COMPOSITION IN FORM OF ULTRASONIC-DISPERSED NANOEMULSION HAVING ANTIOXIDANT ACTIVITY
The present invention refers to nanoemulsions type composition having anti-oxidant activity relates to more particularly rosemary extracts mixed with a number obtained by emulsifying and tocopherol after ultrasonic treatment, antioxidant activity and 100 provided 400 nm has a particle size of nano emulsion compositions are disclosed. Grain and seed oil as natural antioxidant number representative tocopherol (Seed oil) which radiates green or preventing much distribution, higher order δ > γ > β>α antioxidant force. Functional groups constituting the small take-unsaturated fatty in cells made readily detectable by free radical oxidation which, tocopherol oxidation process such interruption of the free radicals and oxidative protection against damage for comprehensively take number to other. However tocopherol in oxidation stability effect of vegetable oil direction of a low-priced price herbal compensations the spirit. Rosemary (Rosmarinus officinalis l.) has strong flavor is perfume from hub mechanism, we shall be used for medicine, deodorizing number, etc. also reports the number vice-direction as food flavoring agents. In addition, ceramic etc. etc. strong antioxidant action. The major constituent of dietary it buys (carnosic acid) rosemary anti-oxidation activity, such as the ends of the first carousel least one d (carnosol), most of these polyphenol ingredient a number to produce a stable active hydrogen atoms made active oxygen free in D curlunder public affairsexpense D curl number exhibits antioxidation effects by industry. Recently rosemary extracts used polished etc. antioxidant preventing the acidification maintained for foods. A compensation registration patent number 10 - 1993 - 0027690 call 'antioxidant number number composition and maintaining food of thick plate method' is thus strong antioxidation effects known as active ingredient having green tea extract and d - delta tocopherol antioxidant composition obtained by mixing a number of black pine tocopherol antioxidant synergistic effect has been the first input stage are described. However said patent antioxidation effects but green tea extract used in the formula, to be used as a food ingredient car reel [khin[khin] this oxygen and by means black discoloration appears to be specific green tea has the strong in aroma motor are disclosed. In addition a compensation publicized patent 10 - 2009 - 0003602 call 'number of antioxidant manufacturing method' car reel [khin[khin] withpak lung number using number powder was mixed natural antioxidant in yeast tocopherol and high pressure liquid coolant. However car reel [khin[khin] layer is decreased the amount of increased surface brightness which is red and blue aspect said that exhibit corrosion disclosed. In addition a compensation publicized patent number 10 - 2004 - 0070729 call 'inhibiting browning number and manufacturing method' when used as same as number 5% green tea extract in inhibiting browning but also through the colored a predetermined color of extract having intensified dipping solution surface within the robbery. Said green tea extract of the existing method such as that described by mixing techniques have made it difficult tocopherol antioxidant synergy effect causing discoloration during storage along the environment but latent and after fixed period of 60/40 titania fixed force phenomenon and not dissolved in tocopherol emulsion address as substrate. Method for nanoemulsions can be divided into high energy sampled that energy law form number is high pressure liquid coolant. High energy method is ultrasonic treatment, such as high pressure homogeneous method (High-a pressure Homogenizer) which, that energy law magnetic coupling is emulsified, inversion emulsion method, etc. spontaneous emulsion. The method machine having a device for emulsifying material energy strong destructive power method using various properties oil emulsion number although use of industrially and easy, requiring the lower portion of the powder disclosed. By using the relatively simple method without expensive that energy law number but not the oxide, it mainly synthetic emulsion number must be available a rear industrial production may have the disadvantages described. In the present invention one of ultrasonic energy method using a high pressure liquid coolant nanoemulsions his number. The use of high pressure homogeneous light (High-a pressure Homogenizer) when high mark Japanese visit, cause can be occur, damage parts fine metal powder jet can be included. In addition number due to high temperature and high pressure properties of the color change in the article can be turned into high change in 2000. While, ultrasonic cavitation bubbles into the ultrasonic decodes the aqueous solution of very high temperature and pressure generated when bubbles are broken and shock wave of high temperature and high pressure acts in the high energy source is installed under the oxide powder is heterogeneous liquid intermingled or nano level ultrasonic and apparatus for finely dispersed can be made to other. The intensity of the ultrasonic frequency of output of approximately 100 a-400W 20 a-28kHz degree is measured for plural times. The small particle size of different but along the nucleotide of such emulsion 20 provided 500 nm has a transparent or translucent visible shown substrate. Since particle size is very small and can not affected in coagulation actual condition, emulsion aggregation of not maintaining long-term stable state can be heated. The current ultrasonic cosmetic emulsion techniques in particular are arranged one of stability, uniformity, etc. high interface area has a cone-shaped skin absorption and a penetration force by a second service with the lower disclosed. In addition to ointment, photographic film of emulsion as well as, mayonnaise, chocolate, etc. even bath like number widely applicable. The emulsion stability and to elevate the synergistic effect in the present invention, green tea extract of rosemary extracts instead agent, such as a high pressure liquid coolant using prior art antioxidant number when said number, in food absorption and a penetration force are expected to display anti-back height. In order to solve said door number point such as, in the present invention rosemary extracts maximize absorption and a penetration force of the optimum and tocopherol antioxidant activity defoamer maximizes nanoemulsions form of water-soluble antioxidant number pin is for development. In addition, the present invention grain from Serial, nuts processed food as well as a wide variety of food, cosmetic, feed, hereinafter used to number of the first and second for perfume in the pin is a water-soluble antioxidant. In order to achieve said purposes, the present invention refers to rosemary extracts, tocopherol and emulsion including a number, nano-emulsion compositions having anti-oxidant activity number under public affairs substrate. The compositions of the present invention according to nano emulsion foods and cosmetics, feed water soluble for penetration force hereinafter accumulates the high number sheet in the field and, in particular, there is provided a workpiece or unsaturated fat content grain in quality due to a workpiece nuts automatic oxidation number equal to or less than extend preparing billion. Example 3 and comparison example 4 to 24 35 °C in comparison with Figure 1 in the embodiment 12 a graph indicating a change in the daytime storage Serial of a peroxide the creation which will bite are disclosed. The present invention refers to rosemary extracts, tocopherol and emulsion including a number, nano-emulsion compositions having anti-oxidant activity number under public affairs substrate. The present invention according to composition rosemary extracts, tocopherol and emulsifier mixture including a number can be obtained by an ultrasonic treatment. According to an implementation of the present invention, composition of the present invention mixed tocopherol rosemary extract and homogenizing step; (2) glycerin and emulsified in a number additional mixing said mixture; and (3) said mixture prepared by emulsion forming a number by an ultrasonic treatment bath can be. The compositions of the present invention selected from the rosemary extract is dried rosemary leaves, a suitable solvent, for example ethanol or possible to be a extract of white. For use in the present invention the extract or rosemary extract appropriate edible oil or fat mixed with cream or be a stand-alone number after solvent extract. 4 to 15% by weight to said rosemary extract including roh carit buys this preferably. The tocopherol compositions of the present invention using any one known contrast but, to obtain excellent antioxidation activity d α, β, γ, including 50 to 95% by weight preferably to δ - tocopherol. Compositions of the present invention emulsions number is the polyglycerol fatty acid ester, sugar fatty acid ester, brush grief region it buys S reel [lu[lu], lecithin and combinations thereof can be selected from the group consisting of. According another embodiment of the present invention, composition of the present invention can be further comprises glycerin. According to an implementation of the present invention, 9 to 25% by weight of rosemary extracts and tocopherol composition of the present invention, emulsion number 0. 5% by weight to 5% by weight glycerin and comprising 65 to 85 can be. The embodiments of the present invention, 9% by weight to 25% by weight of the present invention composition rosemary extracts mixing and homogenizing step 9 to 25 and tocopherol; (2) said mixture emulsified in a number 0. 5 65 to 85% by weight to 5% by weight glycerin and further mixing; and (3) said mixture prepared by emulsion forming a number by an ultrasonic treatment bath can be disclosed. A manufacturing method of the present invention also creating ranges. Number of the present invention composition includes a water soluble antioxidant as food, cosmetic, feed or feed can be used to perfume. In one example, based on the total of food composition of the present invention 0. 01 to 2 weight % peroxide generation delay any additive effect can be included in the amount of food. Preferred embodiment hereinafter of the present invention to aid in understanding, and positive examples in experiment number-gate could be compared. Only, to aid in the understanding of the present invention which includes a number without changing the security related in subject matter of invention, hereinafter of one embodiment example number is not. In the embodiment 1: rosemary extracts number bath 2 rosemary extract is rosemary leaves during drying diameter 0. 5 to 5 mm was manufactured. Grinding a 1:9 ethanol added at a temperature of 80 - 99% rosemary processed a predetermined number 1 to 40 °C 24 to 30 hours then ethanol extract concentrated powder phase difference said 1 bath tea extract are obtained. Said rosemary concentrated powder added to a predetermined 1:9 and edible oil or fat in 100 °C homodyne mixing (2,000 rpm, 30 minutes) when the number of holes in the same rosemary extract high pressure liquid coolant to each other. 4 to 15% by weight and comprising said active ingredient dietary it buys rosemary extract. Rosemary extracts sold for food and said condensed water is formed even when used rosemary extracts independent disclosed. In the embodiment 2: tocopherol preparation Tocopherol (mixed) d - tocopherol (number irradiation: Worldbestve) active ingredient into condensed water is formed sales number products α, β, γ, comprising 50 to 95 weight % and the total tocopherol content δ configured as disclosed. Experiment example 1: mixture design program using rosemary extracts derived mixture ratio and tocopherol In the embodiment 2 in the embodiment 1 and optimized in order to derive a ratio mixture design program (Design expert 8, star-a Ease, inc.) by water. Mixture is added to water soluble materials for elder brother anger emulsions number number combination utility in the polyglycerol fatty acid ester, sugar fatty acid ester, brush grief region it buys S reel [lu[lu], lecithin and combinations thereof selected from the group consisting of at least one emulsion as number 1, the blended amount of pre-test oil number 1. 5% by weight according to the fixed variable, 0 to 30% by weight in the embodiment 2 in the embodiment 1 and combination of each range are set to. This emulsifying material (oil in water) for switching on the utility water-on number of the emulsifiers are water, which is important to ensure that the ratio of the emulsion, the emulsion is 30% by weight when a blended amount of unstable emulsion which affects the physical properties as signal peptides. 65 to 85 weight % remaining glycerin are set to. Said combination mixture design program such as input range have, been such as to table 2 8 mixing compounding is derived. In the embodiment 3 in the embodiment 10 of water-soluble antioxidant blended in such a proportion of 2 to table based on the number to number after high pressure liquid coolant to a homo-mixer (3,000 provided 5,000 rpm, 15 - 30 minutes), evaluating oxidation stability AOM test IC curl purge activity antioxidant force on lighting equipment50 (DPPH) analysis was embodiment. Preparing of a fat measuring method is one of several AOM test 'The active oxygen method' soft-air and heat retaining plate of the number of the injection time maintained on the oxidized, i.e. induction period duration measuring method induced effect due to oxidation stability excellent disclosed. Table 1 analysis of AOM test for conditions such as disclosed. DPPH is oxidized forms free d curl have on obtaining reduction has an electron donor chain antioxidant number electronic meet with each other. DPPH is oxidized forms when the artificial pearl and a violet, the displayed roh and color preparing an additive reduction approaches other. DPPH extinction price 517 nm (OD) reacting in solution sample (antioxidant number) after (%) measured lighting equipment curl purge actinic value by using predetermined material. IC50 (Inhibitory Concentration of 50%) concentration 50% as the concentration of free d curl erase required value can comprise antioxidant material having excellent disclosed. <Violated> (%)=(matching as much as nine light absorptions - sample absorbance)* 100/(matching as much as nine light absorptions) curl purge actinic value lighting equipment Said table 2 the results of experiments, rosemary extracts contain only (in the embodiment 1) in the embodiment 8 is a low and high oxidation stability while lighting equipment curl purge activity, tocopherol (in the embodiment 2) in the embodiment 9 contain only a high oxidation stability while his curl purge activity lighting equipment is low. In addition rosemary extracts when mixed and tocopherol, oxidation stability and lighting equipment curl purge activity those is corrected has been confirmed. (In the embodiment 7) based on the ratio of the oxidation stability of a fat curl purge activity IC according to same and tocopherol rosemary extracts and lighting equipment50 Value mixture design program model analyzed was the fatigue. Maintaining the predetermined optimum ratio for analyzing conditions in oxidation stability is most induction period longer lighting equipment curl purge activity IC50 Concentration of a silicon wafer is maximum setting to a minimum whilst the antioxidants. Said analysis results shown in table 3 represented. In the embodiment 11: nanoemulsions number using ultrasonic bath Said table 3 in the embodiment 2 in the embodiment 1 and accurately meter the optimum mixture ratio based on at least one of mixing and homogenizing adding emulsion number 1, a high pressure liquid coolant compensation department agitating his number. Said ultrasonic dispersivecompensation department (Sonic yarn) 55 provided 60 °C using 15 minutes while adding rape oil slowly to maintain 30W are agitated during the ultrasonic wave, cooling the high pressure liquid coolant nanoemulsions his number. Comparison example 1: high pressure homogeneous light using emulsion number bath (High-a pressur Homogenizer) Table 3 in the embodiment 2 in the embodiment 1 and accurately meter the optimum mixing ratio based on said homogenizing a mixture adding paper number 1 internal phase emulsions, agitating a number 1 difference compensation department his high pressure liquid coolant. Said compensation department rape oil slowly after stirring 2 to maintain a pressure difference by adding 55 provided 60 °C homogeneous light (800bar) number emulsion was prepared by using high pressure liquid coolant. Comparison example 2: homo mixer using emulsion number bath Said table 3 in the embodiment 2 in the embodiment 1 and accurately meter the optimum mixture ratio based on at least one of mixing and homogenizing adding emulsion number 1, a high pressure liquid coolant compensation department agitating his number. Said compensation department a homo-mixer (3,000 rpm, 10 - 20 minutes) to maintain 55 provided 60 °C while adding rape oil slowly stirring, emulsion high pressure liquid coolant cooling his number. Experiment example 2: particle size and number on measuring it burns before emulsions Manufacturing method according to example 1 and comparison example 2 compared to make sure that the particle size of said in the embodiment 11 number 3 have repeated high pressure liquid coolant, particles using emulsion particle size and number seal (SZ-a 100, HORIBA yarn _JAPAN) it burns beforeabove 5 times each average value shown by measures. In the embodiment 11 1 formed in said table such as 400 nm hereinafter know nanoemulsions of example 4 were compared. In addition number aboveit burns before determination result, stable emulsion that is formed purges. In particular in the embodiment 11 unless a significant difference compared to the example 1 and the average particle size were cylindrical. As a result of the ultrasonic number using said high pressure liquid coolant homogeneous light crossroad number finer particles than high pressure emulsion emulsion high pressure liquid coolant has been confirmed that the emulsion formed. Experiment example 3: mixing ratio of optimal antioxidation verification Example experiment 1 derived based on said ratio to verify that the optimum predicted efficacy compared to example 1 in the embodiment 11 number number emulsions using high pressure liquid coolant homo mixer external grudge said testing method example 1 have the same measured, the result is shown in the table 5. In the embodiment 11 such as example 1 efficacy compared said table 5 anti-verification, the comparison example 3 table 1 differs about the expected efficacy, not significant. While, in the embodiment 11 in a usual expected efficacy but the oxidation stability, in comparison example 1 low IC about 15% active erase a radical50 Value higher active and viscoelasticity. Experiment example 2 as in the embodiment 11 is found in fine nanoemulsions state formed by selectively etching from oxidation or better efficacy determined substrate. Experiment example 4: grain Serial number article applied test Example 3 compared to example 1 in the embodiment 11 1 derived based on said experiments when subjected to product Serial number of grains, preparing a filler therefrom. 6 grain Serial number such as bright Joe is based and incense 4700. First, washed clean after about 5 time immersed in the manufactured based on putting into a little 80 provided 90 °C root was about 1 temporal extent. After root 55 °C dry in an oven 24 1 time difference very dry. Using said dried grain roller 0. 3 mm size molding by extruding, about 175 °C 5 minutes age. It developes [lep[lep] was installed at high pressure liquid coolant to the long grain by combining a number after spraying, while his hot blast heater coating. Grain drying said coated with sugar syrup was a final grain Serial number difference 2 high pressure liquid coolant. In the embodiment 12 prepared by the number, comparison example 3, example 4 - 35 °C compared to keeping a constant temperature polyolefin, 0, 6, 12, 18, 24 and the main disclosed take out an ether solvent extracting program is maintained by measuring the change of peroxide generating daytime 24 have, mistletoe to result table 7. Calculating method><peroxide prices (POV) 1. 1. (Meq/kg)={(a-a b) x f/kit for sampling amount (g)} peroxide prices x 10 A: 0. 01N sodium thiosulfate consumption (ml) B: public experiment in 0. 01N sodium thiosulfate average consumption (ml) F: 0. HBV DNA replication of 01N sodium thiosulfate Should be<: idle>food Said table 7 daytime of a peroxide water producing change measured such as 24, in the embodiment 12, comparison example 3, example 4 6 until both comparison difference increased sight without peroxide opening parking prices change. In the embodiment 12 example 3 in comparison with expanded storage 24 near the walls 12 but after parking difference compared to example 4 about 36% compared to the parking, 27% by low levels has been confirmed. In particular in the embodiment 12 is ultrasonic maximize grain-eating insects in particulate form using nano pore penetration force is provided to increase the surface area and application, comparison example 3 antioxidant than the effect that delay generation prices peroxide has excellent efficacy are judged. The present invention relates to a composition in a nanoemulsion form with a particle size of 100-400 nm and antioxidant activity, containing a rosemary extract, tocopherol, and an emulsifier. The composition shows a synergistic effect in antioxidation from the rosemary extract and tocopherol, can easily penetrate into an application object by having a nanoemulsion form, and has a superior antioxidant effect by the maximized application surface area. The composition of the present invention is capable of effectively controlling and delaying the acidification of food rich in unsaturated fatty acid such as grain cereals, nuts, etc., cosmetics, animal feed, spices, or the like. COPYRIGHT KIPO 2017 Nanoemulsions having anti-oxidant activity in the form of composition, 9 to 25% by weight to 25% by weight of rosemary extracts and tocopherol 9 by mixing and homogenizing step; said mixture emulsified in a number 0. 5 65 to 85% by weight to 5% by weight glycerin and further mixing; and said mixture to form an ultrasonic treatment nanoemulsions; and from the synthesized on a base emulsion compositions. According to Claim 1, 4 to 15% by weight and said roh carit buys this rosemary extract, said tocopherol d α, β, γ, δ - 50 to 95% by weight isopropanol compositions including a characterized. According to Claim 1, said emulsion number is the polyglycerol fatty acid ester, sugar fatty acid ester, brush grief region it buys S reel [lu[lu], lecithin and combinations thereof selected from the group consisting of composition characterized. Anti to number 1 according to Claim 3, said composition food, cosmetic, feed or feed used to perfume characterized composition. Device model 892 Professional Rancimat Sample weight 3. 0g Temperature 100 °C Pneumatic amount 20 L/h Result calculation Induction period (hr) Classification In the embodiment 1 (% by weight) In the embodiment 2 (% by weight) Glycerin (% by weight) Emulsion number (% by weight) Oxidation stability (induction period, hr) IC curl purge activity lighting equipment50 (Μg/ml) In the embodiment 3 18. 18 6. 06 74. 25 1. 50 15. 74 617. 0 In the embodiment 4 18. 5 0. 00 80. 00 1. 50 15. 64 2,285. 0 In the embodiment 5 6. 06 18. 18 74. 25 1. 50 14. 78 258. 5 In the embodiment 6 0. 00 24. 25 74. 25 1. 50 14. 02 208. 5 In the embodiment 7 15. 00 15. 00 68. 50 1. 50 15. 75 300. 5 In the embodiment 8 30. 00 0. 00 68. 50 1. 50 16. 01 1,400. 0 In the embodiment 9 0. 00 30. 00 68. 50 1. 50 14. 53 163. 5 In the embodiment 10 0. 00 18. 50 80. 00 1. 50 15. 5 249. 0 In the embodiment 1 (% by weight) In the embodiment 2 (% by weight) Glycerin (% by weight) Emulsion number (% by weight) Expected efficacy Oxidation stability curl purge activity lighting equipment 17. 2 12. 8 68. 5 1. 5 15. 86hr 290. 27 μg/ml Classification Particle size (nm) On it burns before number (mV) In the embodiment 11 216. 3 -35. 21 Comparison example 1 395. 5 -33. 15 Comparison example 2 783. 6 -20. 37 Classification Oxidation stability (hr) A radical erase royal tomb activity IC50 (Μg/ml) In the embodiment 11 16. 23 260 Comparison example 1 16. 10 300 Classification Raw material Ratio (% by weight) In the embodiment 12 Comparison example 3 Comparison example 4 Fresh water fish Brown rice 40 40 40 Corn 30 30 30 Barley 14. 7 14. 7 14. 7 It developes Sugar 10 10 10 Salt 0. 3 0. 3 0. 3 Be positive number 4. 9 4. 9 5. 0 In the embodiment 11 0. 1 - - Comparison example 1 - 0. 1 - total 100 100 100 POV (meq/kg) The storage 0 main 6 main 12 main 18 main 24 main In the embodiment 12 20. 83 25. 65 30. 14 37. 25 43. 59 Comparison example 3 20. 94 27. 65 35. 98 45. 36 57. 74 Comparison example 4 22. 10 30. 63 39. 28 52. 72 68. 37
