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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 11190. Отображено 100.
23-02-2012 дата публикации

Portable device based on immobilized cells for the detection of analytes

Номер: US20120045835A1
Принадлежит: Universita di Bologna

The present invention relates to a portable device for the detection of analytes, in particular toxic substances, comprising at least a whole-cell biosensor with cells immobilized onto a transparent and inert matrix that allows the maintenance of cell vitality. The matrix comprises a mixture of collagen and/or its enzyme degradation derivatives, a proteoglycan and a mixture of vinyl polymer and an optionally modified polysiloxane, and an orthosilicate. The biosensor can be a genetically modified cell that expresses luciferase as reporter gene.

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01-03-2012 дата публикации

Peptide-modified microcarriers for cell culture

Номер: US20120052579A1
Принадлежит: Corning Inc

A cell culture article including a microcarrier having a peptide-modified polymer surface of the formula (I) where AAj represents at least one covalently bonded peptide, j is an integer of from 5 to 50, m, n, o, Sur, X, R, R′, and the mer ratio (m-o:n:o), including salts thereof, are as defined herein. Also disclosed are methods for making and using the cell culture article, as defined herein.

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19-04-2012 дата публикации

Ex Vivo Cell Culture: Enabling Process and Devices

Номер: US20120094372A1

A device which is capable of providing an artificial condition for three dimensional culture of animal and human cells comprising of two components; First component is a vessel coated in the entire internal surface rendered not suitable for cell adhesion and proliferation and a second component is a cell interactive biomaterial on which cells can attach and grow as monolayer or multiple layers in three dimensions.

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03-05-2012 дата публикации

Aeration systems for horizontal photobioreactors

Номер: US20120107452A1
Принадлежит: Individual

This invention relates to an aeration system comprising an aeration section, a degassing section and optionally an additional section. This invention also relates to a horizontal photobioreactor comprising the aeration system. This invention further relates to methods of using the photobioreactors.

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24-05-2012 дата публикации

Honeycomb shrink wells for stem cell culture

Номер: US20120129208A1
Принадлежит: UNIVERSITY OF CALIFORNIA

This invention provides a microwell array having a plurality of microwells on a hydrophobic surface wherein the microwells each is substantially proximate to each of its adjacent microwells, as well as methods to prepare arrays. Also provided is a plate that comprises at least one microarray, at least one input channel, at least one output channel, and a channel connecting the input and output channel.

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31-05-2012 дата публикации

Bioreactor Comprising A Silicone Coating

Номер: US20120135514A1
Принадлежит: Wacker Chemie AG

The invention relates to a bioreactor for cultivating phototrophic organisms in an aqueous culture medium. The reactor parts and/or fittings that come into contact with the culture medium are entirely or partially coated with a silicone layer, and the surface of the silicone layer has a contact angle to the water of at least 100°.

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21-06-2012 дата публикации

Disposable vortex breaker

Номер: US20120155216A1
Принадлежит: EMD Millipore Corp

The present invention consists of a closed presterilized bag having a disposable mixing element within it, a drive mechanism outside of the bag for rotating the mixing element without voiding sterility and a vortex breaker in the torm of one or more plastic sheet materials that are attached to various inner surfaces of the bag and disrupt the formation of vortices within the bag. Preferably the sheet(s) are formed of the same material as the bag and are sealed to the bag surfaces. More preferably, the sheet(s) extend across a diameter of the bag. Most preferably, the sheet(s) are perforated with one or more slits or openings to allow for good flow and mixing without a vortex being formed.

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19-07-2012 дата публикации

Continuous culture apparatus with mobile vessel, allowing selection of fitter cell variants and producing a culture in a continuous manner

Номер: US20120184009A1
Принадлежит: Individual

A method and device for growing plant, animal or stem cells in a continuous manner.

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19-07-2012 дата публикации

Polymer coating comprising 2- methoxyethyl acrylate units synthesized by surface-initiated atom transfer radical polymerization

Номер: US20120184029A1
Принадлежит: Danmarks Tekniskie Universitet

The present invention relates to preparation of a polymer coating comprising or consisting of polymer chains comprising or consisting of units of 2-methoxyethyl acrylate synthesized by Surface-Initiated Atom Transfer Radical Polymerization (SI ATRP) such as ARGET SI ATRP or AGET SI ATRP and uses of said polymer coating.

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09-08-2012 дата публикации

Self-contained breathing closure and container

Номер: US20120199207A1
Принадлежит: Individual

A self-contained breathing closure for flasks and other containers that require gas exchange. An illustrative embodiment of the closure is comprised of a splashguard, an adaptor for attaching the closure to the container, a bellows element and a gas-permeable barrier element. The splashguard is intended to keep liquid contents under vigorous agitation in the container without wetting the gas-permeable barrier. The adaptor couples the closure to the container in a secure fashion. The bellows element allows for repeated changes in the internal volume of the container-closure system. The gas-permeable barrier allows desired gases to enter and leave the container while excluding small particles and/or microorganisms. The technology may be used with existing glass flask technology, or coupled to a plastic flask that may be configured for either single-use, or multiple-use.

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04-10-2012 дата публикации

Plastic pressure vessel for biopharmaceutical applications and methods thereof

Номер: US20120252118A1
Принадлежит: 3M Innovative Properties Co

Described herein is a molded plastic pressure vessel for biopharmaceutical applications and methods thereof. The molded plastic pressure vessel has a surface area of at least 500 inches2 and comprises a polyphenylene oxide polymer; and at least one antioxidant.

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06-12-2012 дата публикации

System and plant for cultivation of aquatic organisms

Номер: US20120309081A1
Автор: Ra'anan Herzog
Принадлежит: Univerve Ltd

Provided are a cultivating system for cultivating aquatic organisms including a load-bearing structure having a top portion with at least two top rims transverse to each other and defining a top portion, a bottom portion, and flexible tank adapted for receiving therein a growing medium and for cultivating therein aquatic organisms. Further, the tank can include at least two sidewalls extending such that at least in one cross-section taken along a plane perpendicular to said top portion, at least two of the sidewalls form a general V-shape converging towards the bottom portion. The system can also include a gas emitting arrangement linkable to a source of pressurized gas and comprising gas emitting nozzles disposed within the flexible tank at the bottom portion.

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27-12-2012 дата публикации

Method of culturing eukaryotic cells

Номер: US20120329151A1
Принадлежит: F Hoffmann La Roche AG

An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.

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03-01-2013 дата публикации

Thermoresponsive substrate with microgels, method for its preparation and culture method for biological cells

Номер: US20130005039A1

A substrate ( 10 ), in particular for receiving biological cells ( 21 ) comprises a substrate body ( 1 ) which has a support area ( 2 ) on which there are fixed thermoreactive microgels ( 3 ) which comprise particles containing a thermoreactive polymer. Also described are a process for the preparation of the substrate ( 10 ) and a method for culturing biological cells ( 21 ) on the substrate ( 10 ).

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14-02-2013 дата публикации

Vessel with adjustable side drain

Номер: US20130037123A1
Принадлежит: Matthew Kusz, Patrick Dujardin, Steven Vanhamel

An apparatus for processing fluid includes a vessel provided with a sidewall including a passage and at least partially defining an interior compartment. A drain tube positioned in the interior compartment may drain fluid through the passage in the sidewall. The drain tube may be angled, and arranged such that the end in the interior compartment may be adapted for movement as the result of manipulation of a structure, such as a coupler, external to the interior compartment. Accordingly, the drain tube may be positioned along the sidewall at a reference point for movement between a first position for draining fluid above the reference point and a second position for draining fluid below the reference point. An indexing feature may also be provided for incrementally adjusting the position of the drain tube, along with an associated tool to facilitate the adjustment. Related methods are also disclosed.

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07-03-2013 дата публикации

Method and system for the production of cells and cell products and applications thereof

Номер: US20130058907A1
Принадлежит: Biovest International Inc

A cell culture system for the production of cells and cell derived products includes a reusable instrumentation base device incorporating hardware to support cell culture growth. A disposable cultureware module including a cell growth chamber is removably attachable to the instrumentation base device. The base device includes microprocessor control and a pump for circulating cell culture medium through the cell growth chamber. The cultureware module is removably attached to the instrumentation base device. Cells are introduced into the cell growth chamber and a source of medium is fluidly attached to the cultureware module. Operating parameters are programmed into the microprocessor control. The pump is operated to circulate the medium through the cell growth chamber to grow cells or cell products therein. The grown cells or cell products are harvested from the cell growth chamber and the cultureware module is then disposed.

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11-04-2013 дата публикации

DEVICE FOR RAPIDLY DETECTING MICROORGANISMS

Номер: US20130089923A1
Принадлежит: 3M INNOVATIVE PROPERTIES COMPANY

The disclosure includes: a coating composition, comprising a powdered cold-water-soluble gelling agent and surface-modified nanoparticles disposed in the powdered cold-water-soluble gelling agent; a coated film that includes a transparent film coated with the coating composition; and a device for growing microorganisms, including the coated film releasably attached to at least a portion of a body member that includes a self-supporting and water-proof substrate. 1. A coating composition , comprising:(a) a powdered cold-water-soluble gelling agent; and(b) surface-modified nanoparticles disposed in the powdered cold-water-soluble gelling agent in a range from 0.00001 wt. % to about 0.1 wt. % of the coating composition, the surface-modified nanoparticles having an average primary particle diameter of less than about 100 nanometers.2. The coating composition of claim 1 , wherein said surface-modified nanoparticles comprise from 0.0001 wt. % to 0.01 wt. % of the coating composition.36.-. (canceled)7. The coating composition of claim 1 , wherein the surface-modified nanoparticles comprise nanoparticles selected from the group consisting of silica claim 1 , titania claim 1 , alumina claim 1 , zirconia claim 1 , vanadia claim 1 , ceria claim 1 , iron oxide claim 1 , antimony oxide claim 1 , tin oxide claim 1 , calcium phosphate claim 1 , hydroxyapatite claim 1 , aluminum/silica and combinations thereof.8. The coating composition of claim 1 , wherein the surface-modified nanoparticles comprise surface groups selected from the group consisting of hydrophobic groups claim 1 , hydrophilic groups and combinations thereof.9. The coating composition of claim 1 , wherein the surface-modified nanoparticles comprise surface groups derived from an agent selected from the group consisting of silane groups claim 1 , organic acid groups claim 1 , organic base groups and combinations thereof.10. The coating composition of claim 1 , wherein the surface-modified nanoparticles comprise ...

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25-04-2013 дата публикации

Fluid mixing systems with adjustable mixing element

Номер: US20130101982A1
Принадлежит: Hyclone Laboratories LLC

A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.

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25-04-2013 дата публикации

Methods of cell culture for adoptive cell therapy

Номер: US20130102075A1
Принадлежит: Wilson Wolf Manufacturing Corp

An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm 2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm 2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5×10 6 cells/cm 2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25×10 5 cells/cm 2 .

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02-05-2013 дата публикации

Closed Photobioreactor System For Continued Daily In Situ Production Of Ethanol From Genetically Enhanced Photosynthetic Organisms With Means For Separation And Removal Of Ethanol

Номер: US20130109084A1
Принадлежит: Algenol Biofuels Switzerland GmbH

The invention provides a device for growing genetically enhanced aquatic photoautotrophic organisms in a stable culture, causing the organisms to produce ethanol, and then separating, collecting, and removing the ethanol in situ.

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02-05-2013 дата публикации

Closed Photobioreactor System For Continued Daily In Situ Production Of Ethanol From Genetically Enhanced Photosynthetic Organisms With Means For Separation And Removal Of Ethanol

Номер: US20130109085A1
Принадлежит: Algenol Biofuels Switzerland GmbH

The invention provides a device for growing genetically enhanced aquatic photoautotrophic organisms in a stable culture, causing the organisms to produce ethanol, and then separating, collecting, and removing the ethanol in situ.

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09-05-2013 дата публикации

Method of producing sugar

Номер: US20130115660A1
Принадлежит: PROTERRO Inc

Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention.

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27-06-2013 дата публикации

MULTILAYER FILM AND CELL CULTURE CONTAINER

Номер: US20130164831A1
Принадлежит: TOYO SEIKAN KAISHA, LTD.

Provided are a multilayer film having excellent gas permeability and excellent handling properties and hence is suited for forming a cell culture container, and a cell culture container formed by using the same. 1. A multilayer film used for forming a cell culture container , comprising:{'sup': 3', '3, 'a base material composed of a polyethylene-based resin having a density of 0.87 g/cmto 0.90 g/cm; and'}{'sup': 3', '3, 'an inner layer composed of a polyethylene-based resin having a density of 0.896 g/cm0.93 g/cmand forming the cell culture surface'}2. The multilayer film according to which has at least three layers and has an outer layer composed of a polyethylene-based resin having a density of 0.886 g/cmto 0.93 g/cm.3. The multilayer film according to wherein the density of the polyethylene-based film as the base material is 0.87 g/cm to 0.885 g/cm.4. The multilayer film according to claim 1 , wherein the thickness of the base material relative to the thickness of the multilayer film is 60% or more.5. The multilayer film according to claim 1 , wherein the oxygen permeability is 750 ml·mm/m·day·atm or more (37° C.-80% RH).6. The multilayer film according to claim 1 , wherein the carbon dioxide permeability is 2100 ml·mm/m·day·atm or more (37° C.-80% RH) claim 1 ,7. The multilayer film according to any of claim 1 , wherein the inner layer forming the cell culture surface does not comprise an anti-blocking agent and/or a slipping agent and/or a lubricant.8. A cell culture container formed by the multilayer film according to .9. The cell culture container according to claim 8 , wherein the multilayer film forming the container is 150 μm or less. The present invention relates to a cell culture technology, in particular, relates to a multilayer film having excellent gas permeability and a cell culture container.In recent years, in the field of production of medical supplies, gene therapy, regenerative therapy, immune cell therapy or the like, a large amount of a cell, ...

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04-07-2013 дата публикации

Stimuli Responsive Nanofibers

Номер: US20130171331A1
Принадлежит: Individual

A stimuli responsive nanofiber that includes a stimuli responsive polymer, such as a thermally responsive polymer, and a cross-linking agent having at least two latent reactive activatable groups. The nanofiber may also include a biologically active material or a functional polymer. The stimuli responsive nanofiber can be used to modify the surface of a substrate. When the nanofiber includes a thermally responsive polymer, the physical properties of the surface can be controlled by controlling the temperature of the system, thus controlling the ability of the surface to bind to a biologically active material of interest.

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25-07-2013 дата публикации

Automated cell culture system and process

Номер: US20130189723A1
Принадлежит: Global Cell Solutions Llc

The present invention relates generally to the field of cell culture, which is a laboratory process used primarily for the growth, propagation, and production of cells for analysis and the production and harvesting of cell products. The present invention comprises functionalized and/or engineered hydrogel microcarriers that exhibit any or all of the following properties: controllable buoyancy, ferro- or paramagnetism, molecular or fabricated reporting elements, and optical clarity. The microcarriers are used in a bioreactor that employs external forces to control said microcarrier kinetic energy and translational or positional orientation in order to facilitate cell growth and/or cellular analysis. The bioreactor can be part of an automated system that employs any or all of the following; a microcarrier manufacturing method, a monitoring method, a cell culture method, and an analytical method. Either a single bioreactor or a plurality of bioreactors are used in the automated system to enable cell culture and analysis with a minimum of human intervention.

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15-08-2013 дата публикации

Biomaterial handling device

Номер: US20130210123A1
Принадлежит: Jackson Laboratory

A biomaterial handling device is described that will provide for functionality for performing both IVF procedures and washing techniques in a single device. Disclosed embodiments provide increased protection to biomaterial samples during processing and handling. Embodiments of the invention reduce labor intensive processes for both IVF and washing treatments and address reduced risks of contamination of biological samples by providing an increasingly sterile environment.

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19-09-2013 дата публикации

Automated apparatus and method of cell culture

Номер: US20130244322A1
Принадлежит: CellProthera

The invention provides an automated apparatus of cell culture having tanks of culture medium, of growth factors and of cells to be cultured, an incubator having a thermostated enclosure which houses a cell culture vessel, and control computer system. A supporting and agitation device of the culture vessel is provided in the enclosure, and the culture vessel is formed by a bag having at least one inlet port connected to the tanks and one outlet port connected devices for harvesting and storage of the cells after culture, these harvesting and storage devices and tanks being located outside the enclosure and being connected to the cell expansion bag ports by conduits which together with the cell expansion bag form a preassembled module passing through a wall of the enclosure.

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31-10-2013 дата публикации

LINEARLY SCALABLE SINGLE USE BIOREACTOR SYSTEM

Номер: US20130288346A1
Принадлежит: XCellerex Inc.

Disclosed is a single-use bioreactor bag design providing substantially equivalent maximum shear rate at the impeller tip and average or bulk shear rate over a range of power per unit working volume and rpm needed for bioculture processing. The uniformity in shear profile of the bag design providing a unique advantage when used as a scale-down or scale-up platform. Also disclosed is a linearly scalable, single-use, bioreactor system for use in carrying out a scalable biomanufacturing process, the system comprising two single-use bioreactor bags of different volumes, wherein the ratio H/D of the height of the working volume H to the diameter D of the tank, or of the bag is equal to about 1.5, and the bulk shear in each of the bags is substantially constant. The disclosed small scale system models larger scale systems from both a shear/scalability/performance basis and also from a validation and regulatory basis. In summary, the 10 L bioreactor system disclosed provides for “linear scalability” by using the same or substantially simulating the same vessel geometry, gas sparging system, impeller shape and type, polymer composition of the flexible bag/components, process control system, and shear rates as larger 50-5000 L systems. 1. A linearly scalable bioreactor system for use in carrying out a scalable biomanufacturing process , the system comprising:a bioprocessing bag having a flexible wall, a working volume of from about 2 liters to about 10 liters, and a height to diameter ratio at maximum working volume of about 1.5; andan impeller mounted on an impeller plate attached to an inside bottom surface of the flexible wall, the ratio of the diameter of the impeller, Di, to the diameter of the bioprocessing bag, Dt, equal to a value of from about 0.6 to about 0.7, the bioprocessing bag capable of exhibiting substantially the same impeller shear rate and bulk shear rate over a range of values of power per unit volume.2. The linearly scalable bioreactor system of claim 1 ...

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14-11-2013 дата публикации

Polymeric Film for Use in Bioprocessing Applications

Номер: US20130302894A1
Принадлежит: CRYOVAC, INC.

The presently disclosed subject matter is directed to a polymeric films suitable for use in a wide variety of applications, including (but not limited to) the formation of bioprocessing containers. Particularly, the disclosed films comprise a first barrier layer comprising polyglycolic acid, polyamide, EVOH, and/or an EVOH blend and a second barrier layer comprising EVOH. The dual barrier layers of the film maintain a high gas barrier under a variety of relative humidity conditions. In addition, the disclosed film is biologically inert and free from film surface-modifying additives such that the film does not inhibit the growth of biological cell cultures. 2. The film of claim 1 , wherein the olefin hydrocarbon polymer is selected from the group comprising: cyclic olefin copolymer claim 1 , cyclic olefin homopolymer claim 1 , poly(3-methyl-1-butene) claim 1 , poly(4-methyl-1 pentene) claim 1 , poly(3 claim 1 ,3-dimethyl-1-butene) claim 1 , poly(4 claim 1 ,4-dimethyl-1-pentene) claim 1 , poly(vinyl t-butyl ether) claim 1 , polystyrene claim 1 , and combinations thereof.3. The film of claim 1 , wherein the alpha-olefin copolymer is selected from the group comprising: linear medium density polyethylene claim 1 , linear low density polyethylene claim 1 , and very low density polyethylene.4. The film of claim 1 , wherein the sealant layer comprises about 10-30 weight percent olefin hydrocarbon polymer and about 70-90 weight percent alpha olefin copolymer claim 1 , based on the total weight of the sealant layer.5. The film of claim 1 , wherein the first barrier layer comprises a blend of about 1-50 weight percent polyglycolic acid and about 50-99 weight percent polyamide claim 1 , based on the total weight of the layer.6. The film of claim 1 , wherein the first barrier layer comprises about 100% polyglycolic acid.7. The film of claim 1 , wherein the first barrier layer comprises about 100% polyamide claim 1 , based on the total weight of the layer.8. The film of claim 1 , ...

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14-11-2013 дата публикации

Deformable transportable bioreactor chamber

Номер: US20130302897A1
Принадлежит: ILLINOIS TOOL WORKS INC

An apparatus and method is described for seeding and culturing cells on a sample. The apparatus includes a chamber in which the volume of the chamber may be adjusted without compromising the seal or sterility of the chamber. The apparatus enables the seeding of cells in a reduced volume and culturing of cells in an increased volume. Further, the apparatus enables application of forces, strains and torques to a sample during seeding, culturing or transportation of the sample.

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16-01-2014 дата публикации

Method and bioreactor for the cultivation of microorganisms

Номер: US20140017769A1
Принадлежит: Georg Fischer Piping Systems Ltd, LGEM BV

A method for the cultivation of microorganisms, especially phototrophic microorganisms in a bioreactor or photobioreactor, wherein bicarbonate ions and carbonate ions or a cation surplus concentration in the culture medium is added. A tubular bioreactor and photobioreactor is disclosed for carrying out the method.

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06-02-2014 дата публикации

REACTION VESSEL

Номер: US20140038276A1
Принадлежит:

We provide a miniature, disposable reactor vessel for bioprocessing. Embodiments include a sealed vessel surrounding a filter through which spent media may be preferentially removed relative to culture cells. Preferred embodiments include an impeller shaft that is contained within the vessel and passes through the filter assembly. The impeller shaft may be engaged magnetically with a drive shaft. Combinations of these reactor vessels and methods of their use are also described. 1. A reaction vessel comprising: a cylindrical outer wall having a top opening and a bottom opening and defining a shell interior;', 'a vessel top covering the top opening;', 'a drive shaft receptable defined by a second cylindrical wall on the vessel top, opposite the shell interior;', 'an impeller shaft receptacle defined by a third cylindrical wall on the vessel top, extending partially into the shell interior;', 'a securing receptable defined by a fourth cylindrical wall on the vessel top, said fourth cylindrical wall extending partiall into the shell interior to a distance greater than the impeller shaft receptacle extends, said securing receptacle concentric with and having a greater diameter than the impeller shaft receptacle; and', 'a vessel bottom covering the bottom opening., 'a reactor containment shell comprising2. The reaction vessel of claim 1 , said vessel top further comprising a plurality of access ports permitting access to the shell interior from outside the reaction vessel.3. The reaction vessel of claim 2 , wherein at least one of said access ports is connected to a closed cannula extending into the shell interior.4. The reaction vessel of claim 2 , wherein the access ports lead to a least one member of the group consisting of an oxygen sparger claim 2 , an air sparger claim 2 , a nitrogen inlet claim 2 , a carbon dioxide inlet claim 2 , a cell harvest tube claim 2 , a liquid addition inlet claim 2 , a vent claim 2 , an overlay gas inlet claim 2 , a drawoff tube claim 2 , ...

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06-03-2014 дата публикации

Methods for inactivating fluid cultures through heating

Номер: US20140065015A1
Принадлежит: Hyclone Laboratories LLC

A method for inactivating a fluid culture includes inserting a collapsible bag within a chamber of a tank assembly. A fluid is dispensed into a compartment of the collapsible bag, the fluid including a culture containing live cells or microorganisms. A lid is positioned over an opening of the tank assembly so that the collapsible bag is substantially enclosed within the tank assembly. The fluid within the collapsible bag is heated to an inactivation temperature that is sufficiently high to kill all of the live cells or microorganisms within the fluid. In one embodiment, the fluid within the flexible bag is mixed while the fluid is being heated to the inactivation temperature.

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06-03-2014 дата публикации

MULTILAYERED CELL CULTURE APPARATUS

Номер: US20140065709A1
Принадлежит: CORNING INCORPORATED

A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications. 1. A cell growth apparatus comprising:a plurality of cell growth chambers, each having a gas permeable, liquid impermeable membrane, an opposing surface, and at least one side wall connected to at least one of the gas permeable, liquid impermeable membrane and the opposing surface;at least two tracheal spaces, each tracheal space in communication with at least one gas permeable, liquid impermeable membrane of at least one cell growth chamber;wherein the at least two tracheal spaces comprises peripheral supports on a peripheral edge of the tracheal space; wherein the supports on the peripheral edge of the tracheal space are spaced apart to create a plurality of gaps to allow gasses to flow from an external environment into the tracheal space through the plurality of gaps between peripheral supports.2. The cell growth apparatus of wherein the fluid access to the plurality of cell culture chambers is arranged in parallel.3. the cell growth apparatus of wherein the cell growth chambers are arranged in staggered configuration.4. The cell growth apparatus of wherein the fluid access to the plurality of cell culture chambers is arranged in series.5. The cell growth apparatus of further comprising a fluid flow ...

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20-03-2014 дата публикации

REACTOR

Номер: US20140080213A1
Принадлежит:

The invention relates to a reactor driven in an oscillatory-rotary manner about its fixed, preferably vertical, axis, for preferably biotechnological and pharmaceutical applications. By means of its process-intensifying properties for mixing, suspension, gaseous material transport, heat transfer, irradiation and particle retention, the applicability on the industrial scale is ensured. The reactor which succeeds without a shaft seal permits particularly robust production with respect to sterile technique with avoidance of cleaning and cleaning validation required when the reactor is constructed as a single-use reactor. 1. A reactor comprising a reactor vessel removably inserted in and detachably attached to a reactor housing , a drive unit adapted to rotate the reactor housing , with reactor vessel inserted therein , in periodically opposite directions; said reactor housing being inserted into a holder or on an axial bearing , wherein contents of the reactor vessel are rotated in periodically opposite directions , about a fixed vertical , axis of the reactor , wherein a mechanical power input from the drive unit into the reactor vessel contents is enabled by an angular cross section and a flat , pyramidal or tetrahedral base a shell form of the reactor vessel , by mixing elements fixed within the reactor vessel , or by both.24-. (canceled)5. The reactor as claimed in claim 1 , wherein the reactor vessel has a ratio of height to mean diameter of 0.2-2.0.6. The reactor as claimed in claim 1 , wherein said mixing elements have functionalized surfaces for carrying out physical claim 1 , biological claim 1 , biochemical or chemical reactions at or in membranes claim 1 , for gas distribution claim 1 , for liquid distribution claim 1 , for irradiation claim 1 , for filtration claim 1 , for absorption claim 1 , for adsorption claim 1 , for analysis or for cooling or heating.7. The reactor as claimed in claim 1 , wherein said reactor vessel comprises clamping elements claim 1 ...

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10-04-2014 дата публикации

BIOREACTORS APPARATUS, SYSTEM AND METHOD

Номер: US20140099685A1
Принадлежит:

The present invention provides a bioreactor having a reactor chamber and one or more support chambers. The reactor chamber can have one or more flexible walls for enclosing microorganisms and culture medium. The reactor chamber provides an enclosure for microorganism and culture medium. The support chamber can also have one or more flexible walls. When inflated to a predetermined amount, the support chamber causes the microorganisms and culture medium to distribute to a substantially even depth across the reactor chamber. 1. A bioreactor comprising:a reactor chamber with one or more flexible walls for enclosing microorganisms and culture medium; anda support chamber with one or more flexible walls wherein inflating the support chamber to a predetermined amount causes the microorganisms and culture medium to distribute to a substantially even depth across the reactor chamber.2. The bioreactor of claim 1 , wherein the substantially even depth across the reactor chamber averages between about 5 mm to about 30 mm.3. The bioreactor of claim 1 , wherein the substantially even depth across the reactor chamber ranges between depths of about 5 mm to about 30 mm.4. The bioreactor of claim 1 , wherein the predetermined amount causes the microorganisms and culture medium to distribute to a substantially even depth across the reactor chamber in a convex claim 1 , crescent shape.5. The bioreactor of claim 1 , wherein the support chamber is a base chamber positioned underneath the reactor chamber.6. The bioreactor of claim 5 , wherein the base chamber is an abrasion and/or tear resistant material.7. The bioreactor of claim 1 , wherein the support chamber is a cover chamber positioned overtop the reactor chamber.8. The bioreactor of claim 7 , wherein the cover chamber is a UV stable material.9. The bioreactor of claim 7 , wherein the cover chamber is transparent for light of a wavelength that is photosynthetically active to the microorganisms.10. The bioreactor of claim 1 , further ...

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10-04-2014 дата публикации

Enhanced oxygen cell culture platforms

Номер: US20140099717A1
Принадлежит: UNIVERSITY OF MIAMI

Provided is a cell culture apparatus for culturing cells, that provides enhanced oxygen delivery and supply to cells without the need for stirring or sparging. Oxygen diffusion occurs on both sides of the culture vessel, top and bottom. A gas-permeable membrane that includes perfluorocarbons or fluorocarbon derivatives (e.g., fluorinated silane, partially fluorinated silane) in its composition allows for the rapid, enhanced and uniform transfer of oxygen between the environment of cells or tissues contained in the cell culture container apparatus and the atmosphere of the incubator in which the cell culture apparatus is incubated.

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06-01-2022 дата публикации

MICROORGANISM CULTURE APPARATUS AND MICROORGANISM CULTURE METHOD

Номер: US20220002650A1
Принадлежит:

A microorganism culture apparatus includes a three-layer stacked structure having a layered culture unit () that cultures a microorganism, a layered nutrient supply unit () that is arranged on a first surface () of the culture unit () and supplies a nutrient to the culture unit (), and a layered environmental component supply unit () that is arranged on a second surface () and supplies an environmental component to the culture unit (). 1. A microorganism culture apparatus comprising a three-layer stacked structure that includes:a layered culture unit that cultures a microorganism; andat least one of a layered nutrient supply unit that supplies a nutrient to the culture unit and a layered environmental component supply unit that supplies an environmental component to the culture unit, the layered nutrient supply unit and the layered environmental component supply unit being arranged on a first surface and a second surface opposite to the first surface of the culture unit.2. The microorganism culture apparatus according to claim 1 , wherein in the three-layer stacked structure claim 1 , the nutrient supply unit is arranged on the first surface of the culture unit claim 1 , and the environmental component supply unit is arranged on the second surface.3. The microorganism culture apparatus according to claim 1 , wherein in the three-layer stacked structure claim 1 , the nutrient supply unit is arranged on the first surface and the second surface of the culture unit.4. The microorganism culture apparatus according to claim 3 , wherein the nutrient supply unit arranged on the first surface and the nutrient supply unit arranged on the second surface are different from each other in at least one of a type and a concentration of a nutrient to be supplied.5. The microorganism culture apparatus according to claim 1 , wherein in the three-layer stacked structure claim 1 , the environmental component supply unit is arranged on the first surface and the second surface of the ...

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06-01-2022 дата публикации

CLOSED SYSTEM DEVICE AND METHODS FOR GAS PERMEABLE CELL CULTURE PROCESS

Номер: US20220002659A1
Принадлежит:

Novel methods and apparatus are disclosed for cell culture and cell recovery. The methods and apparatus simplify the process of cell separation from media, minimize potential damage to gas permeable devices during fluid handling, and allow closed system automated cell culture and cell recovery from gas permeable devices. 1. A method of removing media and cells from a cell culture device , the method comprising: a gas delivery component,', 'a first fluid detection component that is capable of determining when a fluid moving within a media removal conduit connected to a cell culture device changes from a liquid to a gas, said first fluid detection component capable of sending a signal to a first fluid flow control component that is capable of terminating the fluid flow through the media removal conduit,', 'a second fluid detection component that is capable of determining when the fluid moving within a cell removal conduit that is connected to the cell culture device changes from liquid to gas, said second fluid detection component capable of sending a signal to a second fluid flow control component that is capable of terminating the fluid flow through the cell removal conduit; said cell culture device comprising a gas permeable bottom upon which cells reside, a media removal conduit, a cell removal conduit, and a filter;, 'using a device to reduce a volume of a liquid media in the cell culture device, said device comprising'}increasing the concentration of cells per milliliter of media within said cell culture device by connecting said gas delivery component to said filter,connecting said first fluid detection component to said media removal conduit,connecting said first fluid flow control component to said media removal conduit,initiating gas delivery from said gas delivery component, whereby gas moves into said cell culture device and displaces media from said cell culture device into a media collection vessel connected to said media removal conduit, said first ...

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05-01-2017 дата публикации

GAS SPARGERS AND RELATED CONTAINER SYSTEMS

Номер: US20170001160A1
Принадлежит:

A container system includes a bag comprised of one or more sheets of flexible polymeric material, the bag having an interior surface at least partially bounding a chamber, the chamber being adapted to hold a fluid. A flexible sparging sheet is secured to the interior surface of the bag so that a compartment is formed between the interior surface of the bag and the sparging sheet, at least a portion of the sparging sheet allowing gas to pass therethrough. A tubular port or tube is secured to the bag so that a passage bounded by the tubular port or tube communicates with the compartment. A mixing element is disposed within the chamber of the bag, the mixing element being spaced apart from the flexible sparging sheet. 1. A container system comprising:a bag comprised of one or more sheets of flexible polymeric material, the bag having an interior surface at least partially bounding a chamber, the chamber being adapted to hold a fluid;a flexible sparging sheet secured to the interior surface of the bag so that a compartment is formed between the interior surface of the bag and the sparging sheet, at least a portion of the sparging sheet allowing gas to pass therethrough;a tubular port or tube secured to the bag so that a passage bounded by the tubular port or tube communicates with the compartment; andmeans for mixing fluid within the chamber of the bag, the means for mixing the fluid being separate from the flexible sparging sheet.2. The container system as recited in claim 1 , wherein the means for mixing the fluid is at least partially disposed within the chamber of the bag.3. The container system as recited in claim 1 , wherein the means for mixing the fluid comprises a movable mixing element disposed within the chamber of the bag.4. The container system as recited in claim 1 , wherein the means for mixing the fluid comprises an impeller or vertical mixer disposed within the chamber of the bag.5. The container system as recited in claim 1 , wherein the sparging sheet ...

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07-01-2016 дата публикации

Use of a transparent composition for photobioreactors

Номер: US20160002581A1
Принадлежит: Arkema France SA

The present invention relates to photobioreactors and more particularly to the use of a transparent composition based on at least one methacrylic polymer for constructing installations for the culture of photosensitive organisms. This composition can be in the form of films, plates, profiled elements or cylinders such as tubes. The invention also relates to a transparent multilayer structure comprising at least one layer of a methacrylic polymer and one layer comprising at least one antifouling additive, and to the use thereof for constructing installations for the culture of photosensitive organisms.

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07-01-2016 дата публикации

Structure for culturing cells

Номер: US20160002583A1
Принадлежит: DDNT CONSULTANTS AUSTRALIA Pty Ltd

The present invention relates to a structure and a container for culturing cells. In one arrangement, the structure ( 200 ) comprises a partially enclosed cavity ( 204 ) and a plurality of compartments ( 201 ) adapted for fluid communication with the partially enclosed cavity ( 204 ). Each compartment ( 201 ) is configured to hold one or more cell within its compartment ( 201 ) at a location dependent on a dimension of the cell. This arrangement may facilitate visual identification or indication of the size of the cell by observing its location within its compartment ( 201 ). The structure ( 200 ) may be included or formed integrally with a container ( 100 ) such as a Petri dish.

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07-01-2016 дата публикации

CELL CULTURE DISH SUPPORTING SIMULTANEOUSLY JUXTAPOSED AND SEPARATED CULTURES

Номер: US20160002585A1
Принадлежит:

The present invention relates to a novel culturing apparatus (assembly) which can be termed a cell culture dish. More particularly, the present invention is a “combined cell culture dish” or “dish-in-dish” apparatus comprising at least one smaller cell culture dish fixedly positioned within a larger cell culture dish, and the number of such fixated cell culture dishes can include a multiple number of fixated cell culture dishes within one another, either concentric or eccentric, in any number of geometric shapes, and without limitation to the number of petri dishes included. An alternate embodiment of this invention can include a plurality of cell culture dishes juxtaposed side-by-side having common interior well walls, and the well walls may or may not be different in height depending on the application. 1. A cell or tissue culture assembly , comprising: a common base having formed within it a plurality of wells defined by walls of differing heights for receiving materials:a. said wells formed in said base providing transparent distortion-free access for viewing cells or tissues which are influencing each other;b. said base having a front end and a back end;c. said base having a surround well wall dimensionally higher than all other well walls running around said base;d. said base having one or a plurality of center well walls dimensionally lower than said surround w ell wall;e. said w ells formed in said base each having vertical well walls;f. said surround well wall having a lower edge which is the lowest extremity which is confluent with said base;g. said well walls each having well wall rims or upper edges which constitute the upper extremities of said base and which all lie in a plane that is perpendicular to the plane in which said lower edge of said base lies.2. The cell or tissue culture assembly of claim 1 , further characterized by said wells having interiors which atmospherically communicate with each other as influenced by said surround and center cell ...

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07-01-2016 дата публикации

DEVICE AND METHODS FOR PLATELET LYSIS OR ACTIVATION

Номер: US20160002598A1
Принадлежит: Regenerative Science, LLC

Device, system and method embodiments are disclosed herein which provide for the production of a modified autologous platelet solution at the patient bedside for contemporaneous reinjection to the patient. In certain embodiments all of the steps including, but not limited to blood draw, platelet lysis, solution preparation and reinjection to a patient may be accomplished in a single office or clinic visit without relocating the patient. Accordingly, the apparatus, devices and systems disclosed herein generally include a substantially stand-alone machine, device or system which is configured to accept a platelet containing solution, induce lysis of one or more platelet bodies within the platelet containing solution and provide the resulting modified platelet solution in a manner suitable for injection into the patient. 1. A device comprising:a housing;an input port into the housing providing for the input of a platelet containing solution;a lysis/activation chamber in fluid communication with the input port and positioned within the housing, wherein one or more platelets within the platelet containing solution input to the device at the input port are caused to undergo lysis or activation in the lysis/activation chamber, thereby creating modified solution; andan outlet port from the housing, in fluid communication with the lysis/activation chamber, providing for the modified solution to be removed through the outlet port.2. The device of further comprising a heating and cooling module in thermal communication with the lysis/activation chamber claim 1 , said heating and cooling module providing for the thermal lysis or activation of one or more platelets of the platelet containing solution within the lysis/activation chamber.3. The device of wherein the lysis/activation chamber comprises a length of disposable sterile tubing.4. The device of wherein the heating and cooling module causes the platelet containing solution to wholly or partially freeze and wholly or ...

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05-01-2017 дата публикации

Imaging Apparatus, Imaging System and Incubator

Номер: US20170002307A1
Автор: Kunio Ikefuji
Принадлежит: Screen Holdings Co Ltd

An imaging apparatus comprises: a culture container including an accommodation space for accommodating a sample carrier carrying biological samples in a culture environment for the biological samples and a first transparent part making the accommodation space observable from outside; and an imager that images the biological samples in the accommodation space via the first transparent part.

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04-01-2018 дата публикации

Aseptically Connectable Sensor Patch

Номер: US20180002653A1
Автор: KAISERMAYER CHRISTIAN
Принадлежит:

The invention discloses a first connection unit having a plurality of sensor surfaces and which is adapted to be asepetically connected to a second connection unit mounted e.g. on a flexible bioreactor bag. 1. A first connection unit adapted to mate aseptically with a second connection unit , wherein said first connection unit comprises a plurality of sensor surfaces.2. The first connection unit of claim 1 , comprising a first terminal end surface claim 1 , which is provided with a first opening of a recess claim 1 , wherein said first opening is sealed by at least a first releasably adherent film arranged on said first connection unit claim 1 , so that the contact between the film and the first connection unit is aseptic.3. The first connection unit of claim 2 , wherein said plurality of sensor surfaces are arranged in said recess or on an inner surface of said recess.4. The first connection unit of claim 2 , wherein said at least a first releasably adherent film is arranged to mate with a second releasably adherent film arranged on a second terminal surface of said second connection unit claim 2 , having a second opening claim 2 , and said first and second films are adapted to be pulled out together two and two after mating claim 2 , such that corresponding first and second openings in said first and second terminal end surfaces are mated aseptically.5. The first connection unit of claim 1 , comprising at least two claim 1 , such as at least three or at least four sensor surfaces.6. The first connection unit of claim 1 , comprising at least one claim 1 , such as at least two claim 1 , three or at least four enzymatic sensor surfaces.7. The first connection unit of claim 2 , wherein a ratio between a depth and a width of said recess is less than 1 claim 2 , such as less than 0.5 or less than 0.25.8. The first connection unit of of claim 2 , wherein said plurality of sensors are arranged on a movable member.9. A second connection unit claim 1 , adapted to mate ...

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04-01-2018 дата публикации

SPHEROID CELL CULTURE ARTICLE AND METHODS THEREOF

Номер: US20180002660A1
Принадлежит:

A spheroid cell culture article including: 119-. (canceled)20. A cell culture article comprising: a side wall', 'a top aperture; and', 'a gas-permeable , liquid impermeable bottom comprising at least one concave surface, wherein at least a portion of the bottom is transparent and wherein at least a portion of the bottom comprises a low-adhesion or no-adhesion material in or on the at least one concave surface., 'a chamber comprising21. The article of further comprising a liner having a porous membrane situated within a portion of the chamber to divide the chamber into an upper chamber and a lower chamber.22. The article of further comprising a chamber annex for receiving a pipette tip for aspiration claim 20 , the chamber annex comprises a surface adjacent to and in fluid communication with the chamber claim 20 , the chamber annex having a second bottom spaced away and at an elevation above the gas-permeable claim 20 , transparent bottom claim 20 , wherein the second bottom deflects fluid dispensed from a pipette away from the transparent bottom.23. The article of further comprising a liner having a porous membrane situated within a portion of the chamber to form an upper chamber and a lower chamber the porous membrane providing separation of a second cellular material in the upper chamber claim 22 , from a first cellular material in the lower chamber .24. The article of wherein the article comprises from 1 to about 2 claim 20 ,000 of said chambers claim 20 , wherein each chamber is physically separated from any other chamber.25. The article of wherein the at least one concave surface comprises a plurality of concave surfaces within the same chamber.26. The article of wherein the at least one concave surface comprises a hemispherical surface claim 20 , a conical surface having a taper of 30 to about 60 degrees from the side walls to the bottom claim 20 , or a combination thereof.27. The article of wherein the side wall surface comprises a vertical cylinder claim 20 ...

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04-01-2018 дата публикации

METHOD AND DEVICE FOR CLOSED SYSTEM CULTURE OF CARTILAGE TISSUE

Номер: US20180002667A1
Принадлежит:

The present approach relates to the design and use of a functionally closed bioreactor designed to immobilize, culture, and mature tissue on a loading platform. The bioreactor may be equipped with sensors for tissue monitoring which in conjunction with stiffness data can provide closed-loop control of tissue maturation. Based on a relationship between cartilage stiffness and tissue maturity, measurements of stiffness can be acquired and used as a surrogate for cartilage maturity without the need for destructive tests. 1. A bioreactor system , comprising:a sample support table configured to hold one or more flexible vessels each containing one or more tissue samples;a force measurement sensor positioned so that when a respective flexible vessel is positioned on the sample support table at a measurement location, the respective flexible vessel rests on the force measurement sensor; andan actuator configured to move along an axis to engage and disengage the respective flexible vessel when positioned at the measurement location such that, when engaged, the actuator generates a displacement and corresponding displacement data and the force measurement sensor measures the force and generates force data, wherein the data are used to generate a tissue stiffness measure.2. The bioreactor system of claim 1 , actuator is configured to apply a compressive load to a tissue sample within the flexible vessel during operation.3. The bioreactor system of claim 1 , wherein the sample support table comprises a rotating table configured to move a plurality of tissue samples in succession over the force measurement sensor.4. The bioreactor system of claim 1 , wherein at least one flexible vessel contains a plurality of discrete tissue samples claim 1 , wherein the bioreactor system is capable of separately positioning each discrete tissue sample over the force sensor.5. The bioreactor system of claim 1 , wherein the force measurement sensor comprises at least one load cell.6. The ...

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02-01-2020 дата публикации

CELL OBSERVATION APPARATUS

Номер: US20200002661A1
Принадлежит:

There is provided a cell observation apparatus having a sterile chamber containing a culture vessel and a transparent observation window provided in a bottom portion of the sterile chamber and having the culture vessel placed thereabove, wherein the camera and the lighting are provided below the observation window The cells in the culture vessel are imaged through the observation window by setting the focus of the camera to a bottom surface of the culture vessel The lighting emits inspection light L as spot light where the light is not diffused, and an imaging range of the camera is positioned inside an irradiation range of the inspection light L on the bottom surface of the culture vessel 1. A cell observation apparatus comprising a camera imaging cells contained in a culture vessel and a lighting irradiating the cells with inspection light , characterized by comprising:a sterile chamber, an inside of which is maintained in a sterile condition and which contains the culture vessel; and a transparent observation window provided in a bottom portion of the sterile chamber and having the culture vessel placed thereabove, whereinthe camera and the lighting are provided below the observation window, and the cells in the culture vessel are imaged through the observation window by setting the focus of the camera to a bottom surface of the culture vessel,the lighting emits the inspection light as spot light where the light is not diffused, and an imaging range of the camera is positioned inside an irradiation range of the inspection light on the bottom surface of the culture vessel, andan imaging direction of the camera and an irradiation direction of the lighting are set so as not to be at the same angle relative to an axis orthogonal to the bottom surface of the culture vessel.2. The cell observation apparatus according to claim 1 , characterized by comprisinga moving unit moving the camera and the lighting integrally while maintaining the imaging direction of the camera ...

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02-01-2020 дата публикации

SYSTEM, APPARATUS AND METHOD FOR MATERIAL PREPARATION AND/OR HANDLING

Номер: US20200002663A1
Принадлежит:

Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency. 1. (canceled)2. A lysing apparatus , comprising:a container having at least one chamber to hold a material to be lysed and a lysing particulate material, the chamber having a first opening that provides fluid communication into the chamber from an exterior thereof;an impeller having a number of blades positionable in the chamber of the container; anda micromotor coupled to turn the impeller, at least a portion of the micromotor removably receivable in the first opening of the container to seal the first opening in use.3. The lysing apparatus of wherein the container comprises a second opening positioned above the first opening claim 2 , the second opening provides fluid communication into the chamber from the exterior thereof.4. The lysing apparatus of wherein the container comprises a third opening that provides fluid communication into the chamber from the exterior thereof.5. The lysing apparatus of wherein the first opening is the only opening in the container.6. The lysing apparatus of wherein the micromotor is disposable.7. The lysing apparatus of wherein the micromotor pulsates.8. The lysing apparatus of wherein the micromotor drives the impeller at a rate of greater than 10 claim 2 ,000 RPM.9. The lysing apparatus of wherein the ...

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07-01-2021 дата публикации

Single Use Bioreactor

Номер: US20210002597A1
Принадлежит:

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor. 1131-. (canceled)132. A bioreactor comprising:a bioprocess container made from a liquid impermeable and flexible shape-conforming material, the bioprocess container having a bottom and at least one side wall, the bioprocess container defining a hollow enclosure for receiving a culture media, the bioprocess container including an interior surface configured to contact a culture media contained in the hollow enclosure and an opposite exterior surface, wherein the flexible shape-conforming material comprises a polymer film, the hollow enclosure having a volume of from about 2 L to about 50,000 L;a rigid shell defining an interior volume and having a shape configured to receive the bioprocess container therein, the rigid shell having a bottom portion defining an interior surface, the bioprocess container conforming to the interior surface of the bottom portion of the rigid shell;a mixing device comprising a rotatable shaft coupled to at least one impeller that extends into the hollow ...

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07-01-2021 дата публикации

BIOPROCESSING METHODS FOR CELL THERAPY

Номер: US20210002599A1
Принадлежит: Global Life Sciences Solutions USA LLC

A non-transitory computer readable medium includes instructions configured to adapt a controller to maintain a first target environment in a bioreactor vessel containing a population of cells for a first incubation period to produce a population of genetically modified cells from the population of cells, initiate a flow of media to the bioreactor vessel, maintain a second target environment in the bioreactor vessel for a second incubation period to produce an expanded population of genetically modified cells. 1. A non-transitory computer readable medium comprising instructions configured to adapt a controller to:maintain a first target environment in a bioreactor vessel containing a population of cells for a first incubation period to produce a population of genetically modified cells from the population of cells;initiate a flow of media to the bioreactor vessel; andmaintain a second target environment in the bioreactor vessel for a second incubation period to produce an expanded population of genetically modified cells.2. The non-transitory computer readable medium of claim 1 , wherein:the instructions are configured to adapt the controller to actuate at least one valve to place a reservoir containing the population of cells in fluid communication with the bioreactor vessel, and actuate at least one pump to pump the population of cells from the reservoir to the bioreactor vessel.3. The non-transitory computer readable medium of claim 2 , wherein: the instructions are configured to adapt the controller to actuate at least one valve to place a media bag in fluid communication the reservoir claim 2 , and actuate at least one pump to pump media from the media bag into the reservoir to rinse the reservoir claim 2 , and then transfer rinse media from the reservoir into the bioreactor vessel.4. The non-transitory computer readable medium of claim 1 , wherein:the second target environment includes a temperature within the bioreactor vessel and a carbon dioxide ...

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07-01-2021 дата публикации

ASSESSMENT OF MICRO-ORGANISM PRESENCE

Номер: US20210002688A1
Принадлежит:

The present disclosure relates to approaches for assessing a sample or the presence of microorganisms. The sample, in certain implementations may be assessed for one or both of absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). sample partition device may be employed that partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives. 1. A cell analysis system , comprising:an imaging sub-system configured to automatically acquire successive images; and measuring a fill height of each sample-containing well of a plurality of wells of a well plate, wherein the sample-containing wells contain a sample that is partitioned between the sample-containing wells;', 'determining a focal plane height for each sample-containing well based at least in part on the respective fill height of sample within each sample-containing well;', 'acquiring, using the focal plane height determined for each sample-containing well, successive images of the respective sample-containing wells;', 'determining, using the acquired successive images, a change in a measured optical parameter at each sample-containing well over time relative to a baseline; and', 'generating an assessment of micro-organism presence within the sample based on the determined changes in the measured optical parameter at each sample-containing well over time., 'a processing sub-system configured to control operation of the imaging sub-system and to process data acquired using the imaging sub-system, wherein the imaging sub-system and processing sub-system in cooperation perform acts comprising2. The cell analysis system of claim 1 , wherein the imaging sub-system comprise a digital microscopy system.3. The cell analysis system of claim 1 , wherein measuring the fill height of each sample-containing well comprises: ...

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03-01-2019 дата публикации

METHOD FOR MEASURING A PLURALITY OF PARAMETERS OF STATE OF A FLUID CONTAINED IN A CONTAINER

Номер: US20190002810A1
Принадлежит:

A method is provided for measuring parameters of state of a fluid contained in a container (′) that has a sensor carrier plate with plural sensors (S-S, S′-S′). Each sensor has a sensor head in operative contact with the interior of the container (′) and in operative contact with an external control unit (). The external control unit () receives measurement data generated by the sensor heads of active sensors via their communication link and processes the data. An activation data record (′) is assigned to each sensor (S-S, S′-S′) and is recorded in a manner that is accessible for the external control unit (). The external control unit () first accesses the activation data records (′), then classifies the sensors (S-S, S′-S′) as activatable or nonactivatable sensors, and subsequently activates only those sensors classified as activatable sensors. 110101010011001101010121203050104060710. A method for measuring a plurality of parameters of state of a fluid contained in a container ( , ′) configured for a single use , wherein a sensor carrier plate is secured on the wall of the container ( , ′) in a fluid-tight manner , the carrier plate having plural sensors (S-S , S′-S′) , each of which has a sensor head in active contact with an interior of the container ( , ′) and has a communication link with a data exchange connection to an external control unit () , and wherein the external control unit () receives measurement data generated by the sensor heads of active sensors (S , S; S′ , S′ , S′ , S′ , S′) via their communication link and processes them , the method comprising:{'b': 24', '24', '01', '10', '01', '10', '24', '24', '12', '12', '24', '24', '01', '10', '01', '10', '24', '24', '03', '05', '01', '04', '06', '07', '10, 'associating an activation data record (, ′) with each sensor (S-S, S′-S′); storing the activation data record (, ′) in a storage that is accessible for the external control unit (), using the external control unit () to access the activation data ...

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03-01-2019 дата публикации

CELL CULTURE VESSEL AND SAMPLE CELL FOR OBSERVATION USE

Номер: US20190002812A1
Принадлежит:

The present invention provides a cell culture vessel or a sample cell for observation use that makes it possible to observe three-dimensionally-cultured cells from various angles. The cell culture vessel or the sample cell for observation use according to the present invention is characterized by being equipped with a culture gel in which a cell or cell tissue is embedded and a first vessel which encloses the culture gel, wherein a space in the first vessel is filled with the culture gel; and the first vessel has a light-permeable window made from a hydrogel. 1. A cell culture vessel comprising a culture gel in which a cell or cell tissue is embedded and a first vessel which encloses the culture gel , whereina space in the first vessel is filled with the culture gel, andthe first vessel has a light-permeable window made from a hydrogel.2. The cell culture vessel according to claim 1 , wherein the window comprises an agarose gel claim 1 , a polyacrylamide gel claim 1 , a sodium alginate claim 1 , or a collagen gel.3. The cell culture vessel according to claim 1 , wherein the window has the gel strength of 50 g/cmor higher to 10 claim 1 ,000 g/cmor lower.4. The cell culture vessel according to claim 1 , wherein the first vessel is shaped like a cubic shape or a rectangular parallelepiped claim 1 , and the first vessel has the window at each face.5. The cell culture vessel according to claim 1 , wherein the first vessel has a frame bordering the window.6. The cell culture vessel according to claim 1 , further comprising a second vessel for storing a liquid culture medium claim 1 , wherein the first vessel is placed in the liquid culture medium.7. A sample cell for observation use comprising a culture gel in which a cell or cell tissue is embedded and a first vessel which encloses the culture gel claim 1 , whereina space in the first vessel is filled with the culture gel, andthe first vessel has a light-permeable window made from a hydrogel.8. A cell culture vessel ...

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03-01-2019 дата публикации

Substrates for high-density cell growth and metabolite exchange

Номер: US20190002813A1

A polymer or other substrate optimized for growing cells is described, which takes the form of a micro-thin bag with gas permeable sides. Sides of the bag can be held at a fixed distance from one another with a multitude of tiny micropillars or other spacers extending between them, keeping the bag at a predetermined thickness and preventing the bag from collapsing and the sides from sticking together. In other embodiments, the sides may be held apart by gas pressure alone. A 0.01 μm to 1000 μm parylene or other biocompatible coating over the bag outsides controls the permeability of the bag material and provides a bio-safe area for cell growth. An alternate configuration uses open-cell foam with skins coated with a biocompatible coating. Tubes going into multiple bags can be connected to a manifold that delivers gaseous oxygen or removes carbon dioxide and other waste gases. Multiple bags can be stacked together tightly, with o-ring spacers in between, and housed within a vessel to form a high-surface area, ultra-compact cell growing system. For cells growing on the bags, liquid nutrients can be fed by way of the tube spacers, and oxygen and waste gases permeated through the bag sides and transported within the bags.

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03-01-2019 дата публикации

IMPROVEMENTS IN AND RELATING TO CELL HARVESTING APPARATUS

Номер: US20190002817A1
Автор: Dowling Jim, Harris Dan
Принадлежит: GE HEALTHCARE BIO-SCIENCES CORP.

Disclosed herein is a cell harvesting instrument suitable for concentrating cells from a source suspension of cells and/or washing said cells, the instrument comprising: a housing for accommodating mechanical elements including at least one fluid pump, at least one valve; and a processing kit removably insertable into the housing, said kit including a generally flat frame having or supporting plural sealed fluid paths arranged in a generally flat plane and such that fluids in the paths do not contact said mechanical elements, wherein at least portions of the fluid paths comprise flexible tubes, the outer surfaces of which are manipulateable by the or each fluid pump, to provide fluid flow in one or more of the paths and/or by the or each valve to restrict fluid flow in one or more of the paths. In an embodiment, the kit comprises also a fluid processing reservoir and a filter suitable for separating cells from fluid in said paths. A transfer mechanism for moving and weighing the fluid processing reservoir is disclosed also. 1. A pinch valve mechanism operable to pinch a flexible tube against a reaction surface , the mechanism comprisingat least one pivotable arm including on one side a finger to perform said pinching and on an opposing side a cam profile, anda linearly moveable actuator in use acting on the or each cam profile to move an associated finger.2. The mechanism of claim 1 , wherein the at least one pivotable arm comprises a first arm and a second arm each operable to pinch a different tube claim 1 , and wherein the first and second arms share the same actuator.3. The mechanism of claim 2 , wherein the cam profiles of the first and second arms are the same or similar in profile but opposed in orientation such that when the finger of the first arm provides a pinching action the finger of the second arm provides no pinching action claim 2 , and vice versa.4. The mechanism of claim 3 , wherein the opposed cam profiles of the first and second arms have a ...

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13-01-2022 дата публикации

BIOLOGICAL FLUIDIC SYSTEM

Номер: US20220010259A1

Provided is a biological fluidic system that provides a high degree of sterilization by performing the biological processing in a closed system in which the fluid is contained in chambers that are isolated from the environment in a manner that does not permit ingress of contaminants. 1. A system for cultivating microorganisms or performing biological processing , comprising:a fluid driving mechanism; and a processing chamber,', 'a duct extending between two ports of the chamber to permit flow of fluid between the two ports through the duct, and', 'a flow-driving region defined within the duct, flow-driving region is configured for engagement with the fluid driving mechanism to thereby propel fluid flow between the two ports in response to selective pressure on different pliable regions of the flow-driving region., 'a processing arrangement formed between two pliable polymeric sheets, the processing arrangement comprising2. The system of claim 1 , wherein the flow-driving region is generally circular and the fluid driving mechanism is configured to peristaltically propel the fluid.3. The system of claim 1 , wherein the driving mechanism comprises a driving motor with a magnetic element configured for magnetic coupling with an engaging element that is engaged with and configured to propel fluid through the flow-driving region.4. The system claim 1 , comprising a venting arrangement permitting gas exchange between the interior of the processing arrangement and the exterior.5. The system of claim 4 , wherein the venting arrangement is configured to permit gas exchange without passage of microorganism therethrough.6. The system of claim 4 , wherein the venting arrangement comprises a filter for filtering solid particles.7. The system of claim 1 , comprising an inoculation port for introducing living matter into the chamber.8. The system of claim 1 , wherein opposite walls of the processing chamber are fixed to one another in one or more regions other than the edges of ...

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14-01-2016 дата публикации

CONTROLLED GROWTH ENVIRONMENTS FOR ALGAE CULTIVATION

Номер: US20160007549A1
Автор: Oyler James R.
Принадлежит:

A method for cultivating algae can include providing a body of water in a substantially enclosed system. The enclosed system can have a length of channel and a cover. The method can optionally include circulating the body of water through the enclosed system under positive pressure conditions. The positive pressure should prevent ingress of any external atmosphere or material. Further, the method can include cultivating the algae in the body of water at conditions which promote growth. Likewise, a system for cultivating algae can include a channel with a cover, water in the channel, and a pump to introduce positive pressure into the system. 1. A system for cultivating algae , comprising:a channel with a cover system that substantially closes the channel to an outside environment and forms a protected growth volume, the cover system being designed to selectively adjust amounts of light allowed to enter the protected growth volume;a body of water including one or more algae contained in the channel; anda gas pump configured to introduce a positive pressure into the protected growth volume.2. The system of claim 1 , wherein the cover system includes a first cover and a second cover claim 1 , such that the first cover has a greater translucence than the second cover.3. The system of claim 2 , wherein the first cover and the second cover are configured to cover different portions of the channel.4. The system of claim 2 , wherein the second cover is configured to removably cover at least a portion of the channel and the first cover.5. The system of claim 2 , wherein at least a portion of the second cover is opaque or substantially opaque sufficient to block light to promote heterotrophic growth of the algae.6. The system of claim 2 , wherein at least one of the first and second covers includes perforations.7. The system of claim 1 , further comprising a reflecting layer on a portion of an inner wall of the channel within the protected growth volume claim 1 , said ...

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12-01-2017 дата публикации

DISPOSABLE VALVE AND FLEXIBLE CONTAINERS FOR PRESSURIZED BIOREACTORS

Номер: US20170008752A1
Принадлежит: GE HEALTHCARE BIO-SCIENCES CORP.

Disclosed herein are embodiments of a valve assembly for preventing dead leg spaces in a container or tubing, the embodiments including a three-way valve system for controlling back-pressure in a fluid generating device, such as a single-use high pressure bioreactor. Also disclosed is a pressurized reactor system for bioprocessing, comprising a single-use container including a flexible wall or a semi-rigid wall. 2. A three-way valve assembly for controlling back-pressure in a fluid generating device , the three-way valve assembly comprising:a main valve body portion adapted at one end to engage an inlet for exhaust fluid from the fluid generating device, the main valve body portion defining a central axis;a bellows or valve stem having a first end and a second end, comprising a solid plunger at the first end and disposed axially within the main valve body portion, the bellows or valve stem supported for reciprocating axial movement between a first position wherein the solid plunger closes the inlet for exhaust fluid from the fluid generating device and a second position wherein the solid plunger opens the inlet for exhaust fluid from the fluid generating device;a two-way valve coupled to the second end of the bellows or valve stem, the two-way valve configured to selectively control a gas inlet for inflating the bellows and a gas outlet for deflating the bellows.3. The three-way valve assembly of claim 2 , configured in a manner to move the bellows to the first position wherein the solid plunger closes the inlet for exhaust fluid when the bellows are inflated claim 2 , and to move the bellows to the second position wherein the plunger opens the inlet for exhaust fluid when the bellows are deflated.4. The three-way valve assembly of claim 2 , further comprising a tubular body disposed axially within the main valve body portion and configured to retain the bellows or valve stem and direct motion thereof axially.5. The three-way valve assembly of claim 2 , wherein the ...

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12-01-2017 дата публикации

Vertical wheel bioreactors

Номер: US20170009193A1
Автор: J. Gregory Zeikus
Принадлежит: PBS Biotech Inc

A pneumatic bioreactor includes a vessel containing a fluid to be mixed and at least one mixing device driven by gas pressure. A first embodiment includes a floating impeller that rises and falls in the fluid as gas bubbles carry it upward to the surface where the gas is then vented, permitting the impeller to sink in the fluid. The floating impeller may be tethered to a second impeller with a flexible member and pulley. The mixing speed is controlled with electromagnets in the vessel acting upon magnetic material in the impeller or its guides. In another embodiment, floating pistons mix the fluid, pushing it through a mixing plate with one or more apertures. In a third embodiment, the mixing device is a rotating drum with bubble-catching blades and rotating mixing plates with apertures. The top of the vessel for these mixers may include a closed top and sterile filters.

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14-01-2021 дата публикации

Disposable Bioreactor Systems and Related Methods

Номер: US20210008512A1
Принадлежит:

A method for enhancing mixing and aeration of a liquid reaction medium in a toroidal bioreactor vessel includes dispensing a liquid reaction medium into an interior of a toroidal bioreactor vessel, the interior being bounded by an inner surface, a textured surface being arranged on at least a portion of the inner surface, the textured surface having a plurality of upstanding protuberances. The toroidal bioreactor vessel is rotated in an orbital motion such that there is a resonant frequency traveling wave of the fluid orbiting in one direction in the interior of the toroidal bioreactor vessel when a particular orbital speed is imparted to the toroidal bioreactor vessel. 1. An apparatus comprising:(a) a hollow toroidal bioreactor vessel having an interior bounded by an inner surface, a textured surface being arranged on at least a portion of the inner surface, the textured surface having a sinusoidal shape pattern and being comprised of a plurality of upstanding protuberances; and(b) a drive including a motor driven platform, wherein when the toroidal bioreactor vessel rests on the motor driven platform and a fluid is disposed within the interior of the toroidal bioreactor vessel, operation of the drive moves the platform orbitally such that there is a resonant frequency traveling wave of the fluid orbiting in one direction in the interior of the toroidal bioreactor vessel when a particular orbital speed is imparted to the toroidal bioreactor vessel, andwherein the orbiting direction of the traveling wave of the fluid is parallel to a direction of periodic oscillation of the sinusoidal shape pattern of the textured surface.2. The apparatus in accordance with claim 1 , wherein the textured surface is located on a bottom of the toroidal bioreactor vessel.3. The apparatus in accordance with claim 1 , wherein the textured surface is located on a side-wall of the toroidal bioreactor vessel.4. The apparatus in accordance with claim 1 , wherein the textured surface is ...

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08-01-2015 дата публикации

Polysiloxane Substrates with Highly-Tunable Elastic Modulus

Номер: US20150010919A1
Принадлежит:

A highly tunable bioscaffold is provided, as well as a method of manufacture of the bioscaffold and methods of use of the bioscaffold, for example for drug testing, cell propagation and for optimizing growth of a cell type, for example corneal endothelial cells. 1. A sterile bioscaffold comprising a crosslinked mixture of a first composition comprising a polysiloxane and nanoparticles and a second composition comprising independently a polysiloxane and which does not comprise nanoparticles.2. The bioscaffold of in which the first composition further comprises polydimethyl siloxane claim 1 , one or more siloxanes other than polydimethyl siloxane claim 1 , and silica nanoparticles; and the second composition comprises one or both of a polydimethylsiloxane and a dimethyl claim 1 , methylhydrogen siloxane.3. The bioscaffold of claim 1 , in which the polysiloxane of the first and second compositions independently comprise one or more of a dimethyl siloxane; a diphenylsiloxane; a diethylsiloxane; a trifluoropropyl methyl siloxane; a phenylmethylsiloxane; a copolymer of dimethylsiloxane with one or more of a diphenylsiloxane claim 1 , a diethylsiloxane claim 1 , a trifluoropropyl methyl siloxane claim 1 , and/or a phenylmethylsiloxane; and a aminopropylmethylsiloxane-(dimethylsiloxane).4. The bioscaffold of claim 1 , in which the nanoparticles are silica nanoparticles.5. The bioscaffold of claim 1 , in which the nanoparticles comprise organically-modified silica.6. The bioscaffold of claim 5 , in which the organically-modified silica comprises one or more vinyl and/or alkyl groups.7. The bioscaffold of claim 5 , in which the organically-modified silica comprises one or both of dimethylvinylated silica and trimethylated silica.8. The bioscaffold of claim 1 , in which the polysiloxane of one or both of the first composition and the second composition is dimethylvinyl-terminated.9. The bioscaffold of in which the first composition comprises dimethylvinyl-terminated dimethyl ...

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09-01-2020 дата публикации

SYSTEM AND METHOD FOR SELECTING AND CULTURING CELLS

Номер: US20200009309A1
Принадлежит:

A cell processing system includes at least one processor connectable to a source container filled with a biological fluid, the processor including a spinning membrane configured to receive and separate target cells from the biological fluid, the target cells exiting at a first outlet, first and second containers selectively connected to the first outlet; and a magnet. The system also includes a controller configured to operate the spinning membrane to receive biological fluid and to direct the target cells to the first container, to pause to permit magnetic particles to be associated with the target cells in the first container, to operate the spinning membrane to receive the contents of the first container with the magnet applied to the target cells associated with the magnetic particles, to remove or deactivate the magnet, and to transfer the target cells to the second container. 1. A cell processing system comprising: a spinning membrane configured to receive and separate target cells from the biological fluid, the target cells exiting at a first outlet;', 'at least a first container and a second container selectively connected to the first outlet; and', 'a magnet; and, 'at least one processor connectable to a source container filled with a biological fluid, the at least one processor comprising to operate the spinning membrane to receive biological fluid from the source container and to direct the target cells to the first container;', 'to pause operation of the processor to permit magnetic particles to be associated with the target cells in the first container;', 'to operate the spinning membrane to receive the contents of the first container with the magnet applied to the target cells associated with the magnetic particles in the first container;', 'to remove and/or deactivate the magnet applied to the target cells associated with the magnetic particles in the first container;', 'to transfer the target cells to the second container after removal and/or ...

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14-01-2016 дата публикации

PHOTOBIOREACTOR SYSTEMS AND METHODS FOR PRODUCING BIOMASS

Номер: US20160010044A1
Автор: Wu Xiaoxi
Принадлежит:

Certain embodiments and aspects relate to a photobioreactor including encapsulating photobioreactor units (EPU) within which a liquid medium and a gas are encapsulated. The liquid medium comprises at least one species of phototrophic or mixotrophic organism therein. The gas comprises a high concentration of carbon dioxide. Certain methods of using the EPUs for algal biomass production use automated function units. 1. A system for cultivating algae , the system comprising:a plurality of encapsulating photobioreactor units (EPUs) configured to float in a body of water, each EPU comprising a first portion fluidically connected to a second portion; wherein the first portion is configured to hold a liquid media, and the second portion is configured to hold a gas;a handler configured to receive each EPU, harvest at least a portion of the liquid media from each EPU, and add liquid media to each EPU.2. The system of claim 1 , wherein the handler is configured to clean and/or disinfect each EPU.3. The system of claim 1 , further comprising a mixer.4. The system of claim 1 , further comprising a driver.5. The system of claim 1 , further comprising the body of water.6. The system of claim 1 , wherein the first portion of each EPU is immersed in the body of water and the second portion of each EPU is positioned above the body of water.7. The system of claim 5 , wherein the body of water is a natural pond or an artificial pond.8. The system of claim 1 , wherein each EPU comprises at least one sealable port through which contents can be added to and removed from the EPU.9. The system of claim 1 , wherein each EPU comprises transparent or translucent material.10. The system of claim 1 , wherein each EPU comprises a rigid material.11. The system of claim 1 , further comprising the liquid media.12. The system of claim 11 , wherein the liquid media comprises a phototrophic or mixotrophic organism.13. The system of claim 12 , wherein the organism comprises a strain of algae or ...

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11-01-2018 дата публикации

CULTURE APPARATUS

Номер: US20180010081A1
Принадлежит:

A culture apparatus includes: an inner case having an opening in a front surface and stainless-steel shelf rests on left and right side surfaces, the inner case configured to be sterilized with hydrogen peroxide or by dry heat sterilization; and a stainless-steel shelf plate placed on the shelf rests and slid in a front-back direction, the stainless-steel shelf plate configured to be sterilized with hydrogen peroxide or by dry heat sterilization. The shelf plate has a base surface on which a culture is placed, flanges respectively formed by being bent upward, at bent portions in end portions on left and right sides of the base surface, and sliding members respectively provided to outer surfaces of the flanges, at least on a front side in a direction of movement when the shelf plate is being inserted, the sliding members made of a material different from a material of the inner case. 1. A culture apparatus comprising:an inner case having an opening in a front surface and stainless-steel shelf rests on left and right side surfaces, the inner case configured to be sterilized with hydrogen peroxide or by dry heat sterilization; anda stainless-steel shelf plate to be placed on the shelf rests and slid in a front-back direction, the stainless-steel shelf plate configured to be sterilized with hydrogen peroxide or by dry heat sterilization, a base surface on which a culture is to be placed,', 'flanges respectively formed by being bent upward, at bent portions in end portions on left and right sides of the base surface, and', 'sliding members respectively provided to outer surfaces of the flanges on the left and right sides, at least on a front side in a direction of movement when the shelf plate is being inserted, the sliding members made of a material different from a material of the inner case,, 'the shelf plate having'}the sliding members each being a sheet or a film attached to a lower surface of the base surface and each side surface, each of the outer surfaces, of ...

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11-01-2018 дата публикации

Bioreactor With Higher Agitation Rates

Номер: US20180010082A1
Принадлежит:

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor. 1. A bioreactor comprising:a bioprocess container having a top, a bottom, and at least one side wall therebetween, the bioprocess container defining a hollow enclosure for receiving a culture medium;a mixing device comprising a rotatable shaft coupled to at least one impeller; anda motor coupled to the rotatable shaft for rotating the shaft and the at least one impeller, the motor being configured to rotate the impeller at a rate of from 80 rpm to 200 rpm.2. A bioreactor as defined in claim 1 , wherein the mixing device comprises at least one top impeller and at least one bottom impeller that extend into the hollow enclosure of the bioprocess container.3. A bioreactor as defined in claim 2 , wherein the top impeller and the bottom impeller are made from a polymer material claim 2 , the top impeller and the bottom impeller having a hydrophilic surface.4. A bioreactor as defined in claim 1 , further comprising at least one baffle being configured to extend adjacent to the side wall of the ...

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11-01-2018 дата публикации

PREPARING ANTIGEN-SPECIFIC T CELLS USING A SELF-ENCLOSED PROCESSING SYSTEM THAT CONTAINS BOTH A CENTRIFUGE AND A MAGNETIC SEPARATION COLUMN

Номер: US20180010087A1
Принадлежит: Miltenyi Biotec GmbH

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (), a pump () and a plurality of valves (-) configured to at least partially control fluid flow through a fluid circuitry and a separation column () positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column. 1. A method of enriching a defined population of target cells from a mixed population of blood cells using an apparatus that is configured for processing such cells using a sterile tubing set;wherein the tubing set comprises:(a) a first connecting member configured to receive and deliver the sample to a rotatable container,(b) said rotatable container, which is configured to rotate about an axis and thereby to apply centrifugal force to a sample contained in a processing chamber in the container so as to separate cells from the sample from other components of the sample;(c) a second connecting member configured to transfer cells processed by the rotatable container to a cell separation column;(d) said separation column, which is configured to separate labeled cells from unlabeled cells; and(e) a third connecting member configured to transfer cells separated by the cell separation column to a product collection container;wherein the method comprises:(1) delivering the sample through the first connecting member into the tubing set; and(2) operating the apparatus such that the sample is ...

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14-01-2021 дата публикации

METHODS AND MATERIALS FOR MAINTAINING AND EXPANDING STEM CELL-DERIVED HEPATOCYTE-LIKE CELLS

Номер: US20210009947A1
Принадлежит:

Culture medium formulations are described that are capable of large-scale expansion of stem cell-derived hepatocyte-like cells while sustaining the activity of those cells to maintain the phenotype and biological characteristics of normal hepatocytes. 1. A composition comprising a culture medium , said medium comprising hydrocortisone , bovine serum albumin , insulin , transferrin , selenium , epithelial growth factor , basic fibroblast growth factor , fibroblast growth factor 4 , hepatocyte growth factor , stem cell factor , oncostatin M , bone morphogenic protein 4 , and interleukin 1 beta.2. The composition of claim 1 , wherein said culture medium is effective for inducing differentiation of human fetal blood multi-lineage progenitor cells (MLPC) or a clonal line of human fetal blood MLPC to cells having a hepatocyte phenotype3. The composition of claim 1 , wherein said culture medium is effective in the long-term growth and maintenance of primate embryonic stem cell-derived hepatocyte-like cells.4. The composition of claim 1 , said culture medium further comprising an antibiotic.5. The composition of claim 1 , said composition further comprising at least one antibody claim 1 , said at least one antibody having binding affinity for alkaline phosphatase claim 1 , alpha fetoprotein claim 1 , albumin claim 1 , c-reactive protein claim 1 , hepatocyte growth factor receptor claim 1 , complement factor VII claim 1 , complement factor IX claim 1 , nestin claim 1 , SOX-17 claim 1 , P450 CYP 1A2 claim 1 , P450 CYP 3A4 claim 1 , asialo-glycoprotein receptor 1 claim 1 , hepatocyte nuclear factor-4 claim 1 , GATA-4 claim 1 , or alpha-1-antitrypsin.6. A method of producing a population of cells having a hepatocyte phenotype claim 1 , said method comprising:a) providing a collagen-coated culturing device housing a purified population of MLPC or a clonal line of MLPC;b) culturing said purified population of MLPC or said clonal line of MLPC with a differentiation medium ...

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10-01-2019 дата публикации

Flexible Bioprocessing Container with Partial Dividing Partition

Номер: US20190010435A1
Принадлежит:

Methods of separating magnetic particles from a fluid include introducing a fluid mixture that includes a liquid media, a biological component, and magnetic particles into an internal compartment of a container through an inlet, allowing at least a portion of the mixture to travel around a partition formed within the internal compartment and then exit the internal compartment through an outlet on the opposite side of the partition, and applying a magnetic field to the mixture in the internal compartment so that the magnetic particles are retained within the container or internal compartment thereof by the magnetic field. The partition is formed by permanently or reversibly securing upper and lower container walls together, such as by welding or pressing, between the inlet and the outlet such that the media and biological component must flow around the partition and through the magnetic field to exit the internal compartment through the outlet. 1. A method of separating magnetic particles from a fluid , the method comprising:introducing a fluid mixture into an internal compartment of a flexible container through an inlet of the container, the fluid mixture comprising a liquid media, a biological component, and magnetic particles;allowing at least a portion of the fluid mixture to travel around a partition formed within the internal compartment and then exit the internal compartment through an outlet of the container; andapplying a magnetic field to the fluid mixture in the internal compartment so that the magnetic particles become retained within the container by the magnetic field as the media and the biological component flow from the inlet, around the partition and to the outlet.2. The method of claim 1 , wherein the biological component comprises cells.3. The method of claim 2 , wherein the cells comprise activated and/or expanded human T-cells claim 2 , the magnetic particles comprising inert claim 2 , superparamagnetic beads having anti-CD3 and/or anti-CD28 ...

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10-01-2019 дата публикации

ROTATING CULTURE DEVICE AND CULTURE VESSEL FOR USE IN ROTATING CULTURE DEVICE

Номер: US20190010436A1
Принадлежит: JTEC Corporation

The rotating culture device includes a tubular culture vessel long in an axial direction, a support mechanism which supports the culture vessel so that the culture vessel is rotatable about an axis thereof, rotation drive section which rotationally drives the culture vessel, tilting section which tilts the support mechanism so that the axis of the culture vessel is tilted in a vertical direction, and control section which controls operation of the rotation drive section and operation of the tilting section. 1. A rotating culture device comprising:a tubular culture vessel which is long in an axial direction;a support mechanism which supports the culture vessel so that the culture vessel is rotatable about an axis thereof;a rotation drive section which rotationally drives the culture vessel;a tilting section which tilts the support mechanism so that the axis of the culture vessel is tilted in a vertical direction; anda control section which controls operation of the rotation drive section and operation of the tilting section, whereinthe culture vessel has a peripheral wall portion whose shape is cylindrical or a polygonal shape having eight or more sides so that a liquid culture medium in the culture vessel is prevented from being stirred to damage cells, andthe control section controls the tilting section in accordance with a distribution state of cells so that uneven floating positions of the cells in the axial direction are dispersed in the axial direction.2. A rotating culture device comprising:a tubular culture vessel which is long in an axial direction;a support mechanism which supports the culture vessel at both end sides thereof so that the culture vessel is rotatable about an axis thereof;a rotation drive section which rotationally drives the culture vessel;a reciprocating section which reciprocates the support mechanism in a horizontal direction along the axis of the culture vessel; anda control section which controls operation of the rotation drive section ...

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10-01-2019 дата публикации

CELL CULTURE APPARATUS AND METHOD

Номер: US20190010437A1
Принадлежит:

Provided are cell culture apparatus and method. The cell culture apparatus comprises a culture column and a perfusion system, the culture column comprising a first and a second tube-connecting caps and a support for cells to attach and grow thereon. The first and the second tube-connecting caps are respectively provided with a perfusion inlet and a perfusion outlet; The perfusion system comprises a culture medium storage device, a perfusion tube and a perfusion pump. A complete and closed culture column is formed in the cell culture device after the cells are inoculated, and the culturing is completed by the perfusion system to avoid contamination. 1. A cell culture apparatus comprising:a culture column including a first tube-connecting cover, a second tube-connecting cover and at least a scaffold for adherent growth of cells, the first tube-connecting cover, the at least a scaffold and the second tube-connecting cover being sequentially stacked to form a culture column having a closed cavity, and the first tube-connecting cover and the second tube-connecting cover each having a perfusion inlet and a perfusion outlet; anda perfusion system including a culture medium storage apparatus, a perfusion tube and a perfusion pump for powering perfusion, the culture medium storage apparatus, the perfusion pump and the perfusion inlet being sequentially communicated via the perfusion tube.2. The cell culture apparatus according to claim 1 , wherein at least two scaffolds are provided claim 1 , and/or at least two culture columns are provided.3. The cell culture apparatus according to claim 1 , wherein the culture column further includes a sealing ring and a buckle ring having a same number as that of the scaffold claim 1 , the sealing ring being annular and arranged around an edge of the scaffold claim 1 , the buckle ring being annular and arranged around an edge of the sealing ring claim 1 , wherein a buckle ring boss is provided in an inner circumferential side surface of ...

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10-01-2019 дата публикации

CELL AND TISSUE CULTURE CONTAINER

Номер: US20190010439A1
Автор: Blahut Eric
Принадлежит: HISTOGENICS CORPORATION

The invention generally relates to containers for cell and tissue culturing with multiple compartments in fluid communication with each other to provide a common culture environment in each of the compartments while maintaining physical separation of cells and tissue therein. The invention further relates to culture containers providing a sterile culture environment with detachably coupleable lids and open access to each compartment within a container. 1. A method for culturing a tissue comprising:providing a container comprising a bottom wall coupled to a bottom edge of at least one side wall, and an at least one interior wall coupled to the at least one side wall to define at least a first compartment and a second compartment within the container, wherein the at least one interior wall comprises a bottom edge that does not contact the bottom wall, leaving a gap between the bottom wall and the at least one interior wall, wherein the gap allows fluid to pass between the first and second compartments without allowing cells, tissues, or 3D scaffolds to pass therebetween;providing a lid comprising a gas-permeable membrane, wherein the lid is configured to be detachably coupled to a top edge of the at least one side wall;introducing a first living cell of a first cell type to the first compartment and a second living cell of a second cell type to the second compartment in the presence of a cell culture medium;exposing the container to conditions promoting cell growth to produce a first tissue from the first living cell and a second tissue from the second living cell.2. The method of claim 1 , wherein the first cell type or the second cell type is a chondrocyte claim 1 , mesenchymal stem cell claim 1 , fibroblast claim 1 , osteocyte claim 1 , osteoblast claim 1 , synoviocyte claim 1 , induced pluripotent stem cell (iPSC) claim 1 , embryonic stem cell (ESC) claim 1 , lymphocyte claim 1 , adipocyte claim 1 , myofibroblast claim 1 , hepatocyte claim 1 , islet cell claim 1 , ...

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03-02-2022 дата публикации

Robotic method for coating a multiwell plate by a polyelectrolyte multilayer film

Номер: US20220032289A1

The invention concerns a robotic method for coating the bottom surface of at least one well of a multiwell plate by a polyelectrolyte multilayer film, the multiwell plate obtainable according to the method and the use thereof for cell culture.

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03-02-2022 дата публикации

Multicompartment bag for cultivation of cells

Номер: US20220033748A1
Принадлежит: Global Life Sciences Solutions USA LLC

The invention discloses a flexible plastic bag for cultivation of cells, including a top wall film and a bottom wall film, each having an inside and an outside, sealed to each other inside-to-inside by durable weld seams, optionally via one or more side wall films, to form a bag with an inner volume delimited by the durable weld seams; one or more ports through the top and/or bottom wall film for introduction and withdrawal of fluids; one or more frangible weld seams joining the insides of said top and bottom wall films, dividing the bag inner volume into a plurality of cultivation compartments; and one or more gripping means affixed to the top and bottom wall films, adjacent each of the frangible weld seams and adapted to break a specific frangible weld seam by pulling apart the gripping means on the top and bottom wall films.

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03-02-2022 дата публикации

Cell culture chip

Номер: US20220033749A1
Автор: Makoto Yamanaka
Принадлежит: Ushio Denki KK

A cell culture chip from which a liquid retained in an opening is able to be sucked by a simple operation procedure while a liquid in a channel remains is provided.A cell culture chip according to the present invention includes a bottom portion; a base portion formed on an upper surface of the bottom portion; a first well provided by opening the base portion in a first direction extending from a portion of a main surface of the base portion toward the bottom portion and having a shape such that a capillary force thereof is smaller than that of the chamber; a second well provided by opening the base portion in the first direction at a position separated from the first well in a second direction parallel to the main surface; and a tubular chamber that is defined by a region sandwiched between the bottom portion and the base portion and that provides communication between the first and second wells in the second direction. The first well has a shape with which a capillary force of the first well is smaller than a capillary force of the chamber.

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03-02-2022 дата публикации

Device for Distributing a Flow

Номер: US20220033750A1
Принадлежит:

A flow distribution device for bioprocess systems, comprising: a flow distribution manifold () comprising: at least four fluid connection conduits (), wherein each fluid connection conduit () comprises a first end () for fluid connection and an opposite second end (), and wherein at least three of the fluid connection conduits comprise a membrane () and a valve seat (), which membrane () can be put in at least two different positions in relation to the valve seat () for allowing or preventing fluid flow between the first end () and the second end () of the fluid connection conduit (); and a central common compartment () to which the second ends () of each of the fluid connection conduits () are connected, whereby the first ends () of each of the fluid connection conduits () can be in fluid communication with the central common compartment () and wherein the fluid connection conduits () are entering the central common compartment () from at least three different directions; wherein said flow distribution device () further comprises at least three membrane actuation members () which are provided in connection with one membrane () of the flow distribution manifold () each, wherein each of said membrane actuation members () is configured for actuating the membrane () to be in at least two different positions in relation to the valve seat (), wherein a first position of the membrane () allows flow between the first end () and the second end () of the fluid connection conduit () and a second position of the membrane () prevents flow between the first end () and the second end () of the fluid connection conduit (). 2. The flow distribution device according to claim 1 , wherein at least five or at least six fluid connection conduits are provided in the flow distribution manifold.3. The flow distribution device according to claim 1 , wherein the fluid connection conduits are entering the central common compartment from at least four or at least five different directions.4. ...

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03-02-2022 дата публикации

System for Oxygenating a Biological Culture

Номер: US20220033753A1
Принадлежит:

A system for oxygenating a biological culture includes a container bounding a compartment and having a top wall, a bottom wall, and an encircling sidewall extending therebetween; a tubular member projecting into the compartment of the container and terminating at a terminal end; a gas supply coupled with the tubular member and being configured to blow gas through the tubular member; and a mixing element disposed within compartment of the container at a location between the terminal end of the tubular member and the bottom wall of the container, the mixing element being configured to mix the liquid. 1. A system for oxygenating a biological culture comprising:a) a container bounding a compartment and having a top wall, a bottom wall, and an encircling sidewall extending therebetween, the compartment having an upper end disposed toward to the top wall and an opposing lower end disposed toward the bottom wall;b) a tubular member projecting into the compartment of the container and terminating at a terminal end;c) a gas supply coupled with the tubular member and being configured to blow gas through the tubular member; andd) a mixing element disposed within compartment of the container at a location between the terminal end of the tubular member and the bottom wall of the container, the mixing element being configured to mix the liquid.2. The system as recited in claim 1 , wherein terminal end of the tubular member is disposed within the upper end of the compartment and the mixing element is disposed within the lower end of the compartment.3. The system as recited in claim 1 , wherein terminal end of the tubular member is spaced apart from the mixing element.4. The system as recited in claim 1 , wherein the tubular member passes through the sidewall of the container.5. The system as recited in claim 1 , wherein the tubular member passes through the top wall of the container.6. The system as recited in claim 1 , wherein the mixing element comprises an impeller.7. The ...

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03-02-2022 дата публикации

CONTINUOUS RECOVERY HARVEST BAG

Номер: US20220033755A1
Принадлежит: Global Life Sciences Solutions USA LLC

Disclosed herein is a single use continuous recovery, flow-through harvest vessel and corresponding method for harvesting culture medium and simultaneously either leaving the microcarrier beads behind in the vessel or flowing microcarrier beads and medium back into a bioreactor. 1. A bioreactor system , comprising:a nonporous vessel capable of holding a fluid, the nonporous vessel comprising:a planar top wall panel and a planar bottom wall panel joined directly to the top wall panel about an entire periphery of the top wall panel, the top wall panel and the bottom wall panel lying in registration with one another and forming a two-dimensional structure when in a non-expanded state, the top wall panel and the bottom wall panel being formed from a non-rigid flexible material, the top panel and the bottom panel defining an entire outer wall surface and an inner wall surface of the nonporous vessel, the inner wall surface defining an interior chamber for holding a fluid;a filter having a perimeter, a first surface and a second surface, and fixedly attached around its entire perimeter to the top wall panel the nonporous vessel, thereby forming an integrated interior culture medium bag within the nonporous vessel;a first fitment attached to the outer wall surface of the nonporous vessel at a portion of the outer wall surface that is adjacent to the integrated interior culture medium bag, the first fitment forming a first port allowing for fluid to flow out of the nonporous vessel through the first port after flowing through the filter and into the integrated interior culture medium bag; anda second fitment attached to the outer wall surface of the nonporous vessel at a portion of the outer wall surface that is not adjacent to the integrated interior culture medium bag, the second fitment forming a second port allowing for fluid to flow into the nonporous vessel and into the interior chamber;a third fitment attached to the outer wall surface of the nonporous vessel at a ...

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03-02-2022 дата публикации

LASER-ASSISTED REPOSITIONING OF A MICRO-OBJECT AND CULTURING OF AN ATTACHMENT-DEPENDENT CELL IN A MICROFLUIDIC ENVIRONMENT

Номер: US20220033758A1
Принадлежит:

Methods for laser-assisted repositioning of a micro-object and for culturing an attachment-dependent biological cell within a microfluidic device are described herein. Laser illumination is used to controllably create a bubble which repositions the micro-object. Further, methods of culturing an attachment-dependent biological cell are described, where the methods may include laser-assisted repositioning. 1. A method of repositioning a micro-object disposed in a chamber of a microfluidic device , comprising:directing laser illumination upon a starting position on an inner surface of the chamber to generate an illumination area, wherein:the microfluidic device comprises the chamber and a flow region containing a first liquid medium, wherein the chamber comprises an opening to the flow region, andthe starting position is disposed within a sub-region of the chamber distal to the opening to the flow region; andmoving the laser illumination relative to the microfluidic device, thereby changing the position of the illumination area such that, upon completion of the change, the laser illumination is at a final position, wherein the change in the position of the illumination area induces the repositioning of the micro-object at least towards the opening of the chamber;wherein moving the laser illumination relative to the microfluidic device controllably generates a bubble having a trajectory within the chamber, and wherein the generated bubble induces the repositioning of the micro-object.2. (canceled)3. The method of claim 1 , wherein the repositioning of the micro-object results in the micro-object being exported from the chamber into the flow region.4. The method of claim 3 , wherein the method further comprises exporting the micro-object from the flow region and out of the microfluidic device.5. The method of claim 4 , wherein the micro-object is a biological cell claim 4 , wherein the biological cell is exported out of the microfluidic device and into a culturing vessel ...

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15-01-2015 дата публикации

DEVICE AND METHOD FOR BACTERIAL CULTURE AND ASSAY

Номер: US20150017629A1
Принадлежит:

The present invention provides a simple culture device that is designed for manufacture and use in areas of limited resources. The device is useful for cell culture in such environments with limited resources because cells grow in paper just as they do in a culture dish. Also provided is a binding assay that employs an activatable dormant bacteriophage carrying a reporter gene to qualitatively or quantitatively detect the presence of a substance of interest in a sample. 1. A portable culture device comprising:a) a first culture strip comprising at least one culture region within a first strip of fluid impermeable material having an adhesive on its inner surface;b) a second culture strip comprising at least one culture region within a second strip of fluid impermeable material having an adhesive on its inner surface, wherein each of the at least one culture regions of the second culture strip corresponds to one of the at least one culture regions of first culture strip to form a culture region pair,wherein each culture region comprises an inner layer of porous hydrophilic material and at least one culture region in each culture region pair comprises an outer layer of a gas permeable membrane, andwherein the device is moveable between an open condition and a closed condition in which the first and second culture strips are releasably adhered via the inner adhesive surfaces such that the culture regions in each culture pair are aligned and the outer gas permeable membrane or gas permeable membranes are exposed to the environment to permit gas flow to the interior of the device.2. The device of claim 1 , wherein at least one of the culture regions comprises an adsorbent pad layered on the side of the porous hydrophilic material opposite the interior of the device.3. The device of claim 2 , wherein a dialysis membrane is layered between the adsorbent pad and the porous hydrophilic material.4. The device of claim 1 , wherein the first culture strip is connected to the ...

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15-01-2015 дата публикации

Stacked Membrane Bioreactor

Номер: US20150017683A1
Принадлежит:

Scalable biomaterial-based bioreactors are described. In one embodiment, the bioreactor may comprise perforated plates stacked such that the assembled bioreactor has the necessary manifolds and chambers to transport gas and liquids to a biomaterial contained within the bioreactor, and to remove the reaction products. In another embodiment, single use bioreactors are described. Methods of operating the bioreactors are also described. 1. A bioreactor , comprising:a biomaterial reactor chamber;a gas reactor chamber in fluid communication with a first side of the biomaterial reactor chamber;a medium reactor chamber in fluid communication with a second side of the biomaterial reactor chamber;a gas inlet and a gas outlet in fluid communication with the gas reactor chamber;a medium inlet and a medium outlet in fluid communication with the medium reactor chamber; and a flame arrestor, the flame arrestor being located in one or more of: the gas reactor chamber, the gas inlet, the gas outlet, the medium reactor chamber, the medium inlet, and the medium outlet; and', 'a rigid foam comprised by the gas reactor chamber., 'one or more of23-. (canceled)43. The bioreactor of claim , the biomaterial reactor chamber comprising a living organism capable of using hydrogen gas as a source of energy.5. (canceled)6Ralstonia eutropha.. The bioreactor of claim 1 , the biomaterial reactor chamber comprising7. The bioreactor of claim 1 , wherein the biomaterial reactor chamber includes a membrane assembly claim 1 , the membrane assembly comprising one or more of:a gas permeable membrane comprising one or more of: silicone, polyvinylidene difluoride (PVDF), porous polytetrafluoroethylene (PTFE), acrylic copolymer, polycarbonate, polypropylene, and modified nylon;a liquid permeable membrane comprising one or more of: woven material, cloth, mesh, glass fibers, nitrocellulose, mixed cellulose ester (MCE), polycarbonate, modified polycarbonate, polyethersulfone (PES), nylon, ceramic, PTFE, ...

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19-01-2017 дата публикации

Apparatus and Methods for Cell Culture

Номер: US20170015967A1
Принадлежит:

A bioreactor () for the culture of cells (C) comprising a stack of carriers () for cell (C) adherence and liquid medium (M) distribution. The carriers () are stacked so as to define levels () between adjacent carriers () for the flow of the liquid medium (M). Adjacent levels () are fluidly interconnected via open spaces () so that the liquid medium (M) can flow from one level () to an adjacent level (). The open spaces () between a first and an adjacent second level (′) do not overlap with the one or more open spaces () between the second level (′) and an adjacent third level (″). One or more of the carriers may also include an area adapted to prevent cell adhesion or growth, thereby allowing for the viewing of cell growth on adjacent carriers from a vantage point external to the bioreactor. Related methods are also disclosed. 117. A bioreactor () for the culture of cells (C) , comprising first and second carriers () each having at least one inlet for admitting a fluid medium , the first carrier including a first portion adapted for cell (C) adherence , a second of the carriers further including a second portion adapted to prevent cell adherence , whereby the second portion of the second carrier allows for viewing the first portion of the first carrier when the first and second portions align.217. The bioreactor () according to claim 1 , wherein the first and second portions comprise surfaces on the carriers ().31. The bioreactor () according to claim 1 , wherein the second portion includes an optically transparent material between the second carrier and the first carrier.417. The bioreactor () according to claim 1 , further including a single housing for receiving the carriers ().517. The bioreactor () according to claim 1 , wherein the carriers () comprise stackable trays or cubes.61. The bioreactor () according to claim 1 , further including a third carrier having the first portion adapted for cell adherence and a third portion adapted for preventing cell ...

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17-01-2019 дата публикации

A Rigid Housing for Holding a Flexible Bag

Номер: US20190015799A1
Принадлежит:

A rigid housing comprising a bottom part and at least one wall part, said bottom part and said at least one wall part together defining an internal volume when the bottom part is provided below the at least one wall part in a processing position, said rigid housing being arranged for holding a flexible bag within the internal volume, wherein the bottom part is rotatable about an axis of rotation, wherein said axis of rotation is substantially parallel to a longitudinal axis of said rigid housing such that the bottom part can be provided in a loading position in which the bottom part has been rotated out from the position below the at least one wall part. 1. A rigid housing comprising a bottom part and at least one wall part , said bottom part and said at least one wall part together defining an internal volume when the bottom part is provided below the at least one wall part in a processing position , said rigid housing being arranged for holding a flexible bag within the internal volume , wherein the bottom part is rotatable about an axis of rotation , wherein said axis of rotation is substantially parallel to a longitudinal axis of said rigid housing such that the bottom part can be provided in a loading position in which the bottom part has been rotated out from the position below the at least one wall part.2. A rigid housing according to claim 1 , wherein the flexible bag is a single use bioreactor.3. A rigid housing according to claim 1 , further comprising a joint connected to the bottom part and to the at least one wall part or to a stand connected to the at least one wall part claim 1 , said joint providing the axis of rotation about which the bottom part is rotatable.4. A rigid housing according to claim 1 , wherein the at least one wall part comprises at least one opening that allows for the transfer of liquid claim 1 , access to measuring of parameters or properties of the fluid internal to the bioreactor and/or coupling a mixer element internal to the ...

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21-01-2016 дата публикации

BIOREACTOR AND SENSING SYSTEM THEREFOR

Номер: US20160017269A1
Принадлежит:

The present invention relates to a measurement probe system () comprising a reusable unit, a pluggable electrical connector () and a disposable, single-use sensing unit (), the reusable unit comprising a connector support (), the disposable, single-use sensing unit () comprising a sealing sleeve () and a sensing device (). The sensing device () is configured to be fixedly connected to the sealing sleeve (). The pluggable electrical connector () comprises a connecting portion () configured to be connected to the connector support () and a complementary connecting portion (), which is connected to the sensing device () and to the sealing sleeve (). The complementary connecting portion () is configured to interact with the connecting portion () to provide electronic communication between the sensing device ()and the reusable unit, whereby the sealing sleeve () of the single-use sensing unit () is configured to removably receive the connector support () with the connecting portion (). 117-. (canceled)18. A measurement probe system for a bioreactor including a reusable unit and a disposable single-use sensing unit , the reusable unit comprising a rigid connector support and a pluggable electrical connector mounted on the connector support for connection to a measurement signal processing unit , the single-use sensing unit comprising a sealing sleeve hermetically sealing an inside of the sealing sleeve from an outside of the sealing sleeve configured to be in contact with a culture medium in a container portion of said bioreactor , a complementary electrical connector positioned in said inside of said sealing sleeve configured for releasable connection to the pluggable electrical connector , and a sensing device fixedly mounted to an outside of the sealing sleeve configured for contact with said culture medium , the sealing sleeve being configured to removably receive the pluggable electrical connector therein , and wherein an insulating support of the complementary ...

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18-01-2018 дата публикации

SUBSTRATES FOR HIGH-DENSITY CELL GROWTH AND METABOLITE EXCHANGE

Номер: US20180016533A1
Принадлежит:

A polymer or other substrate optimized for growing cells is described, which takes the form of a micro-thin bag with gas permeable sides. Sides of the bag can be held at a fixed distance from one another with a multitude of tiny micropillars or other spacers extending between them, keeping the bag at a predetermined thickness and preventing the bag from collapsing and the sides from sticking together. In other embodiments, the sides may be held apart by gas pressure alone. A 0.01 μm to 1000 μm parylene or other biocompatible coating over the bag outsides controls the permeability of the bag material and provides a bio-safe area for cell growth. An alternate configuration uses open-cell foam with skins coated with a biocompatible coating. Tubes going into multiple bags can be connected to a manifold that delivers gaseous oxygen or removes carbon dioxide and other waste gases. Multiple bags can be stacked together tightly, with o-ring spacers in between, and housed within a vessel to form a high-surface area, ultra-compact cell growing system. For cells growing on the bags, liquid nutrients can be fed by way of the tube spacers, and oxygen and waste gases permeated through the bag sides and transported within the bags. 1. A cell-growing substrate apparatus comprising:a pair of gas permeable, polymer sheets;an array of connection points between faces of the polymer sheets, the connection points having a center-to-center spacing less-than-or-equal-to 2000 μm;a hermetically sealed edge that bonds a perimeter of the sheets together, enclosing the connection points in an interior cavity between the sheets to form an expansion resistant bag;an inlet to the bag fluidly connected with the interior cavity; anda biocompatible coating over an outside of at least one of the gas permeable sheets, the biocompatible coating having a thickness between 0.01 μm and 1000 μm.2. The apparatus of wherein the center-to-center spacing of the connection points is between 100 μm and 1000 μm.3. ...

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21-01-2021 дата публикации

REINFORCED BIOREACTOR COMPONENT STRUCTURE SYSTEM FOR CELL CULTIVATION

Номер: US20210016287A1
Принадлежит:

The present invention involves a reinforced tubing structure that is particularly useful for reinforcing tubing that is present inside disposable bioreactor bags, including for example thermowell tubing. 1. A bioprocess bag comprising:a bag wall defining an enclosed volume for holding biomaterials, the bag wall comprising at least one inlet port and at least one outlet port; anda tube structure comprising a first opened-end proximate the bag wall and a second distal end, the tube extending into the enclosed volume, and the tube structure comprising a reinforced portion proximate the first opened-end.2. The bioprocess bag of claim 1 , wherein the second distal end is a closed-end claim 1 , and the tube is adapted to accept a probe for measuring one or more properties of the biomaterials.3. The bioprocess bag of claim 2 , wherein the probe is a thermocouple.4. The bioprocess bag of claim 1 , wherein the tube structure comprises a first opened-end proximate the bag wall and a second distal end extending into the enclosed volume claim 1 , the reinforced portion proximate the first opened-end.5. The bioprocess bag of claim 4 , wherein the tube structure is adapted to accept a sparging wand.6. The bioprocess bag of claim 1 , wherein the reinforced portion comprises a ribbed structure.7. The bioprocess bag of claim 1 , wherein the ribbed structure comprises a pattern of sections that are raised relative to the tube outer surface claim 1 , and the raised sections contacting each other when the tube structure is bent.8. The bioprocess bag of claim 7 , wherein the contact of the raised sections prevents crimping of the tube structure.9. The bioprocess bag of claim 1 , wherein the tube structure comprises a predominantly cylindrical inner wall claim 1 , and a notch extending at least a portion of the length of the inner wall.10. The bioprocess bag of claim 9 , wherein the notch defines a channel that allows air to flow out when a probe is inserted into the tube structure.11. A ...

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17-01-2019 дата публикации

FULLY AUTOMATED CONTINUOUS CELL CULTURE SYSTEM

Номер: US20190017009A1
Автор: Yu Xuejun

The invention provides a full-automatic cell culture system, at least comprising: a control module, a control platform, a dark field microscope for on-line observation of cell culture, a cell incubator shaker and a cell culture bag. The control platform is connected to the dark field microscope for on-line observation of cell culture and the cell incubator shaker respectively, and the control module is connected to the control platform. The invention also provides a culture bag support frame for a continuous cell incubator shaker, the continuous cell incubator shaker, a non-contact sensor connector, the cell culture bag and the dark field microscope for on-line observation of cell culture which are related to the full-automatic cell culture system, thereby realizing continuous culture and observation of cells. 1. A full-automatic cell culture system , characterized by at least comprising: a control module , a control platform , a dark field microscope for on-line observation of cell culture , a cell incubator shaker , a cell culture bag and a power source module; whereinthe cell incubator shaker comprises a culture bag support frame, a swing frame and a shake device, the culture bag support frame is provided with an arc-shaped bottom plate and cell culture bag fixing parts, the swing frame comprises a swing base, the swing base is provided with support arms, the culture bag support frame and the swing frame are fixedly connected by the support arms, the shake device comprises a substrate, the substrate is provided with swing components, the swing base is connected to the swing components, and the arc-shaped bottom plate is provided with an object lens inserting gap;the cell culture bag comprises a closed bag body, and the middle of the bottom surface of the bag body is provided with a transparent observation area; and the bag body is disposed on the cell incubator shaker, and the transparent observation area is matched with the object lens inserting gap;the dark ...

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21-01-2021 дата публикации

Photobioreactor with annular chambers

Номер: US20210017480A1
Автор: Narasimhan Sundaram
Принадлежит: ExxonMobil Research and Engineering Co

A photobioreactor includes one or more annular chambers concentrically positioned about a central axis, and an algae slurry contained within the one or more annular chambers.

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21-01-2021 дата публикации

System for Environmental Microbial Testing

Номер: US20210017481A1
Автор: Perlman Daniel
Принадлежит:

A gelled biological growth medium in a culture container, as well as kits and methods of producing the growth medium, utilize gelling of a liquid growth medium containing low methoxyl pectin on a film of calcium salt deposited on a growth surface of the culture container. The salt acts as a trigger for gelation and is conveniently deposited on the walls by evaporation of methanol or another volatile solvent. 1. A kit for preparing a gelled growth medium , the kit comprising:a) a liquid growth medium comprising a low methoxyl pectin material having less than about 8% methoxyl content; andb) a container for culturing microorganisms, wherein the container comprises a calcium lactate precipitate on a growth surface of the container.2. The kit of claim 1 , further comprising:c) instructions for coating the calcium lactate precipitate with a liquid growth medium to form a gelled biological growth medium.3. The kit of claim 1 , wherein the liquid growth medium is selected from the group consisting of potato dextrose broth claim 1 , yeast extract dextrose broth claim 1 , and combinations thereof.4. The kit of claim 1 , wherein the liquid growth medium further comprises an antibacterial agent.5. The kit of claim 4 , wherein the liquid growth medium comprises from about 50 to about 100 micrograms per milliliter of chloramphenicol as the antibacterial agent.6. The kit of claim 1 , wherein the container is a Petri dish.7. The kit of claim 1 , wherein the container for culturing microorganisms comprises from about 50 to about 350 mg of calcium lactate per gram of low methoxyl pectin in the liquid growth medium.8. The kit of claim 1 , which is suitable for preparing a gelled growth medium for selective growth of yeast or mold.9. A kit for preparing a gelled growth medium claim 1 , the kit comprising:a) a liquid growth medium comprising a low methoxyl pectin material having less than about 8% methoxyl content;b) a solution comprising calcium lactate and methanol; andc) a container ...

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21-01-2021 дата публикации

Unit for Treatment of a Bioprocess Liquid

Номер: US20210017482A1
Принадлежит:

The invention discloses a first unit () for treatment of a bioprocess liquid comprising a first lateral face (), a second lateral face () and a front face () which meets the two said lateral faces. The front face comprises: a plurality of valves () adapted to receive and act upon one or more legs () of a disposable flow path (); optionally one or more pumps () adapted to receive and act upon one or more legs of the disposable flow path; optionally one or more sensors () adapted to receive and to measure one or more parameters in one or more legs of the disposable flow path; wherein the plurality of valves and optional pumps and sensors are vertically offset from each other to give one or more legs of a disposable flow path received by said valves and optional pumps and sensors a slope of at least 3.0 degrees from the horizontal plane (h). 1. A first unit for treatment of a bioprocess liquid comprising a first lateral face , a second lateral face and a front face which meets the two said lateral faces; said front face comprising:a plurality of valves adapted to receive and act upon one or more legs of a disposable flow path;optionally one or more pumps adapted to receive and act upon one or more legs of said disposable flow path;optionally one or more sensors adapted to receive and to measure one or more parameters in one or more legs of said disposable flow path; whereinsaid plurality of valves and optional pumps and sensors are vertically offset from each other to give one or more legs of a disposable flow path received by said valves and optional pumps and sensors a slope of at least 3.0 degrees from the horizontal plane.2. The first unit of claim 1 , wherein said valves and optional pumps and sensors are arranged to give one or more legs of said disposable flow path a slope of 3.0-10.0 claim 1 , such as 3.0-8.0 degrees from the horizontal plane.3. The first unit of claim 1 , wherein said valves and optional pumps and sensors are arranged to give all legs of said ...

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21-01-2021 дата публикации

CELL EXPANSION SYSTEM

Номер: US20210017485A1
Автор: Lei Yuguo, Viljoen Hendrik
Принадлежит:

A cell expansion system for culturing and expanding cells in hydrogel tubes is disclosed herein. The cell expansion system allows for expanding cells that can significantly reduce the production time and cost, while increase the production capacity. 1. A cell expansion system for expanding cells , the system comprising:a cap comprising: an extruder comprising at least a first inlet and at least a second inlet, the first inlet operable for introducing a cell solution into the extruder, the second inlet operable for introducing a hydrogel-forming solution into the extruder; anda tubular housing in fluid connection with the extruder of the cap, wherein the tubular housing comprises a cell compatible buffer.2. The system of claim 1 , wherein the tubular housing further comprises a mesh.3. The system of claim 1 , wherein the cell solution comprises cells selected from the group consisting of mammalian embryonic stem cells claim 1 , mammalian induced pluripotent stem cells claim 1 , mammalian naive pluripotent stem cells claim 1 , cells differentiated from mammalian embryonic stem cells claim 1 , mammalian induced pluripotent stem cells and mammalian naive pluripotent stem cells claim 1 , mammalian cells reprogrammed from other cell types claim 1 , mammalian primary cells claim 1 , human umbilical vein endothelial cells claim 1 , cancer cells claim 1 , T cells claim 1 , mammalian tissue stem cells claim 1 , mammalian cell lines claim 1 , insert cells claim 1 , plant cells claim 1 , yeast and bacterial cells.4. The system of claim 1 , wherein the hydrogel-forming solution is an alginate solution comprising alginate polymer material selected from the group consisting of alginate acid polymers claim 1 , sodium alginate polymers claim 1 , modified alginate polymers claim 1 , and combinations thereof.5. The system of claim 4 , wherein the alginate solution comprises from about 0.01% (w/v) to about 20% (w/v) alginate.6. The system of claim 1 , wherein the hydrogel-forming ...

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16-01-2020 дата публикации

CELL CULTURE VESSEL, SAMPLE OBSERVATION CELL, AND CELL CULTURE METHOD

Номер: US20200017814A1
Принадлежит:

The present invention provides a cell culture vessel/sample observation cell that is capable of culturing cells or cell tissue in three dimensions and allowing a three-dimensional structure of the cells or the cell tissue to be perceived easily. The cell culture vessel/sample observation cell according to the present invention for containing a culture gel in which the cells or the cell tissue is embedded, comprises a frame, at least one window bordered by the frame, and at least one projection projecting from the frame inwardly into the cell culture vessel/sample observation cell, wherein the at least one window is light-permeable and nutrient component-permeable and is placed in such a way as to allow for multifaceted observation of the cells or the cell tissue, and the projection has a feature point to be placed at a position where the cells or the cell tissue as well as the feature point can be observed from the window. 1. A cell culture vessel for containing a culture gel in which cells or cell tissue is embedded , the cell culture vessel comprising a frame , at least one window bordered by the frame , and at least one projection projecting from the frame inwardly into the cell culture vessel , whereinthe at least one window is light-permeable and nutrient component-permeable and is placed in such a way as to allow for multifaceted observation of the cells or the cell tissue, andthe projection has a feature point to be placed at a position where the cells or the cell tissue as well as the feature point can be observed from the window.2. The cell culture vessel according to claim 1 , whereinthe at least one window is made from a hydrogel or a porous solid, andthe porous solid includes at least one of a porous material-made sheet, a mesh, an etching sheet, a non-woven cloth, and a woven cloth.3. The cell culture vessel according to claim 1 , wherein the frame is shaped like a cube or a rectangular parallelepiped claim 1 , and has an opening for placing the window ...

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16-01-2020 дата публикации

Cell culture substrate, culture vessel, method for producing cell culture vessel, method for acquiring cells and method for culturing cells

Номер: US20200017815A1

A cell culture substrate includes: a first layer that includes a first gel in which gold nanoparticles dispersed; and a second layer that includes a second gel in which the gold nanoparticles are not present or are present in a lower concentration in comparison with the first layer.

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16-01-2020 дата публикации

Well-plate incubator

Номер: US20200017817A1
Принадлежит: Berkeley Lights Inc

Incubators are disclosed which include an enclosure with an internal chamber configured to support a cell culture plate and provide an environment suitable for maintaining and/or culturing biological cells. The enclosure can include one or more openings configured to allow access to the cell culture plate. The incubators can further include a structure having a plurality of openings configured to be aligned with a corresponding plurality of wells in the cell culture plate, and a sealing element configured to moveably seal the plurality of openings in the structure. The sealing element can comprise a plurality of openings corresponding to at least a subset of the plurality of openings of the structure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the structure. Methods for using the incubators are also provided.

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16-01-2020 дата публикации

Methods for Culturing Cells in an Alternating Ionic Magnetic Resonance (AIMR) Multiple-Chambered Culture Apparatus

Номер: US20200017848A1

Provided herein are systems and methods for culturing tissue-like assemblies of cells in the presence of a pulsating alternating ionic magnetic resonance field. The cells are introduced into a growth module in fluid connection with a nutrient module in which the gravity vector of the growth module is continually randomized and cultured in the presence of the alternating ionic magnetic resonance field. 1. An alternating ionic magnetic resonance (AIMR) culture system for culturing a stable tissue-like assembly of cells , comprising: a first gas-permeable membrane disposed on a proximal end thereof;', 'a gas port fitted onto the first-gas permeable membrane; and', 'a first sealable opening on a distal end thereof; said distal end adaptable for an electrical connection;, 'a nutrient module, comprising a second gas-permeable membrane with a plurality of inlet/outlet ports thereon disposed on a proximal end thereof;', 'a baffling system on a distal end thereof; and', 'a semi-permeable membrane on the distal end thereof;, 'a growth module disposed within the nutrient module and fluidly connected with the first gas-permeable membrane thereof, comprisinga randomizing adapter comprising a randomizing mechanism in an electrical connection with the nutrient module received within an open proximal end thereof;a removable alternating ionic magnetic resonance chamber comprising an AIMR generating device with a diameter sufficient to receive at least the growth module therein.2. The AIMR culture system of claim 1 , wherein the nutrient module claim 1 , the growth module or a combination thereof are disposable.3. The AIMR culture system of claim 1 , wherein the nutrient module claim 1 , the growth module and the randomizing adapter are sterilizable.4. The AIMR culture system of claim 1 , wherein the AIMR generating device is configured to generate the pulsating alternating ionic magnetic resonance (AIMR) field with a frequency of about 7.8 Hz to about 59.9 Hz at fundamental ...

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21-01-2021 дата публикации

HIGH DENSITY 3D HEPATOCYTE SPHEROID PLATFORM FOR DRUG ADME STUDIES

Номер: US20210018492A1
Принадлежит:

The present disclosure relates to methods for evaluating the interaction of a candidate compound on 3D hepatocyte spheroid in an invitro culture, including evaluating the metabolism of a candidate compound, for use in various biochemical and molecular biology studies. The methods are performed in labware that combine 3D spheroid culture with micro-patterned design that allows for multiple to several hundreds of spheroids to be treated under the same conditions and to produce sufficient materials (e.g., parent drug, drug metabolites, DNA, RNA, and proteins from cells) and higher detection signal intensity for ADME/Tox (absorption, distribution, metabolism, excretion and toxicity) studies. The methods allow for, among other uses, the investigation and generation of accurate invitro intrinsic clearance data and thus more accurate prediction of in vivo clearance, particularly with low clearance compounds. 1. An assay method for evaluating the interaction of one or more low clearance candidate compounds with hepatocytes , comprising: 'a top aperture; and a liquid impermeable bottom comprising a bottom surface, wherein at least a portion of the bottom comprises a low-adhesion or no-adhesion material in or on the bottom surface;', 'culturing hepatocytes in a cell culture article to form a spheroid, wherein the cell culture article comprises a chamber, the chamber comprising an array of microcavities, each microcavity structured to constrain the hepatocytes to grow in a 3D spheroid confirmation to form a hepatocyte spheroid; wherein each microcavity of the chamber comprisescontacting the 3D hepatocyte spheroid with one or more low clearance candidate compounds; andmeasuring the in vitro intrinsic clearance of the one or more candidate compounds.2. The assay method of claim 1 , wherein the culture is a long-term culture.3. (canceled)4. The assay method of claim 1 , wherein the liquid impermeable bottom comprising the bottom surface is gas-permeable.5. The assay method of ...

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26-01-2017 дата публикации

AIR GAP FOR SUPPORTING CELLS

Номер: US20170020650A1
Автор: Rozy Yoram, Stern Yaki
Принадлежит: Beta O2 Technologies Ltd.

Apparatus is provided, including a housing configured for insertion into a body of a subject; at least one oxygen reservoir disposed within the housing; and at least one layer of transplanted cells, disposed within the housing; and gaseous oxygen disposed within the at least one oxygen reservoir. Other embodiments are also described. 1. Apparatus comprising:a housing configured for insertion into a body of a subject;at least one oxygen reservoir disposed within the housing;at least one layer of transplanted cells disposed within the housing; andgaseous oxygen disposed within the at least one oxygen reservoir, the apparatus arranged to permit passage of the gaseous oxygen from the at least one oxygen reservoir to the transplanted cells.2. The apparatus of wherein the transplanted cells comprise functional cells.3. The apparatus of wherein the functional cells comprise pancreatic islet of Langerhans cells.4. The apparatus of wherein the housing comprises a semi-permeable membrane having associated therewith a molecular weight cutoff claim 1 , the membrane being disposed with respect to the transplanted cells so as to protect the transplanted cells from components of a body fluid of the subject having molecular weights higher than the cutoff.5. The apparatus of wherein the membrane comprises gas-permeable membrane.6. The apparatus of wherein the at least one layer of transplanted cells comprises a first and a second layer of transplanted cells claim 1 , and wherein the first and second layers of transplanted cells are in communication with the gaseous oxygen.7. The apparatus of wherein the apparatus is configured to permit passage of the gaseous oxygen between the at least one oxygen reservoir and a vicinity in the body of the subject that is external to the housing.8. The apparatus of further comprising an oxygen generator.9. The apparatus of wherein each of the at least one layer of transplanted cells comprises a metal grid which houses a substrate to which the ...

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17-04-2014 дата публикации

COMPOSTER

Номер: US20140106443A1
Автор: Adam Robert James
Принадлежит: Global Environment Management (FZC)

A composter comprising a container for receiving material to be composted, an aerator within the container having at least one opening for allowing air to pass from the aerator into the container, a base structure defining a floor on which the composting material sits above a leachate tank located under the floor, an air flow path through the base structure for providing air from outside the composter to the aerator and to openings located in the floor above the leachate tank to facilitate aerobic decomposition of the composting material. 1. A composter comprising a container for receiving material to be composted , an aerator within the container having at least one opening for allowing air to pass from the aerator into the container , a base structure defining a floor on which the composting material sits above a leachate tank located under the floor , an air flow path through the base structure for providing air from outside the composter to the aerator and to openings located in the floor above the leachate tank to facilitate aerobic decomposition of the composting material.2. The composter according to wherein the base structure includes a plurality of spaced pillars that support the underside of the floor claim 1 , the pillars facilitating air flow through the floor.3. The composter according to wherein the pillars extend upwardly from the base of the leachate tank.4. The composter according to wherein each pillar has a cut out in its wall facilitating air flow into the leachate tank.5. The composter according to wherein the base structure includes a perforated floor panel which in use supports the composting material.6. The composter according to wherein an air inlet allows air to enter the composter and flow through one or more air channels beneath the floor and enter the container through one or more holes in the floor.7. The composter according to wherein conduits direct air from beneath the floor to the holes in the floor. Composting is an environmentally ...

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28-01-2016 дата публикации

DEVICES TO EXPOSE CELLS TO FLUID SHEAR FORCES AND ASSOCIATED SYSTEMS AND METHODS

Номер: US20160024454A1
Принадлежит:

The present technology relates generally to devices to expose cells to fluid shear forces and associated systems and methods. In particular, several embodiments are directed toward devices to expose cells to fluid shear forces in order to measure changes in internal cell forces. In some embodiments, a fluidic device includes a flow unit configured to induce fluid flow through the device. The device further includes a fluid channel configured to accept a biological sample dispersed on an array of flexible structures. The flow unit can be configured to induce disturbed and/or laminar flow in the fluid channel. The device can further include optical or magnetic detection means configured to measure a degree of deflection of one or more flexible structures in the array. 1. A fluidics device , comprising:a flow unit configured to induce fluid flow through the device;at least one a fluid channel configured to accept a biological sample dispersed on an array of flexible structures, wherein the fluid channel is configured to induce at least one of disturbed or laminar flow in the fluid channel; anda detection means configured to measure a degree of deflection of one or more flexible structures in the array.2. The device of claim 1 , further comprising at least one of an air chamber or a step positioned in the fluid channel and configured to control a characteristic of fluid flow through the array.3. The device of wherein the biological sample comprises protein solutions claim 1 , tissues claim 1 , cells claim 1 , or fragments of tissues or cells.4. The device of wherein the biological sample comprises at least one of whole blood claim 1 , platelets claim 1 , endothelial cells claim 1 , tumor cells claim 1 , fibroblasts claim 1 , smooth muscle cells claim 1 , cardiomyocytes claim 1 , white blood cells claim 1 , red blood cells claim 1 , or fragments thereof.5. The device of wherein the flexible structures comprise microposts made of silicon claim 1 , polymers claim 1 , metal ...

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