerning the identities and concentrations of analytes may be drawn from the temporal properties of the beads' optical responses, Varying the nature of the ligand in the separation shell yields a collection of cross-reactive sensing elements well suited for use in array-based micro-total-analysis systems.

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07-06-2012 дата публикации

particle detectors

Номер: US20120140231A1
Принадлежит: Xtrails Tech Ltd

A beam detector ( 10 ) including a light source ( 32 ), a receiver ( 34 ), and a target ( 36 ), acting in co-operation to detect particles in a monitored area ( 38 ). The target ( 36 ), reflects incident light ( 40 ), resulting in reflected light ( 32 ) being returned to receiver ( 34 ). The receiver ( 34 ) is a receiver is capable of recording and reporting light intensity at a plurality of points across its field of view. In the preferred form the detector ( 10 ) emits a first light beam ( 3614 ) in a first wavelength band; a second light beam ( 3618 ) in a second wavelength band; and a third light beam ( 3616 ) in a third wavelength band, wherein the first and second wavelengths bands are substantially equal and are different to the third wavelength band.

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09-08-2012 дата публикации

Minimally invasive cytometry system with qcl inspection of single cells for cancer detection

Номер: US20120202277A1
Принадлежит: 1087 SYSTEMS Inc

This disclosure concerns a minimally invasive cytometry system including a handling system that presents single cells to at least one QCL laser source. The QCL laser source is configured to deliver light to a cell within the cells in order to induce vibrational bond absorption in one or more analytes within the cell. The cytometry system also includes a detection facility that detects the mid-infrared wavelength light transmitted by the cell and identifies the cell as either cancerous or non-cancerous.

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06-09-2012 дата публикации

Device for Determining Particle Sizes

Номер: US20120224166A1
Автор: Martin Heine, Stefan Manz
Принадлежит: BUEHLER AG

Method for measuring particle size distributions, in particular for the optical measurement of wide particle size distributions of bulk materials such as cereals, cereal milling products, cereal products and the like, which is intended to enable the measurement of particle size distributions which vary by orders of magnitude. To address this problem, a sample of isolated particles is optically detected in an arrangement by means of at least two measurement methods, wherein preferably detection of the contours of the particles and laser diffraction take place at the same time.

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08-11-2012 дата публикации

White Blood Cell Analysis System and Method

Номер: US20120282598A1
Автор: Giacomo Vacca, Jiong Wu
Принадлежит: ABBOTT LABORATORIES

Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WB Cs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.

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22-11-2012 дата публикации

Sorting of adherent cells by selective transformation of labels

Номер: US20120295798A1
Принадлежит: Bio Rad Laboratories Inc

Adherent cells bearing characteristics that are detectable only in the adherent state can be sorted on the basis of these characteristics independently of their adherent state, by applying a transformable label to the entire population of cells, both those bearing the characteristics of interest and those not, in their adherent state and identifying the locations of the cells of interest on the adherent surface. The cells of interest, or all cells other than those of interest, are then selectively treated to transform the labels and achieve differentiation between the cells of interest and the remaining cells. All cells are then released from the adherent state and sorted in the same manner as non-adherent cells but on the basis of whether the labels are transformed or not transformed.

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06-12-2012 дата публикации

Particle Analysis in an Acoustic Cytometer

Номер: US20120304749A1
Принадлежит: Los Alamos National Security LLC

The present invention is a method and apparatus for acoustically manipulating one or more particles.

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20-12-2012 дата публикации

Biochemical analysis instrument

Номер: US20120322679A1
Принадлежит: Oxford Nanopore Technologies PLC

An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.

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03-01-2013 дата публикации

Systems and methods for sample display and review

Номер: US20130002847A1
Принадлежит: Constitution Medical Inc

Methods and systems for displaying images of cells in a sample include obtaining a plurality of images of cells in the sample, where each image corresponds to one of the cells in the sample, determining values of at least one property for each of the cells based on the plurality of images, arranging the plurality of images to form a first image array, where the images are ordered in the first image array based on the values of the at least one property, displaying the first image array, sorting the plurality of images to form a second image array in which an ordering of the images is different from the first image array, and displaying the second image array, where the sample includes blood and the cells include red blood cells.

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03-01-2013 дата публикации

System and method for automatic color segmentation and minimum significant response for measurement of fractional localized intensity of cellular compartments

Номер: US20130004053A1
Принадлежит: Vala Sciences Inc

A system, a method, and a programmed device for measurement of translocational activity among cellular compartments process magnified images of cellular material exposed to an agent by segmenting and compartmentalizing the images and then measuring fractional localized intensity of two or more compartments in the segmented, compartmentalized image. The measured fractional localized intensities are compared to determine translocation of cellular material among the measured components caused by the agent.

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14-03-2013 дата публикации

Fine particle measuring apparatus

Номер: US20130065269A1
Автор: Nao Nitta
Принадлежит: Sony Corp

A fine particle measuring apparatus is provided. The fine particle measuring apparatus includes a detection unit configured to detect light emitted from a fine particle and a processing unit having a memory device storing instructions which when executed by the processing unit, cause the processing unit to calculate a corrected intensity value of the detected light and generate spectrum data based on the corrected intensity value.

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21-03-2013 дата публикации

Method and apparatus for measuring optical properties of particles of a dispersion

Номер: US20130070243A1
Автор: Wolfgang Goehde
Принадлежит: Partec GmbH

Disclosed is apparatus for measuring optical properties of particles of a flowable dispersion using a measuring cuvette. The dispersion flows through the central inner chamber of the cuvette. Two laser light beams, which are offset 90 degrees to one another, illuminate the inner chamber of the cuvette, so as to illuminate a particle, regardless of its orientation, in a way that balances out form factor errors.

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27-06-2013 дата публикации

Imaging and evaluating embryos, oocytes, and stem cells

Номер: US20130162795A1
Принадлежит: Leland Stanford Junior University

Methods, compositions and kits for determining the developmental potential of one or more embryos or pluripotent cells and/or the presence of chromosomal abnormalities in one or more embryos or pluripotent cells are provided. These methods, compositions and kits find use in identifying embryos and oocytes in vitro that are most useful in treating infertility in humans.

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04-07-2013 дата публикации

Convex Lens-Induced Confinement for Measuring Distributions of Molecular Size

Номер: US20130170026A1
Принадлежит: Individual

A curved surface is placed tangent to a slide and displaces a sample liquid from the point or line of contact outward. Imaging indicates a region where fluorescence is observed, and the location of the fluorescence indicates the molecular size. The radius of curvature of the lens is known, the distance from the (center) point of contact of the observed fluorescence is measured with a microscope and the distance of the lens surface to the slide's surface can then be calculated. This distance represents the size of the molecule or ensemble of molecules emitting. Similarly, absorbance, etc. could be measured with a light source below the slide.

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18-07-2013 дата публикации

Interactive and automated tissue image analysis with global training database and variable-abstraction processing in cytological specimen classification and laser capture microdissection applications

Номер: US20130182922A1
Автор: David H. Kil
Принадлежит: Life Technologies Corp

A system and method for performing tissue image analysis and region of interest identification for further processing applications such as laser capture microdissection is provided. The invention provides three-stage processing with flexible state transition that allows image recognition to be performed at an appropriate level of abstraction. The three stages include processing at one or more than one of the pixel, subimage and object levels of processing. Also, the invention provides both an interactive mode and a high-throughput batch mode which employs training files generated automatically.

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18-07-2013 дата публикации

Cell processing apparatus, sample preparation apparatus, and cell analyzer

Номер: US20130183747A1
Принадлежит: Sysmex Corp

A cell processing apparatus includes a storage container that contains liquid L including a biological sample; a filter that prevents a first cell C 1 in the biological sample from passing and allows a second cell C 2 having a smaller diameter than that of the first cell C 1 to pass; and a filtration cylinder for separating, in the storage container and via the filter, the liquid L into a first liquid L 1 mainly including the first cell C 1 and a second liquid L 2 mainly including the second cell C 2 . A measurement target cell filtered by the filter among other cells can be easily collected.

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15-08-2013 дата публикации

Flow Cytometry For High Throughput Screening

Номер: US20130210672A1
Принадлежит: STC UNM

The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.

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22-08-2013 дата публикации

Spatially Correlated Light Collection from Multiple Sample Streams Excited with a Line Focused Light Source

Номер: US20130214176A1
Принадлежит: STC UNM

An affordable flow cytometry system with a significantly increased analytical rate, volumetric sample delivery and usable particle size including a light beam that interrogates multiple flow streams so as to provide excitation across all of the streams, and an optical objective configured to collect light from the sample streams and image the light onto an array detector.

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29-08-2013 дата публикации

Efficient haploid cell sorting flow cytometer systems

Номер: US20130224843A1
Принадлежит: XY LLC

A flow cytometry system ( 1 ) for sorting haploid cells, specifically irradiatable sperm cells, with an intermittingly punctuated radiation emitter ( 56 ). Embodiments include a beam manipulator ( 21 ) and even split radiation beams directed to multiple nozzles ( 5 ). Differentiation of sperm characteristics with increased resolution may efficiently allow differentiated sperm cells to be separated higher speeds and even into subpopulations having higher purity.

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05-09-2013 дата публикации

Magnetic particle based biosensor

Номер: US20130230913A1
Автор: Octavian Florescu
Принадлежит: Silicon Biodevices Inc

A biosensor system and method of its use for detecting particles on the surface of an integrated circuit is disclosed. The system can include a light source and a plurality of optical sensors formed on an integrate circuit. The particles can be positioned the surface of the integrated circuit whereby the particles can cast a shadow or shadows that reduces the amount of light transmitted from the light source to the optical sensors. The surface of the integrated circuit can include one or more optical sensing areas whereby the presence of one or more particles may significantly or measurably reduce the amount of light incident on one or more optical sensor.

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26-09-2013 дата публикации

Method for imaging and differential analysis of cells

Номер: US20130251234A1
Принадлежит: Amnis LLC

Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.

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17-10-2013 дата публикации

Device for performing a blood, cell, and/or pathogen count and methods for use thereof

Номер: US20130273524A1
Автор: Joel R. L. Ehrenkranz
Принадлежит: Joel R. L. Ehrenkranz

Devices and methods for performing a point of care blood, cell, and/or pathogen count or a similar blood test. Disclosed herein are systems that can be used to provide rapid, accurate, affordable laboratory-quality testing at the point of care. The systems described herein are capable of imaging and counting individual cells in a prepared cell sample (e.g., a peripheral blood smear or a blood sample prepared in a microfluidic device) or another prepared cell-containing sample without the need for a microscope or other expensive and cumbersome optics. The systems described herein are designed to eliminate or replace expensive, centralized clinical testing equipment and technical personnel. Such systems may include automated data reporting and decision support.

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31-10-2013 дата публикации

Systems and methods for performing quality review scoring of biomarkers and image analysis methods for biological tissue

Номер: US20130287283A1
Принадлежит: General Electric Co

Exemplary embodiments include methods, systems, and devices for enabling users to provide quality scores for indicating the quality of image analysis methods performed on images of biological tissue. An exemplary user interface displays results of an image analysis method performed on an image of biological tissue in an overlaid manner on an image of biological tissue. The exemplary user interface enable a user to provide, directly on the user interface, one or more quality scores to indicate the user's assessment of the quality of the image analysis performed on the image. Exemplary embodiments store the quality scores provided by the user in association with the image analysis method and the image of biological tissue.

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12-12-2013 дата публикации

System and Method for Measuring Particles in a Sample Stream of a Flow Cytometer Using Low-Power Laser Source

Номер: US20130330763A1
Принадлежит: Los Alamos National Security, LLC

A system and method for analyzing a particle in a sample stream of a flow cytometer or the like. The system has a light source, such as a laser pointer module, for generating a low powered light beam and a fluidics apparatus which is configured to transport particles in the sample stream at substantially low velocity through the light beam for interrogation. Detectors, such as photomultiplier tubes, are configured to detect optical signals generated in response to the light beam impinging the particles. Signal conditioning circuitry is connected to each of the detectors to condition each detector output into electronic signals for processing and is designed to have a limited frequency response to filter high frequency noise from the detector output signals. 1. A particle interrogation system comprising:a flow chamber;a fluid container in connection with the flow chamber, the fluid container configured to deliver a gravity fed fluid to the flow chamber;a laser source configured to impinge a laser beam on one or more particles flowing in the flow chamber;a particle delivery system operably coupled to the flow chamber and configured to deliver a sample fluid containing one or more particles in the flow chamber,the particle delivery system being configured to provide a transit time of between at least about 100 microseconds to about 1 millisecond for a particle transported through the laser beam;at least one detector configured to receive one or more optical signals resulting from fluorescence or light scattered from the one or more particles; andsignal conditioning circuitry, operably coupled to the at least one detector.2. The system of claim 1 , wherein the laser source comprises a non-stabilized compact laser.3. The system of claim 1 , wherein the fluid container is connected to the flow chamber via a gravity fed sheath delivery line.4. The system of claim 1 , wherein the signal conditioning circuitry comprises a pre-amplifier stage coupled to the output of the ...

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26-12-2013 дата публикации

METHOD AND FLOW CELL FOR CHARACTERIZING PARTICLES BY MEANS OF NON-GAUSSIAN TEMPORAL SIGNALS

Номер: US20130342837A1
Принадлежит: Handyem inc.

The present disclosure relates to the field characterization of particles in a sample solution. More specifically, the present disclosure relates to a flow cell and a method for characterizing particles by means of collected non-Gaussian temporal signals. The present flow cell and method rely on an excitation fiber with a channel. The excitation fiber has a core for transporting an excitation light generated by a light source, and defines a channel through a portion of its core. The channel of the excitation fiber directs a flow of the sample solution. The excitation fiber, the channel and collection fibers characteristics are selected, proportioned and positioned to generate collected light with a non-Gaussian temporal intensity profile. 1. A flow cell for characterizing particles in a sample solution , the flow cell comprising:an excitation fiber having a core for transporting an excitation light, the excitation fiber defining a channel transversal to its core for directing a flow of the sample solution there through; andat least one collection fiber adjacent to the channel, the at least one collection fiber collecting light scattered or emitted by the particles flowing through the channel and excited by the excitation light;wherein the excitation fiber, the channel and the at least one collection fiber characteristics are selected, proportioned and positioned relative to each other so as to generate collected light of a non-Gaussian temporal intensity profile.2. The flow cell of claim 1 , wherein the excitation fiber claim 1 , the channel and the at least one collection fiber characteristics are selected claim 1 , proportioned and positioned relative to each other so as to generate collected light of a non-Gaussian temporal intensity profile for a range of particle sizes.3. The flow cell of claim 1 , wherein the excitation fiber claim 1 , the channel and the at least one collection fiber characteristics are selected claim 1 , proportioned and positioned relative ...

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26-12-2013 дата публикации

Optical particle detecting device and particle detecting method

Номер: US20130342838A1
Автор: Seiichiro Kinugasa
Принадлежит: Azbil Corp

An optical particle detecting device including a light source that emits an inspection light, a converting unit that converts the inspection light into collimated light, a focusing reflecting mirror that reflects toward a focal point the inspection light that has been converted into collimated light, a jet mechanism that causes an airstream including a particle to jet into the focal point of the focusing reflecting mirror, and a detecting portion that detects either scattered light or fluorescence produced by the particle included in the airstream being illuminated by the inspection light.

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26-12-2013 дата публикации

Nucleic Acid Analysis by Random Mixtures of Non-Overlapping Fragments

Номер: US20130345070A1
Автор: Radoje Drmanac
Принадлежит: Callida Genomics Inc

The invention provides methods and kits for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered. In one aspect, this process is carried out in a hierarchical fashion until the one or more target polynucleotides are characterized, e.g. by their nucleic acid sequences, or by an ordering of sequence segments, or by an ordering of single nucleotide polymorphisms (SNPs), or the like.

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09-01-2014 дата публикации

Nucleic Acid Analysis by Random Mixtures of Non-Overlapping Fragments

Номер: US20140011688A1
Автор: Radoje Drmanac
Принадлежит: Callida Genomics Inc

The invention provides methods and kits for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered. In one aspect, this process is carried out in a hierarchical fashion until the one or more target polynucleotides are characterized, e.g. by their nucleic acid sequences, or by an ordering of sequence segments, or by an ordering of single nucleotide polymorphisms (SNPs), or the like.

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13-02-2014 дата публикации

Method and Apparatus for Tracking a Particle, Particularly a Single Molecule, in a Sample

Номер: US20140042340A1
Автор: Stefan W. Hell

For the purpose of tracking a movement of a particle in a sample, the particle is driven by light to emit photons, and the photons emitted by the particle are detected. The light applied to the sample features a light intensity distribution with a spatially limited minimum. The particle is tracked with the minimum of the light intensity distribution by moving the light intensity distribution with respect to the sample such that a rate of photons emitted by the particle remains minimal, and by taking an actual position of the minimum of the light intensity distribution in the sample as an actual position of the particle in the sample.

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20-03-2014 дата публикации

Nozzle assembly for a flow cytometer system and methods of manufacture

Номер: US20140076986A1
Принадлежит: INGURAN LLC

A method of manufacturing a nozzle assembly may include the step of over molding a nozzle housing, or a portion of a nozzle housing, onto at least one nozzle component, such as an injection tube. Nozzle assemblies and flow cytometers incorporating nozzle assemblies may include any combination of straight smooth injection tubes, improved features for securing a nozzle assembly, improved features for debubbling a nozzle assembly, and aggressive orienting geometries. A method of sorting cells may include the step of magnetically coupling a nozzle assembly with a flow cytometer.

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27-03-2014 дата публикации

MICROPARTICLE ANALYSIS APPARATUS AND MICROPARTICLE ANALYSIS METHOD

Номер: US20140087453A1
Автор: Tahara Katsutoshi
Принадлежит: SONY CORPORATION

There is provided a microparticle analysis apparatus including a light detection unit configured to detect forward-scattered light generated from a microparticle that is an analysis target. The light detection unit includes a circuit having a high-pass filter that removes low frequency noise included in light entering the light detection unit and switches to the high-pass filter according to a predetermined frequency of the forward-scattered light. 1. A microparticle analysis apparatus comprising:a light detection unit configured to detect forward-scattered light generated from a microparticle that is an analysis target,wherein the light detection unit includes a circuit having a high-pass filter that removes low frequency noise included in light entering the light detection unit and switches to the high-pass filter according to a predetermined frequency of the forward-scattered light.2. The microparticle analysis apparatus according to claim 1 , wherein the light detection unit switches to the high-pass filter when the predetermined frequency is equal to or higher than 200 kHz.3. The microparticle analysis apparatus according to claim 2 , wherein the high-pass filter removes low frequency noise included in leakage light which avoids a zero-order light removal unit arranged between the microparticle and the light detection unit and which enters into the light detection unit.4. The microparticle analysis apparatus according to claim 3 , wherein the high-pass filter removes noise of a frequency lower than 2 kHz.5. The microparticle analysis apparatus according to claim 4 ,wherein the circuit includes a channel directly connected from an input side to an output side and a channel having the high-pass filter, andwherein the channel directly connected and the channel having the high-pass filter are connected in parallel.6. The microparticle analysis apparatus according to claim 5 , wherein the circuit includes a switch element to perform switching to one of the channel ...

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04-01-2018 дата публикации

DEVICES AND METHODS FOR FRACTIONATED PHOTOACOUSTIC FLOW CYTOMETRY

Номер: US20180000351A1
Принадлежит:

A fractionated photoacoustic flow cytometry (PAFC) system and methods for the in vivo detection of target objects in biofluidic systems (e.g., blood, lymph, urine, or cerebrospinal fluid) of a living organism is described. The fractionated system includes a fractionated laser system, a fractionated optical system, a fractionated acoustic system, and combinations thereof. The fractionated laser system includes at least one laser or laser array for pulsing a target object within the circulatory vessel with fractionated focused laser beams. The fractionated optical system separates one or several laser beams into multiple beams in a spatial configuration on the skin above the circulatory vessel of the living organism. The fractionated acoustic system includes multiple focused ultrasound transducers for receiving photoacoustic signals emitted by the target object in response to the fractionated laser beams. The target objects have intrinsic photoacoustic contrast or may be labeled with photoswitchable or spaser-based probes. Fractioned beams may be used also for diagnostics with other spectroscopic methods (e.g., fluorescence, Raman or scattering) and energy sources both coherent and conventional such as lamp and LED in the broad spectral range from 10 Å to 1 cm (e.g., X-ray, UV, visible, NIR or microwaves) in continuous wave and pulse modes. 1. A fractionated photoacoustic flow cytometry system for the in vivo detection of target objects in a biofluid system of a living organism , comprising:a laser system comprising at least one laser comprising at least one wavelength for providing at least one laser beam to at least one target object within the biofluid system;a fractionated optical system configured to separate the at least one laser beam into fractionated laser beams having a spatial configuration on the skin above the biofluid system of the living organism; andan acoustic system comprising at least one focused ultrasound transducer for receiving more than one ...

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06-01-2022 дата публикации

Systems And User Interface For Collecting A Data Set In A Flow Cytometer

Номер: US20220003658A1
Автор: Rich Collin A.
Принадлежит:

Systems in a flow cytometer having an interrogation zone and illumination impinging the interrogation zone include: a lens subsystem including a collimating element that collimates light from the interrogation zone, a light dispersion element that disperses collimated light into a light spectrum, and a focusing lens that focuses the light spectrum onto an array of adjacent detection points; a detector array, including semiconductor detector devices, that collectively detects a full spectral range of input light signals, in which each detector device detects a subset spectral range of the full spectral range of light signals; and a user interface that enables a user to create a set of virtual detector channels by grouping detectors in the detector array, such that each virtual detector channel corresponds to a detector group and has a virtual detector channel range including the sum of subset spectral ranges of the detectors in the corresponding detector group. 19-. (canceled)10. A light detection system comprising:a plurality of user-configurable groups of photodetectors; anda plurality of detector channels, wherein each detector channel is assigned to one or more of the user-configurable groups of photodetectors.11. The light detection system according to claim 10 , wherein the light detection system is configured to:detect light from a sample irradiated with a light source; andgenerate a data signal from the detected light.12. The light detection system according to claim 11 , wherein the light detection system is operably coupled to a processor comprising memory having instructions stored thereon claim 11 , which when executed by the processor claim 11 , cause the processor to assign each detector channel to a user-configurable group of photodetectors before generating a data signal from the light detected from the irradiated sample.13. The light detection system according to claim 11 , wherein the light detection system is operably coupled to a processor ...

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06-01-2022 дата публикации

METHOD AND SYSTEM FOR CHARACTERIZING PARTICLES USING AN ANGULAR DETECTION IN A FLOW CYTOMETER

Номер: US20220003660A1
Принадлежит:

The invention relates to a method and system for characterizing particles using a flow cytometer comprising detecting radiated light from the particles using two or more detectors positioned to allow for the detection in two or more angular directions and generating a waveform, as a digital representation for the detected radiated light for each of said angulation direction. The waveforms are transformed using one or more basis functions to obtain one or more coefficients characterizing the waveform. The one or more coefficients characterizing the waveform preferably correspond to properties of the particle(s), thereby enabling analysis of physical properties of the particles (such as size, shape, refractive index) or biological properties of the particles (such as cell type, cell cycle state or localization or distribution of molecules within the cell and/or on the cell surface). In preferred embodiments the method and system are used for a label-free sorting of particles, in particular biological cells. 1. A method for characterizing particles using a flow cytometer comprising:a. passing of one or more particles in a fluid stream through a light beam of the flow cytometer,{'b': '3', 'b. detecting radiated light as one or more particles pass through the light beam using two or more detectors positioned to allow for the detection of the radiated light () in two or more angular directions,'}c. generating for each of the angular directions a waveform which is a digital representation of the detected radiated light for said angular direction, andd. transforming each waveform using one or more basis functions and obtaining one or more coefficients characterizing the waveform.2. Method according to claim 1 , wherein the detected radiated light is a forward scatter signal claim 1 , a side scatter signal and/or a fluorescence light and the two or more detectors are positioned to allow for detection of a forward scatter signal claim 1 , a side scatter signal and/or a ...

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07-01-2016 дата публикации

Filter and Blower Geometry for Particle Sampler

Номер: US20160002700A1
Принадлежит: PARTICLE MEASURING SYSTEMS, INC.

The invention provides devices and methods for sampling, detecting and/or characterizing particles. Devices and methods of the invention, including particle samplers, impactors and counters, include a filter component for removing particles in the exhaust flow of the device, for example, to eliminate or minimize the potential for the device itself to provide source of particles in an environment undergoing particle monitoring. This aspect of the present devices and methods is particularly useful for monitoring particles in manufacturing environments requiring low levels of particles, such as cleanroom environments for electronics manufacturing and aseptic environments for manufacturing pharmaceutical and biological products. 1. A sampler comprising:one or more fluid inlets for sampling a fluid flow;a particle analysis or collection region positioned in fluid communication with said one or more fluid inlets;a fan or pump positioned in fluid communication with said particle analysis or collection region, said fan or pump for generating said fluid flow, wherein said fan or pump comprises a motor; anda filter in fluid communication with said fan or pump and positioned around at least a portion of said motor, said filter for filtering said fluid flow exhausted from said fan or pump.2. (canceled)3. The sampler of claim 1 , wherein said filter has a toroid shape and said motor is positioned in a vacant central region of said toroid shape.4. The sampler of claim 1 , wherein said filter has a cylindrical shape and said motor is positioned in a central aperture of said cylindrical shape.5. The sampler of claim 4 , wherein said fan has a rotational axis and wherein said cylindrical shape has a cylindrical axis and wherein said rotational axis and said cylindrical axis are parallel.6. (canceled)7. The sampler of claim 1 , wherein said fluid flows through said one or more fluid inlets claim 1 , through said particle analysis or collection region claim 1 , into an intake of said ...

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05-01-2017 дата публикации

Analysis Device and Analysis Method

Номер: US20170003213A1
Принадлежит:

An analysis device optically scans a surface of a substrate to which analytes and particles for labeling the analytes are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the analytes and determines that the analyte count is one when two pulse waves are detected consecutively each having pulse width less than first reference value determined depending on first pulse width in the detection signal when the optical scanning unit scans a plurality of particles adjacent to each other. 1. An analysis device comprising:an optical scanning unit configured to optically scan a surface of a substrate to which analytes and particles for labeling the analytes are fixed;a pulse detector configured to detect a pulse wave and a pulse width of the pulse wave included in a detection signal obtained from the optical scanning unit when the optical scanning unit scans the substrate; anda counting unit configured to count the analytes and determine that an analyte count is one when the pulse detector consecutively detects two pulse waves each having a pulse width less than a first reference value.2. The analysis device according to claim 1 , wherein the counting unit determines that the analyte count is one when the pulse detector detects a pulse wave having a pulse width greater than or equal to the first reference value and less than a second reference value.3. The analysis device according to claim 1 , wherein claim 1 , when the pulse detector detects a first pulse wave having a pulse width less than the first reference value claim 1 , detects a second pulse wave after the first pulse wave claim 1 , and a pulse interval between the first pulse wave and the second pulse wave is greater than or equal to a third reference value claim 1 , the counting unit does not implement counting processing with regard to both the first pulse wave and the second pulse wave.4. The analysis ...

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05-01-2017 дата публикации

SPECIAL PURPOSE CUVETTE ASSEMBLY AND METHOD FOR OPTICAL MICROSCOPY OF NANOPARTICLES IN LIQUIDS

Номер: US20170003214A1
Принадлежит: MANTA Instruments, Inc.

A special purpose cuvette assembly with features that create a small, restricted volume to minimize bulk movements of liquid and that minimize backscattering of light. The special-purpose cuvette assembly enables recording of Brownian movements of nanoparticles in a liquid when it is placed in a suitable optical device comprising a light sheet and an optical microscope attached to a video camera that is oriented perpendicular to the light-sheet plane. 1. A system for viewing particles , the system comprising:a light source for generating an electromagnetic energy directed at a cuvette;a sensor external to said cuvette for detecting electromagnetic energy within the cuvette; and 'exterior walls and a floor that define a volume, wherein at least a portion of the exterior walls is transparent to the electromagnetic energy, and wherein the volume is adapted to contain a suspension liquid and the particles;', 'the cuvette comprising an upper viewing chamber wall extending from the exterior walls and a lower viewing chamber wall extending from the exterior walls, wherein the upper and lower viewing chamber walls are substantially parallel to the floor;', 'a reflecting wall adjacent to the upper and lower viewing chamber walls;, 'a viewing chamber comprisinga backscatter chamber separated from and in fluid communication with the viewing chamber, wherein the reflecting wall is adapted to reflect the electromagnetic energy into the backscatter chamber.2. The system of claim 1 , wherein the cuvette further comprises:a mixing chamber separated from and in fluid communication with the viewing chamber, the mixing chamber including a mixing stick.3. The system of claim 1 , wherein the reflecting wall forms an angle with the lower viewing chamber wall claim 1 , wherein the angle is between 30 and 60 degrees.4. The system of claim 1 , wherein the reflecting wall comprises a reflective surface.5. The system of claim 1 , wherein the upper and lower viewing chamber walls have a very- ...

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05-01-2017 дата публикации

DYNAMIC LIGHT SCATTERING BASED MICRORHEOLOGY OF COMPLEX FLUIDS WITH IMPROVED SINGLE-SCATTERING MODE DETECTION

Номер: US20170003220A1
Принадлежит:

A fluid characterization measuring instrument is disclosed that comprises a sample vessel for a bulk complex sample fluid having a capacity that is substantially larger than a domain size of the complex sample fluid and that is sufficiently large to cause bulk scattering effects to substantially exceed surface effects for the complex fluid sample, a coherent light source positioned to illuminate the bulk complex sample fluid in the sample vessel and a first fibre having a first end positioned to receive backscattered light from the sample after it has interacted with the sample. The first fibre can also be positioned close enough to an optical axis of the coherent light source and to the sample vessel to substantially decrease a contribution of multiply scattered light in the backscattered light. The instrument can further comprise a first photon-counting detector positioned to receive the backscattered light from a second end of the fibre, correlation logic responsive to the first photon-counting detector and single-scattering fluid property analysis logic responsive to the correlation logic and operative to derive at least one fluid property for the sample fluid. 115-. (canceled)16. A fluid characterization measuring instrument , comprising:a capillary tube for a sample fluid,a plurality of probe particles for the sample fluid,a coherent light source positioned to illuminate the sample fluid and probe particles in the capillary tube,a photon-counting detector positioned to receive scattered light from the capillary tube, andfluid property analysis logic responsive to the detector and operative to derive at least one fluid property for the sample fluid.17. The instrument of further including an optical fiber between the capillary tube and the photon-counting detector.18. The instrument of wherein the optical fiber has a first end positioned to receive backscattered light from the sample after it has interacted with the sample.19. The instrument of wherein the ...

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05-01-2017 дата публикации

PARTICLE MEASURING DEVICE

Номер: US20170003221A1
Принадлежит: FUJI ELECTRIC CO., LTD.

A particle measuring device includes: an optical resonator that reflects laser light back and forth between two facing reflective mirrors in order to amplify an energy of that laser light and form resonant laser light; a particle transport unit that transports particles in an aerosol to be measured across a beam path of the resonant laser light; a scattered light receiving unit that receives scattered light produced when the particles in the aerosol are irradiated by the resonant laser light; and a processor that receives light reception signals from the scattered light receiving unit, wherein the processor outputs light reception pulses according to the light reception signals and calculates time intervals between the light reception pulses that are temporally adjacent. 1. A particle measuring device for measuring particles in an aerosol , comprising:an optical resonator that causes laser light to travel back and forth between two opposing reflective mirrors in order to amplify an energy of the laser light and form resonant laser light;a particle transport unit configured to transport the particles in the aerosol across a beam path of the resonant laser light so as to generate a stream of the particles crossing the beam path;a scattered light receiving unit configured to receive scattered light that is produced when the particles in the aerosol are irradiated by the resonant laser light, and output a light reception signal in accordance with the received scattered light for each scattering event; anda processor that receives the light reception signal from the scattered light receiving unit for each event of the reception of the scattered light,wherein the processor outputs a light reception pulse in accordance with each light reception signal from the scattered light receiving unit, and derives time intervals between light reception pulses that are temporally adjacent.2. The particle measuring device according to claim 1 , wherein the processor further derives a ...

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05-01-2017 дата публикации

SHEATH FLUID SYSTEMS AND METHODS FOR PARTICLE ANALYSIS IN BLOOD SAMPLES

Номер: US20170003273A1
Принадлежит:

Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. 1. A particle and intracellular organelle alignment liquid (PIOAL) for use in a combined viscosity and geometric hydrofocusing analyzer , the PIOAL directing flow of a blood sample fluid of a given viscosity that is injected into a narrowing flowcell transition zone of the visual analyzer so as to produce a sample fluid stream enveloped by the PIOAL , the PIOAL comprising:a fluid having a higher viscosity than the viscosity of the blood sample fluid,a pH adjusting agent, andProcaine HCl,wherein a viscosity hydrofocusing effect induced by an interaction between the PIOAL fluid and the sample fluid associated with the viscosity difference, in combination with a geometric hydrofocusing effect induced by an interaction between the PIOAL fluid and the sample fluid associated with the narrowing flowcell transition zone, is effective to provide a target imaging state in at least some of the plurality of particles at an imaging site of the visual analyzer while a viscosity agent in the PIOAL retains viability of cells in the sample fluid stream leaving structure and content of the cells intact when the cells extend from the sample fluid stream into the flowing sheath fluid, andwherein the viscosity agent of the sheath fluid comprises glycerol at a concentration between about 1 to about 50% (v/v).2. The PIOAL of claim 1 , wherein the viscosity agent of the sheath fluid comprises polyvinylpyrrolidone (PVP).3. The PIOAL of claim 2 , wherein the polyvinylpyrrolidone (PVP) is at a concentration of 1% (w/v).4. The PIOAL of claim 1 , wherein the viscosity agent of the sheath fluid comprises glycerol at a concentration of 5% (v/v) and polyvinylpyrrolidone (PVP) ...

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07-01-2016 дата публикации

PHOTODETECTION DEVICE

Номер: US20160003727A1
Автор: Tanaka Masaki
Принадлежит: SHARP KABUSHIKI KAISHA

A photodetection device has an optical module () that includes a light source (), an excitation optical system, and a detection optical system and that two-dimensionally and relatively scans a transparent stage () in a first sampling direction and a second sampling direction intersecting the first sampling direction. A scan length in the first sampling direction is longer than a scan length in the second sampling direction. A data sampling unit in the detection optical system performs sampling for a distance of a second sampling interval during scanning in the second sampling direction, and performs sampling for a distance of a first sampling interval shorter than the distance of the second sampling interval during scanning in the first sampling direction. An aperture () of the excitation optical system sets a size in the first sampling direction of a spot shape of excitation light from the light source () to be smaller than a size in the second sampling direction. 1. A photodetection device comprising:a light-transmitting transparent stage on which a detection object is placed;an excitation optical system that irradiates the detection object with excitation light emitted from a light source;a detection optical system that detects light emitted from a detection surface of the detection object placed on the transparent stage by irradiation with the excitation light;a data sampling unit included in the detection optical system to sample an intensity of the detected light at a predetermined set interval; andan optical module that includes the light source, the excitation optical system, and the detection optical system and that two-dimensionally and relatively scans the transparent stage in a first sampling direction of the data sampling unit and a second sampling direction intersecting the first sampling direction,wherein a scan length in the first sampling direction in the optical module is longer than a scan length in the second sampling direction,wherein the data ...

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07-01-2016 дата публикации

METHOD OF AND APPARATUS FOR ASCERTAINING THE SIZE OF PARTICLES

Номер: US20160003728A1
Автор: Hardalupas Yannis
Принадлежит:

A method of ascertaining the size of small particles is disclosed. The method includes the steps of: a) intersecting at least two light beams at an intersection volume; b) sensing at each of a plurality of sensing positions angularly displaced from one another light scattered by a particle substantially in the intersection volume, and producing respective output signals indicative of the sensed light; c) ascertaining the phase difference between one of the signals and each other of the signals to give a measured indication of the variation of phase difference with angular displacement; and d) comparing the measured indication with at least one known indication of the variation of phase difference with angle for a known particle size and thereby determining the size of the particle substantially in the intersection volume. 1. A method of ascertaining the size of small particles , the method including the steps of:a) intersecting at least two light beams at an intersection volume;b) sensing at each of a plurality of sensing positions angularly displaced from one another light scattered by a particle substantially in the intersection volume, and producing respective output signals indicative of the sensed light;c) ascertaining the phase difference between one of the signals and each other of the signals to give a measured indication of the variation of phase difference with angular displacement; andd) comparing the measured indication with at least one known indication of the variation of phase difference with angle for a known particle size and thereby determining the size of the particle substantially in the intersection volume.2. A method according to claim 1 , wherein the measured indication of the variation of phase difference with angular displacement includes an indication of the angular position of transitions between local maxima and minima of the phase difference.3. A method according to claim 2 , wherein the measured indication includes information ...

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07-01-2016 дата публикации

FLUIDIC FLOW CYTOMETRY DEVICES AND PARTICLE SENSING BASED ON SIGNAL-ENCODING

Номер: US20160003729A1
Принадлежит:

Microfluidic devices, systems and techniques in connection with particle sorting in liquid, including cytometry devices and techniques and applications in chemical or biological testing and diagnostic measurements. 1. A particle sorter for sorting particles in a fluid , comprising:a structure having an input channel connected at an actuation area to a plurality of output channels, wherein the particles in the fluid flow through the input channel to the actuation area, and each particle travels from the actuation area to one of the plurality of output channels, anda piezoelectric actuator for causing a flow disturbance in the actuation area in response to a control signal, wherein the flow disturbance operates to direct a particle along a trajectory to one of the plurality of output channels which is different than the output channel to which the particle would travel without the flow disturbance.2. The particle sorter of claim 1 , wherein the structure includes at least one of a polymer substrate claim 1 , a polydimethylsiloxine (PDMS) substrate claim 1 , or a glass substrate.3. The particle sorter of claim 2 , wherein the piezoelectric actuator is permanently bonded via UV ozone treatment to the PDMS substrate.4. The particle sorter of claim 1 , wherein the piezoelectric actuator is integrated with the structure.5. The particle sorter of claim 1 , further comprising a driver for generating the control signal.6. The particle sorter of claim 1 , wherein the control signal is a voltage signal having a controlled magnitude and frequency.7. The particle sorter of claim 1 , wherein the detection unit comprises a bank of filters for detecting a signal from the particle.8. The particle sorter of claim 1 , wherein the piezoelectric actuator includes a contact layer for coupling the piezoelectric actuator to the structure claim 1 , and further includes a piezoelectric layer for generating a signal to cause the flow disturbance in response to the control signal.9. The ...

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07-01-2016 дата публикации

FLOW CYTOMETRY SYSTEM AND METHOD

Номер: US20160003730A1
Принадлежит:

A flow cytometry system having a flow channel defined through the thickness of a substrate is disclosed. Fluid flowing through the flow channel is illuminated by a first plurality of surface waveguides that are arranged around the flow channel in a first plane, while a second plurality of surface waveguides arranged around the flow channel in a second plane receive light after it has interacted with the fluid. The illumination pattern provided to the fluid is controlled by controlling the phase of the light in the first plurality of surface waveguides. As a result, the fluid is illuminated with light that is uniform and has a low coefficient of variation, improving the ability to distinguish and quantify characteristics of the fluid, such as cell count, DNA content, and the like. 1. An apparatus comprising:a substrate that defines a first plane, the substrate comprising a flow channel that is operative for conveying fluid along a first direction that is substantially orthogonal to the first plane, the flow channel being located within a first region of the substrate;a first surface waveguide that is optically coupled with the flow channel, the first surface waveguide being located in a second plane within the first region, wherein the second plane is substantially parallel with the first plane; anda second surface waveguide that is optically coupled with the flow channel in the first region, the second surface waveguide being located in a third plane within the first region, wherein the third plane is substantially parallel with the second plane.2. The apparatus of claim 1 , wherein the second plane and the third plane are the same plane.3. The apparatus of claim 1 , further comprising:a first plurality of surface waveguides that includes the first surface waveguide, each of the first plurality of surface waveguides being located in the second plane in the first region and being optically coupled with the flow channel; anda second plurality of surface waveguides ...

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03-01-2019 дата публикации

SYSTEM AND METHOD FOR DROPLET DETECTION

Номер: US20190002956A1
Принадлежит:

Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel. 1. A method of droplet detection , the method comprising:generating a single-file stream of droplets in carrier liquid;combining at least one stream of spacing fluid with the single-file stream of droplets in carrier liquid;directing the combined streams to a detection channel using a spacing channel that tapers toward the detection channel, wherein a distance between adjacent droplets is increased as such droplets travel along the spacing channel toward the detection channel; anddetecting a signal from droplets passing through the detection channel.2. The method of claim 1 , wherein the step of generating includes a step of passing droplets through an alignment region of a sample inlet channel claim 1 , wherein a taper of the alignment region arranges droplets in single file before reaching a channel junction claim 1 , and wherein the step of combining includes a step of combining at least one stream of spacing fluid with the single-file stream of droplets in carrier liquid at the channel junction.3. The method of claim 2 , wherein the step ...

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07-01-2016 дата публикации

Thin film transistor detection systems and related methods

Номер: US20160003953A1
Автор: David Allee, George Kunnen
Принадлежит: Arizona Board of Regents of ASU

Some embodiments include a system. The system includes a sensor device having a sensor element having a sensor output and an amplification element having at least one amplification stage, an amplifier input, and an amplifier output. The sensor output can be coupled to the amplifier input. Further, each amplification stage of the amplification stage(s) can have at least four thin film transistors, an input node, and an output node. Meanwhile, the sensor element can detect a physical quantity and/or an event and can provide an electric signal to the amplification element in response to detecting the physical quantity and/or the event, and the amplification element can amplify the electric signal received from the sensor element. Other embodiments of related systems and methods are also disclosed.

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04-01-2018 дата публикации

Particulate matter detector

Номер: US20180003612A1
Принадлежит: Honeywell International Inc

Devices and methods for detecting particulate matter are described herein. One device includes a laser, a reflector, an ellipsoidal reflector, and a detector, wherein the laser is configured to emit a beam, the reflector is configured to reflect the beam toward the ellipsoidal reflector, and the ellipsoidal reflector has a first focal region located on a path of the reflected beam, and a second focal region located at a surface of the detector.

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02-01-2020 дата публикации

INTEGRATED PRE-AMPLIFICATION LIGHT DETECTION SYSTEMS AND METHODS OF USE THEREOF

Номер: US20200003615A1
Автор: Wu Austin
Принадлежит:

Systems for detecting light (e.g., in a flow stream) are described. Light detection systems according to embodiments include a photodetector, an input modulator configured to modulate signal input into the photodetector and an output modulator configured to modulate signal output from the photodetector. Photodetector arrays having a plurality of light detection systems, e.g., as described, are also provided. Methods for matching output signals from two or more photodetectors (e.g., a plurality of photomultiplier tubes in a photodetector array) are also described. Flow cytometer systems and methods for detecting light from a sample in a flow stream are provided. Aspects further include kits having two or more of the subject light detection systems. 1. A light detection system comprising:a photodetector;an input modulator configured to modulate signal input into the photodetector; andan output modulator configured to modulate signal output from the photodetector.2. The light detection system according to claim 1 , wherein the photodetector comprises a photomultiplier tube.3. The light detection system according to claim 1 , wherein the photodetector comprises a photodiode.4. The light detection system according to claim 3 , wherein the photodiode is an avalanche photodiode.5. The light detection system according to claim 1 , wherein the photodetector comprises a photocathode and an avalanche photodiode.6. The light detection system according to claim 1 , wherein the input modulator comprises an amplifier.7. The light detection system according to claim 6 , wherein the amplifier is a transimpedance amplifier.8. The light detection system according to claim 1 , wherein the input modulator comprises an array of resistors.9. The light detection system according to claim 1 , wherein the input modulator comprises an array of resistors and capacitors.10. The light detection system according to claim 1 , wherein the input modulator is configured to increase the current of the ...

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02-01-2020 дата публикации

LASER SENSOR MODULE FOR PARTICLE DETECTION WITH OFFSET BEAM

Номер: US20200003673A1
Принадлежит:

A laser sensor module for detecting a particle density of particles, which includes: a laser; a detector; and a mirror. The laser is arranged to emit a laser beam to the mirror. A movement of the mirror is arranged to redirect the laser beam. The laser beam is displaced with respect to a rotation axis of the mirror such that a focus region of the laser beam is moving with a velocity having components normal and parallel to the optical axis of the redirected laser beam such that an angle between the parallel and the normal velocity component is at least a threshold angle of 2°. The detector is arranged to determine a self mixing interference signal of an optical wave within a laser cavity of the laser, the self mixing interference signal being generated by laser light of the laser beam reflected by at least one of the particles. 1. A laser sensor module for detecting a particle density of particles with a size of less than 20 μm , wherein the laser sensor module comprises:a laser;a detector; anda mirror rotatable about a rotation axis,wherein the laser beam is focused to a focus region,wherein the laser is arranged to emit a laser beam to the mirror,wherein a movement of the mirror is arranged to dynamically redirect the laser beam,wherein a direction of the redirected laser beam defines an optical axis,wherein the laser beam is displaced with respect to the rotation axis of the mirror such that the focus region of the laser beam is moving with a velocity comprising components normal and parallel to the optical axis of the redirected laser beam such that an angle α between the parallel velocity component with the normal velocity component is at least a threshold angle of 2°, andwherein the detector is arranged to determine a self mixing interference signal of an optical wave within a laser cavity of the laser, the self mixing interference signal being generated by laser light of the laser beam reflected by at least one of the particles.2. The laser sensor module ...

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07-01-2021 дата публикации

Fine particle measurement device

Номер: US20210003494A1
Принадлежит: Sumitomo Electric Industries Ltd

A fine particle measurement device includes a support stand (20) that has a groove (F) extending in a predetermined direction and is configured to support in the groove an observation container (10), which has an elongate shape and accommodates a liquid sample containing a fine particle therein such that an extending direction of the groove (F) coincides with a longitudinal direction of the observation container (10); and an imaging unit (40) that is configured to capture an image of the fine particle in the observation container (10) at a position where the support stand is out of a field of view, the observation container (10) being supported by the support stand (20).

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03-01-2019 дата публикации

SPECTRAL MICROSCOPE

Номер: US20190003892A1

In one implementation, a spectral microscope may comprise a substrate with a planar lens, the planar lens including a phase profile including an axial focus and an oblique focus, a light source to excite a signal of a particle among a plurality of particles, and a detector to receive light generated from the light source from the axial focus of the planar lens and a spectral color component of the excited signal of the particle from the oblique focus of the planar lens. 1. A spectral microscope , comprising:a substrate with a planar lens, the planar lens including a phase profile including an axial focus and an oblique focus;a light source to excite a signal of a particle among a plurality of particles; and light generated from the light source from the axial focus of the planar lens; and', 'a spectral color component of the excited signal of the particle from the oblique focus of the planar lens., 'a detector to receive2. The spectral microscope of claim 1 , wherein the plurality of particles are located in a fluid.3. The spectral microscope of claim 1 , wherein the spectral color component of the oblique focus corresponds to a wavelength of the excited signal.4. The spectral microscope of claim 1 , wherein the phase profile of the planar lens includes:an axial phase profile based on the axial focus of the planar lens; andan oblique phase profile based on the oblique focus of the planar lens.5. The spectral microscope of claim 1 , wherein the planar lens is a diffractive lens.6. The spectral microscope of claim 1 , wherein the light source is a light emitting diode.7. The spectral microscope of claim 1 , wherein the light source is a laser.8. A method claim 1 , comprising:generating, by a light source, an excitation light to excite a plurality of fluorescent signals of a plurality of particles of a fluid, wherein the fluid is located in a channel in a transparent chip;receiving, at a detector from an axial focus of a planar lens, the excitation light;receiving, at ...

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13-01-2022 дата публикации

MICROBIAL CYTOMETRIC MOCK COMMUNITIES AND USE THEREOF AS STANDARD IN FLOW CYTOMETRY

Номер: US20220010351A1
Принадлежит:

The present invention is directed to a microbial Cytometric Mock Community for use in flow cytometric analysis, the microbial Cytometric Mock Community comprising or consisting of cells of at least three different microbial species in a pre-defined ratio, wherein the at least three different microbial species are selected such that, when measured using flow cytometry, the specific gate pattern of each microbial species differs significantly from the specific gate pattern of the other microbial species of the microbial Cytometric Mock Community, preferably the at least three different microbial species differ in relative DNA content, relative genomic GC-content, relative cell size, Gram +/− affiliation and/or capacity to form spores. The microbial Cytometric Mock Community shall serve as standardization means that will help ecologists, microbiologists, molecular biologists and flow cytometrists to work on a standardized basis to allow comparison and exchange of data. 1. Microbial Cytometric Mock Community for use in flow cytometric analysis , the microbial Cytometric Mock Community comprising or consisting of cells of at least three different microbial species in a pre-defined ratio , wherein the at least three different microbial species are selected such that , when measured using flow cytometry , the specific gate pattern of each microbial species differs significantly from the specific gate pattern of the other microbial species of the microbial Cytometric Mock Community , preferably the at least three different microbial species differ in overall DNA content , relative genomic GC-content , average cell size , Gram +/− affiliation and/or capacity to form spores.2. Microbial Cytometric Mock Community of claim 1 , wherein the at least three different microbial species comprise or consist of species derived from archaea claim 1 , bacteria claim 1 , fungi claim 1 , protozoa and algae claim 1 , preferably derived from bacterial species.3. Microbial Cytometric Mock ...

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03-01-2019 дата публикации

Interferometric scattering microscopy

Номер: US20190004299A1
Принадлежит: Oxford University Innovation Ltd

An interferometric scattering microscope is adapted by performing spatial filtering of output light, which comprises both light scattered from a sample location and illuminating light reflected from the sample location, prior to detection of the output light. The spatial filtering passes the reflected illumination light but with a reduction in intensity that is greater within a predetermined numerical aperture than at larger numerical apertures. This enhances the imaging contrast for coherent illumination, particularly for objects that are weak scatterers.

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20-01-2022 дата публикации

METHOD FOR CHARACTERISING A PARTICLE ON THE BASIS OF A HOLOGRAM

Номер: US20220018756A1
Принадлежит:

A method for characterizing a particle present in a sample, the sample lying between an image sensor and a light source and the sensor lying in a detection plane, includes illuminating the sample with the light source which emits an incident light wave propagating along a propagation axis, and acquiring an image of the sample with the sensor. The sensor is exposed to an exposure light wave. The image includes a plurality of elementary diffraction patterns each corresponding to one particle. The method also includes reconstructing a complex image representative of a complex amplitude of the light wave on a reconstruction surface passing through the sample, based on the acquired image; selecting a region of interest of the complex image corresponding to a particle of interest; forming an extracted image based on the region of interest; and characterizing the particle of interest depending on the extracted region of interest. 117-. (canceled)18. A method for characterizing a particle within a sample , the sample lying between an image sensor and a light source , the image sensor lying in a detection plane , the method comprising:a) illuminating the sample with the light source, the light source emitting an incident light wave that propagates along a propagation axis;b) acquiring an image of the sample with the image sensor, the image comprising a plurality of elementary diffraction patterns, each elementary diffraction pattern corresponding to one particle;c) on the basis of the acquired image, reconstructing a complex image representative of a complex amplitude of the exposure light wave on at least one reconstruction surface passing through the sample, the reconstruction being achieved by implementing an iterative reconstruction algorithm, the algorithm comprising, in each iteration, updating a phase of the exposure light wave in the detection plane or on the reconstruction surface;d) selecting a region of interest of the complex image, the selected region of ...

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14-01-2021 дата публикации

Cell capture in microfluidic devices

Номер: US20210008554A1
Принадлежит: Astrego Diagnostics AB

A capturing of target cells from a biological sample is achieved by inducing a flow of the biological sample in a flow channel (30, 60) of an upstream microfluidic device (1). Target cells present in the biological sample are captured in cell channels (20) of the upstream microfluidic device(1). Once at least a minimum number of target cells are captured in the cell channels (20), the flow of the biological sample in the flow channel is reduced and are verse flow is applied at the upstream microfluidic device (1) to release the target cells captured in the cell channels (20) of the upstream microfluidic device (1) and enable transfer the target cells into cell channels (120) of a downstream microfluidic device (100).

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27-01-2022 дата публикации

Optical Measurement System for Real-Time Process Monitoring of Aerosol Jet Printing

Номер: US20220024206A1
Принадлежит:

Aerosol jet printing is a popular digital fabrication method for flexible and hybrid electronics, but it lacks sophisticated process control architectures that would enable more widespread adoption in manufacturing environments. An optical measurement system can be used to track the aerosol density upstream of the printhead. For example, the measured optical extinction combined with the aerosol flow rate, is directly related to deposition rate and accurately predicts functional properties, for example electrical resistance. This real-time system offers a compelling solution for process drift and batch-to-batch variability, a valuable tool for more fundamental studies of the process science, and a viable technology to support real-time control of aerosol jet printing. 1. An aerosol jet printer , comprising:an atomizer for atomization of ink droplets, thereby providing an aerosol stream of aerosolized ink droplets;an optical measurement system, comprising a light source, an optics cell having a region for interaction of light from the light source with the aerosol stream, and a light sensor for detecting the light scattered from or transmitted through the aerosol stream in the interaction region, thereby providing an optical measurement of the aerosol stream; anda printhead downstream from the optical measurement system for printing of the ink droplets on a substrate.2. The aerosol jet printer of claim 1 , further comprising a closed-loop controller that uses a process metric to control at least one process parameter in real time.3. The aerosol jet printer of claim 2 , wherein the process metric comprises the product of an aerosol flow rate and an optical extinction of the aerosol stream.4. The aerosol jet printer of claim 2 , wherein the at least one process parameter comprises an atomizer voltage claim 2 , duty cycle claim 2 , temperature claim 2 , cartridge fill level claim 2 , ink composition claim 2 , aerosol flow rate claim 2 , sheath gas flow rate claim 2 , or ...

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27-01-2022 дата публикации

NUCLEIC ACID SEQUENCING SYSTEMS

Номер: US20220025457A1
Принадлежит:

Methods and systems for sequencing a nucleic acid molecule are described that comprise imaging a first surface and an axially-displaced second surface using a compensation-free optical system, the system comprising an objective lens and at least one image sensor, wherein said optical system has a numerical aperture (NA) of less than 0.6 and a field-of-view (FOV) of greater than 1.0 mm; and) processing the images of the first surface and the axially-displaced second surface to correct for optical aberration such that the images of the first surface and the axially-displaced second surface have substantially the same optical resolution. 1. A system for sequencing a nucleic acid molecule comprising:a) an optical system comprising an objective lens and at least one image sensor, wherein said optical system has a numerical aperture (NA) of less than 0.6, and is configured to acquire images of a first surface and an axially-displaced second surface; and i) process images of the first surface and the axially-displaced second surface to correct for optical aberration such that the images of the first surface and the axially-displaced second surface have substantially the same optical resolution; and', 'ii) detect a fluorescently-labeled composition comprising the nucleic acid molecule, or a complement thereof, disposed on the first surface or the axially-displaced second surface to determine an identity of a nucleotide in the nucleic acid molecule., 'b) a processor programmed to2. The system of claim 1 , wherein the images of the first surface and the axially-displaced second surface are acquired without moving an optical compensator into an optical path between said objective lens and said at least one image sensor.3. The system of claim 1 , wherein the images of the first surface and the axially-displaced second surface are acquired by just refocusing the optical system.4. The system of claim 1 , wherein the numerical aperture is greater than 0.3.5. The system of claim 1 ...

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27-01-2022 дата публикации

Complex particle measurement apparatus

Номер: US20220026330A1
Принадлежит: Horiba Ltd

A complex particle measurement apparatus comprising a first light source that irradiates a first storage cell; a photodetector that detects intensity of light; a second light source that irradiates a second storage cell; an imaging unit that images a particle group; an image data output unit that outputs image data; a supporter that supports the first storage cell and the second storage cell; and a communication pipe that connects the first storage cell and the second storage cell to pass a sample solution, wherein the first storage cell and the second storage cell have bottom surfaces located at positions different from each other, and the communication pipe is laid such that a channel from the first storage cell to the second storage cell has an incline of not less than 0 or not more than 0.

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27-01-2022 дата публикации

METHOD FOR DETECTING PARTICLES OR AEROSOL IN A FLOWING FLUID, COMPUTER PROGRAM, AS WELL AS ELECTRICAL MEMORY MEDIUM

Номер: US20220026338A1
Автор: Rusanov Radoslav
Принадлежит:

A method for detecting particles or aerosol in a flowing fluid, using the principle of laser-induced incandescence. The method includes the following steps: a. focusing a laser light originating from a laser in a spot; b. conducting a fluid which includes particles or aerosol through the spot; c. detecting a thermal radiation originating from the spot with the aid of a detector; and d. evaluating a variable which is provided by the detector and characterizes the detected thermal radiation within time intervals, the duration of the time intervals being dependent on a velocity of the fluid. 114-. (canceled)15. A method for detecting particles or aerosol in a flowing fluid , using laser-induced incandescence , the method comprising the following steps:a. focusing a laser light originating from a laser in a spot;b. conducting the fluid which includes particles or aerosol through the spot;c. detecting a thermal radiation originating from the spot using a detector; andd. evaluating a variable which is provided by the detector and characterizes the detected thermal radiation within time intervals, a duration of the time intervals being dependent on a velocity of the fluid.16. The method as recited in claim 15 , wherein at least several of the time intervals overlap.17. The method as recited in claim 16 , wherein the duration of the time intervals is greater than an expected full width at half maximum (FWHM) of the variable characterizing the thermal radiation.18. The method as recited in claim 17 , wherein the duration of the time intervals is 1 to 2 times the expected FWHM.19. The method as recited in claim 18 , wherein the duration of the time intervals is 1.5 times the expected FWHM.20. The method as recited in claim 16 , wherein an overlapping time period of the time intervals corresponds to at least half the duration of the time interval.21. The method as recited in claim 16 , wherein a particle is considered to be detected when the variable characterizing the thermal ...

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27-01-2022 дата публикации

METHODS AND DEVICES FOR EVALUATING PERFORMANCE OF A DIODE LASER

Номер: US20220026339A1
Принадлежит:

Methods for evaluating performance a diode laser are provided. In embodiments, methods include receiving a laser beam profile of a diode laser, determining first, second and third laser beam widths at first, second and third laser intensities, respectively, for the laser beam profile, computing a first ratio between the second and third laser beam widths, computing a second ratio between the first and second laser beam widths, evaluating laser performance based on the first and second ratios, and outputting a determination regarding the suitability of the laser for use in a flow cytometry setting. Devices for practicing the subject methods are also provided, and include first and second stages configured to receive a diode laser and beam profiler, respectively. Aspects of the invention further include flow cytometers incorporating a diode laser that has been evaluated by the subject method. 1. A method for evaluating performance of a diode laser , the method comprising:receiving a laser beam profile of the diode laser, the laser beam profile comprising laser beam width data and laser intensity data;determining first, second and third laser beam widths at first, second and third laser intensities, respectively, for the laser beam profile;computing:a first ratio between the second and third laser beam widths; anda second ratio between the first and second laser beam widths; andevaluating laser performance based on the first and second ratios.2. The method according to claim 1 , wherein the diode laser is a semiconductor laser diode.35-. (canceled)6. The method according to claim 1 , wherein evaluating performance of the diode laser comprises assessing the extent to which the laser beam profile deviates from a Gaussian beam shape.7. The method according to claim 6 , wherein assessing the extent to which the laser beam profile deviates from a Gaussian beam shape comprises identifying whether multiple modes are present in the laser beam profile.8. The method according to ...

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12-01-2017 дата публикации

METHOD AND DEVICE FOR DETERMINING CHARACTERISTIC PROPERTIES OF A TRANSPARENT PARTICLE

Номер: US20170010197A1
Принадлежит:

The invention relates to a method for determining the size d of a transparent particle, according to which method the particle is illuminated with light from a light source, a radiation detector measures a time-resolved intensity profile of light of the light source scattered by the particle, a reflection peak () and a refraction peak are determined in the intensity profile and the size d of the particle is determined based on a time difference between the reflection peak () and the refraction peak. The method according to the invention is characterized in that the time-resolved intensity profile is measured at a definable scattering angle θs, a first second-order refraction peak () and a second second-order refraction peak () having a mode different from that of the first refraction peak () being determined, a characteristic variable γ being determined as the ratio of a first time difference Δtbetween the reflection peak () and the first refraction peak () and of a second time difference Δtbetween the reflection peak () and the second refraction peak (), and the size of only those particles being determined for which the characteristic variable γ corresponds to a definable value. 1. A method for determining characteristic properties of a transparent particle , wherein the particle is illuminated with light from a light source , wherein a time-resolved intensity profile of light from the light source that is scattered at the particle is measured by a radiation detector at a predefinable scattering angle θ , wherein characteristic scattered light peaks are determined in the intensity profile , and wherein a size of the particle is determined based on a time difference between two scattered light peaks ,wherein a first time difference is determined between a first pair of scattered light peaks and a second time difference is determined between a second pair of scattered light peaks, a characteristic variable is determined from the ratio of the first time difference ...

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12-01-2017 дата публикации

ANALYSIS DEVICE AND ANALYSIS METHOD

Номер: US20170010260A1
Принадлежит:

An analysis device optically scans a surface of a substrate to which particles are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the particles based on pulse interval between two pulse waves each having pulse width less than first reference value determined depending on first pulse width when the optical scanning unit scans a plurality of particles adjacent to each other when the two pulse waves are detected consecutively. 1. An analysis device comprising:an optical scanning unit configured to optically scan a surface of a substrate to which particles are fixed;a pulse detector configured to detect a pulse wave and a pulse width of the pulse wave included in a detection signal obtained from the optical scanning unit when the optical scanning unit scans the substrate; anda counting unit configured to count the particles based on a pulse interval between two pulse waves each having a pulse width less than a first reference value when the pulse detector consecutively detects the two pulse waves.2. The analysis device according to claim 1 , wherein claim 1 , when the two pulse waves each having the pulse width less than the first reference value are consecutively detected by the pulse detector claim 1 , the counting unit counts the particles based on a number obtained by dividing the pulse interval between the two pulse waves by a first pulse width detected when the optical scanning unit scans the particles adjacent to each other.3. The analysis device according to claim 2 , wherein the first reference value is a sum of the first pulse width and a predetermined value included in the detection signal.4. The analysis device according to claim 1 , wherein the counting unit counts the particles and determines that a particle count is one when the pulse detector detects a pulse wave having a pulse width greater than or equal to the first reference value and less ...

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14-01-2016 дата публикации

Flow Cytometry Apparatus and Methods

Номер: US20160011096A1
Автор: Vacca Giacomo
Принадлежит:

A particle analyzer, comprising a source of a substantially nondiffracting light beam; a flow path configured to produce in a flowcell a ribbon-like core stream having a cross-sectional aspect ratio of at least 4 and a largest cross-sectional dimension of at least 50 micrometers; the flowcell being configured to expose a segment of the core stream to the light beam; a detector configured to receive a signal resulting from an interaction of a particle in the core stream with the light beam; a first sorting actuator connected with the flowcell, downstream of the exposed segment of core stream; a plurality of sorting channels in fluid connection with the flow path and downstream of the first actuator; the actuator having multiple actuation states, each state configured to direct at least one part of the core stream to a corresponding channel; a second sorting actuator connected with the flowcell, opposite the first actuator, and operable in coordination with the first actuator. 1. A particle analyzer , comprising:a source of a non-Gaussian, substantially nondiffracting light beam;a flow path configured to produce a ribbon-like core stream in a flowcell, said core stream having a cross-sectional aspect ratio of at least 4 and a largest cross-sectional dimension of at least 50 micrometers;said flowcell being configured to expose a segment of said core stream to said light beam; anda detector configured to detect a signal from said core stream, the signal resulting from an interaction of a particle in said core stream with said light beam.2. The particle analyzer of claim 1 , further comprising:a first sorting actuator connected with said flowcell and downstream of said segment of said core stream exposed to said light beam; anda plurality of sorting channels in fluid connection with said flow path and downstream of said first sorting actuator;said first sorting actuator having multiple actuation states, each actuation state configured to direct at least one part of said ...

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14-01-2016 дата публикации

COMPOUND OPTICAL FLOW CELLS AND METHOD OF MANUFACTURE AND USE

Номер: US20160011098A1
Принадлежит:

An improved optical flow cell adapted for use in a flow cytometer for differentiating formed bodies (e.g., blood cells) in liquid suspensions. Preferably manufactured by assembling, aligning, and optically joining at least two elements made from transparent material, the improved flow cell has a seamless internal flow channel of preferably non-circular cross-section in a cylindrical first element through which prepared samples can be metered and an independent second element having an external envelope suited to acquisition of optical parameters from formed bodies in such suspensions, the second element being conforming and alignable to the first element so that non-axisymmetric refractive effects on optical characterizing parameters of formed bodies passing through the flow channel in the first element may be minimized before the two elements are optically joined and fixed in working spatial relationship. 1. A method for making a transparent compound optical flow cell of the type used to characterize formed bodies passing through the flow cell , the optical flow cell having formed therein a rectilinear internal flow channel , the method comprising the steps of:providing a cylindrical monolithic preform comprising a thick-wall glass tube having an axially-extending channel therethrough and a transition temperature, the channel comprising a substantially uniform original cross-section of a desired shape;heating the preform to a predetermined temperature above the transition temperature of the glass tube;axially drawing the preform at a controlled rate, for a controlled time, and at a constant angular orientation, to achieve a desired reduced cross-sectional area of the axially-extending channel;providing an optical element, the optical element comprising a conforming surface that conforms to a segment of the drawn preform, and an exterior non-cylindrical envelope of predetermined form and orientation relative to the conforming surface;assembling the optical element ...

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11-01-2018 дата публикации

OPTICAL PARTICLE SORTER

Номер: US20180010997A1
Принадлежит:

A process for optically sorting a plurality of particles includes: providing a particle receiver; producing particles; receiving the particles by the particle receiver; receiving a light by the particle receiver; producing a standing wave optical interference pattern in an optical interference site of the particle receiver from the light; subjecting the particles to an optical gradient force from the standing wave optical interference pattern; deflecting the particles into a plurality of deflected paths to form the sorted particles from the particles; and propagating the sorted particles from the optical interference site through the deflected paths to optically sort the particles 1. An optical particle sorter comprising: a particle entrance that receives a plurality of particles;', 'an optical entrance that receives light and that is geometrically disposed at a non-parallel angle with respect to the particle entrance;', 'a sorted particle exit opposing the particle entrance and that communicates sorted particles from an optical interference site; and', 'the optical interference site interposed between the particle entrance and the sorted particle exit;, 'a particle receiver comprising produces a first light; and', 'produces a standing wave optical interference pattern in the optical interference site of the particle receiver; and, 'a first light source in optical communication with the particle receiver and that provides the particles; and', {'b': '24', 'communicates the particles to the particle receiver at an acute angle with respect to the standing wave optical interference pattern ,'}], 'a particle source in fluid communication with the particle receiver and thatwherein the optical particle sorter sorts the particles into a plurality of sorted particles that exit the particle receiver at the sorted particle exit, andthe sorted particles propagate in a plurality of deflected paths relative to a path of propagation of the particles at the particle entrance, the ...

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11-01-2018 дата публикации

EVALUATING BIOLOGICAL MATERIAL FOR UNASSOCIATED VIRUS-SIZE PARTICLES WITH ADENOVIRUS OR ADENO-ASSOCIATED VIRUS EPITOPE

Номер: US20180010998A1
Принадлежит:

A method for evaluating a biological material for unassociated virus-size particles having a particular epitope indicative of an adeno-associated virus viral type or an adenovirus viral type uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the virus-size particles and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such a virus-size particle and fluorescent antibody stain. 1. A flow cytometry method for evaluating a biological material sample for unassociated virus-size particles of a viral type selected from the group consisting of an adenovirus viral type and an adeno-associated virus viral type , the method comprising: flowing the fluid sample through a flow cell of a flow cytometer;', 'subjecting the fluid sample flowing through the flow cell to excitation radiation capable of causing a fluorescent emission response from the fluorescent antibody stain; and', 'detecting radiation from the flow cell within a wavelength range of the fluorescent emission and evaluating the detected radiation to identify detection events indicative of passage through the flow cell of the unassociated labeled particles of virus size including a said virus-size particle of the viral type bound with a portion of the fluorescent antibody stain;, 'subjecting to flow cytometry a fluid sample comprising at least a portion of the biological material sample, wherein the fluid sample comprises a fluorescent antibody stain capable of binding, directly or indirectly, with an epitope of the viral type, the flow cytometry comprisingwherein the unassociated labeled particles of virus size are of a particle size in a range of from 10 nanometers to 200 nanometer; andwherein the fluid sample as fed to the flow cytometer comprises a concentration of the fluorescent ...

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11-01-2018 дата публикации

EVALUATING BIOLOGICAL MATERIAL FOR UNASSOCIATED VIRUS-LIKE PARTICLES

Номер: US20180010999A1
Принадлежит:

A method for evaluating a biological material for unassociated virus-like particles virus size having a particular epitope uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the virus-size particles and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such a virus-like particle and fluorescent antibody stain. 2. A method according to claim 1 , wherein the epitope is selected from the group consisting of a baculovirus epitope claim 1 , an adenovirus epitope claim 1 , an influenza virus epitope claim 1 , an enterovirus epitope claim 1 , an adeno-associated virus (AAV) epitope and a norovirus epitope.3. A method according to claim 1 , wherein the epitope is a first epitope claim 1 , the flow cytometry is first flow cytometry claim 1 , the fluid sample is a first fluid sample including a first portion of the of the biological material sample claim 1 , the unassociated labeled particles are first unassociated labeled particles and the excitation radiation is first excitation radiation claim 1 , and the method comprises: flowing the second fluid sample through a flow cell of a flow cytometer;', 'subjecting the second fluid sample flowing through the flow cell to second excitation radiation, which is the same as or different than the first excitation radiation, capable of causing a second fluorescent emission response that is different than the first fluorescent emission response from the second fluorescent antibody stain; and', 'detecting radiation from the flow cell within a wavelength range of the fluorescent emission and evaluating the detected radiation to identify detection events indicative of passage through the flow cell of second unassociated labeled particles of virus size including a particle of virus size having the second ...

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11-01-2018 дата публикации

Automated real-time particle characterization and three-dimensional velocimetry with holographic video microscopy

Номер: US20180011001A1
Принадлежит: New York University NYU

An in-line holographic microscope can be used to analyze on a frame-by-frame basis a video stream to track individual colloidal particles' three-dimensional motions. The system and method can provide real time nanometer resolution, and simultaneously measure particle sizes and refractive indexes. Through a combination of applying a combination of Lorenz-Mie analysis with selected hardware and software methods, this analysis can be carried out in near real time. An efficient particle identification methodology automates initial position estimation with sufficient accuracy to enable unattended holographic tracking and characterization.

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11-01-2018 дата публикации

SYSTEM AND METHOD FOR IMMERSION FLOW CYTOMETRY

Номер: US20180011016A1
Автор: Swalwell Jarred E.
Принадлежит: UNIVERSITY OF WASHINGTON

An immersion cytometry system () having a primary focusing optic immersed in a fluid stream () containing suspended particles (). The system includes a light source () configured to illuminate a sensing region in the fluid stream that includes a focus of the primary optic. Light scattered and/or fluoresced from suspended particles passing through the sensing region is focused by an external tube lens on an external detector. The primary optic in one embodiment is a ball lens. In some embodiments, one or more filter/beam splitters on the optical axis reflect a portion of the signal light towards corresponding detectors, each filter being configured to reflect a preselected waveband of light. 1. A flow cytometry system for detecting particles suspended in a fluid stream , the system comprising:a light source configured to direct light through a sensing region in the fluid stream;a primary focusing optic at least partially immersed in the fluid stream and positioned to receive and focus light scattered by the suspended particles when they are in the sensing region; anda tube lens positioned to receive light from the primary optic and to focus the received light onto a first photo detector, the primary optic and the tube lens defining an optical axis that is aligned with the photo detector;wherein the first photo detector is configured to generate a signal responsive to the light focused by the tube lens onto the first photo detector and to transmit the generated signal to a processing system.2. The immersion flow cytometry system of claim 1 , wherein the primary focusing optic comprises a ball lens.3. The immersion flow cytometry system of claim 2 , wherein the ball lens comprises glass.4. The immersion flow cytometry system of claim 3 , wherein the glass is a flint glass.5. The immersion flow cytometry system of claim 1 , wherein the primary focusing optic is configured to chromatically distort the light claim 1 , such that the location of the focus plane on the ...

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14-01-2021 дата публикации

Air bubble measurement device and air bubble measurement method

Номер: US20210010917A1
Автор: Shintaro Ishikawa
Принадлежит: SUMITOMO METAL MINING CO LTD

An air bubble measurement device is a device that measures the air bubbles moving in the liquid. The air bubble measurement device includes a measurement chamber that holds a liquid. The measurement chamber includes an introduction port to introduce the air bubbles in the liquid from a lower side and a transparent inclined surface that faces obliquely downward and is disposed at a position to which the air bubbles present inside the liquid move up. The transparent inclined surface includes a hydrophilic membrane. The hydrophilic membrane has a contact angle with water of 20 degrees or less. This structural arrangement allows for reducing an attachment of the air bubbles on the transparent inclined surface even when the air bubbles become small. This allows for reducing stay of the air bubbles on the transparent inclined surface and allows for accurately measuring the states of the air bubbles (that is, the size and quantity of the air bubbles).

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14-01-2021 дата публикации

FLOW CYTOMETER AND PARTICLE DETECTION METHOD

Номер: US20210010925A1
Принадлежит: SYSMEX CORPORATION

A flow cytometer, in which detection of light generated from a particle is less likely to be affected by change in a flow velocity of a liquid flowing in a flow cell, is provided. The flow cytometer includes: a flow cell () in which a liquid flows; a liquid sending unit () configured to send the liquid into the flow cell (); a controller () configured to obtain information related to a flow velocity of the liquid flowing in the flow cell (); a light source () configured to irradiate the liquid flowing in the flow cell () with light; and a detector () configured to detect light generated from a particle in the liquid irradiated with light. The controller () changes a liquid sending condition for the liquid sending unit (), based on the obtained information related to the flow velocity. 1. A flow cytometer comprising:a flow cell in which a liquid flows;a liquid sending unit configured to send the liquid into the flow cell;a controller configured to obtain information related to a flow velocity of the liquid flowing in the flow cell;a light source configured to irradiate the liquid flowing in the flow cell with light; anda detector configured to detect light generated from a particle in the liquid irradiated with light, whereinthe controller changes a liquid sending condition for the liquid sending unit, based on the obtained information related to the flow velocity.2. The flow cytometer according to claim 1 , wherein the controller further changes a detection condition for the detector claim 1 , based on the obtained information related to the flow velocity.3. The flow cytometer according to claim 2 , wherein the controller selectively changes the liquid sending condition for the liquid sending unit or the detection condition for the detector claim 2 , based on the obtained information related to the flow velocity.4. The flow cytometer according to claim 2 , whereinthe controllerchanges the liquid sending condition for the liquid sending unit when a difference between ...

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10-01-2019 дата публикации

OPTICAL MEASUREMENT APPARATUS, AND OPTICAL MEASUREMENT METHOD

Номер: US20190011351A1
Принадлежит:

An optical measurement apparatus includes a main body base, an optical base movably combined with the main body base, a measurement optical system fixed to the optical base, and an optical base moving mechanism which moves the optical base relative to the main body base. The optical base moving mechanism moves the optical base relative to the main body base between an internal measurement position and an external measurement position. A measurement object position of the measurement optical system coincides with an internal measurement object position within the main body base. The measurement object position of the measurement optical system coincides with an external measurement object position outside the main body base. 1. An optical measurement apparatus comprising:a main body base;an optical base movably combined with the main body base;a measurement optical system fixed to the optical base; andan optical base moving mechanism which moves the optical base relative to the main body base between an internal measurement position defined such that a measurement object position of the measurement optical system coincides with an internal measurement object position within the main body base and an external measurement position defined such that the measurement object position of the measurement optical system coincides with an external measurement object position outside the main body base.2. The optical measurement apparatus according to claim 1 , further comprising a sample stage which is supported by the main body base and retains a sample holder to hold a sample claim 1 , wherein the internal measurement object position corresponds to a position of the sample held by the sample holder.3. The optical measurement apparatus according to claim 2 , further comprising a sample stage moving mechanism which moves the sample stage relative to the main body base between a measurement stage position defined such that the sample held by the sample holder is located at the ...

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14-01-2021 дата публикации

ADVANCED BIOPHYSICAL AND BIOCHEMICAL CELLULAR MONITORING AND QUANTIFICATION USING LASER FORCE CYTOLOGY

Номер: US20210011018A1
Принадлежит:

The present invention is directed to intelligent algorithms, methodologies and computer-implemented methodologies for biophysical and biochemical cellular monitoring and quantification enabling enhanced performance and objective analysis of advanced infectivity assays including neutralization assays and adventitious agent testing using fluidic and optical force-based measurements. 1. A method for measuring cellular responses to differential stimuli using optical and/or fluidic forces , wherein the method comprises:receiving a selection of an initial samples comprising biological cells treated with varying known levels of stimuli or analyte,performing optical force-based measurements on the samples,developing a response metric (RM) to describe the cellular response to the stimuli based on one or more optical or fluidic force-based parameters.2. The method of claim 1 , wherein the response metric is used to measure the response of additional unknown samples.3. The method of claim 1 , further comprising analyzing dilutions of the sample until an accurate measurement of the infectivity is determined claim 1 , based upon having an RM that falls within the acceptable target value range.4. (canceled)5. The method of claim 1 , where the optical and fluidic forces are based on laser force cytology.6. The method of further comprising:comparing the response metric of an initial sample to a target value;selecting a second sample based on the results of the first and an algorithm governing the expected or known response;comparing the response metric of the second sample to a target value; andselecting subsequent samples in a similar manner until a sample matching the target response metric or other defined endpoint is identified.7. The method of claim 5 , wherein the optical force-based measurements utilize laser force cytology to assess parameters comprising linear velocity claim 5 , size claim 5 , perimeter claim 5 , size (area claim 5 , diameter claim 5 , volume claim 5 , etc ...

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14-01-2021 дата публикации

Method for detection of cells in a cytological sample having at least one anomaly

Номер: US20210012088A1

Disclosed is a method for detecting cells having at least one anomaly in a cytological sample on the basis of at least one first digitised digitised-electron-microscopy image of the sample.

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03-02-2022 дата публикации

MICROPARTICLE MEASURING APPARATUS AND MICROPARTICLE MEASURING METHOD

Номер: US20220034784A1
Автор: Kato Yasunobu
Принадлежит:

To provide a technology of maintaining light detection accuracy at a high level irrespective of individual variations in flow rate of microparticles flowing through a flow channel. The present technology provides a microparticle measuring apparatus including: a plurality of light detection sections configured to detect, at different positions, optical information emitted from microparticles flowing through a flow channel; and a detection timing control section configured to control a detection timing of each light detection section, on the basis of a trigger signal detected at a first reference channel provided in a first light detection section, and an optical signal detected at a second reference channel provided in a second light detection section that detects optical information emitted from the microparticles, at a position different from a position of the first light detection section. 1. A microparticle measuring apparatus comprising:a plurality of light detection sections configured to detect, at different positions, optical information emitted from microparticles flowing through a flow channel; anda detection timing control section configured to control a detection timing of each light detection section, on a basis of a trigger signal detected at a first reference channel provided in a first light detection section, and an optical signal detected at a second reference channel provided in a second light detection section that detects optical information emitted from the microparticles, at a position different from a position of the first light detection section.2. The microparticle measuring apparatus according to claim 1 , whereinthe detection timing control section controls the detection timing of each light detection section in real time.3. The microparticle measuring apparatus according to claim 1 , whereinthe detection timing control section controls a detection process period of the second light detection section.4. The microparticle measuring ...

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03-02-2022 дата публикации

CELL SORTING DEVICE AND METHOD

Номер: US20220034785A1
Автор: Gu Yi, Lo Yu-Hwa
Принадлежит:

A cell sorting system is provided to comprise: an imaging device including abeam scanner scanning abeam along a first direction to obtain a cell image data including fluorescent information or cell image information of a cell, the beam applied to the cell flowing in a channel along a second direction with an angle to the first direction; a data processing and control device in communication with the imaging device, the data processing and control device including a processor configured to process the cell image data obtained by the imaging device to determine one or more properties associated with the cell from the processed cell image data and to produce a control command based on a comparison of the determined one or more properties with a sorting criteria, and a cell sorting device in communication with the imaging device and the data processing and control device. 1. A cell sorting system , comprising:an imaging device including a beam scanner scanning a beam along a first direction to obtain a cell image data including fluorescent information or cell image information of a cell, the beam applied to the cell flowing in a channel along a second direction with an angle to the first direction;a data processing and control device in communication with the imaging device, the data processing and control device including a processor configured to process the cell image data obtained by the imaging device to determine one or more properties associated with the cell from the processed cell image data and to produce a control command based on a comparison of the determined one or more properties with a sorting criteria, wherein the control command is produced during the cell flowing in the channel and is indicative of a sorting decision determined based on one or more cellular attributes ascertained from the cell image data; anda cell sorting device in communication with the imaging device and the data processing and control device, the cell sorting device including two ...

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19-01-2017 дата публикации

Bio Sensor and Air Cleaner Having Same

Номер: US20170016638A1
Автор: Seong-Jin Yun
Принадлежит: Coway Co Ltd

There is provided a biosensor, which may measure the concentration of indoor bioaerosols through an optical sensing method of sensing ultraviolet light scattered by bioaerosols, and an air cleaner having the same. The biosensor includes a light irradiator and an ultraviolet light sensor detecting scattered ultraviolet light, from light irradiated by the light irradiator, reflected from bioaerosols. The biosensor may measure the concentration of indoor bioaerosols through the optical sensing method in real time, using a certain wavelength of ultraviolet light scattered by bioaerosols, and may allow the air cleaner to be operated under proper conditions based on a measured concentration of bioaerosols and a measured concentration of dust particles.

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19-01-2017 дата публикации

SCANNING INFRARED MEASUREMENT SYSTEM

Номер: US20170016813A1
Автор: Wagner Matthias
Принадлежит:

An analyzer of a component in a sample fluid includes an optical source and an optical detector defining a beam path of a beam, wherein the optical source emits the beam and the optical detector measures the beam after partial absorption by the sample fluid, a fluid flow cell disposed on the beam path defining an interrogation region in the a fluid flow cell in which the optical beam interacts with the sample fluid and a reference fluid; and wherein the sample fluid and the reference fluid are in laminar flow, and a scanning system that scans the beam relative to the laminar flow within the fluid flow cell, wherein the scanning system scans the beam relative to both the sample fluid and the reference fluid. 1. An analyzer of a component , comprising:an optical source that emits a light;a scanning system that scans the light over the component;an optical detector subsystem that measures the light after interaction with the component; anda guiding system that guides the light from the optical source to the optical detector subsystem.2. The analyzer of claim 1 , further comprising an interrogation region disposed on a beam path defined between the optical source and the optical detector subsystem claim 1 , which is a closed claim 1 , liquid-based environment at the time of interrogation and contains the component; wherein the component is a particle or a cell; and a processor that calculates a characteristic of the component based on the measured light.3. The analyzer of claim 1 , wherein the optical detector subsystem is configured to respond preferentially to light at the scanning frequency to measure transmitted light as the light passes through the component or to measure scattered light associated with as the light passes by the component.4. The analyzer of claim 1 , wherein the optical source is a quantum cascade laser (QCL) which emits the light of at least one wavelength.5. The analyzer of claim 1 , wherein the optical source emits the light of mid-infrared or ...

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21-01-2016 дата публикации

White Blood Cell Analysis System and Method

Номер: US20160018310A1
Автор: Vacca Giacomo, Wu Jiong
Принадлежит:

Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WB Cs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.

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21-01-2016 дата публикации

Basophil Analysis System and Method

Номер: US20160018311A1
Принадлежит:

Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.

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21-01-2016 дата публикации

Nucleated Red Blood Cell Analysis System and Method

Номер: US20160018312A1
Принадлежит:

Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs. 1. A hematology analyzer for conducting a nucleated red blood cell (nRBC) analysis on a blood sample that contains a plurality of nRBCs , the analyzer comprising:an excitation source positioned to excite particles within the blood sample;a plurality of detectors including (1) an axial light loss detector positioned to measure axial light loss from the excited blood sample, (2) an intermediate angle scatter detector positioned to measure intermediate angle scatter from the excited blood sample, (3) a side scatter detector positioned to measure 90° side scatter from the excited blood sample, and (4) a fluorescence detector positioned to measure fluorescence emitted from the excited blood sample; and (a) dilute the blood sample with a reagent that includes a red blood cell (RBC) lysing agent and a cell membrane permeable, nucleic acid binding fluorescent dye;', '(b) incubate the diluted blood sample of step (a) for an incubation period of time', '(c) deliver the incubated sample from step (b) to a flow cell in the hematology analyzer;', '(d) excite the incubated ...

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21-01-2016 дата публикации

Method for Hematology Analysis

Номер: US20160018313A1
Автор: Vacca Giacomo, Wu Jiong
Принадлежит:

A method whereby one or more fluorescent dyes are used to bind and stain nucleic acids in certain blood cells, such as, for example, white blood cells, nucleated red blood cells, and reticulocytes, and to induce fluorescent emissions upon excitation of photons from a given source of light, such as, for example, a laser, at an appropriate wavelength. More particularly, this invention provides a method whereby a fluorescent trigger is used in a data collection step for collecting events that emit strong fluorescence, in order to separate white blood cells and nucleated red blood cells from red blood cells and platelets without the need for using a lysing agent.

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21-01-2016 дата публикации

PARTICLE ANALYSIS APPARATUS

Номер: US20160018314A1
Принадлежит:

A particle analysis apparatus for flow cytometry, which contains a flow cell having a flow channel for flowing a sample solution containing particles to be analyzed, a light source device for emitting an irradiation light, an optical system for irradiating the irradiation light on an irradiation segment in the flow channel, and a light receiving device for detecting the light obtained thereby. A light source of the light source device is LED, and an electrode formed on a light extraction surface thereof mainly contains a plurality of electric conductor lines arranged in parallel to each other.

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21-01-2021 дата публикации

METHODS AND APPARATUSES FOR SORTING TARGET PARTICLES

Номер: US20210016277A1
Принадлежит:

This disclosure provides methods and apparatuses for sorting target particles. In various embodiments, the disclosure provides a cassette for sorting target particles, methods for sorting target particles, methods of loading a microchannel for maintaining sample material viability, methods of quantifying sample material, and an optical apparatus for laser scanning and particle sorting. 1165.-. (canceled)166. A method of sorting cells , comprising screening cellular material to identify cells with a desired phenotype , wherein the cellular material is screened at a rate of 50 ,000 cells per second or greater.167. The method of claim 166 , wherein the cellular material is screened at a rate 500 claim 166 ,000 cells per second or greater.168. The method of claim 166 , wherein the cellular material is screened at a rate of 1 claim 166 ,000 claim 166 ,000 cells per second or greater.169. The method of claim 166 , wherein the method further comprises extracting said cells of a desired phenotype from said cellular material at a rate of 100 claim 166 ,000 cells per second or greater.170. The method of claim 169 , wherein said cells of a desired phenotype are extracted at a rate of 300 claim 169 ,000 cells per second or greater.171. The method of claim 166 , wherein said cellular material is screened in an array with through-holes.172. The method of claim 171 , wherein said cells of aid desired phenotype are extracted from said array.173. The method of claim 172 , wherein said extracting comprises releasing said cells of said desired phenotype using electromagnetic radiation.174. The method of claim 173 , wherein each through-hole of said array comprises from 0 to about 5 cells and at least 30% of the through-holes of the array comprise at least one cell.175. The method of claim 166 , wherein the cellular material is obtained from a human subject and/or comprises less than 5% of HSCs and HSPCs.176. The method of claim 169 , wherein greater than 95% of the extracted cells are ...

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18-01-2018 дата публикации

URINE ANALYSIS SYSTEM, IMAGE CAPTURING APPARATUS, URINE ANALYSIS METHOD

Номер: US20180017480A1
Принадлежит: SYSMEX CORPORATION

A urine analysis system according to an embodiment includes: a testing apparatus that measures particles included in a urine sample according to a flow cytometry method; an image capturing apparatus that captures images of particles in the urine sample to acquire particle images; and a management apparatus that receives a measurement result obtained by the testing apparatus and the particle images acquired by the image capturing apparatus. The management apparatus generates an order to capture an image of the urine sample based on the measurement result obtained by the testing apparatus. The image capturing apparatus executes the image capturing processing of the particles in the urine sample for which the image capturing order has been generated by the management apparatus, and transmits the acquired particle images to the management apparatus. 1. A urine analysis system comprising:a testing apparatus that measures particles included in a urine sample according to a flow cytometry method;an image capturing apparatus that captures images of the urine sample to acquire images of particles in the urine sample; anda management apparatus that receives a measurement result obtained by the testing apparatus and the particle images acquired by the image capturing apparatus, whereinthe management apparatus generates an image capturing order for the urine sample based on the measurement result obtained by the testing apparatus, and the image capturing apparatus executes the image capturing processing of the urine sample for which the image capturing order has been generated by the management apparatus, and transmits the acquired particle images to the management apparatus.2. The urine analysis system according to claim 1 , further comprising a conveyance apparatus that conveys the urine sample from the testing apparatus to the image capturing apparatus based on the image capturing order claim 1 , whereinthe image capturing apparatus includes a suction unit that sucks the ...

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18-01-2018 дата публикации

ACHROMATIC ANASTIGMATIC ANAMORPHIC OBJECTIVE

Номер: US20180017769A1
Принадлежит:

In a flow cytometer, an objective lens for focusing an input laser-radiation beam including at least four different laser-radiation wavelengths in a common plane includes only three singlet lens-elements. Two of the elements are cylindrical elements arranged as a cylindrical telescope for shaping and reducing the size of the input laser-beam. The third element is a spherical element arranged to focus the reduced size beam in the common plane. In one example, all three elements are made from the same optical material. 1. An objective lens for focusing an input beam of laser-radiation in a focal plane , comprises:first, second, and third optical elements in consecutive numerical order in a propagation direction of the beam;{'sub': 'CL1', 'the first optical element being a singlet cylindrical element having a focal length f;'}{'sub': 'CL2', 'the second optical element being a singlet cylindrical element having a focal length f;'}{'sub': 'FFL', 'the third optical element being a singlet rotationally symmetrical element having a focal length f; and'}{'sub': CL1', 'CL2', 'FFL, 'claim-text': {'br': None, 'i': f', 'f', 'G', 'f, 'sub': CL1', 'CL2', 'FFL, '−=*'}, 'wherein f, f, and fare related by an equation'}where G is between about 0.7 and about 1.4.2. The objective lens of claim 1 , wherein G is between about 0.9 and 1.1.3. The objective lens of claim 1 , wherein the third element is a spherical element.4. The objective lens of claim 1 , wherein each of the lens elements has a plane surface and a curved surface.5. The objective lens of claim 4 , wherein the curved surface of each of the optical elements is the first surface of that element in the propagation direction of the beam.6. The objective lens of claim 1 , the first claim 1 , second claim 1 , and third optical elements are made from the same material.7. The objective lens of claim 6 , wherein the optical elements are made from fused silica.8. The objective lens of claim 6 , wherein the optical elements are made ...

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17-01-2019 дата публикации

Monitoring device for adjusting light irradiation in particle analysis apparatus

Номер: US20190017920A1
Автор: Masashi Nishimori
Принадлежит: Horiba Ltd

Provided is a monitoring device having a control part for monitoring, wherein the control part for monitoring has a first data processing part, a second data processing part, and an indication value output part. The first data processing part generates, as indication values for optical axis adjustment, the first indication value showing the intensity of a light-receiving signal obtained from the particle analysis apparatus. The second data processing part generates, as indication values for gain adjustment of the light-receiving signal, the second indication value showing the intensity of a light-receiving signal obtained from the particle analysis apparatus, which is different from the aforementioned first indication value. The indication value output part outputs the first and second indication values to a display device.

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16-01-2020 дата публикации

OPTICAL PARTICLE SENSOR AND SENSING METHOD

Номер: US20200018683A1
Автор: VAN DER SLUIS Paul
Принадлежит:

An optical particle sensor comprises at least first and second light sources of different wavelength for sequential operation. An optical detector is used to detect light from the light sources emitted or scattered by particles to be sensed. A current injection compensation signal is also provided which is dependent on which light source of the optical arrangement is in use. The compensation signal means the amplifier does not need to re-settle in response to different background illumination levels associated with the different light sources. In this way, detection signals may be obtained in quick succession from different light sources. 1. An optical particle sensor , comprising:an optical arrangement comprising at least first and second light sources of different wavelengths for sequential operation;an optical detector for detecting light emitted from particles to be sensed or light from the light sources scattered by particles to be sensed and generating a detection current;an amplifier circuit for amplifying the detection current output from the optical detector;a compensation circuit for providing a current injection compensation signal to the amplifier circuit which combines with the detection current; anda controller, wherein the controller is adapted to provide a current injection compensation signal which is dependent on which light source of the optical arrangement is in use.2. A sensor as claimed in claim 1 , wherein the compensation circuit comprises a voltage source for controlling a level of current injection.3. A sensor as claimed in claim 2 , wherein the compensation circuit comprises a delay circuit for coupling the voltage from the voltage source to the amplifier circuit and generating the current injection compensation signal.4. A sensor as claimed in claim 3 , wherein the delay circuit comprises a low pass filter claim 3 , wherein the current injection compensation signal is provided from the output of the low pass filter to the output of the ...

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16-01-2020 дата публикации

Particle Detection Using Thin Lenses

Номер: US20200018684A1
Автор: Vercruysse Dries
Принадлежит:

An optical excitation system comprises a substrate () comprising at least one delivery means (), for delivering analytes () into at least one region of interest (), at least one radiation carrier () for directing at least one radiation beam from the at least one radiation carrier () into the at least one region of interest (). The substrate () includes a thin lens system () comprising at least a first thin lens (), for collimating radiation from the at least one region of interest () to a remote detection system (). A particle sensor and sensing system comprising the excitation system are also provided, for example a modular particle sensor and modular sensing system, wherein the optical excitation system may be single use and disposable. 1. An optical excitation system for a particle sensor , the system comprising: at least one delivery means for delivering analytes into at least one region of interest, and', 'at least one radiation carrier for carrying radiation and directing at least one radiation beam from the at least one radiation carrier into the at least one region of interest; and, '(a) a substrate comprising'}(b) a thin lens system comprising at least a first thin lens for collimating radiation from the at least one region of interest to a remote detection system.2. The optical excitation system according to claim 1 , wherein the at least a first thin lens is integrated in or on the substrate.3. The optical excitation system according to claim 1 , wherein the thin lens system comprises at least one thin film lens.4. The optical excitation system according to claim 1 , wherein the at least one delivery means comprises at least one microfluidic channel.5. A particle sensor comprising:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'an optical excitation system according to ; and'}a detection system comprising at least a first detector for detecting radiation within a predetermined range of wavelengths.6. The particle sensor according to claim 5 , wherein ...

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16-01-2020 дата публикации

SYSTEM AND METHOD OF LABEL-FREE CYTOMETRY BASED ON BRILLOUIN LIGHT SCATTERING

Номер: US20200018685A1
Принадлежит:

The present invention relates to a method and system for a label-free cell analysis based on Brillouin light scattering techniques. Combined with microfluidic technologies according to the present invention, Brillouin spectroscopy constitutes a powerful tool to analyze physical properties of cells in a contactless non-disturbing manner. Specifically, subcellular mechanical information can be obtained by analyzing the Brillouin spectrum of a cell. Furthermore, a novel configuration of Brillouin spectroscopy is provided to enable simultaneous analysis of multiple points in a cell sample. 1. A method for classifying biological cells , the method comprising:providing a container having a biological sample including the biological cells in a media;illuminating the biological sample to generate a Brillouin scattered light from within the biological cells and the media;measuring a Brillouin scattering spectrum at multiple points within each biological cell;extracting one or more metrics related to subcellular physical properties at different spatial points within the biological cells based on the measured Brillouin scattering spectrum; andclassifying the biological cells based on the subcellular physical properties at different spatial points within the biological cells.2. The method of claim 1 , wherein the one or more metrics associated with the Brillouin-scattering spectrum are selected from the group consisting of: Brillouin frequency shift claim 1 , Brillouin spectrum linewidth claim 1 , Brillouin gain or loss spectrum claim 1 , and a combination thereof.3. The method of claim 1 , wherein the physical properties of the sample are selected from the group consisting of: viscoelastic modulus claim 1 , density claim 1 , refractive index claim 1 , electrostriction claim 1 , and a combination thereof.4. The method of claim 1 , wherein the step of extracting subcellular physical properties at different spatial points within the biological cells further comprises:plotting a ...

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16-01-2020 дата публикации

MEASUREMENT OF SERUM LIPOPROTEINS

Номер: US20200018686A1
Автор: KULKARNI Krishnaji R.
Принадлежит:

Although a more accurate estimate of a person's risk of cardiovascular disease can be made on the basis of the number of lipoprotein particles per unit volume in the person's blood, current methods all rely on measuring the mass of lipoprotein cholesterol per unit volume. It has been discovered that a rapid and accurate lipoprotein particle count can be obtained by photometry. A method and apparatus are provided for measuring the number of lipoprotein particles in a sample using photometry. 1. A method of measuring lipoprotein particle count in a sample from a subject , the method comprising:a. introducing into an apparatus for measuring the lipoprotein particle number, a plurality of lipoprotein fractions comprising a LDL fraction, a HDL fraction, and a VLDL fraction, wherein each of the plurality of lipoprotein fractions are separated from one another;b. obtaining a photometric measurement in at least the LDL fraction; andc. calculating a particle count for the LDL fraction that is a function of the photometric measurement,wherein separation of the HDL fraction and the VLDL fraction is maintained during steps (a) to (c).2. The method of further comprising:a. obtaining a photometric measurement in the HDL fraction; andb. calculating a particle count for the HDL fraction that is a function of the photometric measurement.3. The method of further comprising:a. obtaining a photometric measurement in at least the VLDL fraction; andb. calculating a particle count for the VLDL fraction that is a function of the photometric measurement.4. The method of further comprising:a. obtaining a photometric measurement in at least the HDL fraction;b. calculating a particle count for the HDL fraction that is a function of the photometric measurement;c. obtaining a photometric measurement in at least the VLDL fraction; andd. calculating a particle count for the VLDL fraction that is a function of the photometric measurement.5. The method of claim 1 , wherein the plurality of ...

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16-01-2020 дата публикации

Environmental system on module apparatus

Номер: US20200018687A1
Принадлежит: Clad Innovations Ltd

An environmental system on a module apparatus for detecting and measuring particles entrained in an air stream. The apparatus includes a highly-integrated silicon circuit board. The circuit board has a plurality of embedded sensors, signal conditioning and processing. The plurality of sensors to detect pollution and other aspects of the physical environment. The apparatus also including a mechanical structure surrounding the top side of the circuit board. The structure forms an air pathway having an inlet and an outlet and a laser beam pathway having an emitter end and a beam line termination end. The air pathway intersects the laser beam pathway. A highly miniaturized optical particle scattering detector is located proximal to the intersection. A laser is located in the emitter end of the laser beam pathway.

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17-01-2019 дата публикации

ANALYSIS APPARATUS AND FOCUSING METHOD

Номер: US20190020825A1
Принадлежит: ARKRAY, INC.

An analysis apparatus comprises a flow cell which has a flow passage for a liquid containing a tangible component; an image pickupper which picks up images of the liquid flowing through the flow passage; an adjuster which adjusts a relative position of the flow cell with respect to the image pickupper in relation to an optical axis direction of the image pickupper and a direction in which the liquid flows through the flow passage; and a controller which determines an image pickup position of the flow cell in at least one of the optical axis direction and the direction in which the liquid flows, on the basis of a number of pieces of the tangible component in focusing states existing in the images of the liquid picked up by the image pickupper at a plurality of positions at which the relative position differs. 1. An analysis apparatus comprising:a flow cell which includes a flow passage for a liquid containing a tangible component;an image pickupper configured to pick up images of the liquid flowing through the flow passage;an adjuster configured to adjust a relative position of the flow cell with respect to the image pickupper in relation to an optical axis direction of the image pickupper and a direction in which the liquid flows through the flow passage; anda controller configured to judge focusing states of pieces of the tangible component existing in the images of the liquid picked up by the image pickupper at a plurality of positions at which the relative position differs, such that the controller configured to determine an image pickup position of the flow cell in at least one of the optical axis direction and the direction in which the liquid flows, on the basis of a number of the pieces of the tangible component judged to be in the focusing states.2. The analysis apparatus according to claim 1 , further comprising a light source for image pickup which emits light a plurality of times within an exposure time for one time of exposure performed by the image ...

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28-01-2016 дата публикации

FLOW CYTOMETER, PARTICLE ANALYZER, AND FLOW CYTOMETRIC METHOD

Номер: US20160025611A1
Принадлежит:

A flow cytometer comprises a flow cell configured to induce a flow of a sample containing object particles, a light source, an irradiating optical system configured to irradiate light from the light source on the flow of particles in the flow cell, a detecting part configured to detect light given off from the flow of particles which are irradiated by light. The irradiating optical system comprises a collective lens having an optical axis symmetric aspherical surface on one surface, and a cylindrical surface on the other surface. 1. A flow cytometer comprising:a flow cell configured to induce a flow of a sample containing object particles;a light source;an irradiating optical system configured to irradiate light from the light source on the flow of particles in the flow cell;a detecting part configured to detect light given off from the flow of particles which are irradiated by light;wherein the irradiating optical system comprises a collective lens having an optical axis symmetric aspherical surface on one surface, and a cylindrical surface on the other surface.2. The flow cytometer of claim 1 , wherein the collective lens is arranged so that the generating line of the cylindrical surface is parallel to the flow of the particles in the flow cell.3. The flow cytometer of claim 2 , wherein the optical axis symmetric aspherical surface is configured with a shape to condense the entering light at the focal point claim 2 ,the cylindrical surface is configured to shape the light irradiating the flow region so that the width in the direction parallel to the flow of particles in the flow cell is less than the width in the direction traversing the flow of the particles.4. The flow cytometer of claim 3 , wherein the optical axis symmetric aspherical surface is provided on the light entrance surface of the collective lens claim 3 , andthe cylindrical surface is provided on the light exit surface of the collective lens.5. The flow cytometer of claim 3 , wherein the optical ...

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