Swine listeriosis, swine necrobacillosis and swine francisella tularensis tri-combination detection test paper strip
The invention relates to a detection appliance for three-kind swine bacterial diseases, and in particular relates to a swine listeriosis, swine necrobacillosis and swine francisella tularensis rapid diagnostic test paper strip. The test paper strip contains a support layer and a reaction reagent carrier adsorption layer; the support layer is a non-water-absorbing thin sheet strip, and the reaction reagent carrier adsorption layer is pasted on the support layer; a fiber layer, a three-kind bacterial disease antigen gold-labeled monoclonal antibody or polyclonal antibody fiber layer, and a cellulose membrane layer are successively arranged from a sample test end, and a handle end adopts a water-absorbing material layer; detection imprints of '|||', '///' or '\\' are respectively sprayed on the fiber layer with a three-kind bacterial disease antigen matched monoclonal antibody or polyclonal antibody or monoclonal antibody solution; a contrast imprint of '|', '/' or '\' is sprayed on the cellulose membrane layer with a sheep (rabbit) anti-mouse or anti-pig IgG polyclonal antibody or an SPA solution. The detection test paper strip is specific, sensitive, intuitive, accurate, simple and rapid, and can be popularized and applied in livestock and poultry breeding, meat processing and quarantine and other related departments. 1. A detection Pig li bacillosis , Pig Bad spiritless colibacillosis pig earth decorah colibacillosis and the three joint inspection test ddia, the test strip containing support layer and the adsorption layer, the support layer is a non-bibulous foil layer, the adsorption layer is attached on a support layer, the adsorption layer of the sample from the testing end of the fiber layer, a gold-labeled antibody fiber layer, cellulose film layer and the handle end of the water-absorbing material layer, in the cellulose film to prepare a detection engram and the contrasting engram, gold-labeled antibody is characterized in that a layer of particulate labels nanometer level goldli bacillosis anti-Pig, Pig Bad spiritless spiral and pig earth decorah colibacillosis in three specific antigen of a pathogen monoclonal antibody, detection engram li bacillosis with anti-Pig, Pig Bad spiritless colibacillosis pig earth decorah colibacillosis and the pairing of pathogen-specific antigen monoclonal antibody or polyclonal antibody printed, contrast engram IgG with sheep or rabbit anti-mouse polyclonal antibody or the Staphylococcus aureus protein A printed (SPA). 2. Test ddia according to Claim 1, which is characterized in that a gold-labeled antibody fiber layer of particulate labels nanometer level goldli bacillosis anti-Pig, Pig Bad spiritless colibacillosis pig earth decorah colibacillosis and pathogen specific antigen of the mixed solution of three kind of monoclonal antibody, detection engram li bacillosis with anti-Pig, Pig Bad spiritless colibacillosis pig earth decorah colibacillosis and the pairing of pathogen-specific antigen monoclonal antibody or polyclonal antibody printed, contrast engram IgG with sheep or rabbit anti-mouse polyclonal antibody or the Staphylococcus aureus A protein (SPA) printed, or gold-labeled antibody fiber layer has the particle nanometer level goldli bacillosis a labelled anti-Pig, Pig necrosis pathogen pig earth decorah colibacillosis and colibacillosis in the polyclonal antibody, detection engram respectively li bacillosis with anti-Pig, Pig Bad spiritless colibacillosis pig earth decorah colibacillosis and pathogen specific antigen monoclonal antibody preparation, contrast engram Staphylococcus aureus A protein (SPA) or anti-swine IgG multiresisitance preparation. 3. Test ddia as in Claim 1 or Claim 2, characterized in that detection engram li bacillosis with anti-Pig, Pig Bad spiritless colibacillosis pig earth decorah colibacillosis and pathogen specific antigen of matching imab preparing ready-to-use the above-mentioned three kinds of pathogen-specific antigen of preparing the matching imab solution respectively; detecting engram li bacillosis with anti-Pig, Pig necrosis pathogen pig earth decorah colibacillosis and colibacillosis in the polyclonal antibody preparation that is, the above-mentioned three kinds of pathogens for polyclonal antibody preparation, respectively. 4. Test ddia according to Claim 1, is characterized in that the support layer is of hard non-absorbent article is made of plastic or hard paper; test end is made of glass wool for sample adsorbing layer; layer for gold-labeled antibody is made of glass wool and a gold-labeled antibody, gold labeling antibody can be a monoclonal antibody or polyclonal antibody. 5. Test ddia according to Claim 1, characterized in that cellulose nitrate film for cellulose film, or pure cellulose film, or carboxylated cellulose membrane, of cellulose membrane or polyvinylidene fluoride is made of PVDF. 6. Test ddia according to Claim 1, which is characterized in that a layer of water absorbent material is made of absorbent paper. 7. Test ddia according to Claim 1, which is characterized in that the detection engram and the contrasting engram to linear, or oblique-line, the cellulose film containing three detection engram and a contrast engram, detection engram and the contrasting engram the arrangement of the form "| | | |", "/ / / /" , in "\ \ \ \ " any one of the. 8. Test ddia according to Claim 1, which is characterized in that the test strip adsorption layer containing a layer of the protective layer, the protective layer attached to the adsorption layer, the adsorption layer of the sample at the test end, a gold-labeled antibody fiber layer and a layer of water absorbent material is covered with a protective film, the absorption of the sample at the test end with a gold-labeled antibody at the layer of the protective film layer corresponding to the mark lines are printed on the sample, the marker-line deflection test end of the one side of the sample absorption fiber layer about 0.5 cm is.